CN105961459B - For the composition and its preparation of the prevention and control of plant diseases, pest control, preparation method and application - Google Patents
For the composition and its preparation of the prevention and control of plant diseases, pest control, preparation method and application Download PDFInfo
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- CN105961459B CN105961459B CN201610406995.0A CN201610406995A CN105961459B CN 105961459 B CN105961459 B CN 105961459B CN 201610406995 A CN201610406995 A CN 201610406995A CN 105961459 B CN105961459 B CN 105961459B
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 36
- 201000010099 disease Diseases 0.000 title claims abstract description 35
- 239000000203 mixture Substances 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 33
- 230000002265 prevention Effects 0.000 title claims abstract description 19
- 241000607479 Yersinia pestis Species 0.000 title abstract description 22
- 241000208340 Araliaceae Species 0.000 claims abstract description 46
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 45
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 43
- 235000008434 ginseng Nutrition 0.000 claims abstract description 43
- 241000758794 Asarum Species 0.000 claims abstract description 18
- 241001465251 Ephedra sinica Species 0.000 claims abstract description 18
- 241000205407 Polygonum Species 0.000 claims abstract description 18
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 claims abstract description 18
- 241001504477 Pycnonotidae Species 0.000 claims abstract description 14
- 239000000839 emulsion Substances 0.000 claims abstract description 10
- 235000008658 Artemisia capillaris Nutrition 0.000 claims abstract description 3
- 241000092668 Artemisia capillaris Species 0.000 claims abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Natural products CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 25
- 238000000034 method Methods 0.000 claims description 22
- 239000000284 extract Substances 0.000 claims description 21
- 241000245665 Taraxacum Species 0.000 claims description 17
- 235000003092 Artemisia dracunculus Nutrition 0.000 claims description 15
- 240000001851 Artemisia dracunculus Species 0.000 claims description 15
- 239000000341 volatile oil Substances 0.000 claims description 14
- 235000019441 ethanol Nutrition 0.000 claims description 13
- 239000000706 filtrate Substances 0.000 claims description 12
- 238000000605 extraction Methods 0.000 claims description 11
- 239000003995 emulsifying agent Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 239000002357 osmotic agent Substances 0.000 claims description 8
- 230000002829 reductive effect Effects 0.000 claims description 8
- 238000010992 reflux Methods 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 8
- 239000012752 auxiliary agent Substances 0.000 claims description 7
- 239000003960 organic solvent Substances 0.000 claims description 7
- 206010027146 Melanoderma Diseases 0.000 claims description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 6
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims description 6
- AXTGDCSMTYGJND-UHFFFAOYSA-N 1-dodecylazepan-2-one Chemical group CCCCCCCCCCCCN1CCCCCC1=O AXTGDCSMTYGJND-UHFFFAOYSA-N 0.000 claims description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 5
- 239000006184 cosolvent Substances 0.000 claims description 5
- 239000001509 sodium citrate Substances 0.000 claims description 5
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical group O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 5
- 239000006071 cream Substances 0.000 claims description 4
- 239000003921 oil Substances 0.000 claims description 4
- 241000918585 Pythium aphanidermatum Species 0.000 claims description 3
- 210000000481 breast Anatomy 0.000 claims description 3
- 230000006837 decompression Effects 0.000 claims description 3
- 241000180649 Panax notoginseng Species 0.000 claims description 2
- 235000003143 Panax notoginseng Nutrition 0.000 claims description 2
- 240000005373 Panax quinquefolius Species 0.000 claims description 2
- 239000002552 dosage form Substances 0.000 claims description 2
- 125000005909 ethyl alcohol group Chemical group 0.000 claims description 2
- 239000011259 mixed solution Substances 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 238000001256 steam distillation Methods 0.000 claims description 2
- 208000004842 Pinta Diseases 0.000 claims 1
- 230000009514 concussion Effects 0.000 claims 1
- 208000011079 pinta disease Diseases 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 22
- 239000000126 substance Substances 0.000 abstract description 4
- 240000001949 Taraxacum officinale Species 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 239000003814 drug Substances 0.000 description 12
- 230000006378 damage Effects 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 238000001914 filtration Methods 0.000 description 7
- 238000003756 stirring Methods 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 6
- 244000052769 pathogen Species 0.000 description 6
- 230000001717 pathogenic effect Effects 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 230000000844 anti-bacterial effect Effects 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 241000233866 Fungi Species 0.000 description 4
