CN105853481B - The application of Hu Ci Aralia wood extract or the Chinese medicine composition containing Hu Ci Aralia wood extract - Google Patents
The application of Hu Ci Aralia wood extract or the Chinese medicine composition containing Hu Ci Aralia wood extract Download PDFInfo
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Abstract
Application of the Chinese medicine composition the invention discloses tiger thorn Aralia wood extract or containing tiger thorn Aralia wood extract in the product that preparation has anti-pig virus diarrhoea effect; tiger thorn Aralia wood extract can safely and effectively prevent and/or treat Porcine epidemic diarrhea virus using the sufficient tiger thorn Aralia wood in source as raw material.Hu Ci Aralia wood extract of the present invention or Chinese medicine composition containing Hu Ci Aralia wood extract are compared with existing product, the anti-pig virus diarrhoea effect of Chinese medical extract Hu Ci Aralia wood extract of the present invention or the Chinese medicine composition containing Hu Ci Aralia wood extract is better than the effect of existing product, therefore in the manufacturing process of its additive for being applicable to anti-pig virus diarrhoea, drug and feed.
Description
Technical field
The invention belongs to pig feed additive technical fields, and in particular to a kind of Hu Ci Aralia wood extract contains Hu Ci Aralia
Application of the Chinese medicine composition of the wooden extract in the product that preparation has anti-pig virus diarrhoea effect.
Background technique
Pig is in consequence in the animal husbandry in China, and pork is then the emphasis of China's animal husbandry product, because
This, the health status of pig has a direct influence for the food safety in China, the disease of pig especially Disease it is pre-
What anti-and treatment became more and more important, the generation and sprawling for how preventing and treating pig disease are current things urgently to be solved.With
The continuous development of society, the disease problems of China pig become more and more prominent at present, the various frequent generations about pig disease,
Have become an important factor for restricting the development of China's pig breeding industry.In recent years, the pig illness rate of individual farmer breed is higher and higher, this
The benefit damage for directly resulting in peasant is serious.The disease (more than common disease) of pig is prevented and treated, and takes pig disease
Complete, complete precautionary measures, avoid transmission and diffusion, protect the economic interests of pig farmer, tool has very important significance.
Recent two decades, in domestic Prevention of Common Occurrence Porcine Disease, viral infectious is there are about 16 kinds, including swine fever, blue otopathy, circovirus
Disease, aftosa, pseudoabies, encephalitis B, parvovirus, transmissible gastroenteritis etc., and kainogenesis infectious disease is mostly
Viral disease.In recent years, with the development of pig breeding industry, pig virus diarrhoea have become endanger pig most serious disease it
One.Pig virus diarrhoea is increasingly severe, brings huge economic loss to pig farmer (field).The disease causes a large amount of piglet abdomen
Rush down and cause piglet high mortality, and antibiotic directly results in a lot of raisers (field) in vain and look at all to viral have loose bowels
The method having loose bowels less than treatment pig virus.Virosis has infectiousness big, and prevalence is wide, the death rate is high, caused economic damage
Lose huge feature.Since the aggregate measures prevented epidemic to swine disease implement ineffective, virus subculture and intersection repeatedly in inhibiting environment
The long-term of infection, mixed infection and clinic is increased with malicious and atypical case, brings difficulty to diagnosing and treating.In addition,
Effective drug is still lacked to the treatment of viral disease at present.Because virus replicative cycle include adsorb, penetrate, shelling,
The stages such as biosynthesis and assembly release, it is known that existing antiviral agent, preferably at most a certain medicine is to a certain virus replication mistake
Certain born of the same parents of journey, but the antiviral agent truly of energy inactivation of viruses is worldwide not yet succeeded in developing.
