CN105918535A - Hibiscus-manihot healthful tea and preparing method and application thereof - Google Patents
Hibiscus-manihot healthful tea and preparing method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/14—Tea preparations, e.g. using additives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/286—Carthamus (distaff thistle)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/62—Nymphaeaceae (Water-lily family)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/82—Theaceae (Tea family), e.g. camellia
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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Abstract
The invention belongs to the field of health care products, and particularly relates to hibiscus-manihot healthful tea and a preparing method and application thereof. The hibiscus-manihot healthful tea mainly comprises hibiscus manihot, safflower, lotus seeds, gorgon fruits, angelica sinensis, sophora flowers, coreopsis lanceolata, mesona chinensis, angelica roots, green tea and rock candy. The hibiscus-manihot healthful tea is reasonable and scientific in compatibility, and as the coordination effect of all the components is carried out, the effects of relieving the liver and depression, relaxing qi, activating blood, tonifying qi, reinforcing vigor, nourishing the liver and the kidney and resisting to bacteria and viruses are achieved. An experiment shows that by means of the hibiscus-manihot healthful tea, the phagocytosis of Raw 264.7 cells can be effectively improved, the survival time of mouse load swimming is prolonged, and the immunologic function of the human body can be effectively improved.
Description
Technical field
The invention belongs to field of health care products, be specifically related to a kind of Hibiscus manihot L. health preserving tea and its production and use.
Background technology
Along with people live life raising and to healthy pay attention to day by day, get growing concern for the method for tea health preserving.
Health preserving tea not only can also have the effect for the treatment of relevant disease with health preserving, sells in the market and has various health preserving tea,
Such as: there is the Semen Cassiae haw tea of slimming function, there is the Flos Chrysanthemi haw tea of blood fat-reducing blood pressure-decreasing effect, there is heat clearing away solution
The gold silver chrysanthemum scented tea of poison effect, also has the green tea of effects of beautification and nourishing face, Radix Angelicae Dahuricae tea and rose-tee.Owing to health preserving tea has
Drink conveniently, side effect little, the obvious advantage of effect and favored by the consumers that get more and more.
Chinese patent CN103564105B discloses a kind of promoting digestion and invigorating the stomach health protecting tea, described promoting digestion and invigorating the stomach health protecting tea
It is made up of raw materials such as Fructus Phyllanthi, Mel, Fructus Crataegi, Pericarpium Citri Reticulatae, CitrusaurantiumL.Var.amara Engl., Fructus Hippophae, Flos Lonicerae and Semen Raphanis, prepares
Promoting digestion and invigorating the stomach health protecting tea has promoting digestion and invigorating the stomach, promoting the production of body fluid for nourishing the lung, clearing internal heat and removing dryness, intestine moistening diuresis, heat-clearing and toxic substances removing and enhancing and exempts from
The effects such as epidemic disease power, are health care and the sports drink of a kind of multi-energy type.The most also have in good taste, the advantage that toxic and side effects is little,
Be suitable to the drunk by people of each age group.
Chinese patent application 201410502129.2 discloses the health preserving tea of a kind of blood pressure lowering, and described health preserving tea is by Cortex Lycii, winter
The raw material compositions such as worm summer grass, red lantern, Ramulus et Folium Mussaendae Pubescentis, Folium Nelumbinis and Folium Ginkgo, the blood pressure lowering health preserving tea prepared has in multi-flavor
Herb ingredients, nutritious, advantage that antihypertensive effect is good, can effectively slow down the misery of hyperpietic.
Hibiscus manihot L. (Hibiseu Smanihot L.) is Malvaceae Abelmoschus plant, and it contains abundant active substance, main component
For natural flavone compounds, unsaturated fatty acid, vitamin E, hold carbohydrate gum etc. high.Finding through modern study, Hibiscus manihot L. has
There is multiple efficacies, such as: there is diuresis and glomerule protective effect;Regulation human endocrine, improve cardiovascular and cerebrovascular vessel and
Microcirculation function, the effect such as enhancing body oxidation resistance and blood fat reducing, there is higher medical value.
