CN105886389A - Culture flask for detecting microorganism in human blood or human body fluid and preparation method of culture flask - Google Patents
Culture flask for detecting microorganism in human blood or human body fluid and preparation method of culture flask Download PDFInfo
- Publication number
- CN105886389A CN105886389A CN201610480046.7A CN201610480046A CN105886389A CN 105886389 A CN105886389 A CN 105886389A CN 201610480046 A CN201610480046 A CN 201610480046A CN 105886389 A CN105886389 A CN 105886389A
- Authority
- CN
- China
- Prior art keywords
- bottle
- flask
- culture
- body fluid
- culture flask
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention aims to provide a culture flask being complete in components, accurate and efficient to detect, and capable of automatically detecting microorganism in human blood or human body fluid, and a preparation method of the culture flask. The culture flask comprises a flask body (1), wherein a pressure sensor is arranged at the bottom part of the flask body (1); a thin film is arranged between the pressure sensor and an inner chamber of the flask body (1); the components in the flask body (1), according to 1000 parts by weight, comprise 10 parts of yeast extract powder, 20 parts of brain heart infusion, 1 part of sodium bicarbonate, 0.5 part of sodium polyanethole sulfonate, 0.02 part of menadione, 0.01 part of hemin, and the balance of purified water. The method comprises the following steps: sterilizing the flask body, and dissolving all raw materials fed into the flask body; performing sterile filling for the flask body, and replacing air in the flask by an air pumping and exchanging manner; sealing the flask body to obtain the culture flask for detecting the microorganism in sterile human body fluid. The culture flask and the preparation method are applicable to the technical field of biochemical detection.
Description
Technical field
The present invention relates to a kind of biochemistry detection technical field, particularly relate to a kind of detect the culture bottle of microorganism in blood of human body or body fluid, and the preparation method of this culture bottle.
Background technology
When there is various symptom in patient, various bacteria sexually transmitted disease, pneumonia, meningitis, urinary system infection etc., all may be attended by antibacterial and enter in the body fluid such as blood or hydrothorax, ascites, cerebrospinal fluid.As antibacterial enters in blood or body fluid, often produce serious consequence.When patient to the medical institutions such as hospital checks, need blood or various body fluid such as hydrothorax, ascites, cerebrospinal fluid etc. are detected, record the situation of patient body as early as possible, it is to avoid the state of an illness deteriorates further.But the quality of culture bottle conventional in the market is uneven, and quality differs, and as used ropy culture bottle, missing inspection the most easily occurs, and can reduce the positive rate of antibacterial, it is also possible to occur that " false positive " is sent out and cause mistaken diagnosis.The most not only can extend the treatment time of patient, increase medical expense, result even in serious malpractice.
Summary of the invention
The technical problem to be solved is to overcome the deficiencies in the prior art, it is provided that the culture bottle of microorganism in a kind of composition is complete, detection precise and high efficiency and can realizing detects automatically blood of human body or body fluid.
Present invention also offers the preparation method of a kind of above-mentioned culture bottle, the method is simple to operate, and the culture bottle quality prepared is good, and detection efficiency is high.
Of the present invention a kind of detect the culture bottle of microorganism in blood of human body or body fluid and be the technical scheme is that this culture bottle includes bottle, the bottom of described bottle is provided with pressure transducer, it is provided with thin film between the inner chamber of described pressure transducer and described bottle, based on 1000 weight portions, in described bottle, it is configured with following component: yeast extract powder 10 parts；Brain heart infusion 20 parts；Sodium bicarbonate 1 part；Sodium polyanethole sulfonate 0.5 part；Menadione 0.02 part；Hemin 0.01 part；Surplus is purified water.
Such scheme is visible, this culture bottle arranges pressure transducer at bottle body bottom, when adding blood or body fluid after cultivating, as produced carbon dioxide, then the pressure in bottle will change, and is changed by the pressure in the pressure sensor senses of setting to bottle, thus transfers information on the main frame of periphery, the most i.e. understanding whether there are antibacterial in blood or body fluid sample, this can realize automatically detecting；Nutritional solution in bottle includes yeast extract powder, brain heart infusion, sodium bicarbonate, sodium polyanethole sulfonate, menadione and hemin etc., its composition is complete, nutritious, detection precise and high efficiency, can realize the fast-growth of antibacterial, it is possible to be greatly shortened the discovery time of positive bacteria, more particularly there is communicable pathogen, shorten detection time, strive for the treatment time of preciousness for patient, also avoid blood not detect antibacterial and carry out blood transfusion and cause further infection.
The preparation method step of above-mentioned culture bottle is as follows:
(1) to described bottle and add the various material dissolutions in bottle and carry out degerming；
(2) sterile filling is used to take out scavenging way carry out gas displacement in bottle to described bottle, employing；
(3) sealed bottle body, obtains detecting the culture bottle of microorganism in blood of human body or body fluid.
Said method is simple to operate, and composition is complete, can realize fast culture, and culture efficiency is high, and the setting of pressure transducer, provides the foundation for follow-up detection automatically, improves automaticity.
Accompanying drawing explanation
Fig. 1 is the easy structure schematic diagram of described bottle.
Detailed description of the invention
As shown in Figure 1, in the present embodiment, culture bottle of the present invention includes bottle 1, the bottom of described bottle 1 is provided with pressure transducer, it is provided with thin film between the inner chamber of described pressure transducer and described bottle 1, based on 1000 weight portions, in described bottle 1, it is configured with following component: yeast extract powder 10 parts；Brain heart infusion 20 parts；Sodium bicarbonate 1 part；Sodium polyanethole sulfonate 0.5 part；Menadione 0.02 part；Hemin 0.01 part；Surplus is purified water.
