CN105962368A - Preparation method of dendrobium officinale fermentation liquid - Google Patents
Preparation method of dendrobium officinale fermentation liquid Download PDFInfo
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- CN105962368A CN105962368A CN201610309207.6A CN201610309207A CN105962368A CN 105962368 A CN105962368 A CN 105962368A CN 201610309207 A CN201610309207 A CN 201610309207A CN 105962368 A CN105962368 A CN 105962368A
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- 230000004151 fermentation Effects 0.000 title claims abstract description 65
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- 238000002360 preparation method Methods 0.000 title claims abstract description 38
- 241001076416 Dendrobium tosaense Species 0.000 title abstract 5
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 50
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention relates to a preparation method of dendrobium officinale fermentation liquid. The method comprises the following steps of (1) preparing food enzymatic hydrolysate; (2) preparing a dendrobium officinale extraction solution; (3) preparing a dendrobium officinale nutrition solution; (4) performing fermentation; (5) performing emulsification. The dendrobium officinale, moringa oleifera powder and various medical and edible materials are matched in a proper proportion, and are sufficiently mixed; then, lactobacilli are added for fermentation; single strains, i.e., the lactobacilli are used in the fermentation process; a purpose-made culture solution is used as the breeding ground; the fermentation efficiency is more sufficiently realized; the combination of sodium chloride and acetic acid is used as a buffer solution, so that the pH value of the solution is enabled to be maintained in the pre-control range in the fermentation process.
Description
Technical field
The invention belongs to health protection medicine field, relate to fermentation technique, the preparation method of a kind of Herba Dendrobii fermentation liquid.
Background technology
Growth of Dendrobium candidum is at the moon of overhanging cliff, and ancients' normal suspension cable precipice or archery gather.Herba Dendrobii throughout the year by the world it
Anima, inhales the elite of life, as health preserving superfine product, is deeply favored by imperial palace noble since ancient times.But due to its growth conditions ten
Dividing harshness, natural production is the most rare, more excessively excavates for a long time because of among the people, causes wild resource endangered.In international market
On, Herba Dendrobii is processed to a kind of Chinese medicine being " admirable in all its details ", and every kilogram of price about 3000 dollars, is
Expensive grass.Its effect is mainly reflected in: strengthening immunologic function, Herba Dendrobii has the function of nourishing YIN and benefiting blood.The Qing Dynasty's " property of medicine
Opinion " to say: essence, nourishing stomach-YIN, physical strength profiting are amassed in Herba Dendrobii energy the kidney invigorating.Containing abundant polysaccharose substance, there is enhancing immunity merit
The effect of energy.Modern pharmacological research also shows: Herba Dendrobii can improve stress ability, has good resisting fatigue, anoxia enduring is made
With；Defying age, Herba Dendrobii is classified as by Shennong's Herbal has the panacea that " make light of one's life by commiting suicide and prolong life " acts on.Modern pharmacology grinds
Studying carefully confirmation: Herba Dendrobii contains various trace elements, the good health and a long life for human body have close relationship, anti-ageing to human body
Old effect is than general medicine more extensively, more comprehensively.
Herba Dendrobii is the most only used to medicine or medicated diet, and applicant does not the most find to use with the form of fermentation liquid
Report and technical literature in daily life.
Summary of the invention
It is an object of the invention to overcome prior art not enough, it is provided that a kind of improve non-oxidizability and have and improve immunity function
The preparation method of Herba Dendrobii fermentation liquid.