At present, along with to the research of Hibiscus manihot L. deeply, people start to be widely applied in health preserving health-care food field Hibiscus manihot L..
Chinese patent application 201410807173.4 discloses a kind of Hibiscus manihot L. health preserving tea and preparation method, and described Hibiscus manihot L. health preserving tea is
Hibiscus manihot L. is soaked in green tea extractive liquor, then carries out drying, complete, knead, be dried, to obtain final product, the gold prepared
Tree mallow health preserving tea has increase resistance of human body, improves cardiovascular and cerebrovascular vessel and microcirculation function, enhancing body oxidation resistance and fall
Effect of blood fat.But this Hibiscus manihot L. health preserving tea composition is more single, causes effect poor, and whole structure is the best, do not meet existing
For Man's Demands.
Summary of the invention
In order to solve the deficiency that in prior art, Hibiscus manihot L. health preserving tea exists, it is an object of the invention to provide a kind of Hibiscus manihot L. health preserving
Tea and preparation method thereof, the present invention provide Hibiscus manihot L. health preserving tea have dispersing the stagnated live-QI to relieve the stagnation of QI, have a respite invigorate blood circulation, QI invigorating by force essence, nourishing
Liver and kidney and effect of anti-bacteria and anti-virus, can significantly improve body immunity.
The invention provides a kind of Hibiscus manihot L. health preserving tea, including following raw material and the parts by weight thereof prepared:
Hibiscus manihot L. 15-30 part, Flos Carthami 12-22 part, Semen Nelumbinis 6-12 part, Semen Euryales 6-10 part, Radix Angelicae Sinensis 4-10 part, Flos Sophorae 4-10
Part, sword-like leave Coreopsis basalis 6-12 part, Mesona chinensis Benth's 10-18 part, Radix Angelicae Dahuricae 12-24 part, green tea 4-8 part and crystal sugar 4-8 part.
Further, described Hibiscus manihot L. health preserving tea includes following raw material and the parts by weight thereof prepared:
Hibiscus manihot L. 24 parts, 18 parts of Flos Carthami, 10 parts of Semen Nelumbinis, Semen Euryales 8 parts, Radix Angelicae Sinensis 6 parts, 6 parts of Flos Sophorae, sword-like leave Coreopsis basalis
8 parts, Mesona chinensis Benth 14 parts, the Radix Angelicae Dahuricae 20 parts, green tea 6 parts and 5 parts of crystal sugar.
It addition, present invention also offers the preparation method of a kind of Hibiscus manihot L. health preserving tea, comprise the following steps:
Hibiscus manihot L., Semen Nelumbinis, Semen Euryales, Flos Sophorae and Mesona chinensis Benth are cleaned by S1, add above-mentioned medical material gross weight 4-6 times water, add
Heat is extracted 2-3 time, each 1-2h, filters, merging filtrate, concentrates filtrate to relative density under the conditions of 50-54 DEG C
Thick paste for 1.20-1.25;
Flos Carthami, sword-like leave Coreopsis basalis, Radix Angelicae Sinensis and the Radix Angelicae Dahuricae are pulverized and mixed by S2, add above-mentioned coarse powder gross weight 3-5 times concentration and are
The concentration of alcohol of 65-75%, extracts 2-3 time, each 2-3h, filters, merging filtrate, filtrate is subtracted under the conditions of 50-54 DEG C
Pressure is concentrated into the thick paste that relative density is 1.20-1.25;
Green tea and crystal sugar are pulverized by S3, cross 100-200 mesh, obtain mixture;
The mixture that the thick paste that thick paste that step S1 is obtained by S4, step S2 obtain obtains with step S3 mixes, and microwave is true
Empty dry, to obtain final product.
