CN107084907A - Blood Bacteria Culture detection method and its application - Google Patents
Blood Bacteria Culture detection method and its application Download PDFInfo
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- CN107084907A CN107084907A CN201710423777.2A CN201710423777A CN107084907A CN 107084907 A CN107084907 A CN 107084907A CN 201710423777 A CN201710423777 A CN 201710423777A CN 107084907 A CN107084907 A CN 107084907A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N7/00—Analysing materials by measuring the pressure or volume of a gas or vapour
Abstract
The invention provides a kind of blood Bacteria Culture detection method and its application, it is related to blood Bacteria Detection technical field, by determining whether the gas pressure intensity being contained with the flashing lightning magnetic field detector of blood and culture medium changes, judge to whether there is bacterium in blood, alleviate the presence that existing non-invasive blood Bacteria Culture detection method detects carbon dioxide using indirect mode, cause detecting step numerous and diverse, testing cost is high, the increase of error source, as a result the technical problem of poor specificity, other materials need not only be added in flashing lightning magnetic field detector by having reached, reduce error source, simplify detecting step simultaneously, reduce testing cost, it ensure that the specificity of testing result, the technique effect of the accuracy of detection efficiency and testing result can be significantly improved.
Description
Technical field
The present invention relates to blood Bacteria Detection technical field, more particularly, to a kind of blood Bacteria Culture detection method and its
Using.
Background technology
Septicemia refers to that pathogenic bacteria invade blood circulation, and growth and breeding in blood, and what is produced toxin and trigger is acute
Systemic infection.Clinically, septicemia belongs to the critical stage of infectious diseases.Therefore, how fast and accurately to blood
Carry out Bacteria Detection significant for clinical rescue.
The method of current blood culture detection is divided into two kinds of intrusive mood and non-intrusion type, because intrusive mood detection method is to operation
Person's professional technique requires high, workload is big, time-consuming, sampled point is few, is easily caused miscellaneous bacteria interference, influences the efficiency of testing result
And specificity, cause its application to be restricted；Non-invasive inspection methods are to be metabolized generation dioxy in blood using bacterium
Change carbon as there is abacterial basis for estimation, conventional detection method has homogeneous Fluorescence Increasing detection method, color developing detection method
(carbon dioxide receptor) and radio-labeled detection method etc., these detection methods utilize carbon dioxide and fluorescent material or aobvious
The interaction of color substance is marked, the change in concentration of indirect detection carbon dioxide, and then judges in blood with the presence or absence of thin
Bacterium, however these using the detection method of fluorescence, colour developing or radio-labeled due to being to detect titanium dioxide using indirect mode
The change in concentration of carbon, causes detecting step is numerous and diverse, testing cost is high, error is originated to increase, poor specificity as a result.
In view of this, it is special to propose the present invention.
The content of the invention
An object of the present invention is to provide a kind of blood Bacteria Culture detection method, to solve existing non-intrusion type
Blood Bacteria Culture detection method detects the change in concentration of carbon dioxide using indirect mode, causes that detecting step is numerous and diverse, inspection
Survey cost height, the increase of error source, the technical problem of poor specificity as a result.
The blood Bacteria Culture detection method that the present invention is provided, by determining whether the gas pressure intensity in flashing lightning magnetic field detector is sent out
Changing, judges to whether there is bacterium in blood, is wherein contained with blood and culture medium in flashing lightning magnetic field detector.
Further, the blood Bacteria Culture detection method comprises the following steps：
(a) blood injection is contained with the flashing lightning magnetic field detector of culture medium and be well mixed, determined immediately in flashing lightning magnetic field detector
The initial gas pressure P of the gas compartment0；
(b) blood Bacteria Culture is carried out, and continues to determine real-time gas pressure P in flashing lightning magnetic field detectort, work as Pt﹥ P0When, then
Judge there is bacterium in blood.
Further, the top of the flashing lightning magnetic field detector has retained the gas compartment, and the gas compartment accounts for flashing lightning magnetic field detector
The 1/3-1/2 of volume, preferably 1/3.
Further, in step (a), the initial gas pressure P of the gas compartment in flashing lightning magnetic field detector is determined0, including such as
Lower step：
(s) using the gas compartment on laser illumination flashing lightning magnetic field detector top；
(m) laser intensity through the gas compartment is detected by detector；
(n) laser intensity is subjected to data conversion, obtains the initial gas pressure P of the gas compartment0。
Further, in step (n), first by laser intensity by data conversion into carbon dioxide absorption spectral line line
Width, then the line width of carbon dioxide absorption spectral line is converted to the initial pressure P of the gas compartment by data0。
Further, in step (b), P is worked ast﹥ P0During+δ, judge there is bacterium in blood, wherein δ is measurement variance.
