CN105960446B - Microorganism compositionss - Google Patents
Microorganism compositionss Download PDFInfo
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- CN105960446B CN105960446B CN201580003186.9A CN201580003186A CN105960446B CN 105960446 B CN105960446 B CN 105960446B CN 201580003186 A CN201580003186 A CN 201580003186A CN 105960446 B CN105960446 B CN 105960446B
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- microorganism
- glycerol
- compositionss
- sorbitol
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- 244000005700 microbiome Species 0.000 title claims abstract description 52
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 204
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 64
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims abstract description 62
- 239000000600 sorbitol Substances 0.000 claims abstract description 62
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims abstract description 58
- 239000002689 soil Substances 0.000 claims abstract description 44
- 239000002736 nonionic surfactant Substances 0.000 claims abstract description 38
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims abstract description 27
- 229930195725 Mannitol Natural products 0.000 claims abstract description 24
- 239000000594 mannitol Substances 0.000 claims abstract description 24
- 235000010355 mannitol Nutrition 0.000 claims abstract description 24
- 150000005846 sugar alcohols Chemical class 0.000 claims abstract description 19
- 238000000746 purification Methods 0.000 claims abstract description 9
- -1 poly- Oxygen ethylene lauryl ether Chemical compound 0.000 claims description 24
- 150000001875 compounds Chemical class 0.000 claims description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims 1
- 235000010356 sorbitol Nutrition 0.000 abstract description 61
- 238000002425 crystallisation Methods 0.000 abstract description 32
- 230000008025 crystallization Effects 0.000 abstract description 32
- 150000004045 organic chlorine compounds Chemical class 0.000 abstract description 6
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 abstract description 2
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 abstract description 2
- 239000000811 xylitol Substances 0.000 abstract description 2
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 abstract description 2
- 235000010447 xylitol Nutrition 0.000 abstract description 2
- 229960002675 xylitol Drugs 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 description 27
- 239000003673 groundwater Substances 0.000 description 22
- 239000000203 mixture Substances 0.000 description 19
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 12
- 238000000034 method Methods 0.000 description 11
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 10
- 238000012360 testing method Methods 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 238000002347 injection Methods 0.000 description 8
- 239000007924 injection Substances 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 7
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 239000013078 crystal Substances 0.000 description 6
- 238000000354 decomposition reaction Methods 0.000 description 6
- 230000008859 change Effects 0.000 description 5
- 239000001257 hydrogen Substances 0.000 description 5
- 229910052739 hydrogen Inorganic materials 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000004094 surface-active agent Substances 0.000 description 5
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 4
- CYTYCFOTNPOANT-UHFFFAOYSA-N Perchloroethylene Chemical group ClC(Cl)=C(Cl)Cl CYTYCFOTNPOANT-UHFFFAOYSA-N 0.000 description 4
- 238000009835 boiling Methods 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 229920000259 polyoxyethylene lauryl ether Polymers 0.000 description 4
- 229950011008 tetrachloroethylene Drugs 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 3
- 230000003213 activating effect Effects 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 235000014113 dietary fatty acids Nutrition 0.000 description 3
- 239000000194 fatty acid Substances 0.000 description 3
- 229930195729 fatty acid Natural products 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000006174 pH buffer Substances 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 229920001214 Polysorbate 60 Polymers 0.000 description 2
- 241000220324 Pyrus Species 0.000 description 2
- LWZFANDGMFTDAV-BURFUSLBSA-N [(2r)-2-[(2r,3r,4s)-3,4-dihydroxyoxolan-2-yl]-2-hydroxyethyl] dodecanoate Chemical compound CCCCCCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O LWZFANDGMFTDAV-BURFUSLBSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000852 hydrogen donor Substances 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 235000021017 pears Nutrition 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000006722 reduction reaction Methods 0.000 description 2
- 230000035939 shock Effects 0.000 description 2
- 235000011067 sorbitan monolaureate Nutrition 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 125000005480 straight-chain fatty acid group Chemical group 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical compound OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 description 1
