CN105983023A - External traditional Chinese medicine composition and preparation with function of promoting running and absorption of skin for active substances and preparation method thereof - Google Patents
External traditional Chinese medicine composition and preparation with function of promoting running and absorption of skin for active substances and preparation method thereof Download PDFInfo
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- CN105983023A CN105983023A CN201510085515.0A CN201510085515A CN105983023A CN 105983023 A CN105983023 A CN 105983023A CN 201510085515 A CN201510085515 A CN 201510085515A CN 105983023 A CN105983023 A CN 105983023A
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Abstract
The invention discloses an external traditional Chinese medicine composition with a function of promoting running and absorption of skin for active substances. The composition is prepared from, by weight, 2-6 parts of Chinese ligusticum rhizome, 1-4 parts of radix angelicae sinensis, 1-4 parts of salviae miltiorrhizae, 1-3 parts of cortex cinnamomi and 1-3 parts of Ampelopsis megalophylla Diels et Gilg. It is proved through experiments that the composition has a good permeation promoting effect on peptide substances, vitamins, flavonoids and polysaccharide substances. The problems that in the prior art, the permeation promoting effect is slow and durability is poor are solved, it is proved through experiments that the rapid permeation promoting and lasting permeation promoting function can be achieved, and powerful support is provided with skin emergency repair and lasting protection. It can be verified through safety experiments that the composition has a remarkable anti-allergy and anti-irritation function and is safer and promotes permeation mildly and selectively compared with the prior art. The raw materials are easy to obtain, and the composition is simple in preparation method, green and safe, can be easily accepted by allergic people and has good application prospects and market prospects.
Description
Technical field
The present invention relates to a kind of external medicine composition, specifically one and there is promotion skin active material operation suction
The external medicine composition receiving effect and the skin care formulation prepared by this external medicine composition.
Background technology
Skin care item mainly intersperse among human skin in the way of smearing, spraying, to play cleaning, maintenance, U.S.
The effect held.Additionally, due to the physiological structure of keratodermatitis and function determine, it has barrier exogenous molecule
Enter internal characteristic.This can play the effect of positive aspects resisting exogenous material invasion and attack.Meanwhile, by force
Big skin barrier effect hinders the entrance of the exogeneous reactivity material promoting skin health the most simultaneously.So that skin
Skin obtains more active substance, promotes that active component transdermal operation mechanism of absorption and method become and grind in research skin care item
One of focus studying carefully focus.
At present, there are different types of cutaneous permeable agent to demonstrate reversibly overcoming the potentiality of skin barrier, thus helped
Active component is helped to be carried through skin.Although some is during chemically and physically skin penetrant has been applied to skin care item formula,
But active substance not only is promoted to penetrate into skin, the most simultaneously by harmful substance and stimulus object by some skin Percutaneous absorption enhancers
Matter is brought in skin together, makes skin the infringements such as allergy occur, such as azone.Although some penetration enhancer can play safety
Promote effect, but to promote saturating quick-acting, persistency the best for it.
Therefore, benefit materials, for promoting thoroughly with selectivity, is i.e. only promoted thoroughly to absorb to skin, blocking harmful thing by market
The entrance of matter, thus safety promotees saturating penetration enhancer and has urgent demand.How to increase simultaneously penetration enhancer quick-acting and
Persistency is still the focus of those skilled in the art's research.
Summary of the invention
The primary and foremost purpose of the present invention is to propose a kind of exploitation from natural plants, to promote that skin is transported for active substance
The Chinese medicine composition that row absorbs.
Another object of the present invention is to propose the preparation method of a kind of above-mentioned Chinese medicine composition.
The invention thinking of the present invention is:
The present invention utilizes the biotechnology of advanced person, in conjunction with the theory of Chinese medical science of Chinese tradition, by Rhizoma Ligustici, Radix Angelicae Sinensis, Radix Salviae Miltiorrhizae,
Cortex Cinnamomi, Ampelopsis grossedentata are effectively combined in together, form compound recipe, and obtain extract, by its hydrophilic parent after being extracted
Oiliness, the infiltration lane of formation are easy to active substance and are entered the lipid bilayer of keratodermatitis lipoid, and by it
Antagonism to pungent, reduces the zest to skin and toxicity hidden danger, reaches comprehensive active substance pair
The safe penetration effect of skin.Help better with Herba Menthae and Fructus Corni.
Rhizoma Ligustici: acrid in the mouth, warm in nature.Return bladder warp.There is expelling wind and cold, effect of removing damp and stopping pain.Cure mainly exterior syndrome due to wind-cold,
Top, mountain peak pain, rheumatic arthralgia.
Radix Angelicae Sinensis: nature and flavor are sweet, pungent, warm.Return liver, the heart, spleen channel.Have and enrich blood；Invigorate blood circulation；Menstruction regulating and pain relieving；Moisturize cunning
Effect of intestinal.
Radix Salviae Miltiorrhizae: nature and flavor are bitter, are slightly cold.GUIXIN, Liver Channel.There are stasis-dispelling and pain-killing, promoting blood circulation to restore menstrual flow, the effect of the relieving restlessness that clears away heart-fire.
For menoxenia, amenorrhea dysmenorrhea, lump in the abdomen, chest and abdomen twinge, pyretic arthralgia pain, skin infection swells and ache, dysphoria and insomnia；
Hepatosplenomegaly, angina pectoris.
Cortex Cinnamomi: acrid in the mouth, sweet, extremely hot in nature.Return kidney, spleen, the heart, Liver Channel.There is benefit fire supporing yang, guiding fire to origin, cold expelling
Pain relieving, effect of promoting the flow of QI-blood by warming the meridian.For sexual impotence cold womb, chills and pain of the waist and kness, suffer from a deficiency of the kidney and breathe heavily, upward floating of YANG in deficiency condition, dizziness conjunctival congestion,
Trusted subordinate's cold type of pain, deficiency and coldness vomiting and diarrhoea, colic of cold type stomachache, dysmenorrhea amenorrhea.
Ampelopsis grossedentata: be commonly called as rattan mother-in-law's tea, Ampelopsis grossedentata, white monkey, mountain Folium hydrangeae strigosae, dragon beard tea, Tujia's manna or Tujia's god's tea etc., its
Formal name used at school is Ampelopsis grossedentata [Ampelopsis grossedentata (Hand-Mazz) W.T.wang].Main distribution
In China Hunan, Hubei, Guangxi and other places, can be not only used for treating laryngopharynx swelling and pain, cold, fever, icterohepatitis, bleb
The diseases such as furuncle, can drink by daily bubble again, cool, thirst-quenching.In Ampelopsis grossedentata, flavones content high (up to more than 40%) is in plant kingdom
In really belong to exception, polyphenol content the highest (about 20%), flavone compound and polyphenol compound have
Physiologically active widely.Modern medicine research shows, Ampelopsis grossedentata and extract oral thereof have regulation immunity, antitumor,
Antioxidation, blood fat reducing, blood pressure lowering, blood sugar lowering, protect the liver, hepatoprotective and alleviate the effects such as ethylism.
