CN101884756B

CN101884756B - Pharmaceutical composition with functions of expelling wind and removing dampness and activating collaterals and relieving pain, preparation method, preparation and application thereof

Info

Publication number: CN101884756B
Application number: CN 200910027588
Authority: CN
Inventors: 萧伟; 丁岗; 曹亮
Original assignee: Jiangsu Kanion Pharmaceutical Co Ltd
Current assignee: Jiangsu Kanion Pharmaceutical Co Ltd
Priority date: 2009-05-12
Filing date: 2009-05-12
Publication date: 2013-12-25
Anticipated expiration: 2029-05-12
Also published as: CN101884756A

Abstract

The invention relates to a pharmaceutical composition with functions of expelling wind and removing dampness, relaxing tendons and activating collaterals and warming meridians and relieving pain, which is characterized by being prepared from the following Chinese traditional medicine raw materials in parts by weight: 0.1-15 parts of galangal, 0.1-15 parts of clove, 0.1-15 parts of angelica, 0-30 parts of scandent schefflera root and 0-30 parts of semiliquidambar cathayensis. The invention also discloses a preparation method, a preparation and an application of the pharmaceutical composition. The pharmaceutical composition of the invention has good safety and obvious effects on relieving pain and resisting inflammation, and is suitable for patients with anemofrigid-damp arthralgia discriminated by the traditional Chinese medical science.

Description

The pharmaceutical composition of expelling wind and removing dampness, activating collaterals to relieve pain and method for making, preparation and purposes

Technical field

The present invention relates to a kind of pharmaceutical composition, particularly a kind of pharmaceutical composition with expelling wind and removing dampness, relaxing muscles and tendons and activating QI and blood in the collateral, antalgic effect that Chinese medicine is raw material of take; The invention still further relates to preparation method, preparation and the purposes of this pharmaceutical composition.

Background technology

Traditional Chinese medical science arthromyodynia refers to that the limbs meridians are the evil institute of wind, cold, wet obturation, causes that QI and blood is obstructed, obstruction of meridians and collaterals, cause muscle, joint, muscles and bones generation pain, miserable, numb, weighing, joint stuffiness, even the clinical disease of joint swelling deformation.Primary disease is equivalent to rheumatism, rheumatic arthritis, rheumatoid arthritis, ankylosing spondylitis, osteoarthritis, hyperosteogeny, the waist of doctor trained in Western medicine and asks the diseases such as dish is outstanding, postpartum apoplexy.

This disease is a kind of with the healthy energy virtual loss, deficiency of the liver and kindey is this, wind, cold, damp impatency joint, meridians, reduce phlegm into for a long time the stasis of blood, muscle and tendon injury erosion bone is that target is chronic, carry out property, affecting conditions, impose expelling wind and removing dampness with card more, dispelling cold by warming the meridian, removing obstruction in the collateral to relieve pain, dissipating phlegm and resolving masses, benefiting vital QI and blood, the methods such as nourishing the liver and kidney, because sickness rate is higher, people are exploring always, study this sick Therapeutic Method, but existing Chinese medicine preparation or because of effect clear, or because of toxicity too large, or because of preparation too thick, still can not satisfying the market to can effectively treating the demand of traditional Chinese medical science arthromyodynia Chinese patent medicine.

Summary of the invention

Technical problem to be solved by this invention be to provide a kind of compatibility rationally, have no side effect, there is the pharmaceutical composition of expelling wind and removing dampness, relaxing muscles and tendons and activating QI and blood in the collateral, antalgic effect.

Another technical problem to be solved by this invention has been to provide the multiple preparation method with pharmaceutical composition of expelling wind and removing dampness, relaxing muscles and tendons and activating QI and blood in the collateral, antalgic effect as above.

Another technical problem to be solved by this invention has been to provide the have pharmaceutical composition of expelling wind and removing dampness, relaxing muscles and tendons and activating QI and blood in the collateral, antalgic effect or the pharmaceutical preparation that preparation method as above is made as above.

Pharmaceutical composition of the present invention can be for the treatment of rheumatic arthritis, rheumatoid arthritis, numb limbs and tense tendons, back cold type of pain, scapulohumeral periarthritis, neck-shoulder pain, lumbago and leg pain, sciatica, trigeminal neuralgia, hyperosteogeny, prolapse of lumbar intervertebral disc, postpartum apoplexy etc.

Technical problem to be solved by this invention is to realize by following technical scheme.The present invention is the pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, is characterized in, it is to be made by the raw material of Chinese medicine of following weight proportion,

Rhizoma Alpiniae Officinarum 0.1～15; Flos Caryophylli 0.1～15; Radix Angelicae Sinensis 0.1～15;

Radix Schefflerae Arboricolae 0～30; Pterospermi Heterophylli 0～30.

Above-described pharmaceutical composition, the preferred weight proportioning of each raw material of Chinese medicine is:

Rhizoma Alpiniae Officinarum 0.5～6; Flos Caryophylli 0.5～3; Radix Angelicae Sinensis 0.5～9;

Radix Schefflerae Arboricolae 0～20; Pterospermi Heterophylli 0～20.

In above-described drug regimen composition formula of the present invention, Rhizoma Alpiniae Officinarum, Flos Caryophylli and Radix Angelicae Sinensis 3 flavor raw materials form substantially square; Radix Schefflerae Arboricolae, Pterospermi Heterophylli 2 flavor raw materials are optional raw materials; Any in optional raw material of 2 flavors or 2 kinds can carry out prescription with the above-mentioned 3 basic sides of flavor; When the weight proportion in optional raw material is selected " 0 ", represent in prescription and do not contain this raw material.

Technical problem to be solved by this invention can also realize by following technical scheme.Above-described pharmaceutical composition, be characterized in, it is to be made by the raw material of Chinese medicine of following weight proportion,

Radix Schefflerae Arboricolae 0.1～30 and Pterospermi Heterophylli 0.1～30.

The pharmaceutical composition of above-described expelling wind and removing dampness, activating collaterals to relieve pain, the preferred weight proportioning of each raw material of Chinese medicine is:

Rhizoma Alpiniae Officinarum 0.5～6; Flos Caryophylli 0.5～3; Radix Angelicae Sinensis 0.5～9

Radix Schefflerae Arboricolae 0.5～20; With/or Pterospermi Heterophylli 0.5～20.

In the pharmaceutical composition of above-described expelling wind and removing dampness, activating collaterals to relieve pain, described Radix Schefflerae Arboricolae has another name called Radix Schefflerae Arboricolae (Caulis et Folium Schefflerae Arboricolae), derive from Araliaceae Schefflera (Schefflera) various plants, mainly contain Radix Schfflerae kwangisensis S.kwangsiensis Merr.Ex Li, spend schefflera S.leacantha Vig in vain, Schefflera venulosa (Wight et Arn.) Harms S.venulosa Wight et Arn., schefflera S.octophylla Harms, ball order schefflera S.glomerulataL. and schefflera arboricola S.arboricola Hayata etc.