Immunization techniques and drug control methods are mainly used virosis at present, though immunization techniques prevention and treatment swine flu can
There is provided certain protection, and presently, there are also many with the Vaccines classes studied, different vaccines have it corresponding excellent
Gesture, but there is also many shortcomings.Drug control aspect, is on the one hand that Western medicine produces drug resistance after continued use, separately
On the one hand be that Western medicine using food-safe cannot be neglected influences, thus in veterinary clinic using upper with certain office
It is sex-limited.Therefore it compares therewith, natural Chinese medicinal herb is then because its toxic side effect is small, cheap, abundance can be sent out in terms of a variety of
It waves antiviral effect and there is incomparable advantage.
Hu Ci Aralia wood: Araliaceae dissipates fogfruit Damnacamthus indicus wood Araliaarmata (Wall.) Seem, alias Guangdong wood, hundred
Bird does not fall, wild in some provinces and regions of south China.Hu Ci Aralia wood bitter and puckery flavor, it is cool in nature, mainly it is used as medicine with root skin and root, it is main
Ingredient is oleanolic acid, is usually used in treating rheumatic arthralgia, arthritis, traumatic injury, hepatitis, cirrhosis, nephritic dropsy, prostate
Inflammation, stomach trouble, boil etc..Have not yet to see the report in relation to Hu Ci Aralia wood in anti-virus aspect.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of Hu Ci Aralia wood extract or containing Hu Ci Aralia wood extract
Application of the Chinese medicine composition in the product that preparation has anti-pig virus diarrhoea effect, tiger thorn Aralia wood extract is with source abundance
Hu Ci Aralia wood be raw material, can safely and effectively prevent and/or treat Porcine epidemic diarrhea virus.
To achieve the goals above, the invention provides the following technical scheme: Hu Ci Aralia wood extract or contain Hu Ci Aralia wood
Application of the Chinese medicine composition of extract in the product that preparation has anti-pig virus diarrhoea effect；Described contains Hu Ci Aralia
The Chinese medicine composition of the wooden extract is Hu Ci Aralia wood extract, Cacumen Securinegae Suffruticosae extract and Herba Abri extract, the Hu Ci Aralia wood
The proportion by weight of extract, Cacumen Securinegae Suffruticosae extract and Herba Abri extract is 10~20:1~3:1~3.
As a preferred technical solution of the invention, the Hu Ci Aralia wood extract, which is prepared by the following method, to be obtained
: it chooses Hu Ci Aralia wood, ethyl alcohol is added after crushing, is filtered after heating and refluxing extraction, after filtrate is concentrated to obtain the final product.
In above-mentioned preferred technical solution: the proportion by weight of the Hu Ci Aralia wood and ethyl alcohol is preferably 1:3~10,
The volumn concentration of middle ethyl alcohol is preferably 30~95%, and most preferably 60%, each extraction time is preferably 30~60min, is mentioned
Taking number is 1~3 time, most preferably 2 times, concentrate the filtrate to the 1/5~2/5 of filtrate original volume to obtain the final product, most preferably original volume
1/3。
Further, Hu Ci Aralia wood extract of the present invention is prepared by the following method acquisition: described contains tiger
The Chinese medicine composition of thorn Aralia wood extract is prepared by the following method acquisition: after taking tiger thorn Aralia wood to go disleaf, staying root and skin powder
It is broken, it is filtered after obtained material ethyl alcohol heating and refluxing extraction being crushed, is Chinese medical extract after filtrate concentration.
As the preferred technical solution of another kind of the invention, the Chinese medicine composition containing Hu Ci Aralia wood extract
It is prepared by the following method acquisition: choosing Hu Ci Aralia wood, Common Leafflower Herb and Canton love-pea vine, be separately added into ethyl alcohol after crushing, be heated to reflux
It is filtered after extraction, up to Hu Ci Aralia wood extract, Cacumen Securinegae Suffruticosae extract and Herba Abri extract after filtrate is concentrated, by Hu Ci Aralia
The wooden extract, Cacumen Securinegae Suffruticosae extract and Herba Abri extract are mixed by above-mentioned proportion by weight and are extracted to get containing Hu Ci Aralia wood
The Chinese medicine composition of object.
