CN108635445A - The application of rodgersflower rhizome flavones, Cortex Phellodendri flavones and Radix Angelicae Sinensis polysaccharide compound in preparing veterinary drug - Google Patents
The application of rodgersflower rhizome flavones, Cortex Phellodendri flavones and Radix Angelicae Sinensis polysaccharide compound in preparing veterinary drug Download PDFInfo
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- CN108635445A CN108635445A CN201810854614.4A CN201810854614A CN108635445A CN 108635445 A CN108635445 A CN 108635445A CN 201810854614 A CN201810854614 A CN 201810854614A CN 108635445 A CN108635445 A CN 108635445A
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- flavones
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/756—Phellodendron, e.g. corktree
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The invention discloses a kind of rodgersflower rhizome flavones, the application of Cortex Phellodendri flavones and Radix Angelicae Sinensis polysaccharide compound in preparing veterinary drug, and rodgersflower rhizome flavones crude extract, Cortex Phellodendri flavones crude extract and Radix Angelicae Sinensis polysaccharide crude extract are pressed 2:2:1 ratio forms Chinese herbal compounds, is applied to prepare the medicated premix in the veterinary drug of enhancing animal body immune function, and the drug concentration being added in veterinary drug is 1.25 ~ 5mg/mL；Effect is improved to the low rat blood serum medium size lymphocyte of immunogenicity and leucocyte content, Immunosuppreessed rats physical condition is made to be restored, shows that rodgersflower rhizome, Cortex Phellodendri flavones and Radix Angelicae Sinensis polysaccharide slightly carry compound and have the function of significantly increasing animal body immune function.
Description
Technical field
The invention belongs to animal medicine herbal medicine preparation field, specifically a kind of rodgersflower rhizome flavones, Cortex Phellodendri flavones and Radix Angelicae Sinensis are more
Application of the saccharide complex in preparing veterinary drug has the function of significantly increasing animal body immune function.
Background technology
Rodgersflower rhizome medicinal material is saxifragaceae plant, the dry rhizome that southwest ghost lamp is held up or leatherleaf ghost lamp is held up.Have in Chinese medicine
The effect of convergence, anti-inflammatory, dispelling wind and eliminating dampness, is usually used in treating dysentery, enteritis, cold headache, traumatic hemorrhage, treating rheumatic ostealgia, old age
The common diseases such as property bronchitis.Development and application at present in animal medicine are less.
Cortex Phellodendri medicinal material is the dry bark of rutaceae wampee, and bitter is cold in nature, returns kidney, wing moon bright warp, has heat-clearing
Eliminating dampness, purging intense heat, except steaming, detoxification sore treatment and other effects river, Chinese medicine be mainly used for treat damp-heat dysentery, yellow subcutaneous ulcer, band under, heat gonorrhea, anal fistula,
The diseases such as wound infection after night sweat, hectic fever due to yin labor heat, sore.Modern Chinese herbal medicine composition Study is analyzed, and alkaloid, Huang are contained in Cortex Phellodendri
The multiple biological activities ingredients such as ketone, phenols and Cortex Phellodendri lactones.Development and application at present in animal medicine are less.
Radix Angelicae Sinensis derives from the dry root of umbelliferae angelica, warm-natured, sweet in flavor, pungent, Return liver, the heart, the spleen channel.Angelica root enters
Medicine has the effect of replenishing and activating blood, menstruction regulating and pain relieving, relax bowel and regulate and control immunocompetence.Its main component have volatile oil, polysaccharide,
Polyphenol, flavones etc..In recent years, domestic and international medical expert study find Radix Angelicae Sinensis have anti anoxia, anticancer, the beauty of health skin, replenishing and activating blood,
Antibacterial, anti arteriosclerosis effect etc., to the deep development of Radix Angelicae Sinensis medicine, health products and cosmetic field achieve centainly at
Effect, research application is less in terms of animal medicine.
Flavones is a kind of Secondary Metabolite Production in Plants with 2- phenyl chromone structures, is that one kind is widely present in plant
In active material, medically have the function of anti-oxidant, antitumor, cardiovascular disease resistant and anti-inflammatory etc., have it is wide
Application prospect.Application of the Flavonoid substances in animal productiong at present is less.
