CN105911282A - 一种口腔癌特异性检测的试剂盒 - Google Patents
一种口腔癌特异性检测的试剂盒 Download PDFInfo
- Publication number
- CN105911282A CN105911282A CN201610503507.8A CN201610503507A CN105911282A CN 105911282 A CN105911282 A CN 105911282A CN 201610503507 A CN201610503507 A CN 201610503507A CN 105911282 A CN105911282 A CN 105911282A
- Authority
- CN
- China
- Prior art keywords
- bpifb2
- aptamer
- oral cancer
- dna
- kit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
- G01N2333/4701—Details
- G01N2333/4743—Bactericidal/Permeability-increasing protein BPI
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
本发明公开了一种用于口腔癌检测的试剂盒。本发明提供的核酸适体,与人BPIFB2蛋白具有较好的亲和能力。利用本发明的核酸适体,可以捕获唾液中的BPIFB2蛋白,通过其含量的变化来检测口腔癌,将其制备成为相应的试剂盒,将用于口腔癌的筛查。利用本发明的试剂盒,具有高灵敏、成本低的好处。
Description
本申请是申请日为2015年11月15日、申请号为201510775735.6、发明名称为“一种口腔癌特异性检测的试剂盒”的发明专利申请的分案申请。
技术领域
本发明涉及一种用于检测口腔癌的试剂盒及其检测方法。
背景技术
口腔癌(Oral Squarmous Cell Carcinomas,0SCC)是人体常见的肿瘤之一,尤其是在发展中国家印度、斯里兰卡、巴西等其发病率可以达到全部肿瘤的25%。近年来口腔癌的发病率在欧美等发达国家有明显的上升趋势,特别是发病年龄趋向年轻化。口腔鳞癌不仅发病率高,且恶性程度较高;往往造成语音、咀嚼、吞咽、面容等功能障碍和严重的面容毁损,并威胁到患者的生命;五年生存率只有60%左右。尽管近来口腔癌手术方法的不断改进和放疗、化疗的广泛应用,但口腔癌的五年生存率并没有明显的提高。导致这一状况的主要原因在于:口腔癌的发病机制不清,侵袭转移机制不明,缺乏有效的早期预防和预后判断手段。因此研究口腔癌发生和发展的分子机制,从中找到口腔癌早期预防、预后判断和治疗方法是口腔癌研究中急待解决的重大问题。
现有技术已知BPIFB2基因与口腔癌的关系:BPIFB2与口腔癌具有很好的相关性,可用于制备口腔癌辅助诊断或者预后制剂,具有重要的临床应用价值。其中BPIFB2在癌旁组织中的表达水平明显低于口腔癌瘤组织和对照质粒OOcopies,而在口腔癌组织中的BPIFB2高表达,即生物信息学筛选得到的BPIFB2基因与口腔癌具有很好的相关性,可用于制备口腔癌辅助诊断或者预后制剂。因此检测BPIFB2的表达变得尤为重要。
核酸适体(Aptamer,又称适配体,适配子)是能高亲和性、高特异性的结合某种生物革El标的单链寡核酸分子(ssDNA或ssRNA)。核酸适体是通过指数富集配体系统进化技术(Systemat1c Evolut1on of L1gands by Exponent1alenr1chment,SELEX)从人工合成的DNA/RNA文库中筛选得到的能够高度特异性结合靶标分子的单链DNA/RNA。已报道核酸适体的靶标包括金属离子、有机小分子、多肽、蛋白质、细胞甚至组织等。核酸适体的分子识别功能与抗体类似,具有与抗体分子相当甚至更强的靶标识别能力,但与抗体相比具有很多优良的特性,如分子量小,能批量生产,不易失活,无免疫原性、容易合成与标记、快速的穿透组织、良好的代谢动力学、不同批次之间产品不会存在差异和具有很好化学稳定性,在生物检测、疾病诊断治疗等领域具有重要的应用前景。
发明内容
本发明的目的是提供一种特异结合BPIFB2的核酸适体及其试剂盒。
本发明提供的核酸适体,是序列表的序列1-15所示的单链DNA。
所述核酸适体与BPIFB2蛋白具有较好的亲和能力。
还可将所述核酸适体进行修饰或改造,得到所述核酸适体的衍生物。
所述核酸适体的衍生物可为如下任意一种:
a)将所述核酸适体删除部分或增加部分互补的核苷酸,得到的与所述核酸适体具有相同功能的核酸适体的衍生物;
b)将所述核酸适体进行核苷酸取代或部分修饰,得到的与所述核酸适体具有相同功能的核酸适体的衍生物;
c)将所述核酸适体的骨架改造为硫代磷酸酯骨架,得到的与所述核酸适体具有相同功能的核酸适体的衍生物;
d)将核酸适体改造为肽核酸,得到的与所述核酸适体具有相同功能的核酸适体的衍生物;
e)将所述核酸适体连接上荧光、放射性和治疗性物质后,得到的与所述核酸适体具有相同功能的核酸适体的衍生物。
所述核酸适体可用于制备检测BPIFB2的试剂盒。
利用本发明的核酸适体,可以捕获中唾液中的中的BPIFB2,从而用于相关口腔癌筛查。利用本发明的核酸适体,具有高灵敏、成本低、易制备、易保存的优点。本发明具有很高的应用价值。
具体实施方式
以下的实施例便于更好地理解本发明,但并不限定本发明。下述实施例中的实验方法,如无特殊说明,均为常规方法。
实施例1BPIFB2蛋白的获得
将NP_079503所示的BPIFB2基因通过本领域常规的真核表达方式进行表达,获得了相应的目的多肽蛋白。
实施例2核酸适体的筛选和制备