The technical solution used in the present invention is:
The preparation method of a kind of Herba Dendrobii fermentation liquid, comprises the steps:
Preparation food enzymolysis solution: by etc. the purple rice of weight, Semen setariae, Semen Coicis, Poria, Fructus Jujubae, Fructus Mume, Pericarpium Citri Reticulatae, Fructus Crataegi (parched to brown)
Soak, by food: water is the weight ratio mixing of 1:8, the red bean powder of the 1.5% of the gross weight of addition food and water and material gross weight
1.5% 0.05%NaHCO3, after 80 DEG C of 15min that precook, add and adjust the total of pH value 5.5, addition food and water with citric acid
The 0.02% pectase 55 DEG C insulation enzymolysis 2h of weight, adds 1% hemicellulase of the gross weight of food and water, adds food
With 0.56% cellulase of the gross weight of water, add 0.5% papain of the gross weight of food and water, add food and water
0.04% phytase 1h of gross weight, mix Fructus Hordei Germinatus, Massa Medicata Fermentata 15h, filter, sterilization, obtain food enzymolysis solution, stand-by；
(2) preparation Herba Dendrobii extracting solution: by Herba Dendrobii water blancing 2min, cooling, by material: water is that the weight ratio of 1:10 adds
Water is pulled an oar, and then mixes with moringa powder, Radix Paeoniae Alba, Fructus Amomi, Rhizoma Polygonati Odorati, Semen Raphani, adds water to 2.5 times of the initial water yield,
Decoct, extract supernatant, obtain Herba Dendrobii extracting solution, stand-by；
(3) the preparation of Herba Dendrobii nutritional solution: by food enzymolysis solution with Herba Dendrobii extracting solution by weight the proportions for 4:1
It is mixed, mixed liquor is put in autoclave, be heated to 90 DEG C, and keep 5min, Herba Dendrobii nutritional solution can be obtained；
(4) fermentation: adding culture fluid in Herba Dendrobii nutritional solution, Herba Dendrobii nutritional solution and culture fluid weight ratio are 10:1, add
Enter lactic acid bacteria, sodium chloride and acetic acid mixed fermentation 1day, add the breast of above-mentioned lactic acid bacteria, sodium chloride and acetic acid each half weight
Acid bacterium, sodium chloride and acetic fermentation, obtain fermentation liquid；
(5) emulsifying: adding emulsifying agent in fermentation liquid, cross leaching clear liquid, after pasteurization, packaging gets product, emulsifying agent is food
With gelatin or carboxymethyl cellulose.
And, the content of the solid content of the food enzymolysis solution that step is (1) described is 8%～10%.
And, step (2) described in the solid content of Herba Dendrobii extracting solution be 4%～6%.
And, step (2) described in Herba Dendrobii, moringa powder, Radix Paeoniae Alba, Fructus Amomi, Rhizoma Polygonati Odorati, the parts by weight of Semen Raphani divide
It is not: 20～25 parts, 5～10 parts, 1～2 part, 1～2 part, 1～2 part, 1～2 part.
And, the (3) described lactic acid bacteria of step, sodium chloride, the parts by weight of total consumption of acetic acid are respectively 100～120 parts, 8～10
Part, acetic acid is 4～6 parts.
And, the preparation method of the culture fluid that step is (3) described is: maltose is soluble in water, puts into compound after standing 20min
Vitamin, the post-heating that stirs boils, and is cooled to after room temperature add Ovum Gallus domesticus album, stirs, stand-by, maltose, water, multiple
Close vitamin, the weight ratio of Ovum Gallus domesticus album is 100:10000:3:10.
Advantage of the present invention and good effect be:
The present invention is by the collocation by rational proportion of the various materials of Herba Dendrobii, moringa powder and integration of edible and medicinal herbs, first by food pair
Answer enzymolysis, be then sufficiently mixed with extracting solution, add lactic acid bacteria and ferment, and sweat uses single culture, i.e.
Lactic acid bacteria, using special culture fluid as hotbed, its fermentation efficiency plays more abundant, the combination conduct of sodium chloride and acetic acid
Buffer solution ensure that in sweat, solution ph is maintained in the range of pre-control.
In the present invention, the consumption of each component is through test of many times and the most constantly to contrast, it was found that involved in technical scheme
Amount ranges can produce unexpected effect, by effective fermentation, make Herba Dendrobii, moringa powder and Radix Paeoniae Alba main
Nutritive effect doubled, although the consumption of each component and effect thereof are only to embody with word and numerical value, but its operating procedure gram
Having taken many technology prejudice of the art, the Herba Dendrobii being just improved non-oxidizability and raising matrix immunity function is sent out
Ferment liquid.
Detailed description of the invention:
Below by specific embodiment, the invention will be further described, and following example are illustrative, is not determinate,
Protection scope of the present invention can not be limited with this.