At above-described expelling wind and removing dampness, in the pharmaceutical composition of activating collaterals to relieve pain, described Pterospermi Heterophylli is Sterculiaceae Folium potentillae velutinae (Radix Potentillae Fulgentis) tree (Pterospermum heterophyllum Hance), or Hamamelidaceae Pterospermi Heterophylli (Semiliquidambar cathayensis Chang), or Moraceae Bai Guimu (Artocarpus hypargyraeaHance) and two color Artocarpus heterophyllus Lam (Red Hill prunus mume (sieb.) sieb.et zucc.s, Artocarpus styracifolius Pierre), or Lauraceae sassafrases (Sassafras tzumu Hemsl.), or Araliaceae tree ginseng (Dendropanax chevalieri (Vig) Men.) and change Ye Shucan (Dendropanax proteus Benth.).

Pharmaceutical composition of the present invention can be prepared according to conventional method of the prior art, extracts the active ingredient of each raw material of Chinese medicine, adds needed pharmaceutic adjuvant, makes the medicine of clinical any oral or exterior-applied formulation applicatory.Pharmaceutical composition of the present invention preferably is prepared by the method for the following stated.Drug combination preparation of the present invention or the preparation made by the inventive method can be the medicaments of acceptable any oral or exterior-applied formulation clinically, and its dosage form includes but not limited to capsule, soft capsule, tablet, pill, drop pill, granule, powder, patch, unguentum, gel, liquid preparation.

The pharmaceutical composition of expelling wind and removing dampness of the present invention, activating collaterals to relieve pain can be prepared by following method, and its step is as follows:

(1) getting by weight ratio whole raw material of Chinese medicine carries out decocting and obtains water extraction liquid; Precipitate with ethanol after the extract of perhaps fetching water is concentrated, obtain the precipitate with ethanol supernatant;

(2) make oral or external preparation after water extraction liquid or precipitate with ethanol supernatant concentration; Perhaps, water intaking extract or precipitate with ethanol supernatant concentration are made oral or external preparation by drying.

Pharmaceutical composition with expelling wind and removing dampness, relaxing muscles and tendons and activating QI and blood in the collateral, antalgic effect of the present invention can also be prepared by following method, and its step is as follows:

(1) get by weight ratio whole raw material of Chinese medicine and carry out alcohol extraction, alcohol extract concentrates to obtain the alcohol extraction concentrated solution; Perhaps, alcohol extraction concentrated solution water precipitating, the water precipitating supernatant concentration obtains the alcohol extracting-water precipitating concentrated solution;

(2) alcohol extraction concentrated solution or alcohol extracting-water precipitating concentrated solution are made to oral or external preparation; Perhaps, by alcohol extraction concentrated solution or alcohol extracting-water precipitating concentrated solution drying, make oral or external preparation.

The pharmaceutical composition of expelling wind and removing dampness of the present invention, activating collaterals to relieve pain can also be prepared by following method, and its step is as follows:

(1) get by weight ratio the flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis or 2 flavors or 3 flavors and extract volatile oil;

(2) medicinal residues merge alcohol extraction with the residue raw material of Chinese medicine, and alcohol extract is concentrated, adds volatile oil, makes oral or external preparation; Perhaps by the alcohol extract concentrate drying, after adding enclose or the volatile oil of enclose, do not make oral or external preparation.

The pharmaceutical composition of expelling wind and removing dampness of the present invention, activating collaterals to relieve pain can also be prepared by following method, gets by weight ratio whole raw material of Chinese medicine and carries out alcohol extraction, and alcohol extract is concentrated; The medicinal residues decocting, water extraction liquid is concentrated; Above-mentioned alcohol extraction concentrated solution, water extracting liquid are merged, make oral or external preparation; Perhaps, by alcohol extraction concentrated solution, water extracting liquid drying, make oral or external preparation.

(2) medicinal residues merge decocting with the residue raw material of Chinese medicine, and water extraction liquid concentrates to obtain water extracting liquid; Perhaps, the water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration obtains the water extract-alcohol precipitation concentrated solution;

(3) get water extracting liquid or water extract-alcohol precipitation concentrated solution, add volatile oil, make oral or external preparation; Perhaps, by water extracting liquid or water extract-alcohol precipitation concentrated solution drying, after adding enclose or the volatile oil of enclose, do not make oral or external preparation.

(2) medicinal residues merge alcohol extraction with the residue raw material of Chinese medicine, and alcohol extract is concentrated;

(3) medicinal residues decocting, water extraction liquid concentrates to obtain water extracting liquid; Perhaps, the water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration obtains the water extract-alcohol precipitation concentrated solution;

(4) water extracting liquid or water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution and volatile oil are merged, make oral or external preparation; Perhaps, by water extracting liquid or water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution drying, after adding enclose or the volatile oil of enclose, do not make oral or external preparation.

(1) get by weight ratio the flavor of 1 in Radix Schefflerae Arboricolae, Pterospermi Heterophylli or 2 flavor water extractions, water extraction liquid concentrates to obtain water extracting liquid; Perhaps water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration, obtain the water extract-alcohol precipitation concentrated solution;

(2) get by weight ratio the flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis or 2 flavors or 3 flavors and extract volatile oil, the medicinal residues alcohol extraction, alcohol extract is concentrated;

(3) water extracting liquid or water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution and volatile oil are merged, make oral or external preparation; Perhaps, by water extracting liquid or water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution drying, after adding enclose or the volatile oil of enclose, do not make oral or external preparation.

(1) get by weight ratio the flavor of 1 in Radix Schefflerae Arboricolae, Pterospermi Heterophylli or 2 flavor alcohol extractions, alcohol extract concentrates to obtain the alcohol extraction concentrated solution; Perhaps, alcohol extraction concentrated solution water precipitating, the water precipitating supernatant concentration obtains the alcohol extracting-water precipitating concentrated solution;

(2) get by weight ratio the flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis or 2 flavors or 3 flavors and extract volatile oil, the medicinal residues water extraction, water extraction liquid concentrates to obtain water extracting liquid; Perhaps water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration, obtain the water extract-alcohol precipitation concentrated solution;

(3) by above-mentioned alcohol extraction concentrated solution or alcohol extracting-water precipitating concentrated solution, water extracting liquid or water extract-alcohol precipitation concentrated solution and volatile oil merging, make oral or external preparation; Perhaps, by alcohol extraction concentrated solution or alcohol extracting-water precipitating concentrated solution, water extracting liquid or water extract-alcohol precipitation concentrated solution drying, after adding enclose or the volatile oil of enclose, do not make oral or external preparation.