- 241000813090 Rhizoctonia solani Species 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 239000000575 pesticide Substances 0.000 description 4
- 239000001965 potato dextrose agar Substances 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000000227 grinding Methods 0.000 description 3
- 239000002917 insecticide Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000001018 virulence Effects 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000010534 mechanism of action Effects 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 239000006013 carbendazim Substances 0.000 description 1
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013065 commercial product Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 244000000011 human parasite Species 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 239000003002 pH adjusting agent Substances 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000012876 topography Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Medicines Containing Plant Substances (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to prevention and control of plant diseases, pest control field, a kind of composition for the ginseng prevention and control of plant diseases, pest control and its preparation, preparation method and application are specifically provided；Composition of the present invention for the ginseng prevention and control of plant diseases, pest control is composed of the following components in parts by weight: 0.1~1.0 part of polygonum cuspidate, 1~5 part of asarum, 1~5 part of the Chinese bulbul, 1~5 part of kuh-seng, 1~6 part of Artemisia capillaris, 0.5~2 part of Herba Andrographitis, 1~5 part of rhizoma acori graminei, 0.1~0.5 part and 0.5~3 part of dandelion of Chinese ephedra；The preparation of composition of the present invention is emulsion, missible oil or pulvis, and the present composition does not add any chemicals ingredient, and there is no chemical residuals to destroy ecology after use, while also improving the yield and quality of ginseng.
Description
Technical field
The present invention relates to plant pest management technical fields, and in particular to a kind of disease pest for during ginseng planting
The composition and its preparation, preparation method and application of evil prevention and treatment.
Background technique
Ginseng is China's rare Chinese medicine, but due to the distinctive growth characteristic of ginseng, meets with it often in growth and development process
Infected by various disease fungus, according to statistics, in China the production loss due to caused by various diseases every year be up to 20%~
50%.Disease fungus is the major obstacle of ginseng stable yields, directly affects yield and commodity value, seriously weakens China's ginseng
The competitiveness of product in the international market, causes heavy economic losses.And ginseng diseases are many kinds of, and only China just has 20
A variety of, generation is universal in planting process, harm is serious mainly damping-off, black spot, rust rot, epidemic disease etc..
The current main prevention and treatment that pest and disease damage is carried out using chemical bactericide, but its duration of efficacy is short, residue problem
It is prominent, very big harm is caused to human health and ecological environment, such as by taking the method sprayed insecticide.Such as the
No. 201110439071 Chinese patent applications disclose a kind of pesticide administering method for preventing diseases of ginseng pesticide administering method for preventing diseases of ginseng ", it is logical
The method sprayed insecticide is crossed to achieve the effect that prevention and treatment, but there are soil to have pesticide residue, influences lacking for the quality of ginseng
It falls into.
Or carrys out pre- preventing disease and pest by adjusting ginseng planting's condition and occur, such as No. 201310101875 Chinese patent Shen
It please disclose a kind of " method of prevention and treatment black fleck disease of ginseng ", this method prevents ginseng blackspot by adjusting the planting conditions of ginseng
Disease, but party's law limitation be black spot disease hair when, can not but play the role for the treatment of.
Applying for medicinal plant has long history in China, has to obtain day in the research that China carries out botanical pesticide
Solely thick advantage.Plant generates a variety of secondary metabolites during long-term evolution to resist external pest and disease damage.Medicinal plant
In have many types to human parasite, human bacterial, fungi, virus have killing or inhibiting effect.
It is chosen in clearing heat and detoxicating, alleviating rheumatism and traditional medicinal plant of expelling parasite, desinsection and other effects according to herbal pharmacology
Screening has antibacterial, bactericidal effect plant, studies their activity to agricultural pest, develops a kind of new plant system
Agent is applied to the prevention and control of plant diseases, pest control of crops, not only can be well protected ecological environment, moreover it is possible to improve the quality of crops.
Therefore develop a kind of new Chinese medicine composition that can effectively prevent ginseng pest and disease damage, then to the plantation of ginseng with
And the protection of ecological environment is all significant.
