CN104922649B - Turmeric acne eliminating external preparation and preparation method and application thereof - Google Patents
Turmeric acne eliminating external preparation and preparation method and application thereof Download PDFInfo
- Publication number
- CN104922649B CN104922649B CN201510426083.5A CN201510426083A CN104922649B CN 104922649 B CN104922649 B CN 104922649B CN 201510426083 A CN201510426083 A CN 201510426083A CN 104922649 B CN104922649 B CN 104922649B
- Authority
- CN
- China
- Prior art keywords
- ethanol
- herba
- rhizoma
- solution
- amount
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention relates to a turmeric acne eliminating external preparation comprising turmeric, coral ginger and other components, a preparation method of the turmeric acne eliminating external preparation and the novel application in the field of pharmacy, and belongs to the technical field of traditional Chinese medicine. The turmeric acne eliminating external preparation has the effect of relieving acute injuries and swelling of soft tissue and improving pathological tissue change and can be used for treating traumatic injuries.
Description
Technical field
The present invention relates to a kind of eliminating acne turmeric external preparation containing compositions such as Rhizoma Curcumae Longae, Rhizoma Zingiberis corallinis and preparation method thereof and system
New application in medicine field, belongs to the technical field of Chinese medicine.
Background technology
Eliminating acne turmeric liniment is OTC (over-the-counter) class liniment, and main component is Rhizoma Curcumae Longae, Rhizoma Paridis, Herba Polygoni cymosi, Herba Solidaginiss, native chaste tree
Mustard, Herb Gynostemmae Pentaphylli, Rhizoma Zingiberis corallini, exercise use is for the acne (acne) caused by damp and hot strongly fragrant skin.The preparation method of existing product is such as
Under：Take Rhizoma Curcumae Longae, Rhizoma Zingiberis corallini and be ground into coarse powder, vapor distillation extracts volatile oil, standby.Herb Gynostemmae Pentaphylli, Herba Solidaginiss, Rhizoma Paridis are crushed
Into fine powder, according to the percolation under fluid extract and extractum item（Chinese Pharmacopoeia one annex I O of version in 2000）With 6 times of amounts 80%
Ethanol as solvent carries out percolation, collects liquid of filtering, standby.Herba Polygoni cymosi, Herba Chenopodii are taken, is added water to cook three times, 2 hours for the first time, the
Secondary 1.5 hours, third time 1 hour, collecting decoction, filtration, filtrate was concentrated into relative density for 1.18～1.22（60℃）'s
Clear paste, plus ethanol is to alcohol content up to 50%, stands filtration in 24 hours, filtrate merges with above-mentioned liquid of filtering.Separately take volatile oil addition polymerization Pyrusussuriensiss
Add after the emulsifying of ester 80 in above-mentioned medicinal liquid, mix, filtration is adjusted to ormal weight, is obtained final product.
The content of the invention
It is an object of the invention to provide the new preparation method of the Chinese patent medicine and the new opplication in pharmacy.
In fact, the present invention relates to composition is Rhizoma Curcumae Longae, Rhizoma Paridis, Herba Polygoni cymosi, Herba Solidaginiss, Herba Chenopodii, Herb Gynostemmae Pentaphylli, Rhizoma Zingiberis corallini
Eliminating acne turmeric external preparation and preparation method thereof and the application in the medicine for preparing treatment traumatic injury.
The technical scheme is that such composition：Described eliminating acne turmeric external preparation is by 50 parts of Rhizoma Curcumae Longae, Rhizoma Paridis 50
Part, 50 parts of Herba Polygoni cymosi, 25 parts of Herba Chenopodii, 25 parts of Herba Solidaginiss, 25 parts of Herb Gynostemmae Pentaphylli, 50 parts of compositions of Rhizoma Zingiberis corallini.Described Rhizoma Curcumae Longae disappears
The preparation method of Cuo external preparation is as follows：Take Rhizoma Curcumae Longae, Rhizoma Zingiberis corallini and be ground into coarse powder, using supercritical CO2Fluids extraction is extracted, and must be carried
Thing A is taken, is retained；Herb Gynostemmae Pentaphylli, Herba Solidaginiss, Rhizoma Paridis are ground into fine powder, merge medicinal residues and Herba Polygoni cymosi, Herba Chenopodii after said extracted,
With 6 times of amount 80% alcohol reflux 2 times, extracting solution is filtered, and 60 DEG C to relative density of filtrate recycling ethanol is 1.05～1.10,
2 times of amount water are added to filter after stirring evenly, filtrate crosses AB-8 macroporous adsorptive resins with the flow velocity of per kilogram medical material 250ml per hour,
Again with 10% ethanol remove impurity of 4 times of resin volumes, finally with 50% ethanol desorbing of 4 times of resin volumes, stripping liquid is collected, reduced pressure back
Ethanol is received, concentrate drying is crushed, the soybean lecithin of 4 times of amounts of powder of getting it filled：Cholesterol=8:1 mixture, plus ethanol in proper amount is molten
Solution, is spin-dried for, and add water the solution for making 10%, then homogenizing 30 minutes, obtains solution B, and liniment, gel, Emulsion, spraying is then obtained
Agent, ointment.Supercritical CO2Fluid extraction condition is as follows：Extracting pressure is：30MPa；Extraction temperature is：45℃；CO2Flow：
40L/h；Extraction time：3 hours；Entrainer is ethanol, and consumption is 1ml/g.The eliminating acne turmeric external preparation is preparing treatment
Application in the medicine of traumatic injury.