(2) residue raw material of Chinese medicine alcohol extraction, alcohol extract is concentrated;

(3) water extracting liquid or water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution are merged, make oral or external preparation; Perhaps, by water extracting liquid or water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution drying, make oral or external preparation.

The pharmaceutical composition of expelling wind and removing dampness of the present invention, activating collaterals to relieve pain can also be prepared by following method, gets by weight ratio whole raw material of Chinese medicine and is pulverized, and makes oral or external preparation.

(1) get by weight ratio the flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis or 2 flavors or all or part of powder of beating of 3 flavor;

(2) residue raw material of Chinese medicine alcohol extraction together, mix with above-mentioned powder after alcohol extract is concentrated, makes oral or external preparation.

Expelling wind and removing dampness of the present invention, activating collaterals to relieve pain pharmaceutical composition can also be prepared by following method, its step is as follows:

(2) remain raw material of Chinese medicine water extraction together, the concentrated water extracting liquid that to obtain of water extraction liquid; Perhaps water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration, obtain the water extract-alcohol precipitation concentrated solution;

(3) water extracting liquid or water extract-alcohol precipitation concentrated solution and above-mentioned medicated powder are mixed, make oral or external preparation.Perhaps, water extracting liquid or water extract-alcohol precipitation concentrated solution and above-mentioned medicated powder are mixed, drying, make oral or external preparation.

(1) get by weight ratio the flavor of 1 in Radix Schefflerae Arboricolae, Pterospermi Heterophylli or all or part of powder of beating of 2 flavor;

(2) get by weight ratio the flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis or 2 flavors or 3 flavors and extract volatile oil; Medicinal residues merge alcohol extraction with the residue raw material of Chinese medicine, and alcohol extract is concentrated;

(3) alcohol extraction concentrated solution, above-mentioned medicated powder and volatile oil are mixed, make oral or external preparation; Perhaps, alcohol extraction concentrated solution, above-mentioned medicated powder are mixed, drying, after adding enclose or the volatile oil of enclose, do not make oral or external preparation.

(2) get by weight ratio the flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis or 2 flavors or 3 flavors and extract volatile oil; Medicinal residues merge water extraction with the residue raw material of Chinese medicine, and water extraction liquid concentrates to obtain water extracting liquid; Perhaps water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration, obtain the water extract-alcohol precipitation concentrated solution;

(3) water extracting liquid or water extract-alcohol precipitation concentrated solution and above-mentioned medicated powder and volatile oil are mixed, make oral or external preparation; Perhaps, water extracting liquid or water extract-alcohol precipitation concentrated solution and above-mentioned medicated powder are mixed, drying, after adding enclose or the volatile oil of enclose, do not make oral or external preparation.

In above-described preparation method technical scheme, the w/v of the water that water extraction or alcohol extraction add or ethanol and raw material, extraction time, extraction time etc. can be with reference to common process.

Below the drug action experiment that the inventor does:

One, Experimental agents

1, F1: prescription is, Rhizoma Alpiniae Officinarum, Flos Caryophylli, Radix Angelicae Sinensis, Radix Schefflerae Arboricolae, Pterospermi Heterophylli, and weight proportion is 1: 1: 1: 8: 8;

2, F2: prescription is, Rhizoma Alpiniae Officinarum, Flos Caryophylli, Radix Angelicae Sinensis, Radix Schefflerae Arboricolae, and weight proportion is 1: 1: 1: 16;

3, F3: prescription is, Rhizoma Alpiniae Officinarum, Flos Caryophylli, Radix Angelicae Sinensis, Pterospermi Heterophylli, and weight proportion is 1: 1: 1: 16;

Two, experimentation and result

1, F1, F2, the research of F3 arthritis

1.1 F1, F2, F3 are on the tentative arthritic impact of rabbit

Choose 36 of Chinchilla rabbit, body weight 1.8～2.2kg, male, adaptability is raised after one week and is formally started test, guarantees 20～25 ℃ of feeding environments in feeding process, and humidity is 40～70%.The same day was chosen 6 rabbit as blank group at random according to the rabbit body weight in modeling, all the other rabbit start sensitization, be between the rabbit back scapula and select 5 injection points, the emulsifying agent 0.2ml/ point that subcutaneous injection is formed by 3% egg protein solution and equal-volume incomplete Freund's adjuvant mixed configuration respectively.After sensitization, rabbit is normally raised, and after 28 days, in the rabbit right knee joint, only injects 3% egg protein solution 0.2ml/, carries out immune attack.

After immune attack 2 months, according to rabbit arthroncus degree, modeling group rabbit is divided into to 5 groups at random: i.e. model group (NS, 2.5ml/kg), F1 group (0.625g/kg, 2.5ml/kg), F2 group (0.625g/kg, 2.5ml/kg), F3 group (0.625g/kg, 2.5ml/kg) and ZHENGQINGFENGTONGNING group (0.02g/kg, 2.5ml/kg), divide into groups to start the same day administration.Administering mode is gastric infusion, once a day, and successive administration 3 months.

Each organized the rabbit successive administration after 3 months, got right knee joint and fixed with 10% formalin solution, did the routine pathology inspection.

The morphologic detection result is visible: the movable no abnormality seen of rabbit after sensitization, and after immune attack, second day is that visible attack joints has significant swelling, and the swelling degree peaks in about one week, and swelling degree is afterwards subdued gently gradually.The rabbit joint starts Secondary cases swelling after two months, with model group, compares, and each all can reduce rabbit joint Secondary cases swelling degree after organizing administration to a certain extent.

Histological examination: have no inflammatory cell in the normal group articular cavity and ooze out, synovial tissue is normal, and synovial cell's queueing discipline has no the lymphocyte nuclear monocyte infiltration, has no proliferation of fibrous tissue; In the model group articular cavity, inflammatory cell oozes out, and articular surface is coarse, erosion, articular cartilage and subchondral bone disorganization, and the cartilage surface pannus forms, and the synovial cell becomes multilamellar, take lymphocyte under synovial membrane as main inflammatory cell infiltration; Each administration group can reduce inflammatory cell oozes out, articular cartilage damage and synovial membrane inflammation.

Each organizes the pathological score data with mean ± standard deviation (x ± s) expression, analyzes the statistical software with SPSS13.0 forwindows, relatively uses one factor analysis of variance between group.Concrete pathological score statistical result is as following table 1.

The impact (x ± s) of table 1 F1～F3 side on rabbit experiment arthritis model joint pathology

With model group, compare ^*p<0.01

By upper table result, can be found out: the model group pathological score has been compared significant difference (P<0.01) with blank group, and the modeling success is described.F1, F2, F3 group and relatively all significantly reductions (P<0.01) of pathological score of model group, illustrate that these three prescriptions all have therapeutical effect preferably to this rabbit arthritis model.

