CN105941160B - A kind of breeding method of the bletilla striata - Google Patents
A kind of breeding method of the bletilla striata Download PDFInfo
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- CN105941160B CN105941160B CN201610436529.7A CN201610436529A CN105941160B CN 105941160 B CN105941160 B CN 105941160B CN 201610436529 A CN201610436529 A CN 201610436529A CN 105941160 B CN105941160 B CN 105941160B
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- bletilla striata
- bulb
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
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Abstract
The present invention provides a kind of breeding method of the bletilla striata, including：Bletilla striata bulb is inoculated in culture medium and is cultivated, the culture medium includes MS culture mediums, sucrose, agar and DA 6.The present invention adds sucrose and DA 6 for cultivating bletilla striata bulb in MS culture mediums, can significantly improve the performance parameters such as the survival rate of bletilla striata seedling, planting percent, height of seedling, stem thick, the number of blade, blade quality, radical, root long, root quality, bulb weight, bulb diameter, bulb quality, complete stool weight.The experimental results showed that, breeding method provided by the invention can improve the survival rate of bletilla striata seedling, planting percent, height of seedling, stem thick, the number of blade, blade quality, radical, root long, root quality, bulb weight, bulb diameter, bulb quality, complete stool weight etc., and with the optium concentration that the sucrose combination 8mg/L DA 6 of 60g/L are cultivation bletilla striata test tube seedling, carry out the cultivation of strong sprout in production to bletilla striata test tube seedling using the concentration.
Description
Technical field
The invention belongs to planting technical field more particularly to a kind of breeding methods of the bletilla striata.
Background technology
The bletilla striata (Bletill a striata (Thunb.) Reichb.f.] also known as sweet, it is Lian Jicao, purple blue, be that orchid family is white
Splendid achnatherum category herbaceos perennial.Yin Qigen for white, and it is multiple connect together, so the name bletilla striata.Bletilla striata flower is generally large, face
Color is bright-coloured, and white, blue, yellow, powder and the colors such as purplish red can do potting as ornamental plant, arrange flowers.Wherein pale reddish brown trident bletilla striata bulb
Forked in three, flower is purple, and it is also known as the pale reddish brown trident bletilla striatas.The bletilla striata florescence, fruit pod was in 7~August, fruit pod generally in 4~May
Ripe harvest time is generally by the end of November.The bletilla striata is all distributed in the world from the Upper Myanmar in Asia through China to Japan.The bletilla striata
The weather of warm and moist is liked, is frequently grown in the moss layer, small stream curb wall or hayashishita wetland of relatively moistening, the slightly cold-resistant happiness of the bletilla striata is cloudy
Cool, shade tolerance is strong, and cultivation soil is best with well-drained sand loam.Distribution Area annual rainfall of the bletilla striata in China exists
More than 1100mm, 18~20 DEG C of average temperature of the whole year, minimum 8~10 DEG C of daily mean temperature, the relative humidity of air for 70%~
80%.The bletilla striata also has high medical value, early in《Sheng Nong's herbal classic》In it is on the books[6].Research in recent years represents that the bletilla striata contains
Join the luxuriant and rich with fragrance class of Kaifeng class, dihydro phenanthrene class, connection and other containing chemical compositions such as phenanthrene compounds, it is mainly that bletilla striata connection is luxuriant and rich with fragrance to be currently known the luxuriant and rich with fragrance class of connection
A, B, C and luxuriant and rich with fragrance alcohol A, B, the C of bletilla striata connection[7~8].The bletilla striata has hemostasis, the protective effects on gastric mucosa；Can antibacterial, anticancer it is antitumor；And
It plays an important role for skin trauma immediate union.It can clinically treat：Inside and outside haemorrhage, burn, scald, tumour.Also
The expression that immune-regulating factor can be induced, the activity for enhancing natural killer cell, the phagocytosis work(for promoting reticuloendothelial system
Can etc..The bletilla striata can also be used for preparing soluble facial mask.Research shows that bletilla polysaccharide is a kind of stickiness polysaccharide, film forming is good, can
To form one layer of uniform film in skin surface, it is possible to reduce moisture of skin evaporates, and makes the further aquation of cuticula and then preservation
Moisture of skin.
