CN105853907A - Traditional Chinese medicinal composition and preparation method of depression resolving pills - Google Patents

Traditional Chinese medicinal composition and preparation method of depression resolving pills Download PDF

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Publication number
CN105853907A
CN105853907A CN201610224619.XA CN201610224619A CN105853907A CN 105853907 A CN105853907 A CN 105853907A CN 201610224619 A CN201610224619 A CN 201610224619A CN 105853907 A CN105853907 A CN 105853907A
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depression
pills
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杨海娟
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
    • A61K36/076—Poria
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/232—Angelica
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/233—Bupleurum
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    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/484—Glycyrrhiza (licorice)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/72—Rhamnaceae (Buckthorn family), e.g. buckthorn, chewstick or umbrella-tree
    • A61K36/725—Ziziphus, e.g. jujube
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
    • A61K36/8967—Lilium, e.g. tiger lily or Easter lily
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/906—Zingiberaceae (Ginger family)
    • A61K36/9066—Curcuma, e.g. common turmeric, East Indian arrowroot or mango ginger
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    • A61K9/00—Medicinal preparations characterised by special physical form
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    • A61K9/2004—Excipients; Inactive ingredients
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    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying

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Abstract

The invention relates to a traditional Chinese medicinal composition and a preparation method of depression resolving pills. The traditional Chinese medicinal composition is prepared through processing white peony root, radix bupleuri, Chinese angelica root, Radix Curcumae Aromaticae, Poria cocos, lily, Cortex Albiziae, licorice root, wheat and jujubes. The pills have the efficacy of coursing liver, resolving depression, nourishing heart and tranquilizing mind, and are mainly used to treat sternal rib turgor, depression uneasiness, vexation and palpitation, testiness, insomnia and dreaminess induced by liver-qi stagnation and malaise. Compared with depression resolving pills in the prior art, the depression resolving pills provided by the invention has the advantages of advanced technology, substantially improved clinic pharmacodynamic test effect, high bioacvailability, and no toxic or side effects.

