CN104721620A - Leafy composition for enhancing immunity - Google Patents
Leafy composition for enhancing immunity Download PDFInfo
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- CN104721620A CN104721620A CN201510119173.XA CN201510119173A CN104721620A CN 104721620 A CN104721620 A CN 104721620A CN 201510119173 A CN201510119173 A CN 201510119173A CN 104721620 A CN104721620 A CN 104721620A
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- folium
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- granule
- enhancing immunity
- herba lophatheri
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Abstract
The invention provides a leafy composition for enhancing immunity and a preparation method and an application thereof. The composition is prepared from ashitaba, ginseng leaf, folium cortex eucommiae and lophatherum gracile. The composition provided by the invention is small in dosage and is only taken once every day, so that the application is convenient.
Description
Technical field
The present invention relates to a kind of composition and method of making the same and the application with enhancing body immunity, belong to medical art.
Background technology
Improve immunity of organisms and need aggregate measures, comprise all many-sides such as exercise, diet, sleep, prophylactic immunization, immunomodulator, chemoprophylaxis and treatment means.But with the quickening pace of modern life, health product are more and more subject to people's welcome.But existing health product taking dose is large, every day takes that frequency is high, mouthfeel is poor, cost is high, limits the use crowd of health product.
Summary of the invention
The object of this invention is to provide a kind of composition and method of making the same and the application with enhancing immunity.
For foregoing invention object, the invention provides following technical scheme:
The Chinese medicine composition for the treatment of primary dysmenorrhea of the present invention, is characterized in that: it is made with the medical material of following weight portion: angelica keiskei koidzumi 1 ~ 5 part, Folium Ginseng 1 ~ 5 part, Folium Eucommiae 1 ~ 5 part, Herba Lophatheri 1 ~ 5 part.
Chinese medicine composition of the present invention is preferably made with the raw material of following weight portion: angelica keiskei koidzumi 1 ~ 2 part, Folium Ginseng 1 ~ 2 part, Folium Eucommiae 1 ~ 2 part, Herba Lophatheri 1 ~ 2 part.
Above-mentioned Chinese medicine composition of the present invention adds various customary adjuvant required when preparing different dosage form, such as diluent, adhesive, disintegrating agent, fluidizer, lubricant, correctives, enclose material, adsorbing material etc. are prepared into the conventional oral formulations of any one with the method for Chinese medicinal of routine, such as, can be granule, powder, tablet, capsule, pill, oral liquid, drop pill etc.
Oral formulations of the present invention is preferably granule, and granule is prepared according to the following step: take angelica keiskei koidzumi, Folium Ginseng, Folium Eucommiae and Herba Lophatheri, extracting in water, extracting solution is condensed into thick paste, adds pharmaceutic adjuvant and makes granule.
Chinese medicine composition of the present invention is for the preparation of the application of the health product of enhancing immunity.
Beneficial effect of the present invention is mainly:
(1) there is after compositions compatibility of the present invention the enhancing immunity effect of highly significant.
(2) compositions of the present invention is made up of angelica keiskei koidzumi, Folium Ginseng, Folium Eucommiae and Herba Lophatheri, and raw material sources are extensive, and cost is low, can provide inexpensive health product for low immunity crowd.
(3) angelica keiskei koidzumi, Folium Ginseng, Folium Eucommiae and Herba Lophatheri own taste are better, do not need to add correctives and namely have good mouthfeel.
(4) not only dosage is little for compositions of the present invention, and every day only need take 1 time, easy to use.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, but should note scope of the present invention not by any restriction of these examples.
Embodiment 1
Angelica keiskei koidzumi 10g, Folium Ginseng 10g, Folium Eucommiae 10g, Herba Lophatheri 10g
Above-mentioned medical material is added 20 times amount water soaking 1 hour, decoct 2 times, each 1 hour, merge 2 decocting liquids, centrifugal filtration, filtrate is condensed into thick paste, is mixed by thick paste with dextrin, makes granule, and dry, mix homogeneously, incapsulates, and obtains capsule.Every capsules 200mg, takes 1 every day, each 1.。
Embodiment 2
Angelica keiskei koidzumi 2g, Folium Ginseng 10g, Folium Eucommiae 10g, Herba Lophatheri 10g
Above-mentioned medical material is added 10 times amount water soaking 1 hour, decoct 2 times, each 1 hour, merge 2 decocting liquids, centrifugal filtration, filtrate is condensed into thick paste, is mixed by thick paste with dextrin, makes granule, and dry, mix homogeneously, incapsulates, and obtains capsule.Every capsules 200mg, takes 1 every day, each 1.