Further, the Extracting temperature in described step S1 is 65-75 DEG C.
Further, the concentration of alcohol in described step S2 is 70%.
Further, in described step S4, the power of microwave vacuum drying is 380-420W, and vacuum is 32-40KPa, dry
The dry time is 18-25min.
Further, in described step S4, the power of microwave vacuum drying is 400W, and vacuum is 35KPa, drying time
For 20min.
In addition, present invention also offers described Hibiscus manihot L. health preserving tea purposes in the health product preparing enhancing immunity.
The present invention provide Hibiscus manihot L. health preserving tea in Hibiscus manihot L. (Hibiseu Smanihot L.) have defying age, resisting fatigue,
Effect of blood circulation promoting;Flos Carthami (Carthamus tinctorius L.) has promoting blood circulation to restore menstrual flow, the function of eliminating stasis to stop pain;Lotus
Son is the dry mature seed of nymphaeaceae plant lotus (Nelumbo nucifera Gaertn.), and Semen Euryales is nymphaeaceae plant Gorgon euryale
The dry mature kernal of (Euryale ferox Salisb.), Semen Nelumbinis and Semen Euryales have calming heart and tranquilizing mind, effect of controlling nocturnal emission with astringent drugs kidney tonifying;When
Return (Angelica sinensis) to have benefiting blood and regulating blood circulation, menstruction regulating and pain relieving, moisturize effect of laxation;Flos Sophorae (Sophora japonica
Linn) there is effect of heat clearing away, removing heat from blood, hemostasis, blood pressure lowering;Sword-like leave Coreopsis basalis (Coreopsis lanceolata L.) has clearly
The effect such as thermal detoxification and blood pressure lowering;The Radix Angelicae Dahuricae (Angelica dahurica (Fisch.ex Hoffm.) Benth.et Hook.f.ex Franch.
Et Sav) have dispel the wind, dampness and the effect of pain relieving;Mesona chinensis Benth (Mesona chinensis Benth.) has clearing away heat-damp and promoting diuresis,
Removing heat from blood expelling summer-heat, effect of removing toxic substances.Green tea is the most fermented tea made, and remains the natural materials of fresh leaf, containing enrich
Tea polyphenols, catechin, chlorophyll, caffeine, aminoacid, vitamins and other nutritious components.
Each component of the Hibiscus manihot L. health preserving tea that the present invention provides interact work in coordination with dispersing the stagnated live-QI to relieve the stagnation of QI, have a respite invigorate blood circulation, QI invigorating by force smart,
Nourishing the liver and kidney and effect of anti-bacteria and anti-virus.Finding through test, the Hibiscus manihot L. health preserving tea that the present invention provides can be effectively improved Raw
The phagocytosis of 264.7 cells, the Raw 264.7 cultivated in the culture medium being added with the Hibiscus manihot L. health preserving tea that the present invention provides
The phagocytic index of cell is more than 1.426, illustrates that the Hibiscus manihot L. health preserving tea that the present invention provides can effectively strengthen body immunity.
Further, the Hibiscus manihot L. health preserving tea that the present invention provides can effectively extend the time-to-live of mice burden swimming time,
Improve physical ability and the immunity of mice.Finding through test, the time-to-live of the walking weight load of control group mice is 5.8min,
And the mean survival time of the mice burden swimming of embodiment of the present invention 1-3 group is more than 14.1min, greatly extends mice and bear
The time-to-live of weight swimming time.Meanwhile, the Hibiscus manihot L. health preserving tea that the present invention provides also has and significantly improves body immunity
Function, its total improvement rate reaches 96.67%, is a kind of ideal health preserving tea strengthening body immunity.