Description

A kind of depression alleviating pills Chinese medicine composition and preparation method thereof
Technical field
The present invention relates to a kind of depression alleviating pills Chinese medicine composition and preparation method thereof, belong to pharmaceutical technology field.
Background technology
Depression is caused by multiple uncertain reason, low as main clinical characteristics with notable and lasting mental state, is the world On one of the disturbance of emotion mental illness the most easily disabled, be listed in the big mental disease of the first in the world, bring to the life of people The most painful, the serious health that have impact on people.The treatment of this disease is mainly adjusted by doctor trained in Western medicine with antidepression, antianxiety etc. at present Joint nervous system medicine, all can only relief of symptoms, have also has the most serious toxic and side effect.Chinese patent drug in terms for the treatment of depression is the most very Many, but curative effect is general mostly, therefore, develops a kind of cure rate high, the recurrence after healing can be avoided, safety, the treatment having no side effect The medicine of depressive illness, it appears the most urgent.Prior art, Chinese patent publication discloses (publication number on March 26th, 2003 For the CN 1404864A) prescription of the Chinese medicine depression alleviating pills of depression " treatment " and technique, the present inventor through years of researches, Find that the preparation prepared by its technique exists: curative effect is undesirable, and cure rate is low, easily recurs；Technique is relatively thick, active constituent content The defect such as low.For solving the defect existing for above-mentioned prior art, in the time of last decade, we enrich by excavating motherland Traditional Chinese medicine resource, in conjunction with substantial amounts of Study on clinical pharmacodynamics, invented a kind of depression alleviating pills Chinese medicine composition and preparation method thereof, This product " depression alleviating pills " is on the basis of its technique, is groped by substantial amounts of experiment, find by its technique " Poria cocos, Lily, cortex albiziae, Radix Glycyrrhizae, wheat, date boiling " change into " Poria cocos, lily, cortex albiziae, Radix Glycyrrhizae, wheat, big Jujube adds 40% ethanol and decocts " after, clinical pharrnacokinetics experiment effect significantly improves, and we and concentration of alcohol used to ethanol decoction enter Having gone in-depth study, found to compare the alcohol extract with 95%, 75% with 40% alcohol extract effective, we are according to new technology system Become pill, and disclosed (Publication No.: CN 1404864A) the Chinese medicine depression alleviating pills of depression " treatment " according to Chinese patent Prescription and the pill that is prepared from of technique compare, its clinical pharrnacokinetics experiment effect significantly improves, and technique is more reasonable, medicine Active constituent content is high, safe and without any side effects.
Summary of the invention
It is an object of the invention to provide a kind of depression alleviating pills Chinese medicine composition and preparation method thereof, for reaching above-mentioned purpose, the present invention adopts Technical scheme be:
1, bulk drug weight proportion:
Preparation method is: above ten tastes, takes radix bupleuri, Radix Angelicae Sinensis, the root of herbaceous peony, turmeric powder are broken into mixed powder, then by Poria cocos, lily, Cortex albiziae, Radix Glycyrrhizae, wheat, date add 40% ethanol and decoct 2 times, add 12 times amount for the first time, decoct 1.5 hours, pour out medicine Liquid is standby, and second time adds 10 times amount, decocts 1 hour, merges twice decocting liquid, filters, decompression recycling ethanol, is concentrated into Record the medicinal extract that relative density is 1.30-1.35 under conditions of 60 DEG C, make the water-bindered pill with the mixing of four traditional Chinese medicine powder, be dried and make 1000g Pill.
The present invention program is through inventor's repeatedly repetition test, in conjunction with substantial amounts of Study on clinical pharmacodynamics, has invented a kind of solution strongly fragrant Ball Chinese medicine composition and preparation method thereof, this product " depression alleviating pills " is in its (Publication No.: CN 1404864A) technique On the basis of, groped by substantial amounts of experiment, find in its technique is " Poria cocos, lily, cortex albiziae, Radix Glycyrrhizae, wheat, big Jujube boiling " change " Poria cocos, lily, cortex albiziae, Radix Glycyrrhizae, wheat, date add 40% ethanol and decoct " into after, clinical medicine Effect is learned experiment effect and is significantly improved, and we and concentration of alcohol used to ethanol decoction conduct in-depth research, and find to use 40% second Alcohol extracting ratio is effective with the alcohol extract of 95%, 75%, and we are made for pill according to new technology, open with according to Chinese patent The pill phase that the prescription of (Publication No.: CN 1404864A) " the Chinese medicine depression alleviating pills for the treatment of depression " and technique are prepared from Ratio, its clinical pharrnacokinetics experiment effect significantly improves, and technique is more reasonable, and effective ingredient content is high, safety and without any Toxic and side effect.
Depression alleviating pills (preparing according to the method for embodiment 1) the contrast Chinese patent prepared with the present invention below discloses (publication number For CN 1404864A) pill prepared by the Chinese medicine depression alleviating pills of depression " treatment ", its Pharmacodynamics is described:
One, the preparation of trial drug:
1, A group (preparation of depression alleviating pills of the present invention): prepare according to the method for the embodiment of the present invention 1.