设计两端包含大约20个核苷酸、中间包括41个核苷酸的随机核酸文库如下:
5‘-ACCTGATGCCATGCATCGCA(N41)CTAGCCAGCCTTTGACATCA-3’;N41代表41个随机核苷酸。
将单链DNA文库扩增为双链DNA,产物经2%琼脂糖凝胶电泳并切胶回收纯化;以回收的双链DNA为模板,体外转录出单链RNA随机文库,转录产物经PAGE纯化。75μg RNA文库经硝酸纤维素膜反筛去除与膜结合的RNA分子,然后与2ugBPIFB2蛋白,37℃孵育30min,反应液经硝酸纤维素膜滤过,洗涤滤膜;然后将滤膜剪碎,置于洗脱缓冲液(6mol/L尿素,0.55mol/L醋酸铵,l.5mmol/LEDTA,0.15%SDS)中煮沸5min,离心,取上清,无水乙醇沉淀RNA,并重新溶解于20μ1DEPC水中;以RNA为模板RT-PCR扩增双链DNA,体外转录出RNA文库用于下一轮筛选;每轮筛选过程中RT-PCR得到双链DNA文库,以该双链DNA为模板体外转录出RNA适配子库,筛选共进行11轮。得到了16个适配子,其序列分别为SEQ ID NO:1-16所示。具体序列如下所示:
BPIFB2-1:
ACCTGATGCCATGCATCGCATCACACGATCATCACACCGCTCCGCCTTCCAACTACCGCTCCTAGCCAGCCTTTGACATCA
BPIFB2-2:
ACCTGATGCCATGCATCGCACACCTCCACCCTTCGCAACTACCTCTCTTCCTGTAACTATTCTAGCCAGCCTTTGACATCA
BPIFB2-3:
ACCTGATGCCATGCATCGCATCTCATAACAAATCTTCATAATCTTCTATAACACTGTCAATCTAGCCAGCCTTTGACATCA
BPIFB2-4:
ACCTGATGCCATGCATCGCACATCTATATAGAATATATTCAATCCTCTCTCTTATATGCATCTAGCCAGCCTTTGACATCA
BPIFB2-5:
ACCTGATGCCATGCATCGCACTATCAACGACTCTCACGCAACGCTCTTCTTACTACATATACTAGCCAGCCTTTGACATCA
BPIFB2-6:
ACCTGATGCCATGCATCGCACCATTCATATCACGAACACCCATTTATACTATATACTACCACTAGCCAGCCTTTGACATCA
BPIFB2-7:
ACCTGATGCCATGCATCGCACGCACTAATATCCTTCATCCCTACTAATAACTCTTTTAACCCTAGCCAGCCTTTGACATCA
BPIFB2-8:
ACCTGATGCCATGCATCGCACACACTCATATTTATACACCAATATCATCTCACTCTATCTCCTAGCCAGCCTTTGACATCA
BPIFB2-9:
ACCTGATGCCATGCATCGCAAACCAAGAATGTCTTACGACATCTCGCCTACTATCCTATATCTAGCCAGCCTTTGACATCA
BPIFB2-10:
ACCTGATGCCATGCATCGCACACATAACAATCTATTATTCCTCTATCCGCTCTTATATTCCCTAGCCAGCCTTTGACATCA
BPIFB2-11:
ACCTGATGCCATGCATCGCAAACCAGTCTACTCAACCGACCACCTCAATATCATTCTCATCCTAGCCAGCCTTTGACATCA
BPIFB2-12:
ACCTGATGCCATGCATCGCACTACCTATACAATACTTACTGTCTGCTTCTACTTCTATTATCTAGCCAGCCTTTGACATCA
BPIFB2-13:
ACCTGATGCCATGCATCGCATATACTCGCAATCACTATATAAGACCACATAAATCACTTTACTAGCCAGCCTTTGACATCA
BPIFB2-14:
ACCTGATGCCATGCATCGCACTAACCCAATATTATTAACCGCACCACTCACATAAATCAAGCTAGCCAGCCTTTGACATCA
BPIFB2-15:
ACCTGATGCCATGCATCGCATATCAACTCTCATATATTCTTCACATCCCTACCCTTGTTACCTAGCCAGCCTTTGACATCA
BPIFB2-16:
ACCTGATGCCATGCATCGCAACAACATCTATCTACTTATCAACTTCAAAACTTTCATATTACTAGCCAGCCTTTGACATCA
实施例3蛋白结合适配子的性能测定
将适配子分别取2.0μg,用牛小肠碱性磷酸酶(CIP)37℃消化lh,纯化回收去磷酸化的RNA;通过T4多核苷酸激酶标记[γ-32P]ATP于去磷酸化的RNA分子末端。10nmol放射性标记的适配子分别与不同浓度(1-200nM)的BPIFB237℃孵育30min,各组反应液经硝酸纤维素膜滤过,洗涤滤膜,干燥滤膜,液闪计数仪测定滤膜上残留的放射量,同一样品平行做两次测定。计算各个适配子与目的蛋白的解离常数。结果如下:
实施例4所述适配子特异性分析以及稳定性分析
分别采用人血白蛋白,免疫血清球蛋白,霍乱弧菌VgrG3C蛋白,大肠杆菌外膜蛋白A,COCH蛋白,BPIFB2蛋白,与16条适配子进行特异性检测,经过结合试验发现,这些适配子都不与这些蛋白相结合,而只与BPIFB2蛋白结合保持较高的特异性。
将所述的适配子,取0.2ug,分别置于常温的血清、水溶液中,放置三周。通过RT-PCR检测,发现三周的放置其结构稳定,没有被降解。
实施例5所述适配子疾病的诊断
取11个口腔癌患者和4个正常人的唾液分泌物,使用生理盐水稀释,获得目标样本。