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
- FFJCNSLCJOQHKM-CLFAGFIQSA-N (z)-1-[(z)-octadec-9-enoxy]octadec-9-ene Chemical compound CCCCCCCC\C=C/CCCCCCCCOCCCCCCCC\C=C/CCCCCCCC FFJCNSLCJOQHKM-CLFAGFIQSA-N 0.000 description 1
- SCYULBFZEHDVBN-UHFFFAOYSA-N 1,1-Dichloroethane Chemical class CC(Cl)Cl SCYULBFZEHDVBN-UHFFFAOYSA-N 0.000 description 1
- ZORQXIQZAOLNGE-UHFFFAOYSA-N 1,1-difluorocyclohexane Chemical compound FC1(F)CCCCC1 ZORQXIQZAOLNGE-UHFFFAOYSA-N 0.000 description 1
- XDOFQFKRPWOURC-UHFFFAOYSA-N 16-methylheptadecanoic acid Chemical compound CC(C)CCCCCCCCCCCCCCC(O)=O XDOFQFKRPWOURC-UHFFFAOYSA-N 0.000 description 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 244000045232 Canavalia ensiformis Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 235000013162 Cocos nucifera Nutrition 0.000 description 1
- 244000060011 Cocos nucifera Species 0.000 description 1
- 241000605909 Fusobacterium Species 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
- 235000010617 Phaseolus lunatus Nutrition 0.000 description 1
- 244000173166 Pyrus ussuriensis Species 0.000 description 1
- 235000011572 Pyrus ussuriensis Nutrition 0.000 description 1
- IYFATESGLOUGBX-YVNJGZBMSA-N Sorbitan monopalmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O IYFATESGLOUGBX-YVNJGZBMSA-N 0.000 description 1
- HVUMOYIDDBPOLL-XWVZOOPGSA-N Sorbitan monostearate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O HVUMOYIDDBPOLL-XWVZOOPGSA-N 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- XSTXAVWGXDQKEL-UHFFFAOYSA-N Trichloroethylene Chemical group ClC=C(Cl)Cl XSTXAVWGXDQKEL-UHFFFAOYSA-N 0.000 description 1
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical class ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- 150000005215 alkyl ethers Chemical class 0.000 description 1
- 150000008064 anhydrides Chemical class 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000006298 dechlorination reaction Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- USIUVYZYUHIAEV-UHFFFAOYSA-N diphenyl ether Chemical class C=1C=CC=CC=1OC1=CC=CC=C1 USIUVYZYUHIAEV-UHFFFAOYSA-N 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical class CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920005862 polyol Polymers 0.000 description 1
- 150000003077 polyols Chemical class 0.000 description 1
- 239000000249 polyoxyethylene sorbitan monopalmitate Substances 0.000 description 1
- 235000010483 polyoxyethylene sorbitan monopalmitate Nutrition 0.000 description 1
- 239000001818 polyoxyethylene sorbitan monostearate Substances 0.000 description 1
- 235000010989 polyoxyethylene sorbitan monostearate Nutrition 0.000 description 1
- 239000001816 polyoxyethylene sorbitan tristearate Substances 0.000 description 1
- 235000010988 polyoxyethylene sorbitan tristearate Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 229940100515 sorbitan Drugs 0.000 description 1
- 229950006451 sorbitan laurate Drugs 0.000 description 1
- 239000001593 sorbitan monooleate Substances 0.000 description 1
- 235000011069 sorbitan monooleate Nutrition 0.000 description 1
- 229940035049 sorbitan monooleate Drugs 0.000 description 1
- 239000001570 sorbitan monopalmitate Substances 0.000 description 1
- 235000011071 sorbitan monopalmitate Nutrition 0.000 description 1
- 229940031953 sorbitan monopalmitate Drugs 0.000 description 1
- 239000001587 sorbitan monostearate Substances 0.000 description 1
- 235000011076 sorbitan monostearate Nutrition 0.000 description 1
- 229940035048 sorbitan monostearate Drugs 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 125000004079 stearyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 229960002415 trichloroethylene Drugs 0.000 description 1
- UBOXGVDOUJQMTN-UHFFFAOYSA-N trichloroethylene Natural products ClCC(Cl)Cl UBOXGVDOUJQMTN-UHFFFAOYSA-N 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Water Supply & Treatment (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Processing Of Solid Wastes (AREA)
- Materials Engineering (AREA)
- Soil Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Treatment Of Biological Wastes In General (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
The present invention relates to microorganism compositionss.The presence of microorganism compositionss is placed on the situation of outdoor together with transport container after being transported to soil sanitation scene, the phenomenon of crystallization occurs in transport container when being placed several weeks.A kind of microorganism compositionss it is characterised in that described microorganism can purification soil, the organochlorine compound in subsoil water, described microorganism compositionss are by the sugar alcohol selected from more than a kind in Sorbitol, mannitol, xylitol；Glycerol；Nonionic surfactant；And water is constituted, the containing ratio of aforementioned sugar alcohol is less than 6 times of aforementioned glycerol, even if described microorganism compositionss are placed together with transport container in outdoor, also will not be in container intercrystalline.