Summary of the invention
For the above state of the art, the invention proposes the diseases prevented and treated during ginseng planting with Chinese medicine composition, and
Prevention and treatment of the ginseng planting in the process to pest and disease damage are realized simultaneously.Composition of the present invention is composed of the following components
(in parts by weight): 0.1~1.0 part of polygonum cuspidate, 1~5 part of asarum, 1~5 part of the Chinese bulbul, 1~5 part of kuh-seng, 1~6 part of Artemisia capillaris,
0.5~2 part of Herba Andrographitis, 1~5 part of rhizoma acori graminei, 0.1~0.5 part and 0.5~3 part of dandelion of Chinese ephedra.
It is composed of the following components as one of embodiment of the present invention, the composition: 0.5~0.8 part of polygonum cuspidate, asarum 2
~4 parts, 2~4 parts of the Chinese bulbul, 2~4 parts of kuh-seng, 2~5 parts of Tarragon, 0.8~1.8 part of Herba Andrographitis, 2~4 parts of rhizoma acori graminei, Chinese ephedra
0.2~0.4 part and 0.8~2 part of dandelion.
It is composed of the following components as one of embodiment of the present invention, the composition: 0.7 part of polygonum cuspidate, 3 parts of asarum, white
3 parts of father-in-law of head, 3 parts of kuh-seng, 4 parts of Tarragon, 1 part of Herba Andrographitis, 3 parts of rhizoma acori graminei, 0.3 part of Chinese ephedra and 1.5 parts of dandelion.
It is composed of the following components as one of embodiment of the present invention, the composition: 1 part of polygonum cuspidate, 5 parts of asarum, hoary hair
5 parts of father-in-law, 5 parts of kuh-seng, 6 parts of Tarragon, 2 parts of Herba Andrographitis, 5 parts of rhizoma acori graminei, 0.5 part of Chinese ephedra and 3 parts of dandelion.
It is composed of the following components as one of embodiment of the present invention, the composition: 0.1 part of polygonum cuspidate, 1 part of asarum, white
1 part of father-in-law of head, 1 part of kuh-seng, 1 part of Tarragon, 0.5 part of Herba Andrographitis, 1 part of rhizoma acori graminei, 0.1 part of Chinese ephedra and 0.5 part of dandelion.
It is composed of the following components as one of embodiment of the present invention, the composition: 0.5 part of polygonum cuspidate, 3 parts of asarum, white
3 parts of father-in-law of head, 3 parts of kuh-seng, 4 parts of Tarragon, 1.3 parts of Herba Andrographitis, 3 parts of rhizoma acori graminei, 0.3 part of Chinese ephedra and 1.5 parts of dandelion.
For the preparation method of the drug composition of the ginseng prevention and control of plant diseases, pest control, there is no particular limitation in the present invention, can be by this
Field technical staff combines the content of present invention and common sense in the field to prepare, such as can be by various drug components according to phase
Then directly powder is subject to using can also be by the extraction process of this field routine to the present composition for the parts by weight answered
In each component extract mixed after effective component prepared after carry out using.
Various be suitable in ginseng can also be made using conventional formulation technologies in the present composition by those skilled in the art
The preparation of the prevention and control of plant diseases, pest control in planting process.
As one of embodiment, the present invention includes but is not limited to cream for the preparation of the composition of the ginseng prevention and control of plant diseases, pest control
Agent, missible oil or pulvis.
As one of embodiment, for the preparation containing the present composition also containing 1~40 part of auxiliary agent, the auxiliary agent can
Think the various auxiliary agents suitable for different dosage forms of this field routinely, the present invention include but is not limited to be selected from emulsifier, cosolvent,
High osmotic agent or they two or more composition.
As one of embodiment, the emulsifier includes but is not limited to that emulsifier is selected from agriculture breast 300, NP-21 or peaceful cream
No. 32, above-mentioned code name product is commercial product, and those skilled in the art can also equally prepare according to conventional method in that art
The amount of corresponding emulsifier, the emulsifier can combine the present invention and common sense in the field to be determined by skilled person.
As one of embodiment of the present invention, the high osmotic agent includes but is not limited to azone, and the amount of the high osmotic agent can be with
Skilled person combines the present invention and common sense in the field to be determined；
As one of embodiment of the present invention, institute's cosolvent is ethyl alcohol, and concentration is concentration commonly used in the art.