将16个偶联有标记的适配子分别与11个患者以及4个正常人的分泌物混合30min,通过生物素分离,定量分析其中的BPIFB2蛋白的含量,通过分析发现,11个口腔癌患者中BPIFB2蛋白的含量显著增加,超过了规定的阈值。达到了口腔癌的诊断标准。由此可见,其诊断效果较好。
以上仅为本发明的优选实施例而已,并不用于限制本发明,对于本领域的技术人员来说,凡在本发明的精神和原则之内所做的任何修改、等同替换、改进等,均应包含在本发明的保护范围之内。
序列表
〈110〉陈博
〈120〉一种口腔癌特异性检测的试剂盒
〈160〉15
〈210〉1
〈211〉 81
〈212〉DNA
〈213〉人工序列
〈400〉BPIFB2-1
ACCTGATGCCATGCATCGCATCACACGATCATCACACCGCTCCGCCTTCCAACTACCGCTC
CTAGCCAGCCTTTGACATCA
〈210〉2
〈211〉 81
〈212〉DNA
〈213〉人工序列
〈400〉BPIFB2-2
ACCTGATGCCATGCATCGCACACCTCCACCCTTCGCAACTACCTCTCTTCCTGTAACTATT
CTAGCCAGCCTTTGACATCA
〈210〉3
〈211〉 81
〈212〉DNA
〈213〉人工序列
〈400〉BPIFB2-3
ACCTGATGCCATGCATCGCATCTCATAACAAATCTTCATAATCTTCTATAACACTGTCAAT
CTAGCCAGCCTTTGACATCA
〈210〉4
〈211〉 81
〈212〉DNA
〈213〉人工序列
〈400〉BPIFB2-4
ACCTGATGCCATGCATCGCACATCTATATAGAATATATTCAATCCTCTCTCTTATATGCAT
CTAGCCAGCCTTTGACATCA
〈210〉5
〈211〉 81
〈212〉DNA
〈213〉人工序列
〈400〉BPIFB2-5
ACCTGATGCCATGCATCGCACTATCAACGACTCTCACGCAACGCTCTTCTTACTACATATA
CTAGCCAGCCTTTGACATCA
〈210〉6
〈211〉 81
〈212〉DNA
〈213〉人工序列
〈400〉BPIFB2-6
ACCTGATGCCATGCATCGCACCATTCATATCACGAACACCCATTTATACTATATACTACCA
CTAGCCAGCCTTTGACATCA
〈210〉7
〈211〉 81
〈212〉DNA
〈213〉人工序列
〈400〉BPIFB2-7
ACCTGATGCCATGCATCGCACGCACTAATATCCTTCATCCCTACTAATAACTCTTTTAACC
CTAGCCAGCCTTTGACATCA
〈210〉8
〈211〉 81
〈212〉DNA
〈213〉人工序列
〈400〉BPIFB2-8
ACCTGATGCCATGCATCGCACACACTCATATTTATACACCAATATCATCTCACTCTATCTC
CTAGCCAGCCTTTGACATCA
〈210〉9
〈211〉 81
〈212〉DNA
〈213〉人工序列
〈400〉BPIFB2-9
ACCTGATGCCATGCATCGCAAACCAAGAATGTCTTACGACATCTCGCCTACTATCCTATAT
CTAGCCAGCCTTTGACATCA
〈210〉10
〈211〉 81
〈212〉DNA
〈213〉人工序列
〈400〉BPIFB2-10
ACCTGATGCCATGCATCGCACACATAACAATCTATTATTCCTCTATCCGCTCTTATATTCC
CTAGCCAGCCTTTGACATCA
〈210〉11
〈211〉 81
〈212〉DNA
〈213〉人工序列
〈400〉BPIFB2-11
ACCTGATGCCATGCATCGCAAACCAGTCTACTCAACCGACCACCTCAATATCATTCTCATC
CTAGCCAGCCTTTGACATCA
〈210〉12
〈211〉 81
〈212〉DNA
〈213〉人工序列
〈400〉BPIFB2-12
ACCTGATGCCATGCATCGCACTACCTATACAATACTTACTGTCTGCTTCTACTTCTATTAT
CTAGCCAGCCTTTGACATCA
〈210〉13
〈211〉 81
〈212〉DNA
〈213〉人工序列
〈400〉BPIFB2-13
ACCTGATGCCATGCATCGCATATACTCGCAATCACTATATAAGACCACATAAATCACTTTA
CTAGCCAGCCTTTGACATCA
〈210〉14
〈211〉 81
〈212〉DNA
〈213〉人工序列
〈400〉BPIFB2-14
ACCTGATGCCATGCATCGCACTAACCCAATATTATTAACCGCACCACTCACATAAATCAAG
CTAGCCAGCCTTTGACATCA
〈210〉15
〈211〉 81
〈212〉DNA
〈213〉人工序列
〈400〉BPIFB2-15
ACCTGATGCCATGCATCGCATATCAACTCTCATATATTCTTCACATCCCTACCCTTGTTAC
CTAGCCAGCCTTTGACATCA
〈210〉16
〈211〉 81
〈212〉DNA
〈213〉人工序列
〈400〉BPIFB2-16
ACCTGATGCCATGCATCGCAACAACATCTATCTACTTATCAACTTCAAAACTTTCATATTA
CTAGCCAGCCTTTGACATCA
Claims (3)
1.一种用于口腔癌检测的试剂盒,其含有能特异性与BPIFB2蛋白结合的核酸适体。