In the preferred technical solution: the proportion by weight of the Hu Ci Aralia wood, Common Leafflower Herb and Canton love-pea vine and ethyl alcohol
Respectively 1:3~10, the volumn concentration of ethyl alcohol are 30~95%, and each extraction time is 30~60min, and extraction time is
1~3 time, concentrate the filtrate to the 1/5~2/5 of filtrate original volume to obtain the final product.
Tiger thorn Aralia wood, Common Leafflower Herb and Canton love-pea vine can also first be mixed and ethyl alcohol extraction is used together again.
Product of the present invention is preferably additive, drug or feed, and the feed preferably includes the extraction of Hu Ci Aralia wood
Object and pig basal feed, wherein the proportion by weight of Hu Ci Aralia wood extract and pig basal feed is 0.8~1.2:20, more preferably
1:20.
It is raw material that the present invention, which selects Hu Ci Aralia wood, and prepared by ethyl alcohol formulation, substantially increase the content and drug effect of medicine, utilizes
It is prepared into a kind of Chinese medical extract novel, with antivirus action, and Chinese medical extract bulk pharmaceutical chemicals of the present invention are not only
Source is sufficient, and antiviral effect is better than existing product, can be widely applied to antiviral agent especially beasts antiviral agent
In preparation with the additive of feed, use is simple and effective, and it is stable, at low cost that invention animal medicinal composition treats simple process, quality
It is honest and clean, toxic side effect is small, can adjust macrophage function, liver and gallbladder protection improves pig immunity, inhibits white thin in affected pig blood
The decline of born of the same parents' sum has remarkable result to the treatment of pig Porcine epidemic diarrhea virus, has huge market potential.
Mainly there are vaccine control, antiviral chemical synthetic drug and exogenous cells to the control method of virosis at present
Factor in treatment etc..Vaccine control is most common method, but viral species are more, and serotype is more, and influenza virus variability is high, this is
The application of vaccine brings certain difficulty；Antiviral Western medicine negligible amounts, clinical effectiveness is undesirable, and never has for many years
New antiviral synthetic drug generates, and the effect of some drugs is still in conceptual phase, and some drugs do not allow still to face in animal doctor
It is applied on bed；The energy suppressing virus replication such as medical exogenous interferon, interleukin 2, improves Immune Function,
Treatment viral disease has good prospects, but somewhat expensive, and remains in basic research and clinical experimental stage at present.And Chinese medicine
Medicine clinical prevention toxic side effect is small, and medicine source is abundant, cheap, is adjustable whole immune function, and suppressing virus replication prevents disease
Malicious cytopathogenic effect improves clinical symptoms, shows unique advantage in terms for the treatment of disease of viral infection.
To sum up, though immunization techniques prevention and treatment swine flu is capable of providing certain protection, and presently, there are with studying
Vaccines classes it is also very much, different vaccines have its corresponding advantage, but there is also many shortcomings.Drug control aspect,
Certain artificial chemistry synthetic drugs have proven to the antivirus action for having certain, but have certain office using upper in veterinary clinic
It is sex-limited；And cell factor such as interferon, interleukin etc. truly has the effect for hindering virus replication, but its not only somewhat expensive, and mesh
It is preceding still in basic research and experimental stage；It compares therewith, natural Chinese medicinal herb is then because its toxic side effect is small, cheap, source
It is abundant to play antiviral effect in terms of a variety of and there is incomparable advantage.
