CN108619236A - Application of the skullcapflavone crude extract in preparing veterinary drug - Google Patents
Application of the skullcapflavone crude extract in preparing veterinary drug Download PDFInfo
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- CN108619236A CN108619236A CN201810854617.8A CN201810854617A CN108619236A CN 108619236 A CN108619236 A CN 108619236A CN 201810854617 A CN201810854617 A CN 201810854617A CN 108619236 A CN108619236 A CN 108619236A
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- skullcapflavone
- crude extract
- veterinary drug
- radix scutellariae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- Natural Medicines & Medicinal Plants (AREA)
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Abstract
The present invention discloses a kind of application of skullcapflavone crude extract in preparing veterinary drug, skullcapflavone crude extract is used to enhance the medicated premix in the veterinary drug of animal body immune function, the skullcapflavone crude extract is extracted from scullcap plant to be obtained, drug concentration of the skullcapflavone crude extract in veterinary drug is 1.25 ~ 5mg/mL, gained skullcapflavone crude extract bioactivity is good, display has facilitation to the conversion of lymphocyte in pig lymphocyte transformation experiment, to leucocyte in the low rat blood serum of immunogenicity（WBC）, neutrophil leucocyte（Gran）, lymphocyte（Lym）Content is improved effect, and Immunosuppreessed rats physical condition is made to be restored, and shows that skullcapflavone crude extract has the effect of significantly increasing animal body immune function, can be used as a kind of veterinary drug raw material of enhancing animal body immune function.
Description
Technical field
The invention belongs to animal medicine herbal medicines to formulate field, and specifically a kind of skullcapflavone crude extract is preparing beast
Application in medicine.
Background technology
As society continues to develop, consumer gradually increasing to the safety of food and to environmental consciousness, at present we
The animal food eaten remaining veterinary drug problem and veterinary drug be increasingly taken seriously to the pollution problem of natural environment.
Find the safe and effective drug that cultivated animals disease is prevented instead of antibiotic, it appears be of great significance.In recent years, medium-height grass
Medicine is extensive and toxic side effect is relatively low by self-acting, and the characteristics of be not likely to produce drug resistance, in livestock and poultry cultivation industry
Receive public concern.
Radix scutellariae be labiate radix scutellariae dry root, be conventional Chinese medicine, containing polysaccharide, flavones, volatile oil etc. it is a variety of at
Point, have it is anti-oxidant, remove free radical, be anti-inflammatory, antitumor, prevent calcium channel, inhibit aldose reductase, antiviral, anti-
The multiple pharmacological effects such as allergy have protective effect to systems such as immune, cardiovascular and cerebrovascular, digestion, nerves.It is main on tcm clinical practice
It is used for the illnesss such as damp-warm syndrome, heat temperature chest tightness and emesis, damp and hot feeling of fullness, rush down dysentery, jaundice, cough with lung heat, hyperpyrexia polydipsia, carbuncle sore tumefacting virus.
Application of the radix scutellariae in treatment of animals at present is less.
Flavone compound is a kind of Secondary Metabolite Production in Plants with 2- phenyl chromone structures, is that one kind is deposited extensively
The active material being in plant medically has the function of anti-oxidant, antitumor, cardiovascular disease resistant and anti-inflammatory etc., tool
Have broad application prospects.Application of the Flavonoid substances in animal productiong at present is less.
Contain a large amount of Flavonoid substances in plant radix scutellariae, can be used as a kind of raw material resources of veterinary drug, the development to herbal medicine
It further develops and uses and has very great help with radix scutellariae.
Cell factor and immunoglobulin that immunocyte generates are the signal of interest and substance of immunocyte interaction
Basis plays a significant role in Cellular Immunity and humoral immunity regulation and control.Immune cell types are more, widely distributed.Blood
In maturation immunity cell be commonly called as leucocyte, be mainly made of mononuclearcell, including monocyte, lymphocyte and grain are thin
Born of the same parents.Lymphocyte includes T, bone-marrow-derived lymphocyte, is most important immunocyte, and T lymphocytes participate in cellular immunity, bone-marrow-derived lymphocyte
Participate in humoral immunity.
Cyclophosphamide is usually used in antitumor treatment, and maximum side effect is exactly immunosupress, is especially exempted to body fluid
The inhibition of epidemic disease, so, cyclophosphamide is usually used in the foundation that animal immune inhibits model.
Invention content：
Present invention aims at a kind of flavones crude extracts of radix scutellariae of offer to enhance animal body immune function side in preparing veterinary drug
The application in face provides new raw material resources for veterinary drug exploitation.
The present invention is achieved by following technical solution：A kind of application of skullcapflavone crude extract in preparing veterinary drug,
The skullcapflavone crude extract is used to enhance the veterinary drug raw material of animal body immune function, and the skullcapflavone crude extract is from radix scutellariae
Extraction obtains in plant, and the drug concentration being added in veterinary drug is 1.25 ~ 2.5mg/mL.
