CN105961909A - Stingray feed additive - Google Patents
Stingray feed additive Download PDFInfo
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- CN105961909A CN105961909A CN201610288788.XA CN201610288788A CN105961909A CN 105961909 A CN105961909 A CN 105961909A CN 201610288788 A CN201610288788 A CN 201610288788A CN 105961909 A CN105961909 A CN 105961909A
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- Prior art keywords
- feed additive
- fish feed
- powder
- bifidobacterium bifidum
- stingray
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- 239000003674 animal food additive Substances 0.000 title claims abstract description 39
- 241001670157 Gymnura Species 0.000 title abstract 8
- 239000000843 powder Substances 0.000 claims abstract description 38
- 241000186016 Bifidobacterium bifidum Species 0.000 claims abstract description 37
- 229940002008 bifidobacterium bifidum Drugs 0.000 claims abstract description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000000463 material Substances 0.000 claims abstract description 17
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 14
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 14
- 244000199866 Lactobacillus casei Species 0.000 claims abstract description 14
- 235000013958 Lactobacillus casei Nutrition 0.000 claims abstract description 14
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 14
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 14
- 229940017800 lactobacillus casei Drugs 0.000 claims abstract description 14
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 14
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 13
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 13
- 241000251468 Actinopterygii Species 0.000 claims description 55
- 241000894006 Bacteria Species 0.000 claims description 25
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 22
- 238000002386 leaching Methods 0.000 claims description 22
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 21
- 239000002585 base Substances 0.000 claims description 14
- 235000015099 wheat brans Nutrition 0.000 claims description 14
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 11
- AEMOLEFTQBMNLQ-AQKNRBDQSA-N D-glucopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-AQKNRBDQSA-N 0.000 claims description 11
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 claims description 11
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 11
- 229930006000 Sucrose Natural products 0.000 claims description 11
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 11
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 11
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 11
- 239000006071 cream Substances 0.000 claims description 11
- 229940097043 glucuronic acid Drugs 0.000 claims description 11
- 239000005720 sucrose Substances 0.000 claims description 11
- 239000002671 adjuvant Substances 0.000 claims description 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 239000002245 particle Substances 0.000 claims description 9
- 239000012467 final product Substances 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 7
- 241000209140 Triticum Species 0.000 claims description 6
- 235000021307 Triticum Nutrition 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- 239000003513 alkali Substances 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 230000004913 activation Effects 0.000 claims description 3
- 238000007605 air drying Methods 0.000 claims description 3
- 230000033228 biological regulation Effects 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 230000003068 static effect Effects 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims 4
- 239000007788 liquid Substances 0.000 claims 2
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 239000006041 probiotic Substances 0.000 abstract description 7
- 235000018291 probiotics Nutrition 0.000 abstract description 7
- 235000015097 nutrients Nutrition 0.000 abstract description 6
- 201000010099 disease Diseases 0.000 abstract description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 5
- 244000005709 gut microbiome Species 0.000 abstract description 4
- 239000002253 acid Substances 0.000 abstract 1
- 150000007513 acids Chemical class 0.000 abstract 1
- 229940041514 candida albicans extract Drugs 0.000 abstract 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 239000012138 yeast extract Substances 0.000 abstract 1
- 230000000529 probiotic effect Effects 0.000 description 5
- 235000016709 nutrition Nutrition 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 210000001035 gastrointestinal tract Anatomy 0.000 description 3
- 230000000968 intestinal effect Effects 0.000 description 3
- 235000019733 Fish meal Nutrition 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000005538 encapsulation Methods 0.000 description 2
- 239000004467 fishmeal Substances 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 208000000412 Avitaminosis Diseases 0.000 description 1
- 241000251730 Chondrichthyes Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 206010021135 Hypovitaminosis Diseases 0.000 description 1
- 241000722085 Synanceia horrida Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 208000030401 vitamin deficiency disease Diseases 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/517—Bifidum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Fodder In General (AREA)
Abstract
The present invention discloses a stingray feed additive. The stingray feed additive consists of the following components in weight percentages: 20-30% of bacillus subtilis, 9-12% of lactobacillus casei, 8-13% of lactobacillus plantarum, 8-11% of lactobacillus acidophilus, 4-6% of bifidobacterium bifidum, 15-25% of yeast extract powder, 9-11% of glucuronic acids and 5-15% of accessory materials. A certain amount of probiotics are added to maintain the balance of the gut microflora of the stingray, inhibit the growth of the harmful microorganisms in the water body, and promote the establishment of the nitrification system in the water body. At the same time, a certain proportion of the nutrient components are added to provide the nutrients needed for the growth of the stingray on the other hand, and are with the probiotics to maintain the balance of the gut microflora of the stingray, reduce the incidences of diseases of the stingray, and be conducive to the normal growth of the stingray.
