CN105910847A - Lyophobic micro-valve type trace liquid extraction apparatus and method with adjustable liquid extraction volume - Google Patents
Lyophobic micro-valve type trace liquid extraction apparatus and method with adjustable liquid extraction volume Download PDFInfo
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- CN105910847A CN105910847A CN201610203354.5A CN201610203354A CN105910847A CN 105910847 A CN105910847 A CN 105910847A CN 201610203354 A CN201610203354 A CN 201610203354A CN 105910847 A CN105910847 A CN 105910847A
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- lyophoby
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- liquid extraction
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- 239000007788 liquid Substances 0.000 title claims abstract description 118
- 238000000605 extraction Methods 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 26
- 239000012530 fluid Substances 0.000 claims abstract description 16
- 239000011159 matrix material Substances 0.000 claims abstract description 11
- 239000007921 spray Substances 0.000 claims description 11
- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 10
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 10
- 230000008569 process Effects 0.000 claims description 9
- 238000001259 photo etching Methods 0.000 claims description 7
- -1 polydimethylsiloxane Polymers 0.000 claims description 5
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 4
- 238000001338 self-assembly Methods 0.000 claims description 4
- 229910052710 silicon Inorganic materials 0.000 claims description 4
- 239000010703 silicon Substances 0.000 claims description 4
- 239000002356 single layer Substances 0.000 claims description 4
- 239000010409 thin film Substances 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 2
- 150000004702 methyl esters Chemical class 0.000 claims description 2
- 238000000465 moulding Methods 0.000 claims description 2
- 239000000284 extract Substances 0.000 abstract description 4
- 230000000694 effects Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000000903 blocking effect Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 2
- 239000004926 polymethyl methacrylate Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229920002120 photoresistant polymer Polymers 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- ZDHXKXAHOVTTAH-UHFFFAOYSA-N trichlorosilane Chemical compound Cl[SiH](Cl)Cl ZDHXKXAHOVTTAH-UHFFFAOYSA-N 0.000 description 1
- 239000005052 trichlorosilane Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
Abstract
The invention discloses a lyophobic micro-valve type trace liquid extraction apparatus and method with an adjustable liquid extraction volume. The apparatus comprises a matrix and a fluid inlet, an air pressure inlet, lyophobic micro-valves, a capillary channel and a nozzle formed on the matrix, wherein the capillary channel comprises a main path forming a loop and a plurality of branch paths arranged between and connected with the main path and the nozzle in a parallel manner, the fluid inlet and the air pressure inlet are connected with the main path, each branch path is provided with one lyophobic micro-valve having a set pressure resistant value and operating on the basis of lyophobic principles, and outlets of all the branch paths are connected with the nozzle. The lyophobic micro-valve type trace liquid extraction apparatus and method provided by the invention can conveniently, flexibly and accurately extract liquid with a volume of dozens of picoliters to dozens of nano-liters.
Description
Technical field
The present invention relates to microfluidic control field, take the adjustable lyophoby of liquid measure more specifically to one
Micro-valve type micro liquid extraction element and method.
Background technology
Micro-fluidic technologies refers to the technology that convection cell is controlled, operates and detects under micro-scale.
Along with the development in the fields such as biological detection, pharmacopedics, environmental science, need the amount of liquid extracted continuous
Reduce, in protein crystallization experiments, it is only necessary to the liquid of tens nanoliters, in DNA, gene studies,
The least to the hundreds of picoliters of amount of liquid.It is contemplated that, in following research, the amount of liquid will enter one
Step reduces, and the performance accuracy of microfluid control device is had higher requirement by this.
Microfluid control device includes Micropump, micro-valve, micro-mixer, mini sprinkler etc., for trace
Fluid is driven, drain, mix, extract, distribute, the operation such as blocking-up.The most micro-valve is to play
The important devices of the restriction effect of liquid in runner, micro-valve can be divided into active micro valve and Passive microvalve two class,
Differring primarily in that whether valve arrangement itself has actuator, aggressive valve is that the motion by actuator realizes
The opening and closing of micro-valve, passive valve does not has actuator, and the change by the parameter of fluid own realizes opening and closing.Passively
The advantage of valve be embodied in simple in construction, easily process, take up room little, compatible good, it is adaptable to simple
The microfluidic valve control of occasion.Utilize the restriction effect to liquid stream of micro-valve, the isolation of micro liquid can be realized
And extraction, designed by the structure of micro-valve, be accurately controlled the volume of extracting liq.Liquid releasing valve by
The one of dynamic micro-valve, by reducing the surface energy in valve district, utilizes the surface tension of liquid to block liquid stream
Dynamic, increase fluid pressure and valve can be made to open.