Compared with prior art, the Hibiscus manihot L. health preserving tea that the present invention provides has the advantage that
(1) the Hibiscus manihot L. health preserving tea steady quality that the present invention provides, clear efficacy, the suitability is wide, is suitable for different local and different
Drunk by people, break through season and the restriction of region;
(2) the Hibiscus manihot L. health preserving tea that the present invention provides have dispersing the stagnated live-QI to relieve the stagnation of QI, have a respite invigorate blood circulation, QI invigorating essence by force, nourishing the liver and kidney and antibacterial anti-
Effect of virus, can significantly improve body immunity.
Detailed description of the invention:
Below by way of the description of detailed description of the invention, the invention will be further described, but this is not limitation of the present invention,
Those skilled in the art are according to the basic thought of the present invention, and various modifications may be made or improves, but without departing from this
Bright basic thought, the most within the scope of the present invention.
Embodiment 1, a kind of Hibiscus manihot L. health preserving tea
Described Hibiscus manihot L. health preserving tea is by following raw material and the parts by weight thereof prepared:
Hibiscus manihot L. 15 parts, 12 parts of Flos Carthami, 6 parts of Semen Nelumbinis, Semen Euryales 6 parts, Radix Angelicae Sinensis 4 parts, 4 parts of Flos Sophorae, sword-like leave Coreopsis basalis
6 parts, Mesona chinensis Benth 10 parts, the Radix Angelicae Dahuricae 12 parts, green tea 4 parts and 4 parts of crystal sugar.
Preparation method:
Hibiscus manihot L., Semen Nelumbinis, Semen Euryales, Flos Sophorae and Mesona chinensis Benth are cleaned by S1, add 4 times of water of above-mentioned medical material gross weight, are heated to
65 DEG C are extracted 2 times, and each 1h filters, and merging filtrate, concentrating filtrate to relative density under the conditions of 50 DEG C is 1.20
Thick paste;
Flos Carthami, sword-like leave Coreopsis basalis, Radix Angelicae Sinensis and the Radix Angelicae Dahuricae are pulverized and mixed by S2, and adding 3 times of concentration of above-mentioned coarse powder gross weight is 65%
Ethanol solution, extract 2 times, each 2h, filter, merging filtrate, concentrate filtrate to relatively under the conditions of 50 DEG C
Density is the thick paste of 1.20;
Green tea and crystal sugar are pulverized by S3, cross 100 mesh, obtain mixture;
The mixture that the thick paste that thick paste that step S1 is obtained by S4, step S2 obtain obtains with step S3 mixes, and microwave is true
Empty dry, described dry power is 380W, and vacuum is 32KPa, and drying time is 18min, to obtain final product.
Embodiment 2, a kind of Hibiscus manihot L. health preserving tea
Described Hibiscus manihot L. health preserving tea is by following raw material and the parts by weight thereof prepared:
Hibiscus manihot L. 24 parts, 18 parts of Flos Carthami, 10 parts of Semen Nelumbinis, Semen Euryales 8 parts, Radix Angelicae Sinensis 6 parts, 6 parts of Flos Sophorae, sword-like leave Coreopsis basalis
8 parts, Mesona chinensis Benth 14 parts, the Radix Angelicae Dahuricae 20 parts, green tea 6 parts and 5 parts of crystal sugar.
Preparation method:
Hibiscus manihot L., Semen Nelumbinis, Semen Euryales, Flos Sophorae and Mesona chinensis Benth are cleaned by S1, add 5 times of water of above-mentioned medical material gross weight, are heated to
70 DEG C are extracted 2 times, and each 1h filters, and merging filtrate, concentrating filtrate to relative density under the conditions of 52 DEG C is 1.23
Thick paste;
Flos Carthami, sword-like leave Coreopsis basalis, Radix Angelicae Sinensis and the Radix Angelicae Dahuricae are pulverized and mixed by S2, and adding 4 times of concentration of above-mentioned coarse powder gross weight is 70%
Ethanol solution, extract 2 times, each 2h, filter, merging filtrate, concentrate filtrate to relatively under the conditions of 52 DEG C
Density is the thick paste of 1.23;
Green tea and crystal sugar are pulverized by S3, cross 140 mesh, obtain mixture;
The mixture that the thick paste that thick paste that step S1 is obtained by S4, step S2 obtain obtains with step S3 mixes, and microwave is true
Empty dry, described dry power is 400W, and vacuum is 35KPa, and drying time is 20min, to obtain final product.
