CN105876253A - Feed additive capable of improving egg laying performance of laying hens and preparation method of feed additive - Google Patents
Feed additive capable of improving egg laying performance of laying hens and preparation method of feed additive Download PDFInfo
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Abstract
The invention relates to a feed additive capable of improving the egg laying performance of laying hens and a preparation method of the feed additive and belongs to the field of livestock and poultry breeding. In order to overcome the problem of drug resistance of antibiotics, which widely exists in an existing laying hen breeding industry, and change a condition that a product for improving the laying rate and the laying quality of the laying hens, particularly Yellow River estuary laying hens, is lacked in the market for a long period, the invention provides the feed additive capable of remarkably improving the egg laying performance of the laying hens; and the feed additive is prepared from the following traditional Chinese medicine components: semen perillae, polygonum cuspidatum, polygonum multiflorum, Chinese yams, astragalus mongholicus, rheum officinale, prepared liquorice roots, Chinese dates and black sesames. When the feed additive provided by the invention is used for feeding the Yellow River estuary laying hens, the laying rate and the laying quality can be improved; and the feed intake and the feed-egg ratio of Yellow River estuary black-bone chickens can be remarkably improved, and the feed additive has no side effect or drug resistance.
Description
Technical field
The present invention relates to a kind of feed addictive improving Layer Production Performance and preparation method thereof, be specifically related to a kind of raising Huang
Chinese herbal feed additive of river mouth black-bone chicken egg laying performance and preparation method thereof, belongs to livestock and poultry cultivation field.
Background technology
Along with the fast development of modern feed industry, the feed addictive of a large amount of Prof. Du Yucang such as antibiotic, somatotropin, expelling parasite
Agent, hormone, flavor enhancement, modifying agent, preservative generally add in feed.The application of these additives, to animal husbandry
Development serves huge impetus undoubtedly.But, along with research deepen continuously and produce in produced problem, people
The most gradually recognize the negative effect that these additives bring.Particularly a large amount of uses of antibiotic, even abuse, lead
Cause the drug resistance and the residual that produce and caused vast researcher to the harm that brings of humans and animals health and disappeared
The concern of the person of expense and worry.
Compared with broiler chicken, the production cycle of laying hen is long, and the various undesirable elements suffered are more, and on a large scale, highdensity raise
Support and also bring numerous potential safety hazard, cause various paathogenic factor long-standing problem breeding layer chicken.In order to pursue greatest benefit,
Production is generally added in layer diets the feed addictive of various chemical synthesis, to reaching prevention and treatment disease, carrying
The production performance of high egg fowl, improve the purposes such as Egg Quality and the requirement of sense meeting consumer.Laying rate of laying hen and Egg Quality are not
Only affected by abuse of antibiotics, also affected by oneself factor and external environment, then find a green approach and come
Improve laying rate of laying hen and quality of laying eggs is extremely the most urgent.
The Chinese herbal medicine resource of China is the abundantest, and Chinese herbal feed additive is many adopts directly or indirectly in plant.It is protected
Naturality and the biologically active of various composition are held, it is easy to body absorbs.Even if the material that cannot be absorbed by organisms, also
Can excrete smoothly.The impact of laying rate of laying hen and quality of laying eggs has been carried out deeply grinding by many researchers by Chinese herbal medicine
Study carefully.As added Chinese herbal medicine extract " green Yikang " in layer diets, (medicine consists of Radix Isatidis rheum officinale, Herba Andrographitis, smalt
Leaf etc.), addition is 1000-2000g/t, can improve laying rate of laying hen, dramatically increase average egg weight, hence it is evident that improve feedstuff-egg ratio.
The nutriment contained in Chinese herbal medicine can make up the deficiency of some nutritional labelings in feed, and some of which enzyme and perfume (or spice)
Taste material can increase feed intake, promotes digestion and the absorption of nutriment；Wang Fengxia etc. (2009) are in chick basal diet
Adding the Folium Astragali powder of 1%, 3%, 5%, result shows when 21 age in days, and the feedstuff-meat ratio of Radix Astragali group is all substantially less than comparison
Group P < 0.05).This result of the test also indicates that, adding compound feed addictive of the present invention can a certain degree of material reducing laying hen
Egg ratio.Wang De has just waited (2006) to be made into 1% with Radix Codonopsis, the Radix Astragali etc. and trace element, multivitamin, amino acid etc.