Further, in step (b), the temperature for carrying out blood culture is 35-40 DEG C, preferably 37 DEG C.
Further, in step (b), real-time gas pressure PtTest period be 1-20min/ times, preferably 2-
5min/ times.
The second object of the present invention is to provide a kind of application of blood Bacteria Culture detection method, existing non-to solve
Intrusive mood blood Bacteria Culture detection method detects the change in concentration of carbon dioxide using indirect mode, causes detecting step numerous
Miscellaneous, testing cost is high, the increase of error source, the technical problem of poor specificity as a result.
The application for the blood Bacteria Culture detection method that the present invention is provided, for determine in blood with the presence or absence of anaerobic bacteria,
Aerobic bacteria and facultative anaerobic bacteria.
Further, anaerobic bacteria includes staphylococcus aureus, EHEC, fertile formula staphylococcus, VREF, copper
Green pseudomonad, aeromonas salmonicida, Coriolis grape ball Coriolis subspecies and one kind of enterobacter cloacae kind or at least two；It is aerobic
Bacterium and facultative anaerobic bacteria include Escherichia coli, baseball bacterium, streptococcus and one kind of staphylococcus kind or at least two.
The blood Bacteria Culture detection method that the present invention is provided, passes through real-time gas pressure in lasting measure flashing lightning magnetic field detector
By force, if gas pressure intensity changes, directly judge there is bacterium in blood, not only need not add it in flashing lightning magnetic field detector
Its material, reduce error source, while simplifying detecting step, reduce testing cost, it is ensured that testing result it is special
Property, the accuracy of detection efficiency and testing result can be significantly improved.
The application for the blood Bacteria Culture detection method that the present invention is provided, the blood bacterium that can be provided by the present invention is trained
Support detection method and determine the presence or absence of aerobic bacteria, anaerobic bacteria and facultative anaerobic bacteria in blood, not only need not be in flashing lightning magnetic field detector
Other materials are added, error source is reduced, while simplifying detecting step, reduces testing cost, it is ensured that testing result
Specificity, the accuracy of detection efficiency and testing result can be significantly improved.
Brief description of the drawings
Fig. 1 is the graph of a relation of Escherichia coli incubation time and gas compartment pressure in checking example 1.
Embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the present invention.It is unreceipted specific in embodiment
Condition person, the condition advised according to normal condition or manufacturer is carried out.Agents useful for same or the unreceipted production firm person of instrument, be
The conventional products that can be obtained by commercially available purchase.
According to an aspect of the present invention, the invention provides a kind of blood Bacteria Culture detection method, by determining blood
Whether the gas pressure intensity in liquid blake bottle changes, and judges to whether there is bacterium in blood, is wherein held in flashing lightning magnetic field detector
There are blood and culture medium.
During blood culture, if there is bacterium in blood, bacterial metabolism can produce carbon dioxide.Present medical treatment detection machine
Structure is reacted using substance that show color with carbon dioxide, with the change in concentration of indirect determination carbon dioxide, and then judges blood
The presence or absence of middle bacterium.Existing detection method needs to add other materials, such as fluorescence during flashing lightning magnetic field detector makes
Matter, substance that show color or radioactive marker substance, these, which are disposably detected in the processing procedure of material after use, easily causes ring
Pollute in border.Further, since these detection methods are the change in concentration using other materials indirect reaction carbon dioxide, cause inspection
Survey step is numerous and diverse, testing cost is high, the increase of error source, and poor specificity as a result influences clinical timely treatment and rescue.
The blood Bacteria Culture detection method that the present invention is provided, by determining whether the gas pressure intensity in flashing lightning magnetic field detector is sent out
Changing, directly judges to whether there is bacterium in blood, other materials need not be not only added in flashing lightning magnetic field detector, are reduced
Error is originated, while simplifying detecting step, is reduced testing cost, it is ensured that the specificity of testing result, can significantly be carried
The accuracy of high detection efficiency and testing result.
In addition, the blood method of detecting bacterium that the present invention is provided, without adding radioactive substance or fluorescence in blake bottle
Material, changes carbon dioxide receptor without frequent, greatly reduces environmental pollution.