The natural macromolecular substance that the aldose or ketose that polysaccharide is got up by glucosides key connection form is constitute life four
One of big base substance, universally present in plant.Studies have shown that polysaccharide and its complex molecule have a variety of biologies living
Property and important physiological action, be widely used in Animal husbandry production, to ensure livestock products safety and China's animal husbandry can hold
Supervention exhibition is of great significance.
Before the application proposes, rodgersflower rhizome, Cortex Phellodendri flavones and Radix Angelicae Sinensis polysaccharide compound are used in the medicine material work for preparing veterinary drug
For additive application there is not yet relevant report.
Cell factor and immunoglobulin that immunocyte generates are the signal of interest and substance of immunocyte interaction
Basis plays a significant role in Cellular Immunity and humoral immunity regulation and control.Immune cell types are more, widely distributed.Blood
In maturation immunity cell be commonly called as leucocyte, be mainly made of mononuclearcell, including monocyte, lymphocyte and grain are thin
Born of the same parents.Lymphocyte includes T, bone-marrow-derived lymphocyte, is most important immunocyte, and T lymphocytes participate in cellular immunity, bone-marrow-derived lymphocyte
Participate in humoral immunity.
Cyclophosphamide is usually used in antitumor treatment, and maximum side effect is exactly immunosupress, is especially exempted to body fluid
The inhibition of epidemic disease, so, cyclophosphamide is usually used in the foundation that animal immune inhibits model.
Invention content
The purpose of the present invention is to provide a kind of rodgersflower rhizome, Cortex Phellodendri flavones and Radix Angelicae Sinensis polysaccharide compound answering in preparing veterinary drug
With.
In order to realize the above-mentioned purpose of the present invention, the present invention provides the following technical solutions：
A kind of rodgersflower rhizome flavones, the application of Cortex Phellodendri flavones and Radix Angelicae Sinensis polysaccharide compound in preparing veterinary drug, which is characterized in that rodgersflower rhizome is yellow
Ketone crude extract, Cortex Phellodendri flavones crude extract and Radix Angelicae Sinensis polysaccharide crude extract press 2:2:1 quality proportioning forms compound, is used to prepare increasing
Medicated premix in the veterinary drug of strong animal body immune function, the rodgersflower rhizome flavones crude extract extract preparation from rodgersflower rhizome plant
It obtains, the Cortex Phellodendri flavones crude extract is extracted from Calusena lansium tree plant and is prepared, and the Radix Angelicae Sinensis polysaccharide crude extract is planted from Radix Angelicae Sinensis
Extraction is prepared in object, and the rodgersflower rhizome flavones crude extract, Cortex Phellodendri flavones crude extract and Radix Angelicae Sinensis polysaccharide crude extract form compound
For object as the medicated premix in veterinary drug, the medicated premix additive amount being added in veterinary drug is 1.25 ~ 5mg/mL.
2, rodgersflower rhizome flavones as described in claim 1, Cortex Phellodendri flavones and Radix Angelicae Sinensis polysaccharide compound answering in preparing veterinary drug
With, which is characterized in that the compound of the rodgersflower rhizome flavones crude extract, Cortex Phellodendri flavones crude extract and Radix Angelicae Sinensis polysaccharide crude extract composition
Preparation process is as follows：
One, the extraction of rodgersflower rhizome flavones：
（1）The rodgersflower rhizome rhizome dried is smashed it through into 40 mesh sieve, obtains rodgersflower rhizome powder；
（2）Rodgersflower rhizome powder is taken, by feed liquid mass ratio 1:（55-65）60% ethyl alcohol of concentration is added to impregnate, stands overnight, ultrasonic wave work(
10-20min is extracted under the conditions of rate 280-320W, 65-75 DEG C of water-bath 2.5-3.5 hours in water-bath；
（3）It is filtered with vacuum pump and removes the dregs of a decoction, then ethyl alcohol is flung to through depressurizing Rotary Evaporators, be put into 65-75 DEG C of thermostatic drying chamber
It is dry, obtain the rodgersflower rhizome flavones crude extract that purity content is 35-40%；