Embodiment 3, a kind of Hibiscus manihot L. health preserving tea
Described Hibiscus manihot L. health preserving tea is by following raw material and the parts by weight thereof prepared:
Hibiscus manihot L. 30 parts, 22 parts of Flos Carthami, 12 parts of Semen Nelumbinis, Semen Euryales 10 parts, Radix Angelicae Sinensis 10 parts, 10 parts of Flos Sophorae, sword-like leave gold
Chicken chrysanthemum 12 parts, Mesona chinensis Benth 18 parts, the Radix Angelicae Dahuricae 24 parts, green tea 8 parts and 8 parts of crystal sugar.
Preparation method:
Hibiscus manihot L., Semen Nelumbinis, Semen Euryales, Flos Sophorae and Mesona chinensis Benth are cleaned by S1, add 6 times of water of above-mentioned medical material gross weight, are heated to
75 DEG C are extracted 3 times, and each 2h filters, and merging filtrate, concentrating filtrate to relative density under the conditions of 54 DEG C is 1.25
Thick paste;
Flos Carthami, sword-like leave Coreopsis basalis, Radix Angelicae Sinensis and the Radix Angelicae Dahuricae are pulverized and mixed by S2, and adding 5 times of concentration of above-mentioned coarse powder gross weight is 75%
Ethanol solution, extract 3 times, each 3h, filter, merging filtrate, concentrate filtrate to relatively under the conditions of 54 DEG C
Density is the thick paste of 1.25;
Green tea and crystal sugar are pulverized by S3, cross 200 mesh, obtain mixture;
The mixture that the thick paste that thick paste that step S1 is obtained by S4, step S2 obtain obtains with step S3 mixes, and microwave is true
Empty dry, described dry power is 420W, and vacuum is 40KPa, and drying time is 25min, to obtain final product.
Comparative example 1, a kind of Hibiscus manihot L. health preserving tea
Described Hibiscus manihot L. health preserving tea is by following raw material and the parts by weight thereof prepared:
Hibiscus manihot L. 24 parts, 18 parts of Flos Carthami, 10 parts of Semen Nelumbinis, Semen Euryales 8 parts, Radix Angelicae Sinensis 6 parts, 6 parts of Flos Sophorae, Flos Chrysanthemi 8 parts,
Mesona chinensis Benth 14 parts, the Radix Angelicae Dahuricae 20 parts, green tea 6 parts and 5 parts of crystal sugar.
Preparation method is similar to Example 2.
Difference with embodiment 2 is, sword-like leave Coreopsis basalis is replaced with Flos Chrysanthemi.
Comparative example 2, a kind of Hibiscus manihot L. health preserving tea
Described Hibiscus manihot L. health preserving tea is by following raw material and the parts by weight thereof prepared:
Hibiscus manihot L. 27 parts, 21 parts of Flos Carthami, 10 parts of Semen Nelumbinis, Semen Euryales 8 parts, Radix Angelicae Sinensis 6 parts, 6 parts of Flos Sophorae, sword-like leave Coreopsis basalis 8
Part, Mesona chinensis Benth 14 parts, the Radix Angelicae Dahuricae 20 parts, green tea 6 parts and 5 parts of crystal sugar.
Preparation method is similar to Example 2.
Difference with embodiment 2 is, is not added with Flos Sophorae, increases the parts by weight of Hibiscus manihot L. Flos Carthami.
The impact of phagocytes in vitro is tested by test example one, Hibiscus manihot L. health preserving tea
1, subjects: Raw 264.7 cell line, is provided by Chinese Academy of Sciences's Shanghai cell bank.