The principle for the blood Bacteria Culture detection method that the present invention is provided is：When carrying out blood culture in blake bottle, such as blood
There is bacterium in liquid, bacterium can breed rapidly in the case of nutritional sufficiency, carry out metabolism and produce carbon dioxide, so as to lead
Cause the gas pressure intensity in flashing lightning magnetic field detector to change, if gas pressure intensity increases, illustrate that gas gross increases in flashing lightning magnetic field detector
Plus, you can judge there is bacterium in blood, if gas pressure intensity is constant in flashing lightning magnetic field detector, illustrate the gas in flashing lightning magnetic field detector
Total amount does not change, then judges bacterium is not present in blood.
It should be noted that the flashing lightning magnetic field detector being previously mentioned in the present invention is closed flashing lightning magnetic field detector.
In a preferred embodiment of the invention, blood Bacteria Culture detection method comprises the following steps：
(a) blood injection is contained with the flashing lightning magnetic field detector of culture medium and be well mixed, determined immediately in flashing lightning magnetic field detector
The initial gas pressure P of the gas compartment0；
(b) blood Bacteria Culture is carried out, and continues to determine real-time gas pressure P in flashing lightning magnetic field detectort, work as Pt﹥ P0When, then
Judge there is bacterium in blood.
In a preferred embodiment of the invention, blood is fresh in vitro blood, the bacterium that culture medium is determined needed for
Different types of culture medium may be selected in the difference of species.
In a preferred embodiment of the invention, after blood injection is contained with the flashing lightning magnetic field detector of culture medium, immediately
Determine the initial gas pressure values P of the gas compartment in flashing lightning magnetic field detector0；If there is bacterium in blood, bacterial metabolism produces dioxy
The gas changed in carbon, flashing lightning magnetic field detector increases, gas pressure intensity increase, so that the gas pressure intensity P monitored in real timet﹥ P0, you can
Judge there is bacterial growth in blood.If the gas gross being not present in blood in bacterium, flashing lightning magnetic field detector keeps constant, in real time
Gas pressure intensity also keeps constant, then continues to monitor, if until monitoring cycle (generally 7 days) terminates, real-time gas pressure PtStill
With initial gas pressure P0It is identical, then judge there is no bacterial growth in blood.
In a preferred embodiment of the invention, the top of flashing lightning magnetic field detector has retained the gas compartment, and the gas compartment accounts for blood
The 1/3-1/2 of liquid blake bottle volume, preferably 1/3.
In order to strengthen the accuracy of testing result, there should be enough irradiation spaces by the laser of the gas compartment, through many
Secondary verification experimental verification, when the gas compartment accounts for the 1/3-1/2 of flashing lightning magnetic field detector volume, had not both interfered with the growth of bacterium in blood culture
Breeding, ensure that the accuracy of testing result again, it is furthermore preferred that when the gas compartment accounts for the 1/3 of flashing lightning magnetic field detector volume, its
Most beneficial for Bacteria in Blood growth and breeding and make the accuracy of testing result optimal.
The preferred embodiment for the present invention use laser line determine flashing lightning magnetic field detector in gas pressure intensity measuring principle for：
In step (a), the initial gas pressure P of the gas compartment in flashing lightning magnetic field detector is determined0, comprise the following steps：
(s) using the gas compartment on laser illumination flashing lightning magnetic field detector top；
(m) laser intensity through the gas compartment is detected by detector；
(n) laser intensity is subjected to data conversion, obtains the initial gas pressure P of the gas compartment0。
In the preferred embodiment of the present embodiment, in step (n), laser intensity is first obtained two by data processing
The line width of carbonoxide absorption line, then the line width of carbon dioxide absorption spectral line is converted into the initial pressure P of the gas compartment0。
The principle that laser intensity obtains carbon dioxide absorption line width by data processing is：Beer-Lamber laws,
The law describes the relation transmission and incident light spectrum intensity when laser beam is through uniform dielectric：
Wherein, I0(ν) [mW] is laser light incident light intensity；I [mW] is that laser passes through the transmitted light intensity after absorbing medium；P
[atm] is gas compartment pressure；X [%] is gas concentration；S[cm-2/ atm] it is strong for the line of absorption line, can be according to HIRAN numbers
Obtained according to storehouse；L [cm] is the interaction length of laser and gas medium；φ (v) is absorption line area normalization function, because
Pressure influence is occupied an leading position, then φ (v) is Lorentz line styles：
Wherein, v0[cm-1] is the centre frequency of absorption line, and v [cm-1] is the instantaneous frequency that laser is exported, Δ v [cm-
1] be absorption line line width (FWHM), its pressure positive correlation with the gas compartment：
P=Δ v/ β
Wherein, β is gas pressure intensity ceofficient of spread.When impact severity increase between gas compartment pressure increase, gas molecule,
Then gas absorption spectrum line line width is caused further to increase.Therefore, it is possible to pass through public by the line width of carbon dioxide absorption spectral line
Formula calculates and obtains gas compartment pressure.
