CN105850721A - Breeding method for heatproof carrot cytoplasmic male sterile line - Google Patents
Breeding method for heatproof carrot cytoplasmic male sterile line Download PDFInfo
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- CN105850721A CN105850721A CN201610276375.XA CN201610276375A CN105850721A CN 105850721 A CN105850721 A CN 105850721A CN 201610276375 A CN201610276375 A CN 201610276375A CN 105850721 A CN105850721 A CN 105850721A
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- radish
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- sterile line
- carrot
- cytoplasmic male
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The invention discloses a breeding method for a heatproof carrot cytoplasmic male sterile line. The method comprises the following steps that a carrot cytoplasmic male sterile line 262A is used as a female parent, and a seven-leaf carrot selfing line is used as a male parent for hybridization; at the flowering phase, several male parent stock trees and female parent stock trees under good growing conditions are selected for tagging and bagging, pairing pollination is conducted, and meanwhile male parent selfing seeds are reserved; in the next year, obtained hybrid seeds and the male parent selfing seeds are planted separately and observed, single plants with the selection traits tending to the male parent and the flowering phase sterility reaching 100% are selected, a corresponding seven-leaf carrot selfing line offspring is used as a recurrent parent for backcross for five generations, and then a sterile line highly similar to seven-leaf carrots and a maintainer line of the sterile line are selected, namely the seven-leaf carrot male sterile line YHR2013A and the maintainer line YHR2013B thereof. The heatproof carrot cytoplasmic male sterile line can serve as carrot heatproof parent seeds, and if the heatproof carrot cytoplasmic male sterile line and an excellent male parent line are used for hybrid seed production, a new radish variety with high heat resistance, a high yield and high quality can be cultivated.
Description
Technical field
The present invention relates to the selection of a kind of heat resistant type red skin radish cytoplasmic male sterility, belong to field of plant breeding.
Background technology
It is bigger that red skin radish saves cultivated area in China Hubei, Jiangsu, Shandong, Sichuan, Guizhou, Yunnan etc., main based on plantation autumn and winter, kind is abundanter, has Hybrid also to have conventional variety (" form of China's main radish local varieties and type " connects brave, Zhang Jizeng " China's Vegetable " the 6th phase in 1996).As the reddest in red rich one, No. two radish in Shenyang, red 3, No. 4 radish in capital, Pekinese, Chengdu and Nanjing clovershrub radish etc., and summer and spring cultivated area less, of less types, the kind of plantation in summer only has the conventional varieties such as seven leaf carrots (" early radish " seven leaves are red " " danger Xinhua's phase " the Changjiang river vegetables " nineteen ninety the 2nd), carrot in the mid-autumn, show as the problems such as heat resistance is strong, yield is relatively low, seed purity is the highest, lack that heat resistance is strong, yield is high, the cenospecies of quality better.
In recent years; along with growing vegetables breed structure readjustment and people's consumption demand change; vegetables in improper season kind radish planting area increases day by day; on the one hand by protection facility or the weather conditions of high mountain height above sea level; popularizing planting anti-season radish, on the other hand passes through radish Germplasm enhancement, utilizes heterosis, hybrid vigor to obtain strong stress resistance kind, comparatively speaking; later approach more can reduce planting cost, can avoid because high mountain plantation causes the destruction to ecological environment simultaneously.Therefore, in order to cultivate the heat-resisting radish varieties of resistance, it is necessary to obtain heat-resisting sterile line, by formulated in combination, combining ability test, heat-resisting radish new varieties are selected, the hot radish varieties of routine promoted in the market with replacement.
Carrot kind is caused to be difficult to the main cause planted under the conditions of summer high temperature: one is summer temperature height, high humidity, and pest and disease damage occurrence degree is high, and general radish varieties is difficult in adapt to harsh climate condition；Two is that conventional radish varieties gene is single, amixia advantage, it is impossible to be polymerized multiple proterties, form the advantage that heat resistance is good, yield is high.
It is therefore desirable to work out the selection of a kind of heat resistant type red skin radish cytoplasmic male sterility, to overcome the problems referred to above.