As one of embodiment of the present invention, the pH value of the preparation is 2~9, as one of embodiment, preferably 3
~7；Those skilled in the art can adjust pH value within the above range using conventional method, as one of embodiment, this hair
It is bright that pH value is adjusted as pH adjusting agent using including but not limited to sodium citrate.
It can adopt and be prepared the various preparations containing the present composition with the conventional methods in the field, as this hair
One of bright embodiment, it is preferred to use prepared by following method, including but not limited to the following steps:
1) asarum and rhizoma acori graminei are weighed, using extraction by steam distillation volatile oil；
2) polygonum cuspidate, Tarragon, Herba Andrographitis and Chinese ephedra are weighed, the organic solvent of 10~20 times of amounts is added, impregnates 24~48h,
Period is primary every stirring in 3 hours；It extracts 2~3 times, decompression filters, and obtains filtrate and residue, filtrate is concentrated under reduced pressure, water-bath
Temperature is 50~80 DEG C；Being concentrated into mass concentration is 2g/mL；
3) Chinese bulbul, kuh-seng and dandelion are weighed, 10~20 times of water is added, refluxing extraction 4~8 hours, extracts 2~3
Secondary, filtering, filtrate is concentrated under reduced pressure, and bath temperature is 50~80 DEG C, and being concentrated into mass concentration is 2g/mL；
4) by above-mentioned steps 1)~3) extract volatile oil and extracting solution mixing, by above-mentioned addition auxiliary agent, be made different doses
The compound plant preparation of type.
Above-mentioned steps 2 of the present invention) in organic solvent can be organic solvent commonly used in the art, including but not limited to second
Alcohol, methanol or acetone soln or their mixed solution；
As one of embodiment, the organic solvent is preferably 60~80% ethanol solution.
Preparation the present invention also provides the composition for being used for the ginseng prevention and control of plant diseases, pest control described in one kind and containing the composition
Using the application includes but is not limited to the prevention and control of plant diseases, pest control in Araliaceae planting process；
As one of embodiment of the present invention, the insect pest during preferably ginseng, American Ginseng or notoginseng planting is applied
Prevention and treatment.
The present invention is right using different extracting methods according to the characteristics of various natural plant active components and the mechanism of action
Various plants extract rationally assembles, in addition to containing plant volatile oil, alkaloid, also containing a large amount of other biological active matters
Matter gives full play to the synergistic function between various active constituents, the invention overcome most plants source pesticide sterilization at
Divide single, the simple limitation of the mechanism of action, improve the integrated control effect of product, works rapidly, the lasting period is long, avoids
The generation of pest resistance to insecticide.
The advantage of the present composition and the preparation containing the composition is not only prevent the hair of ginseng pest and disease damage
It is raw, it can also effectively treat, and harmful substance will not be left in the soil.The yield for not only increasing ginseng, is also improved
Ginseng quality.There is residual to overcome existing fungicide, the skill seriously endangered is caused to health and ecological environment
Art defect, while the yield and quality of ginseng is improved, there is great economic benefit.
Specific embodiment
Following embodiment does not limit effective range of the invention for the present invention is further explained in any manner.
Embodiment 1 (emulsion):
Asarum 5kg, rhizoma acori graminei 5kg are weighed, 200 liters of water are added in co-grinding, and refluxing extraction volatile oil obtains volatile oil
30g。
Polygonum cuspidate 1kg, Tarragon 6kg, Herba Andrographitis 2kg and Chinese ephedra 0.5kg are weighed, 190 liter of 60% ethyl alcohol is added, it is small to impregnate 48
When, it is during which primary every stirring in 3 hours, it extracts 3 times, combined extract, filtering obtains filtrate and residue.Filtrate depressurize dense
Contracting, bath temperature are 80 DEG C.It is concentrated into the 1/2 of primary dose, that is, is settled to 4.75kg.
Chinese bulbul 5kg, kuh-seng 5kg and dandelion 3kg are weighed, 260 liters of water are added, refluxing extraction 8 hours, extracts 3 times, closes
And extracting solution, filtering, 80 DEG C are concentrated under reduced pressure into the 1/2 of primary dose, that is, are settled to 6.5kg.