2, B group for Chinese patent disclose (Publication No.: CN 1404864A) the Chinese medicine depression alleviating pills of depression " treatment " enforcement Pill prepared by example, specific as follows:
Preparation method: above ten tastes, takes radix bupleuri, Radix Angelicae Sinensis, the root of herbaceous peony, turmeric powder are broken into mixed powder, then by Poria cocos, lily, silk tree Skin, Radix Glycyrrhizae, wheat, date boiling 2 times, add water 12 times amount for the first time, decocts 1.5 hours, pours out liquid standby, Add water 10 times amount for the second time, decocts 1 hour, merges twice decocting liquid, filters, and concentrates, and records relatively under conditions of 60 DEG C Density is the medicinal extract of 1.30-1.35, makes the water-bindered pill with the mixing of four traditional Chinese medicine powder, is dried and makes 1000g pill.
3, C group (screening 1 group):
Preparation method: above ten tastes, takes radix bupleuri, Radix Angelicae Sinensis, the root of herbaceous peony, turmeric powder are broken into mixed powder, then by Poria cocos, lily, cortex albiziae, Radix Glycyrrhizae, wheat, date add 95% ethanol and decoct 2 times, add 12 times amount for the first time, decoct 1.5 hours, pour out liquid standby, Second time adds 10 times amount, decocts 1 hour, merges twice decocting liquid, filters, decompression recycling ethanol, is concentrated into the bar at 60 DEG C Record the medicinal extract that relative density is 1.30-1.35 under part, make the water-bindered pill with the mixing of four traditional Chinese medicine powder, be dried and make 1000g pill.
4, D group (screening 2 groups):
Preparation method: above ten tastes, takes radix bupleuri, Radix Angelicae Sinensis, the root of herbaceous peony, turmeric powder are broken into mixed powder, then by Poria cocos, lily, cortex albiziae, Radix Glycyrrhizae, wheat, date add 75% ethanol and decoct 2 times, add 12 times amount for the first time, decoct 1.5 hours, pour out liquid standby, Second time adds 10 times amount, decocts 1 hour, merges twice decocting liquid, filters, decompression recycling ethanol, is concentrated into the bar at 60 DEG C Record the medicinal extract that relative density is 1.30-1.35 under part, make the water-bindered pill with the mixing of four traditional Chinese medicine powder, be dried and make 1000g pill.
Two, process of the test and result
Experiment purpose: by depression alleviating pills group of the present invention, B group, C group and calmness, the hypnosis of D group pill, ease pain, regulate body temperature, The pharmacological experiment studies such as antidepression, contrast depression alleviating pills group of the present invention, B group, C group and D group, observe it The power of pharmacological action.
Test method: depression alleviating pills group of the present invention, B group, C group and the impact on spontaneous activity in mice of the D group；Shadow to mouse time for falling asleep Ring；Impact on mouse sleep time；Impact on mouse Radiant heat pain stimulus；Shadow to depression model mouse temperature Ring；Reserpine induction depression model mice is tested.
One, the impact on spontaneous activity in mice
Experiment material
1, animal: Kunming mouse, male and female have concurrently, body weight 18～22g.
2, medicine: depression alleviating pills group of the present invention, B group, C group, four dosage groups of D group.Medicine configures with distilled water on pretreatment, fills Stomach is administered.
Experimental technique
Kunming mouse 50, male and female half and half, body weight 18～22g, it is randomly divided into 5 groups, often group 10.Control group gavage consubstantiality Long-pending distilled water；Depression alleviating pills group of the present invention, B group, C group, D group gastric infusion 6.8g crude drug/kg respectively.Successive administration 5d, end After secondary administration 1h, mouse is put into photoelectric counter, measure mouse 5min activity number of times.Experimental result: be shown in Table 1
The table 1 impact on spontaneous activity in mice
* * P ＜ 0.01 compared with control group；* P ＜ 0.05；With depression alleviating pills group of the present invention than △ P ＜ 0.05.
Result shows: depression alleviating pills group of the present invention, B group, C group and D group can significantly reduce the Assay of spontaneous activity of mouse, the present invention Depression alleviating pills group has the difference of pole conspicuousness (P ＜ 0.01) compared with control group with B group；C group has significantly with D group compared with control group Sex differernce (P ＜ 0.05)；B group, C group, D group have significant difference (P ＜ 0.05) compared with depression alleviating pills group of the present invention.It is visible, Depression alleviating pills group of the present invention than B group, C group, D group sedation strong.
Two, the impact on mouse time for falling asleep
Experiment material
1, animal: Kunming mouse, male and female have concurrently, body weight 18～22g.
2, medicine: depression alleviating pills group of the present invention, B group, C group, four dosage groups of D group.Medicine configures with distilled water on pretreatment, fills Stomach is administered.
Experimental technique
Kunming mouse 50, male and female half and half, body weight 18～22g, it is randomly divided into 5 groups, often group 10.Control group gavage consubstantiality Long-pending distilled water；Depression alleviating pills group of the present invention, B group, C group, D group gastric infusion 6.8g crude drug/kg respectively.Successive administration 5d, often It 1 time, 1h lumbar injection yellow Jackets 50mg/kg after last administration, with mouse righting reflex loss as time for falling asleep, record Time for falling asleep.Experimental result: be shown in Table 2
The table 2 impact on mouse time for falling asleep
* * P ＜ 0.01 compared with control group；* P ＜ 0.05；With depression alleviating pills group of the present invention than △ P ＜ 0.05.
