CN105961010A - Method for cultivating ludisia discolor by means of orchid mycorrhizae - Google Patents
Method for cultivating ludisia discolor by means of orchid mycorrhizae Download PDFInfo
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- CN105961010A CN105961010A CN201610400150.0A CN201610400150A CN105961010A CN 105961010 A CN105961010 A CN 105961010A CN 201610400150 A CN201610400150 A CN 201610400150A CN 105961010 A CN105961010 A CN 105961010A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Abstract
The invention provides a method for cultivating ludisia discolor by means of orchid mycorrhizae. Orchid mycorrhizae is cleaned with tap water and subjected to surface sterilization with ethyl alcohol of 75%, nodular tissue is cut off on an ultra-clean work table and placed in a fungus plate medium till hyphae grow, and hyphae are extracted and placed in the same plate medium for purification cultivation; cultivation is conducted at 25-30 DEG C for 7-8 days till the plat medium is covered with hyphae, and hypha blocks on a plate are cut and placed in a mixed cultivation fungus sack for inoculation; cultivation is conducted at 24-28 DEG C for 35-40 days till hyphae grow out of the fungus sack; the fungus sack is mixed with a matrix where orchid was planted to serve as a ludisia discolor cultivation medium; then the medium is inoculated with ludisia discolor tissue cultivation seedlings for cultivation. Wild ludisia discolor tissue cultivation seedlings are adopted, the matrix where orchid was planted is mixed with the endophytic fungus mixed medium cultivated artificially to cultivate ludisia discolor, plant height and weight are increased remarkably, product quality is improved remarkably, and then the medical value of ludisia discolor can be better developed and utilized.
Description
Technical field
The invention belongs to field of plant cultivation, be specifically related to a kind of method utilizing orchid root bacteria cultivation hectolitre pine.
Background technology
Hectolitre pine: orchid blood aspidistra Ludisia
Discolor (Ker-Gawl.) A. Rich., is the south of Fujian Province medical herbs simply having much local characteristic among the people.There is the effects such as clearing away heat and cooling blood, body-fluid-generating heat-clearing, diuretic dredging collateral, cough-relieving, can be used for treating high fever, laryngopharynx swelling and pain, nameless gall, the spleen-stomach disease and inflammation etc.." China book on Chinese herbal medicine " records its effect: " lung moistening, spleen invigorating, calm the nerves, cure mainly pulmonary tuberculosis hemoptysis, neurasthenia, inappetence ".Growing environment is required harsher by hectolitre pine, is usually grown in dark and damp rock seam, mostly is more rich endogenetic fungus vegetatively.Its growth course needs to form symbiosis with fungus.
nullThe hectolitre fresh and alive plant of pine is manually planted experimentally the investigation of cultivation by artificial culture situation according to part distributor，On business growing environment is required harsher by Lycopodium clavatum，More authoritative mature technology is not had to be referred to，Few people carry out cultivation and plant experimentally research artificial planting technique. and the hectolitre pine that market is sold is generally by fresh and alive plant of stocking up，Train with sand、Water planting、Three kinds of forms of earth culture carry out short-term cultivation. and husky training is with its good permeability、Mineral nutrition is abundant、Hydrops less will not the advantage such as rotten and be that majority use，But common technique content is low，Biological yield increases inconspicuous. and husky training is not for scale breeding，And it is primarily used to proper extension Fresh Plants life cycle，The supply of material of fresh and alive plant is arranged according to sales volume，To guarantee to pay no attention to cut-off goods for a long time. in production practices，There is presently no and it is used cuttage、The vegetative propagation techniques such as tissue culture carry out the precedent of scale breeding.
Owing to hectolitre pine natural propagation power is poor, we use tissue culture technology fast breeding Seedling, because tissue cultured seedling is under aseptic condition growth, self root can not be utilized well to absorb soil Middle nutrition material when implanting and cultivating, poor growth, utilization was planted Cymbidium ensifolium (L.) Sw. base material (its base material is relatively suitable for endogenetic fungus breeding) and was added the endogenetic fungus mixed-matrix cultivation hectolitre pine of artificial culture, fungus can form the mycorhiza system of a symbiosis after invading hectolitre Radix Pini massonianae portion, growth promoter for hectolitre pine provides the nutrient substance being correlated with, the present invention crosses the Seedling of tissue culture with wild hectolitre loose warp, use the endogenetic fungus mixed-matrix cultivation hectolitre pine planting Cymbidium ensifolium (L.) Sw. base material addition artificial culture, can significantly promote that plant increases weightening finish and improves product quality, thus preferably develop the medical value of hectolitre pine.