Embodiment 3
Angelica keiskei koidzumi 5g, Folium Ginseng 10g, Folium Eucommiae 10g, Herba Lophatheri 5g
Above-mentioned medical material is added 10 times amount water soaking 1 hour, decoct 2 times, each 1 hour, merge 2 decocting liquids, centrifugal filtration, filtrate is condensed into thick paste, is mixed by thick paste with dextrin, makes granule, drying, mixs homogeneously granule with Pulvis Talci, magnesium stearate, and tabletting, obtains tablet.Every sheet 300mg, takes 1 every day, each 1.
Embodiment 4
Angelica keiskei koidzumi 10g, Folium Ginseng 10g, Folium Eucommiae 3g, Herba Lophatheri 3g
Above-mentioned medical material is added 10 times amount water soaking 1 hour, decoct 2 times, each 1 hour, merge 2 decocting liquids, centrifugal filtration, filtrate is condensed into thick paste, and mix homogeneously with the Macrogol 4000 melted, dripping becomes drop pill, obtains drop pill.Every ball 50mg, takes 1 every day, each 8.
Embodiment 5
Angelica keiskei koidzumi 10g, Folium Ginseng 10g, Folium Eucommiae 5g, Herba Lophatheri 5g
Above-mentioned medical material is added 10 times amount water soaking 1 hour, decoct 2 times, each 1 hour, merge 2 decocting liquids, centrifugal filtration, concentrated, add ethanol and reach 60% to ethanol content, precipitate with ethanol 48 hours, get supernatant and reclaim ethanol, add suitable quantity of water, activated carbon decolorizing, filters, and divides and is filled in oral liquid bottle, sterilizing, obtains oral liquid.Often prop up oral liquid 10ml, take 1 every day, each 1.
Embodiment 6
Angelica keiskei koidzumi 5g, Folium Ginseng 5g, Folium Eucommiae 5g, Herba Lophatheri 5g
Above-mentioned medical material is added 10 times amount water soaking 1 hour, decoct 2 times, each 1 hour, merge 2 decocting liquids, centrifugal filtration, concentrated, add ethanol and reach 60% to ethanol content, precipitate with ethanol 48 hours, get supernatant and reclaim ethanol, add suitable quantity of water, activated carbon decolorizing, filters, and divides and is filled in oral liquid bottle, sterilizing, obtains oral liquid.Often prop up oral liquid 10ml, take 1 every day, each 1.
Embodiment 7
Angelica keiskei koidzumi 100g, Folium Ginseng 100g, Folium Eucommiae 100g, Herba Lophatheri 100g
Above-mentioned medical material is added 20 times amount water soaking 1 hour, decoct 2 times, each 1 hour, merge 2 decocting liquids, centrifugal filtration, filtrate is condensed into thick paste, is mixed by thick paste with appropriate dextrin and Icing Sugar, make granule, drying, namely obtained granule 100g, every g are equivalent to containing crude drug in whole 4g, be distributed into 50 bags, often wrap 2g.
Usage and consumption: oral, take 1 every day, each 2g.
Embodiment 8 enhancing immunity is tested
(1) laboratory animal
Cleaning grade Male Kunming strain mice 200, quality 18 ~ 21g, is provided by Shanghai Slac Experimental Animal Co., Ltd., raises in the environment of temperature 18 ~ 24 DEG C, humidity 40% ~ 70%.
(2) medicine: positive control medicine Ganoderma spore powder with cellular wall broken, purchased from Beijing Tongrentang.Medicine is the present composition (preparing granule by embodiment 7), and every g is equivalent to containing crude drug in whole 4g.
(3) animal grouping and medication
Animal is divided into 4 large groups, and every large group 50 mices are divided into negative control group, positive controls and high, medium and low dose drug group at random.Immunization experiment I group measures Tardive allergy; Immunization experiment II group measures carbonic clearance ability; Immunization experiment III group measures Function of lymphocyte transform; Immunization experiment IV group measures macrophage phagocytic effect.Negative control group gives purified water; Positive controls gives Ganoderma spore powder with cellular wall broken, and dosage is 1000mg/Kg; High, medium and low pharmaceutical composition amount is respectively 297mg/Kg, 99mg/Kg, 33mg/Kg, 9,3,1 times that are equivalent to that people intends quantity according to the weight.Each group of continuous gastric infusion of mouse 15 days.