Herba Menthae: acrid in the mouth, cool in nature.Enter lung, Liver Channel.There is dispelling wind and heat pathogens, the clear profit head, sore-throat relieving rash, soothing the liver row
Effect of gas.Cure mainly affection due to external wind and heat, headache, laryngopharynx swelling and pain, dyspepsia flatulence, aphtha, toothache, skin ulcer scabies, urticaria,
Epidemic febrile disease is from the beginning of, rubella pruritus, stagnation of QI due to depression of the liver, hypochondriac pain uncomfortable in chest.
Fructus Corni: another name Fructus Corni, Fructus Corni, mountain are in meat etc., and mature fruit is Chinese medicine, for vertigo and tinnitus, waist
Knee joint soreness, impotence and seminal emission, enuresis frequent micturition, bleeding not during menses, profuse sweating is collapsed.
For realizing the purpose of the present invention, the present invention adopts the following technical scheme that
A kind of have the external medicine composition promoting that skin active material runs absorption effect, by following weight parts proportioning
Crude drug make:
Rhizoma Ligustici 2～6 weight portion, Radix Angelicae Sinensis 1～4 weight portion, Radix Salviae Miltiorrhizae 1～4 weight portion, Cortex Cinnamomi 1～3 weight portion, rattan
Tea 1～3 weight portion.
A kind of have the external medicine composition promoting that skin active material runs absorption effect, by following weight parts proportioning
Crude drug make:
Rhizoma Ligustici 2～6 weight portion, Radix Angelicae Sinensis 1～4 weight portion, Radix Salviae Miltiorrhizae 1～4 weight portion, Cortex Cinnamomi 1～3 weight portion, rattan
Tea 1～3 weight portion, Herba Menthae 1～3 weight portion, Fructus Corni 1～3 weight portion.
The preparation method of a kind of above two external medicine composition, specifically comprises the following steps that
(1) weigh by described weight after above-mentioned raw materials medicated powder is broken, mixing；
(2) percent by volume is 60%～90% ethanol extraction, crude drug and ethanol mass volume ratio g/ml be 1:10～
1:30,70 DEG C～90 DEG C are extracted 1～4 hour；
(3) extracting solution that step (2) obtains is cooled to 20 DEG C～30 DEG C, 200～400 mesh coarse filtrations, leach
Carry out vacuum filtration after medicinal residues, collect secondary filtrate；
(4) whole for filtrate rotary evaporation ethanol are obtained concentrated solution, concentrated solution adds 1,3 butylene glycol, addition
For step (3) gained secondary amount of filtrate percent by volume 60%～90%；Miscible uniformly, filter, take clear liquid, i.e.
?.
In described step (2), the percent by volume of ethanol is 70%, and crude drug and ethanol mass volume ratio g/ml are 1:
15；Extraction time is 3 hours.
In described step (3), vacuum filtration condition is paving two layers of filter paper in buchner funnel, in the middle of filter paper folder 0.3～
0.6cm kieselguhr carries out vacuum filtration.
Described step (4) is at vacuum-0.09MPa, the whole ethanol of rotary evaporation under the conditions of 40 DEG C～50 DEG C.
A kind of have the external application Chinese medicine extract promoting that skin active material runs absorption effect, wherein said external application Chinese medicine
Extract is obtained by said method extraction.
Above-mentioned external application Chinese medicine extract has the skin care item interpolation promoting that skin active material runs absorption effect in preparation
Purposes in agent.
A kind of have promote skin active material run absorb effect skin care product additive, described skin care product additive by
Prepare after above-mentioned external application Chinese medicine extract is sterilized.Described sterilizing program is 90 DEG C of water-bath sterilizations 20～40 minutes.
The Chinese medicine extract that the present invention provides can be added in skin care item, to promote skin care item in skin care item preparation process
Middle active substance is absorbed by the skin.This Chinese medicine composition is smeared skin after being simply pulverized and mixed and be may also function as certain rush
Effect thoroughly.
The beneficial outcomes of the present invention:
The present invention is verified by experiments short peptide matters, vitamins, flavonoid and active components of plants polysaccharose substance
All have and well promote saturating effect.Through safety experiment it can be confirmed that the present invention has obvious antiallergic irritation effect,
Selectivity safer than prior art, gentle promotees thoroughly.Next instant invention overcomes and promotees effect in prior art slowly,
The problem of persistency difference, the present invention is verified by experiments and can reach effect that the saturating and long-acting rush of quick-acting rush is saturating, should to skin
Anxious reparation and persistently protection provide strong support.Raw material of the present invention obtains easily, preparation method is easy, green peace
Entirely, it is easy to accepted by allergic human population, there is good application prospect and market prospect.
Accompanying drawing explanation
Fig. 1 is for promoting Argireline fluorescent tracing tissue slice figure (upper left: embodiment 3；Upper right: embodiment 2；Lower-left:
Embodiment 1；Bottom right: 1% azone)；
Fig. 2 is niacinamide content standard curve；
Fig. 3 is that variable concentrations embodiment 1 extract promotees nicotiamide absorption transmittance curve；
Fig. 4 is that to the tissue slice of guinea pig skin, (upper behavior variable concentrations azone processes HE tissue to embodiment 1 with azone
Section, lower behavior variable concentrations embodiment extract-treated HE tissue slice figure)；
Fig. 5 is different embodiment saturating effects of rush to 1% trehalose；
Fig. 6 is different embodiment saturating effects of rush to 10% oat polypeptide；
Fig. 7 is different penetration enhancer saturating effects of rush to 5% licoflavone.
Detailed description of the invention
Medical material used in the present invention all can be commercially available from China Medicament Group Corp., meets the People's Republic of China (PRC)
2005 editions standards of pharmacopeia.Other raw materials all can be obtained by commercially available purchase, and instrument title and producer used by the present invention see
Table 1.
Table 1
| Title | Model | Producer |
| Thermostat water bath | HH·S1-M | Chang'an, Beijing scientific instrument factory |
| High-speed multifunctional pulverizer | JP-400B-8 | Yongkang City nine grades of rank in the feudal regimes Trade Co., Ltd. |
| Intelligent temperature control heating stirrer | SZCL | Yu Hua Instrument Ltd. of Gongyi City |
| Rotary evaporator | RE-2000 | Shanghai Yarong Biochemical Instrument Plant |
| Circulating water type vacuum pump | SHB-Ⅲ | Zhengzhou Greatwall Scientific Industrial & Trading Co., Ltd. |
The preparation of embodiment 1 Chinese medicine composition of the present invention
(1) following raw material medicaments is weighed by described weight proportion after pulverizing, mixing；
Rhizoma Ligustici 20g, Radix Angelicae Sinensis 10g, Radix Salviae Miltiorrhizae 10g, Cortex Cinnamomi 10g, Ampelopsis grossedentata 20g, Herba Menthae 10g, Fructus Corni 10g.