Experimental example 1：Acute soft tissue contusion is tested
150-200 g rats, male, 20, random packet, 10 per group.Rats with bilateral hind leg calf shaving, positioning
The normal girth of measurement each group rat hindleg shank, continuous strike 3 times on the outside of mid calf with pounder, cause local during modeling
Acute soft tissue injury model, per group of 20 lower limbs, totally 80 lower limbs.If merging fracture person to be rejected.Instant coating after modeling, often
Secondary 0.5 mL, four times a day.8h after 1st coating, 24h, 48h, 72 h determine hind leg shank swelling (girth after modeling coating-
Normal girth), while per group is respectively quickly put to death rat cervical dislocation in this 4 time points, at the centre of percussion position group is taken
Knit, 10% formaldehyde is fixed, paraffin embedding, HE dyeing.Optical microphotograph Microscopic observation inflammation swelling and inflammatory cell infiltration situation.
Pharmacodynamic experiment result shows that this pharmaceutical composition has the effect of mitigation acute soft tissue contusion swelling, and changes
The pathological change of kind tissue, shows as subcutaneous tissue and muscle tissue damage mitigates, neutrophil cell infiltration is reduced, tissue is repaiied
It is multiple very fast.Can be used to treat traumatic injury.
But composition is sufficiently complex contained by Chinese medicine, both containing various active ingredients, there is invalid element again, existing process dosage form has
Certain defect, should extract its effective ingredient and further separated, purification, then carry out it is liposomal encapsulated, in order to transdermal
Absorb.Simultaneously preparation is made into the external preparation such as liniment, gel, Emulsion, spray, ointment.Through testing repeatedly, this product
The technique of product can to greatest extent extract the active ingredient in this prescription medical material so that the inherent quality and clinic of this product preparation is controlled
Therapeutic effect is improved, and the effect for enabling product is played to greatest extent.
Experimental example 2：Volatile oil extracting mode is investigated
Mode is 1.：Take Rhizoma Curcumae Longae, Rhizoma Zingiberis corallini and be ground into coarse powder, add 6 times of amount water to soak 2 hours, then steam distillation 5 is little
When, collect volatile oil.
Mode is 2.：Take Rhizoma Curcumae Longae, Rhizoma Zingiberis corallini and be ground into coarse powder, using supercritical CO2Fluids extraction is extracted.Extracting pressure
For：30MPa；Extraction temperature is：45℃；CO2Flow：40L/h；Extraction time：3 hours；Entrainer is ethanol, and consumption is 1ml/
g。
From the experiment above result can be seen that can not only increase volatile ingredient using the method for supercritical extraction
Extracted amount, and can be so that part curcumin is extracted in the case of low temperature, to active component by adding entrainer
It is a protection well, therefore the extracting mode of our selected volatile ingredients is supercritical extraction.
Experimental example 3：Active site enrichment route screening
Route is 1.：Take Rhizoma Curcumae Longae, Rhizoma Zingiberis corallini and be ground into coarse powder, using supercritical CO2Fluids extraction is extracted, and extract is protected
Stay.Herb Gynostemmae Pentaphylli, Herba Solidaginiss, Rhizoma Paridis, fine powder is ground into, carries out percolation with 6 times of 80% ethanol as solvent of amount, collection is filtered liquid, standby
With.Herba Polygoni cymosi, Herba Chenopodii are taken, is added water to cook three times, 2 hours for the first time, second 1.5 hours, third time 1 hour merged and decocts
Liquid, filtration.Filtrate is concentrated into relative density for 1.18～1.22（60℃）Clear paste, plus ethanol is to amount of alcohol up to 50%, stands 24
Hour filtration, filtrate merges with above-mentioned liquid of filtering, decompression recycling ethanol, concentrate drying.
