CN105848477A - Method for producing egg including gold nanoparticles - Google Patents
Method for producing egg including gold nanoparticles Download PDFInfo
- Publication number
- CN105848477A CN105848477A CN201480070849.4A CN201480070849A CN105848477A CN 105848477 A CN105848477 A CN 105848477A CN 201480070849 A CN201480070849 A CN 201480070849A CN 105848477 A CN105848477 A CN 105848477A
- Authority
- CN
- China
- Prior art keywords
- nanometer particle
- golden nanometer
- egg
- water
- gold
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K31/00—Housing birds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K45/00—Other aviculture appliances, e.g. devices for determining whether a bird is about to lay
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K29/00—Other apparatus for animal husbandry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L15/00—Egg products; Preparation or treatment thereof
- A23L15/30—Addition of substances other than those covered by A23L15/20 – A23L15/25
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Environmental Sciences (AREA)
- Birds (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Inorganic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
The present invention relates to a method for producing an egg including gold nanoparticles using water containing gold nanoparticles, and more specifically, to a method for producing an egg including gold nanoparticles using water containing gold nanoparticles, wherein gold particles, which are abraded by a strong water pressure, and sprayed water are stored together, and the stored water is supplied as drinking water for a hen bred in a henhouse, thereby allowing an egg ovulated in the bred hen to include gold nanoparticles. The method for producing an egg including gold nanoparticles using water containing gold nanoparticles of the present invention comprises: a first step of feeding a hen with water containing gold nanoparticles; and a second step of producing an egg by the hen, wherein the produced egg contains 0.50 [mu]g /kg of gold.
Description
Technical field
The present invention relates to the manufacture method of a kind of egg containing golden nanometer particle, more specifically, relate to storage by strong hydraulic pressure
After the gold particle of abrasion and the water of injection, the water of storage is given as drinking water supply and raises the chicken at hen house, thus, in ovulation
Egg in containing the egg manufacture method of golden nanometer particle.
Background technology
During absorption of human body gold, play a part Detoxication and stablize body and mind.But, it is not easy to directly absorb gold, therefore, indirectly
The gold that ground picked-up is contained in food, thus, simply and readily absorb gold.
Korean granted patent the 10-897582nd (2009.05.07) discloses " cultural method of the Fructus Vitis viniferae containing gold nano and gold
Nanometer Fructus Vitis viniferae ", mix gold nano aqueous solution in the Traditional Chinese medicine fertilizer containing Chinese crude drug and sulfur aqueous solution manufactures containing gold nano
Chinese medicine liquid fertilizer, pours water the Chinese medicine liquid fertilizer of manufacture on vine, so that grape fruit contains gold composition.
But existing technology is owing to pouring water on vine containing the Chinese medicine liquid fertilizer of gold, therefore, the amount of soil is remained in more than Portugal
The golden composition that grape tree absorbs.
Further, owing to grape fruit is according to limiting delivery period season, therefore, user is difficult to continuous ingestion, and is difficult to protect
Pipe and mobile grape fruit.
Further, Korean granted patent the 10-0565013rd (2006.03.22)) disclose " obtain nutritional egg chicken feed and
Manufacture method " in order to coordinate, to chicken picked-up gold, the several additives comprising gold in the engineering manufacturing chicken feed.
But, prior art is, according to initial stage chicken, chicken in mid-term, later stage chicken, the initial stage chicken that lays eggs, post-egg stage chicken, in
The classification such as phase chicken, later stage chicken manufactures chicken feed so that replace chicken feed according to raising.Further, join due to multiple compounding ratio
Conjunction several additives is to chicken feed, and therefore, manufacturing engineering is complicated and production unit price also increases.
Summary of the invention
(1) to solve the technical problem that
The present invention proposes in view of described problems, its object is to, it is provided that one completely dispenses with use synthetics
Or the method such as electrolysis, utilize the strong hydraulic pressure sprayed from the nozzle of injection unit to corrode gold so that by the nanoparticle of generation be used for
Corrode the drinking water as chicken after golden water is stored together.
Further, other objects of the present invention are, it is provided that a kind of using the water containing golden nanometer particle as the drinking water of chicken so that
The method for breeding of the chicken of the egg laid eggs containing golden nanometer particle.