Above-mentioned volatile oil and extracting solution are merged and mixed, extract 11.28kg is obtained, emulsifier agriculture breast 300,2kg is added,
High osmotic agent azone 2kg, sodium citrate 0.3kg and water 150kg be added blender it is pre-dispersed (3000 revs/min of the speed of stirring, when
Between 60 minutes) and high shear (3000 revs/min of shear velocity, 60 minutes time) obtain Complex Emulsion.
Embodiment 2 (missible oil):
Asarum 1kg, rhizoma acori graminei 1kg are weighed, 20 liters of water are added in co-grinding, and refluxing extraction volatile oil obtains volatile oil 4g.
Polygonum cuspidate 0.1kg, Tarragon 1kg, Herba Andrographitis 0.5kg and Chinese ephedra 0.1kg are weighed, 20 liter of 60% ethyl alcohol is added, impregnates 24,
Period is primary every stirring in 3 hours, extracts 2 times, combined extract, and filtering obtains filtrate and residue.Filtrate is concentrated under reduced pressure,
Bath temperature is 60 DEG C.It is concentrated into the 1/2 of primary dose, that is, is settled to 0.85kg.
Chinese bulbul 1kg, kuh-seng 1kg and dandelion 0.5kg are weighed, 25 liters of water are added, refluxing extraction 4 hours, extract twice,
Combined extract, filtering, 50 DEG C are concentrated under reduced pressure into the 1/2 of primary dose, that is, are settled to 1.25kg.
Above-mentioned volatile oil and extracting solution are merged and mixed, extract 2.104kg is obtained, emulsifier NP-210.4kg is added,
High osmotic agent azone 0.4kg, sodium citrate 0.06kg and cosolvent ethyl alcohol 6kg, which are stirred evenly, is made oily phase, and blender is added and divides in advance
Dissipate (3000 revs/min of the speed of stirring, 60 minutes time) and high shear (3000 revs/min of shear velocity, 60 minutes time)
Obtain compound missible oil.
Embodiment 3 (pulvis):
Asarum 3kg, rhizoma acori graminei 3kg are weighed, 90 liters of water are added in co-grinding, and refluxing extraction volatile oil obtains volatile oil 14g.
Polygonum cuspidate 0.5kg, Tarragon 4kg, Herba Andrographitis 1.3kg and Chinese ephedra 0.3kg are weighed, 90 liter of 60% ethyl alcohol is added, impregnates 36
Hour, it is during which primary every stirring in 3 hours, it extracts 3 times, combined extract, filtering obtains filtrate and residue.Filtrate is depressurized
Concentration, bath temperature are 70 DEG C.It is concentrated into the 1/2 of primary dose, that is, is settled to 3.05kg.
Chinese bulbul 3kg, kuh-seng 3kg and dandelion 1.5kg are weighed, 120 liters of water are added, refluxing extraction 6 hours, extract 3 times,
Combined extract, filtering, 70 DEG C are concentrated under reduced pressure into the 1/2 of primary dose, that is, are settled to 3.75kg.
Above-mentioned volatile oil and extracting solution are merged and mixed, extract 6.814kg is obtained, the peaceful cream 32 of emulsifier is added
Pre-dispersed (the speed 3000 of stirring of blender is added in 1.5kg, high osmotic agent azone 1.3kg, sodium citrate 0.2kg and water 20kg
Rev/min, 60 minutes time) and high shear (3000 revs/min of shear velocity, 60 minutes time), further decompression drying, powder
Broken 1000 mesh of mistake, obtains compounding powder.
1 indoor application of experimental example inhibits Ginseng Rhizoctonia Solani, black spot, rust rot, epidemic disease pathogen effect experiment.
Drug and reagent source: the emulsion of the preparation of embodiment 1, sharp gram of Ai Er；
Reagent source: 80% ethyl alcohol.
Indoor antibacterial test measurement
Drug effect is measured using growth rate method.PDA (potato dextrose agar) culture medium processed under aseptic technique
Plate accesses after to be condensed for trying strain.When bacterium piece is uniformly covered with culture dish, bacteria cake is cut.Aseptically, by plant
Culture medium is added according to 1: 9 ratio in preparation and PDA culture medium, be made concentration 1.0mg/mL, 2.0mg/mL, 3.0mg/mL and
The band medicine culture medium of 5.0mg/mL, repeats 3~4 times.80% ethyl alcohol of the dosage+PDA culture mediums such as setting are blank control, with love
Sharp gram of that makees medicament control.