Route is 2.：Take Rhizoma Curcumae Longae, Rhizoma Zingiberis corallini and be ground into coarse powder, using supercritical CO2Fluids extraction is extracted, and extract is protected
Stay.Herb Gynostemmae Pentaphylli, Herba Solidaginiss, Rhizoma Paridis are ground into fine powder, merge medicinal residues and Herba Polygoni cymosi, Herba Chenopodii after said extracted, with 6 times of amounts
80% alcohol reflux 2 times, extracting solution is filtered, and filtrate recycling ethanol to relative density is 1.05～1.10（60℃）, add 2
Times amount water is filtered after stirring evenly, and filtrate crosses AB-8 macroporous adsorptive resins with the flow velocity of per kilogram medical material 250ml per hour, then with 4
10% ethanol remove impurity of times resin volume, finally with 50% ethanol desorbing of 4 times of resin volumes, collects stripping liquid, recovered under reduced pressure second
Alcohol, concentrate drying is crushed.
From the experiment above result can be seen that process route 2. can effective deposition activity position so that content is big
Width is lifted, and the rate of transform is higher.
Experimental example 4：Liposome includes conditional filtering
（1）Soybean lecithin is studied with cholesterol consumption proportion
Method：Weigh a certain amount of soybean lecithin to be placed in 500ml eggplant-shape bottles with cholesterol, add appropriate dehydrated alcohol
Dissolving is made, on Rotary Evaporators, is evaporated under reduced pressure in 35 DEG C of waters bath with thermostatic control（Rotating speed is 80r/min）, ethanol is reclaimed, use 10%
Ointment amidin aquation, rotate 10 minutes, then homogenizing 30 minutes.
Rhizoma Paridis saponin envelop rate
It can be seen from the results above that with the increase of soybean lecithin consumption, envelop rate is presented ascendant trend.But with reference to rising
Amplitude and consumption increase ratio, we select soybean lecithin：Cholesterol is 8:1 ratio.
（2）Ointment powder concentration affects on envelop rate
Method：Weigh a certain amount of soybean lecithin and cholesterol admixture（Both ratios are 8:1）, it is placed in 500ml eggplants
In shape bottle, add appropriate dehydrated alcohol to make dissolving, on Rotary Evaporators, be evaporated under reduced pressure in 35 DEG C of waters bath with thermostatic control（Rotating speed is
80r/min）, ethanol is reclaimed, the ointment amidin aquation of variable concentrations is added, rotate 10 minutes, then homogenizing 30 minutes.
Rhizoma Paridis saponin envelop rate
It can be seen from the results above that medicated powder excessive concentration, causes to disperse insufficient, envelop rate is decreased obviously；But from test
It is too low that process can be seen that concentration so that volumes of formulation is excessively huge, for preparations shaping has considerable influence, therefore selects medicated powder
Concentration of aqueous solution is 10%.
Experimental example 5：Acute soft tissue contusion contrast experiment
150-200 g rats, male, 20, random packet, 10 per group.Rats with bilateral hind leg calf shaving, positioning
The normal girth of measurement each group rat hindleg shank, continuous strike 3 times on the outside of mid calf with pounder, cause local during modeling
Acute soft tissue injury model, per group of 20 lower limbs, totally 80 lower limbs.If merging fracture person to be rejected.Instant coating after modeling, often
Secondary 0.5 mL, four times a day.8h after 1st coating, 24h, 48h, 72 h determine hind leg shank swelling (girth after modeling coating-
Normal girth), while per group is respectively quickly put to death rat cervical dislocation in this 4 time points, at the centre of percussion position group is taken
Knit, 10% formaldehyde is fixed, paraffin embedding, HE dyeing.Optical microphotograph Microscopic observation inflammation swelling and inflammatory cell infiltration situation.
Rhizoma Paridis saponin preparation technology：Rhizoma Paridis medical material, crushes, and with 6 times of amount 80% alcohol reflux 2 times, extracting solution is filtered,
Filtrate recycling ethanol to relative density is 1.05～1.10（60℃）, add 2 times of amount water to filter after stirring evenly, filtrate is with per kilogram medicine
The flow velocity of material 250ml per hour crosses AB-8 macroporous adsorptive resins, then with 10% ethanol remove impurity of 4 times of resin volumes, finally with 4
50% ethanol desorbing of times resin volume, collects stripping liquid, and decompression recycling ethanol, concentrate drying is crushed.
It can be seen from the results above that this pharmaceutical composition deposition activity position, compared with one-component（Rhizoma Paridis saponin）Effect
More preferably.The technique of offer is further separated to active ingredient, purification, then carry out it is liposomal encapsulated, in order to transdermal suction
Receive.The external hydrogel adhesive that we provide, can extend administration time, play a part of slow release.
Summary experimental result, it can be deduced that it is an advantage of the current invention that：
（1）The preparation technology enrichment active ingredient of the present invention, is more easy to Transdermal absorption, and the effect of product is obviously improved.