Description
Technical field
The present invention relates to feed additive technology, especially relate to a kind of fish feed additive.
Background technology
Fish also known as devil fish, is similar in the shark occurred in Mesozoic Jurassic Period (before about 1.8 hundred million years～1.4 hundred million years).In pet fish, fish is a kind of rare cartilage freshwater fish, because seemingly dancing lightly when it moves about in water, and is deeply liked by vast fish friend.The toys such as fish is predacious fish, fish, shrimp and the worm in predation water.The fish of artificial feed, because recipe is single, easily causes the problems such as vitamin deficiency, intestinal flora imbalance, and then causes various disease and death.
At present, occur in that fish desired nutritional element in fish meal, but be easily caused fish and occur because of nutrient imbalance, intestinal tract disease, be unfavorable for setting up and the normal growth of fish of nitrification in fish and water body.
Summary of the invention
It is an object of the invention to overcome above-mentioned technical deficiency, propose a kind of fish feed additive, solve in prior art in fish feeding process because feedstuff defect causes the technical problem that nitrification in fish and water body is unbalance, fish mortality rate raises.
For reaching above-mentioned technical purpose, technical scheme provides a kind of fish feed additive, including following components in weight percent: 20～30% bacillus subtilis, 9～12% lactobacillus casei, 8～13% Lactobacillus plantarum, 8～11% bacillus acidophilus, 4～6% bifidobacterium bifidum, 15～25% yeast leaching powder, 9～11% glucuronic acid, 5～15% adjuvant.
Preferably, including following components in weight percent: 25% bacillus subtilis, 10% lactobacillus casei, 10% Lactobacillus plantarum, 10% bacillus acidophilus, 5% bifidobacterium bifidum, 20% yeast leaching powder, 10% glucuronic acid, 10% adjuvant.
Preferably, effective bacterium number of described bacillus subtilis is not less than 1 × 1010Individual/g, described lactobacillus casei, Lactobacillus plantarum, effective bacterium number of bacillus acidophilus are all not less than 1 × 108Individual/g, effective bacterium number of described bifidobacterium bifidum is not less than 5 × 107Individual/g.
Preferably, described bifidobacterium bifidum is adopted and is cultivated with the following method:
(1) Testa Tritici is pulverized and sieved, choose the fine powder that wherein particle diameter is more than 100 mesh and the coarse powder that particle diameter is 20～50 mesh, fine powder and coarse powder are uniformly mixed for 3:1 in mass ratio, obtains Testa Tritici base material；
(2) Testa Tritici base material step (1) prepared adds sucrose, maltose, oligofructose, oligomeric xylose, yeast leaching powder, Carnis Bovis seu Bubali cream, uniformly add after mixing appropriate distilled water stirring to water content be 45～60%, pH be the wheat bran medium of 6.8～7.0；
(3) take wheat bran medium inoculation bifidobacterium bifidum seed liquor, load in valve bag after being uniformly mixed, be discharged from envelope inner air seal, and ferment in 37 DEG C of incubators
(4) every 12 hours, in valve bag, add alkali liquor regulation pH to 6.5～7.0, ferment 48 hours, take out culture；
(5) being pulverized and sieved by wheat stalk, the wheat stalk powder taking more than particle diameter 200 mesh uniformly mixes with mass ratio 1:3 with culture, and forced air drying at 45 DEG C, is less than 10% to mixture water content, to obtain final product.
Preferably, sterilizing 20 minutes in step (2) also includes being placed in wheat bran medium the sealing container of 115 DEG C.