Two, the extraction of Cortex Phellodendri flavones：
（1）The bark for the wampee dried is smashed it through into 40 mesh sieve, obtains Cortex Phellodendri powder；
（2）Phellodendron powder is taken, by feed liquid mass ratio 1:（55-65）60% ethyl alcohol of concentration is added to impregnate, stands overnight, ultrasonic wave work(
10-20min is extracted under the conditions of rate 280-320W, in 65-75 DEG C of water-bath water-bath 2.5-3.5 hours；
（3）It is filtered with vacuum pump and removes the dregs of a decoction, then ethyl alcohol is flung to through depressurizing Rotary Evaporators, be put into thermostatic drying chamber control and exist
It is dry under the conditions of 65-75 DEG C, obtain the Cortex Phellodendri flavones crude extract that content is not less than 12%；
Three, the extraction of Radix Angelicae Sinensis polysaccharide：
（1）It takes the dry root of plant Radix Angelicae Sinensis to smash it through 20 mesh sieve, obtains angelica powder；
（2）Angelica powder is taken, by feed liquid mass ratio 1:（35-45）Distilled water is added, in 55-65 DEG C of water-bath and ultrasonic power 280-
10-20min is extracted under the conditions of 320W；
（3）The liquid that previous step obtains extracts 1h in 75-85 DEG C of hot water, is filtered after cooling and obtains concentrate, in concentrate
The ethyl alcohol of middle addition a concentration of 95%, makes ethanol content in concentrate reach 80%, is stood overnight under 4 DEG C of cold environmental conditions；
（4）The isolated precipitations of 1500r/min and supernatant in centrifuge, collection are dried under the conditions of being deposited in 60 DEG C, are obtained pure
The Radix Angelicae Sinensis polysaccharide that degree is 30%；
Four, gained rodgersflower rhizome flavones, Cortex Phellodendri flavones and Radix Angelicae Sinensis polysaccharide three are pressed 2:2:It is yellow that 1 quality proportioning forms rodgersflower rhizome, Cortex Phellodendri
Ketone and Radix Angelicae Sinensis polysaccharide compound.
Rodgersflower rhizome, Cortex Phellodendri flavones and the Radix Angelicae Sinensis polysaccharide compound, in pig lymphocyte conversion test and to rat
Display has the effect of significantly increasing animal body immune function in immunological regulation experiment, and with improve rat lymphocyte with
The effect of quantity of leucocyte makes Immunosuppreessed rats physical condition be restored.
Compared with prior art, the present invention has following excellent beneficial effects：
Rodgersflower rhizome flavones, Cortex Phellodendri flavones and Radix Angelicae Sinensis polysaccharide extraction process in the present invention are simple, extraction conditions are mild, gained compound
It is active strong, show that compound can be applied on animal in experiment；The compound that the immunological regulation experiment display of rat is described has
The effect of animal body immune function is significantly increased, easy exploitation is used at veterinary drug, has raw material sources are wide, improve immunity to show
Work, pharmaceutical safety and efficient advantage.
Description of the drawings
Fig. 1 is the influence situation of change of rodgersflower rhizome flavones, Cortex Phellodendri flavones and Radix Angelicae Sinensis polysaccharide compound to rat lymphocyte number
Schematic diagram.
Fig. 2 is that the influence situation of change of rodgersflower rhizome flavones, Cortex Phellodendri flavones and Radix Angelicae Sinensis polysaccharide compound to rat leukocyte number is shown
It is intended to.
Specific implementation mode
With reference to embodiment, the present invention is described in further detail.
It will be understood to those of skill in the art that the following example is merely to illustrate the present invention, and it should not be regarded as limiting this hair
Bright range.In the examples where no specific technique or condition is specified, according to technology or condition described in document in the art
Or it is carried out according to product description.Production firm person is not specified in material therefor or equipment, is that can be obtained by buying
Conventional products.
It is extracted in the extract flavone of " drug " and rodgersflower rhizome that occur in the application, the extract flavone and Radix Angelicae Sinensis of Cortex Phellodendri
The compound of Radix Angelicae Sinensis polysaccharide composition is same meaning.