（2）The present invention has excavated new medical application to known Chinese patent medicine eliminating acne turmeric liniment, opened up one it is new should
Use field.
Specific embodiment
Embodiment 1：Rhizoma Curcumae Longae 50g, Rhizoma Paridis 50g, Herba Polygoni cymosi 50g, Herba Chenopodii 25g, Herba Solidaginiss 25g, Herb Gynostemmae Pentaphylli 25g, coral
Coral Rhizoma Zingiberis Recens 50g, takes Rhizoma Curcumae Longae, Rhizoma Zingiberis corallini and is ground into coarse powder, using supercritical CO2Fluids extraction is extracted（Supercritical CO2Fluid extraction
Condition is as follows：Extracting pressure is：30MPa；Extraction temperature is：45℃；CO2Flow：40L/h；Extraction time：3 hours；Entrainer
For ethanol, consumption is 1ml/g）, extract A is obtained, retain；Herb Gynostemmae Pentaphylli, Herba Solidaginiss, Rhizoma Paridis are ground into fine powder, merge above-mentioned carrying
Rear medicinal residues and Herba Polygoni cymosi, Herba Chenopodii are taken, with 6 times of amount 80% alcohol reflux 2 times, extracting solution is filtered, and filtrate recycling ethanol is to phase
It is 1.05～1.10 to 60 DEG C of density, adds 2 times of amount water to filter after stirring evenly, filtrate is with the stream of per kilogram medical material 250ml per hour
Speed crosses AB-8 macroporous adsorptive resins, then with 10% ethanol remove impurity of 4 times of resin volumes, finally with 50% second of 4 times of resin volumes
Alcohol desorbing, collects stripping liquid, and decompression recycling ethanol, concentrate drying is crushed.The soybean lecithin of 4 times of amounts of powder of getting it filled：Cholesterol=8:
1 mixture, plus ethanol in proper amount dissolving, are spin-dried for, and add water the solution for making 10%, homogenizing 30 minutes, obtains solution B, adds 1%
940 type carbomers, stir 1 hour, then after extract A is dissolved with 2 times of amount Propylene Glycol, are added to 30 points of homogenizing in mixed solution
Clock, with triethanolamine pH=6～7, then homogenizing 30 minutes are adjusted, and obtain final product gel.
Usage and dosage：External.Ointment is applied to into affected part, outer wiping is daily 2-3 time.
Embodiment 2：Rhizoma Curcumae Longae 50g, Rhizoma Paridis 50g, Herba Polygoni cymosi 50g, Herba Chenopodii 25g, Herba Solidaginiss 25g, Herb Gynostemmae Pentaphylli 25g, coral
Coral Rhizoma Zingiberis Recens 50g, takes Rhizoma Curcumae Longae, Rhizoma Zingiberis corallini and is ground into coarse powder, using supercritical CO2Fluids extraction is extracted（Supercritical CO2Fluid extraction
Condition is as follows：Extracting pressure is：30MPa；Extraction temperature is：45℃；CO2Flow：40L/h；Extraction time：3 hours；Entrainer
For ethanol, consumption is 1ml/g）, extract A is obtained, retain；Herb Gynostemmae Pentaphylli, Herba Solidaginiss, Rhizoma Paridis are ground into fine powder, merge above-mentioned carrying
Rear medicinal residues and Herba Polygoni cymosi, Herba Chenopodii are taken, with 6 times of amount 80% alcohol reflux 2 times, extracting solution is filtered, and filtrate recycling ethanol is to phase
It is 1.05～1.10 to 60 DEG C of density, adds 2 times of amount water to filter after stirring evenly, filtrate is with the stream of per kilogram medical material 250ml per hour
Speed crosses AB-8 macroporous adsorptive resins, then with 10% ethanol remove impurity of 4 times of resin volumes, finally with 50% second of 4 times of resin volumes
Alcohol desorbing, collects stripping liquid, and decompression recycling ethanol, concentrate drying is crushed；The soybean lecithin of 4 times of amounts of powder of getting it filled：Cholesterol=8:
1 mixture, plus ethanol in proper amount dissolving, are spin-dried for, and add water the solution for making 10%, homogenizing 30 minutes, obtains solution B.Separately take extract
A adds Tween-80 and glycerol mix homogeneously, in 70 DEG C of homogenizing 10 minutes, is cooled to room temperature, adds Propylene Glycol homogenized milk
Change 10 minutes, emulsifier proportion is extract A：Tween-80：Glycerol：Propylene Glycol（1:4:1:1
）In being eventually adding above-mentioned solution B, 0.1% ethyl hydroxybenzoate is added, mixed, stand filtration, constant volume obtains final product Emulsion.