Further, other another object of the present invention are, it is provided that a kind of chicken drinking the water containing golden nanometer particle produces egg, from
And containing golden nanometer particle in egg.
Further, other another object of the present invention are, it is provided that a kind of spray strong pure water on proof gold so that can generate nanometer
The gold particle of size, thus, can solve to use other chemical methodes and the side effect that occurs.
(2) technical scheme
It is an object of the invention to, gold sprays pure water so that generate golden nanometer particle according to the corrosion function of gold, so that
The manufacture method of the egg containing golden nanometer particle is manufactured with the pure water containing golden nanometer particle, including: contain to hen picked-up
The first step of the water of described golden nanometer particle and described hen lay eggs the second step of egg, contain in the described egg laid eggs
The gold of 0.50 μ g/.
(3) beneficial effect
The manufacture method of the egg containing golden nanometer particle, when manufacturing golden nanometer particle, does not the most use synthetics or electrolysis
Etc. method so that not environmental pollution.
Further, present invention storage, after the water generating golden nanometer particle and golden nanometer particle, as the drinking water of raising chicken, makes
Obtain and do not comprise other additives or synthetic, thus, the producing cost of the egg containing golden nanometer particle can be reduced.
Further, the present invention keeps the indispensable element of life as the water that chicken picked-up contains golden nanometer particle, needs constantly every day
More than picked-up ormal weight could reduce the content of the golden nanometer particle being contained in water, make chicken absorb golden nanometer particle constantly, thus,
Containing the golden nanometer particle specified in the egg that every day produces.
Further, the present invention sprays strong pure water to generate the gold particle of nanosized on proof gold, thus, can solve to use other
Chemical method and the side effect that occurs.
Accompanying drawing explanation
Fig. 1 is the precedence diagram of the manufacture method for illustrating the egg according to the present invention.
Fig. 2 is for illustrating the microscopical of size according to the gold particle comprised in the water containing golden nanometer particle of the present invention
Picture 1.
Fig. 3 is for illustrating the microscopical of size according to the gold particle comprised in the water containing golden nanometer particle of the present invention
Picture 2.
Fig. 4 is for illustrating the microscopical of size according to the gold particle comprised in the water containing golden nanometer particle of the present invention
Picture 2.
Fig. 5 is the experiment grade book according to the golden amount comprised in the water containing golden nanometer particle of the present invention.
Fig. 6 is the experiment according to the golden amount comprised in the egg utilizing the water containing golden nanometer particle to manufacture of the present invention
Achievement book.
Detailed description of the invention
The term or the word that use in the description of the present invention and right must not limited interpretation be usual or on dictionary
Implication, inventor, in order to the present invention being described by most preferred method, can the most suitably define the concept of term, and want
Meet meaning and the concept of its technological thought.
Further, the embodiment described in the description of the present invention is not limited in the preferred embodiments of the present invention with the structure of drawing,
And do not represent all technological thoughts of the present invention, can replace that the present invention's is various thus, it will be appreciated that can have at the application time point
Deformation and other embodiments of equalization.
Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.
Fig. 1 is the precedence diagram of the manufacture method for illustrating the egg according to the present invention.As it is shown in figure 1, at edible gold
The pure water of upper injection high pressure, utilizes pure water abrasion gold, thus stores the golden nanometer particle of generation and the pure water (S110) of injection together.
That is, if spraying pure water on gold, then gold surface weares and teares into Nanoparticle Size according to the pure water of high pressure, and, the gold of generation
Nanoparticle is included in the pure water of injection.Therefore, after storage is containing the water of golden nanometer particle, the drinking water supply as chicken is given
Chicken.
As shown in Figures 2 to 5, the size of the golden nanometer particle sprayed according to the pure water of high pressure is, consume into 59.5nm, 63.5nm,
65.5nm ... the size of 119nm etc., and it is included in pure water
In, it is preferable that comprise the golden nanometer particle of 40nm to 150nm size.On the other hand, as it is shown in figure 5, utilizing
The gold of the nanosized of about 0.45mg is comprised in the pure water 1kg of the abrasion gold of high-pressure injection.
Therefore, utilize injection high pressure wear and tear gold pure water in comprise 0.25/above golden nanometer particle.