The extraction of flavones in embodiment 1, rodgersflower rhizome
（1）The rodgersflower rhizome rhizome dried is smashed it through into 40 mesh sieve, obtains rodgersflower rhizome powder；
（2）Rodgersflower rhizome powder is taken, by solid-liquid ratio 1:60 add 60% ethyl alcohol of concentration to impregnate, and stand overnight, ultrasonic 15min, 70 DEG C of water-baths
Water-bath 3 hours；
（3）It is filtered with vacuum pump and removes the dregs of a decoction, then ethyl alcohol is flung to through depressurizing Rotary Evaporators, put 70 DEG C of thermostatic drying chamber dryings,
Obtain the rodgersflower rhizome flavones crude extract that 3.68g purity contents are 39.35%.
The extraction of flavones in embodiment 2, Cortex Phellodendri
（1）The bark for the wampee dried is smashed it through into 40 mesh sieve, obtains Cortex Phellodendri powder；
（2）Cortex Phellodendri powder is taken, by solid-liquid ratio 1:60 add 60% ethyl alcohol of concentration to impregnate, and stand overnight, ultrasonic 15min, 70 DEG C of water-bath water
Bath 3 hours；
（3）It is filtered with vacuum pump and removes the dregs of a decoction, then ethyl alcohol is flung to through depressurizing Rotary Evaporators, put 70 DEG C of thermostatic drying chamber dryings,
Obtain the Cortex Phellodendri flavones crude extract that 2.48g purity contents are 12%.
Radix Angelicae Sinensis polysaccharide extraction in embodiment 3, Radix Angelicae Sinensis
（1）It takes the dry root of plant Radix Angelicae Sinensis to smash it through 20 mesh sieve, obtains angelica powder；
（2）Take angelica powder, liquid ratio 1g:Distilled water is added in 40mL, ultrasonic extraction 15min under 60 DEG C of water bath conditions；
（3）1h is extracted in 80 DEG C of hot water, is filtered after cooling, a concentration of 95% ethyl alcohol is added in concentrate, makes second in concentrate
Alcohol content reaches 80%, is stood overnight under 4 DEG C of cold environmental conditions；
（4）It controls rotating speed 1500r/min in centrifuge to be detached, precipitation and supernatant, collection are dried under the conditions of being deposited in 60 DEG C
It is dry, obtain the Radix Angelicae Sinensis polysaccharide extract that 5.65g purity contents are 30% or so.
（5）Gained rodgersflower rhizome flavones, Cortex Phellodendri flavones and Radix Angelicae Sinensis polysaccharide three are pressed 2:2:1 quality proportioning forms compound.
The compound that embodiment 4, rodgersflower rhizome flavones, Cortex Phellodendri flavones and Radix Angelicae Sinensis polysaccharide form is to pig lymphocyte transformation experiment：
（1）Aseptic collection pig vena cava anterior blood 20mL is put into the anticoagulant tube containing heparin sodium.
（2）Anticoagulation and dilution are pressed 1:1 is diluted, and dilution and lymphocyte separation medium are pressed 1:2 mixings point
It is attached in autoclaved centrifuge tube, horizontal centrifuge 1850r/min, carries out 30min centrifugations.
（3）The buffy coat for drawing middle white floccule, is placed in clean high pressure sterilization centrifuge tube, and cleaning is added
1500r/min after liquid mixing, 10min are centrifuged；Liquid is discarded, the piping and druming of 1mL erythrocyte cracked liquids is added uniformly, stands 3min, adds
Enter 1500r/min after cleaning solution trim, 10min centrifugations；Discard liquid.
（4）Complete medium is prepared：1640 culture mediums:Fetal calf serum:Dual anti-ratio is 89%:10%:1%.
（5）It takes in 450 μ L lymphocytes suspensions to small centrifuge tube, 50 μ L Trypan Blues is added and count.By cell suspension
It is diluted to 2 × 105It is a, it is placed in carbon dioxide incubator and is cultivated.
（6）3 gained compound of embodiment is dissolved with complete medium, is filtered with miillpore filter, is prepared not
With the drug of concentration gradient.
（7）96 orifice plates are taken, are loaded by different pharmaceutical gradient, the repetition that each concentration gradient is 4, control wells set 4
It repeats, per 100 μ L of hole.
（8）Concanavalin A（ConA）Experiment：It is divided into acellular group, adds the 100 μ L of complete medium containing 20% ConA；
There is groups of cells, adds the 100 μ L of cell suspension containing 20% ConA.