Embodiment 3：Rhizoma Curcumae Longae 50g, Rhizoma Paridis 50g, Herba Polygoni cymosi 50g, Herba Chenopodii 25g, Herba Solidaginiss 25g, Herb Gynostemmae Pentaphylli 25g, coral
Coral Rhizoma Zingiberis Recens 50g, takes Rhizoma Curcumae Longae, Rhizoma Zingiberis corallini and is ground into coarse powder, using supercritical CO2Fluids extraction is extracted（Supercritical CO2Fluid extraction
Condition is as follows：Extracting pressure is：30MPa；Extraction temperature is：45℃；CO2Flow：40L/h；Extraction time：3 hours；Entrainer
For ethanol, consumption is 1ml/g）, extract A is obtained, retain；Herb Gynostemmae Pentaphylli, Herba Solidaginiss, Rhizoma Paridis are ground into fine powder, merge above-mentioned carrying
Rear medicinal residues and Herba Polygoni cymosi, Herba Chenopodii are taken, with 6 times of amount 80% alcohol reflux 2 times, extracting solution is filtered, and filtrate recycling ethanol is to phase
It is 1.05～1.10 to 60 DEG C of density, adds 2 times of amount water to filter after stirring evenly, filtrate is with the stream of per kilogram medical material 250ml per hour
Speed crosses AB-8 macroporous adsorptive resins, then with 10% ethanol remove impurity of 4 times of resin volumes, finally with 50% second of 4 times of resin volumes
Alcohol desorbing, collects stripping liquid, and decompression recycling ethanol, concentrate drying is crushed；The soybean lecithin of 4 times of amounts of powder of getting it filled：Cholesterol=8:
1 mixture, plus ethanol in proper amount dissolving, are spin-dried for, and add water the solution for making 10%, homogenizing 30 minutes, obtains solution B；Extract A adds
Enter 2 times of ethanol dissolvings, then mix homogeneously with solution B, adjustment concentration of alcohol is 30%, is mixed, and is filtered, filtrate is dispensed into pressure appearance
In device, pour compressed nitrogen and obtain final product spray.
Embodiment 4：Rhizoma Curcumae Longae 50g, Rhizoma Paridis 50g, Herba Polygoni cymosi 50g, Herba Chenopodii 25g, Herba Solidaginiss 25g, Herb Gynostemmae Pentaphylli 25g, coral
Coral Rhizoma Zingiberis Recens 50g, takes Rhizoma Curcumae Longae, Rhizoma Zingiberis corallini and is ground into coarse powder, using supercritical CO2Fluids extraction is extracted（Supercritical CO2Fluid extraction
Condition is as follows：Extracting pressure is：30MPa；Extraction temperature is：45℃；CO2Flow：40L/h；Extraction time：3 hours；Entrainer
For ethanol, consumption is 1ml/g）, obtain extract A reservation；Herb Gynostemmae Pentaphylli, Herba Solidaginiss, Rhizoma Paridis are ground into fine powder, merge said extracted
Medicinal residues and Herba Polygoni cymosi, Herba Chenopodii afterwards, with 6 times of 80% alcohol reflux of amount 2 times, extracting solution is filtered, and filtrate recycling ethanol is to relative
60 DEG C of density is 1.05～1.10, adds 2 times of amount water to filter after stirring evenly, and filtrate is with the flow velocity of per kilogram medical material 250ml per hour
AB-8 macroporous adsorptive resins are crossed, then with 10% ethanol remove impurity of 4 times of resin volumes, finally with 50% ethanol of 4 times of resin volumes
Desorbing, collects stripping liquid, and decompression recycling ethanol, concentrate drying is crushed；The soybean lecithin of 4 times of amounts of powder of getting it filled：Cholesterol=8:1
Mixture, plus ethanol in proper amount dissolving, be spin-dried for, add water the solution for making 10%, homogenizing 30 minutes, obtains solution B；Extract A is added
2 times of Propylene Glycol dissolvings, then mix homogeneously with solution B, adjustment concentration of alcohol is 30%, is mixed, and is filtered, and filtrate is dispensed into pressure appearance
In device, pour compressed nitrogen and obtain final product spray.