1.2 F1, F2, the F3 impact on the rat test osteoarthritis

All rat experiments chamber adaptability is raised after three days grouping and modeling, the raising condition: relative humidity 40～70%, 20～24 ℃ of temperature, the feed of freely drinking water of starting.

Adaptability is chosen 10 rats at random as sham operated rats after raising and finishing, and all the other 50 adopt the Hulth method to set up rat knee joint OA model.By animal subject with after 10% chloral hydrate 0.3ml/100g intraperitoneal injection of anesthesia, lie on the back on the Rhizoma Atractylodis Macrocephalae platform, get the inboard longitudinal incision of right knee joint and be about 1cm, expose medial ligament and cut off, after open joint capsule, detect articular cavity cut off after without primary affection before and after ligamentaum cruciatum, the complete resection medial meniscus, the layer-by-layer suture wound, sterile dressing is fixed, postoperative antibiotic (ampicillin) two days, the prevention infection used.

After two weeks, all modeling rats are equally divided into to 5 groups at random: i.e. sham operated rats (NS, 20ml/kg), F1 group (1.625g/kg, 20ml/kg), F2 group (1.625g/kg, 20ml/kg), F3 group (1.625g/kg, 20ml/kg) and ZHENGQINGFENGTONGNING group (0.05g/kg, 2.5ml/kg), divide into groups to start administration the same day, once a day, be total to administration 30d.

After last administration 1h, put to death animal, dissect the right side knee joint, it is fixing that cartilage cuts the formalin solution of putting into 10%, for pathology detection.

Pathologic specimen routine pathology tissue slice, paraffin embedding, HE dyeing, microscopy.Articular cartilage tissue is learned Mankin ' the s method row pathological grading that adopts improvement, and grade scale is as following table 2, and appraisal result is for statistical analysis.

Table 2 articular cartilage tissue is learned Mankin ' grade form

Data mean with mean ± standard deviation (x ± s), analyze with SPSS 13.0 for windows statistical softwares, relatively use one factor analysis of variance between group.The results are shown in Table 3.

Micro-pathological observation: the arrangement of each layer of chondrocyte of sham operated rats and substrate be distributed with certain rule, cells of superficial layer becomes fusiformis, becomes horizontally.The intermediate cell circle, be dispersed in distribution, and the radiating layer chondrocyte becomes columnar arrangement.

There is significant depressions on model group cartilage top layer, the structure indistinguishable, and serious top layer disappears, the part district is the fine hair shape, and visible more crack is extended and deeply reached radiating layer downwards from the top layer cartilage, and in crack, visible sparse knot is formed group and loose netted connection, the fiber filling is arranged, damp heading line off.

The coarse injustice in ZHENGQINGFENGTONGNING group top layer, have crack, and a bunch collection cell increases, indivedual chondrocyte irregular arrangement, disorder.

F1 group bone 4 stratum boundary limits are unclear, but still can recognize, the chondrocyte rareness of top layer damaged part, and cell arrangement is owed rule; F2 and the pathological change of F3 group are slightly heavier than F1, must protective effect but also shown.

Exposure is the fine hair shape, and the cartilage columnar arrangement disappears.Medication group part specimen chondrocyte is the nido hypertrophy.

The impact (x ± s) of table 3 F1～F3 on rat experiment arthritis knuckle synovial membrane pathological score

With model group, compare ^*p<0.05, ^*p<0.01

Mark and analyze according to articular cartilage tissue Mankin ' s: model group and relatively sham operated rats score value increase obvious (P<0.01) show the model success.Three administration groups are compared score value with model group all have obvious decline (P<0.01), illustrates that F1, F2, tri-prescriptions of F3 can effectively improve the symptom of rat OA model.

2, F1, F2, the research of F3 analgesic activity.

2.1 F1, F2, F3 cause the impact of mice vola pain on hot plate.

Choose female ICR mice, body weight 18～22g, buy by oneself after affording 3d and tested.The mice sub-cage rearing, feeding environment temperature-controlled chamber temperature is 20～25 ℃, humidity is 40～70%, the ad lib water inlet.Get female mice, put into (55 ± 0.5) ℃ hot plate dolorimeter, using and lick metapedes as the pain reaction index, predict the front pain threshold of little raticide.Being less than 30s with pain threshold is greater than 5s and is considered as qualified mice.50 mices that pain threshold is qualified are divided into 5 groups at random, every group 10, be blank group of group (NS, 20ml/kg), F1 group (3.125g/kg, 20ml/kg), F2 group (3.125g/kg, 20ml/kg), F3 group (3.125g/kg, 20ml/kg) and positive control (tramadol hydrochloride) group (0.05g/kg, 20ml/kg).Administering mode is gastric infusion, and be administered once every day, altogether administration 3d.1h after the last administration, be placed on mice on the metallic plate of 55 ℃ of preheatings, constant temperature, and the mice of usining licks the incubation period of metapedes reaction as threshold of pain index, measures pain threshold.If in the mensuration process, 60s is interior without pain reaction, takes out immediately, and calculate with 60s.

Data mean with mean ± standard deviation (x ± s), analyze the statistical software with SPSS 13.0for windows, relatively use one factor analysis of variance between group.The results are shown in Table 4.

The impact (x ± s) of table 4 F1～F3 on the effect of hot plate method induced pain mice paroxysmal pain

With model group, compare: ^*p<0.01

Result shows, each administration group mice pain threshold and the significantly increases (P<0.01) of blank group.Results suggest F1, F2, tri-prescriptions of F3 all have the effect that stronger heat resistanceheat resistant plate causes mice vola pain.

2.2 F1, F2, F3 Dichlorodiphenyl Acetate cause the impact of mouse writhing pain.

50 of the ICR mices of body weight 18～22g, male and female half and half, minute cage adaptability is raised 3, freely drinks water, and raises with the laboratory animal mixed feed 20～23 ℃ of room temperatures, humidity 50～70%.Be divided at random 5 groups, 10 every group.Be blank group of (NS, 20ml/kg), F1 group (3.125g/kg, 20ml/kg), F2 group (3.125g/kg, 20ml/kg), F3 group (3.125g/kg, 20ml/kg) and positive control (rotundine) group (0.03g/kg, 20ml/kg).Administering mode is gastric infusion, and be administered once every day, altogether administration 3d.

Positive control (rotundine) group, dosage is the 0.03g/kg body weight, the blank group gives isometric normal saline.Administering mode is gastric infusion, and the administration volume is 20ml/kg, and be administered once every day, altogether administration 3d.

Last administration 1h respectively organizes mouse peritoneal and only injects 0.7% acetic acid normal saline solution 0.2ml/, use manual time-keeping simultaneously, the time of writhing response (being incubation period) and the interior writhing number of times occurred of 15min for the first time appear in every mice of observed and recorded, carry out statistical analysis.

Statistical result means with x ± s, adopts the one factor analysis of variance of SPSS 13.0 to be added up.The difference between each group relatively.The results are shown in Table 5.