Summary of the invention
Present invention is primarily targeted at and overcome the deficiencies in the prior art, solve existing liquid extraction apparatus
The problem lacking picoliters level liquid handling capacity, it is provided that one takes the adjustable lyophoby of liquid measure micro-valve type trace
Liquid extraction apparatus and method, can facilitate, process tens picoliters flexibly, accurately to tens nanoliters of bodies
Long-pending liquid extraction.
For achieving the above object, the present invention is by the following technical solutions:
One takes the adjustable lyophoby of liquid measure micro-valve type micro liquid extraction element, including matrix and being formed at
The micro-valve of fluid intake on described matrix, air pressure inlet, lyophoby, capillary runner and nozzle, described hair
Thread road includes the main line being formed as a loop and is connected in parallel between described main line and described nozzle
Multiple branch roads, described fluid intake and described air pressure inlet connect described main line, each branch road set
Having the micro-valve of lyophoby having and setting pressure voltage based on lyophoby principle, the outlet of each branch road connects described
Nozzle.
Further:
At least partly the pressure voltage of the micro-valve of lyophoby on branch road is different.
The plurality of branch road intersects vertically with described main line abreast.
Preferably, described main line is configured with two half round runner as opposed end, by two
Bar line flowing channel is connected and constitutes loop, and the plurality of branch road is connected to wherein on straight line runner.
Described liquid inlet, air pressure inlet, capillary runner and nozzle are prepared by photoetching or hot press method,
It is preferably MEMS photoetching process to prepare.
The degree of depth of described capillary runner is 10～50 microns, and width is 50～500 microns.
The micro-valve of described lyophoby uses monolayer self-assembly method to prepare.
The contact angle of the micro-valve of described lyophoby and institute's extracting liq is more than 100 °.
Above described capillary runner with polydimethylsiloxane (PDMS), polymethyl methacrylate or
Silicon thin film is bonded to form closed flow.
Also including that scalable drives the actuating device of atmospheric pressure of air pressure, described actuating device of atmospheric pressure is connected to institute
State air pressure inlet, be used for driving fluid to flow in described capillary runner.
One takes the adjustable lyophoby of liquid measure micro-valve type micro liquid extracting method, uses described lyophoby micro-
Valve type micro liquid extraction element, comprises the following steps:
Drive a certain amount of liquid by described stream with the air pressure opening pressure less than the micro-valve of all lyophobies
Body entrance enters described capillary runner, the intersection point shunting between described main line and each branch road, successively
Fill up each intersection point to the branch flow channel between the micro-valve of each lyophoby, remaining liquid along loop by air pressure push back institute
State at liquid inlet；
The amount of liquid extracted as required, the pressure voltage of the micro-valve of each lyophoby and each intersection point are micro-to each lyophoby
The liquid capacity of the branch flow channel between valve, increases and drives air pressure, makes pressure voltage be less than and drives dredging of air pressure
The micro-valve of liquid is open, in the temporary liquid towards spout of corresponding branch road and spray.
Beneficial effects of the present invention:
The present invention by the blocking action of the micro-valve of lyophoby realize liquid spray before prefilled, by lyophoby
The temporary liquid of filling of micro-valve, then make liquid overcome the obstruction of the micro-valve of lyophoby to make by the way of increasing pressure
With, nozzle spray, thus accurately extract the micro-valve of lyophoby to the trace liquid in runner between split point
Body.Due to liquid loading by capillary runner branch road size and participate in liquid extraction circuitry number
Determine, can facilitate, process the liquid that volume is tens picoliters to tens nanoliters flexibly, accurately.
It is an advantage of the current invention that admission space accurately can be controlled by the size of design capillary runner,
Designed the on-demand opening realizing the micro-valve of lyophoby by the differentiation of valve pressure voltage micro-to lyophoby, thus reach
The purpose that volume is controlled, and unnecessary liquid may return to liquid inlet recycling, easily realize skin
The fine droplet of upgrading, and control mode is simple.
The present invention realization that combined with micro-spray by micro-valve takes liquid measure adjustable picoliters level liquid extraction, structure
Simply, easy and simple to handle, compatible good, the liquid that can be widely used for microfluidic control field accurately extracts
Operation.
Accompanying drawing explanation
Fig. 1 is lyophoby micro-valve type micro liquid extraction element structural representation of the embodiment of the present invention.
Fig. 2 is a specific embodiment of micro liquid extraction element of the present invention.