Summary of the invention
It is an object of the invention to overcome the defect of prior art, it is provided that the selection of a kind of heat resistant type red skin radish cytoplasmic male sterility, its sterile line cultivated has the beneficial effects such as heat resistance is strong, coordinate force is high, the production of hybrid seeds is easy.
The present invention is achieved in that
The present invention provides the selection of a kind of heat resistant type red skin radish cytoplasmic male sterility, comprises the following steps: step one: with radish cytoplasmic male sterility 262A as female parent, with seven leaf carrot self-mating systems for male parent seeding and seedling raising respectively；Step 2: seedling age is after 40-50 days, and field planting to separation net is indoor, flower bud phase bagging, and marks；Step 3: when the florescence, carries out artificial hybridization, takes paternal plant pollen and pollinates to maternal plant, and male parent carries out selfing and reserves seed for planting simultaneously；Step 4: hybrid seed and the corresponding male parent selfed seed of acquisition are sowed by Second Year respectively, nursery field planting, eliminate characters of progenies and fertility has separation combination, therefrom selection traits deflection male parent, florescence sterile rate reach the individual plant of 100%, with seven leaf carrot self-mating systems as recurrent parent, backcross after 5 generations, i.e. obtain sterile line YHR2013A and corresponding holding is YHR2013B；Step 5: be YHR2013B original seed seeding and seedling raising by sterile line YHR2013A and corresponding holding thereof, seedling age 40-50 days, and select, the radish field planting chosen to separation net is indoor, it is that YHR2013B radish field planting is on the middle furrow in solarium by holding, sterile line YHR2013A radish field planting is on the furrow of both sides, radish seed after results is new sterile line YHR2013A, can be as red skin radish heat resistanceheat resistant parent material, carry out hybrid seeding with excellent male parent system, the red skin radish new varieties that heat resistance is strong, yield is high, quality is excellent can be cultivated.
Further, in step 5, holding is YHR2013B radish and the field planting ratio of sterile line YHR2013A radish is 1:2.
Further, in step 5, radish kind strain combines to loosen the soil after surviving and carries out weeding, ridging, carried out pouring water and the prevention and control of plant diseases, pest control, after the beginning of spring in next year, carry out weeding and the prevention and control of plant diseases, pest control before the winter.
Further, 1000 times of liquid of 10% imidacloprid were used to carry out the prevention and control of plant diseases, pest control before winter, after the beginning of spring in next year, 58% 500-600 times of liquid of metalaxyl wettable powder, 1500 times of liquid of 50% Aphox wettable powder and 1500 times of liquid of 1.8% abamectin emulsifiable concentrate are used to carry out the prevention and control of plant diseases, pest control.
Further, before the strain of radish kind is bloomed, it is ensured that solarium, without leak, uses apis mellifera Linnaeus to pollinate, apis mellifera Linnaeus, before putting into solarium, needs first to place 3-5 days in rape, Chinese cabbage or wild cabbage solarium.
Further, within before putting honeybee 3-5 days, carrying out the prevention and control of plant diseases, pest control the most thoroughly, until end of blooming no longer carries out spraying medicine after putting honeybee, in finding solarium such as midway, pest and disease damage occurs serious, can remove honeybee, place into honeybee after laxative.
The method have the advantages that
The heat resistant type red skin radish cytoplasmic male sterility selection provided by the present invention, the sterile line cultivated has the advantages that
1, having heat resistance strong, during the production of hybrid seeds, kind strain growing way is prosperous；
2, coordinate force is high, can make backbone parent, make the combination meeting heat resistanceheat resistant radish breeding objective；
3, overcoming former sterile line bud aetiolation, hybrid seed yield substantially increases.
Detailed description of the invention
Technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described embodiment is only a part of embodiment of the present invention rather than whole embodiments.Based on the embodiment in the present invention, all other embodiments that those of ordinary skill in the art are obtained on the premise of not making creative work, broadly fall into the scope of protection of the invention.
