CN108721357A - Application of the erigeron breviscapus extracts in preparing veterinary drug - Google Patents
Application of the erigeron breviscapus extracts in preparing veterinary drug Download PDFInfo
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- CN108721357A CN108721357A CN201810854619.7A CN201810854619A CN108721357A CN 108721357 A CN108721357 A CN 108721357A CN 201810854619 A CN201810854619 A CN 201810854619A CN 108721357 A CN108721357 A CN 108721357A
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- Prior art keywords
- veterinary drug
- fleabane flower
- erigeron breviscapus
- extracts
- fleabane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
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- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention discloses a kind of application of erigeron breviscapus extracts in preparing veterinary drug, erigeron breviscapus extracts are used to prepare the medicated premix in the veterinary drug of enhancing animal body immune function, the erigeron breviscapus extracts are the fleabane flower flavones extracted from oil lamp flowering plant, and it is 1.25 ~ 5mg/mL that the fleabane flower flavones, which is added to the drug concentration in veterinary drug,；Gained fleabane flower flavones crude extract bioactivity is good, shows there is facilitation to the conversion of lymphocyte in pig lymphocyte transformation experiment；To leucocyte in the low rat blood serum of immunogenicity（WBC）, neutrophil leucocyte（Gran）, lymphocyte（Lym）Content is improved effect, and Immunosuppreessed rats physical condition is made to be restored, and shows that fleabane flower flavones crude extract has the effect of significantly increasing animal body immune function, can be used as the veterinary drug raw material for enhancing animal body immune function.
Description
Technical field
The invention belongs to animal medicine herbal medicine preparing technical field, a kind of specifically flavones crude extract in fleabane flower
Application in preparing veterinary drug.
Background technology
As society continues to develop, consumer gradually increasing to the safety of food and to environmental consciousness, at present we
The animal food eaten remaining veterinary drug problem and veterinary drug be increasingly taken seriously to the pollution problem of natural environment.
Find the safe and effective drug that cultivated animals disease is prevented instead of antibiotic, it appears be of great significance.In recent years, medium-height grass
Medicine is extensive and toxic side effect is relatively low by self-acting, and the characteristics of be not likely to produce drug resistance, in livestock and poultry cultivation industry
Receive public concern.
Fleabane flower is drying herb also known as erigeron breviscapus, the eastern chrysanthemum of feverfew Erigeron breviscapus.Fleabane flower has the solution that is slightly cold
The effect of poison, dispelling wind and eliminating dampness, promoting blood circulation and removing blood stasis, clearing and activating the channels and collaterals, anti-inflammatory analgetic, fleabane flower is mainly used for human body in terms of medicine at present
The treatment of diseases of cardiovascular and cerebrovascular systems develops and uses less in terms of animal medicine.Before the application proposes, fleabane flower crude extract
As the application of additive there is not yet relevant report in the medicine material for preparing veterinary drug.
Contain a large amount of Flavonoid substances in plant fleabane flower, a kind of raw material resources of veterinary drug is can be used as, to the hair of herbal medicine
Exhibition and fleabane flower, which further develop and use, to have very great help.
Cell factor and immunoglobulin that immunocyte generates are the signal of interest and substance of immunocyte interaction
Basis plays a significant role in Cellular Immunity and humoral immunity regulation and control.Immune cell types are more, widely distributed.Blood
In maturation immunity cell be commonly called as leucocyte, be mainly made of mononuclearcell, including monocyte, lymphocyte and grain are thin
Born of the same parents.Lymphocyte includes T, bone-marrow-derived lymphocyte, is most important immunocyte, and T lymphocytes participate in cellular immunity, bone-marrow-derived lymphocyte
Participate in humoral immunity.
Cyclophosphamide is usually used in antitumor treatment, and maximum side effect is exactly immunosupress, is especially exempted to body fluid
The inhibition of epidemic disease, so, cyclophosphamide is usually used in the foundation that animal immune inhibits model.
Invention content
Present invention aims in view of the above-mentioned deficiencies in the prior art, provide flavones crude extract in a kind of fleabane flower
Enhance the application in terms of animal body immune function in preparing veterinary drug, new raw material resources are provided for veterinary drug exploitation.
The present invention is achieved by following technical solution：A kind of application of erigeron breviscapus extracts in preparing veterinary drug, lamp
Small cup flower extract is used to prepare the medicated premix in the veterinary drug of enhancing animal body immune function, and the erigeron breviscapus extracts are
The fleabane flower flavones extracted from oil lamp flowering plant, the fleabane flower flavones be added to the drug concentration in veterinary drug be 1.25 ~
5mg/mL。
The erigeron breviscapus extracts are extracted by following steps and are prepared：
（1）The fleabane flower complete stool dried is smashed it through into 40 mesh sieve, obtains oil lamp pollen；
（2）Oil lamp pollen is taken, by feed liquid mass ratio 1:（55-65）60% ethyl alcohol of concentration is added to impregnate, stands overnight, ultrasonic wave work(
10-20min is extracted under the conditions of rate 280-320W, 65-75 DEG C of water-bath 2.5-3.5 hours in water-bath；
（3）It is filtered with vacuum pump and removes the dregs of a decoction, then ethyl alcohol is flung to through depressurizing Rotary Evaporators, be put into 65-75 DEG C of thermostatic drying chamber
It is dry, obtain the fleabane flower flavones crude extract that purity content is 16-18%, recovery rate 29%.
