CN105875592B - One kind killing Neurospora composite drug - Google Patents

One kind killing Neurospora composite drug Download PDF

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Publication number
CN105875592B
CN105875592B CN201610308890.1A CN201610308890A CN105875592B CN 105875592 B CN105875592 B CN 105875592B CN 201610308890 A CN201610308890 A CN 201610308890A CN 105875592 B CN105875592 B CN 105875592B
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fungicide
neurospora
added
composite drug
killing
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CN105875592A (en
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李程碑
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Shaanxi Chemical Research Institute Co ltd
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Shaanxi Research Design Institute of Petroleum and Chemical Industry
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/34—Nitriles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • A01N47/44—Guanidine; Derivatives thereof

Abstract

The present invention relates to one kind killing Neurospora composite drug, by fungicide A, fungicide B, fungicide C, fungicide D, aid antibacteria agent and solvent are prepared, wherein fungicide A is by 2, 2 dibromo, 3 nitrilo- propionamide, Bronopol, C5H6Br2N2O2, one or more material compositions in bromochloroin, fungicide B is by poly (hexamethylene) hydrochloride, double decyl ammonium chlorides, new clean youngster one or both of is gone out material composition, fungicide C is by 3 iodine, 2 propynyl butylcarbamate, octylisothiazolinone, 4, one or both of 5 dichloro octylthiazol quinoline ketone material composition, fungicide D is biguanide spicy acid salt, aid antibacteria agent is made of anhydrous sorbitol list caprylate and/or Monooctamoin.The present invention is compound using the fungicide of a variety of antimicrobial spectrums complementation, and sterilization is quick and does not recur persistently, and toxicological security is good, building performance strong, and Neurospora effect is killed using with good in cultivating edible.

Description

One kind killing Neurospora composite drug
Technical field
The invention belongs to the preparing technical field of cultivating edible chemical anti mildew agent, it is related to one kind and kills Neurospora with compound Medicament.
Background technology
Neurospora (Neurospora) be also Neurospora sp, is commonly called as red mould.Growth of fusarium initial stage be in villiform, white or Grey, sprawl growth, branch.Neurospora be distributed widely in nature soil and grass tannin plant on, especially in corncob, cotton Easily occur on seed shell, conidium floats everywhere in air, is mainly propagated and is endangered with conidium, is high temperature season hair Raw most important miscellaneous bacteria.
The spore germination of the bacterium, mycelial growth rate are exceedingly fast, and especially aerial hyphae (also crying sporogenous hyphae) is indomitable strong, It can be pierced by the joint filling material of strain, rack strain bag, the great conidium group of forming quantity, there is the same day to sprout, the next day produce The characteristic of spore high speed breeding.Within the temperature range of 20~30 DEG C, on slant medium, diel can cover with entire test tube. On sawdust and cotton seed hulls compost, sprawling is rapid, and transmissibility is strong, it is only necessary to can reach physiological maturity, bacterium colony within 1~3 day Position and its periphery can be formed quickly pollutes again, its harmfulness mainly inhibits mycelia growth to cause edible fungus cluster body dead It dies, it is very harmful to Edible Fungi, the destructive infection of bacterium bag in entire mushroom shed can be caused when serious, is commonly called as " edible mushroom Cancer ".Entire production site has all been covered with the spore of Neurospora red after 3 days, and bacterium bag is difficult to thoroughly remove once pollution, Chang Yin Play by the gross that strain or bacterium bag scrap and cause crushing loss.
This field does not have specific medicament for Neurospora at present, and several Neurospora medicaments that kill available on the market are through using effect Fruit is unsatisfactory, and most of using the prodigious pesticide of toxicity, such as Bravo, thiophanate methyl, methylpartricin sodium laurylsulfate, in interior Agriculture chemicals fungicide ingredient be difficult degradation, prodigious security risk is caused to edible mushroom.In addition, it is previous also once had using formaldehyde, The stifling mode such as potassium permanganate, naphthalene system fungicide and acetic acid kills Neurospora, but effect is limited.