Table 5 F1～F3 Dichlorodiphenyl Acetate causes the impact (x ± s) of mouse writhing reaction analgesic activity

With blank group, compare: ^*p<0.01

Result shows: medicine F1, F2 and F3 all can significantly reduce the mouse writhing number of times of acetic-acid induced, have compared significant difference (P<0.01) with model group.Illustrate that each group of F1, F2 and F3 all has the effect that stronger anti-acetic acid causes mouse writhing pain.

3, F1, F2, the research of F3 antiinflammatory action

3.1 F1, F2, F3 are on the granulomatous impact of rat button

Get 50 of SD rats, male and female half and half, body weight 130～170g.Sub-cage rearing, ad lib drinking-water, raise with the laboratory animal mixed feed, keeps 20～25 ℃ of room temperatures, humidity 40～70%.Formally start test after laboratory compatibility test 3d, rat is divided into to 5 groups at random, be respectively: model group (NS, 20ml/kg), F1 group (1.625g/kg, 20ml/kg), F2 group (1.625g/kg, 20ml/kg), F3 group (1.625g/kg, 20ml/kg) and positive control (dexamethasone) group (0.01g/kg, 20ml/kg).Each organizes rat ether light anaesthesia, wipes out the rat chesk hair, iodine disinfection, and the incision skin of chest, fill in respectively each, button from otch to two forelimb oxters, and experiment is consistent through gauze polishing assurance size and degree of roughness with button.Skin suture, intramuscular injection penicillin and streptomycin infection.Start gavage after postoperative 6d and give relative medicine, every day 1 time, continuous 10 days, after last administration 24h, rat is put to death in the cervical vertebra dislocation, peels off the button granulation tissue, dry 24h in 60 ℃ of baking ovens, electronic balance precise weighing (1/1000g), calculate granuloma weight, respectively organizes difference.

Data mean with mean ± standard deviation (x ± s), analyze the statistical software with SPSS13.0 for windows, relatively use one factor analysis of variance between group.The results are shown in Table 6.

Table 6 F1～F3 is on the granulomatous impact of rat button (x ± s)

^*p<0.05 is compared in expression with model group, ^*p<0.01 is compared in expression with model group

Result shows: with model group, compare, F1～F3 group can significantly reduce the granulomatous dry weight of rat button (P<0.01, P<0.05).Illustrate that each administration group of F1～F3 all has the granulomatous effect of the rat button of inhibition.

3.2 F1, F2, F3 Dichlorodiphenyl Acetate cause the impact that the mouse peritoneal capillary permeability increases.

Get 50 of ICR mices, male, body weight 18～22g.Mice is divided equally the cage raising, freely drinks water, and raises with the laboratory animal mixed feed, keeps 20～25 ℃ of room temperatures, humidity 40～70%.The mice adaptability is fed after three days and is started test.Be divided at random 5 groups, 10 every group.Be respectively: i.e. blank group of (NS, 20ml/kg), F1 group (3.125g/kg, 20ml/kg), F2 group (3.125g/kg, 20ml/kg), F3 group (3.125g/kg, 20ml/kg) and positive control (aspirin) group (0.2g/kg, 20ml/kg).Administering mode is gastric infusion, and be administered once every day, altogether administration 3d.

30min after the last administration, each organizes mouse mainline 0.5% azovan blue normal saline solution 0.1ml/10g, lumbar injection 0.6% acetic acid 0.2ml/ only immediately, put to death after 20min, cut off immediately skin of abdomen muscle, with 5ml normal saline washing abdominal cavity, the sucking-off cleaning mixture, 3000 leave heart 15min, get one times of supernatant dilution, measure each sample cell absorbance in the 590nm place.

Data mean with mean ± standard deviation (x ± s), analyze the statistical software with SPSS13.0 for windows, relatively use one factor analysis of variance between group.The results are shown in Table 7.

Table 7 F1～F3 Dichlorodiphenyl Acetate causes the impact (x ± s) that the mouse peritoneal capillary permeability increases

^*p<0.01 is compared in expression with model group

As can be seen from the above table, the absorbance of each administration group mouse peritoneal transudate is starkly lower than model control group (P<0.01).It can be said that tri-prescriptions of bright F1～F3 all can resist to a certain extent acetic acid and cause the effect that the mouse peritoneal permeability increases, there is antiphlogistic effects.

3.3 F1, F2, the F3 impact on mice auricle swelling

Get 50 of normal ICR mices, body weight 25～30g, male.Mus is divided equally the cage raising, freely drinks water, and raises with the laboratory animal mixed feed, keeps 20～25 ℃ of room temperatures, humidity 40～70%.The mice adaptability is fed after three days and is started test.Be divided at random 5 groups, 10 every group.Be respectively: blank group of (NS, 20ml/kg), F1 group (3.125g/kg, 20ml/kg), F2 group (3.125g/kg, 20ml/kg), F3 group (3.125g/kg, 20ml/kg) and positive control (aspirin) group (0.2g/kg, 20ml/kg).Administering mode is gastric infusion, and be administered once every day, altogether administration 3d.

Respectively organize mice after last administration 40min and be applied to two sides, mice left ear front and back with 2% Oleum Tiglii 0.05ml respectively, put to death mice after causing scorching 4h, cut left and right two ears along the auricle baseline, take off round auricle at same position respectively with card punch (diameter 9mm), scales/electronic balance weighing, using the difference of mice left and right auricle weight as auricle swelling degree, and calculate the swelling percentage rate.

Data mean with mean ± standard deviation (x ± s), analyze with SPSS 13.0 for windows statistical softwares, relatively use one factor analysis of variance between group.The results are shown in Table 8.

The impact ((x ± s) of table 8 F1～F3 on Oleum Tiglii induced mice ear swelling

With blank group, compare, ^*p<0.05, ^*p<0.01

As can be seen from the above table: after administration, F1, F2, F3 group mice auricle swelling degree are compared the reduction (P<0.05, P<0.01) had in various degree with model group.Can show that thus tri-prescriptions of F1～F3 have shown the swollen inflammatory effect of certain anti-Mus ear.

The inventor, by going deep into systematic study, has filtered out the significant Chinese medicine formula of antalgic and inflammation relieving effect.Compared with prior art, pharmaceutical composition of the present invention has good safety, remarkable antalgic and inflammation relieving, obviously improve the various symptoms that Chinese medical discrimination belongs to the arthralgia due to wind-cold-dampness disease patient, be applicable to the treatment of rheumatic arthritis, rheumatoid arthritis, numb limbs and tense tendons, back cold type of pain, scapulohumeral periarthritis, neck-shoulder pain, lumbago and leg pain, sciatica, trigeminal neuralgia, hyperosteogeny, prolapse of lumbar intervertebral disc, postpartum apoplexy etc.