Description
Technical field
The present invention relates in order to using the organochlorine compound in microbial decomposition soil and groundwater come purification soil and
Subsoil water and be injected into the microorganism compositionss in soil and groundwater.
Background technology
Because being by the problem of the soil and groundwater of chemical contamination, not only breaking up the mankind, also to destroy biosphere whole
The balance of body, from the viewpoint of environmental conservation, needs certain countermeasure.In addition, contaminated soil and subsoil water are put no
Reason, is related to that mankind's activity scope is restricted, is also required to countermeasure from economic point of view.
In the past, in order to purify by the soil and groundwater of chemical contamination it is proposed that lime method, Powder by Iron Powder, soil are opened
Dig the various methods such as cushion, soil wet soil formula ablution, biological degradation method.The pollution great majority of soil and groundwater are big model
Enclose the situation of diffusion, change the scale of method of the earth that bankets itself and expense becomes huge.
Generally acknowledged as do not need large-scale engineering and in original place economically one of method of purification soil and subsoil water
Biological degradation method is suitable.It is to make aerobism in soil and groundwater or anaerobic microbial decomposition as pollutant
The method of the chemical substance of matter.
For example, the microorganism of fusobacterium in anaerobism atmosphere decomposing organic matter and be decomposed into acetic acid etc..Release in this operation
Hydrogen.This hydrogen can replace chloroform, dichloromethane, dichloroethanes, the chlorine of trichloro ethylene such organochlorine based compound, thus only
Change these chemical substances.That is, can be to organochlorine based compound using the reduction reaction that the hydrogen producing under anaerobism atmosphere causes
Carry out dechlorination to purify chemical substance.
Propose and produced hydrogen as above and provide the material group of hydrogen to be referred to as hydrogen donor, and the same of activation of microorganism will be made
The material group (hereinafter referred to as " nutrient source ") of Shi Chengwei hydrogen donor is injected into the method in soil and groundwater.Such nutrient source
The worry of the secondary pollution that the part beyond being diffused into impurely when there is excessive injection is led to.Thus, for example patent documentation 1
In disclose make straight chain fatty acid be mixed into make granular straight chain fatty acid, in glycerol so that it is not easy to move from injection place
Dynamic compositionss out.
It is well known, however, that microorganism can inactivate it has to by the nutrition after extreme dilution under the nutrient source of high concentration on the contrary
Source is sent in soil and groundwater.If in addition, nutrient source is also close to natural materials, small toxicity and without having to worry about secondary dirt
Dye.Then it is proposed that viscosity is low, in soil and underground on the contrary compared with not from embedded the solidss being moved, gel
The method spreading in water and widely providing nutrient source from 1 well.
Patent Document 2 discloses by the nutrient source based on Sorbitol be diluted to 2000ppm and send into soil and
So that the technology of microbial decomposition organochlorine compound in subsoil water.More specifically, by Sorbitol 60%, glycerol 10%,
Anion 2%, the nutrient source of water 28% are diluted to 2000ppm and send into and so that it is spread in soil and groundwater.End in diffusion
End, the concentration of carbon in nutrient source is diluted to about 100ppm, for microorganism, becomes and jointly can absorb with chemical substance
The concentration of degree.Therefore, the decomposition of chemical substance can be promoted.
Patent Document 3 discloses in order that the nutrient source after diluting fully can diffuse to underground and add nonionic
The nutrient source of surfactant.Nutrient source herein comprises polyhydric alcohol and surfactant, and foregoing polyols are 62～68 weight
Part selected from Sorbitol, mannitol, more than a kind of sugar alcohol of xylitol and the glycerol of 8～10 weight portions, aforementioned surfaces are lived
Property agent is the nonionic surfactant of 1～5 weight portion, also comprises the water of 22～25 weight portions.
