CN105943671A - Method for extracting effective ingredient from exocarpium citri - Google Patents
Method for extracting effective ingredient from exocarpium citri Download PDFInfo
- Publication number
- CN105943671A CN105943671A CN201610439562.5A CN201610439562A CN105943671A CN 105943671 A CN105943671 A CN 105943671A CN 201610439562 A CN201610439562 A CN 201610439562A CN 105943671 A CN105943671 A CN 105943671A
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- CN
- China
- Prior art keywords
- exocarpium citri
- citri rubrum
- effective component
- component extracting
- precipitate
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Abstract
The invention belongs to the medicine field, and particularly relates to a method for extracting an effective ingredient from exocarpium citri. The method comprises the following steps that 1, exocarpium citri is taken, water vapor is introduced into exocarpium citri for heating, a 95% ethyl alcohol solvent is added for extraction, and filtering is conducted to obtain filtrate; 2, ethyl alcohol is recycled from the filtrate until the filtrate is an extract free of alcohol smell, the relative density of the extract is regulated to be 1.10-1.15 at 70 DEG C by adding water, standing is conducted, filtering is conducted, and a precipitate is collected; 3, the precipitate is dried to obtain a exocarpium citri flavones extract. The method is little in used ethyl alcohol solvent, low in cost, good in extraction effect, high in efficiency, simple in process and suitable for industrialized production.
Description
[technical field]
The present invention relates to field of medicaments, be specifically related to a kind of method of effective component extracting from Exocarpium Citri Rubrum.
[background technology]
Exocarpium Citri Rubrum, calls, river Exocarpium Citri Rubrum red into rue skin, rue, for rutaceae orange Citrus reticulata Blanco and
The dry outside rind of variety, warm in nature, acrid in the mouth, hardship, return lung, spleen channel, cold expelling dampness, activating QI to eliminate phlegm, alleviating distention in middle-JIAO spleen invigorating.
Cure mainly cough due to wind and cold's abundant expectoration QI rising in reverse order, nausea and vomiting, breast institute painful abdominal mass is swollen the moon.
" medicine origin " " reason in the heart, lung qi " Compendium of Material Medica " therapeutic method to keep the adverse QI flowing downwards expectorant " " book on Chinese herbal medicine converges " " can be delivered except trembling with fear "
" talking whinchat test side " controls " pained in Tu " " plucking unit side " " controlling wind-phlegm numb ", and " married woman's good recipe " " after managing property, temper is not
Profit, urinary obstruction " Holy Benevolent Prescriptions " controls acute mastitis, do not ties the most scattered, tied the most routed, the most unbearably painful person " " book on Chinese herbal medicine meets former " " Fructus Citri tangerinae
Red and expert main lung cold cough excessive phlegm, deficient side multiplex it, right chronic cough diarrhea due to stagnation of QI, the most non-preferably " " medical center bun is wanted " " Exocarpium Citri Rubrum specially enters lung,
Hold concurrently to deliver, go Intradermal white, lighter empty floating, also remove pathogen of the lung ear ".
Exocarpium Citri Rubrum contains the multiple flavonoids such as Hesperidin, neohesperidin, and flavone compound (Flavonoids) is one
Planting the polyphenol compound being distributed widely in nature, because the multiformity of structure, this compounds has multiple biological activity,
Such as antioxidation, lowering blood pressure and blood fat, anticancer etc., modern pharmacological research shows, Hesperidin has the effects such as maintenance osmotic pressure,
Can be used for the auxiliary treatment of cardiovascular system diseases.
" research of Exocarpium Citri Rubrum total flavone extracting process " (Yang Shangyi, Dong Mei, Pan Zhiran, " China's prescription drugs ", 2015 years 08
Phase) disclose the extracting method of a kind of Exocarpium Citri Rubrum total flavones, it uses solid-liquid ratio to be 1:10, and Extraction solvent is 70% ethanol, extracts
Temperature 70 C, extracts 1h, extracts 2 times, and the extraction ratio of Exocarpium Citri Rubrum total flavones is 5.28% with this understanding.In big production, should
Method not only uses organic solvent amount big, and cost is high and extraction ratio is low.
[summary of the invention]
The goal of the invention of the present invention is: for the problem of above-mentioned existence, it is provided that a kind of method of effective component extracting from Exocarpium Citri Rubrum,
Described method uses alcohol solvent few, low cost, and extraction effect is good, and efficiency is high；Technique is simple, is suitable for industrialized production.
To achieve these goals, the technical solution used in the present invention is as follows:
A kind of method of effective component extracting from Exocarpium Citri Rubrum, comprises the following steps:
(1) take Exocarpium Citri Rubrum, be passed through steam heating, be subsequently adding 95% alcohol solvent and extract, filter, obtain filtrate；
(2) filtrate recycling ethanol is to the extractum without alcohol taste, adds water move to relative density 1.10～1.15 when 70 DEG C, stands, mistake
Filter, collects precipitate；
(3) precipitate is dried and is obtained Exocarpium Citri Rubrum flavone extract.