2, test material: embodiment 1, embodiment 2, embodiment 3, comparative example 1 and the Hibiscus manihot L. health preserving tea of comparative example 2 preparation;
Phorbol 12-myristinate 13-acetas (PMA), No. CAS is 16561-29-8;Latex bead (3 μm), by Germany
Polysciences company provides;Geimsa stain, is provided by Beijing Hua Maike biotechnology Co., Ltd.
3, test method:
Raw 264.7 cell line, they every 2-3 are cultivated continuously in the DMEM culture medium containing 10%FCS (hyclone)
It passes on once, at 5%CO2, cultivate at 37 DEG C.Then with 105The density of cell/cover glass coats on the cover slip
Raw 264.7 cell, and make it adhere to, will be added with 100ng/ml PMA, embodiment 1, embodiment 2, embodiment 3,
The culture medium of the Hibiscus manihot L. health preserving tea combination of comparative example 1 and comparative example 2 preparation cultivates cell 2h, described Hibiscus manihot L. health preserving tea
Addition is the 0.05% of culture medium cumulative volume.After cultivation, with 106Latex bead/105Cell (cell: pearl=1: 10) is cultivated thin
Born of the same parents 2h, air-dries the cell on coverslip, and fixes cell with methanol.Cell, after Giemsa dyeing 10min, uses microscope
Observing, calculate the phagocytosis index of macrophage, wherein phagocytosis index refers to the intracellular pearl of every 100 cells
Sum.
4, result of the test:
Result of the test is as shown in table 1.
The impact of phagocytes in vitro is tested by table 1 Hibiscus manihot L. health preserving tea
Group | Phagocytosis index |
Embodiment 1 | 1.40±0.46 |
Embodiment 2 | 1.46±0.50 |
Embodiment 3 | 1.42±0.48 |
Comparative example 1 | 0.85±0.35 |
Comparative example 2 | 0.78±0.34 |
As shown in Table 1, the Raw 264.7 of the culture medium culturing of Hibiscus manihot L. health preserving tea prepared by embodiment of the present invention 1-3 it is added with
The average phagocytic index of cell is more than 1.426, and wherein the average phagocytic index of Raw 264.7 cell of embodiment 2 is 1.46,
For most preferred embodiment.And be added with Raw 264.7 cell of the culture medium culturing of Hibiscus manihot L. health preserving tea prepared by comparative example 1-2
Average phagocytic index, less than 0.85, illustrates that the Hibiscus manihot L. health tea formula that the present invention provides is reasonable, and science, each component is worked in coordination with
The effect of enhancing immunity.
The impact of mice burden swimming time is tested by test example two, Hibiscus manihot L. health preserving tea
1, subjects: choose 60 mices, body weight is 18-22g, Zhongshan University's Experimental Animal Center provide.
2, test material: embodiment 1, embodiment 2, embodiment 3, comparative example 1 and the Hibiscus manihot L. health preserving tea of comparative example 2 preparation.
3, test method:
60 mices are randomly divided into 6 groups, often group 10, respectively matched group, embodiment 1 group, embodiment 2 groups, enforcement
Example 3 groups, comparative example 1 group and comparative example 2 groups, each group dosage is as follows:
Matched group: the isopyknic normal saline of gavage;
Embodiment 1 group: the Hibiscus manihot L. health preserving tea of embodiment 1 preparation of gavage 0.75g/kg;
Embodiment 2 groups: the Hibiscus manihot L. health preserving tea of embodiment 2 preparation of gavage 0.75g/kg;
Embodiment 3 groups: the Hibiscus manihot L. health preserving tea of embodiment 3 preparation of gavage 0.75g/kg;
Comparative example 1 group: the Hibiscus manihot L. health preserving tea of comparative example 1 preparation of gavage 0.75g/kg;
Comparative example 2 groups: the Hibiscus manihot L. health preserving tea of comparative example 2 preparation of gavage 0.75g/kg;
Every day 1 time, successive administration 40 days, after last is administered 30min, the mice of root of the tail portion load 5% body weight sheet lead is placed in
Depth of water 30cm, the swimming trunk went swimming of water temperature 25 ± 0.5 DEG C, record mice starts to dead time, i.e. mice to bear from swimming
Weight swimming time.