The present invention has the advantage that the Hu Ci Aralia wood extract in the present invention or the Chinese medicine containing Hu Ci Aralia wood extract
Composition compared with existing product, both use minimal lethal dose under the premise of, Hu Ci Aralia wood extract of the present invention or
The anti-pig virus diarrhoea effect of Chinese medicine composition containing Hu Ci Aralia wood extract is better than the effect of existing product, therefore its energy
It is enough applied in the manufacturing process of the additive of anti-pig virus diarrhoea, drug and feed, moreover, the survival rate in pig is tested
In, Hu Ci Aralia wood extract of the present invention or the Chinese medicine composition containing Hu Ci Aralia wood extract can be improved the cultivation of these beasts and move
The survival rate of object, is better than existing product.
Specific embodiment
Chinese medicine composition the invention discloses Hu Ci Aralia wood extract or containing Hu Ci Aralia wood extract has in preparation
Application in the product of anti-pig virus diarrhoea effect, those skilled in the art can use for reference present disclosure, be suitably modified technique
Parameter is realized.In particular, it should be pointed out that all similar substitutions and modifications are apparent for a person skilled in the art
, they are considered as being included in the present invention.Product of the present invention, methods and applications have passed through preferred embodiment and have carried out
Description, related personnel can obviously not depart from the content of present invention, carried out in spirit and scope to method described herein and application
Change or appropriate changes and combinations, carry out implementation and application the technology of the present invention.
Just a kind of Chinese medical extract provided by the present invention and its preparation method and application is described further below.
Embodiment 1
Hu Ci Aralia wood 500g is weighed, the ethyl alcohol heating and refluxing extraction 40min that 5000mL volume fraction is 75%, alcohol extracting is added
After filter, filtrate is concentrated into the 1/5 of original volume to obtain the final product.
Embodiment 2
Hu Ci Aralia wood 500g is weighed, ethyl alcohol heating and refluxing extraction 2 times that 3000mL volume fraction is 60% are added, every time
60min is filtered after alcohol extracting, and filtrate is concentrated into the 1/3 of original volume to obtain the final product.
Embodiment 3
Hu Ci Aralia wood 500g is weighed, ethyl alcohol heating and refluxing extraction 3 times that 1500mL volume fraction is 95% are added, every time
30min is filtered after alcohol extracting, and filtrate is concentrated into the 2/5 of original volume to obtain the final product.
Embodiment 4
Hu Ci Aralia wood 500g, Common Leafflower Herb 500g, Canton love-pea vine 500g are weighed, the ethyl alcohol that 6000mL volume fraction is 80% is added
Heating and refluxing extraction 2 times, each 30min is filtered after alcohol extracting, filtrate be concentrated into the 2/5 of original volume up to Hu Ci Aralia wood extract,
Hu Ci Aralia wood extract, Cacumen Securinegae Suffruticosae extract and Herba Abri extract are pressed 10:1 by Cacumen Securinegae Suffruticosae extract and Herba Abri extract:
1 proportion by weight mixes to get the Chinese medicine composition containing Hu Ci Aralia wood extract.
Embodiment 5
Hu Ci Aralia wood 500g, Common Leafflower Herb 500g, Canton love-pea vine 500g are weighed, the second that 15000mL volume fraction is 75% is added
Alcohol heating and refluxing extraction 2 times, each 45min is filtered after alcohol extracting, and filtrate is concentrated into the 1/3 of original volume and extracts up to Hu Ci Aralia wood
Object, Cacumen Securinegae Suffruticosae extract and Herba Abri extract press Hu Ci Aralia wood extract, Cacumen Securinegae Suffruticosae extract and Herba Abri extract
The proportion by weight of 20:3:3 mixes to get the Chinese medicine composition containing Hu Ci Aralia wood extract.
Embodiment 6
Hu Ci Aralia wood 500g, Common Leafflower Herb 500g, Canton love-pea vine 500g are weighed, the ethyl alcohol that 9000mL volume fraction is 60% is added
Heating and refluxing extraction 3 times, each 40min is filtered after alcohol extracting, filtrate be concentrated into the 1/3 of original volume up to Hu Ci Aralia wood extract,
Hu Ci Aralia wood extract, Cacumen Securinegae Suffruticosae extract and Herba Abri extract are pressed 15:2 by Cacumen Securinegae Suffruticosae extract and Herba Abri extract:
2 proportion by weight mixes to get the Chinese medicine composition containing Hu Ci Aralia wood extract.