Embodiment 5：Rhizoma Curcumae Longae 50g, Rhizoma Paridis 50g, Herba Polygoni cymosi 50g, Herba Chenopodii 25g, Herba Solidaginiss 25g, Herb Gynostemmae Pentaphylli 25g, coral
Coral Rhizoma Zingiberis Recens 50g, takes Rhizoma Curcumae Longae, Rhizoma Zingiberis corallini and is ground into coarse powder, using supercritical CO2Fluids extraction is extracted（Supercritical CO2Fluid extraction
Condition is as follows：Extracting pressure is：30MPa；Extraction temperature is：45℃；CO2Flow：40L/h；Extraction time：3 hours；Entrainer
For ethanol, consumption is 1ml/g）, obtain extract A reservation；Herb Gynostemmae Pentaphylli, Herba Solidaginiss, Rhizoma Paridis are ground into fine powder, merge said extracted
Medicinal residues and Herba Polygoni cymosi, Herba Chenopodii afterwards, with 6 times of 80% alcohol reflux of amount 2 times, extracting solution is filtered, and filtrate recycling ethanol is to relative
60 DEG C of density is 1.05～1.10, adds 2 times of amount water to filter after stirring evenly, and filtrate is with the flow velocity of per kilogram medical material 250ml per hour
AB-8 macroporous adsorptive resins are crossed, then with 10% ethanol remove impurity of 4 times of resin volumes, finally with 50% ethanol of 4 times of resin volumes
Desorbing, collects stripping liquid, and decompression recycling ethanol, concentrate drying is crushed；The soybean lecithin of 4 times of amounts of powder of getting it filled：Cholesterol=8:1
Mixture, plus ethanol in proper amount dissolving, be spin-dried for, add water the solution for making 10%, homogenizing 30 minutes, obtains solution B；By said extracted
Thing A adds 2 times of tween 80 emulsifyings, adds 2 times of amount glycerol mix homogeneously, adds solution B mix homogeneously, and adjustment ethanol is dense
Spend for 40%, homogenizing 20 minutes, filter, filtrate fill obtains final product liniment.
Experimental example 6：Rhizoma Curcumae Longae 50g, Rhizoma Paridis 50g, Herba Polygoni cymosi 50g, Herba Chenopodii 25g, Herba Solidaginiss 25g, Herb Gynostemmae Pentaphylli 25g, coral
Coral Rhizoma Zingiberis Recens 50g, takes Rhizoma Curcumae Longae, Rhizoma Zingiberis corallini and is ground into coarse powder, using supercritical CO2Fluids extraction is extracted（Supercritical CO2Fluid extraction
Condition is as follows：Extracting pressure is:25MPa；Extraction temperature is：50℃；CO2Flow：30L/h；Extraction time：4 hours；Entrainer
For ethanol, consumption is 0.8ml/g）, obtain extract A reservation；Herb Gynostemmae Pentaphylli, Herba Solidaginiss, Rhizoma Paridis are ground into fine powder, merge above-mentioned carrying
Rear medicinal residues and Herba Polygoni cymosi, Herba Chenopodii are taken, with 6 times of amount 80% alcohol reflux 2 times, extracting solution is filtered, and filtrate recycling ethanol is to phase
It is 1.05～1.10 to 60 DEG C of density, adds 2 times of amount water to filter after stirring evenly, filtrate is with the stream of per kilogram medical material 250ml per hour
Speed crosses AB-8 macroporous adsorptive resins, then with 10% ethanol remove impurity of 4 times of resin volumes, finally with 50% second of 4 times of resin volumes
Alcohol desorbing, collects stripping liquid, and decompression recycling ethanol, concentrate drying is crushed；The soybean lecithin of 3 times of amounts of powder of getting it filled：Cholesterol=9:
1 mixture, plus ethanol in proper amount dissolving, are spin-dried for, and add water the solution for making 6%, homogenizing 30 minutes, obtains solution B；Super face above-mentioned
Boundary's extract adds 2 times of tween 80 emulsifyings, adds 2 times of amount glycerol mix homogeneously, adds solution B mix homogeneously, adjusts second
Determining alcohol is 40%, homogenizing 20 minutes, is filtered, and filtrate fill obtains final product liniment.
Experimental example 7：Rhizoma Curcumae Longae 50g, Rhizoma Paridis 50g, Herba Polygoni cymosi 50g, Herba Chenopodii 25g, Herba Solidaginiss 25g, Herb Gynostemmae Pentaphylli 25g, coral
Coral Rhizoma Zingiberis Recens 50g, takes Rhizoma Curcumae Longae, Rhizoma Zingiberis corallini and is ground into coarse powder, using supercritical CO2Fluids extraction is extracted（Supercritical CO2Fluid extraction
Condition is as follows：Extracting pressure is：35MPa；Extraction temperature is：40℃；CO2Flow：50L/h；Extraction time：4 hours；Entrainer
For ethanol, consumption is 1.2ml/g）, obtain extract A reservation；Herb Gynostemmae Pentaphylli, Herba Solidaginiss, Rhizoma Paridis are ground into fine powder, merge above-mentioned carrying
Rear medicinal residues and Herba Polygoni cymosi, Herba Chenopodii are taken, with 6 times of amount 80% alcohol reflux 2 times, extracting solution is filtered, and filtrate recycling ethanol is to phase
It is 1.05～1.10 to 60 DEG C of density, adds 2 times of amount water to filter after stirring evenly, filtrate is with the stream of per kilogram medical material 250ml per hour
Speed crosses AB-8 macroporous adsorptive resins, then with 10% ethanol remove impurity of 4 times of resin volumes, finally with 50% second of 4 times of resin volumes
Alcohol desorbing, collects stripping liquid, and decompression recycling ethanol, concentrate drying is crushed；The soybean lecithin of 5 times of amounts of powder of getting it filled：Cholesterol=
10:1 mixture, plus ethanol in proper amount dissolving, are spin-dried for, and add water the solution for making 12%, homogenizing 30 minutes, obtains solution B；To extract
Thing A adds 2 times of tween 80 emulsifyings, adds 2 times of amount glycerol mix homogeneously, adds solution B mix homogeneously, and adjustment ethanol is dense
Spend for 40%, homogenizing 20 minutes, filter, filtrate fill obtains final product liniment.