Preferably, in described step (2), each component is by weight: Testa Tritici base material 80～120 parts, sucrose 0.3～0.7 part, maltose 0.4～0.6 part, oligofructose 0.05～0.2 part, oligomeric xylose 0.05～0.2 part, yeast leaching powder 0.05～0.2 part, Carnis Bovis seu Bubali cream 0.1～0.3 part.
Preferably, in described step (2), each component is by weight: Testa Tritici base material 100 parts, sucrose 0.5 part, maltose 0.5 part, oligofructose 0.1 part, oligomeric xylose 0.1 part, yeast leaching 0.1 part of powder, Carnis Bovis seu Bubali cream 0.15 part.
Preferably, described bifidobacterium bifidum seed liquor preparation method is: activated in MRS solid plate by bifidobacterium bifidum picking in glycerol pipe, single bacterium of picking activation in MRS fluid medium, static gas wave refrigerator 24 hours and get final product at 37 DEG C.
Preferably, in described step (3), every 100 mass parts wheat bran medium inoculate 20 parts by volume bifidobacterium bifidum seed liquor；Wherein, mass parts and the relation that relation is g and ml of parts by volume.
Preferably, the sodium hydroxide solution that alkali liquor is 2.5mol/L in described step (4).
Compared with prior art, the present invention maintains fish intestinal microflora to balance by the probiotic bacteria of a certain amount of proportioning, harmful microbe growth in suppression water body, promote the foundation of nitrification in water body, adding the nutritional labeling of a certain amount of proportioning, on the one hand it provide the nutrient of fish growth needs simultaneously, on the other hand coordinates maintenance fish intestinal microbial population balance with probiotic bacteria, reduce fish incidence probability, beneficially fish normal growth.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Embodiment
1
:
Embodiments of the invention 1 provide a kind of fish feed additive, following components in weight percent can uniformly be mixed and get final product, concrete component is: 25% bacillus subtilis, 10% lactobacillus casei, 10% Lactobacillus plantarum, 10% bacillus acidophilus, 5% bifidobacterium bifidum, 20% yeast leaching powder, 10% glucuronic acid, 10% adjuvant.Wherein, yeast leaching powder and glucuronic acid are both preferably food stage.
In order to ensure fish feed additive that the present embodiment 1 prepares better substantially in the present embodiment 1, effective bacterium number of probiotic bacteria must reach some, specifically, effective bacterium number of described bacillus subtilis is not less than 1 × 1010Individual/g, described lactobacillus casei, Lactobacillus plantarum, effective bacterium number of bacillus acidophilus are all not less than 1 × 108Individual/g, effective bacterium number of described bifidobacterium bifidum is not less than 5 × 107Individual/g.
Wherein, in order to avoid effective bacterium number of bifidobacterium bifidum is relatively low and affect the overall efficacy of fish feed additive, the described bifidobacterium bifidum of the present embodiment preferably employs following method and cultivates:
(1) Testa Tritici is pulverized and sieved, choose the fine powder that wherein particle diameter is more than 100 mesh and the coarse powder that particle diameter is 20～50 mesh, fine powder and coarse powder are uniformly mixed for 3:1 in mass ratio, obtains Testa Tritici base material；
(2) every 100g Testa Tritici base material step (1) prepared adds sucrose 0.5g, maltose 0.5g, oligofructose 0.1g, oligomeric xylose 0.1g, yeast leaching powder 0.1g, Carnis Bovis seu Bubali cream 0.15g, uniformly add after mixing appropriate distilled water stirring to water content be 45～60%, pH be the wheat bran medium of 6.8～7.0, then wheat bran medium is placed in sterilizing 20 minutes in the sealing container of 115 DEG C, cools down stand-by；
(3) take inoculation bifidobacterium bifidum seed liquor 5ml of wheat bran medium 100g after above-mentioned cooling, load in valve bag after being uniformly mixed, be discharged from envelope inner air seal, and ferment in 37 DEG C of incubators；During encapsulation, general encapsulation material 40～about 45% in valve bag, in order to extruding valve bag discharges air in bag；Wherein, bifidobacterium bifidum seed liquor preparation method is: activated in MRS solid plate by bifidobacterium bifidum picking in glycerol pipe, single bacterium of picking activation in MRS fluid medium, static gas wave refrigerator 24 hours and get final product at 37 DEG C；
(4), in sweat, in valve bag, added the sodium hydroxide solution regulation pH to 6.5～7.0 of 2.5mol/L every 12 hours, ferment 48 hours, take out culture；
(5) being pulverized and sieved by dry wheat stalk, the wheat stalk powder taking more than particle diameter 200 mesh uniformly mixes with mass ratio 1:3 with culture, and forced air drying at 45 DEG C, is less than 10% to mixture water content, to obtain final product.
