CN105949273A - Preparation method for original antibacterial peptide serous fluid from housefly larvae - Google Patents
Preparation method for original antibacterial peptide serous fluid from housefly larvae Download PDFInfo
- Publication number
- CN105949273A CN105949273A CN201610255722.0A CN201610255722A CN105949273A CN 105949273 A CN105949273 A CN 105949273A CN 201610255722 A CN201610255722 A CN 201610255722A CN 105949273 A CN105949273 A CN 105949273A
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- China
- Prior art keywords
- antibacterial peptide
- serous fluid
- preparation
- housefly
- housefly larvae
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/39—Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
- A61K2039/55511—Organic adjuvants
- A61K2039/55516—Proteins; Peptides
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Analytical Chemistry (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Immunology (AREA)
- Animal Behavior & Ethology (AREA)
- General Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention provides a preparation method for an original antibacterial peptide serous fluid from housefly larvae to overcome the technical problems of complexity of process and equipment, inactivation of a great number of active housefly larva bacteria in extract and the like in the prior art. The preparation method is characterized by comprising the following steps: a, cleaning housefly larvae and then soaking the housefly larvae in light salt brine; b, grinding the soaked housefly larvae with a stone mill so as to obtain a serous fluid; c, separating filter residue from the serous fluid so as to obtain the original antibacterial peptide serous fluid; d, subjecting the original antibacterial peptide serous fluid to split charging in containers having undergone high-temperature virus-killing and disinfection; and e, covering the containers and storing the containers under a low temperature or refrigeration condition for subsequent usage. The method is simple in process and equipment, easy to operate, labor-saving, time-saving, low in investment and high in benefits, can maximally preserve the vitality of active housefly larva bacteria and lays a good purity and quality foundation for subsequent extraction of an antibacterial peptide preparation. The original antibacterial peptide serous fluid from housefly larvae prepared in the invention can be directly used for extracting an antibacterial peptide adjuvant which is further used for preparing a variety of vaccine preparations containing the adjuvant.
Description
Technical field:
The invention belongs to fly larvae antibacterial peptide preparing technical field, be specifically related to a kind of fly larvae antibacterial peptide virgin pulp liquid system
Preparation Method.
Background technology:
Owing to fly larvae antibacterial peptide has significant immunoadjuvant function, therefore fly larvae antibacterial peptide has been developed that into epidemic disease
Seedling adjuvant is applied.Need the most before this to prepare fly larvae antibacterial peptide extracting solution.About this respect,
" the fly larvae antibacterial peptide extraction system " of CN103992369A bulletin, the and " fly of CN103908668A bulletin
Maggot antibacterial peptide adjuvant and preparation method thereof and containing the bacterin preparation of this adjuvant and purposes " be two examples therein.
True explanation, the method preparing this extracting solution at present mainly has two kinds: dry fly larvae is first passed through second by the first
Acid solution carries out homogenized, the more extracted step of liquid, centrifugal, filtration etc. excessively, obtains fly larvae thereafter antibacterial
Peptide acid extracting solution.It two is fresh fly larvae to first pass through colloid mill and pulverizer is processed and heats etc. processing,
Add stabilizer and antiseptic kind controls enzyme and becomes, then extract antibacterial peptide, and during extracting antibacterial peptide
The medicine of the stabilizer added and antiseptic kind must also be washed.Thus be not difficult to find out prior art it
Defect: not only process equipment is many and complicated, technology requires height, time-consuming, and cost of investment is big, Er Qiehuo
Person is due to device therefor high speed rotating, or owing to high temperature is killed virus, or owing to using the technique such as chemical agent
Process, the most easily makes the fly larvae competent cell in extracting solution wreck, i.e. causes a lot of active bacteria to inactivate,
Thus reduce the effect of antibacterial peptide, therefore also necessarily affect next step purity extracting antimicrobial peptide preparation and quality.
Summary of the invention:
It is an object of the invention to, for the defect of prior art, it is provided that prepared by a kind of fly larvae antibacterial peptide virgin pulp liquid
Method, the method not only technique, equipment are simple, processing ease, saving of work and time, and cost of investment is little, and
The vigor of fly larvae competent cell can be preserved to greatest extent, extract antimicrobial peptide preparation for next step and established good
Good purity and quality base.
Its technical solution is: a kind of fly larvae antibacterial peptide virgin pulp liquid preparation method, it is characterised in that include as
Lower step:
A, fly larvae is cleaned after with 2% saline soak 2 hours；
B, fly larvae stone grinder is ground pulping liquor；
C, by serosity body filtering residue isolated antibacterial peptide virgin pulp liquid；
D, antibacterial peptide virgin pulp liquid is sub-packed in through high temperature exterminating bacterium cool down after container in；
E, by container cover after low temperature or freezen protective standby.