Chinese patent application CN03149881.7 discloses a kind of medicament of prevention Neurospora, and the medicament is by methyl Tobe Tianjin, methylpartricin sodium laurylsulfate, streptomysin, Wan Xiaoling, Bravo and pure water mixing are made, since components Most used is agricultural bactericidal Agent, be more toxic, degradability it is poor.
Medicaments and its preparation, use of the Chinese patent application CN201510586320.4 more particularly to a kind of prevention Neurospora Method.The medicament of the prevention Neurospora includes rice vinegar, triacontanol, methyl α-naphthyl acetate and water, although its toxicity is little, effect has Limit.
Chinese patent application CN92104223.X is related to a kind of manufacturing method of special pesticide for diseases and insect pests of edible mushroom, the sterilization Agent is formed by host agent and additive compound, and weight ratio shared by host agent is 20~75%, and component includes benzimidazolyl-2 radicals-Ji Anjijia Sour methyl esters, 2- (thiazole-4-yl) benzimidazoles and sulphur, still majority used is disinfectant use in agriculture, is more toxic, use is not square Just.
Chinese patent application CN94105892.1 disclose it is a kind of be considered as the Trichoderma to edible mushroom, Neurospora, Green mold bacterium and living contaminants have the disinfectant of the high-efficiency broad spectrum of preferable prevention and inhibiting effect, using Chlorothalonil Based on disinfection sanitizer sodium dichloro cyanurate, addition is compounded with various trace elements.But said preparation equally exists poison Safety issue is managed, and since solubility of two kinds of medicaments in organic solvent and water is limited, prepares and use is also inconvenient.
Chinese patent application CN201010294919.8 also once disclosed a kind of Neurospora fungicide, the Neurospora fungicide It is by Liquid Culture Pseudomonas putida strain, bacterium solution centrifuging and taking supernatant obtains.Though this Neurospora fungicide has sterilization The features such as effect is good, safe, but its operational feasibility is still limited by various factors with practicability.
Invention content
It is an object of the invention to be solved to problem of the existing technology, provide it is a kind of formula rationally, cost performance Height, toxicity safety, operation possibility and with using effect it is good efficiently kill Neurospora composite drug.
The active ingredient for killing Neurospora composite drug provided for realizing foregoing invention purpose is by following weight hundred Divide the raw material composition of ratio：
The fungicide A is selected from one or more of following substances：Bis- bromo- 3- nitrilo-s propionamides of 2,2- (DBNPA), Bronopol, C5H6Br2N2O2, bromochloroin；The fungicide B is selected from one or both of following substances：It is poly- Hexa-methylene guanidine hydrochloride, double decyl ammonium chlorides, new clean youngster are gone out；The one kind or two of the fungicide C in following substances Kind：3-Iodo-2-propynyl butyl carbamate (IPBC), octylisothiazolinone (OIT), 4,5- dichloro octylthiazol quinoline ketone (DCOIT)；The fungicide D is biguanide spicy acid salt；The aid antibacteria agent is anhydrous sorbitol list caprylate and/or sweet Oily list caprylate；The solvent is one or both of following substances：Ethyl alcohol, 1,3 butanediols, 1,2 propylene glycol, poly- second two Alcohol, dipropylene glycol, ethylene glycol.