Result shows: depression alleviating pills group of the present invention, B group, C group and D group substantially shorten time for falling asleep, depression alleviating pills group of the present invention and B Group has the difference (P ＜ 0.01) of pole conspicuousness compared with control group；C group has significant difference (P ＜ compared with control group with D group 0.05)；B group, C group, D group have significant difference (P ＜ 0.05) compared with depression alleviating pills group of the present invention.Visible, solution of the present invention Strongly fragrant ball group than B group, C group, D group syngignoscism strong.
Three, the impact on mouse sleep time
Experiment material
1, animal: Kunming mouse, male and female have concurrently, body weight 18～22g.
2, medicine: depression alleviating pills group of the present invention, B group, C group, four dosage groups of D group.Medicine configures with distilled water on pretreatment, fills Stomach is administered.
Experimental technique
Kunming mouse 50, male and female half and half, body weight 18～22g, it is randomly divided into 5 groups, often group 10.Control group gavage consubstantiality Long-pending physiological saline；Depression alleviating pills group of the present invention, B group, C group, D group gastric infusion 6.8g crude drug/kg respectively.Successive administration 5d, After last is administered 1h, lumbar injection yellow Jackets 40mg/kg, the length of one's sleep of record mouse is (with righting reflex loss for falling asleep Time, is sleep time from righting reflex loss to recovery time).Experimental result: be shown in Table 3
The table 3 impact on mouse sleep time
* * P ＜ 0.01 compared with control group；* P ＜ 0.05；With depression alleviating pills group of the present invention than △ P ＜ 0.05.
Result shows: depression alleviating pills group of the present invention, B group, C group and D group can be obviously prolonged the length of one's sleep of mouse, and solution of the present invention is strongly fragrant Ball group has the difference of pole conspicuousness (P ＜ 0.01) compared with control group with B group；C group has conspicuousness poor with D group compared with control group Different (P ＜ 0.05)；B group, C group, D group have significant difference (P ＜ 0.05) compared with depression alleviating pills group of the present invention.Visible, this Invention depression alleviating pills group is stronger than the effect that B group, C group, the improvement of D group are slept.
Four, the impact on mouse Radiant heat pain stimulus
Experiment material
1, animal: Kunming mouse, male and female have concurrently, body weight 18～22g.
2, medicine: depression alleviating pills group of the present invention, B group, C group, four dosage groups of D group.Medicine configures with distilled water on pretreatment, fills Stomach is administered.
Experimental technique
Being fixed on by mouse in mouse cylinder, a light beam planoconvex lens is irradiated in rat-tail after focusing on, lower 1/3 intersection, measures and starts light The time that source to rat-tail swings rapidly, i.e. TFL, the also known as threshold of pain.First measure the Basic Pain Threshold value before mouse is administered, base Irritation or blunt animal that plinth pain threshold less than 3s or is more than 10s are rejected.Mouse after screening 50, male and female half and half, at random It is divided into 5 groups, often group 10.The distilled water of control group gavage same volume；Depression alleviating pills group of the present invention, B group, C group, D group fill respectively Stomach is administered 6.8g crude drug/kg.Successive administration 5d, every day 1 time, after last is administered 1h, carries out radiant heat stimulation by mouse.Record After being administered 1h, there is the time that whipping reacts, be the pain threshold after medication.Experimental result: be shown in Table 4
The table 4 impact on mouse Radiant heat pain stimulus
* * P ＜ 0.01 compared with control group；* P ＜ 0.05；With depression alleviating pills group of the present invention than △ P ＜ 0.05.
Result shows: depression alleviating pills group of the present invention, B group, C group and D group are remarkably improved the pain threshold of Radiant heat pain stimulus mouse, this Bright depression alleviating pills group has the difference (P ＜ 0.01) of pole conspicuousness compared with control group with B group；C group has aobvious with D group compared with control group Write sex differernce (P ＜ 0.05)；B group, C group, D group have significant difference (P ＜ 0.05) compared with depression alleviating pills group of the present invention.Can See, depression alleviating pills group of the present invention than B group, C group, D group analgesic activity strong.
Five, the impact on depression model mouse temperature
Experiment material
1, animal: Kunming mouse, male and female have concurrently, body weight 18～22g.
2, medicine: depression alleviating pills group of the present invention, B group, C group, four dosage groups of D group.Medicine configures with distilled water on pretreatment, fills Stomach is administered.
Experimental technique
Kunming mouse 50, male and female half and half, body weight 18～22g, it is randomly divided into 5 groups, often group 10.Control group gavage consubstantiality Long-pending distilled water；Depression alleviating pills group of the present invention, B group, C group, D group gastric infusion 6.8g crude drug/kg respectively.Successive administration 5d, often It 1 time, being administered pre-test mouse anus temperature in 5d, after last is administered 1h, lumbar injection reserpine 2.5mg/kg, in drug administration by injection Rear 3h surveys mouse anus temperature, record anus temperature change again.Experimental result: be shown in Table 5
The table 5 impact on depression model mouse temperature
* * P ＜ 0.01 compared with control group；* P ＜ 0.05；With depression alleviating pills group of the present invention than △ P ＜ 0.05.