The specific embodiment

Below further describe concrete technical scheme of the present invention, so that those skilled in the art understands the present invention further, and do not form the restriction to its right.

Embodiment 1.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 0.1; Flos Caryophylli 0.1; Radix Angelicae Sinensis 0.1; Radix Schefflerae Arboricolae 0.6; Pterospermi Heterophylli 0.8.Technology extracts the active ingredient of each raw material routinely, adds conventional pharmaceutic adjuvant, makes oral or external preparation.

Embodiment 2.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 0.1; Flos Caryophylli 0.1; Radix Angelicae Sinensis 0.1; Radix Schefflerae Arboricolae 30; Pterospermi Heterophylli 30.Its preparation method is to get by weight ratio whole raw material of Chinese medicine to be pulverized, and makes powder or honeyed pill.

Embodiment 3.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 1; Flos Caryophylli 1; Radix Angelicae Sinensis 1; Radix Schefflerae Arboricolae 10; Pterospermi Heterophylli 10.Its preparation method is: get by weight ratio whole raw material of Chinese medicine and carry out decocting and obtain water extraction liquid, after water extraction liquid is concentrated, make oral or external preparation.

Embodiment 4.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 2; Flos Caryophylli 1; Radix Angelicae Sinensis 3; Radix Schefflerae Arboricolae 20.Its preparation method is: gets by weight ratio whole raw material of Chinese medicine and carries out decocting and obtain water extraction liquid, and precipitate with ethanol after water extraction liquid is concentrated, the precipitate with ethanol supernatant concentration is made capsule by drying.

Embodiment 5.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 2; Flos Caryophylli 2; Radix Angelicae Sinensis 2; Pterospermi Heterophylli 18.Its preparation method is: get by weight ratio whole raw material of Chinese medicine and carry out alcohol extraction, after alcohol extract is concentrated, make oral or external preparation, or concentratedly by drying, make external preparation.

Embodiment 6.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 4; Flos Caryophylli 3; Radix Angelicae Sinensis 6; Pterospermi Heterophylli 12.Its preparation method is: gets by weight ratio whole raw material of Chinese medicine and carries out alcohol extraction, and water precipitating after alcohol extract is concentrated, the water precipitating supernatant concentration, make soft capsule, or drying is made capsule.

Embodiment 7.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 6; Flos Caryophylli 6; Radix Angelicae Sinensis 6; Radix Schefflerae Arboricolae 6; Pterospermi Heterophylli 6.Its preparation method is: get by weight ratio Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis 3 flavor extraction volatile oil; Medicinal residues merge alcohol extraction with the residue raw material of Chinese medicine, and alcohol extract is concentrated, adds volatile oil, makes oral soft capsule.

Embodiment 8.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 1.5; Flos Caryophylli 1.5; Radix Angelicae Sinensis 1.5; Radix Schefflerae Arboricolae 15.Its preparation method is: get by weight ratio Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis 3 flavor extraction volatile oil; Medicinal residues merge alcohol extraction with the residue raw material of Chinese medicine, the alcohol extract concentrate drying, and after adding enclose or the volatile oil of enclose, do not make capsule.

Embodiment 9.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 2; Flos Caryophylli 2; Radix Angelicae Sinensis 2; Radix Schefflerae Arboricolae 2; Pterospermi Heterophylli 2.Its preparation method is: get by weight ratio whole raw material of Chinese medicine and carry out alcohol extraction, alcohol extract is concentrated; The medicinal residues decocting, water extraction liquid is concentrated; Above-mentioned alcohol extraction concentrated solution, water extracting liquid are merged, make externally applied ointment.

Embodiment 10.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 1; Flos Caryophylli 1; Radix Angelicae Sinensis 1; Radix Schefflerae Arboricolae 2; Pterospermi Heterophylli 8.Its preparation method is: get by weight ratio Flos Caryophylli, Radix Angelicae Sinensis 2 flavor extraction volatile oil; Medicinal residues merge decocting with the residue raw material of Chinese medicine, and by water extraction liquid concentrate drying, after adding enclose or the volatile oil of enclose, do not make oral or external preparation.

Embodiment 11.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 2; Flos Caryophylli 1; Radix Angelicae Sinensis 3; Radix Schefflerae Arboricolae 8; Pterospermi Heterophylli 2.Its preparation method is: get by weight ratio Flos Caryophylli, Rhizoma Alpiniae Officinarum 2 flavor extraction volatile oil; Medicinal residues merge water extraction with the residue raw material of Chinese medicine, precipitate with ethanol after water extraction liquid is concentrated, and precipitate with ethanol supernatant contracting liquid, add volatile oil, makes oral or external preparation;

Embodiment 12.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 2; Flos Caryophylli 1; Radix Angelicae Sinensis 3; Pterospermi Heterophylli 10.Its preparation method is: get by weight ratio Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis 3 flavor extraction volatile oil; Medicinal residues merge alcohol extraction with the residue raw material of Chinese medicine, and alcohol extract is concentrated; The medicinal residues decocting, water extraction liquid is concentrated; By water extracting liquid, alcohol extraction concentrated solution drying, after adding enclose or the volatile oil of enclose, do not make oral or external preparation.

Embodiment 13.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 2; Flos Caryophylli 1; Radix Angelicae Sinensis 3; Pterospermi Heterophylli 10.Its preparation method is: get by weight ratio the Pterospermi Heterophylli water extraction, water extraction liquid is concentrated, precipitate with ethanol, precipitate with ethanol supernatant concentration; Get by weight ratio Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis 3 flavor extraction volatile oil, the medicinal residues alcohol extraction, alcohol extract is concentrated; Water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution and volatile oil are merged, make oral or external preparation; Perhaps, by water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution drying, after adding enclose or the volatile oil of enclose, do not make oral or external preparation.

Embodiment 14.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 3; Flos Caryophylli 1; Radix Angelicae Sinensis 6; Radix Schefflerae Arboricolae 8; Pterospermi Heterophylli 12.Its preparation method is: get Radix Schefflerae Arboricolae, Pterospermi Heterophylli 2 flavor water extractions, water extraction liquid is concentrated; Get by weight ratio Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis 3 flavor extraction volatile oil, the medicinal residues alcohol extraction, alcohol extract is concentrated; Water extracting liquid, alcohol extraction concentrated solution and volatile oil are merged, make oral or external preparation; Perhaps, by water extracting liquid, alcohol extraction concentrated solution drying, after adding enclose or the volatile oil of enclose, do not make oral or external preparation.

Embodiment 15.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 6; Flos Caryophylli 3; Radix Angelicae Sinensis 6; Radix Schefflerae Arboricolae 15.Its preparation method is: get by weight ratio the Radix Schefflerae Arboricolae alcohol extraction, alcohol extract is concentrated; Get Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis 3 flavor extraction volatile oil, the medicinal residues water extraction, water extraction liquid is concentrated; Water extracting liquid, alcohol extraction concentrated solution and volatile oil are merged, make oral or external preparation; Perhaps, by water extracting liquid, alcohol extraction concentrated solution drying, after adding enclose or the volatile oil of enclose, do not make oral or external preparation.