Embodiment
2
:
Embodiments of the invention 2 provide a kind of fish feed additive, it is substantially the same manner as Example 1, difference is, the concrete component of the fish feed additive of the present embodiment 2 is: 20% bacillus subtilis, 9% lactobacillus casei, 11% Lactobacillus plantarum, 8% bacillus acidophilus, 4% bifidobacterium bifidum, 22% yeast leaching powder, 11% glucuronic acid, 15% adjuvant.
Embodiment
3
:
Embodiments of the invention 3 provide a kind of fish feed additive, it is substantially the same manner as Example 1, difference is, the concrete component of the fish feed additive of the present embodiment 3 is: 30% bacillus subtilis, 11% lactobacillus casei, 8% Lactobacillus plantarum, 10% bacillus acidophilus, 5% bifidobacterium bifidum, 16% yeast leaching powder, 9% glucuronic acid, 11% adjuvant.
Embodiment
4
:
Embodiments of the invention 4 provide a kind of fish feed additive, it is substantially the same manner as Example 1, difference is, the concrete component of the fish feed additive of the present embodiment 4 is: 22% bacillus subtilis, 12% lactobacillus casei, 13% Lactobacillus plantarum, 9% bacillus acidophilus, 6% bifidobacterium bifidum, 15% yeast leaching powder, 11% glucuronic acid, 12% adjuvant.
Embodiment
5
:
Embodiments of the invention 5 provide a kind of fish feed additive, it is substantially the same manner as Example 1, difference is, the concrete component of the fish feed additive of the present embodiment 5 is: 27% bacillus subtilis, 10% lactobacillus casei, 11% Lactobacillus plantarum, 10% bacillus acidophilus, 4% bifidobacterium bifidum, 21% yeast leaching powder, 9% glucuronic acid, 8% adjuvant.
Embodiment
6
:
Embodiments of the invention 6 provide a kind of fish feed additive, it is substantially the same manner as Example 1, difference is, the concrete component of the fish feed additive of the present embodiment 6 is: 25% bacillus subtilis, 11% lactobacillus casei, 12% Lactobacillus plantarum, 9% bacillus acidophilus, 6% bifidobacterium bifidum, 21% yeast leaching powder, 11% glucuronic acid, 5% adjuvant.
Embodiment
7
:
Embodiments of the invention 7 provide a kind of fish feed additive, it is substantially the same manner as Example 1, difference is, in the incubation of the bifidobacterium bifidum of the present embodiment 7, the concrete component of wheat bran medium is: Testa Tritici base material 80g, sucrose 0.6g, maltose 0.4g, oligofructose 0.2g, oligomeric xylose 0.08g, yeast leaching powder 0.15g, Carnis Bovis seu Bubali cream 0.1g.
Embodiment
8
:
Embodiments of the invention 8 provide a kind of fish feed additive, it is substantially the same manner as Example 1, difference is, in the preparation process of the bifidobacterium bifidum of the present embodiment 8, the concrete component of wheat bran medium is: Testa Tritici base material 120g, sucrose 0.3g, maltose 0.6g, oligofructose 0.05g, oligomeric xylose 0.15g, yeast leaching powder 0.06g, Carnis Bovis seu Bubali cream 0.3g.
Embodiment
9
:
Embodiments of the invention 9 provide a kind of fish feed additive, it is substantially the same manner as Example 1, difference is, in the preparation process of the bifidobacterium bifidum of the present embodiment 7, the concrete component of wheat bran medium is: Testa Tritici base material 112g, sucrose 0.5g, maltose 0.5g, oligofructose 0.12g, oligomeric xylose 0.09g, yeast leaching powder 0.1g, Carnis Bovis seu Bubali cream 0.2g.