Claims (3)
1. the preparation method of eliminating acne turmeric external preparation, it is characterised in that：Rhizoma Curcumae Longae 50g, Rhizoma Paridis 50g, Herba Polygoni cymosi 50g, Herba Chenopodii
25g, Herba Solidaginiss 25g, Herb Gynostemmae Pentaphylli 25g, Rhizoma Zingiberis corallini 50g, take Rhizoma Curcumae Longae, Rhizoma Zingiberis corallini and are ground into coarse powder, using supercritical CO2Fluid
Extraction is extracted, supercritical CO2Fluid extraction condition is as follows：Extracting pressure is：30MPa；Extraction temperature is：45℃；CO2Flow：
40L/h；Extraction time：3 hours；Entrainer is ethanol, and consumption is 1ml/g, obtains extract A, is retained；Herb Gynostemmae Pentaphylli, Herba Solidaginiss,
Rhizoma Paridis are ground into fine powder, merge medicinal residues and Herba Polygoni cymosi, Herba Chenopodii after said extracted, with 6 times of 80% alcohol reflux of amount 2 times,
Extracting solution is filtered, and filtrate recycling ethanol to 60 DEG C of relative density is 1.05-1.10, adds 2 times of amount water to filter after stirring evenly, filtrate with
The flow velocity of per kilogram medical material 250ml per hour crosses AB-8 macroporous adsorptive resins, then is removed with 10% ethanol of 4 times of resin volumes
It is miscellaneous, finally with 50% ethanol desorbing of 4 times of resin volumes, stripping liquid is collected, decompression recycling ethanol, concentrate drying is crushed；Get it filled
The soybean lecithin of 4 times of amounts of powder：Cholesterol=8:1 mixture, plus ethanol dissolving, are spin-dried for, and add water the solution for making 10%,
Matter 30 minutes, obtains solution B, adds 1% 940 type carbomers, stirs 1 hour, then extract A is dissolved with 2 times of amount Propylene Glycol
Afterwards, homogenizing 30 minutes in mixed solution are added to, with triethanolamine pH=6-7, then homogenizing 30 minutes is adjusted, obtain final product gel；
Or：Rhizoma Curcumae Longae 50g, Rhizoma Paridis 50g, Herba Polygoni cymosi 50g, Herba Chenopodii 25g, Herba Solidaginiss 25g, Herb Gynostemmae Pentaphylli 25g, Rhizoma Zingiberis corallini 50g,
Take Rhizoma Curcumae Longae, Rhizoma Zingiberis corallini and be ground into coarse powder, using supercritical CO2Fluids extraction is extracted, supercritical CO2Fluid extraction condition is as follows：
Extracting pressure is：30MPa；Extraction temperature is：45℃；CO2 flows：40L/h；Extraction time：3 hours；Entrainer is ethanol, is used
Measure as 1ml/g, obtain extract A reservation；Herb Gynostemmae Pentaphylli, Herba Solidaginiss, Rhizoma Paridis are ground into fine powder, merge medicinal residues and thick stick after said extracted
Plate is returned, Herba Chenopodii, and with 6 times of amount 80% alcohol reflux 2 times, extracting solution is filtered, filtrate recycling ethanol to 60 DEG C of relative density
For 1.05-1.10,2 times of amount water are added to filter after stirring evenly, filtrate crosses AB-8 macropores with the flow velocity of per kilogram medical material 250ml per hour
Adsorption resin column, then with 10% ethanol remove impurity of 4 times of resin volumes, finally with 50% ethanol desorbing of 4 times of resin volumes, collect
Stripping liquid, decompression recycling ethanol, concentrate drying is crushed；The soybean lecithin of 4 times of amounts of powder of getting it filled：Cholesterol=8:1 mixture,
Plus ethanol in proper amount dissolving, it is spin-dried for, add water the solution for making 10%, homogenizing 30 minutes, obtains solution B；Said extracted thing A is added into 2
Times tween 80 emulsifying, adds 2 times of amount glycerol mix homogeneously, adds solution B mix homogeneously, and adjustment concentration of alcohol is 40%,
Homogenizing 20 minutes, filters, and filtrate fill obtains final product liniment.