The fleabane flower flavones crude extract is shown in pig lymphocyte conversion test with raising body's immunity
Effect.
The fleabane flower flavones crude extract embodies enhancing animal body in improving rat immunity power test experience and exempts from
The effect of epidemic disease function.
One of the purposes of the fleabane flower flavones crude extract is as a kind of veterinary drug raw material resources, for enhancing animal machine
Body immunity function.
The beneficial effects of the invention are as follows：
1. fleabane flower crude flavonoid powder extraction process is simple, extraction conditions are mild, gained fleabane flower crude flavonoid powder activity is strong.
2. it is aobvious that pig lymphocyte transformation experiment and rat immunity power test experience show that the fleabane flower crude flavonoid powder has
The effect of enhancing animal body immune function is write, exploitation is safe and convenient to use at veterinary drug, expands the purposes of effect of Erigeron.
The advantages that 3. fleabane flower crude flavonoid powder has material source wide, significant in efficacy, pharmaceutical safety.
Description of the drawings
Fig. 1 is influence schematic diagram of the fleabane flower flavones crude extract to rat WBC.
Fig. 2 is influence schematic diagram of the fleabane flower flavones crude extract to rat Gran.
Fig. 3 is influence schematic diagram of the fleabane flower flavones crude extract to rat Lym.
Specific implementation mode
With reference to embodiment, the present invention is described in further detail.
It will be understood to those of skill in the art that the following example is merely to illustrate the present invention, and it should not be regarded as limiting this hair
Bright range.In the examples where no specific technique or condition is specified, according to technology or condition described in document in the art
Or it is carried out according to product description.Production firm person is not specified in material therefor or equipment, is that can be obtained by buying
Conventional products.
Embodiment 1：The preparation of fleabane flower flavones crude extract.
（1）The fleabane flower complete stool dried is crushed, oil lamp pollen is obtained；
（2）10g oil lamp pollen is taken, the ethyl alcohol that 600mL contents are 60% is added and impregnates, stands overnight, ultrasonic 15min, 70 DEG C of water-baths
Pot water-bath 3 hours；
（3）It is filtered with vacuum pump and removes the dregs of a decoction, then ethyl alcohol is flung to through depressurizing Rotary Evaporators, it is dry to be put into 70 DEG C of thermostatic drying chambers
Dry, it is 17.738% fleabane flower flavones to obtain 2.9g contents.
Embodiment 2：Fleabane flower flavones crude extract is to pig lymphocyte transformation experiment.
（1）Aseptic collection pig vena cava anterior blood 20mL is put into the anticoagulant tube containing heparin sodium.
（2）Anticoagulation and dilution are pressed 1:1 mass ratio is diluted, and dilution and lymphocyte separation medium are pressed 1:2
Mass ratio mixing be dispensed into autoclaved centrifuge tube, horizontal centrifuge 1850r/min, 30min are centrifuged.
（3）The buffy coat for drawing middle white floccule, is placed in clean high pressure sterilization centrifuge tube, and cleaning is added
1500r/min after liquid mixing, 10min are centrifuged；Liquid is discarded, the piping and druming of 1mL erythrocyte cracked liquids is added uniformly, stands 3min, adds
Enter 1500r/min after cleaning solution trim, 10min centrifugations；Discard liquid.
（4）Complete medium is prepared：1640 culture mediums:Fetal calf serum:Dual anti-ratio is 89%:10%:1%.
（5）It takes in 450 μ L lymphocytes suspensions to small centrifuge tube, 50 μ L Trypan Blues is added and count.By cell suspension
It is diluted to 2 × 105It is a, it is placed in carbon dioxide incubator and is cultivated.
（6）1 gained fleabane flower flavones crude extract of embodiment is dissolved with complete medium, was carried out with miillpore filter
The drug of various concentration gradient is prepared in filter.
（7）96 orifice plates are taken, are loaded by different pharmaceutical gradient, the repetition that each concentration gradient is 4, control wells set 4
It repeats, per 100 μ L of hole.
（8）Concanavalin A（ConA）Experiment：It is divided into acellular group, adds the 100 μ L of complete medium containing 20% ConA；
There is groups of cells, adds the 100 μ L of cell suspension containing 20% ConA.
（9）Without concanavalin A（ConA）Experiment：It is divided into acellular group, 100 μ L of complete medium is added；There is groups of cells, adds
100 μ L of lymphocyte cell suspension.