The embodiment of the present invention provides the selection of a kind of heat resistant type red skin radish cytoplasmic male sterility, utilize " China's Vegetable " the 6th phases in 2006 such as existing radish cytoplasmic male sterility 262A(" radish male sterile cytoplasm is identified and classification " Zhang Li), red for transformation parent material with existing hot radish varieties seven leaf without recovering male sterility ability, after hybridizing with 262A, after backcrossing for 5 generations with seven leaf carrot kinds for recurrent parent again, form a neoplasm male sterile line, code name is YHR2013A, this sterile line is in addition to florescence male flower is sterile without powder, all other features are similar to the former transformation parent seven red height of leaf, variable rate technology is precocious, heat resistance is strong, florescence, sterile rate reached 100%, coordinate force is high, it it is a more satisfactory heat-resisting red skin radish male sterile.
Sterile line 262A is the cytoplasmic male sterile line of a stable fertility, it is mainly characterized by: in ripe, plant type half is upright, floral leaf, vein redness, the short circle of radish, skin light red, meat white, when blooming, staminody, gynoecium are normally, sterile rate 100%, after excellent male parent hybridization between selfed lines, its first-filial generation advantage is strong.
The seven red self-mating systems of leaf are red as material with Hubei Province's local varieties seven leaf, isolate, through strict selfing, selection of reserving seed for planting and form, and its feature is: plant type half is upright, leaf is plate leaf, and the number of sheets is few, and fleshy root is circular, red skin plain boiled pork, root length 6-7cm, the thick 5-6cm of root, single heavy 150-200g, precocious, heat-resisting, 40-50 days breeding time, variable rate technology is disease-resistant, growing way is prosperous.
Radish self-mating system is utilized to backcross for recurrent parent, continuous backcross 4-5 generation, obtain new round carrot cytoplasmic male sterile line YHR2013A and corresponding holding is YHR2013B, this sterile line feature that not only heredity seven leaves are red, but also have that sterile rate reaches 100%, florescence bud xanthate ratio is low, plant the features such as strain growth potential is strong.
Sterile line YHR2013A blade profile is plate leaf, and there is shallow incising at edge, and fleshy root is circular, and epidermis is red, meat white, and strong stress resistance plants strain growth vigorous.
The detailed technology measure of the selection of described heat resistant type red skin radish cytoplasmic male sterility is as follows:
Step one: with radish cytoplasmic male sterility 262A as female parent, with seven leaf carrot self-mating systems for male parent seeding and seedling raising respectively.The selection loam that irrigation and drainage are convenient to both, physical features is higher or sandy loam make seedbed, it is desirable to soil layer is deep, loose fertile, and front stubble is avoided and crop in cruciferae continuous cropping, is advisable with legume.Harrowing broken leveling before broadcasting, every 667m2 executes NPK about 40kg, opens railway carriage or compartment bedding by 1.5m, it is desirable to furrow face is smooth.Ditch by 30cm, ditch depth 3-5cm, waters drilling after the water of vola, covers with fine earth after broadcasting in ditch.
Step 2: seedling age is after 40-50 days, and field planting to separation net is indoor, first by solarium's cleaning sterilization, opens railway carriage or compartment bedding by 1-1.2m, executes NPK 40kg, boron fertilizer 1-2kg by every 667 m2 during whole ground, flatten after mixing.Flower bud phase bagging, and mark.
Step 3: when the florescence, carries out artificial hybridization, takes paternal plant pollen and pollinates in pairs to maternal plant, and male parent carries out selfing and reserves seed for planting simultaneously, independent threshing, pack after seed maturity.
Step 4: hybrid seed and the corresponding male parent selfed seed of acquisition are sowed by Second Year respectively, nursery field planting, eliminate characters of progenies and fertility has separation combination, therefrom selection traits is partial to male parent, florescence sterile rate reaches 100%, xanthate ratio is low, plant the individual plant that strain growing way is strong, with corresponding seven leaf carrot self-mating systems as recurrent parent, backcross after 5 generations, i.e. obtain sterile line YHR2013A and corresponding holding is YHR2013B.