Embodiment 1
The preparation method of a kind of Herba Dendrobii fermentation liquid, comprises the steps:
Preparation food enzymolysis solution: by etc. the purple rice of weight, Semen setariae, Semen Coicis, Poria, Fructus Jujubae, Fructus Mume, Pericarpium Citri Reticulatae, Fructus Crataegi (parched to brown)
Soak, by food: water is the weight ratio mixing of 1:8, the red bean powder of the 1.5% of the gross weight of addition food and water and material gross weight
1.5% 0.05%NaHCO3, it is allowed to easily to boil, removes raw meat, except bitter taste, after 80 DEG C of 15min that precook, add and adjust with citric acid
PH value 5.5, adds the 0.02% pectase 55 DEG C insulation enzymolysis 2h of the gross weight of food and water, adds food and the gross weight of water
1% hemicellulase, add 0.56% cellulase of the gross weight of food and water, add food and water gross weight 0.5%
0.04% phytase 1h of the gross weight of papain, addition food and water, mixes Fructus Hordei Germinatus, Massa Medicata Fermentata 15h, filters, sterilization,
Stand-by (solid content 8%～10%)；
(2) preparation Herba Dendrobii extracting solution: by Herba Dendrobii water blancing 2min, cooling, by material: water is that the weight ratio of 1:10 adds
Water is pulled an oar, and then mixes with moringa powder, Radix Paeoniae Alba, Fructus Amomi, Rhizoma Polygonati Odorati, Semen Raphani, adds water to 2.5 times of the initial water yield,
Decoct, extract supernatant, stand-by (solid content 4%～6%), Herba Dendrobii, moringa powder, Radix Paeoniae Alba, Fructus Amomi, Rhizoma Polygonati Odorati, Lay
The weight of Fu is respectively as follows: 2000g, 500g, 100g, 100g, 100g, 100g；
(3) the preparation of Herba Dendrobii nutritional solution: by food enzymolysis solution with Herba Dendrobii extracting solution by weight the proportions for 4:1
It is mixed, mixed liquor is put in autoclave, be heated to 90 DEG C, and keep 5min, Herba Dendrobii nutritional solution can be obtained；
(4) fermentation: adding culture fluid in Herba Dendrobii nutritional solution, Herba Dendrobii nutritional solution and culture fluid weight ratio are 10:1, add
Enter lactic acid bacteria, sodium chloride and acetic acid mixed fermentation 1day, add the breast of above-mentioned lactic acid bacteria, sodium chloride and acetic acid each half weight
Acid bacterium, sodium chloride and acetic fermentation, obtain fermentation liquid, lactic acid bacteria, sodium chloride, the weight of total consumption of acetic acid be respectively 120g,
Sodium chloride is 9g, and acetic acid is 4.5g；
The preparation method of culture fluid is: maltose is soluble in water, puts into compound vitamin, after stirring after standing 20min
Heated and boiled, adds Ovum Gallus domesticus album after being cooled to room temperature, stirs, stand-by, maltose, water, compound vitamin, the weight of Ovum Gallus domesticus album
Amount ratio is 100:10000:3:10.
(5) emulsifying: adding emulsifying agent in fermentation liquid, cross leaching clear liquid, after pasteurization, packaging gets product, emulsifying agent is food
With gelatin or carboxymethyl cellulose.
Finished product can cream processed, powder process, be processed further for preparation or the cosmetic material of oral liquid, this course of processing not as
This case inventive point, uses conventional prior art to complete, and in the present invention, each raw material all uses commercial goods.
Step (3) in, after mixed liquor is put into autoclave, add yeast extract 7.5%, dextrinosan 10%-20%,
Citric acid 0.15%-0.25%, sodium citrate 0.05%, L malic acid 0.05%, VC0.03%, it is equipped with food additive: xylose
Alcohol, 0.07%-0.12% xanthan gum 0.5% arabic gum, 0.15% pectin, 0.15% sodium alginate, 0.12%-0.23% carboxymethyl are fine
Dimension element sodium, 0.2%-0.8% fatty acid cane sugar ester emulsifying agent, 0.05% 3 polyglycerin ester, 0.2% Fructus Jujubae essence, beverage flavor agent 0.5%
Ethyl vanillin (bean milk powder essence), food coloring 0.02% phylloxanthin, 1% lecithin (source that Beijing is green, purity is 15%),
Whole or a portion in 0.06% daidzein, black vinegar, polydextrose, nicotinic acid, calcium pantothenate, → allocate, filter →
Sterilizing (mixed liquor being put in autoclave, be heated to 90 DEG C, and keep 5min) → directly beverage can be administered orally by Herba Dendrobii,
Then need not step fermentation (4) and step emulsifying (5), above-mentioned percentage ratio is all each material and food enzymolysis solution and Herba Dendrobii
The percentage ratio of the mixed gross weight of extracting solution.