(2) percent by volume is 70% ethanol extraction, and crude drug and ethanol mass volume ratio g/ml are 1:15, put
Being placed in electric-heated thermostatic water bath, 80 DEG C are extracted 3 hours；
(3) extracting solution that step (2) obtains is taken out from thermostat water bath, stand and be cooled to room temperature, 200 mesh
Coarse filtration, leaches medicinal residues, obtains filtrate；This filtrate is carried out again vacuum filtration, sucking filtration condition: spread in buchner funnel
Two layers of filter paper, folder 0.5cm kieselguhr vacuum filtration in the middle of filter paper, collect secondary filtrate；
(4) secondary filtrate is at vacuum-0.09MPa, and under the conditions of 43 DEG C, the whole ethanol of rotary evaporation obtains concentrated solution；Add
The 1,3 butylene glycol of secondary amount of filtrate 70% (percent by volume) is miscible uniformly with concentrated solution, filters, takes clear liquid, i.e.
?.
The preparation of embodiment 2 Chinese medicine composition of the present invention
(1) following raw material medicaments is weighed by described weight proportion after pulverizing, mixing；
Rhizoma Ligustici 60g, Radix Angelicae Sinensis 20g, Radix Salviae Miltiorrhizae 30g, Cortex Cinnamomi 20g, Ampelopsis grossedentata 10g, Herba Menthae 20g, Fructus Corni 30g
(2) percent by volume is 90% ethanol extraction, and crude drug and ethanol mass volume ratio g/ml are 1:10, put
Being placed in electric-heated thermostatic water bath, 70 DEG C are extracted 4 hours；
(3) extracting solution that step (2) obtains is taken out from thermostat water bath, stand and be cooled to 20 DEG C, 200 mesh
Coarse filtration, leaches medicinal residues, obtains filtrate；This filtrate is carried out again vacuum filtration, sucking filtration condition: spread in buchner funnel
Two layers of filter paper, folder 0.3cm kieselguhr vacuum filtration in the middle of filter paper, collect secondary filtrate；
(4) secondary filtrate is at vacuum-0.09MPa, and under the conditions of 40 DEG C, the whole ethanol of rotary evaporation obtains concentrated solution；Add
The 1,3 butylene glycol entering secondary amount of filtrate 90% (percent by volume) is miscible uniformly with concentrated solution, filters, takes clear liquid,
Obtain.
The preparation of embodiment 3 Chinese medicine composition of the present invention
(1) following raw material medicaments is weighed by described weight proportion after pulverizing, mixing；
Rhizoma Ligustici 30g, Radix Angelicae Sinensis 40g, Radix Salviae Miltiorrhizae 40g, Cortex Cinnamomi 30g, Ampelopsis grossedentata 30g, Herba Menthae 30g, Fructus Corni 20g
(2) percent by volume is 60% ethanol extraction, and crude drug and ethanol mass volume ratio g/ml are 1:30, put
Being placed in electric-heated thermostatic water bath, 90 DEG C are extracted 1 hour；
(3) extracting solution that step (2) obtains is taken out from thermostat water bath, stand and be cooled to 30 DEG C, 400 mesh
Coarse filtration, leaches medicinal residues, obtains filtrate；This filtrate is carried out again vacuum filtration, sucking filtration condition: spread in buchner funnel
Two layers of filter paper, folder 0.6cm kieselguhr vacuum filtration in the middle of filter paper, collect secondary filtrate；
(4) secondary filtrate is at vacuum-0.09MPa, and under the conditions of 50 DEG C, the whole ethanol of rotary evaporation obtains concentrated solution；Add
The 1,3 butylene glycol entering secondary amount of filtrate 60% (percent by volume) is miscible uniformly with concentrated solution, filters, takes clear liquid,
Obtain.
The preparation of embodiment 4 Chinese medicine composition of the present invention
(1) following raw material medicaments is weighed by described weight proportion after pulverizing, mixing；
Rhizoma Ligustici 20g, Radix Angelicae Sinensis 10g, Radix Salviae Miltiorrhizae 10g, Cortex Cinnamomi 10g, Ampelopsis grossedentata 20g.
(2) percent by volume is 70% ethanol extraction, and crude drug and ethanol mass volume ratio g/ml are 1:15, put
Being placed in electric-heated thermostatic water bath, 80 DEG C are extracted 3 hours；
(3) extracting solution that step (2) obtains is taken out from thermostat water bath, stand and be cooled to room temperature, 200 mesh
Coarse filtration, leaches medicinal residues, obtains filtrate；This filtrate is carried out again vacuum filtration, sucking filtration condition: spread in buchner funnel
Two layers of filter paper, folder 0.5cm kieselguhr vacuum filtration in the middle of filter paper, collect secondary filtrate；
(4) secondary filtrate is at vacuum-0.09MPa, and under the conditions of 43 DEG C, the whole ethanol of rotary evaporation obtains concentrated solution；Add
The 1,3 butylene glycol of secondary amount of filtrate 70% (percent by volume) is miscible uniformly with concentrated solution, filters, takes clear liquid, i.e.
?.
The preparation of embodiment 5 Chinese medicine composition of the present invention
(1) following raw material medicaments is weighed by described weight proportion after pulverizing, mixing；
Rhizoma Ligustici 60g, Radix Angelicae Sinensis 20g, Radix Salviae Miltiorrhizae 30g, Cortex Cinnamomi 20g, Ampelopsis grossedentata 10g.
(2) percent by volume is 90% ethanol extraction, and crude drug and ethanol mass volume ratio g/ml are 1:10, put
Being placed in electric-heated thermostatic water bath, 70 DEG C are extracted 4 hours；
(3) extracting solution that step (2) obtains is taken out from thermostat water bath, stand and be cooled to 20 DEG C, 200 mesh
Coarse filtration, leaches medicinal residues, obtains filtrate；This filtrate is carried out again vacuum filtration, sucking filtration condition: spread in buchner funnel
Two layers of filter paper, folder 0.3cm kieselguhr vacuum filtration in the middle of filter paper, collect secondary filtrate；
(4) secondary filtrate is at vacuum-0.09MPa, and under the conditions of 40 DEG C, the whole ethanol of rotary evaporation obtains concentrated solution；Add
The 1,3 butylene glycol entering secondary amount of filtrate 90% (percent by volume) is miscible uniformly with concentrated solution, filters, takes clear liquid,
Obtain.
The preparation of embodiment 6 Chinese medicine composition of the present invention
(1) following raw material medicaments is weighed by described weight proportion after pulverizing, mixing；
Rhizoma Ligustici 30g, Radix Angelicae Sinensis 40g, Radix Salviae Miltiorrhizae 40g, Cortex Cinnamomi 30g, Ampelopsis grossedentata 30g.
(2) percent by volume is 60% ethanol extraction, and crude drug and ethanol mass volume ratio g/ml are 1:30, put
Being placed in electric-heated thermostatic water bath, 90 DEG C are extracted 1 hour；
(3) extracting solution that step (2) obtains is taken out from thermostat water bath, stand and be cooled to 30 DEG C, 400 mesh
Coarse filtration, leaches medicinal residues, obtains filtrate；This filtrate is carried out again vacuum filtration, sucking filtration condition: spread in buchner funnel
Two layers of filter paper, folder 0.6cm kieselguhr vacuum filtration in the middle of filter paper, collect secondary filtrate；
(4) secondary filtrate is at vacuum-0.09MPa, and under the conditions of 50 DEG C, the whole ethanol of rotary evaporation obtains concentrated solution；Add
The 1,3 butylene glycol entering secondary amount of filtrate 60% (percent by volume) is miscible uniformly with concentrated solution, filters, takes clear liquid,
Obtain.