Embodiment
10
:
Embodiments of the invention 10 provide a kind of fish feed additive, it is substantially the same manner as Example 1, difference is, in the preparation process of the bifidobacterium bifidum of the present embodiment 10, the concrete component of wheat bran medium is: Testa Tritici base material 95g, sucrose 0.4g, maltose 0.4g, oligofructose 0.08g, oligomeric xylose 0.2g, yeast leaching powder 0.2g, Carnis Bovis seu Bubali cream 0.1g.
Experimental example 1
In order to contrast the fish feed additive effect of the present embodiment 1～6, commercially conventional fish meal, it is divided into eight parts, six parts are wherein separately added into the fish feed additive of the present embodiment 1～6 preparation, fish desired nutritional element, the other portion bought on a addition market in other two parts are added without any material, raise fish the most respectively, after one month, statistical data, table specific as follows:
| Reduced parameter | Intestinal tract disease incidence rate | Mortality rate |
| Embodiment 1 | 20% | 3% |
| Embodiment 2 | 22% | 4% |
| Embodiment 3 | 25% | 5% |
| Embodiment 4 | 21% | 4% |
| Embodiment 5 | 25% | 6% |
| Embodiment 6 | 26% | 5% |
| Comparative example 1 (addition nutrient) | 70% | 60% |
| Comparative example 2 (no additives) | 75% | 65% |
As seen from the above table, the fish feed additive of the present embodiment 1～6 substantially can reduce the intestinal tract disease incidence rate of fish, very big reduces the mortality rate that fish is raised.
Experimental example 2
Effect due to effective bacterium number meeting large effect fish feed additive of bifidobacterium bifidum, and effective bacterium number that the cultural method of existing bifidobacterium bifidum is easily caused bifidobacterium bifidum is relatively low, therefore the effective bacterium number in the bifidobacterium bifidum bifidobacterium bifidum of the cultivation of the present embodiment 1 and 7～10 and existing conventional method cultivated contrasts, concrete outcome see table:
As seen from the above table, the present embodiment 1 and 7～10 is compared conventional culture methods and is significantly improved effective bacterium number of bifidobacterium bifidum, the most preferably improves the survival rate of bifidobacterium bifidum.
The present invention maintains fish intestinal microflora to balance by the probiotic bacteria of a certain amount of proportioning, harmful microbe growth in suppression water body, promote the foundation of nitrification in water body, add the nutritional labeling of a certain amount of proportioning simultaneously, on the one hand it provide the nutrient of fish growth needs, on the other hand coordinate maintenance fish intestinal microbial population balance with probiotic bacteria, reduce fish incidence probability, beneficially fish normal growth.
The detailed description of the invention of present invention described above, is not intended that limiting the scope of the present invention.Any technology according to the present invention is conceived various other made and is changed accordingly and deformation, should be included in the protection domain of the claims in the present invention.
Claims (10)
1. a fish feed additive, it is characterised in that include following components in weight percent: 20～30% hay bud
Spore bacillus, 9～12% lactobacillus casei, 8～13% Lactobacillus plantarum, 8～11% bacillus acidophilus, 4～6% not tally bifid bar
Bacterium, 15～25% yeast leaching powder, 9～11% glucuronic acid, 5～15% adjuvant.
Fish feed additive the most according to claim 1, it is characterised in that include following percentage by weight group
Part: 25% bacillus subtilis, 10% lactobacillus casei, 10% Lactobacillus plantarum, 10% bacillus acidophilus, 5% not tally
Bacillus bifidus, 20% yeast leaching powder, 10% glucuronic acid, 10% adjuvant.
Fish feed additive the most according to claim 1, it is characterised in that having of described bacillus subtilis
Effect bacterium number is not less than 1 × 1010Individual/g, described lactobacillus casei, Lactobacillus plantarum, bacillus acidophilus effective bacterium number the most not
Less than 1 × 108Individual/g, effective bacterium number of described bifidobacterium bifidum is not less than 5 × 107Individual/g.