Fig. 3 a to Fig. 3 e is lyophoby micro-valve type micro liquid extracting method extracting solution of the embodiment of the present invention
The process schematic of body.
Description of reference numerals:
1-liquid inlet and the liquid entered here,
2-air pressure inlet and the gas entered here,
3-capillary runner,
The micro-valve of 4-lyophoby,
5-nozzle,
6-branch road,
7-matrix.
Detailed description of the invention
Hereinafter embodiments of the present invention are elaborated.It is emphasized that the description below is only
It is exemplary rather than in order to limit the scope of the present invention and application thereof.
Refering to Fig. 1 to Fig. 3 e, in one embodiment, to take the liquid measure micro-valve type of adjustable lyophoby micro-for one
Quantity of fluid extraction element, the fluid intake 1 including matrix 7 and being formed on described matrix 7, air pressure
The micro-valve of entrance 2, lyophoby 4, capillary runner and nozzle 5, described capillary runner includes being formed as a ring
The main line 3 on road and the multiple branch roads 6 being connected in parallel between described main line 3 and described nozzle 5, institute
State fluid intake 1 and described air pressure inlet 2 connect described main line 3, each branch road 6 be provided with based on
The micro-valve of lyophoby 4 with setting pressure voltage of lyophoby principle, the outlet of each branch road 6 connects described spray
Mouth 5.Matrix 7 can use silicon, photoresist, polydimethylsiloxane (PDMS) or polymethyl
The materials such as acid methyl ester (PMMA) are made.
During work, liquid enters capillary runner from fluid intake 1, under the effect of the pressure in capillary flow
Flow in road, and liquid inlet can be returned to, after liquid enters branch road 6, at the micro-valve of lyophoby 4 due to
Lyophoby effect, flows hindered, and a part of liquid is temporarily stored in the liquid in branch road 6, in main line 3
Being pushed back liquid inlet by pressure, improve the size driving pressure the most as required, all pressure voltages are little
Open in the micro-valve of lyophoby 4 driving pressure, the liquid ejection that place branch road 6 is temporary, big by pressure
The number that the little micro-valve of control lyophoby 4 is opened, thus control the volume of extracting liq.
In certain embodiments, the pressure voltage of the micro-valve of lyophoby 4 on each branch road 6 is different.When
So, it is also possible to the pressure voltage being designed to partial branch 6 is identical.Owing to each branch road 6 can keep in one
Quantitative liquid, and lyophoby micro-valve 4 pressure voltage of each branch road 6 is different, drives pressure to exceed accordingly
During the pressure voltage of branch road 6, liquid temporary in respective branch 6 will be by the micro-valve of lyophoby 4, therefore,
By regulating the driving pressure of air pressure inlet 2, the micro liquid that volume classification is variable can be extracted.
In a preferred embodiment, the plurality of branch road 6 intersects vertically with described main line 3 abreast.
Especially, main line 3 can be configured with two half round runner as opposed end, by two
Line flowing channel is connected and constitutes loop, and multiple branch roads 6 are connected to wherein on straight line runner.
Described liquid inlet, air pressure inlet 2, capillary runner and nozzle 5 can be by photoetching or hot mouldings
Prepared by technique, as prepared by MEMS photoetching process.
In a preferred embodiment, the degree of depth of described capillary runner is 10～50 microns, and width is 50～500
Micron.
The micro-valve of described lyophoby 4 can use monolayer self-assembly method to prepare.
In a preferred embodiment, the contact angle of the micro-valve of described lyophoby 4 and institute's extracting liq 100 ° with
On.
The available above polydimethylsiloxane of described capillary runner (PDMS), polymethyl methacrylate,
Or silicon thin film is bonded to form closed flow.
It is a specific embodiment of lyophoby of the present invention micro-valve type micro liquid extraction element shown in Fig. 2,
Wherein prepared by capillary runner SU-8 photoetching process, the micro-valve of lyophoby 4 by 1H, 1H, 2H, 2H-perfluor last of the ten Heavenly stems
Prepared by base trichlorosilane monolayer self-assembly process, with polydimethylsiloxane (PDMS) above runner
Thin film is bonded, with closed flow.The present embodiment has 10 branch roads 6, the capillary flow of each branch road 6
Road is wide 50 microns, deep 10 microns, and the distance of the micro-valve of intersection point to lyophoby 4 is 50 microns, therefore can carry
Take the liquid of 25～250 picoliters.