It should be noted that when carrying out blood Bacteria Culture, gas compartment real-time gas pressure P in flashing lightning magnetic field detectort's
Method of testing and step and initial gas pressure P0Method of testing and step it is identical, will not be repeated here.
In a preferred embodiment of the invention, it is strong by detecting laser through the gas compartment on flashing lightning magnetic field detector top
Degree obtains real-time gas pressure PtPrinciple be：
Gas pressure intensity in flashing lightning magnetic field detector changes, and causes the laser through flashing lightning magnetic field detector upper gas space strong
Degree changes, and laser intensity, which changes, carries out the line width variation that carbon dioxide absorption spectral line is obtained after data processing, dioxy
The line width variation progress data processing for changing carbon absorption spectral line obtains flashing lightning magnetic field detector gas compartment pressure change, therefore, it can lead to
Detection is crossed through the laser intensity of the flashing lightning magnetic field detector gas compartment, by data processing, the real-time gas pressure of the gas compartment is obtained
Strong Pt。
It is strong by measuring laser through flashing lightning magnetic field detector upper gas space in the preferred embodiment of the present embodiment
Degree is converted to gas compartment pressure, takes full advantage of the characteristics of spectral response speed fast and high sensitivity, can be when most short
The interior bacterium for detecting minimum, improves the accuracy of testing result.
In a preferred embodiment of the invention, in step (b), P is worked ast﹥ P0During+δ, judge there is bacterium in blood, its
Middle δ is measurement variance.
Because any instrument has error, therefore, in order to improve the accuracy of blood culture detection, in the preferred of the present invention
In embodiment, work as Pt﹥ P0During+δ, judge there is bacterium in blood, to avoid being reported by mistake.
δ is measurement variance, to carry out the test error sum of all appts used in blood culture detection process.
In a preferred embodiment of the invention, in step (b), the temperature for carrying out blood culture is 35-40 DEG C, is preferably
37℃。
Temperature can produce significant impact as one of key factor of bacterial growth, the change of temperature to the metabolism of bacterium,
It is embodied in：Temperature can not only influence the secretion and the absorption to nutritional ingredient of microbial metabolic products, and can change
Become the mobility of cell membrane, so as to influence the exchange of cellular material to transport.When bacterium is in optimum growth temp, growth metabolism
Speed is most vigorous, and metabolism duration is most short, when the cultivation temperature of bacterium is below or above critical growth temperature, is likely to result in micro-
Biological not growing is even dead.
Proved by test of many times, the temperature of blood culture at 35-40 DEG C, bacterial growth metabolism it is vigorous, metabolism duration compared with
Short, when the temperature of blood culture is 37 DEG C, the growth metabolism of bacterium is most vigorous, and metabolism duration is most short.
In a preferred embodiment of the invention, in step (b), real-time gas pressure PtTest period be 1-
20min/ times, preferably 2-5min/ times.
In order to promptly and accurately obtain blood Bacteria Detection result, in a preferred embodiment of the invention, by real-time gas
Body pressure PtTest period be set to 1-20min/ times, the degree of accuracy of measurement is higher, particularly by real-time gas pressure PtSurvey
When the examination cycle is set to 2-5min/ times, most timely, the degree of accuracy highest of test is tested.
According to another aspect of the present invention, present invention also offers the application of above-mentioned blood Bacteria Culture detection method,
The blood Bacteria Culture detection method, which is used to determine, whether there is anaerobic bacteria, aerobic and facultative anaerobic bacteria in blood.
Aerobic bacteria has more perfect respiratory enzyme system, needs molecular oxygen to do hydrogen acceptor, can only be grown under aerobic conditions numerous
Grow；Anaerobic bacteria lacks perfect respiratory enzyme system, and hydrogen acceptor is organic matter, can only growth and breeding under anaerobic；It is facultative to detest
Oxygen bacterium has perfect respiratory enzyme system, can be grown in aerobic and oxygen-free environment.