2. eliminating acne turmeric external preparation, it is characterised in that：It is made up of the preparation method described in claim 1.
3. application of the eliminating acne turmeric external preparation in the medicine for preparing treatment traumatic injury according to claim 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510426083.5A CN104922649B (en) | 2015-07-20 | 2015-07-20 | Turmeric acne eliminating external preparation and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510426083.5A CN104922649B (en) | 2015-07-20 | 2015-07-20 | Turmeric acne eliminating external preparation and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104922649A CN104922649A (en) | 2015-09-23 |
| CN104922649B true CN104922649B (en) | 2017-04-12 |
Family
ID=54110297
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510426083.5A Active CN104922649B (en) | 2015-07-20 | 2015-07-20 | Turmeric acne eliminating external preparation and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104922649B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108903541A (en) * | 2018-09-25 | 2018-11-30 | 四川新绿色药业科技发展有限公司 | A kind of medicine-filled pillow and preparation method thereof for wind-expelling pain-stopping |
-
2015
- 2015-07-20 CN CN201510426083.5A patent/CN104922649B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104922649A (en) | 2015-09-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102579576B (en) | Chuanxiongchatiao granule extracts and preparations thereof | |
| CN103316324B (en) | Traditional Chinese medicine foot-bathing liquid for treating rheumatoid disease | |
| CN102988264A (en) | Ginkgo leaf freckle removing whitening cream and preparation method thereof | |
| CN102302553A (en) | Extraction method of key components of Chinese magnoliavine fruit medicament | |
| CN105496917A (en) | Herba centellae anti-wrinkling face cream and preparation method thereof | |
| CN104740644A (en) | Preparation method of composition for promoting skin compliance and ingredient absorption of eye hydropathic compress agent | |
| JP6605036B2 (en) | Method for preparing a medicinal composition for preventing or treating liver and kidney damage-related diseases | |
| CN105194308A (en) | Health-preserving medicinal liquor and preparing method thereof | |
| CN103316192B (en) | Traditional Chinese medicine foot-bathing liquid for treating lumbocrural pain and preparation method thereof | |
| CN102579625A (en) | Preparation method of Chinese medicinal dropping pills for treating rheumatoid arthritis | |
| CN104922649B (en) | Turmeric acne eliminating external preparation and preparation method and application thereof | |
| CN102302615B (en) | Effective site group of daphne giraldii nitsche leaf, preparation method, medicinal composition and application thereof | |
| CN108403932A (en) | A kind of Chinese medicine compound prescription external preparation and its preparation method and application for treating steroid dependent dermatitis | |
| CN108514568A (en) | The preparation method and its medical usage of thiazides compounds in the achene of Siberian cocklebur | |
| CN108815060B (en) | A kind of fat-reducing ointment and preparation method thereof containing roselle | |
| CN104069140B (en) | A kind of method for preparing desmodium medicinal extract and its particle | |
| CN105920130A (en) | Preparation method and externally used ointment of traditional Chinese medicine for treating talalgia | |
| CN108524663A (en) | A kind of composition for alleviating pain and detumescence and preparation method thereof and preparation | |
| CN102309620B (en) | Chinese medicinal preparation for treating constipation and preparation method thereof | |
| CN106188083B (en) | A method of extracting 1, 8-Cineole volatile oil from folium artemisiae argyi | |
| CN105125436B (en) | A kind of composite whitening composition and preparation method | |
| CN102961535A (en) | Preparation method of pain-easing and collateral-activating tincture with high chrysophanol transfer rate | |
| CN108042682A (en) | A kind of Long Lu Zishuiqinggan ball and preparation method thereof | |
| CN103830426B (en) | One treats vasculitic Chinese medicine composition | |
| CN104667174B (en) | A kind of iquid capsule for treating coronary heart disease syndrome of yang deficiency and blood stasis and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 551400 Guizhou city of Guiyang Province high-tech industry planning area in Qingzhen City Applicant after: Guizhou Shuangsheng Pharmaceutical Co. Ltd. Address before: 551400 Guizhou city of Guiyang Province high-tech industry planning area in Qingzhen City Applicant before: Shumeida Pharmaceutical Factory Co., Ltd., Guiyang |
|
| COR | Change of bibliographic data | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 551400 high tech industry planning area, Qingzhen City, Guiyang City, Guizhou Province Patentee after: Mingyuan Pharmaceutical Co.,Ltd. Address before: 551400 high tech industry planning area, Qingzhen City, Guiyang City, Guizhou Province Patentee before: GUIZHOU SHUANGSHENG PHARMACEUTICAL Co.,Ltd. |