4. according to the arbitrary described fish feed additive of claims 1 to 3, it is characterised in that described not tally bifid bar
Bacterium is adopted and cultivates with the following method:
(1) Testa Tritici is pulverized and sieved, choose fine powder that wherein particle diameter is more than 100 mesh and particle diameter is that 20～50 purposes are thick
Powder, uniformly mixes for 3:1 in mass ratio by fine powder and coarse powder, obtains Testa Tritici base material；
(2) Testa Tritici base material step (1) prepared adds sucrose, maltose, oligofructose, oligomeric xylose, ferment
Female leaching powder, Carnis Bovis seu Bubali cream, uniformly add after mixing appropriate distilled water stirring to water content be 45～60%, pH be 6.8～7.0
Wheat bran medium；
(3) take wheat bran medium inoculation bifidobacterium bifidum seed liquor, load after being uniformly mixed in valve bag, row
Go out valve bag inner air seal, and ferment in 37 DEG C of incubators
(4) every 12 hours, in valve bag, add alkali liquor regulation pH to 6.5～7.0, ferment 48 hours, take out
Culture；
(5) wheat stalk is pulverized and sieved, take the wheat stalk powder of more than particle diameter 200 mesh with culture with mass ratio 1:3
Uniformly mixing, and forced air drying at 45 DEG C, is less than 10% to mixture water content, to obtain final product.
Fish feed additive the most according to claim 4, it is characterised in that step (2) also includes Testa Tritici
Culture medium is placed in sterilizing 20 minutes in the sealing container of 115 DEG C.
Fish feed additive the most according to claim 4, it is characterised in that each component in described step (2)
By weight: Testa Tritici base material 80～120 parts, sucrose 0.3～0.7 part, maltose 0.4～0.6 part, oligofructose 0.05～0.2
Part, oligomeric xylose 0.05～0.2 part, yeast leaching powder 0.05～0.2 part, Carnis Bovis seu Bubali cream 0.1～0.3 part.
Fish feed additive the most according to claim 6, it is characterised in that each component in described step (2)
By weight: Testa Tritici base material 100 parts, sucrose 0.5 part, maltose 0.5 part, oligofructose 0.1 part, oligomeric xylose
0.1 part, yeast leaching 0.1 part of powder, Carnis Bovis seu Bubali cream 0.15 part.
Fish feed additive the most according to claim 4, it is characterised in that described bifidobacterium bifidum seed
Liquid and preparation method thereof is: activated to MRS solid plate by bifidobacterium bifidum picking in glycerol pipe, picking activation
Single bacterium in MRS fluid medium, static gas wave refrigerator 24 hours and get final product at 37 DEG C.
Fish feed additive the most according to claim 4, it is characterised in that in described step (3) every 100
Mass parts wheat bran medium inoculates 20 parts by volume bifidobacterium bifidum seed liquor；Wherein, mass parts and the relation of parts by volume
Relation for g Yu ml.
Fish feed additive the most according to claim 4, it is characterised in that the alkali in described step (4)
Liquid is the sodium hydroxide solution of 2.5mol/L.
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| CN201610288788.XA CN105961909A (en) | 2016-05-04 | 2016-05-04 | Stingray feed additive |
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| CN201610288788.XA CN105961909A (en) | 2016-05-04 | 2016-05-04 | Stingray feed additive |
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| CN107568503A (en) * | 2017-08-29 | 2018-01-12 | 安徽鸿运生态农业有限公司 | A kind of chub feed |
| CN107568502A (en) * | 2017-08-29 | 2018-01-12 | 安徽鸿运生态农业有限公司 | A kind of anti-oxidant antibacterial fish meal |
| CN107988101A (en) * | 2017-12-06 | 2018-05-04 | 广东白鹤生物科技发展有限公司 | A kind of complex microorganism preparations and preparation method thereof |
| CN109362946A (en) * | 2018-09-14 | 2019-02-22 | 广东渔夫宝水产科技有限公司 | A kind of viable bacteria pool head fermentation preparation and its application method for fishes and shrimps intestinal health |
| CN110122381A (en) * | 2019-06-24 | 2019-08-16 | 北京市水产科学研究所（国家淡水渔业工程技术研究中心） | A kind of mating system of ray fish |
| CN111802533A (en) * | 2020-06-17 | 2020-10-23 | 武汉市英牌科技发展有限公司 | Formula for conditioning intestinal tracts of fishes and increasing microelements required by field fishes |
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| CN111802533A (en) * | 2020-06-17 | 2020-10-23 | 武汉市英牌科技发展有限公司 | Formula for conditioning intestinal tracts of fishes and increasing microelements required by field fishes |
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