Prior art literature
Patent documentation
Patent documentation 1：Japanese Unexamined Patent Publication 2002-370085 publication (Japanese Patent Publication No. 3746726)
Patent documentation 2：Japanese Unexamined Patent Publication 2009-11939 publication (Japanese Patent Publication No. 5023850)
Patent documentation 3：Japanese Patent Publication No. 5587453
Content of the invention
Problems to be solved by the invention
The nutrient source (microorganism compositionss) with the composition of patent documentation 3 is actually applied, and has enumerated as made a reservation for
Such effect.Such nutrient source is prepared in the factory in advance and is filled 18 liters of tanks (so-called " a pipeful of tank "：Hereinafter referred to as
" transport container ") and it is transported to scene.The purification of soil needs the time of several weeks to several months, therefore to put into the transport of nutrient source
Container great majority are for being placed on the situation of outdoor.
Then, create the problem that nutrient source solidifies in transport container, is difficult to take out from transport container.
For solution to problem
The present invention be in view of above-mentioned problem and expect, also will not coagulate in transport container even if providing and being placed on outdoor
Solid microorganism with compositionss (nutrient source).
More specifically, the microorganism compositionss of the present invention are characterised by, described microorganism can purification soil,
Organochlorine compound in lower water, described microorganism compositionss by
Sugar alcohol selected from any more than a kind in Sorbitol, mannitol；
Glycerol；
Nonionic surfactant；And
Water is constituted,
The containing ratio of aforementioned sugar alcohol is less than 6 times of aforementioned glycerol.
The effect of invention
The thermal shock even if the microorganism compositionss of the present invention are subject to repetition also will not solidify in transport container.Therefore,
Transport to behind scene until use when manage on save worry.
Specific embodiment
Below the microorganism compositionss of the present invention are illustrated.Hereinafter illustrate exemplified with embodiments of the present invention and
A part for embodiment, the invention is not restricted to following explanation.Purport without departing from the present invention is it is possible to following embodiment party
Formula is changed.
The purpose of the microorganism compositionss of the present invention is, comprises for the microorganism in activating soil and subsoil water
Material, so that it spreads from injection.Therefore, it is set to liquid and the low state of viscosity.In addition, for respect to soil and ground
The purpose that microorganism in lower water is used with the state of highly diluted, needs for water solublity.In addition, it is net for soil and groundwater
The purpose changed, needs to play the function of nutrient source.
As such material, can be suitably by the use of the Sorbitol in the sugar alcohol as polyhydric alcohol.But, equally
Mannitol can be used.Sorbitol is illustrated herein.
In order that the Sorbitol being diluted with water keeps in soil and groundwater and mixes wetting agent.As moisturizing
Agent, can be suitably by the use of the glycerol as 3 yuan of alcohol.This is because glycerol itself can become the nutrient source of microorganism.In addition, by
In freezing point can be reduced in the same manner as ethylene glycol, therefore also there is the effect as anti-icing fluid.This is net in the low region of temperature
It is useful for changing during soil and groundwater.
Even if in addition, also not solidifying in order under preserving in outdoor placement, for the microorganism combination before dilution
Thing, contains glycerol with glycerol with respect to the ratio that Sorbitol is more than 1/6.Embodiment afterwards also would indicate that, as
If the Sorbitol of polyhydric alcohol is placed with high concentrations more than 60 mass %, sometimes it is subject to the temperature change of surrounding, in transport
Form crystal seed in container.And, sometimes centered on this crystal seed, produce crystallization and lead to solidify.
However, by the content reducing concentration or increase glycerol, it can be avoided that this phenomenon.Therefore, except Sorbitol
Beyond the mixed proportion of glycerol, preferably the content of Sorbitol is adjusted to below more than 53 mass % and 58 mass %.
The microorganism compositionss of the present invention can also contain water with the ratio below 40 mass % degree.As basic
Nutrient source is not need water.But, this is because it is also requisite that water is used for concentration regulation of nutrient source etc..Need
Bright, water can also as Sorbitol, glycerol, other additive surplus.
In addition, the water being used preferably implements the water after sterilization treatment.This is because, contain fish moldss etc. in water miscellaneous
Bacterium, Sorbitol also can become the nutrient source of these microorganisms, if therefore containing miscellaneous bacteria, causes microorganism to use during preserving
The problem of compositionss corrosion.
In addition, sterilization treatment can also be for boiling process it is also possible to irradiation ultraviolet radiation etc..Alternatively, it is also possible to using pure water,
This water initially not containing miscellaneous bacteria of ultra-pure water.