In a preferred embodiment of the invention, anaerobic bacteria includes staphylococcus aureus, EHEC, fertile formula grape
Coccus, VREF, pseudomonas aeruginosa, aeromonas salmonicida, the one of Coriolis grape ball Coriolis subspecies and enterobacter cloacae kind
Kind or at least two；Aerobic bacteria and facultative anaerobic bacteria include Escherichia coli, baseball bacterium, streptococcus and staphylococcus kind one kind or
At least two.
The application for the blood Bacteria Culture detection method that the present invention is provided, the blood bacterium that can be provided by the present invention is trained
Support detection method and determine the presence or absence of aerobic bacteria, anaerobic bacteria and facultative anaerobic bacteria in blood, not only need not be in flashing lightning magnetic field detector
Other materials are added, error source is reduced, while simplifying detecting step, reduces testing cost, it is ensured that testing result
Specificity, the accuracy of detection efficiency and testing result can be significantly improved.
With reference to specific embodiment, the present invention is described further.
Embodiment 1
A kind of blood Bacteria Culture detection method is present embodiments provided, is carried out in accordance with the following steps：
(a) blood injection is contained with the flashing lightning magnetic field detector of culture medium and be well mixed, and make the mixed of blood and culture medium
Compound accounts for the 2/3 of flashing lightning magnetic field detector, and the top of flashing lightning magnetic field detector retains the gas compartment for having 1/3, laser is opened immediately, makes to swash
The gas compartment in light irradiation flashing lightning magnetic field detector, and the laser intensity through the gas compartment is detected by detector, detector will be detected
To laser intensity be sent to computer carry out data processing, be converted into initial gas pressure P0；
(b) flashing lightning magnetic field detector is put into 37 DEG C of constant incubator and carries out blood culture, and a blood is determined every 3min
The real-time gas pressure P of the gas compartment in liquid blake bottlet, real-time gas pressure PtThe same P of method of testing0, will not be repeated here；
Work as Pt﹥ P0During+δ, judge there is bacterium in blood, wherein δ is measurement variance, the measurement variance refer to laser, detector and
The measurement variance sum of computer, works as P0＜ Pt＜ P0During+δ, judge bacterium is not present in blood.
In the present embodiment, the flashing lightning magnetic field detector used is 50ml blake bottle；The culture medium used is by poly- anethole
Sodium sulfonate, menadione, soybean casein digestive juice, brain heart infusion, the wing propylhomoserins of L- half, hydrochloric acid pyrrole chop alcohol, hemin and other are molten
Constituted in the compound amino acid and carbohydrate of pure water.
The blood Bacteria Culture detection method that the present embodiment is provided, Bacteria Culture is carried out using closed flashing lightning magnetic field detector,
Other materials need not be added in flashing lightning magnetic field detector in detection process, detecting step is simplified, error source, drop is reduced
Low testing cost；In addition, the blood Bacteria Culture detection method that the present embodiment is provided, flashing lightning magnetic field detector is detected by detector
The laser intensity of upper gas space, and gas compartment pressure is obtained by data processing, take full advantage of spectral response speed
The characteristics of fast and sensitivity is high, the bacterium of minimum can be detected within the most short time, the specificity of testing result is improved
And accuracy.
Verify example 1
This checking example exemplified by being inoculated with Escherichia coli in blood, the blood Bacteria Culture detection side that the checking present invention is provided
The accuracy of method, the initial gas pressure P that this checking example is used0With real-time gas pressure PtMethod of testing with embodiment 1
It is identical, it will not be repeated here.
The blood Bacteria Culture detection method that this checking example is provided, is carried out according to the following steps：
(a) Escherichia coli are inoculated into 50ml flashing lightning magnetic field detectors, the top of flashing lightning magnetic field detector, which is retained, 1/3 gas space
Between, and make the concentration of Escherichia coli in flashing lightning magnetic field detector be 3 × 104Cfu/mL, determines the initial gas pressure of the gas compartment immediately
By force；
(b) flashing lightning magnetic field detector is put into 37 DEG C of constant incubator and cultivated, and in real time in monitoring flashing lightning magnetic field detector
Gas compartment pressure, and according to the Bacteria Culture time record respectively 2h, 4h, 6h, 8h, 10h, 12h, 14h, 16h, 18h, 20h,
22h, 24h, 26h, 28h and 30h gas compartment pressure, the gas pressure intensity value of measured different periods are as shown in table 1：
The different incubation time gas compartment pressure data tables of table 1
According to Bacteria Culture time in table 1 and the relation of gas compartment pressure, set up using incubation time as abscissa, with gas
The pressure in body space is mapped for the rectangular coordinate system of ordinate, is obtained Fig. 1, be can be seen that with reference to table 1 and Fig. 1 as blood is thin
Gas pressure intensity increase in the growth of bacterium incubation time, flashing lightning magnetic field detector, this and growth of the Bacteria in Blood with incubation time,
Gas pressure intensity increase in the carbon dioxide increasing number discharged, flashing lightning magnetic field detector matches, the present invention checking of this explanation
The blood Bacteria Culture detection method that example is provided can quickly determine whether pressure in blood changes, so as to carry out Bacteria in Blood
The judgement whetheing there is, need not only add other materials in flashing lightning magnetic field detector, error source be reduced, while simplifying detection
Step, reduces testing cost, it is ensured that the specificity of testing result, can significantly improve the standard of detection efficiency and testing result
True property.