The preferred weight percent range of the raw material of the present invention for killing Neurospora composite drug, which is made, is：
The further technical solution of the present invention also resides in：The fungicide A is bis- bromo- 3- nitrilo-s propionyl of 2,2- Amine (DBNPA), fungicide B are poly (hexamethylene) hydrochloride (PHMB), and fungicide C is the iodo- 2-propynyl butylamino first of 3- Acid esters (IPBC), the fungicide D are biguanide spicy acid salt；The aid antibacteria agent is anhydrous sorbitol list caprylate； The solvent is the mixture of polyethylene glycol 200 and ethyl alcohol, and the weight ratio of polyethylene glycol 200 and ethyl alcohol is 2 in the mixture: 1。
Each component raw material of substance is respectively possessed in sterilization antiseptic composition in inventive formulation acts on following institute It states：
It is powerful quick oxidation disinfection effect, Strong oxdiative that fungicide A, which mainly selects oxidation disinfection substance, the mechanism of action, Property destroy composition microorganism protein；
It is by electrostatic force, hydrogen bond force and surface that fungicide B, which mainly selects cationic germicide substance, the mechanism of action, The effects that active agent molecule is combined with the intermolecular hydrophobic part of microprotein, adsorbs electronegative microorganism, causes micro- Biological growth is suppressed and dead；Its hydrophobic alkyl can also be acted on the hydrophilic group of bacterium simultaneously, changed the permeability of film, then sent out Raw lysis, destroys eucaryotic cell structure, causes the dissolving and death of cell；
Fungicide C, which is mainly selected, inhibits mould substance, and the mechanism of action is that the antibacterial activity of IPBC passes through to the micro- life of target The cell of object carries out oxidation to complete, make mercapto groups oxidation, tyrosine iodate and lose protein active；Octyl isothiazole Quinoline ketone (OIT), 4,5- dichloros octylthiazol quinoline ketone (DCOIT) strand and microorganism cell membrane surface anion binding or with Sulfydryl reacts, to destroy the composition of microbial cell film；
Fungicide D mainly selects biguanide spicy acid salt, the mechanism of action be can quick solidification microprotein, make microorganism Enzyme system is denaturalized, and microorganism purpose is killed to reach；
Aid antibacteria agent mainly selects prepared by natural material to have sterilization and surface-active substance, and the mechanism of action is： The interfacial tension between medicament active ingredient and Neurospora cell is reduced, medicament is assisted to enter cell interior or closely and cell wall Contact plays a role, while itself also has killing fungus effect.
Selected mixed solvent has the good dissolution taken into account to above-mentioned each component.
It includes processing step below to be used to prepare the method for killing Neurospora composite drug：
1, the solvent for weighing required solvent total weight 30%~40% in formula is added in a container, is added with stirring Fungicide D, dissolving are spare；
2, the solvent for weighing required solvent total weight 40%~50% in formula is added in another container, is added with stirring Fungicide A dissolving, be eventually adding fungicide B stir evenly it is spare；
3, remaining solvent is added in third container, is added with stirring aid antibacteria agent, added again after mixing Enter fungicide C stirring and dissolvings, it is spare；
4, three kinds of solution of above-mentioned preparation are mixed 25~45 minutes, obtain the present invention kills Neurospora composite medicine Agent.
Compared with prior art, beneficial effects of the present invention are as described below.
One, the present invention is compound using the complementary different fungicide of various sterilization spectrum, it is made to play classic biocidal efficacies, And realize have complementary advantages according to the antimicrobial spectrum of related fungicide, it can take into account quick sterilization through scientific compatibility formation and long-time is antibacterial The compound disinfectant of the better adaptability of efficiency really plays the effect of " learning from other's strong points to offset one's weaknesses, bring out the best in each other ".This kills Neurospora use The toxicity of composite drug is low, biodegradable, not only has apparent prevent to miscellaneous bacterias such as Neurospora, aspergillus niger, penicillium funiculosums Effect, and the Neurospora to having grown and other moulds have strong killing effect, product to be tried by a large amount of microbe research It tests and large-scale field application test, effect is fine, is imitated using with the good Neurospora that kills especially in cultivating edible Fruit.In addition, the present invention is compound by different fungicide, additive amount can be also reduced while improving drug effect, it especially will be several Effect is especially pronounced when the fungicide of different role mechanism is used cooperatively.
Two, of the present invention to kill Neurospora with composite drug be solution-type, easy to use, and when use adds water stirring to become breast Liquid is now matched and is limited the use of, infiltrates, and spray is shone, injection.
Three, the compatibility of the present invention for killing Neurospora composite drug is good.In view of some are killed with oxidisability in the market Microbial inoculum (such as sodium dichloro cyanurate) though based on to kill Neurospora medicament sterilization speed fast, can be answered again after general a few houres Hair, or there is situation only effective and invalid to Neurospora to green mold bacterium, the present invention ties quick sterilization and persistently sterilization Conjunction combines medicament infiltration and surface layer sterilization, and germicidal efficiency higher, the duration is longer, and thalline does not recur growth after sterilization.