Nonionic surfactant is contained in nutrient source.When nutrient source is injected in soil and groundwater, surface
Activating agent can reduce the surface energy of soil, is easily wetted soil with respect to nutrient source.As a result, along with capillary effect
Really, nutrient source readily diffuses in soil and groundwater.Nonionic surfactant is unrelated with the pH of liquid and can play
Surface activity.
Therefore, nonionic surfactant does not rely on the pH in soil and groundwater.In soil and groundwater
Can be readily obtained anaerobic environment, therefore the anaerobic digestion aggravation of Organic substance and soil easily tends to acid.Even if in such ring
Under border, as long as having nonionic surfactant it becomes possible to make microorganism compositionss spread.
As nonionic surfactant, it is possible to use：Polyoxyethylene alkyl ether, i.e. polyoxyethylene lauryl ether, poly-
Oxygen ethylene cetyl ether, polyoxyethylene stearyl base ether, polyoxyethylene oleyl ether, polyoxyethylene higher alcohol ether, polyoxyethylene lima bean
Cool base ether, the distyrenated phenyl ether of polyoxyethylene etc.；Sorbitan fatty acid ester, i.e. Span-20,
Sorbitan monopalmitate, sorbitan monostearate, sorbitan monooleate, sorbitan distearyl
Acid esters etc.；Polyoxyethylene sorbitan fatty acid ester, i.e. polyoxyethylene sorbitol acid anhydride list coconut fatty acid ester, polyoxyethylene
Sorbitan laurate, polyoxyethylene sorbitan monopalmitate, polyoxyethylene sorbitan monostearate,
Polyoxyethylene sorbitan tristearate anhydride, polyoxyethylene sorbitol acid anhydride three isostearate, polyoxyethylene sorbitol
Acid anhydride monoleate, four Oleic acid polyoxyethylene sorbitol ethers etc..Wherein it is possible to suitably using polyoxyethylene lauryl ether.
And then, pH buffer can also be added in the microorganism compositionss of the present invention.PH buffer can be with respect to total
Amount interpolation 1～4 weight portion.When adding pH buffer, suitably adjust the ratio with other compositions.
PH buffer can be suitably used as has lactic acid series buffer agent, citric acid system buffer agent, phosphate buffer agent, acetic acid
It is buffer agent etc..They can add 1～4 weight portion.
Using as follows the reasons why pH buffer.As nutrient source microorganism compositionss by be injected into soil and ground
In lower water, being capable of microorganism in activating soil and subsoil water.However, when the microorganism after activation causes anaerobic digestion aggravation,
Generate the organic acid such as acetic acid, the pH in soil and groundwater reduces.If the pH of soil and groundwater excessively reduces, become to suppress
The activity of microorganism.That is, when microorganism compositionss being injected in soil and groundwater, certain period after the implantation is formed
Do not carry out the period of the decomposition of organochlorine compound etc..It is referred to as " lag phase of purification ".
PH buffer can somewhat relax the reduction of the pH that the generation of the organic acid such as acetic acid leads to, and can suppress pH
Excessively reduce " lag phase of purification " leading to.Therefore, " lag phase of purification " disappears.As a result, there is soil and groundwater
The advantage that period needed for purifying shortens.
Embodiment
The embodiment of the microorganism compositionss of the present invention is illustrated below.In order to confirm crystallization, prepare each shown in table 1
Sample, respectively fills 5 tanks in a transport container, applies temperature change in constant temperature test room.Transport container is common 18 liters of tanks,
For iron.The process of protecting film or screening glass is not especially set in inwall.In constant temperature test room, little with 24 DEG C 12
When, at 15 DEG C the circulation of 12 hours apply thermal shock in 30 days.It should be noted that in temperature chamber, the change of temperature is with greater need for about
60 minutes.
Sample 1 is Sorbitol 65 mass %, glycerol 10 mass %, nonionic surfactant 2 mass %, water 23 matter
Amount %.Nonionic surfactant uses polyoxyethylene lauryl ether.In addition, water is using the tap water through boiling.Thereafter
All sample all sames.Crystallization is created for 5 tanks in sample 1,5 tank.Show the result in table 1.
Sample 2 is Sorbitol 60 mass %, glycerol 10 mass %, nonionic surfactant 2 mass %, water 28 matter
Amount %.Crystallization is created for 5 tanks in sample 2,5 tank.Show the result in table 1.
Sample 3 is Sorbitol 59 mass %, glycerol 10 mass %, nonionic surfactant 2 mass %, water 29 matter
Amount %.Crystallization is not all produced for sample 3,5 tank.Show the result in table 1.