The preparation of embodiment 7 skin care product additive of the present invention
Skin care product additive is sterilized rear prepared by embodiment 1 Chinese medicine extract.Described sterilizing program is that 90 DEG C of water-baths are gone out
Bacterium 20 minutes.
The preparation of embodiment 8 skin care product additive of the present invention
Skin care product additive is sterilized rear prepared by embodiment 1 Chinese medicine extract.Described sterilizing program is that 90 DEG C of water-baths are gone out
Bacterium 40 minutes.
Efficacy experiments of the present invention
One, Argireline transdermal is run and inhales by embodiment 1, embodiment 2, embodiment 3 gained Chinese medicine composition A, B, C
Receive efficacy assessments
(1) research purpose
By observing Chinese medicine composition A, B, C impact on Corium Mus permeability, evaluate drug composition A, B, C to small peptide
Class active substance transdermal runs the positive role absorbed.
(2) experiment material
By test product: embodiment 1, embodiment 2, embodiment 3, gained Chinese medicine composition A, B, C positive control 1% azone
Laboratory animal: remove subcutaneous fat and the Cavia porcellus Corium Mus of blood vessel, (cultivating company purchased from Beijing gold shepherd laboratory animal).
Reagent: Argireline (Beijing Ze Xiyuan company), cyanine dyes (CyDye3, the general rich biochemistry in Beijing), 0%, 20%, 30%
Sucrose PBS (autogamy)
Experimental apparatus: skin biopsy machine (Leica CM1850), laser confocal fluorescence microscope (LSCM-TCS-SP5,
Leica), pipettor
(3) experimental technique
First quality purity Argireline more than 98% will be obtained, with cyanine dyes expection molecule linkage flag.Choose
200-300g weight Cavia porcellus incubated overnight, is divided into A, B bis-groups, is lost hair or feathers at its back respectively.Afterwards the most plucked Cavia porcellus
On back, one radius of labelling is the border circular areas of 1cm, and it is consistent to be respectively labeled as also position holding；For A group Cavia porcellus,
Respectively it is smeared different Chinese medicine extract, for B group Cavia porcellus, the most respectively it is smeared azone, exist the most respectively
All guinea pig backs smear the composition Argireline containing fluorescent marker.After 0.5 hour, the most de-neck puts to death A, B
Two groups of Cavia porcelluss, its labelling area skin of clip, carefully peel off, make frozen section, nial polish mounting.
Tissue slice key step:
A. the Mus after marked transdermal is put to death rapidly, take the Corium Mus of its marked region.
It is positioned over the most rapidly 4% paraformaldehyde and fixes about 5 hours.
C. using 0%, 20% after removing, 30% sucrose PBS sinks to the bottom successively, the most about 1 hour time.
D. quick freeze after taking out, is placed in freezing microtome, 20 μm sections.
E. it is placed in the microscope slide paster that poly-D-lysine is washed, dries.
F. confocal microscope (laser instrument is He-Ne 633nm) observation structure.
Finally, the frozen section through making is placed under laser confocal fluorescence microscope observation photographic analysis result.
(4) experimental result
Embodiment 3, has had certain fluorescence signal, i.e. Argireline to have in guinea pig skin certain saturating in the skin of Cavia porcellus
Cross.
Embodiment 2, has had strong fluorescence signal in the skin of Cavia porcellus, and existing the most accumulative in the dermis, i.e. six victory
Peptide has more passing through in guinea pig skin.
Embodiment 1, fluorescence signal has Dispersed precipitate in the skin of Cavia porcellus, it may be possible to Argireline had in guinea pig skin
Degree has penetrated in subcutaneous tissue.
Azone under 1.0% concentration, has had strong fluorescence signal in the skin of Cavia porcellus, and i.e. Argireline is in guinea pig skin
Have and more penetrate in subcutaneous tissue, be specifically shown in Fig. 1.
The extract that inventor utilizes embodiment 4,5,6 to prepare repeats above-mentioned experiment, and its result all shows extract
Have permeability to Argireline, experiment effect is with above-mentioned.
One of most important component of human body skin is exactly protein, and protein is exactly the victory peptide chain that aminoacid bond becomes
(poly-peptide).The great majority used in the skin care item that some skin is relevant at present are no more than 10 amino
Acid small peptide such as: two victory peptides, palmitoyl tripeptide 3, Wushengtai, Argireline etc..Argireline is in cosmetics victory peptides additive
It is the bigger molecular weight of molecular weight ratio (888), because our experiment demonstrates the transporting factor and Argireline can be had rush
Effect thoroughly, so other small peptides that are less for other molecular weight and that be often added in cosmetics also have transdermal enhancing effect.
Two, embodiment 1 Chinese medicine extract A is to vitamin substances transdermal enhancing effect
1. experiment purpose:
Vitamin substances has good nutritive effect for skin, is to maintain the necessary class activity of human life activity
Material, has whitening, the multiple efficacies such as anti-ageing to skin, is conventional functional additive in cosmetics.By vitamins
The more efficient rush of material penetrates into skin, beneficially skin health, it is possible to reduce the content of effect material in cosmetics, fall
Low production cost.So the research carrying out vitamin substances transdermal enhancing effect has become as grinding of cosmetic industry in recent years
Study carefully one of focus.
2. experiment content
2.1 instruments and reagent
2.1.1 instrument: high performance liquid chromatograph；Franz Transdermal absorption instrument；Analytical balance；Ultrasound Instrument；Pipet；Capacity
Bottle；Turbula shaker, Corium Mus (Beijing gold shepherd laboratory animal cultivates company).
2.1.2 reagent nicotiamide reference substance (being purchased from Guangzhou Nansha Lonza Co., Ltd.)；Normal saline (is purchased from Cologne, Sichuan
Pharmaceutical limited company, lot number M13072421)；Water-solubleazone, (is purchased from Beijing this biochemistry of Berry Lay to have
Limit company)；Sample detail is shown in Table 2.
Table 2 sample is detailed
2.1.3 sample preparation methods
Sample 1: accurately weigh nicotiamide reference substance 0.300g, embodiment 1 Chinese medicine extract A0.030g, normal saline
5.670g, fully dissolves, and is prepared as the nicotiamide that mass fraction is 5%, and the embodiment 1 Chinese medicine extract A of 0.5% supplies
Feeding.4 DEG C of preservations are stand-by, recover room temperature before using.
Sample 2: accurately weigh nicotiamide reference substance 0.300g, embodiment 1 Chinese medicine extract A0.060g, normal saline
5.640g, fully dissolves, and is prepared as the nicotiamide that mass fraction is 5%, and the embodiment 1 Chinese medicine extract A of 1.0% supplies
Feeding.4 DEG C of preservations are stand-by, recover room temperature before using.
Sample 3: accurately weigh nicotiamide reference substance 0.300g, embodiment 1 Chinese medicine composition A0.120g, normal saline
5.580g, fully dissolves, and is prepared as the nicotiamide that mass fraction is 5%, and the embodiment 1 Chinese medicine composition A of 2.0% supplies
Feeding.4 DEG C of preservations are stand-by, recover room temperature before using.