Four, acid-base value (pH value) scope of application of the present invention for killing Neurospora composite drug is larger, and bactericidal effect is not By water quality impact.
Five, the preparation method of the present invention for killing Neurospora composite drug is simple, and production cost is low, it is easy to accomplish scale Metaplasia is produced.
Six, the active component of the present invention killed in Neurospora composite drug is all largely that industry especially daily use chemicals are produced The anticorrosion and bactericidal agent that product use, toxicity have the greenization of edible mushroom cultivation important well below disinfectant use in agriculture Meaning.
Specific implementation mode
Embodiment 1
A kind of preparation method of the embodiment of the present invention for killing Neurospora composite drug includes processing step below：
1, it weighs 25.66 kilograms of absolute ethyl alcohol to be added to the container, is added with stirring 3 kilograms of biguanide spicy acid salts, dissolve, it is standby With；
2, it weighs 36.0 kilograms of polyethylene glycol to be added in another container, is added with stirring 7 kilograms of 2,2-, bis- bromo- 3- nitrilo-s Propionamide (DBNPA) dissolve, be eventually adding 6 kilograms of poly (hexamethylene) hydrochlorides (PHMB) stir evenly it is spare；
3, it weighs 15.5 kilograms of polyethylene glycol to be added in another container, is added with stirring 3 kilograms of anhydrous sorbitol list octanoic acids 3-Iodo-2-propynyl butyl carbamate (IPBC) 4 kilograms of stirring and dissolvings are added in ester after mixing, spare；
4, three kinds of solution of above-mentioned preparation are mixed 30 minutes, obtain the present invention kills Neurospora composite drug.
Each component raw material is as follows in respective possessed effect in Neurospora composite drug of killing in above example formula It is described.
2,2-Dibromo-3-nitrilopropionamide (DBNPA) activity is very high and have a wide spectrum kills microorganism ability, sterilization speed Degree is fast, and the time, which is short to a few minutes, to take effect, its molecule can readily penetrate through the cell membrane of microorganism and act on certain egg White gene makes the normal redox of cell terminate, and leads to cell death and is removed with former attachment point, in addition, DBNPA is in nature It is easily degraded in environment.
Poly (hexamethylene) hydrochloride (PHMB) is the efficient germicide for having cationic characteristic.PHMB is to bacterium, fungi There is killing ability with yeast, it is and thin by acting on bacterium, fungi, the cell wall system of algae and/or cell membrane system After birth phosphatide cpd combines, and the rapid film system for puncturing microorganism makes thalline intracellular organic matter outflow, and inhibit certain attachments In the ferment activity of cell, to reach antibacterial and mouldproof effect, the hypotoxicity of PHMB is also generally satisfactory.
3-Iodo-2-propynyl butyl carbamate (IPBC) is the compound of environmentally friendly killing fungus, can effectively be killed It goes out mould, saccharomycete, algae and bacterium, has special efficacy to fungi.The killing fungus effect of IPBC is largely effective, has been widely used in It is one of the fungicide being most widely used in the world at present in various commercial waterborne products.
Biguanide spicy acid salt is that a kind of novel wide-spectrum bactericide gradually draws because of the advantages that its toxicity is relatively low, antibiotic property is good Play the attention of people.Especially there is notable control effect to the fungi propagated by soil and seeds or bacterial disease, at this It is also used for the fungicidal effect of synergy IPBC in invention formula.
Anhydrous sorbitol list caprylate (also referred to as the smooth caprylate of sorb) is that a kind of new multi-functional of research and develop in recent years adds Add agent, be that sorbierite sloughs the product being esterified with a molar after a molecular water, it has the other fungicide of synergy Effect, and the interfacial tension between medicament and microbial cell can be reduced, so that other fungicide is easily entered in microbial cell.It loses Water sorbierite list caprylate itself has certain sterilizing ability, some surfactants is overcome to imitate the reduction of fungicidal activity It answers.
The mixture of polyethylene glycol and ethyl alcohol is added in above-mentioned preferred composition of raw materials as solvent, has to each other groups Divide the good dissolution taken into account.