Sample 4 is Sorbitol 58 mass %, glycerol 10 mass %, nonionic surfactant 2 mass %, water 30 matter
Amount %.Crystallization is not all produced for sample 4,5 tank.Show the result in table 1.
Sample 5 is Sorbitol 55 mass %, glycerol 10 mass %, nonionic surfactant 2 mass %, water 33 matter
Amount %.Show the result in table 1.Crystallization is not all produced for sample 5,5 tank.Show the result in table 1.
[table 1]
| Test piece number (Test pc No.) | 1 | 2 | 3 | 4 | 5 |
| Sorbitol | 65 | 60 | 59 | 58 | 55 |
| Glycerol | 10 | 10 | 10 | 10 | 10 |
| Surfactant | 2 | 2 | 2 | 2 | 2 |
| Water | 23 | 28 | 29 | 30 | 33 |
| Result | 5/5 | 5/5 | 0/5 | 0/5 | 0/5 |
The unit of the numeral of each sample is quality %
Result is to confirm the tank number of crystallization in 5 tanks
With reference to table 1, when Sorbitol is more than 59 mass %, in 5 tanks of each sample, more than half creates crystallization.So
And, if 58 mass %, then there is not the tank producing crystallization in 5 tanks.As is well- known, the crystal of Sorbitol can lead to
Cross and be once cooled to extremely low temperature and lentamente rise high-temperature to obtain.However, do not carry out such Temperature Treatment herein.
Accordingly, it is difficult to think the crystal producing Sorbitol purely thus it becomes crystal seed and crystallization occurs.Permissible
Think the border of the liquid level of wall in transport container for the minute impurities being probably to be mixed into when preparing and microorganism compositionss
Part is formed doubtful crystal seed and is produced crystallization with it as reason.The microorganism filled in transport container is combined herein
The phenomenon of thing solidification is referred to as crystallization.
Then, it is 60 mass % and 58 mass % not causing crystallization for the Sorbitol causing crystallization, attempt
The content changing glycerol.The composition of each sample is shown in table 2.
In sample 6, Sorbitol is 60 mass %, glycerol is 7 mass %, nonionic surfactant is 2 mass %,
Water is 31 mass %.
In sample 7, Sorbitol is 58 mass %, glycerol is 7 mass %, nonionic surfactant is 2 mass %,
Water is 33 mass %.
In sample 8, Sorbitol is 60 mass %, glycerol is 8 mass %, nonionic surfactant is 2 mass %,
Water is 30 mass %.
In sample 9, Sorbitol is 58 mass %, glycerol is 8 mass %, nonionic surfactant is 2 mass %,
Water is 32 mass %.
In sample 10, Sorbitol is 60 mass %, glycerol is 9 mass %, nonionic surfactant is 2 matter
Amount %, water are 29 mass %.
In sample 11, Sorbitol is 58 mass %, glycerol is 9 mass %, nonionic surfactant is 2 matter
Amount %, water are 31 mass %.
In sample 12, Sorbitol is 60 mass %, glycerol is 11 mass %, nonionic surfactant is 2 matter
Amount %, water are 27 mass %.
In sample 13, Sorbitol is 58 mass %, glycerol is 11 mass %, nonionic surfactant is 2 matter
Amount %, water are 29 mass %.
In sample 14, Sorbitol is 60 mass %, glycerol is 12 mass %, nonionic surfactant is 2 matter
Amount %, water are 26 mass %.
In sample 15, Sorbitol is 58 mass %, glycerol is 12 mass %, nonionic surfactant is 2 matter
Amount %, water are 28 mass %.
In sample 16, Sorbitol is 65 mass %, glycerol is 11 mass %, nonionic surfactant is 2 matter
Amount %, water are 22 mass %.Show the result in table 2.
[table 2]
With reference to table 2, even thought sample 12 and 14 (Sorbitol 60 matter that produce crystallization by the result of table 1
Amount %), 5 tanks in 5 tanks all do not produce crystallization.In addition, for thinking sample 7, the 9,11 (mountain that should be unable to produce crystallization
Pears sugar alcohol 58 mass %), 1 tank in 5 tanks or 2 tanks create crystallization.Even in addition, being judged as not producing the fortune of crystallization
Defeated container, wherein also creates the block of fist size degree.