Premix is added in Roman egghen complete diet pellet, and result of the test shows: add Chinese herbal medicine group can significantly reduce egg soft egg,
Broken egg, deformity egg rate, and extend the Egg Production of Laying Hens cycle.But the medium-height grass in market regrettably, can be passed at present
Medicine feed addictive is the most few, and the impact of its egg quality and laying rate produces change along with the difference of layer breed,
The most even have little effect.
" HUANGHE ESTUARY black-bone chicken " be started from 2000 to cultivate based on the laying hen black-bone chicken laid eggs, be black with black-food
On the basis of chicken, cultivate formed with common egg, there is the new silkies strain of different eggshell.Selecting local stupid chicken is female parent,
It is male parent with multiple excellent Local Chicken Breeds, with the help of multidigit animal husbandman, through bumpy adhering in 9 years, meticulously selects
Educate and repeatedly hybridize, finally cultivated the excellent black-bone chicken of satisfaction, laying hen strain new species in 2012.Its feature is: crow
Chicken, its kindred, skin all crow look, build U-shaped, full-fledged variegated, black leg, 5 pawls, mature rooster 2.25 kilograms
Left and right, hen about 1.75 kilograms, belong to medium-sized laying hen.Chick goes out shell and weighs 32 grams, and 60 ages in days weigh 612 grams, opens the product date
About 140 days, laying eggs about 200 egg laying performance year, average 45 grams of egg size, eggshell is that green (with duck's egg look) eggshell is former
For 0.32mm, it it is a most exclusive excellent indigenous chicken strain.Lack in the market and can improve HUANGHE ESTUARY black-bone chicken product
Egg Quality and the feed addictive of laying rate.
Summary of the invention
Feed addictive of the present invention has both the double action of trophism and Drug.Trophism is mainly reflected in it and contains animal
Grow necessary various nutriments, such as protein, amino acid, vitamin and trace element etc..Drug effect
The various bioactivators (alkaloid, organic acid, polysaccharide etc.) contained with it are relevant.Feed addictive the most of the present invention
Also have and improve feed local flavor, palatability, anti-feed oxide and the function such as go mouldy.Chinese herbal feed additive is how effective with it
Composition, from the metabolism of many links interference pathogenic microorganism such as ribonucleic acid, DNA, energy conversion, thus reaches
Purpose to restraining and sterilizing bacteria.Its composition is the most harmonious with animal body, it is easy to is absorbed by organisms and utilizes, non-absorbent also
Can excrete smoothly.To so far, it has been recognized that it is without the resistance to the action of a drug.
In order to overcome the problem of the antibiotic resistance being widely present in existing breeding layer chicken industry, change and lack on market for a long time
Few product to laying hen particularly HUANGHE ESTUARY laying rate of laying hen with the raising of quality of laying eggs, the present invention provides one significantly to carry
The feed addictive of high Layer Production Performance, this feed addictive can improve laying rate and chicken when HUANGHE ESTUARY black-bone chicken is raised
Egg size amount, and feed intake and the feedstuff-egg ratio of HUANGHE ESTUARY black-bone chicken can be significantly improved, and produce without any side effect or drug resistance
Raw.Similar technique effect can be produced when this feed addictive is used for other layer breedings by applicant.
The present invention solves above-mentioned technical problem by the following technical programs:
A kind of feed addictive improving Layer Production Performance, it prepares by following traditional Chinese medicinal components: sub-Soviet Union, giant knotweed, what
The tuber of multiflower knotweed, Chinese yam, the Radix Astragali, rheum officinale, honey-fried licorice root, date, Semen sesami nigrum.
Feed addictive of the present invention is according to Chinese herbal medicine incompatibility principle, according to the four natures and five flavors of drug of medicine, the monarch of prescription,
Minister, help, make to combine formulated, the nutritional quality of egg can be increased substantially when laying hen feeds.In order to enter one
Step improves the quality-improving effect of feed addictive, and applicant has carried out formula and explored above-mentioned feed addictive, it is preferable that
Described feed addictive is prepared by following traditional Chinese medicinal components: sub-Soviet Union 8-12 part, giant knotweed 15-19 part, fleece-flower root 13-17
Part, Chinese yam 10-20 part, Radix Astragali 5-9 part, rheum officinale 11-17 part, honey-fried licorice root 12-16 part, date 10-20 part, Semen sesami nigrum
10-20 part.
As the preferred a kind of embodiment of the present invention, described feed addictive is prepared by following traditional Chinese medicinal components: son
Revive 10 parts, giant knotweed 17 parts, the fleece-flower root 15 parts, Chinese yam 15 parts, the Radix Astragali 7 parts, rheum officinale 14 parts, honey-fried licorice root 14 parts,
15 parts of date, Semen sesami nigrum 15 parts.