Traditional modes of reproduction of the bletilla striata has seminal propagation and division propagation, and actual production is mainly based on division propagation.In vain
Splendid achnatherum seed is very tiny, and necessary symbiotic effects is needed to exist in its natural state could seedling.The division propagation of the bletilla striata is general
It is carried out in spring or autumn and winter, i.e., before ground young leaves is sprouted or after old leaf withers, the pseudobulb with top is usually divided into 3
~5 parts carry out a point plant.The plant strain growth period bred using old strain pseudobulb is longer, generally requires 2~4 years.Tissue cultures skill
Art can make the bletilla striata and other orchids quickly breed, shorten the plant strain growth period, be also beneficial to preserving seed and cultivate new
Kind, so as to make nursery level of effort commercialization.In recent years, due to the growth of bletilla striata economic value, wild bletilla striata is by serious
It is private dig coyoting, development bletilla striata tissue culture technology is necessary.The research to bletilla striata tissue cultures domestic at present have it is certain into
Exhibition, the explant of use is mostly seed.Such as leaf waits quietly establishing the bletilla striata rapid propagation system for carrying out aseptic seeding using seed, and
Successfully obtain aseptic seedling.But the bletilla striata seedling various performance parameters that this method is cultivated are unsatisfactory.
Invention content
In view of this, the purpose of the present invention is to provide a kind of breeding method of the bletilla striata, method provided by the invention is cultivated
It can improve that the survival rate of bletilla striata seedling, height of seedling, stem be thick, the number of blade, blade quality, radical, root long, root quality, bulb weight, squama
Stem quality, the complete stool performance parameters such as again.
The present invention provides a kind of breeding method of the bletilla striata, including：
Bletilla striata bulb is inoculated in culture medium and is cultivated, the culture medium include MS culture mediums, sucrose, agar and
DA-6。
The present invention adds sucrose in MS culture mediums and DA-6 is used for the cultivation of the bletilla striata, can improve surviving for bletilla striata seedling
Rate, height of seedling, stem are thick, the number of blade, blade quality, radical, root long, root quality, bulb weight, bulb quality, the complete stool parameters such as again.
Culture medium provided by the invention includes MS culture mediums, and the present invention is not particularly limited the MS culture mediums, ability
The MS culture mediums that field technique personnel are well known, buy in the market.
Culture medium provided by the invention includes agar, and the concentration of the agar is preferably 4g/L~5g/L, more preferably
4.8g/L。
Culture medium provided by the invention includes sucrose, and the concentration of the sucrose is preferably 30g/L~90g/L, most preferably
60g/L。
Culture medium provided by the invention includes DA-6, and the concentration of the DA-6 is preferably 4~12mg/L, more preferably 4mg/
L, 8mg/L and 12mg/L, most preferably 8mg/L.
In one embodiment, in the culture medium, a concentration of 60g/L of the sucrose, a concentration of 8mg/L of DA-6.
In the present invention, the pH value of the culture medium is 5.6~5.8.
Bletilla striata bulb is inoculated in above-mentioned culture medium and cultivates by the present invention, and the temperature of the cultivation is preferably 20 DEG C~
25 DEG C, more preferably 23 DEG C；The intensity of illumination of the cultivation is preferably 1500Lx~2500Lx, more preferably 2000Lx；It is described
The lighting delay number of cultivation is preferably 10h~20h, more preferably 16h.
In the present invention, the bletilla striata bulb is preferably the bulb of bletilla striata test tube seedling, and the bletilla striata test tube seedling is preferably purple
Flower trident bletilla striata test tube seedling.
Bletilla striata bulb is cultivated in the medium, replaces culture medium after emergence as required, then by the small of acquisition
In transplantation of seedlings to compost seed plate in greenhouse hardening, then be placed in timbered soil and grow naturally, can be received after maturation after 3 years
It obtains.