The Neurospora that kills that embodiment 1 obtains is subjected to mould proof level estimate with composite drug, in test process, by Neurospora And aspergillus niger, penicillium funiculosum are inoculated on culture medium, and the sample after inoculation is placed under defined temperature and humidity and is cultivated, timing Ventilation finally uses the growth and development situation of magnifying glass spore, mycelia and bacterium colony from a front surface and a side surface, provides fungi-growth grade and sentence Fixed (table 1).Prevention of the Neurospora composite drug to Neurospora and aspergillus niger, penicillium funiculosum is killed by the visible present invention of test Effect is considerably beyond commercial product.
1 fungi-growth grade test result of table
Embodiment 2 (comparison of embodiment 1)
In embodiment 1, the addition of biguanide spicy acid salt, IPBC, anhydrous sorbitol list caprylate is replaced by PHMB, i.e., Kill Neurospora composite drug by 16% PHMB (weight) and 7% DBNPA (weight) and mixed solvent form.
Embodiment 3 (comparison of embodiment 1)
In embodiment 1, biguanide spicy acid salt is kept, the addition of IPBC, anhydrous sorbitol list caprylate are constant, are free of PHMB and DBNPA, the two are replaced by the mixed solvent of same weight.
Embodiment 4 (comparison of embodiment 1)
In embodiment 1, other components are constant, only the Tween 80 surfactant of anhydrous sorbitol list caprylate same amount Instead of.
The training of Neurospora composite drug and commercially available two kinds of medicaments for covering with Neurospora is killed by what is obtained in above-mentioned each example It supports and does killing test on base, concentration is 0.3%, is as a result provided in table 2.As it can be seen that the present invention's kills Neurospora composite drug (in fact Apply example 1) have and kills Neurospora effect well.
Table 2, embodiment and the commercially available bactericidal effect for killing Neurospora medicament
Time (hour) Embodiment 1 Embodiment 2 Embodiment 3 Embodiment 4 Commercially available product 1 Commercially available product 2
0.2 - - + +— — +—
12 - - + +— — +—
24 - + +— - +— +—
36 - + +—— - +— +
48 - ++ +—— - ++ ++
72 - +++ +—— +— ++ +++
96 - ++++ +— +— +++ ++++
In table 2, the long bacterium of "+"=minute quantity；" ++ "=a small amount of long bacterium；" +++ "=a large amount of long bacterium；" ++++"=cover with bacterium； "-"=not long bacterium；"+- "=have inhibiting effect to bacterium；"+--- "=have bacterium compared with high inhibition effect, but cannot kill completely It goes out.

Claims (2)

1. one kind killing Neurospora composite drug, which is characterized in that the active ingredient of the composite drug is by following weight percentage Raw material composition：
The fungicide A is 2,2-Dibromo-3-nitrilopropionamide, and fungicide B is poly (hexamethylene) hydrochloride, fungicide C is 3-Iodo-2-propynyl butyl carbamate, and the fungicide D is biguanide spicy acid salt；The aid antibacteria agent is Anhydrous sorbitol list caprylate；The solvent is the mixture of polyethylene glycol 200 and ethyl alcohol, polyethylene glycol in the mixture 200 with the weight ratio of ethyl alcohol be 2:1.
2. a kind of being used to prepare the method for killing Neurospora composite drug described in claim 1, which is characterized in that including below Processing step：
2.1 solvents for weighing required solvent total weight 30%~40% in formula are added in a container, are added with stirring and are killed Microbial inoculum D, dissolving are spare；
2.2 solvents for weighing required solvent total weight 40%~50% in formula are added in another container, are added with stirring and are killed Microbial inoculum A dissolving, be eventually adding fungicide B stir evenly it is spare；
2.3 are added to remaining solvent in third container, are added with stirring aid antibacteria agent, add kill after mixing Microbial inoculum C stirring and dissolvings, it is spare；
2.4 three kinds of solution of above-mentioned preparation are mixed 25~45 minutes, and obtain the present invention kills Neurospora composite drug.
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