(4) experimental technique and result
1. Tardive allergy
After the administration of mice last, every mouse web portion unhairing, scope is about 3cm × 3cm, and by acetone sesame oil solution (1:1, V/V) the 50 μ l uniform application sensitization containing 1%DNCB, strengthens 1 next day; The acetone sesame oil solution of equivalent without DNCB is smeared after normal group mice unhairing.Sensitization 7 days, attacked on two sides inside and outside each group of mouse right ear by 1%DNCB acetone sesame oil solution 10 μ l uniform application, left ear smears the acetone sesame oil solution of equivalent without DNCB.24h after attacking, cervical dislocation puts to death mice, cuts left and right auricular concha, weighs, and calculates the difference of left and right ear weight.Following result is obtained after date processing:
| Project | Negative control group | Positive controls | Low dose group | Middle dosage group | High dose group |
| The difference (mg) of left and right ear weight | 2.15±1.02 | 3.82±1.03* | 5.07±1.05* | 5.62±1.02* | 6.52±1.03* |
* p<0.05 compared with negative control group is represented
2. carbonic clearance ability
After mice last administration 30min, tail iv india ink (0.1ml/10g).Add normal saline 4ml by the every 1ml of india ink before injection to dilute.Prepared Chinese ink is once injecting timing immediately.Blood 20 μ l is got from animal angular vein clump respectively at 2,10min after injection.The blood obtained is added in 2ml 0.1% sodium carbonate liquor and shake up.Absorbance (OD) value is measured by microplate reader at 650nm wavelength place after about 2h.Index (K) is cleaned up: K=(logOD by following formulae discovery
1-logOD
2)/(T
10-T
2).In formula, OD
1for T
1oD value time (animal injection india ink 2min); OD
2for T
2oD value time (animal injection india ink 10min).Engulf coefficient a=K × 1/3 × body weight/(liver weight+spleen weight).Following result is obtained after date processing:
| Project | Negative control group | Positive controls | Low dose group | Middle dosage group | High dose group |
| Engulf coefficient | 10.63±1.15 | 13.57±1.09* | 15.82±1.08* | 16.95±1.06* | 17.72±1.03* |
* p<0.05 compared with negative control group is represented
3. Function of lymphocyte transform
After the administration of mice last, cervical dislocation is put to death, asepticly get spleen, be placed in the plate filling appropriate aseptic Hank ' s liquid, gently spleen ground, make single cell suspension, 200 eye mesh screens filter, wash 2 times with Hank ' s liquid, at every turn centrifugal 10 minutes living cell counting numbers (should more than 95%), adjustment cell concentration is 3 × 106/ml.A cell suspension point holes is added in 24 well culture plates, every hole 1ml, 75 μ l ConA (7.5 μ g/ml).Put 37 DEG C, cultivate 72 hours in 5%CO2 incubator.Cultivation terminates first 4 hours, and every hole sucks supernatant 0.7ml gently, adds 0.7ml not containing the RPMI RPMI-1640 of calf serum, adds MTT (5mg/ml) 50 μ l hole simultaneously, continue cultivation 4 hours.Cultivate and terminate, every hole adds 1ml acid isopropyl alcohol, and piping and druming mixing, makes purple crystal dissolve completely.Lysate is moved in 2ml cuvette, measure optical density value (OD) at 570nm wavelength place.Relatively add the difference with the absorbance not adding Con A, take statistics process, obtains following result after date processing:
| Project | Negative control group | Positive controls | Low dose group | Middle dosage group | High dose group |
| The difference of absorbance | 0.14±0.23 | 0.18±0.11* | 0.22±0.21* | 0.25±0.11* | 0.26±0.12* |
* p<0.05 compared with negative control group is represented
4. macrophage phagocytic effect
Fresh cock blood, microscope slide, quick Ji's nurse Sa dye liquor.
The preparation of A Shi liquid: accurately take glucose 2.05g, sodium citrate 0.80g, citric acid 0.05g, sodium chloride 0.42g, dissolve with distilled water and be settled to 1000ml.
The preparation of chicken red blood cells suspension: select healthy sheep, is placed with the aseptic conical flask of bead from blood sample, covers bottle stopper, shakes towards same direction with hands, through fully mixing until grumeleuse is fully formed, removes fibrin.Blood sample is moved in the conical flask containing A Shi liquid, shakes up gently, put 4 DEG C of refrigerator storages, for subsequent use.Get appropriate anticoagulant Sanguis Gallus domesticus physiological saline solution before using and wash 3 times, the centrifugal 10min of each 2000rpm, be made into cell suspension (having can not using of haemolysis) with physiological saline solution according to volume ratio 1:50.