Embodiment 7
This research purpose be using Hu Ci Aralia wood extract be raw material, verify its have Porcine epidemic diarrhea virus is resisted
Virus function.Cause the main marcy agent (TGEV) that is infectious of the virus of pig virus diarrhoea, rotavirus (RV) and stream
Three kinds of row diarrhea virus (EDV).Although others are also possible to cause pig virus diarrhoea, these three viruses are main
The cause of disease.Test result will provide reference in selection of clinical prevention and control pig virus drug for Hu Ci Aralia wood, more develop peace
Complete effective anti-swine disease cytotoxic drug based theoretical.
The screening test of porcine epidemic diarrhea resisting virus drugs is divided into two big steps, and the first step is that drug tries the toxicity of cell
It tests, to determine that drug limits the quantity to the toxic effect and maximal non-toxic of cell；Second step is the Antiviral screening test of drug.Wherein
The first step is to do the premise of second step test, and it is right to exclude drug itself using the drug progress antivirus test of safe concentration
The toxic effect of cell keeps result more accurate and reliable.Screening test can be divided into following components again: (one) is with 50% Damnacamthus indicus
Preliminary screening as antivirus action of the Aralia wood extracting solution (being prepared in embodiment 2, similarly hereinafter) as maximum safe concentration
Test.(2) its mechanism of action is explored, is administered in four manners respectively: be administered after first virus infection, be first administered and feel afterwards
Catch an illness poison, virus and drug and meanwhile give act on a period of time after cell, virus and drug mixing after give cell again, it is preliminary true
Determine drug effect link.This test result provides the application for treating swine influenza virus infection for Chinese medicine to scientific basis, for safety
The exploitation based theoretical of effective anti-swine flu virus drugs, also the treatment for clinical medicine influenza provides important evidence.
1, porcine epidemic diarrhea resisting Viral safety is tested
Cell is given with 50% Hu Ci Aralia wood extracting solution, test different pharmaceutical is thin to MDCK to cytotoxic effect and drug
The maximum safe concentration of born of the same parents.The specific method is as follows: with preparing in 96 orifice plates well-grown first cell monolayer.Respectively by Hu Ci Aralia
The wooden extracting solution is serially diluted with cell maintenance medium 1:2 multiple proportions.The drug of the various concentration diluted is added to the cell of 96 orifice plates
In single layer, 5 repetitions of each dilution, while normal control hole is set, cell maintenance medium is added in hole in normal control.96 orifice plates
Holes around is filled with sterile PBS.Add and is placed on 37 DEG C, 5%CO2Culture in cell incubator.Day by day result is observed and recorded.
After culture 48 hours, observe and record cytopathy situation under the microscope, and with mtt assay measurement cell viability (MTT colorimetric method,
It is a kind of method for detecting cell survival and growth.Its testing principle is outside the succinate dehydrogenase in living cells mitochondria can make
The bluish violet crystallization that property MTT in source is reduced to water-insoluble and is deposited in cell first a ceremonial jade-ladle, used in libation (Formazan), and dead cell is then not
Can).
Drug maximum safe concentration determines method: universal research microscope observes result production and MTT surveys cell viability knot
Fruit, after Hu Ci Aralia wood extracting solution is added visible cell show as cell it is complete, uniformly, cell monolayer it is complete, will not cell generate
Cytopathy, medicine feeding hole and Normal group difference are small, therefore the dilution of Hu Ci Aralia wood extracting solution 50% is the peace of the drug
Full concentration.