Result shows: depression alleviating pills group of the present invention, B group, C group and D group can recover rapidly the body temperature that reserpine induced mice declines, this Invention depression alleviating pills group has the difference (P ＜ 0.01) of pole conspicuousness compared with control group with B group；C group has compared with control group with D group Significant difference (P ＜ 0.05)；B group, C group, D group have significant difference (P ＜ 0.05) compared with depression alleviating pills group of the present invention. Visible, depression alleviating pills group of the present invention than B group, C group, D group regulation body temperature effect strong.
Six, reserpine induction depression model mice experiment
Experiment material
1, animal: Kunming mouse, male and female have concurrently, body weight 18～22g.
2, medicine: depression alleviating pills group of the present invention, B group, C group, four dosage groups of D group.Medicine configures with distilled water on pretreatment, fills Stomach is administered.
Experimental technique
Kunming mouse 50, male and female half and half, body weight 18～22g, it is randomly divided into 5 groups, often group 10.Control group gavage consubstantiality Long-pending distilled water；Depression alleviating pills group of the present invention, B group, C group, D group gastric infusion 6.8g crude drug/kg respectively.Successive administration 5d, often Day 1 time, after last is administered 1h, lumbar injection reserpine 2.5mg/kg, holds up mouse after 1h.Mark according to ptosis degree, It is 1 point that eye closes 1/4, and it is 2 points that eye closes 1/2, and it is 3 points that eye closes 3/4, and fully closed is 4 points.Calculate ptosis degree antagonistic rate.Experiment knot Really: be shown in Table 6
Table 6 reserpine induction depression model mice is tested
* * P ＜ 0.01 compared with control group；* P ＜ 0.05；With depression alleviating pills group of the present invention than △ P ＜ 0.05.
Result shows: depression alleviating pills group of the present invention, B group, C group and D group can substantially suppress eyelid after mouse peritoneal injection reserpine Sag, improves ptosis degree antagonistic rate, and depression alleviating pills group of the present invention has the difference (P of pole conspicuousness compared with control group with B group ＜ 0.01)；C group has significant difference (P ＜ 0.05) compared with control group with D group；B group, C group, D group and solution of the present invention Strongly fragrant ball group has compared significant difference (P ＜ 0.05).Visible, depression alleviating pills group of the present invention is than B group, C group, the antidepression of D group Effect is strong.
Experimental result: depression alleviating pills group of the present invention, B group, C group and D group can significantly reduce the Assay of spontaneous activity of mouse；Substantially shorten Time for falling asleep；The length of one's sleep of mouse can be obviously prolonged；It is remarkably improved the pain threshold of Radiant heat pain stimulus mouse； The body temperature that reserpine induced mice declines can be recovered rapidly；Can substantially suppress eyelid after mouse peritoneal injection reserpine Sag, improve ptosis degree antagonistic rate.
Conclusion: depression alleviating pills group of the present invention than B group, C group, the calmness of D group, hypnosis, ease pain, regulate the pharmacology such as body temperature, antidepression make With by force, therefore, depression alleviating pills group of the present invention is than B group, C group, D group soothing liver-qi stagnation, and the function of mental-tranquilization is strong.
Toxicological experiment:
Acute toxicity testing result shows: by depression alleviating pills group Cmax of the present invention, maximum volume gastric infusion, in 24h continuously Being administered 3 times, every minor tick 4h, accumulation medicine total amount reaches 48g crude drug/kg, is equivalent to people's clinic and intends 137.7 times of consumption.Give After medicine in 7d, mouse is movable, take food, drain all normally, well-grown, and hair color light, its average body weight average is with test period Prolongation and increase.At 8d, post mortem every mouse visually observes the heart, liver, spleen, lung, kidney, brain, thymus gland, stomach, intestines Do not find color and paramophia Deng all, fail to measure LD50.Show that depression alleviating pills group of the present invention is without acute toxic reaction.
Long term toxicity test result shows: depression alleviating pills component of the present invention be basic, normal, high dosage be respectively 6,12,24g crude drug/kg/d, Being equivalent to the 17.2 of clinical dosage, 34.4,68.9 times, gastric infusion is after 12 weeks, general to animal of depression alleviating pills group of the present invention Situation, hematological indices, blood parameters are all without significantly impact, Systematic anatomy, organ coefficient and histopathological examination The most no abnormal pathological change.Drug withdrawal also has no substantially change for 2 weeks.Depression alleviating pills group of the present invention is in long term toxicity test, not Find that overt toxicity reaction and delayed toxicity react.Visible, depression alleviating pills group non-toxic reaction of the present invention, long-term prescription is safe and reliable.
Detailed description of the invention
1, the preparation of Chinese medicine composition pill of the present invention:
Prescription:
Preparation method is:
Above ten tastes, take radix bupleuri, Radix Angelicae Sinensis, the root of herbaceous peony, turmeric powder are broken into mixed powder, then by Poria cocos, lily, cortex albiziae, Radix Glycyrrhizae, Wheat, date add 40% ethanol and decoct 2 times, add 12 times amount for the first time, decoct 1.5 hours, pour out liquid standby, for the second time Add 10 times amount, decoct 1 hour, merge twice decocting liquid, filter, decompression recycling ethanol, be concentrated into and survey under conditions of 60 DEG C Obtaining relative density is the medicinal extract of 1.30-1.35, makes the water-bindered pill with the mixing of four traditional Chinese medicine powder, is dried and makes 1000g pill.