Wherein, the skullcapflavone crude extract is made by the steps：
（1）The radix scutellariae rhizome dried is smashed it through into 40 mesh sieve, obtains radix scutellariae powder；
（2）Radix scutellariae powder is taken, by solid-liquid ratio 1:60 add 60% ethyl alcohol to impregnate, and stand overnight, ultrasonic 15min, 70 DEG C of water-bath water-baths 3
Hour；
（3）It is filtered with vacuum pump and removes the dregs of a decoction, then ethyl alcohol is flung to through depressurizing Rotary Evaporators, put 70 DEG C of thermostatic drying chamber dryings,
Obtain the skullcapflavone crude extract that purity is 2.6%, recovery rate 48%.
In said program, the skullcapflavone crude extract is shown in pig lymphocyte conversion test exempts from raising body
The effect of epidemic disease power.
In said program, the skullcapflavone crude extract embodies enhancing animal in improving rat immunity power test experience
The effect of body's immunity.
In said program, one of the purposes of the skullcapflavone crude extract is as a kind of herbal medicine raw material, for enhancing
Animal body immune function.
The beneficial effects of the invention are as follows：
1. the extraction process of flavones crude extract is simple in radix scutellariae, extraction conditions are mild, and the activity of gained flavones crude extract is strong.
2. pig lymphocyte transformation experiment and raising rat immunity power test experience show the skullcapflavone crude extract
Have the effect of significantly increasing animal body immune function, exploitation is safe and convenient to use at veterinary drug, expands the purposes of baikal skullcap root.
3. skullcapflavone crude extract has many advantages, such as that raw material sources are wide, improves immunity significantly, pharmaceutical safety, green.
Description of the drawings
Fig. 1 is influence of the scutellaria flavones crude extract to rat WBC.
Fig. 2 is influence of the scutellaria flavones crude extract to rat Gran.
Fig. 3 is influence of the skullcapflavone crude extract to rat Lym.
Specific implementation mode
With reference to embodiment, the present invention is described in further detail.
It will be understood to those of skill in the art that the following example is merely to illustrate the present invention, and it should not be regarded as limiting this hair
Bright range.In the examples where no specific technique or condition is specified, according to technology or condition described in document in the art
Or it is carried out according to product description.Production firm person is not specified in material therefor or equipment, is that can be obtained by buying
Conventional products.
" drug " and skullcapflavone crude extract in embodiment are same meaning.
Embodiment 1
（1）The radix scutellariae rhizome dried is smashed it through into 40 mesh sieve, obtains radix scutellariae powder；
（2）10g radix scutellariae powder is taken, the ethyl alcohol that 600mL contents are 60% is added and impregnates, stands overnight, ultrasonic 15min, 70 DEG C of water-baths
Water-bath 3 hours；
（3）It is filtered with vacuum pump and removes the dregs of a decoction, then ethyl alcohol is flung to through depressurizing Rotary Evaporators, put 70 DEG C of thermostatic drying chamber dryings,
Obtain the skullcapflavone crude extract that 4.8g purity contents are 26%.
Embodiment 2
Skullcapflavone crude extract is to pig lymphocyte transformation experiment
（1）Aseptic collection pig vena cava anterior blood 20mL is put into the anticoagulant tube containing heparin sodium；
（2）Anticoagulation and dilution are pressed 1:1 is diluted, and dilution and lymphocyte separation medium are pressed 1:2 ratio mixing
It is dispensed into autoclaved centrifuge tube, horizontal centrifuge 1850r/min, 30min is centrifuged；
（3）The buffy coat for drawing middle white floccule, is placed in clean high pressure sterilization centrifuge tube, and it is mixed that cleaning solution is added
1500r/min after even, 10min centrifugation；Liquid is discarded, the piping and druming of 1mL erythrocyte cracked liquids is added uniformly, stands 3min, is added clear
1500r/min after washing lotion trim, 10min are centrifuged；Discard liquid；
（4）Complete medium is prepared：1640 culture mediums:Fetal calf serum:Dual anti-ratio is 89%:10%:1%；
（5）It takes in 450 μ L lymphocytes suspensions to small centrifuge tube, 50 μ L Trypan Blues is added and count, cell suspension is diluted
To 2 × 105It is a, it is placed in carbon dioxide incubator and is cultivated；
（6）1 gained skullcapflavone crude extract of embodiment is dissolved with complete medium, is filtered, is matched with miillpore filter
The drug of various concentration gradient processed；
（7）96 orifice plates are taken, are loaded by different pharmaceutical gradient, the repetition that each concentration gradient is 4, control wells set 4 repetitions,
Per 100 μ L of hole；
（8）Concanavalin A（ConA）Experiment：It is divided into acellular group, adds the 100 μ L of complete medium containing 20% ConA；Have thin
Born of the same parents' group adds the 100 μ L of cell suspension containing 20% ConA；
（9）Without concanavalin A（ConA）Experiment：It is divided into acellular group, 100 μ L of complete medium is added；There is groups of cells, adds lymph
100 μ L of cell suspension；
（10）It is placed in carbon dioxide incubator and carries out culture 43h.Be protected from light plus 5% MTT solution per 20 μ L of hole, carbon dioxide culture
Case culture 4h, is protected from light plus dimethyl sulfoxide is per 100 μ L of hole.OD values are measured at ultraviolet specrophotometer 570nm；
（11）It calculates：Conversion ratio=（OD experimental group-OD control groups）/ OD control group × 100%；As a result have more than 1 expression drug and exempt from
Epidemic disease enhancing effect.