Sample 4: accurately weigh nicotiamide reference substance 0.300g, water-solubleazone 0.060g, normal saline 5.640g, fully
Dissolving, be prepared as the nicotiamide that mass fraction is 5%, the water-solubleazone of 1.0% is for feeding.4 DEG C of preservations are stand-by, make
By front recovery room temperature.
Sample 5: accurately weigh nicotiamide reference substance 0.300g, normal saline 5.700g, fully dissolve, be prepared as quality and divide
Number is that the nicotiamide of 5% is for feeding.4 DEG C of preservations are stand-by, recover room temperature before using.
Prepared by 2.2 Corium Mus:
2.2.1 de-for Cavia porcellus (Beijing Military Medical Academy animal center) cervical vertebra is put to death；
2.2.2 rapidly its belly wool medical scissors is cut short as far as possible；
2.2.3 with depilatory cream, its belly wool is purified；
2.2.4 peel off skin of abdomen and remove subcutaneous fat, blood vessel, rinses to only repeatedly with distilled water；
2.2.5 with normal saline flushing several times, cold preservation in-80 DEG C of refrigerators is put standby.
2.3 Franz diffusion cells carry out Transdermal Absorption experiment
2.3.1 Corium Mus: before experiment starts, is placed in normal saline immersion 15min by Corium Mus.
2.3.2 acceptable solution: normal saline.
2.3.3 for feeding: A, B, C, D, E.
2.3.4 experimentation:
Suitable quantity of water is added in body outer osmotic disperser temperature chamber.Power-on and temperature chamber magnetic agitation, set perseverance
In temperature groove, water temperature is 37 scholar 0.1 DEG C.Pellosil agrafe is fixed between two Room diffusion cells, to vertical proliferation pond
Acceptance pool in add 12ml acceptable solution, put in body outer osmotic disperser temperature chamber preheat, set acceptance pool stirring
Speed is 400 revs/min.It is separately added in supply pool for feeding 5ml.Start to clock, when sample permeate 0.5h, 1h,
2h, 3h, 4h, 6h, 10h sample 400 μ L and put in tool plug centrifuge tube, supplement while every sub-sampling in acceptance pool
The acceptable solution of equivalent also gets rid of the gas in pond.
3. experimental result
3.1 sample pre-treatments
Precision weighs sample 100 μ L, add flowing phase constant volume to 1mL, add flow phase dilution to scale, shake up.Take one
Determining solution and cross 0.45 μm organic system filter membrane, filtrate is stored in 2mL brown sample injection bottle as treating sample measuring liquid, standby.
Prepared by 3.2 titers
Precision weighs nicotiamide standard substance 1mg and is placed in the volumetric flask of 10mL, adds flowing phased soln and is diluted to scale,
Shake up, as storing solution.Precision weighing storing solution 0.2mL, 0.4mL, 0.6mL, 0.8mL, 1.0mL are in tool plug examination
Guan Zhong, constant volume to 1mL, make the solution of 20,40,60,80,100 μ g/mL.Take certain solution and cross 0.45 μm
Organic system filter membrane, filtrate is stored in 2mL brown sample injection bottle as treating sample measuring liquid, standby.
3.3 chromatographic condition
Chromatographic column, C18 chromatographic column (250mm × 4.6mm, 5 μm)；
Flowing phase: methanol-water (20:80)；
Flow velocity: 1.0ml/min；
Detection wavelength is: 261nm；
Sampling volume: 20 μ l；
Column temperature: 30 DEG C；
Theoretical cam curve is calculated by nicotiamide peak and is not less than 3000.
Prepared by 3.4 standard curves
Taking standard working solution sample introduction respectively, with target peak area as vertical coordinate, titer solution concentration is that abscissa enters
Line linearity returns, Criterion working curve.It is specifically shown in Fig. 2.
Table 3 standard curve test result
3.5 sample size test results
Take the solution sample introduction to be measured processed under " 3.1 ", according to the peak area of mensuration composition, substitute into standard work song respectively
On line, obtain the quality solubility of nicotiamide.
The test result of table 4 sample A
The test result of table 5 sample B
The test result of table 6 sample C
The test result of table 7 sample D
The test result of table 8 sample E
3.6 data acquisition
Percutaneous permeability Q (μ g/cm is calculated according to below equation2)。
Note: Q is Percutaneous permeability (μ g/cm2), S is transdermal diffusion area (cm2), V1For Franz diffusion cell acceptable solution
Volume (mL), the mass concentration (μ g/mL) of acceptable solution when ρ n is n-th sampling, when ρ i is i & lt sampling
The mass concentration (μ g/mL) of acceptable solution, V2For sampling amount (mL).
Table 9 sample A through cumulant
Table 10 sample B through cumulant
Table 11 sample C through cumulant
Table 12 sample D through cumulant
Table 13 sample E through cumulant
It is specifically shown in Fig. 3.
3.7 analyze and discuss
Experimental data shows, 10h concentration is that the Percutaneous permeability of the unit are of 5% nicotinamide soln self is only
3577.0μg/cm2, after using embodiment 1 to carry out promoting thoroughly, Percutaneous permeability increases.Embodiment 1 is to nicotinoyl
It is 1.0% that amine promotees saturating onset amount.Promoting 0.5h, unit are Percutaneous permeability increases by 145%；Promote 4h, unit plane
Long-pending Percutaneous permeability increases by 109%；Promoting 10h, unit are Percutaneous permeability increases by 109%.Embodiment 1 is to nicotinoyl
The saturating effect of rush of amine, increases along with the increase of embodiment 1 concentration.The embodiment 1 of 2.0% promotees 4h, unit are
Percutaneous permeability increases by 182%；Promoting 10h, unit are Percutaneous permeability increases by 216%.
Inventor repeats above-mentioned experiment with the Chinese medicine extract that embodiment 2 to 6 obtains, and its result all shows extract pair
Nicotiamide is had and well promotees saturating effect.
Vitamin can be divided into fat-soluble (such as vitamin A, vitamin D, vitamin E, vitamin K) according to dissolubility
With water solublity (such as vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B12, vitamin C) two
Big class.Fatsoluble vitamin has similarity with the lipid material structure in skin barrier, has the similar characteristic mixed,
Dissolubility is preferable.Water soluble vitamins, according to the similar principle that mixes, transdermal has certain obstruction.Nicotiamide is water solublity
Vitamin representative species B3Precursor, has excellent whitening effect.Nicotiamide is had very well by Chinese medicine extract of the present invention
The saturating effect of rush, according to similar same principle, illustrate that it is respectively provided with the saturating effect of good rush to water soluble vitamins.
Three, embodiment 1 gained Chinese medicine extract A safety in utilization is evaluated
Detect whether researched and developed embodiment 1 has good safety or zest, in the condition with or without embodiment 1
Under live body guinea pig skin is processed, and make classics HE tissue paraffin section de observe.By comparing Cavia porcellus skin
The difference of skin epidermis injury just can check safety or the zest of penetration enhancer.
Experiment material
Skin biopsy machine, HE dyestuff, Lycra fluorescence microscope, pipettor,
Label: embodiment 1 extract, azone, Yihong, hematoxylin, dimethylbenzene, paraffin etc.