Can be drawn a conclusion by result above, the presence or absence of crystallization generation is not only relevant with the concentration of Sorbitol.In
It is then to be conceived to the ratio of Sorbitol and glycerol.Table 2 shows that Sorbitol (is expressed as " S with respect to the ratio of glycerol：
G”).
This Sorbitol is compared with respect to the ratio of glycerol, can predict and occur with 6 to 1 for boundary generation knot
The situation of crystallization.Then, sample 16 is the content of the Sorbitol of sample 1 (Sorbitol is 65 mass %) shown in table 1, tastes
Try the composition of the glycerol adding 11 mass %.In sample 16, Sorbitol is 5.9 to 1 with respect to glycerol.As shown in table 2, try
In sample 16, even if Sorbitol is 65 mass %, as long as with respect to glycerol with less than 6 times of ratio, then 5 tanks in 5 tanks are not all
Crystallization can occur.
As can seen above, if Sorbitol and the ratio of glycerol are less than 6 to 1 (if Sorbitol in microorganism compositionss
Few), then it can be avoided that crystallization.
Because Sorbitol is closely similar with the structure of mannitol, therefore mannitol also creates same phenomenon.
With regard to Sorbitol, based on the opinion obtaining in above-mentioned experiment with the composition hit and miss experiment of table 3 to determine whether crystallization.
Sample 17 is mannitol 60 mass %, glycerol 7 mass %, nonionic surfactant 2 mass %, water 31 matter
Amount %.Mannitol (is expressed as " M in table 3 with the ratio of glycerol：G ") it is 8.5：1.
Sample 18 is mannitol 58 mass %, glycerol 9 mass %, nonionic surfactant 2 mass %, water 31 matter
Amount %.Mannitol (is expressed as " M in table 3 with the ratio of glycerol：G ") it is 6.4：1.
Sample 19 is mannitol 60 mass %, glycerol 11 mass %, nonionic surfactant 2 mass %, water 27
Quality %.Mannitol (is expressed as " M in table 3 with the ratio of glycerol：G ") it is 5.4：1.
Sample 20 is mannitol 58 mass %, glycerol 11 mass %, nonionic surfactant 2 mass %, water 29
Quality %.Mannitol (is expressed as " M in table 3 with the ratio of glycerol：G ") it is 5.3：1.
Sample 21 is mannitol 65 mass %, glycerol 11 mass %, nonionic surfactant 2 mass %, water 22
Quality %.Mannitol (is expressed as " M in table 3 with the ratio of glycerol：G ") it is 5.9：1.
Show the result in table 3.With reference to table 3, mannitol is less than 6 with the ratio of glycerol：When 1, (mannitol is few or sweet
Oil is many), do not produce crystallization.It should be noted that for sample 18, crystallization produces in 4 tanks in 5 tanks.Itself and Pyrusussuriensiss
Sample 11 in the case of sugar alcohol is identical ratio of components, and in the case of Sorbitol, 1 tank in 5 tanks finds crystallization.Cause
This, be that mannitol is more prone to crystallization than Sorbitol.
[table 3]
| Test piece number (Test pc No.) | 17 | 18 | 19 | 20 | 21 |
| Mannitol | 60 | 58 | 60 | 58 | 65 |
| Glycerol | 7 | 9 | 11 | 11 | 11 |
| Surfactant | 2 | 2 | 2 | 2 | 2 |
| Water | 31 | 31 | 27 | 29 | 22 |
| Result | 5/5 | 4/5 | 0/5 | 0/5 | 0/5 |
| M∶G | 8.5∶1 | 6.4∶1 | 5.4∶1 | 5.3∶1 | 5.9∶1 |
The unit of the numeral of each sample is quality %
Result is to confirm the tank number of crystallization in 5 tanks
The embodiment of the clean-up effect of soil of microorganism compositionss for confirm the present invention is illustrated below.Embodiment
Carry out at the place polluted by tetrachloroethylene.Band water layer is 2.0～12.2m away from ground surface.In addition, four chloroethenes in subsoil water
Alkene concentration is about 0.8mg/L.It is provided with the injection well of a diameter of 100mm in test, in well head connecting tube, carry out microorganism group
The injection of compound.
In addition, in the local setting inspection well away from injection well 3m, termly carry out tce concentration in subsoil water, mountain
Pears sugar alcohol concentration, the monitoring of pH.In addition, the injection well injecting each microorganism compositionss is set with inspection well with being adequately isolated
Put, so that inspection well is not affected by each microorganism compositionss.