Step 5: be YHR2013B original seed seeding and seedling raising by sterile line YHR2013A and corresponding holding thereof, seedling age 40-50 days, and select, the radish field planting chosen to separation net is indoor, it being that YHR2013B(makees male parent by holding) radish field planting is in the middle of the solarium on furrow, sterile line YHR2013A(makees female parent) radish field planting is on the furrow of both sides, plantation density is 3000 strains/667m2, wherein, holding is YHR2013B radish and the field planting ratio of sterile line YHR2013A radish is 1:2, i.e. field planting 1 furrow keep is YHR2013B radish, 2 furrow sterile line YHR2013A radish.After radish field planting, radish kind strain combines to loosen the soil after surviving and carries out weeding, ridging, carried out pouring water and the prevention and control of plant diseases, pest control, after the beginning of spring in next year, carry out weeding and the prevention and control of plant diseases, pest control before the winter.Use 1000 times of liquid of 10% imidacloprid to carry out the prevention and control of plant diseases, pest control before winter, after the beginning of spring in next year, use 58% 500-600 times of liquid of metalaxyl wettable powder, 1500 times of liquid of 50% Aphox wettable powder and 1500 times of liquid of 1.8% abamectin emulsifiable concentrate to carry out the prevention and control of plant diseases, pest control.Before radish kind strain is bloomed, it is ensured that solarium, without leak, uses apis mellifera Linnaeus to pollinate, apis mellifera Linnaeus, before putting into solarium, needs first to place 3-5 days in rape, Chinese cabbage or wild cabbage solarium.Within before honeybee 3-5 days, carrying out the prevention and control of plant diseases, pest control the most thoroughly, until end of blooming no longer carries out spraying medicine after putting honeybee, in finding solarium such as midway, pest and disease damage occurs serious, can remove honeybee, place into honeybee after laxative.
After radish kind pod maturation, harvesting in time, it is bundled in rake ties up to solarium and carries out after-ripening, threshing, radish seed after results is new sterile line YHR2013A, can be as red skin radish heat resistanceheat resistant parent material, carry out hybrid seeding with excellent male parent system, the red skin radish new varieties that heat resistance is strong, yield is high, quality is excellent can be cultivated and plant popularization in summer.
By the selection of the heat resistant type red skin radish cytoplasmic male sterility that the present invention provides, the new sterile line YHR2013A cultivated, except maintaining that former sterile line male sterility is stable, in addition to the feature of sterile rate 100%, also has the advantage that
1, having heat resistance strong, during the production of hybrid seeds, kind strain growing way is prosperous；
2, coordinate force is high, can make backbone parent, make the combination meeting heat resistanceheat resistant radish breeding objective；
3, overcoming former sterile line bud aetiolation, hybrid seed yield substantially increases.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.
Claims (6)
1. the selection of a heat resistant type red skin radish cytoplasmic male sterility, it is characterised in that comprise the following steps:
Step one: with radish cytoplasmic male sterility 262A as female parent, with seven leaf carrot self-mating systems for male parent seeding and seedling raising respectively；
Step 2: seedling age is after 40-50 days, and field planting to separation net is indoor, flower bud phase bagging, and marks；
Step 3: when the florescence, carries out artificial hybridization, takes paternal plant pollen and pollinates to maternal plant, and male parent carries out selfing and reserves seed for planting simultaneously；
Step 4: hybrid seed and the corresponding male parent selfed seed of acquisition are sowed by Second Year respectively, nursery field planting, eliminate characters of progenies and fertility has separation combination, therefrom selection traits deflection male parent, florescence sterile rate reach the individual plant of 100%, with seven leaf carrot self-mating systems as recurrent parent, backcross after 5 generations, i.e. obtain sterile line YHR2013A and corresponding holding is YHR2013B；
Step 5: be YHR2013B original seed seeding and seedling raising by sterile line YHR2013A and corresponding holding thereof, seedling age 40-50 days, and select, the radish field planting chosen to separation net is indoor, it is that YHR2013B radish field planting is on the middle furrow in solarium by holding, sterile line YHR2013A radish field planting is on the furrow of both sides, radish seed after results is new sterile line YHR2013A, can be as red skin radish heat resistanceheat resistant parent material, carry out hybrid seeding with excellent male parent system, the red skin radish new varieties that heat resistance is strong, yield is high, quality is excellent can be cultivated.