The present invention also provides for the preparation method of a kind of above-mentioned feed addictive, and it comprises the steps: simple through 105 DEG C
Dry, pulverize, cross 80 mesh sieves, mix according to traditional Chinese medicinal components proportioning, to obtain final product.
The present invention also provides for the application of a kind of above-mentioned feed addictive, and i.e. described feed addictive feeds for laying hen.Wherein
Described laying hen is preferably HUANGHE ESTUARY black-bone chicken.Wherein when laying hen feeds, the weight ratio of feed addictive and basal diet is
0.005-0.015:1.The embodiment of the present invention 6 have studied feed addictive to HUANGHE ESTUARY black-bone chicken by HUANGHE ESTUARY black-bone chicken administering transgenic
Quality of laying eggs and the impact of laying rate, it was found that the feed addictive of the present invention adding variable concentrations gradient all can improve
The laying rate of laying hen, egg production, and relatively control group is compared, and improves the thick protein of each test group, calcium, the apparent digestion of phosphorus
Rate.Efficiency of feed utilization can be significantly improved it addition, the present invention is combined feed addictive of the present invention, improves performance in layers.
The present invention compared with prior art it is a technical advantage that:
(1) on the basis of the fine quality laying hen that place is cultivated, it is thus achieved that there is the feed compound of the present invention of good result
Additive, and determine the suitableeest addition.The feed addictive of the present invention adding variable concentrations gradient all can improve the product of laying hen
Egg rate, egg production.Compared with control group, test group experimental period laying rate is significantly improved, and feedstuff-egg ratio conspicuousness reduces.
For the digestibility of nutriment, relatively control group is compared, and improves the thick protein of each test group, calcium, phosphorus is apparent disappears
Rate, between each group, the apparent digestibility difference of crude fat is all not up to the level of signifiance.
(2) present invention is combined feed addictive of the present invention and can significantly improve efficiency of feed utilization, improve performance in layers.
After adding feed addictive of the present invention in daily ration, eggshell is relatively heavy, Hough unit, yolk coefficient, yolk are relatively heavy, egg white
In (yellow) water content, egg white (yellow), in protein content, yolk, fat/fresh egg size is on the rise compared with control group；Yolk
Color all has clear improvement.Cholesterol Content in Egg Yolk significantly reduces, and along with the prolongation effect of the time of interpolation becomes apparent from.
(3) achievement in research of the present invention is to develop novel fodder additive application on HUANGHE ESTUARY black-bone chicken to provide newly
Theoretical foundation.HUANGHE ESTUARY black-bone chicken is high-quality indigenous cultivation kind, along with the fast development of breeding layer chicken industry, by food pollution institute
The livestock and poultry and the product security incident that cause also are risen one after another, and develop livestock and poultry nuisance free feed supplement product, for
Animal products upgrading is significant.
Detailed description of the invention
Further illustrate the present invention below by way of specific embodiment, but described embodiment never in any form the restriction present invention are special
The scope of profit protection.
Embodiment 1 feed addictive of the present invention and preparation method thereof
Feed addictive is prepared by following traditional Chinese medicinal components: sub-Soviet Union 8 parts, giant knotweed 15 parts, the fleece-flower root 13 parts, Chinese yam 10
Part, the Radix Astragali 5 parts, rheum officinale 11 parts, honey-fried licorice root 12 parts, 10 parts of date, Semen sesami nigrum 10 parts.
The preparation method of described feed addictive comprises the steps: simple is dried, pulverized through 105 DEG C, cross 80 mesh
Sieve, mixes according to traditional Chinese medicinal components proportioning, to obtain final product.
Embodiment 2 feed addictive of the present invention and preparation method thereof
Feed addictive is prepared by following traditional Chinese medicinal components: sub-Soviet Union 12 parts, giant knotweed 19 parts, the fleece-flower root 17 parts, Chinese yam
20 parts, the Radix Astragali 9 parts, rheum officinale 17 parts, honey-fried licorice root 16 parts, 20 parts of date, Semen sesami nigrum 20 parts.
Preparation method is with embodiment 1.
Embodiment 3 feed addictive of the present invention and preparation method thereof
Feed addictive is prepared by following traditional Chinese medicinal components: sub-Soviet Union 10 parts, giant knotweed 17 parts, the fleece-flower root 15 parts, Chinese yam
15 parts, the Radix Astragali 7 parts, rheum officinale 14 parts, honey-fried licorice root 14 parts, 15 parts of date, Semen sesami nigrum 15 parts.
Preparation method is with embodiment 1.