2.如权利要求1所述的试剂盒,其特征在于:所述核酸适体序列为SEQ ID No:3所示。
3. 一种检测口腔癌的方法,其特征在于利用权利要求1-2任一项所述的试剂盒。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610503507.8A CN105911282A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510775735.6A CN105203763B (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610503507.8A CN105911282A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510775735.6A Division CN105203763B (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN105911282A true CN105911282A (zh) | 2016-08-31 |
Family
ID=54951548
Family Applications (16)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610503476.6A Pending CN105891513A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610505473.6A Pending CN105954520A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610504451.8A Pending CN105891515A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610505437.XA Pending CN105891518A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610503478.5A Pending CN105891486A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610503506.3A Pending CN105911295A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610505458.1A Pending CN106053814A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610505457.7A Pending CN105954519A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610504453.7A Pending CN105891517A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610505490.XA Pending CN105929163A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610504440.XA Pending CN105891514A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610503479.XA Pending CN105891487A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610504455.6A Pending CN105891488A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610504452.2A Pending CN105891516A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201510775735.6A Active CN105203763B (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610503507.8A Pending CN105911282A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
Family Applications Before (15)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610503476.6A Pending CN105891513A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610505473.6A Pending CN105954520A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610504451.8A Pending CN105891515A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610505437.XA Pending CN105891518A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610503478.5A Pending