Embodiment 16.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 2; Flos Caryophylli 1; Radix Angelicae Sinensis 8; Radix Schefflerae Arboricolae 9.Its preparation method is: get by weight ratio the Radix Schefflerae Arboricolae alcohol extraction, alcohol extract concentrates water precipitating, the water precipitating supernatant concentration; Get Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis 3 flavor extraction volatile oil, the medicinal residues water extraction, water extraction liquid is concentrated; Water extracting liquid, alcohol extracting-water precipitating concentrated solution and volatile oil are merged, make oral or external preparation; Perhaps, by water extracting liquid, alcohol extracting-water precipitating concentrated solution drying, after adding enclose or the volatile oil of enclose, do not make oral or external preparation.

Embodiment 17.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 3; Flos Caryophylli 1; Radix Angelicae Sinensis 1; Radix Schefflerae Arboricolae 6.Its preparation method is: get by weight ratio the Radix Schefflerae Arboricolae water extraction, water extraction liquid is concentrated; The alcohol extraction of residue raw material of Chinese medicine, alcohol extract is concentrated; Water extracting liquid, alcohol extraction concentrated solution are merged, make oral or external preparation; Perhaps, by water extracting liquid, alcohol extraction concentrated solution drying, make oral or external preparation.

Embodiment 18.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 0.9; Flos Caryophylli 0.9; Radix Angelicae Sinensis 0.9; Radix Schefflerae Arboricolae 3; Pterospermi Heterophylli 3.Its preparation method is: get by weight ratio whole raw material of Chinese medicine and pulverized, make oral or external preparation.

Embodiment 19.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 3; Flos Caryophylli 3; Radix Angelicae Sinensis 3; Radix Schefflerae Arboricolae 6; Pterospermi Heterophylli 6.Its preparation method is: get by weight ratio Flos Caryophylli, Rhizoma Alpiniae Officinarum, the whole powder of beating of Radix Angelicae Sinensis 3 flavors; The residue raw material of Chinese medicine merges alcohol extraction, after alcohol extract is concentrated, with above-mentioned powder, mixes, and makes oral or external preparation.

Embodiment 20.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 3; Flos Caryophylli 3; Radix Angelicae Sinensis 3; Radix Schefflerae Arboricolae 2; Pterospermi Heterophylli 2.Its preparation method is: get by weight ratio Flos Caryophylli, the whole powder of beating of Rhizoma Alpiniae Officinarum 2 flavors; The residue raw material of Chinese medicine is water extraction together, and water extraction liquid is concentrated; Water extracting liquid and above-mentioned medicated powder are mixed, make oral or external preparation.Perhaps, water extracting liquid and above-mentioned medicated powder are mixed, drying, make oral or external preparation.

Embodiment 21.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 1.5; Flos Caryophylli 1.5; Radix Angelicae Sinensis 1.5; Pterospermi Heterophylli 5.Its preparation method is: get by weight ratio Pterospermi Heterophylli and all beat powder; Get by weight ratio Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis 3 flavor extraction volatile oil, the medicinal residues alcohol extraction; Alcohol extraction concentrated solution, above-mentioned medicated powder and volatile oil are mixed, make oral or external preparation; Perhaps, alcohol extraction concentrated solution, above-mentioned medicated powder are mixed, drying, after adding enclose or the volatile oil of enclose, do not make oral or external preparation.

Embodiment 22.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 15; Flos Caryophylli 15; Radix Angelicae Sinensis 15; Pterospermi Heterophylli 30.Its preparation method is: by weight ratio half got Pterospermi Heterophylli and beaten powder; Get by weight ratio Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis 3 flavor extraction volatile oil; Medicinal residues merge water extraction with the residue raw material of Chinese medicine, and water extraction liquid concentrates precipitate with ethanol, precipitate with ethanol supernatant concentration; Water extract-alcohol precipitation concentrated solution and above-mentioned medicated powder and volatile oil are mixed, make oral or external preparation; Perhaps, water extract-alcohol precipitation concentrated solution and above-mentioned medicated powder are mixed, drying, after adding enclose or the volatile oil of enclose, do not make oral or external preparation.

Embodiment 23.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 15; Flos Caryophylli 15; Radix Angelicae Sinensis 15; Radix Schefflerae Arboricolae 30; Pterospermi Heterophylli 30.Its preparation method is: get by weight ratio whole raw material of Chinese medicine and carry out decocting and obtain water extraction liquid; Make oral or external preparation after water extraction liquid is concentrated.

Embodiment 24.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 0.5; Flos Caryophylli 0.5; Radix Angelicae Sinensis 0.5; Radix Schefflerae Arboricolae 0.5; Pterospermi Heterophylli 0.5.Its preparation method is: get by weight ratio whole raw material of Chinese medicine and carry out decocting and obtain water extraction liquid, precipitate with ethanol after the water intaking extract is concentrated, obtain the precipitate with ethanol supernatant; Make oral or external preparation after the precipitate with ethanol supernatant concentration.

Embodiment 25.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 0.5; Flos Caryophylli 0.5; Radix Angelicae Sinensis 0.5; Radix Schefflerae Arboricolae 0.5; Pterospermi Heterophylli 0.5.Its preparation method is: get by weight ratio whole raw material of Chinese medicine and carry out decocting and obtain water extraction liquid; The water intaking extract is concentrated makes oral or external preparation by drying.

Embodiment 26.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 0.1; Flos Caryophylli 0.1; Radix Angelicae Sinensis 0.1; Its preparation method is: get by weight ratio whole raw material of Chinese medicine and carry out decocting and obtain water extraction liquid; The water intaking extract is concentrated makes oral or external preparation by drying.

Embodiment 27.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 6; Flos Caryophylli 3; Radix Angelicae Sinensis 9; Radix Schefflerae Arboricolae 20; Pterospermi Heterophylli 20.Its preparation method is: get by weight ratio whole raw material of Chinese medicine and carry out decocting and obtain water extraction liquid, precipitate with ethanol after the water intaking extract is concentrated, obtain the precipitate with ethanol supernatant; The precipitate with ethanol supernatant concentration is made oral or external preparation by drying.

Embodiment 28.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 6; Flos Caryophylli 3; Radix Angelicae Sinensis 9; Radix Schefflerae Arboricolae 0.5; Pterospermi Heterophylli 0.5.Its preparation method is: get by weight ratio whole raw material of Chinese medicine and carry out alcohol extraction, alcohol extract concentrates to obtain the alcohol extraction concentrated solution; Perhaps, alcohol extraction concentrated solution water precipitating, the water precipitating supernatant concentration obtains the alcohol extracting-water precipitating concentrated solution; Alcohol extraction concentrated solution or alcohol extracting-water precipitating concentrated solution are made to oral or external preparation; Perhaps, by alcohol extraction concentrated solution or alcohol extracting-water precipitating concentrated solution drying, make oral or external preparation.