Embodiment 2
The preparation method of a kind of Herba Dendrobii fermentation liquid, comprises the steps:
Preparation food enzymolysis solution: by etc. the purple rice of weight, Semen setariae, Semen Coicis, Poria, Fructus Jujubae, Fructus Mume, Pericarpium Citri Reticulatae, Fructus Crataegi (parched to brown)
Soak, by food: water is the weight ratio mixing of 1:8, the red bean powder of the 1.5% of the gross weight of addition food and water and material gross weight
1.5% 0.05%NaHCO3, it is allowed to easily to boil, removes raw meat, except bitter taste, after 80 DEG C of 15min that precook, add and adjust with citric acid
PH value 5.5, adds the 0.02% pectase 55 DEG C insulation enzymolysis 2h of the gross weight of food and water, adds food and the gross weight of water
1% hemicellulase, add 0.56% cellulase of the gross weight of food and water, add food and water gross weight 0.5%
0.04% phytase 1h of the gross weight of papain, addition food and water, mixes Fructus Hordei Germinatus, Massa Medicata Fermentata 15h, filters, sterilization,
Stand-by (solid content 8%～10%)；
(2) preparation Herba Dendrobii extracting solution: by Herba Dendrobii water blancing 2min, cooling, by material: water is that the weight ratio of 1:10 adds
Water is pulled an oar, and then mixes with moringa powder, Radix Paeoniae Alba, Fructus Amomi, Rhizoma Polygonati Odorati, Semen Raphani, adds water to 2.5 times of the initial water yield,
Decoct, extract supernatant, stand-by (solid content 4%～6%), Herba Dendrobii, moringa powder, Radix Paeoniae Alba, Fructus Amomi, Rhizoma Polygonati Odorati, Lay
The weight of Fu is respectively as follows: 2500g, 1000g, 150g, 150g, 150g, 150g；
(3) the preparation of Herba Dendrobii nutritional solution: by food enzymolysis solution with Herba Dendrobii extracting solution by weight the proportions for 4:1
It is mixed, mixed liquor is put in autoclave, be heated to 90 DEG C, and keep 5min, Herba Dendrobii nutritional solution can be obtained；
(4) fermentation: adding culture fluid in Herba Dendrobii nutritional solution, Herba Dendrobii nutritional solution and culture fluid weight ratio are 10:1, add
Enter lactic acid bacteria, sodium chloride and acetic acid mixed fermentation 1day, add the breast of above-mentioned lactic acid bacteria, sodium chloride and acetic acid each half weight
Acid bacterium, sodium chloride and acetic fermentation, obtain fermentation liquid, lactic acid bacteria, sodium chloride, the weight of total consumption of acetic acid be respectively 120g,
Sodium chloride is 9g, and acetic acid is 6g；
The preparation method of culture fluid is: maltose is soluble in water, puts into compound vitamin, after stirring after standing 20min
Heated and boiled, adds Ovum Gallus domesticus album after being cooled to room temperature, stirs, stand-by, maltose, water, compound vitamin, the weight of Ovum Gallus domesticus album
Amount ratio is 100:10000:3:10.
(5) emulsifying: adding emulsifying agent in fermentation liquid, cross leaching clear liquid, after pasteurization, packaging gets product, emulsifying agent is food
With gelatin or carboxymethyl cellulose.
Finished product can cream processed, powder process, be processed further for preparation or the cosmetic material of oral liquid, this course of processing not as
This case inventive point, uses conventional prior art to complete, and in the present invention, each raw material all uses commercial goods.
Step (3) in, after mixed liquor is put into autoclave, add yeast extract 7.5%, dextrinosan 10%-20%,
Citric acid 0.15%-0.25%, sodium citrate 0.05%, L malic acid 0.05%, VC0.03%, it is equipped with food additive: xylose
Alcohol, 0.07%-0.12% xanthan gum 0.5% arabic gum, 0.15% pectin, 0.15% sodium alginate, 0.12%-0.23% carboxymethyl are fine
Dimension element sodium, 0.2%-0.8% fatty acid cane sugar ester emulsifying agent, 0.05% 3 polyglycerin ester, 0.2% Fructus Jujubae essence, beverage flavor agent 0.5%
Ethyl vanillin (bean milk powder essence), food coloring 0.02% phylloxanthin, 1% lecithin (source that Beijing is green, purity is 15%),
Whole or a portion in 0.06% daidzein, black vinegar, polydextrose, nicotinic acid, calcium pantothenate, → allocate, filter →
Sterilizing (mixed liquor being put in autoclave, be heated to 90 DEG C, and keep 5min) → directly beverage can be administered orally by Herba Dendrobii,
Then need not step fermentation (4) and step emulsifying (5), above-mentioned percentage ratio is all each material and food enzymolysis solution and Herba Dendrobii
The percentage ratio of the mixed gross weight of extracting solution.