（9）Without concanavalin A（ConA）Experiment：It is divided into acellular group, 100 μ L of complete medium is added；There is groups of cells, adds
100 μ L of lymphocyte cell suspension.
（10）It is placed in carbon dioxide incubator and carries out 42 ~ 44h of culture.Be protected from light plus 5% MTT solution per 20 μ L of hole, titanium dioxide
Carbon incubator culture 4h, is protected from light plus dimethyl sulfoxide is per 100 μ L of hole.OD values are measured at ultraviolet specrophotometer 570nm.
（11）It calculates：Conversion ratio=（OD experimental group-OD control groups）/ OD control group × 100%；As a result it is more than 1 and indicates drug
There is immunoenhancement result.
（12）As a result：When drug concentration is 5mg/mL, 2.5mg/mL, 1.25mg/mL, corresponding turn of ConA compounds
Rate is：3.294、3.275、1.908.The result shows that 3 gained compound of embodiment has under the concentration conditions of 1.25 ~ 5mg/mL
The effect for the immunity that is significantly increased.
Embodiment 5 improves rat immunity power test experience：
It tests the 0th day, by the adult healthy cleaning grade Kunming kind male rat that 21 weight are 180-200 g, is randomly divided into 3
Group, i.e.,：Blank control group（Group）, cyclophosphamide group（Group）, rodgersflower rhizome flavones, Cortex Phellodendri flavones and Radix Angelicae Sinensis polysaccharide compound group（Group）, referred to as rock cypress works as group, after first experimental raising 3 days, removesGroup is outer, and remaining 2 groups fed ring phosphinylidyne at the 4th, 5,6 day
Amine.It tests the 8th day, removesGroup,Group is outer,Group progress rock cypress feeds when composition of medicine, 200 mgkg-1。Group,Group is fed
200 mgkg of physiological saline-1。
In experiment, blood was collected within the 0th, 7,14,21,28,35 day, measures routine blood indexes and for statistical analysis respectively.
As a result：During the test, the rat of blank control group is acted normally, and feeding is normal, and mental status is good, does not go out
Existing death.For rock cypress when medicine group is after feeding cyclophosphamide, spirit is depressed, and loss of appetite, after feeding drug, mental status is bright
Aobvious to improve, appetite increases.The cyclophosphamide group for feeding cyclophosphamide normally feeds physiological saline daily without any processing, gives
Identical stressed condition is given, spirit is depressed, and loss of appetite, the later stage occurs dead.
The result shown in Fig. 1-2 as it can be seen that control rock cypress when group, blank control group and cyclophosphamide group 2 immune indexes,
The trend risen after falling before is presented, 0-7d declines, and 7-35d rises.For rock cypress as group 7-14d, ascendant trend is apparent, is more than
Blank control group and cyclophosphamide group.At the 7th day cyclophosphamide group and rock cypress when group it is poorer than blank group after feeding cyclophosphamide
It is different notable（P＜ 0.05）；At the 14th day, after each group feeds relative medicine, rock cypress was higher than cyclophosphamide group significant difference when group
（P＜ 0.05）；At 21 days, rock cypress was when group is than cyclophosphamide group significant difference（P＜ 0.05）；At the 35th day, each group was restored
Normal level.It can be seen that feeding compound after carrying out immunosupress to rat, intravital immune system can be quickly swashed, adjust machine
Body immunity function.
The basic principles, main features and advantages of the present invention have been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by claims and its equivalent
It defines.
Claims (2)
1. a kind of rodgersflower rhizome flavones, the application of Cortex Phellodendri flavones and Radix Angelicae Sinensis polysaccharide compound in preparing veterinary drug, which is characterized in that rodgersflower rhizome
Flavones crude extract, Cortex Phellodendri flavones crude extract and Radix Angelicae Sinensis polysaccharide crude extract press 2:2:1 quality proportioning forms compound, is used to prepare
Enhance the medicated premix in the veterinary drug of animal body immune function, the rodgersflower rhizome flavones crude extract extracts system from rodgersflower rhizome plant
Standby to obtain, the Cortex Phellodendri flavones crude extract is extracted from Calusena lansium tree plant and is prepared, and the Radix Angelicae Sinensis polysaccharide crude extract is from Radix Angelicae Sinensis
Extraction is prepared in plant, and what the rodgersflower rhizome flavones crude extract, Cortex Phellodendri flavones crude extract and Radix Angelicae Sinensis polysaccharide crude extract formed answers
Object is closed as the medicated premix in veterinary drug, the medicated premix additive amount being added in veterinary drug is 1.25 ~ 5mg/mL.