Further, the described method of effective component extracting from Exocarpium Citri Rubrum, in step (1), the water vapour being passed through is 120 DEG C
Saturated vapor, described heat time heating time is 5-10 minute.
Further, the described method of effective component extracting from Exocarpium Citri Rubrum, in step (1), described addition 95% alcohol solvent
The time extracted is 20-30 minute.
Further, the described method of effective component extracting from Exocarpium Citri Rubrum, in step (1), water vapour and the weight of 95% ethanol
Amount ratio is 1:6-1:7.
Further, the described method of effective component extracting from Exocarpium Citri Rubrum, in step (1), described water vapour and 95% ethanol
The weight ratio of total addition and Exocarpium Citri Rubrum be 3:1-4:1.
Further, the described method of effective component extracting from Exocarpium Citri Rubrum, in step (2), described time of repose is 48 little
More than time.
In sum, owing to have employed technique scheme, the invention has the beneficial effects as follows:
(1) Exocarpium Citri Rubrum extract before be passed through steam heating, make moisture content rapidly permeate in medical material, the cell outer surface of Exocarpium Citri Rubrum will deliquescing,
And gradually expand, be at this moment originally deposited in intracellular effective ingredient with crystallization or setting, will quickly with the medical material entered inside
Moisture combine and soluble in water, form the tissue solution of the internal high concentration of medical material, at this moment add 95% alcohol solvent, due to medicine
Inside and outside material cell tissue, solution concentration is different, forms obvious osmotic pressure difference, and the effective ingredient of the internal high concentration of medical material is the most rapidly
Ooze out and be dissolved in alcohol solvent.Overcoming in Conventional solvents extraction, solvent is difficult to penetrate into, and bubble then loss effective ingredient is scarce for a long time
Point, uses alcohol solvent few, low cost, and extraction effect is good, and efficiency is high.
(2) present invention can avoid the effective ingredient of Exocarpium Citri Rubrum to be destroyed by long-time heating as far as possible, improves extraction ratio.
(3) present invention process is simple, is suitable for industrialized production.
[detailed description of the invention]
Below in conjunction with embodiment, the present invention is described in detail, but they are not the further restrictions to the present invention.
Embodiment 1:
(1) taking Exocarpium Citri Rubrum medical material 1000 grams, the saturated vapor being passed through 120 DEG C heats 5 minutes, and being wherein passed through water vapour weight is
430g, is subsequently adding 95% alcohol solvent 2600g and carries out extracting 30 minutes, and 200 mesh nylon filtering cloths filter, and obtain filtrate；
(2) filtrate recycling ethanol is to the extractum without alcohol taste, adds water move to relative density 1.10 when 70 DEG C, stands 48 hours, mistake
Filter, collects precipitate；
(3) 60-70 DEG C of drying of precipitate obtains Exocarpium Citri Rubrum flavone extract.
Embodiment 2:
(1) taking Exocarpium Citri Rubrum medical material 1000 grams, the saturated vapor being passed through 120 DEG C heats 10 minutes, and being wherein passed through water vapour weight is
500g, is subsequently adding 95% alcohol solvent 3500g and carries out extracting 20 minutes, and 200 mesh nylon filtering cloths filter, and obtain filtrate；
(2) filtrate recycling ethanol is to the extractum without alcohol taste, adds water move to relative density 1.15 when 70 DEG C, stands 49 hours, mistake
Filter, collects precipitate；
(3) 60-70 DEG C of drying of precipitate obtains Exocarpium Citri Rubrum flavone extract.
Embodiment 3:
(1) taking Exocarpium Citri Rubrum medical material 1000 grams, the saturated vapor being passed through 120 DEG C heats 6 minutes, and being wherein passed through water vapour weight is
570g, is subsequently adding 95% alcohol solvent 3430g and carries out extracting 25 minutes, and 100 mesh nylon filtering cloths filter, and obtain filtrate；
(2) filtrate recycling ethanol is to the extractum without alcohol taste, adds water move to relative density 1.13 when 70 DEG C, stands 50 hours, mistake
Filter, collects precipitate；
(3) 60-70 DEG C of drying of precipitate obtains Exocarpium Citri Rubrum flavone extract.
Embodiment 4:
(1) taking Exocarpium Citri Rubrum medical material 1000 grams, the saturated vapor being passed through 120 DEG C heats 8 minutes, and being wherein passed through water vapour weight is
375g, is subsequently adding 95% alcohol solvent 2625g and carries out extracting 28 minutes, and 150 mesh nylon filtering cloths filter, and obtain filtrate；
(2) filtrate recycling ethanol is to the extractum without alcohol taste, adds water move to relative density 1.12 when 70 DEG C, stands 52 hours, mistake
Filter, collects precipitate；
(3) 60-70 DEG C of drying of precipitate obtains Exocarpium Citri Rubrum flavone extract.