Compared with prior art, owing to the present invention uses primary pulp preparation, and use stone grinder defibrination, do not make
With high-speed rotating equipment, the most do not use high temperature, the most do not use any chemical agent, therefore in fly larvae virgin pulp liquid
Fly larvae competent cell be not exposed to destroy, i.e. do not result in fly larvae virgin pulp liquid active bacteria inactivation, thus not
But ensure the effect of antibacterial peptide, and purity and the quality assurance of extracting antimicrobial peptide preparation for next step have established
Determine basis.It was verified that the method also has technique, equipment simply, processing ease, saving of work and time,
The plurality of advantages such as cost of investment is little, and productivity effect is high.
Detailed description of the invention:
Below by embodiment, the present invention is elaborated.
Embodiment: the virgin pulp liquid preparation method of a kind of fly larvae antibacterial peptide, is characterized in that being realized by following steps:
A, to take fly larvae some, cross after clear water is cleaned again with 2% light salt brine immersion 2 hours；
B, the fly larvae stone grinder after 2 hours will be soaked grind pulping liquor；
C, by serosity body through filter, the separation circuit that removes slag obtains antibacterial peptide virgin pulp liquid；
D, antibacterial peptide virgin pulp liquid is sub-packed in advance in glass containers after high temperature exterminating bacterium cools down；
E, by glass containers cover after low temperature or freezen protective standby.
The fly larvae antibacterial peptide virgin pulp liquid prepared according to the present invention, can be used directly to extract antibacterial peptide adjuvant with new
Exploration project, and then be used for developing preparing the various bacterin preparation containing this adjuvant.
Claims (1)
1. a fly larvae antibacterial peptide virgin pulp liquid preparation method, it is characterised in that comprise the steps:
A, fly larvae is cleaned after with 2% saline soak 2 hours；
B, the fly larvae stone grinder after saline soak 2 hours is ground pulping liquor；
C, by serosity body filtering residue isolated antibacterial peptide virgin pulp liquid；
D, antibacterial peptide virgin pulp liquid is sub-packed in through high temperature exterminating bacterium cool down after container in；
E, by container cover after low temperature or freezen protective standby.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610255722.0A CN105949273A (en) | 2016-04-25 | 2016-04-25 | Preparation method for original antibacterial peptide serous fluid from housefly larvae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610255722.0A CN105949273A (en) | 2016-04-25 | 2016-04-25 | Preparation method for original antibacterial peptide serous fluid from housefly larvae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105949273A true CN105949273A (en) | 2016-09-21 |
Family
ID=56916001
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610255722.0A Pending CN105949273A (en) | 2016-04-25 | 2016-04-25 | Preparation method for original antibacterial peptide serous fluid from housefly larvae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105949273A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107142186A (en) * | 2017-07-10 | 2017-09-08 | 罗乌支 | A kind of preparation method of the sub- native peptides wine of narcissus |
| CN107183459A (en) * | 2017-07-10 | 2017-09-22 | 罗乌支 | A kind of preparation method of narcissus homogenate peptide liquid |
| CN108850529A (en) * | 2018-06-07 | 2018-11-23 | 苏州康尧环保科技有限公司 | A kind of fly maggot disinfection cleaning process flow and disinfection cleaning system |
| CN110367399A (en) * | 2019-07-02 | 2019-10-25 | 佛山市顺德区旺海饲料实业有限公司 | A kind of largemouth bass scrod swelling feed and preparation method thereof improving mark shoot survival percent |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102558387A (en) * | 2010-12-13 | 2012-07-11 | 青岛中仁药业有限公司 | Method for extracting chitin and antibacterial peptide from fly larvae |
-
2016
- 2016-04-25 CN CN201610255722.0A patent/CN105949273A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102558387A (en) * | 2010-12-13 | 2012-07-11 | 青岛中仁药业有限公司 | Method for extracting chitin and antibacterial peptide from fly larvae |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107142186A (en) * | 2017-07-10 | 2017-09-08 | 罗乌支 | A kind of preparation method of the sub- native peptides wine of narcissus |
| CN107183459A (en) * | 2017-07-10 | 2017-09-22 | 罗乌支 | A kind of preparation method of narcissus homogenate peptide liquid |
| CN108850529A (en) * | 2018-06-07 | 2018-11-23 | 苏州康尧环保科技有限公司 | A kind of fly maggot disinfection cleaning process flow and disinfection cleaning system |
| CN110367399A (en) * | 2019-07-02 | 2019-10-25 | 佛山市顺德区旺海饲料实业有限公司 | A kind of largemouth bass scrod swelling feed and preparation method thereof improving mark shoot survival percent |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160921 |