Main flow
(1) sample 1 (embodiment 1) and the sample 2 (nitrogen of variable concentrations (0%, 0.5%, 1.0%, 2.0%) are first prepared
Ketone).
(2) choose 200～300g weight Cavia porcelluss, be divided into A, B bis-groups, lost hair or feathers in its back respectively.Afterwards the most plucked
On guinea pig back, three radiuses of labelling are the border circular areas of 1cm, and it is consistent to be respectively labeled as also position holding.
(3) for A group Cavia porcellus, respectively it is smeared the sample 1 (example 1) of variable concentrations, for B group Cavia porcellus, then
Respectively it is smeared the sample 2 (azone) of variable concentrations.
(4) after 0.5 hour, the most de-neck puts to death bis-groups of Cavia porcelluss of A, B, its labelling area skin of clip, carefully shells
From, make HE section.
HE cuts into slices key step
A () section dewaxes 5～10 minutes in dimethylbenzene.
B () moves in dimethylbenzene and absolute alcohol (1: 1) mixed liquor about 5 minutes (as dewaxed through secondary dimethylbenzene, this walks
Can omit).
C () enters 100%, 95%, 85%, 70% ethanol, at different levels is 2～5 minutes.Dye liquor is proceeded to through distilled water after.
D () hematoxylin dye liquor dyes 5～15 minutes.
(e) washing slide on unnecessary dye liquor, 0.5～1% hydrochloride alcohol (70% alcohol) color separation a moment.Microscopy controls,
Till in nucleus and core, chromatin is clear, approximate number 10 seconds.
(f) flowing water rinse 15～30 minutes, or in lithium carbonate saturated solution the short time alkalization or oil blackeite, i.e. nucleus in
Blue.
G () distilled water is short washes.
H () 0.1～0.5% eosin stain dye 1～5 minute, if coloring difficulty, can add in every 100 milliliters of dye liquors
1～2 glacial acetic acid, makes easy coloring and is difficult to decolouring.
I (), successively through 70%, 85%, 95%, 100% dehydration of alcohol, at different levels is 2～3 minutes, dense below 95%
In the ethanol of degree, easily decolour in Yihong, should suitably shorten the time.
(j) dimethylbenzene transparent (secondary), about 10 minutes altogether.
(k) mounting: wipe section unnecessary dimethylbenzene around, be sure not to dry up, drip rapidly appropriate neutral gum, then add a cover glass
Sheet sealing.
Experimental result: as shown in Figure 4.
Embodiment 1 extract: embodiment 1 extract is under the concentration of 0%, in the presence of i.e. there is no the transporting factor, guinea pig skin
In epidermal area especially horny layer the most intact；Embodiment 1 extract is under the concentration of 0.5%, at the epidermis of Cavia porcellus
Layer is the most intact, and only regional area starts fold or thinning occur；Embodiment 1 extract 1.0% dense
Under degree, guinea epidermis has thinning, and locally horny layer starts to go out in conjunction with shakiness with epidermis or come off；Embodiment 1 carries
Taking thing under the concentration of 2.0%, there is the thinning phenomenon of dispersivity horny layer in guinea epidermis layer.
Azone: azone is under the concentration of 0%, in the presence of i.e. not having azone, the especially horny layer of the epidermal area in guinea pig skin is non-
The most intact；Azone is under the concentration of 0.5%, and the epidermal area Cavia porcellus starts large area fold occur, horny layer start with
Epidermis goes out in conjunction with steady even segregation phenomenon；Azone is under the concentration of 1.0%, and epidermal area and horny layer Cavia porcellus start
Occurring that large area comes off, fold, epidermal area is impaired substantially；Azone is under the concentration of 2.0%, at the epidermal area pleat of Cavia porcellus
Wrinkle, impaired substantially.
Conclusion
According to experimental result, from longitudinal direction (concentration): embodiment 1 concentration is used below 2%, to guinea epidermis
Skin, especially cuticle loss are smaller, thus embodiment 1 safe concentration is: ＜ 2%；And azone concentration
When 0.5%, guinea epidermis horny layer is just with the presence of segregation phenomenon, and when 1%, horny layer has the existing picture that comes off, and when 2%, epidermis is subject to
Damage serious, thus the safe concentration of azone is: ＜ 1%.From laterally (comparing with same concentration azone): same concentration
Under the stimulation the most cuticular for guinea epidermis of (0.5%, 1.0%, 2.0%) azone or injury be all significantly greater than real
Execute example 1.To sum up, embodiment 1 extract has obvious antiallergic irritation effect, and gentle rush is saturating, safety in utilization
It is significantly better than azone.Inventor repeats above-mentioned experiment with embodiment 2 to 6 is extract obtained, and effect is with above-mentioned, and it is tied
Fruit all shows that extract has good safety compared with azone.
Four, embodiment 1, embodiment 2, the experiment of embodiment 3 gained Chinese medicine extract A, B, C transdermal effect.
1. experiment purpose
1. experimental verification embodiment 1, embodiment 2,3 three kinds of prescriptions of embodiment are for the penetration with skin.
2. experiment content
2.1 instruments and reagent
2.1.1 instrument: high performance liquid chromatograph；Franz Transdermal absorption instrument；Analytical balance；Ultrasound Instrument；Pipet；Capacity
Bottle；Turbula shaker, Corium Mus (Beijing gold shepherd laboratory animal cultivates company)；Brown bottle；Spectrophotometer
2.1.2 reagent normal saline (being purchased from Kelun Pharm Ind Co., Ltd., Sichuan, lot number M13072421)；Water-soluble nitrogen
Ketone, (being purchased from Beijing Brilliance Biochemical Co., Ltd.)；Anhydrous glucose；Phenol；NaNO2；10%Al (NO3)3；
2.2 prepared by Corium Mus:
2.2.1 de-for Cavia porcellus (Beijing Military Medical Academy animal center) cervical vertebra is put to death；
2.2.2 rapidly its belly wool medical scissors is cut short as far as possible；
2.2.3 with depilatory cream, its belly wool is purified；
2.2.4 peel off skin of abdomen and remove subcutaneous fat, blood vessel, rinses to only repeatedly with distilled water；
2.2.5 with normal saline flushing several times, cold preservation in-80 DEG C of refrigerators is put standby.
2.3 Franz diffusion cells carry out transdermal test in vitro and run absorption in fact
2.3.1 Corium Mus: before experiment starts, is placed in normal saline immersion 15min by Corium Mus.
2.3.2 acceptable solution: normal saline.