（10）It is placed in carbon dioxide incubator and carries out 42 ~ 44h of culture.Be protected from light plus 5% MTT solution per 20 μ L of hole, titanium dioxide
Carbon incubator culture 4h, is protected from light plus dimethyl sulfoxide is per 100 μ L of hole.OD values are measured at ultraviolet specrophotometer 570nm.
（11）It calculates：Conversion ratio=（OD experimental group-OD control groups）/ OD control group × 100%；As a result it is more than 1 and indicates drug
There is immunoenhancement result.
（12）As a result：When drug concentration is 5mg/mL, 2.5mg/mL, 1.25mg/mL, ConA fleabane flower flavones slightly carries
The corresponding conversion ratio of object is：1.607,1.407,1.022.The result shows that 1 gained fleabane flower flavones crude extract of embodiment 1.25 ~
The effect for significantly improving immunity of organisms is embodied under the concentration conditions of 5mg/mL.
Embodiment 3：Improve rat immunity power test experience.
It tests the 0th day, 21 Laboratory Rats is divided into fleabane flower group, cyclophosphamide-a control group, blank control group, each
Each 7 of group, acquires every group of mouse blood respectively, carries out leucocyte（WBC）, neutrophil leucocyte（Gran）, lymphocyte（Lym）It surveys
After fixed, in addition to blank control, cyclophosphamide 50mg/kg injected, 1 times/day, is used in conjunction 3 days.Acquisition sample measures respectively within 7th day
Each group WBC, Gran, Lym index, same day fleabane flower group gavage fleabane flower flavones crude extract 200mg/kg after sampling, other groups fill
Stomach normal saline, is used in conjunction 28 days, as a result as shown in Figs. 1-3, the variation feelings of WBC, Gran and Lym is shown respectively by 1 times/day
Condition.
From the above results, it can be seen that 3 immune indexes of fleabane flower group and cyclophosphamide-a control group present and rise after falling before
Trend declines for 0-7 days, rises within 7-35 days.Fleabane flower group the 7-14 days, ascendant trend is apparent, be more than blank control group, 0 day with
Comparing difference is notable within 7th day（p<0.05）, the 7th day with the 14th day it is poor it is heteropolar significantly (p<0.01).Drug cyclophosphamide pair
The trend gradually risen is presented within the 7-35 days according to group, is not above blank control group, 0 day notable with the 7th day comparing difference（p<
0.05）.Index to the 28th day each group tends to blank control group, and body has restored normal.It can be seen that fleabane flower flavones crude extract
Compound can quickly swash intravital immune system after rat oral gavage immunosuppressor, adjust body's immunity.
The basic principles, main features and advantages of the present invention have been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by claims and its equivalent
It defines.
Claims (2)
1. a kind of application of erigeron breviscapus extracts in preparing veterinary drug, which is characterized in that erigeron breviscapus extracts are used to prepare enhancing
Medicated premix in the veterinary drug of animal body immune function, the erigeron breviscapus extracts are the lamp extracted from oil lamp flowering plant
Small cup flower flavones, the fleabane flower flavones are used for a concentration of 1.25 ~ 5mg/mL of medicated premix in veterinary drug.
2. application of the erigeron breviscapus extracts as described in claim 1 in preparing veterinary drug, which is characterized in that the fleabane flower carries
Take object preparation process as follows：
（1）The fleabane flower complete stool dried is smashed it through into 40 mesh sieve, obtains oil lamp pollen；
（2）Oil lamp pollen is taken, by feed liquid mass ratio 1:（55-65）60% ethyl alcohol of concentration is added to impregnate, stands overnight, ultrasonic wave work(
10-20min is extracted under the conditions of rate 280-320W, 65-75 DEG C of water-bath 2.5-3.5 hours in water-bath；
（3）It is filtered with vacuum pump and removes the dregs of a decoction, then ethyl alcohol is flung to through depressurizing Rotary Evaporators, be put into 65-75 DEG C of thermostatic drying chamber
It is dry, obtain the fleabane flower flavones crude extract that purity content is 16-18%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810854619.7A CN108721357A (en) | 2018-07-30 | 2018-07-30 | Application of the erigeron breviscapus extracts in preparing veterinary drug |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810854619.7A CN108721357A (en) | 2018-07-30 | 2018-07-30 | Application of the erigeron breviscapus extracts in preparing veterinary drug |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108721357A true CN108721357A (en) | 2018-11-02 |
Family
ID=63941898
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810854619.7A Pending CN108721357A (en) | 2018-07-30 | 2018-07-30 | Application of the erigeron breviscapus extracts in preparing veterinary drug |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108721357A (en) |
-
2018
- 2018-07-30 CN CN201810854619.7A patent/CN108721357A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 吴先闯等: "TGF-β1抑制剂灯盏花素对肿瘤诱导的小鼠免疫抑制的逆转作用", 《河南大学学报（医学版）》 * |
| 张俭等: "灯盏花黄酮超声波辅助提取工艺研究", 《江苏农业科学》 * |
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Application publication date: 20181102 |
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