Embodiment 4 feed addictive of the present invention and preparation method thereof
Feed addictive is prepared by following traditional Chinese medicinal components: sub-Soviet Union 8 parts, giant knotweed 19 parts, the fleece-flower root 13 parts, Chinese yam 20
Part, the Radix Astragali 5 parts, rheum officinale 17 parts, honey-fried licorice root 12 parts, 20 parts of date, Semen sesami nigrum 10 parts.
Preparation method is with embodiment 1.
Embodiment 5 feed addictive of the present invention and preparation method thereof
Feed addictive is prepared by following traditional Chinese medicinal components: sub-Soviet Union 12 parts, giant knotweed 15 parts, the fleece-flower root 17 parts, Chinese yam
10 parts, the Radix Astragali 9 parts, rheum officinale 11 parts, honey-fried licorice root 16 parts, 10 parts of date, Semen sesami nigrum 20 parts.
Preparation method is with embodiment 1.
HUANGHE ESTUARY black-bone chicken is laid eggs the impact of quality and laying rate by embodiment 6 feed addictive of the present invention
Experimental animal: test is carried out in Dongying Lan Bao agricultural science and technology Co., Ltd's breeding layer chicken field, selects 400 healthy Huangs
River mouth black-bone chicken, three layers of full staged are raised in cages.
1 experimental design forms with daily ration
1. experimental design: test and have design iterations for single-factor, selects the HUANGHE ESTUARY black-bone chicken of 400 50 week old, sees through one week
Examine egg production the most close, healthy anosis, body weight size is basically identical, is randomly divided into 4 groups, often group 100, in group
If 5 repetitions, each repetition 20, organizing I i.e. control group fed basal diet, group II feeds basal diet+0.5% and sends out
Bright feed addictive, group III feeds basal diet+1.0% feed addictive of the present invention, and group IV feeds basal diet+1.5%
Invention feed addictive, wherein said feed addictive of the present invention prepares according to the preparation method described in embodiment 3.
10 days preliminary trial periods, test from the beginning of 20 days July in 2015, August 29 in 2015 end of day, 40 days by a definite date.
Table 1 experimental design
2. daily ration composition: basal diet is corn-soybean meal diet, daily ration composition and trophic level are shown in Table 2.
Table 2 experimental basis daily ration composition and trophic level
Note: containing Fe 45mg in per kilogram basal diet；Zn 28mg；Mn 35mg；Cu 5mg；Se 0.08mg；
I 0.28mg；Vitamin A 6000IU；Vitamin D32600IU；Vitamin E 26mg；Vitamin K327mg；Dimension
Raw element B10.7mg；Cobastab23.6mg；Cobastab127.5mg；Folic acid 13mg；Pantothenic acid 6mg.
2 feedings and managements
Using the method that artificial lighting and natural lighting combine, illumination every day 16h, force ventilation adds gravity-flow ventilation, freely
Search for food, in vain by drinking water.Monitoring chicken group's health status, record is dead washes in a pan chicken number and reason thereof, periodically carries out routine disinfection in house.
Every day 6:00,11:00,17:00 timing feeding, before feeding weigh residual feed, add up every daily material consumption.9:00,14:00
Temperature in record hen house, 10:00,17:30 timed collection egg, record often repeat egg number, emblem of laying eggs, broken soft egg number and
Chicken number, calculates the laying rate of every day, breakage rate simultaneously.
3 Testing index and method
Record egg size, number of laying eggs, inventory, residue doses, livestock on hand chicken number, calculating average egg weight, laying rate (include
Damaged egg) average daily ingestion amount, feedstuff-egg ratio (including damaged egg).
Egg size: utilize electronic balance weighing egg size, is accurate to 0.01 gram.
Laying rate (%)=(every day egg number/livestock on hand chicken number) × 100
The feed consumption rate of feedstuff-egg ratio=weekly/weight of laying eggs weekly
4 daily ration Nutrient Apparent Digestibilities
10～12d, 20～22d in the feeding experiment phase collects test chicken excreta, sample refrigeration every day continuously respectively
It is stored in-4 DEG C of refrigerators, (notes to contain the impurity such as grittiness during the collection of excrement sample, and pick after within three days, collecting
Feather etc.), sample mix is uniform.Sample drying makes air-dry sample, pulverizes 40 mesh sieves, loads sealed bag
Standby survey.Use endogenous indicator method (4N-HCl insoluble ash) measure thick protein in daily ration and excreta, calcium, phosphorus,
The apparent digestibility of crude fat.Assay method sees " forage analysis and determination of feeds quality technology ".