2.3.3 for feeding: A, B, C
3. experimental technique
3.1 Franz diffusion cell experiments
The Corium Mus agrafe prepared is fixed between two Room diffusion cells, in the acceptance pool in vertical proliferation pond, adds 5mL
Mass fraction is the NaCl solution (normal saline) of 0.9%, sets acceptance pool mixing speed as 400 revs/min, sets
In temperature chamber, water temperature is 37 scholar 0.1 DEG C.It is separately added in supply pool for feeding, seals with preservative film suitable for reading.Work as sample
Product infiltration l, 2,4,6,8,10,12,24 are little constantly, and separately sampled V2 puts in tool plug centrifuge tube, every sub-sampling
While in acceptance pool, supplement the acceptable solution of equivalent and get rid of the bubble in pond.According to the difference of measured object, use not
Sample concentration in same detection method detection acceptable solution.Percutaneous permeability Q (mg/cm is calculated according to formula (1)2)。
Note: Q is Percutaneous permeability, and S is transdermal diffusion area (our diffusion cell R=0.6cm, S=1.1304cm2), V1
For Franz diffusion cell acceptable solution volume (V1=5mL), ρnThe mass concentration (mg/mL) of acceptable solution when sampling for n-th,
ρiThe mass concentration (mg/mL) of acceptable solution, V when sampling for i & lt2For sampling amount.
After being calculated by formula (1), make the curve chart of cumulant and time.
3.2 Phenol sulfuric acid procedure detection saccharides promote thoroughly
3.2.1 preparation of reagents
The preparation of standard solution: accurately weigh anhydrous glucose 100.0mg, is placed in 100ml volumetric flask and uses distilled water constant volume,
Obtaining concentration is 1mg/ml glucose stock solution.
The preparation of phenol solution: phenol 10.00g, addition water 190ml obtains 5% phenol solution and is placed in brown bottle.
3.2.2 experimental procedure
The drafting of standard curve:
Accurately draw 1mg/ml Glucose standards solution 1.0,2.0,3.0,4.0,5.0ml and be placed in 500ml volumetric flask
Constant volume.Accurately draw each 2ml of this serial solution, measure 2.0ml deionized water and do blank, be respectively placed in and have
In scale test tube, carry out chrominance response, draw standard curve.
Sample determination:
A. take and treat that test sample 2.0ml is put in test tube (doing blank with 2.0ml deionized water), add 1.0ml 5% phenol
Solution mixes.
B. 5.0ml concentrated sulphuric acid is added, after mixing 5min, tube sealing boiling water bath 1 hour.
C. it is cooled to room temperature after taking out, at 490nm, surveys absorbance.
3.2.3 standard curve:
Y=[(X+0.0049)/0.0101] × extension rate, R2=0.9999 wherein, and X is absorbance, and Y is polysaccharide
Content (ug/ml), X scope should fall between 0.2-0.8.
3.3 Forint phenol methods detect many peptides and promote thoroughly
3.3.1 preparation of reagents
Reagent first:
(A)10g Na2CO3+ 2g NaOH+0.25g sodium potassium tartrate tetrahydrate (KNaC4H4O4·4H2O) be dissolved in 500ml go from
Sub-water.
(B) 0.5g copper sulfate (CuSO4·5H2O) 100ml deionized water it is dissolved in.Use 50 parts (A) and 1 every time
Part (B) mixing, is reagent first.
Reagent second:
Forint phenol: deionized water=1:1 mixing.
3.3.2 experimental procedure
(1) 1ml sample solution (by surveying OD value requirement dilute sample) is taken.
(2) add 5ml reagent first, mix rapidly at vortex mixer and put into 25 DEG C of water-baths 10 minutes.
(3) add 0.5ml reagent second by pipe, mix immediately, 25 DEG C of water-baths 30 minutes.
(4) at 700nm, absorbance is measured.
3.3.3 standard curve
Y=[(X-0.0415)/0.0027] × extension rate, R2=0.9999 wherein, and X is absorbance, and Y is
Content of peptides (ug/ml), X scope should fall between 0.2-0.8.
3.4 sodium nitrite-aluminum sulfate method detection flavonoid promotees thoroughly
3.4.1 experimental principle
Flavone compound can react the flavone aluminium complex generating yellow with aluminum nitrate.
3.4.2 experimental procedure
(1) 1ml sample solution is taken, matched group 1ml deionized water.
(2) NaNO of 0.3ml 5% is added2Solution, shakes up.
(3) Al (NO of 0.3ml 10% is added3)3, shake up, stand 3 minutes.
(4) add 4.0ml deionized water, shake up.
(5) add the NaOH of 4.0ml 4%, shake up, stand 10 minutes, at 510nm, measure absorbance.
3.4.3 standard curve
Y=(1.101X+0.0029) × extension rate, R2=0.9999 wherein X be absorbance, Y is flavones content (ug/ml),
X scope should fall between 0.2～0.8.
4. result and analysis
4.1 Chinese medicine extract of the present invention promote result to the active polysaccharides from plants (trehalose) in cosmetics
A kind of natural saccharide of trehalose, is present in many desert plants, and when plant dries up, formation one layer is glassy
Substrate, can effectively protect epidermis cell membrane structure, activating cell, nurse one's health skin, be representative in the middle of cosmetics
Active polysaccharides from plants.
Running absorption experiment by Franz diffusion cell transdermal, the different Chinese prescriptions of detection 1% are saturating to the rush of 1% trehalose
Effect, detects transdermal concentration with phend-sulphuric acid, using 1% azone as positive control.Testing result such as Fig. 5.From knot
It can be seen that after selected 3 kinds of extracts use 1h, all produce and promote saturating effect in Guo；Percutaneous permeability reaches 6mg/cm2
Time each penetration enhancer used by time embodiment 1 ＜ embodiment 3 ＜ embodiment 2 ＜ 1% azone, wherein embodiment 1 required time
It is only the 40% of 1% azone, promotees onset rapid；Selected 24 hours trehalose accumulation infiltration capacities of 3 kinds of Chinese medicine penetration enhancer are divided
It is not: embodiment 1 is 10.448mg/cm2, embodiment 2 is 8.368mg/cm2, embodiment 3 is 8.459mg/cm2,
1.Wherein, the embodiment 1 saturating best results of rush to trehalose, the accumulation milliosmolarity of its 24h is about blank (5.047
mg/cm2) twice, and higher than positive control azone (7.089mg/cm2).The different penetration enhancer rush to trehalose
Effect is thoroughly: embodiment 1 > embodiment 3 > embodiment 2 > 1% azone.
Result above shows that extract of the present invention has compared with prior art and promotees rapidly saturating effect, in skin lash-up recovering
Quick-acting rush are the most crucial, can play the effect quickly promoting thoroughly to repair within the prime time of skin repair.Inventor
Utilizing embodiment 4,5,6 to repeat above-mentioned experiment, experimental result is with above-mentioned.
4.2 different materials promote saturating result to many peptides
Running absorption experiment by Franz diffusion cell transdermal, the Chinese medicine penetration enhancer of detection 1% is saturating to the rush of 10% oat polypeptide
Effect, detects transdermal concentration with Forint phenol method, using 1% azone as positive control.Testing result such as Fig. 6.From result
It can be seen that after selected 3 kinds of Chinese medicine extract use 3h, all produce and promote saturating effect.Oat polypeptide accumulation infiltration in 24 hours
It is 0.35mg/cm that amount is respectively as follows: example 12, example 2 is 0.33mg/cm2, example 3 is 0.25mg/cm2.Wherein, real
Executing the example 1 saturating best results of rush to oat polypeptide, the accumulation milliosmolarity of its 24h is about blank (0.13mg/cm2)
Nearly 3 times, and than positive control azone (0.21mg/cm2) improve 66%, illustrate that embodiment 1 is long-acting to oat polypeptide
Promote effect and be better than azone.Saturating effect of rush of oat polypeptide is by different penetration enhancer: example 1 > example 2 > example 3 > 1% nitrogen
Ketone.