Embodiment 29.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 0.5; Flos Caryophylli 0.5; Radix Angelicae Sinensis 0.5; Radix Schefflerae Arboricolae 20; Pterospermi Heterophylli 20.Its preparation method is: get by weight ratio the flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis or 2 flavors or 3 flavors and extract volatile oil; Medicinal residues merge alcohol extraction with the residue raw material of Chinese medicine, and alcohol extract is concentrated, adds volatile oil, makes oral or external preparation; Perhaps by the alcohol extract concentrate drying, after adding enclose or the volatile oil of enclose, do not make oral or external preparation.

Embodiment 30.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 0.1; Flos Caryophylli 0.1; Radix Angelicae Sinensis 0.1; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 30; Its preparation method is: get by weight ratio whole raw material of Chinese medicine and carry out alcohol extraction, alcohol extract is concentrated; The medicinal residues decocting, water extraction liquid is concentrated; Above-mentioned alcohol extraction concentrated solution, water extracting liquid are merged, make oral or external preparation; Perhaps, by alcohol extraction concentrated solution, water extracting liquid drying, make oral or external preparation.

Embodiment 31.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 15; Flos Caryophylli 15; Radix Angelicae Sinensis 15; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 0.5.Its preparation method is: get by weight ratio the flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis or 2 flavors or 3 flavors and extract volatile oil; Medicinal residues merge decocting with the residue raw material of Chinese medicine, and water extraction liquid concentrates to obtain water extracting liquid; Perhaps, the water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration obtains the water extract-alcohol precipitation concentrated solution; Get water extracting liquid or water extract-alcohol precipitation concentrated solution, add volatile oil, make oral or external preparation; Perhaps, by water extracting liquid or water extract-alcohol precipitation concentrated solution drying, after adding enclose or the volatile oil of enclose, do not make oral or external preparation.

Embodiment 32.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 0.5; Flos Caryophylli 0.5; Radix Angelicae Sinensis 0.5; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 20.Its preparation method is:

Embodiment 33.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 6; Flos Caryophylli 3; Radix Angelicae Sinensis 9; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 0.5.Its preparation method is:

Embodiment 34.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 0.5; Flos Caryophylli 0.5; Radix Angelicae Sinensis 0.5; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 0.5.Its preparation method is:

Embodiment 35.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 6; Flos Caryophylli 3; Radix Angelicae Sinensis 9; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 20.Its preparation method is:

Embodiment 36.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 4; Flos Caryophylli 4; Radix Angelicae Sinensis 10; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 15.Its preparation method is: get by weight ratio whole raw material of Chinese medicine and pulverized, make oral or external preparation.

Embodiment 37.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 15; Flos Caryophylli 15; Radix Angelicae Sinensis 15; Its preparation method is: get by weight ratio whole raw material of Chinese medicine and pulverized, make oral or external preparation.

Embodiment 38.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 4; Flos Caryophylli 4; Radix Angelicae Sinensis 10; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 15.Its preparation method is:

Embodiment 39.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 10; Flos Caryophylli 8; Radix Angelicae Sinensis 15; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 25.Its preparation method is:

Embodiment 40.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 2; Flos Caryophylli 12; Radix Angelicae Sinensis 10; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 18.Its preparation method is:

(1) get by weight ratio all or part of powder of beating of Radix Schefflerae Arboricolae or Pterospermi Heterophylli;

Embodiment 41.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by the raw material of Chinese medicine of following weight proportion, Rhizoma Alpiniae Officinarum 0.5; Flos Caryophylli 1; Radix Angelicae Sinensis 0.8; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 15.Its preparation method is:

Claims

1. the pharmaceutical composition of an expelling wind and removing dampness, activating collaterals to relieve pain, is characterized in that, it is to be made by the raw material of Chinese medicine of following weight proportion:

Radix Schefflerae Arboricolae 0.1～30 and Pterospermi Heterophylli 0.1～30.

2. the preparation method of a pharmaceutical composition as claimed in claim 1, is characterized in that, its step is as follows:

3. the preparation method of a pharmaceutical composition as claimed in claim 1, is characterized in that,

4. the preparation method of a pharmaceutical composition as claimed in claim 1, is characterized in that, its step is as follows:

5. the preparation method of a pharmaceutical composition as claimed in claim 1, is characterized in that, gets by weight ratio whole raw material of Chinese medicine and carry out alcohol extraction, and alcohol extract is concentrated; The medicinal residues decocting, water extraction liquid is concentrated; Above-mentioned alcohol extraction concentrated solution, water extracting liquid are merged, make oral or external preparation; Perhaps, by alcohol extraction concentrated solution, water extracting liquid drying, make oral or external preparation.

6. the preparation method of a pharmaceutical composition as claimed in claim 1, is characterized in that, its step is as follows:

7. the preparation method of a pharmaceutical composition as claimed in claim 1, is characterized in that, its step is as follows:

8. the preparation method of a pharmaceutical composition as claimed in claim 1, is characterized in that, its step is as follows,

9. the preparation method of a pharmaceutical composition as claimed in claim 1, is characterized in that, gets by weight ratio whole raw material of Chinese medicine and pulverized, and makes oral or external preparation.

10. the preparation method of a pharmaceutical composition as claimed in claim 1, is characterized in that, its step is as follows:

11. the preparation method of a pharmaceutical composition as claimed in claim 1, is characterized in that, its step is as follows:

(3) water extracting liquid or water extract-alcohol precipitation concentrated solution and above-mentioned medicated powder are mixed, make oral or external preparation; Perhaps, water extracting liquid or water extract-alcohol precipitation concentrated solution and above-mentioned medicated powder are mixed, drying, make oral or external preparation.

12. the preparation method of a pharmaceutical composition as claimed in claim 1, is characterized in that, its step is as follows,

13. the preparation method of a pharmaceutical composition as claimed in claim 1, is characterized in that, its step is as follows,

A 14. pharmaceutical composition as claimed in claim 1 or as claim 2～13 drug combination preparation that preparation method makes as described in any one, it is characterized in that, described preparation is capsule, tablet, pill, drop pill, granule, powder, patch, unguentum, gel or liquid preparation.

15. pharmaceutical composition as claimed in claim 1 or preparation as claimed in claim 14 are as the application in the medicine of preparation treatment rheumatic arthritis, rheumatoid arthritis, numb limbs and tense tendons, back cold type of pain, scapulohumeral periarthritis, neck-shoulder pain, lumbago and leg pain, sciatica, trigeminal neuralgia, hyperosteogeny, prolapse of lumbar intervertebral disc or postpartum apoplexy.