Endogenous indicator method metabolic rate measures (4N-HCL insoluble ash method), and computing formula is as follows:
Certain nutrient metabolizability (%)=100-100 (b × c)/(a × d)
A: certain nutrient content in feed；C: acid insoluble ash content in feed
B: certain nutrient content in excrement sample；D: acid insoluble ash content in excrement sample
5 data process&analysis
ANOVA model in SPSS16.0 software is used to carry out the one-factor analysis of variance (percentage first carries out arcsine transformation),
Using LSD Multiple range test, data represent with average ± standard deviation.
6 results and analysis
The feed addictive the most of the present invention impact on HUANGHE ESTUARY black-bone chicken laying rate
This test selects 50 week old to lay eggs mid and late aged layer, before on-test each group laying rate oneself downward trend occurs.Such as table 1
Shown in, l week group III (72.56%) laying rate is minimum, and group IV (75.32%) is the highest, and each group difference is not notable (P > 0.05)；
III laying rate of laying hen is significantly raised reaches 79.42% for 2nd week group, improves 5.30 percentage points than control group 74.12%, difference
Significantly (P<0.05), group II, group IV be not more notable (P>0.05) than control group improves 1.89,4.69 percentage points of differences respectively,
Compared with l week, test group laying rate all has improvement, and control group still has downward trend；Within 3rd week, ii I, IV group are laid eggs
Rate respectively higher by 5.13% than control group, 8.06%, significant difference (P<0.05), group II and each group of difference are notable (P>0.05)；
Within 4th week, start feed addictive group laying rate and be all remarkably higher than control group (P < 0.05)；5th week, temperature significantly raised, right
According to group laying rate dramatic decrease, although each feed addictive group laying rate has fallen after rise, but still pole is significantly higher than control group
(P<0.01)；Within 6th week, feed addictive group laying rate has asked liter, and control group continues to drop to 66.36%, difference pole
Significantly (P < 0.01)；From the point of view of the full phase, ii, III, IV group laying rate are respectively 75.51%, 76.75%, 77.63%,
Exceeding 4.14,5.38,6.26 percentage points than control group 71.37%, difference is extremely notable (P < 0.01), it is seen that feed of the present invention
Additive can significantly improve the laying rate decline that HUANGHE ESTUARY black-bone chicken causes because of week old increase and summer high temperature.
The impact on laying rate of laying hen of table 3 compound Chinese herbal medicinal feed additive
Note: same column indicates same letter and represents that difference is not notable (P > 0.05), indicates different lowercase alphabet and shows significant difference
(P < 0.05), indicates different capitalization and represents that difference is extremely notable (P < 0.01).Lower same.
Feed addictive the most of the present invention is on HUANGHE ESTUARY black-bone chicken egg size, egg production, feed intake, the impact of feedstuff-egg ratio
Feed addictive of the present invention has no significant effect (P < 0.05) to the average egg weight of each test group, deformity egg number, to group I,
The laying rate impact of II is extremely notable (P < 0.01), and the laying rate impact on group III is notable (P < 0.05), lays eggs group IV
Rate impact is notable (P>0.05), and organizes the laying rate significant difference (P<0.05) of IV and group I, II；In terms of broken egg number,
Group II and group III significant difference (P < 0.05)；In terms of soft egg number, group I, II, III and control group difference are extremely notable (P < 0.01),
Group III and to process difference between I, IV extremely notable (P < 0.01)；In terms of husky lime-preserved egg, between group II and group IV, difference is the most notable
(P<0.01), it is not notable (P>0.05) that other respectively test group difference；Wash in a pan on number dead, group I and group II significant difference
(P<0.05), it is not notable (P>0.05) that other respectively test group difference；In average daily feed intake, II, III, IV are with right for group
According to group significant difference (P<0.05), group II and other each test group differences are not notable (P>0.05).Therefore, add in compound
Herbal medicine feed addictive is improved laying rate of laying hen, reduces the effect of the average daily feed intake of laying hen.
Table 4 Chinese herbal medicine is on egg size, egg production, feed intake, the impact of feedstuff-egg ratio
The feedstuff-egg ratio of test II, III, IV group have dropped 0.32%, 0.32%, 0.46% respectively, and difference is extremely notable (P < 0.01),
Ii, between III, IV, difference is notable (P > 0.05).As can be seen here, feed adds a certain proportion of feed of the present invention
Additive, is conducive to improving efficiency of feed utilization, reduces feedstuff-egg ratio.