4, result of the test;
Result of the test is as shown in table 2.
The impact of mice burden swimming time is tested by table 2 Hibiscus manihot L. health preserving tea
Group | Swimming with a load attached to the body mean survival time (min) |
Matched group | 5.8 |
Embodiment 1 group | 13.6 |
Embodiment 2 groups | 14.8 |
Embodiment 3 groups | 13.9 |
Comparative example 1 group | 8.2 |
Comparative example 2 groups | 7.4 |
As shown in Table 2, the mice burden swimming mean survival time of embodiment of the present invention 1-3 group is 14.1min, wherein implements
The swimming with a load attached to the body mean survival time of 2 groups of mices of example is 14.8min, for most preferred embodiment.And the mice of comparative example 1-2 group is born
Revisit swimming mean survival time and be less than 8.2min, illustrate that each component of Hibiscus manihot L. health preserving tea that the present invention provides has worked in coordination with prolongation mice
The time-to-live of swimming with a load attached to the body, improve physical ability and the immunity of mice.
Test example three, the Hibiscus manihot L. health preserving tea clinical efficacy to improving immune function of human body
1, test material: the Hibiscus manihot L. health preserving tea of embodiment 2 preparation.
2, tested crowd: choose 30 examples and suffer from multiple chronic disease, be diagnosed as the volunteer of syndrome of deficiency of lung qi or hypoimmunity.
3, test method:
Taking 5g Hibiscus manihot L. health preserving tea boiling water every morning to brew, drink, one day 1 time, 1 month was 1 course for the treatment of, treated continuously
3 courses for the treatment of, during treatment, volunteer is carried out blood, urine, feces routine test and the heart, pulmonary function test, and observes: heating,
Aversion to cold, sniffle, pharyngeal symptom and systemic conditions and temperature of having a medical check-up, pharyngeal tonsil, picture of the tongue pulse condition, fingerprint etc. changes,
Carry out therapeutic evaluation respectively.
4, result of the test:
Result of the test is as shown in table 3.
The table 3 Hibiscus manihot L. health preserving tea clinical efficacy to improving immune function of human body
Group | Number of cases | It is obviously improved (%) | Improve (%) | Invalid (%) | Total improvement rate (%) |
Embodiment 2 | 30 | 14 (46.67%) | 15 (50%) | 1 (3.33%) | 96.67% |
As shown in Table 3, the Hibiscus manihot L. health preserving tea that the present invention provides can be obviously improved the immunologic function of human body, its total improvement rate
Reach 96.67%, be a kind of ideal health preserving tea strengthening body immunity.
Claims (8)
1. a Hibiscus manihot L. health preserving tea, it is characterised in that include following raw material and the parts by weight thereof prepared:
Hibiscus manihot L. 15-30 part, Flos Carthami 12-22 part, Semen Nelumbinis 6-12 part, Semen Euryales 6-10 part, Radix Angelicae Sinensis 4-10 part, Flos Sophorae 4-10 part,
Sword-like leave Coreopsis basalis 6-12 part, Mesona chinensis Benth's 10-18 part, Radix Angelicae Dahuricae 12-24 part, green tea 4-8 part and crystal sugar 4-8 part.