The present invention provides a kind of breeding method of the bletilla striata, including：Bletilla striata bulb is inoculated in culture medium and is cultivated,
The culture medium includes MS culture mediums, sucrose, agar and DA-6.The present invention adds sucrose and DA-6 for training in MS culture mediums
Educate bletilla striata bulb, can significantly improve the thick survival rate of bletilla striata seedling, planting percent, height of seedling, stem, the number of blade, blade quality, radical,
The performance parameters such as root long, root quality, bulb weight, bulb diameter, bulb quality, complete stool weight.The experimental results showed that the present invention
The breeding method of offer can improve that the survival rate of bletilla striata seedling, planting percent, height of seedling, stem be thick, the number of blade, blade quality, radical, root
Length, root quality, bulb weight, bulb diameter, bulb quality, complete stool weight etc., and with the sucrose concentration and 8mg/L of 60g/L
DA-6 be cultivate bletilla striata test tube seedling optium concentration, in production using the concentration to bletilla striata test tube seedling carry out strong sprout training
It educates.
Description of the drawings
Fig. 1 is the bletilla striata Bulb culture growing state of 30 days provided in an embodiment of the present invention；
Fig. 2 is the bletilla striata Bulb culture growing state of 60 days provided in an embodiment of the present invention.
Specific embodiment
The technical solution in the embodiment of the present invention will be clearly and completely described below, it is clear that described implementation
Example is only part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's all other embodiments obtained without making creative work belong to the model that the present invention protects
It encloses.
1. materials and methods
1.1 experiment material
Experiment material is pale reddish brown trident bletilla striata test tube seedling, is provided by plant physiology system of Sichuan Agricultural University tissue culture room.
1.2 experimental design
This experiment adds various concentration sucrose (30g/L, 60g/L, 90g/L) and difference is dense using MS as minimal medium
The DA-6 (4mg/L, 8mg/L, 12mg/L) of degree is matched, totally 12 processing groups, and to be not added with DA-6, as control, (matched proportion density is shown in Table
1), agar concentration 4.8g/L, pH are 5.6~5.8, are respectively connected to pale reddish brown trident bletilla striata test tube seedling.
1.3 experimental method
The consistent bletilla striata test tube seedling of seedling age is cut off into aerial part, by bulb of the same size access various concentration sucrose
Culture medium, 2 bletilla striata bulbs of every bottle of inoculation, each sucrose concentration is inoculated with 30 altogether, using 10 bulbs as a repetition, if 3 times
It repeats, is inoculated with 150 bletilla striata bulbs altogether.The culture medium being inoculated be placed on culturing room temperature be (23 ± 2) DEG C, intensity of illumination
Observation culture, record test tube seedling growing state and statistical data are carried out under the conditions of 2000Lx, lighting delay number 16h/d.Measurement index：
Be inoculated with strain number, survival rate, height of seedling, stem thick, the number of blade, blade quality, radical, root long, root quality, bulb weight, bulb quality,
Bulb quality, complete stool weight.Experimental data using average, counts after cultivating 60d.
Experimental data use carries out the average significance of difference with t inspections and compares.Wherein percentage carries out arcsine turn
It tests again after changing.
2. experimental result and analysis
Influence of the 2.1 different sucrose and DA-6 concentration to bletilla striata bulb growth
The statistical analysis of table 1 different sucrose and DA-6 concentration to bletilla striata bulb growth situation
(note：Level of signifiance a=0.05, similarly hereinafter.)
Interpretation of result is it is found that add 60g/L sucrose and 8mg/L DA-6 best results in culture medium.
As shown in Table 1, it is cultivated under 12 kinds of different sucrose and DA-6 concentration proportionings, the survival rate and planting percent of test tube seedling have
Institute is different, but higher.In sucrose concentration number is survived for the induction of bletilla striata bulb under 60g/L and DA-68g/L concentration proportionings most
It is more, inductivity 100.00%；Planting percent also highest is 90%.
2.2 sucrose and DA-6 concentration are to bletilla tissue culture seedlings overground part growth effect
The statistical analysis of 2 sucrose of table and DA-6 concentration to bletilla tissue culture seedlings overground part growing state
As can be seen from Table 2,12 kinds of sucrose and DA-6 various concentrations influence bletilla striata test tube seedling aerial growth situation
Difference, but preferably.Height of seedling highest when sucrose concentration is 60g/L and 8mg/LDA-6, is 2.45cm, the number of blade is also most, is
3.11, blade is more roomy and open and flat, and color is below this combination in each experimental group height of seedling of green.It can be it follows that with sucrose
A concentration of 60g/L and DA-6 8mg/L are the optium concentration for cultivating bletilla tissue culture seedlings.