Embodiment 3
The preparation method of a kind of Herba Dendrobii fermentation liquid, comprises the steps:
Preparation food enzymolysis solution: by etc. the purple rice of weight, Semen setariae, Semen Coicis, Poria, Fructus Jujubae, Fructus Mume, Pericarpium Citri Reticulatae, Fructus Crataegi (parched to brown)
Soak, by food: water is the weight ratio mixing of 1:8, the red bean powder of the 1.5% of the gross weight of addition food and water and material gross weight
1.5% 0.05%NaHCO3, it is allowed to easily to boil, removes raw meat, except bitter taste, after 80 DEG C of 15min that precook, add and adjust with citric acid
PH value 5.5, adds the 0.02% pectase 55 DEG C insulation enzymolysis 2h of the gross weight of food and water, adds food and the gross weight of water
1% hemicellulase, add 0.56% cellulase of the gross weight of food and water, add food and water gross weight 0.5%
0.04% phytase 1h of the gross weight of papain, addition food and water, mixes Fructus Hordei Germinatus, Massa Medicata Fermentata 15h, filters, sterilization,
Stand-by (solid content 8%～10%)；
(2) preparation Herba Dendrobii extracting solution: by Herba Dendrobii water blancing 2min, cooling, by material: water is that the weight ratio of 1:10 adds
Water is pulled an oar, and then mixes with moringa powder, Radix Paeoniae Alba, Fructus Amomi, Rhizoma Polygonati Odorati, Semen Raphani, adds water to 2.5 times of the initial water yield,
Decoct, extract supernatant, stand-by (solid content 4%～6%), Herba Dendrobii, moringa powder, Radix Paeoniae Alba, Fructus Amomi, Rhizoma Polygonati Odorati, Lay
The weight of Fu is respectively as follows: 2200g, 1100g, 150g, 120g, 150g, 110g；
(3) the preparation of Herba Dendrobii nutritional solution: by food enzymolysis solution with Herba Dendrobii extracting solution by weight the proportions for 4:1
It is mixed, mixed liquor is put in autoclave, be heated to 90 DEG C, and keep 5min, Herba Dendrobii nutritional solution can be obtained；
(4) fermentation: adding culture fluid in Herba Dendrobii nutritional solution, Herba Dendrobii nutritional solution and culture fluid weight ratio are 10:1, add
Enter lactic acid bacteria, sodium chloride and acetic acid mixed fermentation 1day, add the breast of above-mentioned lactic acid bacteria, sodium chloride and acetic acid each half weight
Acid bacterium, sodium chloride and acetic fermentation, obtain fermentation liquid, lactic acid bacteria, sodium chloride, the weight of total consumption of acetic acid be respectively 120g,
Sodium chloride is 9g, and acetic acid is 6g；
The preparation method of culture fluid is: maltose is soluble in water, puts into compound vitamin, after stirring after standing 20min
Heated and boiled, adds Ovum Gallus domesticus album after being cooled to room temperature, stirs, stand-by, maltose, water, compound vitamin, the weight of Ovum Gallus domesticus album
Amount ratio is 100:10000:3:10.
(5) emulsifying: adding emulsifying agent in fermentation liquid, cross leaching clear liquid, after pasteurization, packaging gets product, emulsifying agent is food
With gelatin or carboxymethyl cellulose.
Finished product can cream processed, powder process, be processed further for preparation or the cosmetic material of oral liquid, this course of processing not as
This case inventive point, uses conventional prior art to complete, and in the present invention, each raw material all uses commercial goods.
Step (3) in, after mixed liquor is put into autoclave, add yeast extract 7.5%, dextrinosan 10%-20%, Fructus Citri Limoniae
Acid 0.15%-0.25%, sodium citrate 0.05%, L malic acid 0.05%, VC0.03%, it is equipped with food additive: xylitol,
0.07%-0.12% xanthan gum 0.5% arabic gum, 0.15% pectin, 0.15% sodium alginate, 0.12%-0.23% carboxymethyl cellulose
Sodium, 0.2%-0.8% fatty acid cane sugar ester emulsifying agent, 0.05% 3 polyglycerin ester, 0.2% Fructus Jujubae essence, beverage flavor agent 0.5% ethyl
Vanillin (bean milk powder essence), food coloring 0.02% phylloxanthin, 1% lecithin (source that Beijing is green, purity is 15%), 0.06%
Whole or a portion in daidzein, black vinegar, polydextrose, nicotinic acid, calcium pantothenate, → allocate, filter → sterilizing (will
Mixed liquor is put in autoclave, is heated to 90 DEG C, and keeps 5min) → directly beverage can be administered orally by Herba Dendrobii, then need not
Step fermentation (4) and step emulsifying (5), above-mentioned percentage ratio is all that each material mixes with food enzymolysis solution and Herba Dendrobii extracting solution
The percentage ratio of the gross weight after conjunction.