Result above shows that extract of the present invention has, compared with prior art, effect that long-acting rush is saturating, the most long-acting
Promote thoroughly to make the infiltration skin that nutritional labeling is the most lasting, make skin effect optimal, inventor utilize embodiment 4,5,
6 repeat above-mentioned experiment, and experimental result is with above-mentioned.
4.3 Chinese medicine extract promote result to vegetable active flavone (licoflavone)
Licoflavone is the natural whiting agent extracted from Radix Glycyrrhizae, and it can suppress the activity of tryrosinase, can suppress again many
Bar pigment change and the oxidasic activity of DHICA, be a kind of representational vegetable active flavone cosmetics additive.
Absorption experiment, the Chinese medicine penetration enhancer of detection 1% rush to 5% licoflavone is run by Franz diffusion cell transdermal
Effect, detects transdermal concentration with Sodium nitrite-aluminium nitrite method, using 1% azone as positive control thoroughly.Testing result is such as
Fig. 7.
As can be seen from the results, 24 hours licoflavone accumulation infiltration capacities of selected 3 kinds of Chinese medicine penetration enhancer are respectively as follows: reality
Execute example 1 for 0.23mg/cm2, embodiment 2 is 0.2mg/cm2, embodiment 3 is 0.13mg/cm2, azone is
0.12mg/cm2,Blank assay is 0.08mg/cm2.Wherein, the embodiment 1 saturating best results of rush to licoflavone, its
The accumulation infiltration capacity of 24h is blank (0.08mg/cm2) nearly 3 times, and be approximately positive control azone
(0.12mg/cm2) 2 times, illustrate that the embodiment 1 saturating effect of rush long-acting to licoflavone is better than azone.Different penetration enhancer pair
Saturating effect of rush of licoflavone is: embodiment 1 > embodiment 2 > embodiment 3 > 1% azone > blank assay.
Result above shows that extract of the present invention has, compared with prior art, effect that long-acting rush is saturating, and inventor utilizes embodiment
4,5,6 repeat above-mentioned experiment, and experimental result is with above-mentioned.
Claims (10)
1. one kind has the external medicine composition promoting that skin active material runs absorption effect, it is characterised in that institute
State external medicine composition to be made up of the crude drug of following weight parts proportioning:
Rhizoma Ligustici 2～6, Radix Angelicae Sinensis 1～4, Radix Salviae Miltiorrhizae 1～4, Cortex Cinnamomi 1～3, Ampelopsis grossedentata 1～3.
2. one kind has the external medicine composition promoting that skin active material runs absorption effect, it is characterised in that institute
State external medicine composition to be made up of the crude drug of following weight parts proportioning:
Rhizoma Ligustici 2～6, Radix Angelicae Sinensis 1～4, Radix Salviae Miltiorrhizae 1～4, Cortex Cinnamomi 1～3, Ampelopsis grossedentata 1～3, Herba Menthae 1～3, Fructus Corni 1～
3。
3. the preparation method of external medicine composition described in a claim 1 or 2, it is characterised in that described system
The step of Preparation Method is as follows:
(1) crude drug described in claim 1 or 2 is weighed by described weight after pulverizing, mixing；
(2) percent by volume is 60%～90% ethanol extraction, crude drug and ethanol mass volume ratio g/ml be 1:10～
1:30,70 DEG C～90 DEG C are extracted 1～4 hour；
(3) extracting solution that step (2) obtains is cooled to 20 DEG C～30 DEG C, 200～400 mesh coarse filtrations, leach
Carry out vacuum filtration after medicinal residues, collect secondary filtrate；
(4) whole for filtrate rotary evaporation ethanol are obtained concentrated solution, concentrated solution adds 1,3 butylene glycol, addition
For step (3) gained secondary amount of filtrate percent by volume 60%～90%；Miscible uniformly, filter, take clear liquid, i.e.
?.
4. preparation method as claimed in claim 3, it is characterised in that the volume hundred of ethanol in described step (2)
Proportion by subtraction is 70%, and crude drug and ethanol mass volume ratio g/ml are 1:15；Extraction time is 3 hours.
5. preparation method as claimed in claim 3, it is characterised in that vacuum filtration condition in described step (3)
Vacuum filtration is carried out for spreading folder 0.3～0.6cm kieselguhr in the middle of two layers of filter paper, filter paper in buchner funnel.
6. preparation method as claimed in claim 3, it is characterised in that described step (4) is in vacuum
-0.09MPa, the whole ethanol of rotary evaporation under the conditions of 40 DEG C～50 DEG C.
7. one kind has the external application Chinese medicine extract promoting that skin active material runs absorption effect, it is characterised in that
Described external application Chinese medicine extract is obtained by preparation method extraction described in any one in claim 3 to 6.
8. external application Chinese medicine extract described in claim 7 has promotion skin active material operation absorption effect in preparation
External use skin care additive in purposes.
9. one kind has the skin care product additive promoting that skin active material runs absorption effect, it is characterised in that institute
State skin care product additive sterilized rear prepared by external application Chinese medicine extract described in claim 7.
10. skin care product additive as claimed in claim 9, it is characterised in that described sterilizing program is 90 DEG C of water-baths
Sterilizing 20～40 minutes.
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| CN112120970A (en) * | 2020-09-29 | 2020-12-25 | 上海相宜本草化妆品股份有限公司 | Essential oil composition, preparation method and application thereof |
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| CN102018797A (en) * | 2010-11-26 | 2011-04-20 | 新时代健康产业（集团）有限公司 | Traditional Chinese medicine composition with allergy soothing effect, preparation and preparation method thereof |
| CN102068398A (en) * | 2010-11-26 | 2011-05-25 | 新时代健康产业（集团）有限公司 | Allergy-relieving, anti-inflammatory and anti-irritation skin-care composition, preparation and preparation method thereof |
| CN102247308A (en) * | 2011-06-14 | 2011-11-23 | 北京华夏众芳生物科技有限公司 | Infiltration-promoting Chinese medicinal composition, preparation and preparation method thereof |
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|---|---|---|---|---|
| CN102018797A (en) * | 2010-11-26 | 2011-04-20 | 新时代健康产业（集团）有限公司 | Traditional Chinese medicine composition with allergy soothing effect, preparation and preparation method thereof |
| CN102068398A (en) * | 2010-11-26 | 2011-05-25 | 新时代健康产业（集团）有限公司 | Allergy-relieving, anti-inflammatory and anti-irritation skin-care composition, preparation and preparation method thereof |
| CN102247308A (en) * | 2011-06-14 | 2011-11-23 | 北京华夏众芳生物科技有限公司 | Infiltration-promoting Chinese medicinal composition, preparation and preparation method thereof |
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| CN112120970A (en) * | 2020-09-29 | 2020-12-25 | 上海相宜本草化妆品股份有限公司 | Essential oil composition, preparation method and application thereof |
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Application publication date: 20161005 |