Bacteria cake moves into band medicine culture medium, colony diameter length to be compareed to more than plate diameter 2/3, without covering with plate
When, the colony diameter for examination pathogen on different band poison culture medium is measured using crossing method with ruler, 3 repetitions are asked
Its average value.
It calculates growth inhibition ratio (%), calculates virulence regression equation and EC50 with Finney probit value method.
The antibacterial virulence test result of 1. galenical of table
| Pathogen | Virulence equation | EC50(g/l) | Correlation coefficient r |
| Ginseng Blight In China | Y=3.1722+1.1703 | 7.89 | 0.9536 |
| Ginseng Rhizoctonia Solani | Y=8.1133x+3.4366 | 9.81 | 0.9681 |
| Ginseng rust rot | Y=7.5351x+2.2709 | 6.51 | 0.9533 |
| Black fleck disease of ginseng | Y=5.1786+2.0922 | 6.03 | 0.9617 |
According to 36 hours measurement results of bacterium are connect, galenical is most strong to the toxicity of black fleck disease of ginseng, EC50 6.03g/L,
It is most weak to the toxicity of Ginseng Rhizoctonia Solani, EC50 9.81g/L.There is stronger inhibition to the above-mentioned ginseng disease fungus for examination
Effect, same pathogen are also variant to the sensibility of galenical.
2 Field information of experimental example inhibit Ginseng Rhizoctonia Solani, black spot, rust rot, epidemic disease pathogen effect experiment
Medicine source: the emulsion of the preparation of embodiment 1, sharp gram of Ai Er, 50% carbendazim, native bacterium kill；
Reagent source: 80% ethyl alcohol.
Experiment place is located at Jilin Province's Fusong County.Topography is flat, soil property is consistent, the place of ginseng growing way equilibrium.Using with
Machine block design, in test, only one check plot, check plot and processing group are arranged at random together in each duplicate block
Column, respectively repeat in treating number it is identical.Record change of illness state index.Experimental result is shown in Table 2.
2 field efficacy measurement result of table
Emulsion is not compared with the control, significant to ginseng disease protective effect as can be drawn from Table 3, but has and significantly control
Treatment effect, with compare between there are extremely significant differences, had differences between emulsion and comparison medicament in 0.05 level of signifiance, and
There is extremely significant difference between 0.01 horizontal upper emulsion and comparison medicament, illustrates that emulsion has apparent treatment to make ginseng pathogen
With.
Claims (13)
1. a kind of composition of the prevention and treatment for Ginseng Blight In China, damping-off, rust rot or black spot, which is characterized in that described group
It is composed of the following components in parts by weight to close object:
0.1~1.0 part of polygonum cuspidate, 1~5 part of asarum, 1~5 part of the Chinese bulbul, 1~5 part of kuh-seng, 1~6 part of Artemisia capillaris, Herba Andrographitis 0.5
~2 parts, 1~5 part of rhizoma acori graminei, 0.1~0.5 part and 0.5~3 part of dandelion of Chinese ephedra.
2. composition according to claim 1, which is characterized in that the composition is in parts by weight by following components group
At: 0.5~0.8 part of polygonum cuspidate, 2~4 parts of asarum, 2~4 parts of the Chinese bulbul, 2~4 parts of kuh-seng, 2~5 parts of Tarragon, Herba Andrographitis 0.8~
1.8 parts, 2~4 parts of rhizoma acori graminei, 0.2~0.4 part of Chinese ephedra and 0.8~2 part of dandelion.
3. composition according to claim 1 or 2, which is characterized in that the composition is in parts by weight by following components
Composition: 0.7 part of polygonum cuspidate, 3 parts of asarum, 3 parts of the Chinese bulbul, 3 parts of kuh-seng, 4 parts of Tarragon, 1 part of Herba Andrographitis, 3 parts of rhizoma acori graminei, Chinese ephedra
0.3 part and 1.5 parts of dandelion；
1 part of polygonum cuspidate, 5 parts of asarum, 5 parts of the Chinese bulbul, 5 parts of kuh-seng, 6 parts of Tarragon, 2 parts of Herba Andrographitis, 5 parts of rhizoma acori graminei, 0.5 part of Chinese ephedra
With 3 parts of dandelion；
0.1 part of polygonum cuspidate, 1 part of asarum, 1 part of the Chinese bulbul, 1 part of kuh-seng, 1 part of Tarragon, 0.5 part of Herba Andrographitis, 1 part of rhizoma acori graminei, Chinese ephedra
0.1 part and 0.5 part of dandelion；Or
0.5 part of polygonum cuspidate, 3 parts of asarum, 3 parts of the Chinese bulbul, 3 parts of kuh-seng, 4 parts of Tarragon, 1.3 parts of Herba Andrographitis, 3 parts of rhizoma acori graminei, Chinese ephedra
0.3 part and 1.5 parts of dandelion.