CN105891486A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610503506.3A Pending CN105911295A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610505458.1A Pending CN106053814A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610505457.7A Pending CN105954519A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610504453.7A Pending CN105891517A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610505490.XA Pending CN105929163A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610504440.XA Pending CN105891514A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610503479.XA Pending CN105891487A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610504455.6A Pending CN105891488A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201610504452.2A Pending CN105891516A (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
CN201510775735.6A Active CN105203763B (zh) | 2015-11-15 | 2015-11-15 | 一种口腔癌特异性检测的试剂盒 |
Country Status (1)
Country | Link |
---|---|
CN (16) | CN105891513A (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106018828A (zh) * | 2016-06-19 | 2016-10-12 | 曹帅 | 一种用于肠道疾病检测的试剂盒 |
CN106093434A (zh) * | 2016-06-19 | 2016-11-09 | 曹帅 | 一种用于肝癌检测的试剂盒 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011133770A2 (en) * | 2010-04-21 | 2011-10-27 | Board Of Regents Of The University Of Texas System | Salivary protein markers for detection of breast cancer |
CN104059982A (zh) * | 2014-07-04 | 2014-09-24 | 杨承刚 | 口腔癌致病基因bpifb2及其应用 |
CN104830867A (zh) * | 2015-06-07 | 2015-08-12 | 杨洋 | 一种能够特异性结合癌细胞中的dkk1蛋白的适配子 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101014609A (zh) * | 2004-04-26 | 2007-08-08 | 阿切埃米克斯有限公司 | 免疫球蛋白e的特异性核酸配体及其作为过敏性疾病治疗的应用 |
WO2007048978A2 (fr) * | 2005-10-28 | 2007-05-03 | Biomerieux Sa | Procede de detection du cancer |
US20100279419A1 (en) * | 2007-09-18 | 2010-11-04 | Board Of Regents Of The University Of Texas System | Detection of saliva proteins modulated secondary to ductal carcinoma in situ of the breast |
JP5899550B2 (ja) * | 2010-02-12 | 2016-04-06 | 株式会社リボミック | Fgf2に対するアプタマー及びその使用 |
KR102242874B1 (ko) * | 2013-03-14 | 2021-04-21 | 소마로직, 인크. | Il―6에 결합하는 압타머 및 il―6 매개된 질환을 치료하거나 진단하는 데에서 이의 용도 |
CN104267185B (zh) * | 2014-09-12 | 2016-05-18 | 范飞舟 | 检测肿瘤的试剂盒及其专用识别n-乙酰氨基葡萄糖的物质 |
CN104818278A (zh) * | 2015-04-17 | 2015-08-05 | 刘红卫 | 能够特异性结合胃癌细胞中的ts/mdep蛋白的适配子 |
-
2015
- 2015-11-15 CN CN201610503476.6A patent/CN105891513A/zh active Pending
- 2015-11-15 CN CN201610505473.6A patent/CN105954520A/zh active Pending