The feed addictive the most of the present invention impact on HUANGHE ESTUARY black-bone chicken nutrition material apparent digestibility
Feed addictive of the present invention, on HUANGHE ESTUARY black-bone chicken dietary protein, calcium, phosphorus, the impact of crude fat apparent digestibility, is shown in
Table 5.
The impact (%) on Nutrient Apparent Digestibility of table 5 feed addictive of the present invention
From table 5, apparent digestibility of the crude protein, each test group relatively control group has been respectively increased 3.28%, 2.23%,
8.81% (P < 0.05), wherein group IV reaches the significance level of difference (P < 0.05).Between each test group, difference is all not up to notable
Level (P ＞ 0.05).
The apparent digestibility of calcium, each test group relatively control group has been respectively increased 3.17%, 11.47% (P < 0.05) and 13.81%
(P<0.05).Between each test group, III and IV group relatively II group has been respectively increased 8.05% (P < 0.05), 10.31% (P < 0.05).
The apparent digestibility of phosphorus, each test group relatively control group has been respectively increased 15.22% (P ＞ 0.05), 47.32% (P ＞ 0.05)
With 85.78% (P < 0.05)；Between each test group, difference is not the most notable (P ＞ 0.05).
The apparent digestibility of crude fat, between each group, difference is all not up to the level of signifiance (P ＞ 0.05).
The impact on HUANGHE ESTUARY black-bone chicken egg quality of embodiment 7 feed addictive of the present invention
(1) test material
Corresponding contents is tested with embodiment 6.
(2) experimental design forms with daily ration
Corresponding contents is tested with embodiment 6.
(3) feeding and management
Corresponding contents is tested with embodiment 6.
(4) collection of sample and process
The 10th, 20,30,40 days of test gather egg sample respectively, each repeat to randomly draw 5 pieces, label, with take with
Inspection.
(5) Testing index and method
Egg shape index: with vertical footpath and the maximum transverse diameter of vernier caliper measurement egg, be accurate to 0.02 millimeter.Egg shape index=transverse diameter/
Vertical footpath × 100%
Yolk coefficient: with the height of vernier height gauge amount yolk, the diameter of vernier caliper measurement yolk, be accurate to 0.02 milli
Rice.Yolk coefficient=height/diameter × l00%
Yolk color: Roche colorimetric fans colorimetric under fluorescent light.
Yolk is relatively heavy: egg white and yolk are kept completely separate with Yolk separator, collects yolk and weighs weight.Yolk is relatively heavy
=yolk weight/shell egg weight × 100%
Eggshell is relatively heavy: eggshell is relatively heavy=and eggshell weight/egg size × 100%
Shell thickness: measure the blunt end of egg, the shell thickness at middle and sharp two positions of end with micrometer caliper respectively.Ask it
Mean value, in units of millimeter, is accurate to 0.01mm.
Hough unit: broken by egg on the flat glass plate of horizontal positioned, measures dense albumen with albumen height analyzer high
Degree.
Hu=100 × log (H-107 × W × 0.37+7.6)
Hu: Hough unit
H: dense albumen height (mm)
W: egg size (g)
Water content: separated with yolk by egg and weigh respectively, puts in thermostatic drying chamber after 50-53 DEG C of drying 8-12h, title
Weight.
Fat/fresh egg size in yolk: boiled by egg, open after cooling, is completely exfoliated yolk and egg white, precise yolk
Weight, blends yolk and mixes thoroughly, weigh sample and put in filtration paper cylinder, is dried 3.5 hours, puts into title in 105 DEG C of thermostatic drying chambers
In the fatty bottle of good weight, use Soxhlet leaching device to extract 6 hours, take out filter paper letter, fat bottle is reentered into constant temperature and does
105 DEG C of dry case is dried 3.5 hours, takes out cooling and weighs.
Fat/fresh egg size=(W in yolk2-W1)/W3×W4/ W × 100%
W2: cool down, after extracting, the fatty bottle weight weighed；
Wl: the fatty bottle weight that before extracting, cooling is weighed；
W3: the sample weight taken after pulverizing yolk；
W4: yolk gross weight；
W: fresh egg size
The mensuration of protein content in egg white, yolk: egg is opened and is kept completely separate egg white solution and egg yolk liquid, use micropipette
Device is drawn in the digest tube that the liquid after separating is the most weighed, again claims the weight child of digest tube, then adds 6 in digest tube
The milliliter concentrated sulfuric acid and appropriate catalyst, seal, and puts into and digests 6h taking-up cooling in far infrared digesting, uses kelvin
Nitriding measures protein content.