Control experiment
Select the healthy male mice 30 with area, body weight 20 ± 2g, be divided into 3 groups at random: matched group (being labeled as A),
Herba Dendrobii fermentation liquid group (being labeled as B) and Herba Dendrobii group (being labeled as C),
The distilled water of A group employing 0.02ml/ (kg d), continuous gavage 10d,
The Herba Dendrobii fermentation liquid that the embodiment of the present invention 1 of B group employing 2.6g/ (kg d) is given, continuous gavage 10d,
The Herba Dendrobii distilled water solution of C group employing 2.6g/ (kg d), continuous gavage 10d,
Adaptability swimming secondary, each 10min is carried out during gavage.
Swimming with a load attached to the body is tested
After last gavage 30min, 3 treated animals all randomly select 5, and the mice of root of the tail portion load 5% body weight sheet lead is placed in trip
Swimming case went swimming.The depth of water is no less than 30cm, water temperature 25 ± 1 DEG C, and record mice swimming time, i.e. mice start from swimming
Upstream face 10s is not had without the time come back to mouth and nose.
Fatigue be body under the conditions of certain environment, because of for a long time the heaviest, the most nervous work (including mental and physical)
A kind of physiological phenomenon of the temporary obvious reduction of work efficiency caused.Fatigue is the result of multifactor effect, the most directly
It is the decline of exercise tolerance with objectively performance, and swimming time is all the time by the important indicator as reflection exercise tolerance.
The mensuration of blood lactase acid
After last medication 30min, often organize remaining 5 animals and gather mouse tail blood immediately, measure blood lactase acid I；Then exist
Adopt tail blood after swimming trunk went swimming (not bearing a heavy burden) 20min immediately, measure blood lactase acid II；After rest 40min, again adopt tail blood,
Measure blood lactase acid III.Assay method is carried out according to the explanation of full blood lactic test kit.
The generation of blood lactase acid and the mensuration of degradation rate
The generating rate of blood lactase acid=(rest value before blood lactase acid peak-motion)/move to time × 100% used by peak；Blood breast
Acid degradation speed=(blood lactase acid value after blood lactase acid peak-rest 40min)/40min × 100%.
Statistical analysis
Data acquisition SPSS11.5 software processes, each experimental group uses single factor test methods analyst to compare two-by-two.
Experimental result
The impact on mice heavy burden swimming time of the Herba Dendrobii fermentation liquid
Herba Dendrobii fermentation liquid the results are shown in Table 1 to the impact of mice heavy burden swimming time.As shown in Table 1, B group mice bears a heavy burden
Swimming time dramatically increases (P ＜ 0.05) than A group and C group, shows that Herba Dendrobii fermentation liquid has obvious anti-fatigue effect.
Table 1 Herba Dendrobii fermentation liquid on the impact of mice heavy burden swimming time (min)
Originally test result indicate that: mice swimming time is had no significant effect by A and the C group that this institute is selected, and iron sheet stone
Dry measure used in former times fermentation liquid can extend the swimming time of mice significantly, and thus prompting Herba Dendrobii fermentation liquid can significantly improve the fortune of mice
Dynamic endurance.
Herba Dendrobii fermentation liquid mice is swum before and after Serum lactic acid content and production of lactic acid and the impact of degradation rate
As shown in Table 2, after each group mouse stomach 10d, before mouse movement, between each group of blood lactase acid value, there is no notable difference.Mice
After carrying out the strenuous exercise of short time (20min), C group and A group do not have difference, B group blood lactase acid value to be all remarkably higher than matched group,
No significant difference between each administration group.
By table 2 it has been also found that after motion rest 40min, compared with A group, the blood lactase acid value (blood lactase acid of B, C group mice
III) all significantly reduce.Although B, C group blood lactase acid generating rate is significantly higher than A group, but the equal C of blood lactase acid degradation rate of B group
Group significantly increases, and the ability of prompting Herba Dendrobii fermentation liquid aerobic metabolism is strengthened.