- 2015-11-15 CN CN201610504451.8A patent/CN105891515A/zh active Pending
- 2015-11-15 CN CN201610505437.XA patent/CN105891518A/zh active Pending
- 2015-11-15 CN CN201610503478.5A patent/CN105891486A/zh active Pending
- 2015-11-15 CN CN201610503506.3A patent/CN105911295A/zh active Pending
- 2015-11-15 CN CN201610505458.1A patent/CN106053814A/zh active Pending
- 2015-11-15 CN CN201610505457.7A patent/CN105954519A/zh active Pending
- 2015-11-15 CN CN201610504453.7A patent/CN105891517A/zh active Pending
- 2015-11-15 CN CN201610505490.XA patent/CN105929163A/zh active Pending
- 2015-11-15 CN CN201610504440.XA patent/CN105891514A/zh active Pending
- 2015-11-15 CN CN201610503479.XA patent/CN105891487A/zh active Pending
- 2015-11-15 CN CN201610504455.6A patent/CN105891488A/zh active Pending
- 2015-11-15 CN CN201610504452.2A patent/CN105891516A/zh active Pending
- 2015-11-15 CN CN201510775735.6A patent/CN105203763B/zh active Active
- 2015-11-15 CN CN201610503507.8A patent/CN105911282A/zh active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011133770A2 (en) * | 2010-04-21 | 2011-10-27 | Board Of Regents Of The University Of Texas System | Salivary protein markers for detection of breast cancer |
CN104059982A (zh) * | 2014-07-04 | 2014-09-24 | 杨承刚 | 口腔癌致病基因bpifb2及其应用 |
CN104830867A (zh) * | 2015-06-07 | 2015-08-12 | 杨洋 | 一种能够特异性结合癌细胞中的dkk1蛋白的适配子 |
Also Published As
Publication number | Publication date |
---|---|
CN105954519A (zh) | 2016-09-21 |
CN105891517A (zh) | 2016-08-24 |
CN105911295A (zh) | 2016-08-31 |
CN105203763A (zh) | 2015-12-30 |
CN105891486A (zh) | 2016-08-24 |
CN105954520A (zh) | 2016-09-21 |
CN105929163A (zh) | 2016-09-07 |
CN105891487A (zh) | 2016-08-24 |
CN105891514A (zh) | 2016-08-24 |
CN105891516A (zh) | 2016-08-24 |
CN105203763B (zh) | 2017-01-04 |
CN105891518A (zh) | 2016-08-24 |
CN105891515A (zh) | 2016-08-24 |
CN105891513A (zh) | 2016-08-24 |
CN105891488A (zh) | 2016-08-24 |
CN106053814A (zh) | 2016-10-26 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN105203763B (zh) | 一种口腔癌特异性检测的试剂盒 | |
CN105353126B (zh) | 一种唇癌特异性检测的试剂盒 | |
CN106018842A (zh) | 一种用于检测眼科疾病的试剂盒及其检测方法 | |
CN105823879A (zh) | 一种子宫内膜癌特异性检测的试剂盒 | |
CN105823894A (zh) | 一种子宫内膜癌特异性检测的试剂盒 | |
CN105823895A (zh) | 一种子宫内膜癌特异性检测的试剂盒 | |
CN105785007A (zh) | 一种子宫内膜癌特异性检测的试剂盒 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160831 |
|
WD01 | Invention patent application deemed withdrawn after publication |