2. rodgersflower rhizome flavones as described in claim 1, the application of Cortex Phellodendri flavones and Radix Angelicae Sinensis polysaccharide compound in preparing veterinary drug,
It is characterized in that, the preparation of the compound of the rodgersflower rhizome flavones crude extract, Cortex Phellodendri flavones crude extract and Radix Angelicae Sinensis polysaccharide crude extract composition
Steps are as follows：
One, the extraction of rodgersflower rhizome flavones：
（1）The rodgersflower rhizome rhizome dried is smashed it through into 40 mesh sieve, obtains rodgersflower rhizome powder；
（2）Rodgersflower rhizome powder is taken, by feed liquid mass ratio 1:（55-65）60% ethyl alcohol of concentration is added to impregnate, stands overnight, ultrasonic wave work(
10-20min is extracted under the conditions of rate 280-320W, 65-75 DEG C of water-bath 2.5-3.5 hours in water-bath；
（3）It is filtered with vacuum pump and removes the dregs of a decoction, then ethyl alcohol is flung to through depressurizing Rotary Evaporators, be put into 65-75 DEG C of thermostatic drying chamber
It is dry, obtain the rodgersflower rhizome flavones crude extract that purity content is 35-40%；
Two, the extraction of Cortex Phellodendri flavones：
（1）The bark for the wampee dried is smashed it through into 40 mesh sieve, obtains Cortex Phellodendri powder；
（2）Phellodendron powder is taken, by feed liquid mass ratio 1:（55-65）60% ethyl alcohol of concentration is added to impregnate, stands overnight, ultrasonic wave work(
10-20min is extracted under the conditions of rate 280-320W, in 65-75 DEG C of water-bath water-bath 2.5-3.5 hours；
（3）It is filtered with vacuum pump and removes the dregs of a decoction, then ethyl alcohol is flung to through depressurizing Rotary Evaporators, be put into thermostatic drying chamber control and exist
It is dry under the conditions of 65-75 DEG C, obtain the Cortex Phellodendri flavones crude extract that content is not less than 12%；
Three, the extraction of Radix Angelicae Sinensis polysaccharide：
（1）It takes the dry root of plant Radix Angelicae Sinensis to smash it through 20 mesh sieve, obtains angelica powder；
（2）Angelica powder is taken, by feed liquid mass ratio 1:（35-45）Distilled water is added, in 55-65 DEG C of water-bath and ultrasonic power 280-
10-20min is extracted under the conditions of 320W；
（3）The liquid that previous step obtains extracts 1h in 75-85 DEG C of hot water, is filtered after cooling and obtains concentrate, in concentrate
The ethyl alcohol of middle addition a concentration of 95%, makes ethanol content in concentrate reach 80%, is stood overnight under 4 DEG C of cold environmental conditions；
（4）The isolated precipitations of 1500r/min and supernatant in centrifuge, collection are dried under the conditions of being deposited in 60 DEG C, are obtained pure
The Radix Angelicae Sinensis polysaccharide that degree is 30%；
Four, gained rodgersflower rhizome flavones, Cortex Phellodendri flavones and Radix Angelicae Sinensis polysaccharide three are pressed 2:2:It is yellow that 1 quality proportioning forms rodgersflower rhizome, Cortex Phellodendri
Ketone and Radix Angelicae Sinensis polysaccharide compound.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810854614.4A CN108635445A (en) | 2018-07-30 | 2018-07-30 | The application of rodgersflower rhizome flavones, Cortex Phellodendri flavones and Radix Angelicae Sinensis polysaccharide compound in preparing veterinary drug |
Applications Claiming Priority (1)
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| CN112076231A (en) * | 2020-09-18 | 2020-12-15 | 云南农业大学 | Compound raw material for preparing veterinary drug for pigs and application of compound raw material |
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| CN112076231B (en) * | 2020-09-18 | 2022-04-12 | 云南农业大学 | Compound raw material for preparing veterinary drug for pigs and application of compound raw material |
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