Table 2 Herba Dendrobii fermentation liquid is on the impact of Serum lactic acid content before and after mice swimming
(mmol/L)
The accumulation of metabolite is that body produces tired main cause, and wherein, the increase with H+ concentration of piling up of lactic acid is important
Factor.Violent physical exertion makes lactic acid pile up in vivo, and blood pH reduces, it is suppressed that phosphofructose swashs the multiple kinds of energy generations such as acid
The activity of enzyme during thanking, makes glycolysis be affected, and ATP supplies minimizing, causes muscle fatigue.After mice swimming 20min,
C group and more blank group of B treated animal Serum lactic acid content substantially increase, and production of lactic acid speed increases the most accordingly, in conjunction with B treated animal
The interpretation of result that swimming time is obviously prolonged, with Herba Dendrobii and enzymatic hydrolysis and fermentation liquid thereof, this promotes that body anaerobic metabolism level has
Close.
After additionally mice swimming stops, 20～50min is convalescent period of blood lactase acid after motion.Convalescent period after exercise, body
Needing a large amount of oxygen of inspiration, now body is in addition to mainly with aerobic metabolism mode energy supply, the oxygen debt in strenuous exercise to be repaid,
The removing of lactic acid completes by aerobic metabolism now just.
Owing to Herba Dendrobii fermentation liquid is in addition to containing sugar, electrolyte, include strengthening possibly together with little molecule small peptide, polymerization sugar, aminoacid
Taurine and abundant plant flavone, vitamin B group, VC and various fatsoluble vitamin, choline, the function such as zinc of high concentration
Composition has great relation.Little molecule small peptide directly can be absorbed by intestinal, energy energy supply rapidly, has accelerated motion muscle fatigue extensive
Multiple effect.
Although disclosing embodiments of the invention for the purpose of illustration, but it will be appreciated by those skilled in the art that: without departing from this
In invention and spirit and scope of the appended claims, various replacements, to change and modifications be all possible, therefore, and the present invention's
Scope is not limited to embodiment disclosure of that.
Claims (6)
1. the preparation method of a Herba Dendrobii fermentation liquid, it is characterised in that: comprise the steps:
Preparation food enzymolysis solution: by etc. the purple rice of weight, Semen setariae, Semen Coicis, Poria, Fructus Jujubae, Fructus Mume, Pericarpium Citri Reticulatae, Fructus Crataegi (parched to brown)
Soak, by food: water is the weight ratio mixing of 1:8, the red bean powder of the 1.5% of the gross weight of addition food and water and material gross weight
1.5% 0.05%NaHCO3, after 80 DEG C of 15min that precook, add and adjust the total of pH value 5.5, addition food and water with citric acid
The 0.02% pectase 55 DEG C insulation enzymolysis 2h of weight, adds 1% hemicellulase of the gross weight of food and water, adds food
With 0.56% cellulase of the gross weight of water, add 0.5% papain of the gross weight of food and water, add food and water
0.04% phytase 1h of gross weight, mix Fructus Hordei Germinatus, Massa Medicata Fermentata 15h, filter, sterilization, obtain food enzymolysis solution, stand-by；
(2) preparation Herba Dendrobii extracting solution: by Herba Dendrobii water blancing 2min, cooling, by material: water is that the weight ratio of 1:10 adds
Water is pulled an oar, and then mixes with moringa powder, Radix Paeoniae Alba, Fructus Amomi, Rhizoma Polygonati Odorati, Semen Raphani, adds water to 2.5 times, decocts, in extraction
Clear liquid, obtains Herba Dendrobii extracting solution, stand-by；
(3) the preparation of Herba Dendrobii nutritional solution: by food enzymolysis solution with Herba Dendrobii extracting solution by weight the proportions for 4:1
It is mixed, mixed liquor is put in autoclave, be heated to 90 DEG C, and keep 5min, Herba Dendrobii nutritional solution can be obtained；
(4) fermentation: adding culture fluid in Herba Dendrobii nutritional solution, Herba Dendrobii nutritional solution and culture fluid weight ratio are 10:1, add
Enter lactic acid bacteria, sodium chloride and acetic acid mixed fermentation 1day, add the breast of above-mentioned lactic acid bacteria, sodium chloride and acetic acid each half weight
Acid bacterium, sodium chloride and acetic fermentation, obtain fermentation liquid；
(5) emulsifying: adding emulsifying agent in fermentation liquid, cross leaching clear liquid, after pasteurization, packaging gets product, emulsifying agent is food
With gelatin or carboxymethyl cellulose.
The preparation method of Herba Dendrobii fermentation liquid the most according to claim 1, it is characterised in that: the food that step is (1) described
The content of the solid content of enzymolysis solution is 8%～10%.
