CN105993283A - Method for accelerating germination of seedlings of K. latifolia'Bridesmaid' - Google Patents
Method for accelerating germination of seedlings of K. latifolia'Bridesmaid' Download PDFInfo
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- CN105993283A CN105993283A CN201610456098.0A CN201610456098A CN105993283A CN 105993283 A CN105993283 A CN 105993283A CN 201610456098 A CN201610456098 A CN 201610456098A CN 105993283 A CN105993283 A CN 105993283A
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- seed
- bridesmaid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a method for accelerating germination of seedlings of K. latifolia'Bridesmaid'. The method comprises steps as follows: seeds are disinfected for 15-25s with 70%-80% ethanol firstly; the seeds are taken out and washed with distilled water for 3-5 times; then the seeds are soaked in warm water at the temperature of 35-45 DEG C, and the seeds after soaked for 15-40 min are taken out and placed in a germinating box with a layer of germinating paper; the germinating box accommodating the seeds is arranged in a constant-temperature illumination incubator with the illumination intensity being 3,000 xl, the illumination time being 12 h and the temperature being 25 DEG C for cultivation until the seeds are completely germinated. Besides, the seed soaking effect of a gibberellin solution is better than that of the warm water. According to the technical scheme, the germination rate of the seeds of K. latifolia 'Snowdrift' an be remarkably increased to about 80% from original 40% or lower.
Description
Technical field
The present invention relates to the method that a kind of bridesmaid's of quickening mountain laurel sowing seedling is emerged, belong to Woody flower cultivation technique
Field.
Background technology
Mountain laurel (Kalmia latifolia L.) be Ericaceae (Ericaceae) kalmia plant, main
Want branch in U.S. Appalachian Mountains, plateau, the foot of a mountain and north to the Maine southeast, south to Florida, west to Louis
The area, coastal plain of Si Anna, the distribution of hinterland is the most northern southern to Indiana and Quebec；Have evergreen, cold-resistant,
Strong adaptability, tree-like attractive in appearance, pattern is changeable, flower is of dazzling beauty, it is high with economic worth to view and admire, selected by U.S. health alunite Dick state
For state flower.In order to enrich the variety source of Guizhou Woody flower, promote the process of Guizhou flowers research and industrialized development, from U.S.
State introduced Florida bridesmaid's mountain laurel (K. latifolia ‘Bridesmaid’) seed, and to it in Guizhou
The seed propagation technology saved is studied, and provides seed propagation and introduction and acclimatization skill for mountain laurel in Guizhou or even plantation and popularization
Art supports.This method material bridesmaid's mountain laurel seed, from Fla. import, carries out mountain laurel in Guiyang City, Guizhou Province
Sowing germination test.America bridesmaid's mountain laurel seed need not process especially can be sprouted, but seed germination rate is the highest.Cause
This is necessary to provide a kind of method improving mountain laurel germination.
Summary of the invention
It is an object of the invention to, for solving the problem that after existing skill bridesmaid's mountain laurel weekly is transplanted, seed germination rate is the highest, carry
The method emerged for a kind of bridesmaid's of quickening mountain laurel sowing seedling.
The method that quickening bridesmaid's mountain laurel sowing seedling of the present invention is emerged is achieved through the following technical solutions:
By seed first with the alcohol disinfecting 15-25s of 70-80%, taking-up use distilled water flushing 3～5 times, put into the temperature of 15 DEG C-65 DEG C
Water soaks, after soaking 10-50min, takes out the germination box being placed in one layer of germination paper；Seed-bearing for Sheng germination box is placed in light
According to intensity be 3000xl, light application time be 12h, temperature be that the constant temperature illumination box of 25 DEG C carries out cultivating to seed and sprouting completely
Send out.
Preferably, warm water soaking seed is substituted with the gibberellins that concentration is 100-500mg/L.
It is further preferred that soak seed 15min with gibberellins that concentration is 400 mg/L or be 300 mg/L by concentration
Gibberellins when soaking seed 30min percentage of seedgermination, germinating energy and germination refer to best.
The technique effect of the present invention: technical scheme can make to snow the percentage of seedgermination of mountain laurel from less than 40%
Significantly improve about 80%.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated, and the numerical value related in embodiment or ratio, such as nothing
Special instruction, all represents mass figures or mass ratio.
Embodiment 1:
1, soaking temperature and the soak time process to bridesmaid's mountain laurel seed germination:
Seed first with 75% alcohol disinfecting 20s, take out with distilled water flushing 3～5 times, be respectively put into 20 DEG C, 40 DEG C, 60 DEG C of temperature
In water, (water is sterilized water) soaks, and first squirts profit by being lined with germination paper sterilized water in the germination box of germination paper, respectively at
Taking out after 15min, 30min and 45min and be placed on germination paper cultivation, control treatment is the seed not soaked of only sterilizing.Every box
Germination box about puts about 500 seeds, is repeated 3 times.Seed-bearing for Sheng germination box is placed in intensity of illumination is 3000xl, light application time
For the constant temperature illumination box that 12h, temperature are 25 DEG C.During experiment, add water in good time, keep filter paper to moisten, be found to have mouldy
Seed is rejected in time.Break through seed coat with first endosperm and be considered as seed germination toggle speed, treat after first germination every 2
It records a germination number, to find out the suitableeest soaking temperature and the seed soaking time of bridesmaid's mountain laurel seed germination.Result is shown in
Table 1.