It should be noted that the training that the equal be the same as Example 1 of culture medium and flashing lightning magnetic field detector that this checking example is used is used
Base and flashing lightning magnetic field detector are supported, be will not be repeated here.
Finally it should be noted that：Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations；To the greatest extent
The present invention is described in detail with reference to foregoing embodiments for pipe, it will be understood by those within the art that：Its according to
The technical scheme described in foregoing embodiments can so be modified, or which part or all technical characteristic are entered
Row equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology
The scope of scheme.
Claims (10)
1. a kind of blood Bacteria Culture detection method, it is characterised in that by whether determining the gas pressure intensity in flashing lightning magnetic field detector
Change, judge to whether there is bacterium in blood, be wherein contained with blood and culture medium in flashing lightning magnetic field detector.
2. blood Bacteria Culture detection method according to claim 1, it is characterised in that comprise the following steps：
(a) blood injection is contained with the flashing lightning magnetic field detector of culture medium and be well mixed, gas in flashing lightning magnetic field detector is determined immediately
The initial gas pressure P in space0；
(b) blood Bacteria Culture is carried out, and continues to determine real-time gas pressure P in flashing lightning magnetic field detectort, work as Pt﹥ P0When, then judge
There is bacterium in blood.
3. blood Bacteria Culture detection method according to claim 2, it is characterised in that the top of the flashing lightning magnetic field detector
The gas compartment is retained, the gas compartment accounts for the 1/3-1/2 of flashing lightning magnetic field detector volume, preferably 1/3.
4. blood Bacteria Culture detection method according to claim 3, it is characterised in that in step (a), determines blood
The initial gas pressure P of the gas compartment in blake bottle0, comprise the following steps：
(s) using the gas compartment on laser illumination flashing lightning magnetic field detector top；
(m) laser intensity through the gas compartment is detected by detector；
(n) laser intensity is subjected to data conversion, obtains the initial gas pressure P of the gas compartment0。
5. blood Bacteria Culture detection method according to claim 4, it is characterised in that in step (n), first by laser
Intensity by data conversion into carbon dioxide absorption spectral line line width, then by the line width of carbon dioxide absorption spectral line pass through data turn
It is changed to the initial pressure P of the gas compartment0。
6. blood Bacteria Culture detection method according to claim 2, it is characterised in that in step (b), work as Pt﹥ P0+δ
When, judge there is bacterium in blood, wherein δ is measurement variance.
7. blood Bacteria Culture detection method according to claim 2, it is characterised in that in step (b), enters promoting circulation of blood training
Foster temperature is 35-40 DEG C, preferably 37 DEG C.
8. blood Bacteria Culture detection method according to claim 2, it is characterised in that in step (b), real-time gas
Pressure PtTest period be 1-20min/ times, preferably 2-5min/ times.
9. the application of the blood Bacteria Culture detection method according to any one of claim 1-8, it is characterised in that be used for
Determine and whether there is anaerobic bacteria, aerobic bacteria and facultative anaerobic bacteria in blood.
10. the application of blood Bacteria Culture detection method according to claim 9, it is characterised in that anaerobic bacteria includes gold
Staphylococcus aureus, EHEC, fertile formula staphylococcus, VREF, pseudomonas aeruginosa, aeromonas salmonicida, Coriolis
Grape ball Coriolis subspecies and one kind of enterobacter cloacae kind or at least two；Aerobic bacteria and facultative anaerobic bacteria include Escherichia coli,
Baseball bacterium, one kind of streptococcus and staphylococcus kind or at least two.
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| 韩叶星: "基于可调谐半导体激光吸收光谱的血培养检测", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
| 项金冬: "基于光谱技术的微生物生长检测研究", 《中国优秀硕士学位论文全文数据库 基础科学辑》 * |
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