2. Hibiscus manihot L. health preserving tea as claimed in claim 1, it is characterised in that include following raw material and the parts by weight thereof prepared:
Hibiscus manihot L. 24 parts, 18 parts of Flos Carthami, 10 parts of Semen Nelumbinis, Semen Euryales 8 parts, Radix Angelicae Sinensis 6 parts, 6 parts of Flos Sophorae, sword-like leave Coreopsis basalis 8 parts,
Mesona chinensis Benth 14 parts, the Radix Angelicae Dahuricae 20 parts, green tea 6 parts and 5 parts of crystal sugar.
3. the preparation method of a Hibiscus manihot L. health preserving tea as claimed in claim 1 or 2, it is characterised in that comprise the following steps:
Hibiscus manihot L., Semen Nelumbinis, Semen Euryales, Flos Sophorae and Mesona chinensis Benth are cleaned by S1, add above-mentioned medical material gross weight 4-6 times water, heating extraction
2-3 time, each 1-2h, filter, merging filtrate, concentrating filtrate to relative density under the conditions of 50-54 DEG C is 1.20-1.25
Thick paste;
Flos Carthami, sword-like leave Coreopsis basalis, Radix Angelicae Sinensis and the Radix Angelicae Dahuricae are pulverized and mixed by S2, and adding above-mentioned coarse powder gross weight 3-5 times concentration is 65-75%
Concentration of alcohol, extract 2-3 time, each 2-3h, filter, merging filtrate, by filtrate reduced in volume under the conditions of 50-54 DEG C
It is the thick paste of 1.20-1.25 to relative density;
Green tea and crystal sugar are pulverized by S3, cross 100-200 mesh, obtain mixture;
The mixture that the thick paste that thick paste that step S1 is obtained by S4, step S2 obtain obtains with step S3 mixes, and microwave vacuum is done
Dry, to obtain final product.
4. the preparation method of Hibiscus manihot L. health preserving tea as claimed in claim 3, it is characterised in that the Extracting temperature in described step S1
For 65-75 DEG C.
5. the preparation method of Hibiscus manihot L. health preserving tea as claimed in claim 3, it is characterised in that the concentration of alcohol in described step S2
It is 70%.
6. the preparation method of Hibiscus manihot L. health preserving tea as claimed in claim 3, it is characterised in that in described step S4, microwave vacuum is done
Dry power is 380-420W, and vacuum is 32-40KPa, and drying time is 18-25min.
7. the preparation method of Hibiscus manihot L. health preserving tea as claimed in claim 6, it is characterised in that in described step S4, microwave vacuum is done
Dry power is 400W, and vacuum is 35KPa, and drying time is 20min.
8. Hibiscus manihot L. health preserving tea purposes in the health product preparing enhancing immunity as claimed in claim 1 or 2.
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CN101253900A (en) * | 2008-03-23 | 2008-09-03 | 吴煌发 | Antiradiation Xian tea |
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CN104489159A (en) * | 2014-12-23 | 2015-04-08 | 青岛天惠御生物科技有限公司 | Abelmoschus manihot health tea and preparation method thereof |
CN105192167A (en) * | 2014-06-30 | 2015-12-30 | 吴同忠 | Chrysanthemum and flos sophorae tea for treating hypertension |
CN105558126A (en) * | 2014-11-10 | 2016-05-11 | 陶明亮 | Herbal tea |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN101253900A (en) * | 2008-03-23 | 2008-09-03 | 吴煌发 | Antiradiation Xian tea |
CN102396616A (en) * | 2011-11-05 | 2012-04-04 | 安徽省天旭茶业有限公司 | Beautifying lily beverage and preparation method thereof |
CN105192167A (en) * | 2014-06-30 | 2015-12-30 | 吴同忠 | Chrysanthemum and flos sophorae tea for treating hypertension |
CN105558126A (en) * | 2014-11-10 | 2016-05-11 | 陶明亮 | Herbal tea |
CN104489159A (en) * | 2014-12-23 | 2015-04-08 | 青岛天惠御生物科技有限公司 | Abelmoschus manihot health tea and preparation method thereof |
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