（12）As a result：When drug concentration is 5mg/mL, 2.5mg/mL, 1.25mg/mL, ConA skullcapflavone crude extracts pair
The conversion ratio answered is：0.038、1.507、1.317.The result shows that 1 gained skullcapflavone crude extract of embodiment is in 1.25 ~ 5mg/mL
Concentration conditions under have the effect of improve immunity of organisms.
Embodiment 3
Improve rat immunity power test experience
It tests the 0th day, 21 Laboratory Rats is divided into radix scutellariae group, cyclophosphamide-a control group, blank control group, each organize each 7
Only, every group of mouse blood is acquired respectively, carries out leucocyte（WBC）, neutrophil leucocyte（Gran）, lymphocyte（Lym）After measurement,
In addition to blank control, cyclophosphamide 50mg/kg is injected, 1 times/day, is used in conjunction 3 days.Acquisition sample measures each group respectively within 7th day
WBC, Gran, Lym index, same day radix scutellariae group gavage skullcapflavone crude extract 200mg/kg after sampling, other group of gavage equivalent life
Brine is managed, 1 times/day, is used in conjunction 28 days, as a result as shown in Figs. 1-3, the situation of change of WBC, Gran and Lym is shown respectively.
From the above results, it can be seen that becoming of rising after falling before is presented in 3 immune indexes of radix scutellariae group and cyclophosphamide-a control group
Gesture declines for 0-7 days, rises within 7-35 days.Radix scutellariae group the 7-14 days, ascendant trend is apparent, be more than blank control group, 0 day with the 7th day
Comparing difference is notable（p<0.05）, the 7th day with the 14th day poor heteropolar significantly (p<0.01).Drug cyclophosphamide control group
The trend gradually risen is presented within 7-35 days, is not above blank control group, 0 day notable with the 7th day comparing difference（p<0.05）.
Index to the 28th day each group tends to blank control group, and body has restored normal.It can be seen that gavage is yellow after rat immunity inhibition
A kind of reed mentioned in ancient books flavones coarse extraction can quickly swash intravital immune system, adjust body's immunity.
The basic principles, main features and advantages of the present invention have been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by claims and its equivalent
It defines.
Claims (2)
1. a kind of application of skullcapflavone crude extract in preparing veterinary drug, which is characterized in that skullcapflavone crude extract is for enhancing
Medicated premix in the veterinary drug of animal body immune function, the skullcapflavone crude extract is extracted from scullcap plant to be obtained,
Drug concentration of the skullcapflavone crude extract in veterinary drug is 1.25 ~ 5mg/mL.
2. application of the skullcapflavone crude extract according to claim 1 in preparing veterinary drug, which is characterized in that the Huang
A kind of reed mentioned in ancient books flavones crude extract is made by the steps：
（1）The radix scutellariae rhizome dried is smashed it through into 40 mesh sieve, obtains radix scutellariae powder；
（2）Radix scutellariae powder is taken, by feed liquid mass ratio 1:（55-65）60% ethyl alcohol of concentration is added to impregnate, stands overnight, ultrasonic power
10-20min is extracted under the conditions of 280-320W, in 65-75 DEG C of water-bath water-bath 2.5-3.5 hours；
（3）It is filtered with vacuum pump and removes the dregs of a decoction, then ethyl alcohol is flung to through depressurizing Rotary Evaporators, be put into thermostatic drying chamber control and exist
It is dry under the conditions of 65-75 DEG C, obtain the skullcapflavone crude extract that purity content is 2.5-3%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810854617.8A CN108619236A (en) | 2018-07-30 | 2018-07-30 | Application of the skullcapflavone crude extract in preparing veterinary drug |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810854617.8A CN108619236A (en) | 2018-07-30 | 2018-07-30 | Application of the skullcapflavone crude extract in preparing veterinary drug |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108619236A true CN108619236A (en) | 2018-10-09 |
Family
ID=63690095
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810854617.8A Pending CN108619236A (en) | 2018-07-30 | 2018-07-30 | Application of the skullcapflavone crude extract in preparing veterinary drug |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108619236A (en) |
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2018
- 2018-07-30 CN CN201810854617.8A patent/CN108619236A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 梁英等: "黄芩黄酮对肉仔鸡生长性能和免疫功能的影响", 《动物营养学报》 * |
| 石春红等: "超声波法提取黄芩中总黄酮的研究", 《安徽农业科学》 * |
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| PB01 | Publication | ||
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181009 |
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| RJ01 | Rejection of invention patent application after publication |