Summary of the invention
It is an object of the invention to provide a kind of method utilizing orchid root bacteria cultivation hectolitre pine, the present invention crosses the Seedling of tissue culture with wild hectolitre loose warp, use the endogenetic fungus mixed-matrix cultivation hectolitre pine planting Cymbidium ensifolium (L.) Sw. base material addition artificial culture, can significantly promote that plant increases weightening finish and improves product quality, thus preferably develop the medical value of hectolitre pine.
For achieving the above object, the present invention adopts the following technical scheme that
The method utilizing orchid root bacteria cultivation hectolitre pine, comprises the steps:
(1) fungal component separation and Culture；Cymbidium ensifolium (L.) Sw. mycorhiza tap water cleans up, and carries out surface sterilization with the ethanol that volume fraction is 75%, cuts warty tissue on superclean bench, moves into fungus plating medium and treats that mycelia grows, and extracts mycelia immigration same plane culture medium and carries out purifying cultivation；Cultivating 7-8 days mycelia at 25-30 DEG C and cover with plating medium, on cutting flat board, inoculated by hypha block is to multi strain co cultivation bacterium bag；Cultivate under the conditions of 24-28 DEG C and within 35-40 days, treat mycelia length saturating bacterium bag；Saturating for mycelia length bacterium bag is mixed with the base material planting Cymbidium ensifolium (L.) Sw., as hectolitre pine cultivation matrix；
(2) hectolitre pine growing and cultivation；When hectolitre pine tissue culturing seedling head takes out from culture bottle from 3-3.5cm, with tap water, root culture medium is rinsed well；On hectolitre pine cultivation matrix, spread one layer of 1cm yellow soil, clean hectolitre pine tissue culturing seedling is planted in substrate, waters the permeable place being placed on and not having direct sunlight, keep 85% humidity；Through cultivation in 50-60 days, root was clearly visible root tuber mycorrhiza.
Described multi strain co cultivation bacterium bag composition is: wood flour 87-88%, Semen Maydis powder 2-3%, pulverized limestone 0.5-1%, Testa Tritici bran 7-8%, sucrose 0.5-1%.The part by weight that in step (1), mycelia length saturating bacterium bag mixes with the base material planting Cymbidium ensifolium (L.) Sw. is 1:30.
Described fungus plating medium composition is: peptone 5.0g/L, Carnis Bovis seu Bubali cream 3.0g/L, agar 20.0g/L.
It is an advantage of the current invention that:
The present invention crosses the Seedling of tissue culture with wild hectolitre loose warp, employing was planted Cymbidium ensifolium (L.) Sw. base material (current implant mass oncidiumLuridum mainly uses small stone and bark mixed-matrix (Fig. 5), and planting process needs to regularly replace base material) and was added the endogenetic fungus mixed-matrix cultivation hectolitre pine of artificial culture.So present invention can make full use of refuse plantation hectolitre pine, is substantially reduced planting cost.The most numerous and scale cultivates hectolitre pine, thus preferably develops the medical value of hectolitre pine.
This invention achieves the quick reproduction technique under aseptic condition and Efficient Cultivation, survival rate to more than 90%, plants 120 days Weight per plant and improves about 30% than generic media.Overcome the easy bad root phenomenon of generic media, thus solve the problem that the poor nature wild resource caused of hectolitre pine natural propagation power is deficient.Allow this Chinese herbal medicine having long applicating history, drug effect definite preferably develop, fill up the technical research of vegetative propagation technique and Efficient Cultivation.A new mode is opened up for hectolitre pine artificial culture.
Accompanying drawing explanation
Fig. 1 Endophytic Fungal Hyphae.
The visible endogenetic fungus of Fig. 2 hectolitre Tricholoma matsutake (lto et lmai) Singer root section.
Fig. 3 hectolitre Tricholoma matsutake (lto et lmai) Singer root.
The hectolitre pine of Fig. 4 mixed resin cultivation.
Fig. 5 small stone and the oncidiumLuridum of bark mixed-matrix plantation.
One layer of yellow soil lower floor mixed-matrix is spread above Fig. 6.