By table 1 it is apparent that bridesmaid's mountain laurel seed of 40 DEG C of warm water soaking, its emergence rate is significantly higher than comparison
And the process of other warm water (CK), seed starts germination physiology and relatively compares (CK) and substantially shortens, and soak time with 15min and
30min is advisable.But the emergence rate of bridesmaid's mountain laurel seed is the highest by only about 40%.
2, the variable concentrations gibberellins seed soaking process to the mountain laurel seed germination that snows:
For improving the emergence rate of bridesmaid's mountain laurel seed further, the gibberellins carrying out variable concentrations with optimum water temperature 40 DEG C soaks
Experimental pretreatment.Seed first with 75% alcohol disinfecting 20s, take out with distilled water flushing 3-5 time, respectively in 40 DEG C of warm water, concentration
It is respectively 100 mg/L, 200 mg/L, 300 mg/L, 400 mg/L, the Gibberellins solution of 500 mg/L and clear water to soak,
After 15min and 30min, taking-up distilled water flushing is totally placed in the germination box of a metafiltration paper.Sample is taked to take at random
Sample, about 500 seeds of each process, it is repeated 3 times.Seed-bearing for Sheng germination box is placed in intensity of illumination when being 3000xl, illumination
Between for 12h, temperature be 25 DEG C, humidity be 80% constant temperature illumination box.During experiment, add water in good time, keep filter paper to moisten,
It is found to have mouldy seed and picks cleaning in time.Break through seed coat with first endosperm and be considered as seed germination toggle speed, treat first
Germination number of every 3 days records after germination, above process is all with 30d for the cycle of germinateing.The results are shown in Table 2 and 3.
By table 2 it is apparent that relatively compare through bridesmaid's mountain laurel seed of gibberellin treatment and substantially shorten seed
Sprouting toggle speed, average shortening 2-3 days, and the seed of gibberellins 100-500mg/L seed soaking, its emergence rate is significantly higher than
Matched group, wherein gibberellins concentration is 400 mg/L percentages of seedgermination and germination refers to the highest.
By table 3 it is apparent that relatively compare through bridesmaid's mountain laurel seed of gibberellin treatment and substantially shorten seed
Sprouting toggle speed, average shortening 1-3 days, and the seed of gibberellins 100-400mg/L seed soaking, its emergence rate is significantly higher than
Matched group, wherein gibberellins concentration is 300 mg/L percentages of seedgermination and germination refers to the highest.
Certainly, being more than the concrete exemplary applications of the present invention, the present invention also has other embodiment, and all employings are equal to
Replace or the technical scheme of equivalent transformation formation, within all falling within protection domain of the presently claimed invention.
Claims (3)
1. accelerate the method that bridesmaid's mountain laurel sowing seedling is emerged for one kind, it is characterised in that: by seed first with the wine of 70-80%
Essence sterilization 15-25s, takes out and uses distilled water flushing 3-5 time, put in the warm water of 15 DEG C-65 DEG C and soak, take after soaking 10-50min
Go out to be placed in the germination box of one layer of germination paper；Seed-bearing for Sheng germination box is placed in intensity of illumination be 3000xl, light application time be
12h, temperature are that the constant temperature illumination box of 25 DEG C carries out cultivating to seed and sprouting completely.
Bridesmaid's mountain laurel of accelerating the most according to claim 1 sows the method that seedling is emerged, it is characterised in that: use concentration
Gibberellins for 100-500mg/L substitutes warm water soaking seed.
Bridesmaid's mountain laurel of accelerating the most according to claim 2 sows the method that seedling is emerged, it is characterised in that: use concentration
It is that the gibberellins of 400 mg/L soaks seed 15min or soaks seed 30min with gibberellins that concentration is 300 mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610456098.0A CN105993283B (en) | 2016-06-22 | 2016-06-22 | Accelerate the method for bridesmaid's mountain laurel sowing seedling emergence |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610456098.0A CN105993283B (en) | 2016-06-22 | 2016-06-22 | Accelerate the method for bridesmaid's mountain laurel sowing seedling emergence |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105993283A true CN105993283A (en) | 2016-10-12 |
| CN105993283B CN105993283B (en) | 2019-05-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610456098.0A Expired - Fee Related CN105993283B (en) | 2016-06-22 | 2016-06-22 | Accelerate the method for bridesmaid's mountain laurel sowing seedling emergence |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1219102A (en) * | 1995-12-22 | 1999-06-09 | 国际壳牌研究有限公司 | Process for propagation and/or selection of plant material |
-
2016
- 2016-06-22 CN CN201610456098.0A patent/CN105993283B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1219102A (en) * | 1995-12-22 | 1999-06-09 | 国际壳牌研究有限公司 | Process for propagation and/or selection of plant material |
Non-Patent Citations (2)
| Title |
|---|
| 周艳 等: "山月桂的播种育苗技术", 《种子》 * |
| 蔡静如 等: "不同浸种处理对4种杜鹃花杂交种子萌发的影响", 《热带作物学报》 * |
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| CN105993283B (en) | 2019-05-07 |
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Granted publication date: 20190507 Termination date: 20200622 |
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