Embodiment 5:
(1) taking Exocarpium Citri Rubrum medical material 1000 grams, the saturated vapor being passed through 120 DEG C heats 9 minutes, and being wherein passed through water vapour weight is
460g, is subsequently adding 95% alcohol solvent 3000g and carries out extracting 22 minutes, and 200 mesh nylon filtering cloths filter, and obtain filtrate；
(2) filtrate recycling ethanol is to the extractum without alcohol taste, adds water move to relative density 1.11 when 70 DEG C, stands 53 hours, mistake
Filter, collects precipitate；
(3) 60-70 DEG C of drying of precipitate obtains Exocarpium Citri Rubrum flavone extract.
Comparative example:
(1) taking Exocarpium Citri Rubrum medical material 1000 grams, add 70% ethanol extraction twice, add 70% ethanol weight is 5000 grams every time,
Extracting 1 hour, each Extracting temperature is 70 DEG C every time, and 100 mesh filter, and obtain filtrate；
(2) filtrate recycling ethanol is to the extractum without alcohol taste；
(3) 60-70 DEG C of drying of extractum obtains Exocarpium Citri Rubrum flavone extract.
The Exocarpium Citri Rubrum flavone extract extraction ratio that above example is prepared into see table.
As seen from table, the extraction efficiency of the present invention is higher.
Described above is the detailed description for the preferable possible embodiments of the present invention, but embodiment is not limited to the special of the present invention
Profit application range, the equal change completed under the technical spirit suggested by all present invention or modification change, the present invention all should be belonged to
Contained the scope of the claims.
Claims (6)
1. the method for an effective component extracting from Exocarpium Citri Rubrum, it is characterised in that comprise the following steps:
(1) take Exocarpium Citri Rubrum, be passed through steam heating, be subsequently adding 95% alcohol solvent and extract, filter, obtain filtrate；
(2) filtrate recycling ethanol is to the extractum without alcohol taste, adds water move to relative density 1.10～1.15 when 70 DEG C, stands, mistake
Filter, collects precipitate；
(3) precipitate is dried and is obtained Exocarpium Citri Rubrum flavone extract.
2. the method for effective component extracting from Exocarpium Citri Rubrum as claimed in claim 1, it is characterised in that: in step (1), logical
The water vapour entered is the saturated vapor of 120 DEG C, and described heat time heating time is 5-10 minute.
3. the method for effective component extracting from Exocarpium Citri Rubrum as claimed in claim 1, it is characterised in that: in step (1), institute
The time stating addition 95% ethonal extraction is 20-30 minute.
4. the method for effective component extracting from Exocarpium Citri Rubrum as claimed in claim 1, it is characterised in that: in step (1), water
The weight ratio of steam and 95% ethanol is 1:6-1:7.
5. the method for effective component extracting from Exocarpium Citri Rubrum as claimed in claim 1, it is characterised in that: in step (1), institute
The weight ratio of the total addition and Exocarpium Citri Rubrum of stating water vapour and 95% ethanol is 3:1-4:1.
6. the method for effective component extracting from Exocarpium Citri Rubrum as claimed in claim 1, it is characterised in that: in step (2), institute
Stating time of repose is more than 48 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610439562.5A CN105943671A (en) | 2016-06-20 | 2016-06-20 | Method for extracting effective ingredient from exocarpium citri |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610439562.5A CN105943671A (en) | 2016-06-20 | 2016-06-20 | Method for extracting effective ingredient from exocarpium citri |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105943671A true CN105943671A (en) | 2016-09-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610439562.5A Pending CN105943671A (en) | 2016-06-20 | 2016-06-20 | Method for extracting effective ingredient from exocarpium citri |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1852724A (en) * | 2003-09-19 | 2006-10-25 | 恩格哈德药物有限及两合公司 | Method for the production of a storage-resistant ivy leaf extract, and extract produced according to said method |
| CN101519416A (en) * | 2008-02-27 | 2009-09-02 | 中国科学院过程工程研究所 | Method for extracting and separating soluble functional components in hemp in a steam exploded manner |
| CN102716237A (en) * | 2012-06-29 | 2012-10-10 | 中山大学 | Method for extracting pummelo peel |
| CN103566001A (en) * | 2012-08-09 | 2014-02-12 | 河南科技学院 | Method for extracting flavones from poplar buds |
| CN103705600A (en) * | 2013-12-26 | 2014-04-09 | 晨光生物科技集团股份有限公司 | Method for producing green coffee bean extract |
-
2016
- 2016-06-20 CN CN201610439562.5A patent/CN105943671A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1852724A (en) * | 2003-09-19 | 2006-10-25 | 恩格哈德药物有限及两合公司 | Method for the production of a storage-resistant ivy leaf extract, and extract produced according to said method |
| CN101519416A (en) * | 2008-02-27 | 2009-09-02 | 中国科学院过程工程研究所 | Method for extracting and separating soluble functional components in hemp in a steam exploded manner |
| CN102716237A (en) * | 2012-06-29 | 2012-10-10 | 中山大学 | Method for extracting pummelo peel |
| CN103566001A (en) * | 2012-08-09 | 2014-02-12 | 河南科技学院 | Method for extracting flavones from poplar buds |
| CN103705600A (en) * | 2013-12-26 | 2014-04-09 | 晨光生物科技集团股份有限公司 | Method for producing green coffee bean extract |
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| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
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| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160921 |