2, porcine epidemic diarrhea resisting virus efficiency assay
Virus lacks complete enzyme and energy system, the metabolism and energy that can only rely on host's living cells synthesize the core of itself
Acid and protein, could complete the duplication of itself.Therefore screening model is established to have to provide the work for supporting viral life first
Animal body or histocyte, the characteristics of comprehensively considering each class model, screening efficiency, cost, operability, this experiment is selected altogether
With two kinds of filtering modes, the internal filtering mode of difference and in-vitro screening mode.
2.1, the outer filtering mode test of porcine epidemic diarrhea resisting virion
Cell selected by in-vitro screening mode is dog renal epithelial cell (MDCK), is continuous cell line, can be in vitro
Continuous passage, and keep biological character to stablize, while being the sensitive cells of pig infection epidemic diarrhea virus.Virus can be
It is proliferated in cell, and generates cytopathy (CPE).The effect of CPE caused by virus can be observed by microscope.This experiment
Using the DMEM culture medium of 5% serum-concentration, condition of culture are as follows: 37 DEG C, 5%CO2, relative humidity 80%.
Experimental method: preparing cell monolayer in 96 orifice plates first, when cell grow to 70% be paved with bottom hole when for testing.
With cell maintenance medium respectively by Hu Ci Aralia wood extracting solution (being prepared in embodiment 2) and Chinese medicine containing Hu Ci Aralia wood extract
Composition (embodiment 6 is made) is diluted to 10% concentration, will be in medical fluid in addition 96 orifice plates, 6 holes of repetition, every 100 μ L of hole, simultaneously
Virus control, normal cell controls and Ribavirin positive control are set.It is observed that lesion in virus inoculation for 24 hours
Occur, is mainly shown as that culture single layer vacuole increases at this time, larger compared with normal cell on cell size, when 48h occurs
Cell rounding, deformation (some is in spindle shape), intercellular gap increases, and phenomena such as cell detachment then occurs within 72 hours.For
The cell that Hu Ci Aralia wood extracting solution is not added is substantially all the cell for the state of lesion occur, and Hu Ci Aralia wood extracting solution is added
Cell be then kept substantially normally, Ribavirin positive controls cell produces deformation, illustrates that Ribavirin has cell
Stronger toxicity, and Hu Ci Aralia wood extracting solution is not only small to cytotoxic but also antiviral effect is good.
The antiviral effect of 1 different role mode drug of table
In order to further explore the Antiviral Mechanism of drug, the cell in every group of 6 holes is got out according to above method, by changing
Become administration time and mode, carry out four groups of processing, while the control group that do not take medicine and the positive for taking Ribavirin are set respectively
Control group is administered after elder generation's virus infection, postoperative infection virus, virus and drug is first administered while giving cell, virus and drug
Cell is given again after acting on a period of time after mixing.The variation for observing cell and the effect after the addition of Hu Ci Aralia wood extracting solution, knot
Fruit is as follows:
(1), drug treatment, then virus infection are first taken for well-grown cell monolayer, after viruses adsorption 1.5h,
Virus liquid is discarded, supplement cell grows maintaining liquid, measurement result, and discovery Hu Ci Aralia wood extracting solution can preferably play antiviral work
With.
(2), for well-grown cell monolayer, first virus infection, viruses adsorption 1.5h is discarded, and gives the maintenance of drug containing
Liquid, final measurement result, discovery Hu Ci Aralia wood extracting solution can preferably play antivirus action.
(3), for well-grown cell monolayer, while drug and virus are fed, final measurement result finds Hu Ci Aralia
The wooden extracting solution can preferably play antivirus action.
(4), effect 2h adds cell after drug is mixed with virus, and final measurement result finds Hu Ci Aralia wood extracting solution
Antivirus action can preferably be played.