After the administration of mice last, every Mus lumbar injection 20% chicken erythrocyte suspension 1ml, interval 30min cervical dislocation is put to death, be fixed on Mus plate, cut off abdominal skin, injecting normal saline 2ml, rotate Mus plate 1min, sucking-off abdominal cavity washing liquid 1ml divides and drips on 2 slides, 37 DEG C of incubator 30min, with normal saline rinsing, dry, fix with acetone+methanol (1+1), 4%Giemsa-phosphate buffer dyeing 3min, then dry with distilled water rinsing, with oily mirror microscopy, calculate phagocytic index.Following result is obtained after date processing:
| Project | Negative control group | Positive controls | Low dose group | Middle dosage group | High dose group |
| Engulf coefficient | 0.15±0.21 | 0.19±0.12* | 0.23±0.11* | 0.26±0.01* | 0.28±0.12* |
(5) interpretation
As can be seen from the above results, present composition low dose group, when oral dose is only a thirtieth of positive control medicine Ganoderma spore powder with cellular wall broken, just shows better immunity reinforced effects.
Claims (5)
1. there is a compositions for enhancing immunity, it is characterized in that: it is made with the medical material of following weight portion: angelica keiskei koidzumi 1 ~ 5 part, Folium Ginseng 1 ~ 5 part, Folium Eucommiae 1 ~ 5 part, Herba Lophatheri 1 ~ 5 part.
2. compositions as claimed in claim 1, is characterized in that being made up of the medical material of following weight portion: angelica keiskei koidzumi 1 ~ 2 part, Folium Ginseng 1 ~ 2 part, Folium Eucommiae 1 ~ 2 part, Herba Lophatheri 1 ~ 2 part.
3. the preparation method of Chinese medicine composition according to claim 1, is characterized in that: method of Chinese medicinal is conveniently prepared into the conventional oral formulations of any one.
4. according to the preparation method of Chinese medicine composition according to claim 3, it is characterized in that, described oral formulations is granule, and granule is prepared according to the following step:
Take angelica keiskei koidzumi, Folium Ginseng, Folium Eucommiae and Herba Lophatheri, extracting in water, extracting solution is condensed into thick paste, adds pharmaceutic adjuvant and makes granule.
5. Chinese medicine composition according to claim 1 is for the preparation of the application of the health product of enhancing immunity.
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| CN201510119173.XA CN104721620A (en) | 2015-03-18 | 2015-03-18 | Leafy composition for enhancing immunity |
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|---|---|---|---|
| CN201510119173.XA CN104721620A (en) | 2015-03-18 | 2015-03-18 | Leafy composition for enhancing immunity |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105764346A (en) * | 2013-11-26 | 2016-07-13 | Wm.雷格利 Jr.公司 | Deposited chewing gum product |
| CN106421462A (en) * | 2016-10-11 | 2017-02-22 | 江苏省中医药研究院 | Traditional Chinese medicine composition with face beautifying function |
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| CN102920806A (en) * | 2012-08-08 | 2013-02-13 | 山西康禾农业有限责任公司 | Nerve-soothing blood-pressure-reducing traditional Chinese medicine tea |
| CN104116076A (en) * | 2014-07-14 | 2014-10-29 | 烟台恒迪克能源科技有限公司 | Angelica keiskei capsule and preparing method thereof |
| CN104208461A (en) * | 2014-08-18 | 2014-12-17 | 河南绿生堂金银花生物发展股份有限公司 | Traditional Chinese medicine composition, and application and preparation method thereof |
| CN104306934A (en) * | 2014-10-17 | 2015-01-28 | 浙江施强制药有限公司 | Medicine composition for enhancing immunity and preparation method thereof |
| CN104383287A (en) * | 2014-12-08 | 2015-03-04 | 苏州普罗达生物科技有限公司 | Composition for increasing immunity |
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2015
- 2015-03-18 CN CN201510119173.XA patent/CN104721620A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102920806A (en) * | 2012-08-08 | 2013-02-13 | 山西康禾农业有限责任公司 | Nerve-soothing blood-pressure-reducing traditional Chinese medicine tea |
| CN104116076A (en) * | 2014-07-14 | 2014-10-29 | 烟台恒迪克能源科技有限公司 | Angelica keiskei capsule and preparing method thereof |
| CN104208461A (en) * | 2014-08-18 | 2014-12-17 | 河南绿生堂金银花生物发展股份有限公司 | Traditional Chinese medicine composition, and application and preparation method thereof |
| CN104306934A (en) * | 2014-10-17 | 2015-01-28 | 浙江施强制药有限公司 | Medicine composition for enhancing immunity and preparation method thereof |
| CN104383287A (en) * | 2014-12-08 | 2015-03-04 | 苏州普罗达生物科技有限公司 | Composition for increasing immunity |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105764346A (en) * | 2013-11-26 | 2016-07-13 | Wm.雷格利 Jr.公司 | Deposited chewing gum product |
| CN106421462A (en) * | 2016-10-11 | 2017-02-22 | 江苏省中医药研究院 | Traditional Chinese medicine composition with face beautifying function |
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Application publication date: 20150624 |
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