4. the preparation containing claim 1,2 or 3 compositions, which is characterized in that the preparation also contains in parts by weight 1
~40 parts of auxiliary agent, the auxiliary agent are selected from emulsifier, cosolvent or high osmotic agent or two or more combination in them.
5. preparation according to claim 4, which is characterized in that the emulsifier is selected from agriculture breast 300, NP-21 or peaceful cream 32
Number, the high osmotic agent is azone, and institute's cosolvent is ethyl alcohol.
6. preparation according to claim 5, which is characterized in that the preparation further comprises that pH value is 2~9；The pH
Value regulator is sodium citrate.
7. preparation according to claim 6, which is characterized in that the pH value is 3~7.
8. according to any preparation of claim 4~7, which is characterized in that the preparation is emulsion, missible oil or pulvis.
9. a kind of preparation method of any preparation of claim 4~8, which is characterized in that the method includes the following steps:
1) asarum and rhizoma acori graminei are weighed, using extraction by steam distillation volatile oil；
2) it weighs polygonum cuspidate, Tarragon, Herba Andrographitis and Chinese ephedra, the organic solvents of 10~20 times of amounts is added, impregnate 24~48h, with shaking
Bed interval concussion 12h, is extracted 2~3 times, decompression filters, and obtains filtrate and residue, filtrate is concentrated under reduced pressure, bath temperature 50
~80 DEG C, being concentrated into mass concentration is 2g/mL；
3) Chinese bulbul, kuh-seng and dandelion are weighed, 10~20 times of water is added, refluxing extraction 4~8 hours, extracts 2~3 times, mistake
Filter, filtrate are concentrated under reduced pressure, and bath temperature is 50~80 DEG C, and being concentrated into mass concentration is 2g/mL；
4) by above-mentioned steps 1)~3) extract volatile oil and extracting solution mixing, auxiliary agent is then added, corresponding dosage form is made.
10. according to the method described in claim 9, it is characterized in that, the organic solvent be ethyl alcohol, methanol or acetone soln,
Or their mixed solution.
11. according to the method described in claim 10, it is characterized in that, the ethanol solution that the organic solvent is 60~80%.
12. any composition of claim 1-3 or any preparation of claim 4~8 or claim 9~11 are any
The application of preparation prepared by method, which is characterized in that the application is epidemic disease, the damping-off, rust rot or black of Araliaceae
The prevention and treatment of pinta.
13. application according to claim 12, which is characterized in that the Araliaceae is ginseng, American Ginseng or Radix Notoginseng.
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| CN102784350A (en) * | 2012-08-07 | 2012-11-21 | 孙雅儒 | Mite killing bactericide and preparation method of mite killing bactericide |
| CN102812971A (en) * | 2012-08-08 | 2012-12-12 | 山东省农业科学院植物保护研究所 | Compound traditional Chinese medicine extract for killing plant pathogenic bacteria and insect pests |
| CN103404543A (en) * | 2013-06-28 | 2013-11-27 | 句容市后白镇玉琴果品专业合作社 | Pear tree insect repellent agent and preparation and application methods of pear tree insect repellent agent |
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| CN102784350A (en) * | 2012-08-07 | 2012-11-21 | 孙雅儒 | Mite killing bactericide and preparation method of mite killing bactericide |
| CN102812971A (en) * | 2012-08-08 | 2012-12-12 | 山东省农业科学院植物保护研究所 | Compound traditional Chinese medicine extract for killing plant pathogenic bacteria and insect pests |
| CN103404543A (en) * | 2013-06-28 | 2013-11-27 | 句容市后白镇玉琴果品专业合作社 | Pear tree insect repellent agent and preparation and application methods of pear tree insect repellent agent |
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