By testing above, 10% concentration Hu Ci Aralia wood extracting solution (preparing in embodiment 2) is found and containing tiger
The Chinese medicine composition (embodiment 6 is made) of Ci Aralia wood extract is used respectively at the different time of virus infection, is had preferable
Antivirus action.Control group without medication does not have any improvement with the control group substantially symptom for taking Ribavirin, says
It is bright in this experiment, Hu Ci Aralia wood extracting solution have preferable antiviral effect, and antiviral property be far superior to Western medicine benefit
Ba Weilin.It is shown in Table 2:
The antiviral effect of table 2, different role mode drug
Note: in same row, antivirus action can be played in the processing mode is denoted as "+", cannot play antiviral
Effect is denoted as "-", and comprehensive statistics result is the comprehensive statistics that comprehensive 6 total four kinds of processing modes play antivirus action.
2.2, filtering mode is tested in porcine epidemic diarrhea resisting virion
Sow, boar and piglet each 100 are respectively chosen in the pig farm of infection epidemic diarrhea virus respectively, with Hu Ci Aralia
The wooden extracting solution (preparing in embodiment 2) is fabricated to particle as sole active agent and is respectively added in pannage and contains
There is the Chinese medicine composition (embodiment 6 is made) of Hu Ci Aralia wood extract to be fabricated to particle as only active constituent to add respectively
Into pannage, the Hu Ci Aralia wood extract particles or 10g in 1000 grams of feeds containing 10 grams is made to contain Hu Ci Aralia wood extract
Chinese medicine composition is continuously fed five days to susceptible pig, and daily observation is caught an illness as a result, set up control group and positive controls simultaneously
But sow, boar and the piglet not being administered each 100；Sow is chosen simultaneously, boar and piglet each 100 feed Li Bawei
Woods.It is shown in Table 3:
Table 3, different virus infection pig take the effect after Hu Ci Aralia wood extract
Note: in same row, antivirus action can be played in the processing mode is denoted as "+", cannot play antiviral
Effect is denoted as "-", and comprehensive statistics result is the comprehensive statistics of comprehensive 100 pig antivirus actions.
By upper table 3 it is found that control group 1 is not due to adding medicine, the boar, sow and piglet for infecting epidemic diarrhea virus exist
After being observed continuously 5 days, symptom does not all mitigate；After control group 2 has taken Ribavirin, first three days symptom does not mitigate, and arrives
The symptom of 4th -day-old of pig starts to be mitigated；And Hu Ci Aralia wood extract or the Chinese medicine containing Hu Ci Aralia wood extract are taken
Composition is added in pannage in infection epidemic diarrhea virus boar, sow and piglet substantially just effective from the 2nd day
Fruit, and daily symptom is all mitigated, and shows Hu Ci Aralia wood extract or the Chinese traditional medicine composition containing Hu Ci Aralia wood extract
Object has good anti-epidemic diarrhea virus effect.The above description of test, Hu Ci Aralia wood extract of the present invention or contains
The Chinese medicine composition of Hu Ci Aralia wood extract and the feed being added in pig feed can be reasonably resistant to Porcine Epidemic Diarrhea
Viral disease, and have it is good prevention Porcine epidemic diarrhea virus disease generation, and Hu Ci Aralia wood extract of the present invention or
The antiviral effect of Chinese medicine composition containing Hu Ci Aralia wood extract is better than the effect of existing common Western medicine, therefore, the present invention
The Hu Ci Aralia wood extract or Chinese medicine composition containing Hu Ci Aralia wood extract can avoid pig farm infection popular well
Diarrhea virus.