Detailed description of the invention
Embodiment 1
The method utilizing orchid root bacteria cultivation hectolitre pine, comprises the steps:
(1) fungal component separation and Culture；Cymbidium ensifolium (L.) Sw. mycorhiza tap water cleans up, and carries out surface sterilization with the ethanol that volume fraction is 75%, cuts warty tissue on superclean bench, moves into fungus plating medium and treats that mycelia grows, and extracts mycelia immigration same plane culture medium and carries out purifying cultivation；Cultivating 7 days mycelia at 25 DEG C and cover with plating medium, on cutting flat board, inoculated by hypha block is to multi strain co cultivation bacterium bag；Cultivate under the conditions of 24 DEG C and within 35 days, treat mycelia length saturating bacterium bag；Saturating for mycelia length bacterium bag is mixed (1:30) with the base material planting Cymbidium ensifolium (L.) Sw., as hectolitre pine cultivation matrix；
(2) hectolitre pine growing and cultivation；When hectolitre pine tissue culturing seedling head takes out from culture bottle from 3cm, with tap water, root culture medium is rinsed well；On hectolitre pine cultivation matrix, spread one layer of 1cm yellow soil, clean hectolitre pine tissue culturing seedling is planted in substrate, waters the permeable place being placed on and not having direct sunlight, keep 85% humidity；Through cultivation in 50 days, root was clearly visible root tuber mycorrhiza.
Described multi strain co cultivation bacterium bag composition is: wood flour 87%, Semen Maydis powder 3%, pulverized limestone 1%, Testa Tritici bran 8%, sucrose 1%.The part by weight that in step (1), mycelia length saturating bacterium bag mixes with the base material planting Cymbidium ensifolium (L.) Sw. is 1:30.
Described fungus plating medium composition is: peptone 5.0g/L, Carnis Bovis seu Bubali cream 3.0g/L, agar 20.0g/L.
This invention achieves the quick reproduction technique under aseptic condition and Efficient Cultivation, survival rate to 96%, plants 120 days Weight per plant and improves 35% than generic media.
Embodiment 2
The method utilizing orchid root bacteria cultivation hectolitre pine, comprises the steps:
(1) fungal component separation and Culture；Cymbidium ensifolium (L.) Sw. mycorhiza tap water cleans up, and carries out surface sterilization with the ethanol that volume fraction is 75%, cuts warty tissue on superclean bench, moves into fungus plating medium and treats that mycelia grows, and extracts mycelia immigration same plane culture medium and carries out purifying cultivation；Cultivating 8 days mycelia at 30 DEG C and cover with plating medium, on cutting flat board, inoculated by hypha block is to multi strain co cultivation bacterium bag；Cultivate under the conditions of 28 DEG C and within 40 days, treat mycelia length saturating bacterium bag；Saturating for mycelia length bacterium bag is mixed (1:30) with the base material planting Cymbidium ensifolium (L.) Sw., as hectolitre pine cultivation matrix；
(2) hectolitre pine growing and cultivation；When hectolitre pine tissue culturing seedling head takes out from culture bottle from 3.5cm, with tap water, root culture medium is rinsed well；On hectolitre pine cultivation matrix, spread one layer of 1cm yellow soil, clean hectolitre pine tissue culturing seedling is planted in substrate, waters the permeable place being placed on and not having direct sunlight, keep 85% humidity；Through cultivation in 60 days, root was clearly visible root tuber mycorrhiza.
Described multi strain co cultivation bacterium bag composition is: wood flour 88%, Semen Maydis powder 2%, pulverized limestone 0.5%, Testa Tritici bran 7%, sucrose 0.5%.The part by weight that in step (1), mycelia length saturating bacterium bag mixes with the base material planting Cymbidium ensifolium (L.) Sw. is 1:30.
Described fungus plating medium composition is: peptone 5.0g/L, Carnis Bovis seu Bubali cream 3.0g/L, agar 20.0g/L.
This invention achieves the quick reproduction technique under aseptic condition and Efficient Cultivation, survival rate to 96%, plants 120 days Weight per plant and improves 35% than generic media.
Embodiment 3
The method utilizing orchid root bacteria cultivation hectolitre pine, comprises the steps:
(1) fungal component separation and Culture；Cymbidium ensifolium (L.) Sw. mycorhiza tap water cleans up, and carries out surface sterilization with the ethanol that volume fraction is 75%, cuts warty tissue on superclean bench, moves into fungus plating medium and treats that mycelia grows, and extracts mycelia immigration same plane culture medium and carries out purifying cultivation；Cultivating 8 days mycelia at 28 DEG C and cover with plating medium, on cutting flat board, inoculated by hypha block is to multi strain co cultivation bacterium bag；Cultivate under the conditions of 25 DEG C and within 38 days, treat mycelia length saturating bacterium bag；Saturating for mycelia length bacterium bag is mixed (1:30) with the base material planting Cymbidium ensifolium (L.) Sw., as hectolitre pine cultivation matrix；
(2) hectolitre pine growing and cultivation；When hectolitre pine tissue culturing seedling head takes out from culture bottle from 3.5cm, with tap water, root culture medium is rinsed well；On hectolitre pine cultivation matrix, spread one layer of 1cm yellow soil, clean hectolitre pine tissue culturing seedling is planted in substrate, waters the permeable place being placed on and not having direct sunlight, keep 85% humidity；Through cultivation in 55 days, root was clearly visible root tuber mycorrhiza.