2.3 different sucrose and DA-6 various concentrations match the influence to bletilla tissue culture seedlings complete stool weight
The statistical analysis of 3 bletilla striata test tube seedling complete stool growing state of table
From table 3 it can be seen that bletilla striata test tube seedling complete stool weight numerical value change under the influence of different sucrose and DT-6 concentration
It is larger.There is maximum value when sucrose concentration is matched for 60g/L and DT-6 concentration 8mg/L in complete stool weight, is 412.73mg, other
Processing combination is below this processing costs.
Referring to Fig. 1 and Fig. 2, Fig. 1 is the bletilla striata Bulb culture growing state of 30 days provided in an embodiment of the present invention, wherein left
It is growing state of the bletilla striata bulb in the culture medium of sucrose 30g/L in the culture bottle of side, right side culture bottle is bletilla striata bulb in sugarcane
Growing state in the culture medium of sugared 30g/L and DA-68mg/L.Fig. 2 is bletilla striata Bulb culture 60 provided in an embodiment of the present invention
It growing state, wherein be growing state of the bletilla striata bulb in the culture medium of sucrose 30g/L in the culture bottle of left side, right side training
It is growing state of the bletilla striata bulb in the culture medium of sucrose 30g/L and DA-68mg/L to support bottle.
Analysis of experimental results shows：As shown in every testing index, breeding method provided by the invention can improve the bletilla striata
The survival rate of seedling, planting percent, height of seedling, stem are thick, the number of blade, blade quality, radical, root long, root quality, bulb weight, bulb are straight
Diameter, bulb quality, complete stool weight etc., and using the sucrose concentration proportioning 8mg/L DA-6 of 60g/L as cultivation bletilla striata test tube seedling
Optium concentration combines, and carries out the cultivation of strong sprout in production to bletilla striata test tube seedling using the concentration.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (7)
1. a kind of breeding method of the bletilla striata, including：
Bletilla striata bulb is inoculated in culture medium and is cultivated, the culture medium includes MS culture mediums, sucrose, agar and DA-6；
A concentration of 4~12mg/L of a concentration of 30g/L~90g/L of the sucrose, DA-6.
2. breeding method according to claim 1, which is characterized in that a concentration of 60g/L of the sucrose, DA-6 are a concentration of
8mg/L。
3. breeding method according to claim 1, which is characterized in that a concentration of 4g/L~5g/L of the agar.
4. breeding method according to claim 1, which is characterized in that the pH value of the culture medium is 5.6~5.8.
5. breeding method according to claim 1, which is characterized in that the temperature of the cultivation is 20 DEG C~25 DEG C.
6. breeding method according to claim 1, which is characterized in that the intensity of illumination of the cultivation for 1500Lx~
2500Lx。
7. breeding method according to claim 1, which is characterized in that the lighting delay number of the cultivation is 10h~20h.
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| CN201610436529.7A CN105941160B (en) | 2016-06-17 | 2016-06-17 | A kind of breeding method of the bletilla striata |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103477984A (en) * | 2013-09-09 | 2014-01-01 | 华南农业大学 | Culture medium and culture method for promoting growth of regeneration buds of echinacea purpurea |
| CN104920208A (en) * | 2015-05-15 | 2015-09-23 | 浙江虹越花卉股份有限公司 | Culture medium for rapid propagation in tissue culture of bletilla striata |
| CN105052752A (en) * | 2015-09-17 | 2015-11-18 | 康美药业（文山）药材种植管理有限公司 | Culture medium for bletilla striata tissue culture |
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- 2016-06-17 CN CN201610436529.7A patent/CN105941160B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103477984A (en) * | 2013-09-09 | 2014-01-01 | 华南农业大学 | Culture medium and culture method for promoting growth of regeneration buds of echinacea purpurea |
| CN104920208A (en) * | 2015-05-15 | 2015-09-23 | 浙江虹越花卉股份有限公司 | Culture medium for rapid propagation in tissue culture of bletilla striata |
| CN105052752A (en) * | 2015-09-17 | 2015-11-18 | 康美药业（文山）药材种植管理有限公司 | Culture medium for bletilla striata tissue culture |
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