Under natural conditions, swinery occurrence regularity is often that individual pigs show clinical symptoms first, is not giving treatment
In the case of, then cause a large amount of pig infection in swinery rapidly and fall ill.In the farm of scale, a small number of pig senses are often found
Drug therapy is just given after dye, this is a small number of infection outside toxin expellings of pig, in addition the open country poison invasion in environment, therefore most of
Pig contact virus and the mode of drug are while contacting.Therefore prompt, should practice in answer close observation detect swinery health status,
It is treated in time with active drug as early as possible, is the key measure for controlling swine flu.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (4)
1. Hu Ci Aralia wood extract or the Chinese medicine composition containing Hu Ci Aralia wood extract have anti-pig virus diarrhoea in preparation
Application in the product of effect；
The Chinese medicine composition containing Hu Ci Aralia wood extract is Hu Ci Aralia wood extract, Cacumen Securinegae Suffruticosae extract and Canton love-pea vine
Extract, the proportion by weight of the Hu Ci Aralia wood extract, Cacumen Securinegae Suffruticosae extract and Herba Abri extract be 10~20:1~
3:1~3；
The Hu Ci Aralia wood extract is prepared by the following method acquisition: choosing Hu Ci Aralia wood, ethyl alcohol is added after crushing, heat
It is filtered after refluxing extraction, after filtrate is concentrated to obtain the final product；
The Chinese medicine composition containing Hu Ci Aralia wood extract is prepared by the following method acquisition: choosing Hu Ci Aralia wood, leaf
Lower pearl and Canton love-pea vine are separately added into ethyl alcohol, filter after heating and refluxing extraction after crushing, mention after filtrate is concentrated up to Hu Ci Aralia wood
Object, Cacumen Securinegae Suffruticosae extract and Herba Abri extract are taken, Hu Ci Aralia wood extract, Cacumen Securinegae Suffruticosae extract and Herba Abri extract are pressed
Above-mentioned proportion by weight mixes to get the Chinese medicine composition containing Hu Ci Aralia wood extract.
2. application according to claim 1, it is characterized in that: the proportion by weight of the Hu Ci Aralia wood and ethyl alcohol be 1:3~
10, wherein the volumn concentration of ethyl alcohol is 30~95%, and each extraction time is 30~60min, and extraction time is 1~3 time,
Concentrate the filtrate to the 1/5~2/5 of filtrate original volume.
3. application according to claim 1, it is characterized in that: Hu Ci Aralia wood, Common Leafflower Herb and the Canton love-pea vine and ethyl alcohol
Proportion by weight is 1:3~10, and the volumn concentration of ethyl alcohol is 30~95%, and each extraction time is 30~60min, is mentioned
Taking number is 1~3 time, concentrates the filtrate to the 1/5~2/5 of filtrate original volume to obtain the final product.
4. application according to claim 1, it is characterized in that: the product is additive, drug or feed, the feed
Chinese medicine composition and pig basal feed including Hu Ci Aralia wood extract or containing Hu Ci Aralia wood extract, wherein Hu Ci Aralia wood mentions
Taking the proportion by weight of object or Chinese medicine composition and pig basal feed containing Hu Ci Aralia wood extract is the .2:20 of 0 .8~1.
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Citations (2)
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| CN102512485A (en) * | 2011-12-31 | 2012-06-27 | 陕西中医学院 | Traditional Chinese medicine for treating acute hepatitis, chronic hepatitis and hepatic fibrosis |
| CN104474148A (en) * | 2015-01-16 | 2015-04-01 | 杜卫兵 | Traditional Chinese medicine composition used for treating dermatomycosis and preparation method thereof |
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|---|---|---|---|---|
| CN102512485A (en) * | 2011-12-31 | 2012-06-27 | 陕西中医学院 | Traditional Chinese medicine for treating acute hepatitis, chronic hepatitis and hepatic fibrosis |
| CN104474148A (en) * | 2015-01-16 | 2015-04-01 | 杜卫兵 | Traditional Chinese medicine composition used for treating dermatomycosis and preparation method thereof |
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| Title |
|---|
| "PLANTS USED MEDICINALLY BY FOLK HEALERS OF THE LAHU PEOPLE FROM THE AUTONOMOUS COUNTY OF JINPING MIAO,YAO, AND DAI IN SOUTHWEST CHINA";Hu-YIN HUAI等;《economic botany》;20030317;第58卷;第s265-s273页 * |
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