Then, in the water containing golden nanometer particle of storage, sulfur (S120) is added.Sulfur can suppress cholesterol, contributes to
Diabetes and blood pressure, the active oxygen of harmful substance can be reduced, diminish inflammation and sterilize.Therefore, if the water containing sulfur is fed for
The hen laid eggs, then be contained within sulfur component at egg so that provides the effect of multiple beneficial health to the human body of picked-up egg.
The sulfur added in water containing golden nanometer particle is, it is preferable that comprise 1g in the water 1kg containing golden nanometer particle
To 25g, as shown in Figure 6, the sulfur amount of the egg that the hen of the water absorbing the golden nanometer particle being added with sulfur lays eggs is
0.188 weight %.
On the other hand, in the golden nanometer particle water be added with sulfur, add pure water, thus, the dilution water containing golden nanometer particle
(S130).Although the water of the golden nanometer particle being added with sulfur of S120 step can also be fed for chicken, even if but absorbing to chicken
The golden nanometer particle of high-load and sulfur, also can discharge from Excreta, and therefore, pure water more than dilution ormal weight reduces concentration.
Therefore, in the water containing golden nanometer particle, add the pure water of 0.5 times more than to dilute.The amount of the pure water according to dilution can
Change is fed with the period of the water of golden nanometer particle to chicken.
Then, add sulfur, feed the water (S140) containing golden nanometer particle of the dilution pure water of more than 4 weeks to the hen in egg-laying season.
Now, the hen in egg-laying season can drink the water containing golden nanometer particle for 24 hours, thus, no matter when chicken can absorb contains
Having the water of golden nanometer particle, the period of picked-up water is during more than 4 weeks or picked-up to be according to the pure water diluted in S130 step
Amount and different.
Secondly, the hen of the water containing golden nanometer particle absorbing more than 4 weeks lays eggs egg (S150)
As shown in Figure 6, containing 4.35 μ g/ in the egg that the hen of the picked-up water containing golden nanometer particle of more than 4 weeks produces
Gold, containing the sulfur of 0.188 weight % in weight of egg.
Therefore, the gold that the egg that the hen of the picked-up water containing golden nanometer particle of more than 4 weeks produces contains 0.5 more than μ g/
Nanoparticle, and containing sulfur more than 0.01 weight %.
Fig. 2 is for illustrating that first of size according to the gold particle comprised in the water containing golden nanometer particle of the present invention is micro-
The mensuration picture of mirror, Fig. 3 is for illustrating the size according to the gold particle comprised in the water containing golden nanometer particle of the present invention
Second measurement microscope picture and Fig. 4 are for illustrating according to the gold particle comprised in the water comprising golden nanometer particle of the present invention
3rd measurement microscope picture of size.As shown in Figures 2 to 4, after evaporation is containing the water of gold particle, remaining goldc grains is used
Son measures, and in measuring picture, the size of gold particle is 59.5nm to 119nm.
Therefore, in the water containing golden nanometer particle, the golden nanometer particle of detection is 40nm to 150nm.
Fig. 5 is the experiment grade book according to the golden amount comprised in the water containing golden nanometer particle of the present invention.As it is shown in figure 5,
The pure water spraying high pressure on gold erodes so that golden nanometer particle and the pure water of injection that storage generates are stored, and are containing
Have in the water of nanoparticle to analyze and substantially contain how many gold composition.Experiment grade book result, in the water containing golden nanometer particle
The golden composition comprised is 0.45/.
Therefore, the golden nanometer particle detected in the water containing golden nanometer particle is 0.25/.
Fig. 6 is the experiment according to the golden amount comprised in the egg utilizing the water containing golden nanometer particle to manufacture of the present invention
Achievement book.As shown in Figure 6, after adding sulfur, the hen utilizing the water containing golden nanometer particle of dilution pure water to raise lays eggs
Egg in substantially comprise how many gold composition.Experiment grade book result, the water containing golden nanometer particle of sulfur is added in picked-up
The egg laid eggs of hen in the golden composition that comprises be 4.35 μ g/, the sulfur component in egg is 0.188 weight %.
Therefore, the golden composition comprised in the egg that the hen of the water containing golden nanometer particle that sulfur is added in picked-up lays eggs is 0.50
More than μ g/, the sulfur component in egg is more than 0.01 weight %.