The mensuration of Cholesterol Content in Egg Yolk: boiled by egg, separates yolk and egg white, weighs immediately after cooling.Each egg
Huang is put in beaker and is smashed to pieces, stirs.Accurately weigh rod even sample 1.0g (be accurate to after decimal point four) as to be measured
Sample.It is fully ground with methanol-chloroform (2:l) mixed solution and extracts to without obvious particle, filter to 10ml tool to fill in and carve
Degree test tube in, and supplement be diluted at 10ml scale, mixing, seal, put in 4 DEG C of refrigerators stand refrigeration 8-12h.Take
Supernatant cholesterol to be measured in test tube.With reference to cholesterol in serum assay method, use marine products ANALYTECH648 on instrument
The serum total cholesterol determination reagent that Bioengineering Research Institute produces is built up in semi-automatic biochemical analyzer, thermostat water bath, Nanjing
Box.Computational methods are cholesterol (mmmol/l) in sample=(sample cell absorbance/standard pipe absorbance) × normal concentration
(6) data process&analysis
ANOVA model in SPSS16.0 software is used to carry out the one-factor analysis of variance (percentage first carries out arcsine transformation),
Using LSD Multiple range test, data represent with average ± standard deviation.
(7) result and analysis
Feed addictive the most of the present invention is relatively heavy on egg shape index, shell thickness, eggshell, the impact of Hough unit
As shown in Table 6, after layer diets adds the feed addictive of the present invention of variable concentrations gradient, egg shape index all has difference
The raising of degree, especially the highest with group III index (78.63%), it is significantly higher than group II, control group (P < 0.05)；Group IV
And difference is not notable (P > 0.05) between each group.Group III shell thickness (0.44mm) is significantly better than group IV, control group (P < 0.05),
Though group II shell thickness increased, but respectively organize difference with other notable (P > 0.05).Layer diets adds variable concentrations
Feed addictive of the present invention after, test group eggshell relatively heavy respectively ratio control group improve 4.60%, 5.69%, 18.32%,
But difference is not notable (P > 0.05), between each test group, difference is not the most notable (P > 0.05), it can be seen that, feed of the present invention
Additive impact relatively heavy on eggshell is little；Test group Hough unit all has raising in various degree for control group,
But difference is not notable (P > 0.05).
Hough unit value is the inspection that United States Department of Agriculture's egg product standard specifies and the index evaluating egg freshness, and Hough unit is the highest,
The stickiness of albumen is the best, protein quality the highest (Huang Ren record etc., 2007).Generally Hough unit is between 75-82, high
Up to about 90, table egg is more than 72.For this test, add feed addictive of the present invention and can improve
The Hough unit of egg.
Table 6 feed addictive of the present invention is relatively heavy on egg shape index, shell thickness, eggshell, the impact of Hough unit
Feed addictive the most of the present invention to yolk color, yolk coefficient, yolk is relatively heavy, yolk water content, egg white are aqueous
The impact of amount
As shown in table 7, III group and IV group yolk color are substantially better than group II, control group, and difference is extremely notable (P < 0.01),
Group II increases relative to control group, yolk color, but difference is not notable (P > 0.05)；Test group yolk coefficient is more right
Slightly rise according to group, but difference inconspicuous (P > 0.05), show that feed addictive of the present invention is little to laying hen yolk index impacts；
Test group yolk is relatively heavy, yolk water content is on the rise, but difference is not notable (P > 0.05)；Egg white water content with compare
Group difference is not notable (P > 0.05).
The impact on yolk quality of table 7 feed addictive of the present invention
Feed addictive the most of the present invention is to content of phospholipid in fat/fresh egg size, yolk in protein, yolk in egg white (yellow)
Impact
As shown in table 8, daily ration adds the feed addictive of the present invention of variable concentrations to protein content, yolk egg in egg white
In white matter content, yolk, fat/fresh egg size affects difference significantly (P > 0.05), but can be effectively improved content of phospholipid in yolk,
Best with group IV effect, relatively organize II, control group the highest by 0.72%, 0.86%, difference extremely shows potato (P < 0.01), relatively organizes III
High by 0.36%, difference is not notable (P < 0.05)；Ii, III group and control group difference is not notable (P > 0.05).
Table 8 feed addictive of the present invention is to the comparison of protein, fat and content of phospholipid in egg white/yolk
Feed addictive the most of the present invention is on the impact of cholesterol in yolk
As shown in table 9, after adding Chinese herbal medicine in daily ration, Cholesterol Content in Egg Yolk significantly reduces, and along with the time of interpolation
Extend downward trend obvious.Test the 20th day, ii, III group cholesterol level of vitellus respectively than control group decline 12.67%,
10.94%, significant difference (P<0.05), group IV and each group of difference is not notable (P>0.05)；Test the 40th day, ii,
III, IV group cholesterol level of vitellus declines 20.50%, 15.72%, 13.97% than control group respectively, and difference is extremely notable (P < 0.01).