The preparation method of above-mentioned culture bottle is as follows:
(1) to described bottle and add the various material dissolutions in bottle and carry out degerming；
(2) sterile filling is used to take out scavenging way carry out gas displacement in bottle to described bottle, employing；
(3) sealed bottle body, obtains detecting the culture bottle of sterile body fluid microorganism.
In the present invention, bottle specification can have 40mL, 30mL or 20mL.When culture bottle has growth of microorganism it is, when producing metabolite carbon dioxide, so that the pressure of culture bottle changes, pressure transducer passes the signal to the cultivation instrument of periphery, knowing cultivation positive or negative result according to signal, medical worker carries out next step treatment according to this result.
Present invention can apply to biochemistry detection technical field.
Claims (2)
1. one kind is detected the culture bottle of microorganism in blood of human body or body fluid, including bottle (1), it is characterized in that, the bottom of described bottle (1) is provided with pressure transducer, it is provided with thin film between the inner chamber of described pressure transducer and described bottle (1), based on 1000 weight portions, in described bottle (1), it is configured with following component:
Yeast extract powder 10 parts；Brain heart infusion 20 parts；Sodium bicarbonate 1 part；
Sodium polyanethole sulfonate 0.5 part；Menadione 0.02 part；
Hemin 0.01 part；Surplus is purified water.
2. the preparation method of the culture bottle of microorganism in a detection blood of human body as claimed in claim 1 or body fluid, it is characterised in that the method comprises the following steps:
(1) to described bottle and add the various material dissolutions in bottle and carry out degerming；
(2) sterile filling is used to take out scavenging way carry out gas displacement in bottle to described bottle, employing；
(3) sealed bottle body, obtains detecting the culture bottle of sterile body fluid microorganism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610480046.7A CN105886389A (en) | 2016-06-28 | 2016-06-28 | Culture flask for detecting microorganism in human blood or human body fluid and preparation method of culture flask |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610480046.7A CN105886389A (en) | 2016-06-28 | 2016-06-28 | Culture flask for detecting microorganism in human blood or human body fluid and preparation method of culture flask |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105886389A true CN105886389A (en) | 2016-08-24 |
Family
ID=56718239
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610480046.7A Pending CN105886389A (en) | 2016-06-28 | 2016-06-28 | Culture flask for detecting microorganism in human blood or human body fluid and preparation method of culture flask |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105886389A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107084907A (en) * | 2017-06-07 | 2017-08-22 | 浙江师范大学 | Blood Bacteria Culture detection method and its application |
| CN110885873A (en) * | 2019-10-22 | 2020-03-17 | 中秀科技股份有限公司 | Reagent for blood culture bottle production and blood culture bottle production process |
-
2016
- 2016-06-28 CN CN201610480046.7A patent/CN105886389A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107084907A (en) * | 2017-06-07 | 2017-08-22 | 浙江师范大学 | Blood Bacteria Culture detection method and its application |
| CN110885873A (en) * | 2019-10-22 | 2020-03-17 | 中秀科技股份有限公司 | Reagent for blood culture bottle production and blood culture bottle production process |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107779494B (en) | Kit for detecting infectious vaginitis and preparation method thereof | |
| CN105886389A (en) | Culture flask for detecting microorganism in human blood or human body fluid and preparation method of culture flask | |
| CN104450860A (en) | Pneumonia mycoplasma medium | |
| CN209178401U (en) | A kind of culture bottle examined fastly suitable for medical device sterile | |
| CN108753596B (en) | Microchamber microfluidic system for detecting microorganism growth images | |
| Ma et al. | Rapid detection of the activity of Lacticaseibacillus casei Zhang by Flow Cytometry | |
| TWI530563B (en) | Detection of bacteria in biological fluids | |
| CN103808628A (en) | Blood-culture carbon dioxide trace-gas detection system and detection method thereof | |
| CN112680499B (en) | In-vitro detection kit for anaerobic microorganisms | |
| CN107699480A (en) | A kind of blood culture detection report male device | |
| CN106350472B (en) | Liquid culture medium for culturing mycoplasma bovis and preparation method thereof | |
| CN103409495B (en) | Preparation method of saprophytic staphylococcus selective medium | |
| CN205443285U (en) | Body fluid blake bottle | |
| CN104878070A (en) | Anaerobic culture medium for culturing fusobacterium necrophorum | |
| CN207793267U (en) | A kind of blood culture detection report male device | |
| CN206089689U (en) | Cerebrospinal fluid blake bottle | |
| CN204389433U (en) | A kind of clinical medicine detects the equipment of flora fast | |
| CN106950202B (en) | Antioxidant drug screening kit | |
| CN204690002U (en) | Pressure test culturing bottle | |
| CN203117153U (en) | Biosensor for quickly detecting residual amount of tetracycline and hydroquinone | |
| CN106399207A (en) | Cow phytoplasma substrate | |
| CN203346391U (en) | Handmade blood culture bottle | |
| CN201678683U (en) | Candida fast-identification culture drug susceptibility kit | |
| CN104651528A (en) | Efficient detecting method for non-O157 shiga toxin-producing Escherichia coli | |
| CN104894214A (en) | Fusobacterium necrophorum selective medium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160824 |
|
| WD01 | Invention patent application deemed withdrawn after publication |