The selection of a kind of heat resistant type the most as claimed in claim 1 red skin radish cytoplasmic male sterility, it is characterised in that: in step 5, holding is YHR2013B radish and the field planting ratio of sterile line YHR2013A radish is 1:2.
The selection of a kind of heat resistant type the most as claimed in claim 1 or 2 red skin radish cytoplasmic male sterility, it is characterized in that: in step 5, radish kind strain combines to loosen the soil after surviving and carries out weeding, ridging, carried out before winter pouring water and the prevention and control of plant diseases, pest control, after the beginning of spring in next year, carry out weeding and the prevention and control of plant diseases, pest control.
The selection of a kind of heat resistant type the most as claimed in claim 3 red skin radish cytoplasmic male sterility, it is characterized in that: before the winter, used 1000 times of liquid of 10% imidacloprid to carry out the prevention and control of plant diseases, pest control, after the beginning of spring in next year, 58% 500-600 times of liquid of metalaxyl wettable powder, 1500 times of liquid of 50% Aphox wettable powder and 1500 times of liquid of 1.8% abamectin emulsifiable concentrate are used to carry out the prevention and control of plant diseases, pest control.
5. the selection of a kind of heat resistant type red skin radish cytoplasmic male sterility as described in claim 1 or 3, it is characterized in that: before radish kind strain is bloomed, guarantee that solarium is without leak, apis mellifera Linnaeus is used to pollinate, apis mellifera Linnaeus, before putting into solarium, needs first to place 3-5 days in rape, Chinese cabbage or wild cabbage solarium.
The selection of a kind of heat resistant type the most as claimed in claim 5 red skin radish cytoplasmic male sterility, it is characterized in that: within before putting honeybee 3-5 days, carry out the prevention and control of plant diseases, pest control the most thoroughly, until end of blooming no longer carries out spraying medicine after putting honeybee, in finding solarium such as midway, pest and disease damage occurs serious, honeybee can be removed, after laxative, place into honeybee.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108887176A (en) * | 2018-09-20 | 2018-11-27 | 中国农业科学院蔬菜花卉研究所 | The OguCMS system of high hybrid seed yield and its holding system selective breeding method |
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| CN104170723A (en) * | 2014-08-12 | 2014-12-03 | 江苏沿海地区农业科学研究所 | Breeding method of heat-resistant red-skin radish |
| CN104719125A (en) * | 2015-03-02 | 2015-06-24 | 江苏沿海地区农业科学研究所 | Heat-resistant circular spherical white radish breeding selection method |
| CN105052728A (en) * | 2015-08-28 | 2015-11-18 | 武汉蔬博农业科技有限公司 | Method for breeding recessive cell nucleus male sterile line of radish and method for breeding heat-resistant radish variety |
| CN105165600A (en) * | 2015-09-23 | 2015-12-23 | 武汉市蔬菜科学研究所 | Cultivation method of round radish cytoplasmic male novel sterile line |
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2016
- 2016-04-29 CN CN201610276375.XA patent/CN105850721A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104170723A (en) * | 2014-08-12 | 2014-12-03 | 江苏沿海地区农业科学研究所 | Breeding method of heat-resistant red-skin radish |
| CN104719125A (en) * | 2015-03-02 | 2015-06-24 | 江苏沿海地区农业科学研究所 | Heat-resistant circular spherical white radish breeding selection method |
| CN105052728A (en) * | 2015-08-28 | 2015-11-18 | 武汉蔬博农业科技有限公司 | Method for breeding recessive cell nucleus male sterile line of radish and method for breeding heat-resistant radish variety |
| CN105165600A (en) * | 2015-09-23 | 2015-12-23 | 武汉市蔬菜科学研究所 | Cultivation method of round radish cytoplasmic male novel sterile line |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108887176A (en) * | 2018-09-20 | 2018-11-27 | 中国农业科学院蔬菜花卉研究所 | The OguCMS system of high hybrid seed yield and its holding system selective breeding method |
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Application publication date: 20160817 |