Ii, III, IV group cholesterol level of vitellus all declined than the 20th day at the 40th day, and control group but has substantially rising to become
Gesture.
Table 9 compound Chinese medicinal additive is on the impact of cholesterol in yolk
In yolk, the content of cholesterol mainly determines lipoprotein constituent in liver, and lipoprotein constituent is sweet with blood
Oil three ester contents are relevant, so serum cholesterol levels affects the content of cholesterol in yolk, therefore take effective way to drop
Low Egg cholesterol amount is significant.Experimental result shows, after adding Chinese herbal medicine in daily ration, in yolk, cholesterol contains
Amount significantly reduces, and along with the prolongation downward trend of the time of interpolation is obvious.Testing the 20th day, ii, III group yolk courage are solid
Alcohol content have dropped 12.67%, 10.94% than control group respectively, and significant difference (P < 0.05), group IV does not shows with each group of difference
Write (P > 0.05)；Testing the 40th day, I, II, III group cholesterol level of vitellus have dropped 20.50% than control group respectively,
15.72%, 13.97%, difference is extremely notable (P < 0.01).In this test mesolecithal, cholesterol level reduces, in this and serum
The result that cholesterol level also reduces is consistent.Chinese herbal medicine group cholesterol level of vitellus is at the 40th day all than the 20th day
Declining, control group but has obvious ascendant trend, may be with along with the increase of age in days, and the degradation capability of cholesterol is declined by laying hen
Relevant.
By embodiment 1 or 2, the preparation-obtained feed addictive of embodiment 3 or 4 is when laying hen feeds, acquired by it
Technique effect is similar with the technique effect in the embodiment of the present invention 6, and this shows that feed addictive of the present invention is laid eggs for laying hen
Rate and quality of laying eggs have and are obviously improved effect, are suitable in layer breeding using.And due to feed addictive of the present invention
In containing plurality of Chinese component, and without any antibiotic or other chemical substances, obtained egg green safety and nothing
Any drug resistance produces.
Claims (6)
1. the feed addictive improving Layer Production Performance, it is characterized in that, described feed addictive is prepared by following traditional Chinese medicinal components: sub-Soviet Union 8-12 part, giant knotweed 15-19 part, fleece-flower root 13-17 part, Chinese yam 10-20 part, Radix Astragali 5-9 part, rheum officinale 11-17 part, honey-fried licorice root 12-16 part, date 10-20 part, Semen sesami nigrum 10-20 part.
2. the feed addictive as described in right 1, it is characterized in that, described feed addictive is prepared by following traditional Chinese medicinal components: sub-Soviet Union 10 parts, giant knotweed 17 parts, the fleece-flower root 15 parts, Chinese yam 15 parts, the Radix Astragali 7 parts, rheum officinale 14 parts, honey-fried licorice root 14 parts, 15 parts of date, Semen sesami nigrum 15 parts.
3. the method preparing the arbitrary described feed addictive of claim 1-2, it is characterised in that described preparation method comprise the steps: by simple through 105 DEG C dries, pulverize, mistake 80 mesh sieves, mix according to traditional Chinese medicinal components proportioning, to obtain final product.
4. claim 1-2 arbitrary described feed addictive application in laying hen feeds.
Apply the most as claimed in claim 4, it is characterised in that described laying hen is HUANGHE ESTUARY black-bone chicken.
Apply the most as claimed in claim 5, it is characterised in that described feed addictive is 0.005-0.015:1 with the weight ratio of basal diet.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1483341A (en) * | 2002-09-19 | 2004-03-24 | 田洪沛 | Natural nutrient for egg-laying poultry and use method thereof |
| CN103156095A (en) * | 2013-04-02 | 2013-06-19 | 山东新希望六和集团有限公司 | Mixed feed of young broiler chickens and preparation method of feed |
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2016
- 2016-05-18 CN CN201610331249.XA patent/CN105876253A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1483341A (en) * | 2002-09-19 | 2004-03-24 | 田洪沛 | Natural nutrient for egg-laying poultry and use method thereof |
| CN103156095A (en) * | 2013-04-02 | 2013-06-19 | 山东新希望六和集团有限公司 | Mixed feed of young broiler chickens and preparation method of feed |
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Application publication date: 20160824 |