Claims (1)

1. the preparation method of a depression alleviating pills Chinese medicine composition, it is characterised in that the bulk drug weight proportion of described Chinese medicine composition is:
Preparation method is:
Above ten tastes, take radix bupleuri, Radix Angelicae Sinensis, the root of herbaceous peony, turmeric powder are broken into mixed powder, then by Poria cocos, lily, cortex albiziae, Radix Glycyrrhizae, Wheat, date add 40% ethanol and decoct 2 times, add 12 times amount for the first time, decoct 1.5 hours, pour out liquid standby, for the second time Add 10 times amount, decoct 1 hour, merge twice decocting liquid, filter, decompression recycling ethanol, be concentrated into and survey under conditions of 60 DEG C Obtaining relative density is the medicinal extract of 1.30-1.35, makes the water-bindered pill with the mixing of four traditional Chinese medicine powder, is dried and makes 1000g pill.
CN201610224619.XA 2016-04-12 2016-04-12 Traditional Chinese medicinal composition and preparation method of depression resolving pills Pending CN105853907A (en)

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CN109289009A (en) * 2018-12-10 2019-02-01 河南泰丰生物科技有限公司 A kind of Chinese medicine composition and its preparation method and application

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CN1404864A (en) * 2002-10-27 2003-03-26 郑州豫密药业股份有限公司 Chinese medicine for treatment of depression
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* Cited by examiner, † Cited by third party
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CN109289009A (en) * 2018-12-10 2019-02-01 河南泰丰生物科技有限公司 A kind of Chinese medicine composition and its preparation method and application

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