The preparation method of Herba Dendrobii fermentation liquid the most according to claim 1, it is characterised in that: step (2) described in ferrum
The solid content of skin dendrobium extract is 4%～6%.
The preparation method of Herba Dendrobii fermentation liquid the most according to claim 3, it is characterised in that: step (2) described in ferrum
Skin Herba Dendrobii, moringa powder, Radix Paeoniae Alba, Fructus Amomi, Rhizoma Polygonati Odorati, the parts by weight of Semen Raphani be respectively as follows: 20～25 parts, 5～10 parts, 1～2
Part, 1～2 part, 1～2 part, 1～2 part.
The preparation method of Herba Dendrobii fermentation liquid the most according to claim 1, it is characterised in that: the lactic acid that step is (3) described
Bacterium, sodium chloride, the parts by weight of total consumption of acetic acid are respectively 100～120 parts, 8～10 parts, and acetic acid is 4～6 parts.
The preparation method of Herba Dendrobii fermentation liquid the most according to claim 5, it is characterised in that: the cultivation that step is (3) described
The preparation method of liquid is: maltose is soluble in water, puts into compound vitamin after standing 20min, and the post-heating that stirs boils,
Adding Ovum Gallus domesticus album after being cooled to room temperature, stir, stand-by, maltose, water, compound vitamin, the weight ratio of Ovum Gallus domesticus album are
100:10000:3:10。
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|---|---|---|---|---|
| CN111616353A (en) * | 2019-12-31 | 2020-09-04 | 北京中医药大学 | Prunus humilis composite fermentation broth for regulating intestinal flora and preparation method thereof |
| CN115105454A (en) * | 2021-03-19 | 2022-09-27 | 株式会社现代百朗德 | Preparation method of dendrobium officinale flower fermentation product and cosmetic composition containing dendrobium officinale flower fermentation product |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103181520A (en) * | 2013-03-19 | 2013-07-03 | 福建省农业科学院农业生物资源研究所 | Preparation method for dendrobium officinale and lactic acid bacteria capsule |
| CN103385925A (en) * | 2013-07-18 | 2013-11-13 | 中国人民武装警察部队后勤学院 | Fructus cannabis enzymolysis fermentation liquor having fatigue resistance and its preparation method and use |
| CN103564562A (en) * | 2013-07-12 | 2014-02-12 | 宁夏光彩生物科技有限公司 | Fruit and vegetable fermented liquid, fruit and vegetable fermented beverage and preparation method of liquid and beverage |
| CN104585762A (en) * | 2015-01-19 | 2015-05-06 | 中国食品发酵工业研究院 | Dendrobium officinale fermented product and preparation method thereof |
| CN105533389A (en) * | 2016-01-28 | 2016-05-04 | 广西大学 | Dendrobium officinale and carambola fruit solid drink particle and preparation method thereof |
-
2016
- 2016-05-10 CN CN201610309207.6A patent/CN105962368A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103181520A (en) * | 2013-03-19 | 2013-07-03 | 福建省农业科学院农业生物资源研究所 | Preparation method for dendrobium officinale and lactic acid bacteria capsule |
| CN103564562A (en) * | 2013-07-12 | 2014-02-12 | 宁夏光彩生物科技有限公司 | Fruit and vegetable fermented liquid, fruit and vegetable fermented beverage and preparation method of liquid and beverage |
| CN103385925A (en) * | 2013-07-18 | 2013-11-13 | 中国人民武装警察部队后勤学院 | Fructus cannabis enzymolysis fermentation liquor having fatigue resistance and its preparation method and use |
| CN104585762A (en) * | 2015-01-19 | 2015-05-06 | 中国食品发酵工业研究院 | Dendrobium officinale fermented product and preparation method thereof |
| CN105533389A (en) * | 2016-01-28 | 2016-05-04 | 广西大学 | Dendrobium officinale and carambola fruit solid drink particle and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111616353A (en) * | 2019-12-31 | 2020-09-04 | 北京中医药大学 | Prunus humilis composite fermentation broth for regulating intestinal flora and preparation method thereof |
| CN115105454A (en) * | 2021-03-19 | 2022-09-27 | 株式会社现代百朗德 | Preparation method of dendrobium officinale flower fermentation product and cosmetic composition containing dendrobium officinale flower fermentation product |
| CN115105454B (en) * | 2021-03-19 | 2023-09-26 | 株式会社现代百朗德 | Preparation method of dendrobium candidum flower fermentation product and cosmetic composition containing fermentation product |
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