In further embodiments, a kind of lyophoby micro-valve type micro liquid extraction element, before including
State outside the matrix 7 of any embodiment, also include that scalable drives the actuating device of atmospheric pressure (figure of air pressure
Do not show), described actuating device of atmospheric pressure is connected to described air pressure inlet 2, is used for driving fluid at described hair
Thread road flows.
Refering to Fig. 3 a to Fig. 3 e, in one embodiment, one takes the liquid measure micro-valve type of adjustable lyophoby
Micro liquid extracting method, uses described lyophoby micro-valve type micro liquid extraction element, including following
Step:
Drive a certain amount of liquid by described stream with the air pressure opening pressure less than the micro-valve of all lyophobies 4
Body entrance 1 enters described capillary runner, the intersection point shunting between described main line 3 and each branch road 6,
Fill up each intersection point successively to branch road 6 runner between the micro-valve of each lyophoby 4, remaining liquid along loop by gas
Pressure pushes back at described liquid inlet；
The amount of liquid extracted as required, the pressure voltage of the micro-valve of each lyophoby 4 and each intersection point are to each lyophoby
The liquid capacity of branch road 6 runner between micro-valve 4, increases and drives air pressure, make pressure voltage be less than driving gas
Lyophoby micro-valve 4 of pressure is open, in the temporary liquid towards spout 5 of corresponding branch road 6 and spray.
Refering to Fig. 1 to Fig. 3 e, according to embodiments of the invention, micro-valve is combined with micro-spray, by dredging
It is prefilled that the blocking action of the micro-valve of liquid 4 realizes before liquid ejection, then by increasing by the way of pressure gram
Take the blocking action of the micro-valve of lyophoby 4, nozzle 5 spray, lyophoby micro-valve 4 pressure voltage of each branch road 6
It is different from, therefore can control the quantity that lyophoby micro-valve 4 is open by the way of regulation drives pressure,
Amount of liquid needed for grading extraction.The size of capillary runner determines the loadings of each branch road 6, and connects
Connecing liquid inlet, air pressure inlet 2 and nozzle 5, the micro-valve of lyophoby 4 is arranged on branch road 6, all
Road 6 is converged at nozzle 5, and between micro-valve to main line 3 and branch road 6 intersection point, the space in runner is for being somebody's turn to do
The extractible amount of liquid of branch road 6.During extracting liq, a certain amount of liquid is less than the micro-valve of lyophoby at one
The air pressure of 4 pressure voltages is entered capillary runner by liquid inlet under driving, and shunts in point of intersection, fills out successively
Full each intersection point is to branch road 6 runner between micro-valve, and remaining liquid is pushed back liquid inlet by pressure, now
On-demand increase drives pressure, makes pressure open less than the lyophoby micro-valve 4 driving pressure, place branch road 6
Temporary liquid towards spout 5 also sprays, and completes the extraction of a liquid.
Refering to the micro-valve type micro liquid extracting method of the lyophoby shown in Fig. 3 a to Fig. 3 e, liquid is by liquid
Entrance enters capillary runner, and gas is entered by air pressure inlet 2, and promotes liquid to arrive the micro-valve of lyophoby 4,
This drives pressure should be less than the open pressure of the micro-valve of all lyophobies 4, owing to the micro-valve of lyophoby 4 hinders liquid
The flowing of body, liquid flows at capillary runner indraft body entrance 1, and each branch road 6 only has a little portion
Separatory body is stayed in the capillary runner between Heavenly Stems and Earthly Branches road intersection point and the micro-valve of lyophoby 4, now increases driving pressure
Power, partially liq overcomes the obstruction of the micro-valve of lyophoby 4, converges to nozzle 5 and sprays, a micro liquid
Extraction completes.
Above content is that combination is concrete/the most made for the present invention the most specifically
Bright, it is impossible to assert the present invention be embodied as be confined to these explanations.For technology belonging to the present invention
For the those of ordinary skill in field, without departing from the inventive concept of the premise, it can also be to this
The embodiment having described that a bit makes some replacements or modification, and these substitute or variant all should
It is considered as belonging to protection scope of the present invention.
Claims (10)
1. one kind takes the adjustable lyophoby of liquid measure micro-valve type micro liquid extraction element, it is characterised in that
Including matrix and the fluid intake formed on the matrix, air pressure inlet, the micro-valve of lyophoby, capillary flow
Road and nozzle, described capillary runner includes the main line being formed as a loop and is connected in parallel described dry
Multiple branch roads between road and described nozzle, described fluid intake and described air pressure inlet connect described dry
Road, each branch road is provided with the micro-valve of lyophoby having and setting pressure voltage based on lyophoby principle, each
The outlet on road connects described nozzle.