Each microorganism compositionss to be injected are using the aqueous solution being diluted to 2000ppm, continuous with 9.0L/ minute respectively
After injection 7 days, simply continue to be monitored.
Embodiment 1 is by Sorbitol 52 mass %, glycerol 9 mass %, nonionic surfactant 2 mass % and water
The mixture that 37 mass % mix.Sorbitol is " 5.7 with the ratio of glycerol：1”.It should be noted that as non-from
Sub- property surfactant, using polyoxyethylene lauryl ether.Water uses the common boiling tap water boiling water of 10 minutes
It is cooled to 25 DEG C of water.Composition and result are shown in table 4.
It should be noted that " G ratio " is " with respect to the ratio of glycerol " in table 4, glycerol is set to sugar alcohol when 1 by expression
Ratio.In addition, " S " represents Sorbitol in table 4, " M " represents mannitol.
Embodiment 2 be by Sorbitol 55 mass %, glycerol 10 mass %, nonionic surfactant 2 mass % and
The mixture that water 33 mass % mixes.Sorbitol is " 5.5 with the ratio of glycerol：1”.Embodiment 3 is by Sorbitol
The mixture that 58 mass %, glycerol 10 mass %, nonionic surfactant 2 mass % and water 30 mass % mix.
Sorbitol is " 5.8 with the ratio of glycerol：1”.
Embodiment 4 is by mannitol 52 mass %, glycerol 9 mass %, nonionic surfactant 2 mass % and water
The mixture that 37 mass % mix.Mannitol is " 5.7 with the ratio of glycerol：1”.
Embodiment 5 is by mannitol 55 mass %, glycerol 10 mass %, nonionic surfactant 2 mass %, water
The mixture that 33 mass % mix.Mannitol is " 5.5 with the ratio of glycerol：1”.Embodiment 6 is by mannitol 58
The mixture that quality %, glycerol 10 mass %, nonionic surfactant 2 mass % and water 30 mass % mix.Sweet
Dew sugar alcohol is " 5.8 with the ratio of glycerol：1”.
The sugar alcohol of the microbial composite of all of embodiment is 6 with respect to glycerol：Less than 1 ratio (sugar alcohol is few).
Composition and result are shown in table 4.It should be noted that when carrying out this embodiment, for the micro- life being filled into transport container
The thing longer situation of compositionss is to carry out outdoor placement more than 1 month in test site, but 1 tank does not all occur crystallization.
[table 4]
With reference to table 4, resolution ratio is obtained by the concentration and the concentration after 30 days of the tetrachloroethylene before testing, is represented with %.Separately
Outward, the concentration (mg/L) of the sugar behind 7 days at the sampling spot away from well 3m is shown.
In embodiment 1～6, the tetrachloroethylene at place away from well 3m has all decomposed more than 95%.In addition, taking away from well 3m
The concentration of the sugar after 7 days at sample place is more than 800mg/L.It follows that the composition of the microorganism compositionss of the present invention with
The state of highly diluted improves the wettability of soil surface, while being diffused into underground, contributes to the decomposition of tetrachloroethylene.
Industrial applicability
The microorganism compositionss of the present invention as using the microorganism in soil and groundwater by soil and groundwater
Organochlorine compound decompose, purify when nutrient source and suitably use.
Claims (2)
1. a kind of microorganism compositionss are it is characterised in that described microorganism being capable of purification soil, the organic chloride in subsoil water
Compound, described microorganism compositionss by
Sugar alcohol selected from any more than a kind in Sorbitol, mannitol；
Glycerol；
Nonionic surfactant；And
Water is constituted,
The containing ratio of described sugar alcohol is less than 6 times of described glycerol,
Described sugar alcohol is below more than 53 mass % and 58 mass % in described microorganism compositionss.
2. microorganism compositionss according to claim 1 are it is characterised in that described nonionic surfactant is poly-
Oxygen ethylene lauryl ether.
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| JP2007083169A (en) * | 2005-09-22 | 2007-04-05 | Miyoshi Oil & Fat Co Ltd | Decontaminating agent for soil and ground water |
| JP5023850B2 (en) * | 2007-07-05 | 2012-09-12 | パナソニック株式会社 | Method for purifying contaminated soil and groundwater |
| JP6195148B2 (en) * | 2013-03-28 | 2017-09-13 | パナソニックＩｐマネジメント株式会社 | Microbial nutrient source injection device |
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