As it has been described above, the right of the present invention is not intended to be limited to embodiment described above, should be determined by right,
Carry out various deformation the most within the scope of the claims and amendment is clear and definite.
Claims (8)
1. the manufacture method of the egg containing golden nanometer particle, it is characterised in that spray pure water to gold, according to invading of gold
Erosion effect generates golden nanometer particle, utilizes the manufacture method of the egg of the water containing golden nanometer particle, including: contain to chicken picked-up
The first step of the water of described golden nanometer particle;Described hen lays eggs the second step of egg, contains in the described egg laid eggs
The gold of 0.50 μ g/.
The manufacture method of the egg containing golden nanometer particle the most according to claim 1, it is characterised in that one day 24 little
Time be fed with the water of described golden nanometer particle to described hen.
The manufacture method of the egg containing golden nanometer particle the most according to claim 1, it is characterised in that to the egg-laying season
The water containing described golden nanometer particle fed more than surrounding of described hen manufacture described egg.
The manufacture method of the egg containing golden nanometer particle the most according to claim 1, it is characterised in that containing described
In the water of golden nanometer particle containing 0.25/above golden nanometer particle.
The manufacture method of the egg containing golden nanometer particle the most according to claim 4, it is characterised in that described Jenner
The size of rice corpuscles is 40nm to 150nm.
The manufacture method of the egg containing golden nanometer particle the most according to claim 1, it is characterised in that carry out described
Before first step, in the water containing described golden nanometer particle, dilute the pure water of more than 0.5 times.
The manufacture method of the egg containing golden nanometer particle the most according to claim 6, it is characterised in that dilution is described
Before pure water, the sulfur of 1 to 25g/ is added in the water containing described golden nanometer particle.
The manufacture method of the egg containing golden nanometer particle the most according to claim 7, it is characterised in that described hen
After the water containing described golden nanometer particle of described sulfur is added in picked-up, the described sulfur contained in the described egg laid eggs is
More than 0.01 weight %.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR20130162041A KR101483378B1 (en) | 2013-12-24 | 2013-12-24 | A method of producing eggs containing the gold nanoparticles using a water containing gold nanoparticles |
KR10-2013-0162041 | 2013-12-24 | ||
PCT/KR2014/012771 WO2015099434A1 (en) | 2013-12-24 | 2014-12-23 | Method for producing egg including gold nanoparticles using water containing gold nanoparticles |
Publications (1)
Publication Number | Publication Date |
---|---|
CN105848477A true CN105848477A (en) | 2016-08-10 |
Family
ID=52589128
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201480070849.4A Pending CN105848477A (en) | 2013-12-24 | 2014-12-23 | Method for producing egg including gold nanoparticles |
Country Status (3)
Country | Link |
---|---|
KR (1) | KR101483378B1 (en) |
CN (1) | CN105848477A (en) |
WO (1) | WO2015099434A1 (en) |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20060112083A (en) * | 2005-04-26 | 2006-10-31 | 권석은 | Nutrition solution containing methyl sulfonyl methane, the method of manufacturing the same, the method of raising chicken by feeding the same, the chicken by the method, and egg containing methyl sulfonyl methane |
KR100952982B1 (en) * | 2009-10-20 | 2010-04-15 | 엄기만 | An egg comprising gold and a manufacturing mehtods thereof |
CN102283173A (en) * | 2011-09-21 | 2011-12-21 | 福建省新闽科生物科技开发有限公司 | Production method of eggs with high immune globulin content |
CN103357886A (en) * | 2013-06-28 | 2013-10-23 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method for noble metal nanoclusters of fluorescent sensor |
CN103501812A (en) * | 2011-04-29 | 2014-01-08 | 西莱克塔生物科技公司 | Tolerogenic synthetic nanocarriers for allergy therapy |