2. lyophoby micro-valve type micro liquid extraction element as claimed in claim 1, it is characterised in that
At least partly the pressure voltage of the micro-valve of lyophoby on branch road is different.
3. lyophoby micro-valve type micro liquid extraction element as claimed in claim 1, it is characterised in that
The plurality of branch road intersects vertically with described main line abreast；Preferably, described main line is configured with
Two half round runner, as opposed end, are connected by two line flowing channels and constitute loop, described
Multiple branch roads are connected to wherein on straight line runner.
4. the lyophoby micro-valve type micro liquid extraction element as described in any one of claims 1 to 3,
It is characterized in that, described liquid inlet, air pressure inlet, capillary runner and nozzle are by photoetching or hot moulding
Prepared by technique, prepared by preferably MEMS photoetching process.
5. the lyophoby micro-valve type micro liquid extraction element as described in any one of claims 1 to 3,
It is characterized in that, the degree of depth of described capillary runner is 10～50 microns, and width is 50～500 microns.
6. the lyophoby micro-valve type micro liquid extraction element as described in any one of claims 1 to 3,
It is characterized in that, the micro-valve of described lyophoby uses monolayer self-assembly method to prepare.
7. lyophoby micro-valve type micro liquid extraction element as claimed in claim 5, it is characterised in that
The contact angle of the micro-valve of described lyophoby and institute's extracting liq is more than 100 °.
8. the lyophoby micro-valve type micro liquid extraction element as described in any one of claims 1 to 3,
It is characterized in that, with polydimethylsiloxane (PDMS), polymethyl above described capillary runner
Acid methyl ester or silicon thin film are bonded to form closed flow.
9. the lyophoby micro-valve type micro liquid extraction element as described in any one of claim 1 to 8,
It is characterized in that, also include that scalable drives the actuating device of atmospheric pressure of air pressure, described actuating device of atmospheric pressure
It is connected to described air pressure inlet, is used for driving fluid to flow in described capillary runner.
10. one kind takes the adjustable lyophoby of liquid measure micro-valve type micro liquid extracting method, it is characterised in that
Use the lyophoby micro-valve type micro liquid extraction element as described in any one of claim 1 to 9, including
Following steps:
Drive a certain amount of liquid by described stream with the air pressure opening pressure less than the micro-valve of all lyophobies
Body entrance enters described capillary runner, the intersection point shunting between described main line and each branch road, successively
Fill up each intersection point to the branch flow channel between the micro-valve of each lyophoby, remaining liquid along loop by air pressure push back institute
State at liquid inlet；
The amount of liquid extracted as required, the pressure voltage of the micro-valve of each lyophoby and each intersection point are micro-to each lyophoby
The liquid capacity of the branch flow channel between valve, increases and drives air pressure, makes pressure voltage be less than and drives dredging of air pressure
The micro-valve of liquid is open, in the temporary liquid towards spout of corresponding branch road and spray.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610203354.5A CN105910847B (en) | 2016-04-01 | 2016-04-01 | A kind of adjustable lyophoby micro-valve type micro liquid extraction apparatus of liquid-taken amount and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610203354.5A CN105910847B (en) | 2016-04-01 | 2016-04-01 | A kind of adjustable lyophoby micro-valve type micro liquid extraction apparatus of liquid-taken amount and method |
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| Publication Number | Publication Date |
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| CN105910847A true CN105910847A (en) | 2016-08-31 |
| CN105910847B CN105910847B (en) | 2018-08-07 |
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| CN201610203354.5A Expired - Fee Related CN105910847B (en) | 2016-04-01 | 2016-04-01 | A kind of adjustable lyophoby micro-valve type micro liquid extraction apparatus of liquid-taken amount and method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108339580A (en) * | 2018-03-20 | 2018-07-31 | 哈尔滨工业大学深圳研究生院 | A kind of fluid shearing force generating mechanism and hydrodynamic shear generation method |
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| CN105278587A (en) * | 2015-10-26 | 2016-01-27 | 哈尔滨工业大学 | Multi-channel microfluidic pressure source |
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| CN2625577Y (en) * | 2003-06-06 | 2004-07-14 | 中国石化胜利油田有限公司采油工艺研究院 | Automatic dispensing apparatus for microflow liquid |
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| CN104571152A (en) * | 2015-01-20 | 2015-04-29 | 哈尔滨工业大学 | Liquid drop micro fluid control closed-loop regulation device based on microvalve |
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| CN105910847B (en) | 2018-08-07 |
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