Described multi strain co cultivation bacterium bag composition is: wood flour 87%, Semen Maydis powder 3%, pulverized limestone 1%, Testa Tritici bran 8%, sucrose 1%.The part by weight that in step (1), mycelia length saturating bacterium bag mixes with the base material planting Cymbidium ensifolium (L.) Sw. is 1:30.
Described fungus plating medium composition is: peptone 5.0g/L, Carnis Bovis seu Bubali cream 3.0g/L, agar 20.0g/L.
This invention achieves the quick reproduction technique under aseptic condition and Efficient Cultivation, survival rate to 96%, plants 120 days Weight per plant and improves 35% than generic media.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent and modification, all should belong to the covering scope of the present invention.
Claims (4)
1. the method utilizing orchid root bacteria cultivation hectolitre pine, it is characterised in that: described method comprises the steps:
(1) fungal component separation and Culture；Cymbidium ensifolium (L.) Sw. mycorhiza tap water cleans up, and carries out surface sterilization with the ethanol that volume fraction is 75%, cuts warty tissue on superclean bench, moves into fungus plating medium and treats that mycelia grows, and extracts mycelia immigration same plane culture medium and carries out purifying cultivation；Cultivating 7-8 days mycelia at 25-30 DEG C and cover with plating medium, on cutting flat board, inoculated by hypha block is to multi strain co cultivation bacterium bag；Cultivate under the conditions of 24-28 DEG C and within 35-40 days, treat mycelia length saturating bacterium bag；Saturating for mycelia length bacterium bag is mixed with the base material planting Cymbidium ensifolium (L.) Sw., as hectolitre pine cultivation matrix；
(2) hectolitre pine growing and cultivation；When hectolitre pine tissue culturing seedling head takes out from culture bottle from 3-3.5cm, with tap water, root culture medium is rinsed well；On hectolitre pine cultivation matrix, spread one layer of 1cm yellow soil, clean hectolitre pine tissue culturing seedling is planted in substrate, waters the permeable place being placed on and not having direct sunlight, keep 85% humidity；Through cultivation in 50-60 days, root was clearly visible root tuber mycorrhiza.
The method utilizing orchid root bacteria cultivation hectolitre pine the most according to claim 1, it is characterised in that: described multi strain co cultivation bacterium bag composition is: wood flour 87-88%, Semen Maydis powder 2-3%, pulverized limestone 0.5-1%, Testa Tritici bran 7-8%, sucrose 0.5-1%.
The method utilizing orchid root bacteria cultivation hectolitre pine the most according to claim 1, it is characterised in that: the part by weight that in step (1), mycelia length saturating bacterium bag mixes with the base material planting Cymbidium ensifolium (L.) Sw. is 1:30.
The method utilizing orchid root bacteria cultivation hectolitre pine the most according to claim 1, it is characterised in that: described fungus plating medium composition is: peptone 5.0g/L, Carnis Bovis seu Bubali cream 3.0g/L, agar 20.0g/L.
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Cited By (4)
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| CN108174778A (en) * | 2018-02-26 | 2018-06-19 | 福建农林大学 | A kind of culture apparatus and its method of hectolitre pine |
| CN108271652A (en) * | 2017-12-14 | 2018-07-13 | 福建省热带作物科学研究所 | A kind of hectolitre turpentine cultural method |
| CN111543278A (en) * | 2020-05-22 | 2020-08-18 | 阚尔谆 | Breeding mode of orchid seeds |
| CN116622553A (en) * | 2023-04-07 | 2023-08-22 | 四川省食用菌研究所 | Lactarius deliciosus mycorrhiza growth promoting bacterium, and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116622553A (en) * | 2023-04-07 | 2023-08-22 | 四川省食用菌研究所 | Lactarius deliciosus mycorrhiza growth promoting bacterium, and preparation method and application thereof |
| CN116622553B (en) * | 2023-04-07 | 2023-12-08 | 四川省食用菌研究所 | Lactarius deliciosus mycorrhiza growth promoting bacterium, and preparation method and application thereof |
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