CN103737017A (en) * | 2014-01-14 | 2014-04-23 | 山西大学 | Fluorescence gold nanoparticle and preparation method thereof |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20060085777A (en) * | 2005-01-25 | 2006-07-28 | 주식회사 이노바이오 | Additives containing nano type silver for feed and process for preparing it |
KR100890245B1 (en) * | 2007-11-23 | 2009-03-24 | (주)하이드로 비티 | Drinking water composition for poultry farming and preparation method thereof |
KR20110043394A (en) * | 2010-02-08 | 2011-04-27 | 엄기만 | An egg comprising gold and a manufacturing mehtods thereof |
-
2013
- 2013-12-24 KR KR20130162041A patent/KR101483378B1/en active IP Right Grant
-
2014
- 2014-12-23 WO PCT/KR2014/012771 patent/WO2015099434A1/en active Application Filing
- 2014-12-23 CN CN201480070849.4A patent/CN105848477A/en active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20060112083A (en) * | 2005-04-26 | 2006-10-31 | 권석은 | Nutrition solution containing methyl sulfonyl methane, the method of manufacturing the same, the method of raising chicken by feeding the same, the chicken by the method, and egg containing methyl sulfonyl methane |
KR100952982B1 (en) * | 2009-10-20 | 2010-04-15 | 엄기만 | An egg comprising gold and a manufacturing mehtods thereof |
CN103501812A (en) * | 2011-04-29 | 2014-01-08 | 西莱克塔生物科技公司 | Tolerogenic synthetic nanocarriers for allergy therapy |
CN102283173A (en) * | 2011-09-21 | 2011-12-21 | 福建省新闽科生物科技开发有限公司 | Production method of eggs with high immune globulin content |
CN103357886A (en) * | 2013-06-28 | 2013-10-23 | 上海纳米技术及应用国家工程研究中心有限公司 | Preparation method for noble metal nanoclusters of fluorescent sensor |
CN103737017A (en) * | 2014-01-14 | 2014-04-23 | 山西大学 | Fluorescence gold nanoparticle and preparation method thereof |
Also Published As
Publication number | Publication date |
---|---|
WO2015099434A1 (en) | 2015-07-02 |
KR101483378B1 (en) | 2015-01-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Adegbeye et al. | Nanoparticles in equine nutrition: Mechanism of action and application as feed additives | |
Auffan et al. | Role of molting on the biodistribution of CeO2 nanoparticles within Daphnia pulex | |
CN104146880B (en) | A kind of solid spray and preparation method thereof | |
Jeevanandam et al. | Opportunities for nano-formulations in type 2 diabetes mellitus treatments | |
WO2009072845A2 (en) | Functional agricultural product and method for cultivating the same | |
CN105848477A (en) | Method for producing egg including gold nanoparticles | |
CN105104576A (en) | Fruity linum usitatissimum seed oil polypeptide instantly-dissolving powder | |
WO2014058329A1 (en) | Compositions based on hypophyseal hormones for adding to infusion fluids | |
CN108157950A (en) | A kind of strong bone compositions of benefit brain and the spraying device containing it | |
CN208742854U (en) | One kind can smear uniform slushing compound injection device | |
CN105325702A (en) | Gentian H-containing natural antibiotic feed additive as well as preparation and application thereof | |
CN1320376A (en) | Agricultural atrazine-nicosulfuron herbicide | |
CN205515675U (en) | Box subassembly of plant liquid deodorizer | |
CN103271340A (en) | Nanoscale health-care food capable of removing heavy metal in human body and preparation method thereof | |
JP2011142855A (en) | Compound feed for raising flounder and method for raising flounder | |
CN106819449A (en) | Banksia rose nonreactive fattening pannage additive | |
Capdevielle et al. | Effect of acid or aluminum on growth and adrenal function in young chickens | |
de Goes et al. | Dry matter intake of steers supplemented with crambe crushed, in total replacement of soybean meal. | |
KR100952982B1 (en) | An egg comprising gold and a manufacturing mehtods thereof | |
CN205378672U (en) | Deer is raised with multi -functional material trough of throwing | |
KR101911465B1 (en) | Nitrate coating composition for reducing the methane gas produced by the rumen of ruminant Animals and method for producing the same | |
Acton | Chlorides—Advances in Research and Application: 2013 Edition | |
Edalati Nacab et al. | Effect of addition barley on the degradability kinetics, net energy, in vitro drymatter digestibility and metabolizable energy of potato plant silage by gas testtechnique | |
KR101162980B1 (en) | Natural detergent | |
KR200432289Y1 (en) | Nano silver of spary |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160810 |
|
WD01 | Invention patent application deemed withdrawn after publication |