CN105832813A - Method for purifying puerarin in Heshui radix pueraria thomsonii - Google Patents
Method for purifying puerarin in Heshui radix pueraria thomsonii Download PDFInfo
- Publication number
- CN105832813A CN105832813A CN201610356902.8A CN201610356902A CN105832813A CN 105832813 A CN105832813 A CN 105832813A CN 201610356902 A CN201610356902 A CN 201610356902A CN 105832813 A CN105832813 A CN 105832813A
- Authority
- CN
- China
- Prior art keywords
- heshui
- puerarin
- radix puerariae
- temperature
- extracting method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/488—Pueraria (kudzu)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a method for purifying puerarin in Heshui radix pueraria thomsonii. The method comprises the following steps: performing forced air drying on Heshui radix pueraria thomsonii which is cut into blocks for 5 to 7 hours under the temperature of 60 to 80 DEG C, respectively moving samples into a stirrer for crushing, placing the samples into a flask, adding an ethanol solution, performing hot dipping in a water bath pot with the temperature of 75 to 85 DEG C for 1 to 3 hours, then filtering the products, collecting filtrate, adding the ethanol solution into filter residues, performing hot dipping under the same temperature for 1 to 3 hours, filtering the products, combining the two filtrates, performing ethyl alcohol evaporation in the water bath pot with the temperature of 75 to 85 DEG C, putting the combined filtrate into an electrothermal dryer for drying until the weight is constant. The extraction method for the puerarin in the Heshui radix pueraria thomsonii is easy to operate, high in puerarin extraction amount, high in puerarin purity, short in extraction period and low in cost; the economic value of the Heshui radix pueraria thomsonii is increased, and an important reference is provided for industrial extraction of the puerarin in the Heshui radix pueraria thomsonii.
Description
Technical field
The present invention relates to extraction process technology field, particularly relate to Puerarin method of purification in Heshui RADIX PUERARIAE.
Background technology
The root of kudzu vine (Radix puerariae) is legume pueraria lobata Pueraria
Lobate (Willd.) Ohwil or sweet kudzu Pueraria thomsonii Benth. practises the dry root claiming " Pachyrhizua angulatus ".And Puerarin is the endemic element of the root of kudzu vine, it also it is principle active component.Pachyrhizua angulatus is one of Gaoming City local and special products, is distributed mainly on the mountain area towns such as Heshui.Heshui Pachyrhizua angulatus (Heshui Pueraria thomsonii), fiber is tiny, content of starch is high, fresh and sweet matter is crisp.Owing to Heshui soil property is based on loose sandy loam, plants Pachyrhizua angulatus out and feel well sweet without slag, starch rate height, there is higher edible, medical value, as agricultural product most characteristic in the traditional crop of Gaoming District, be one of our province conventional outlet vegetables.The Pueraria lobota sheet of Pachyrhizua angulatus and kudzuvine root starch all can be used as medicine, and have a refreshing and detoxicating, hypoglycemic, effect of relieving the effect of alcohol.The climing work of stem is restricted or weaves, it is possible to make green manure.
Modern age, pharmacological research proved, in the root of kudzu vine, contained active ingredient Pueraria Flavonid (with Puerarin as representative) has reduction MCO, increase coronary artery, brain vessel blood amount, substantially allevating angina pectoris, the pharmacological actions such as anti-arrhythmia, therefore can be made into the medicine for the treatment of diseases of cardiovascular and cerebrovascular systems.In addition, owing to kudzuvine root isoflavone class has anti-oxidant, enhancing immunity of organisms and the melanic generation of human skin can be suppressed to act on formation etc., therefore kudzu root extract can be directly added in the anti-aging of various matrix and freckle whitening makeup as functional cosmetics additive agent, has and develops application potential widely.The progress the biggest currently for the extraction purification of Puerarin and effect, but the melanin nature difference different due to biogenetic derivation is the biggest, corresponding extracting method is also not quite similar, and therefore the most conventional purifying technique cannot effectively extract Puerarin from Heshui RADIX PUERARIAE.
Therefore, prior art needs to improve further.
Summary of the invention
In view of the deficiencies in the prior art, the invention reside in Puerarin method of purification in offer Heshui RADIX PUERARIAE, it is intended to the problem solving effectively to extract Puerarin from Heshui RADIX PUERARIAE without relevant art at present.
Technical scheme is as follows:
The extracting method of Puerarin in a kind of Heshui RADIX PUERARIAE, described method is:
The Heshui RADIX PUERARIAE being cut into bulk is carried out forced air drying about 5-7h, and temperature is 60-80 DEG C, puts in flask after sample is moved to pulverize in mixer respectively, add ethanol solution, hot dipping 1-3h in the water-bath of 75-85 DEG C, then filter, collect filtrate, add ethanol solution in filter residue, at the same temperature, hot dipping 1-3h, filter, merge the filtrate of twice collection, the water-bath of 75-85 DEG C is evaporated ethanol, then put and be dried to constant weight to electric air drier.
The extracting method of Puerarin in described Heshui RADIX PUERARIAE, wherein, described Heshui RADIX PUERARIAE takes from root of kudzu vine skin.
The extracting method of Puerarin in described Heshui RADIX PUERARIAE, wherein, it is 6 h that described Heshui Pachyrhizua angulatus carries out the time of forced air drying, and dry temperature is 70 DEG C.
The extracting method of Puerarin in described Heshui RADIX PUERARIAE, wherein, during extraction, the solid-liquid ratio according to mass ratio 1:8 adds ethanol solution.
The extracting method of Puerarin in described Heshui RADIX PUERARIAE, wherein, the volume fraction of ethanol solution used is 60%.
The extracting method of Puerarin in described Heshui RADIX PUERARIAE, wherein, during extraction, the hot dipping time is 2h.
The extracting method of Puerarin in described Heshui RADIX PUERARIAE, wherein, the temperature that described hot dipping processes is all 80 DEG C with the temperature of the volatilization carrying out ethanol.
Beneficial effect: the present invention provides the extracting method of Puerarin in a kind of Heshui RADIX PUERARIAE, described extracting method is simple to operate, the extracted amount of Puerarin is high and purity is high, extracting cycle is short, the most with low cost, improve the economic worth of Heshui RADIX PUERARIAE, important references is provided for the industrialization of Puerarin in Heshui RADIX PUERARIAE.
Detailed description of the invention
The present invention provides the extracting method of Puerarin in a kind of Heshui RADIX PUERARIAE, and for making the purpose of the present invention, technical scheme and effect clearer, clear and definite, the present invention is described in more detail below.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
The present invention provides the extracting method of Puerarin in a kind of Heshui RADIX PUERARIAE, and described extracting method is:
The extracting method of Puerarin in a kind of Heshui RADIX PUERARIAE, described method is:
First, the Heshui RADIX PUERARIAE being cut into bulk being carried out forced air drying about 5-7h, temperature is 60-80 DEG C, puts in flask after Heshui RADIX PUERARIAE is moved to pulverize in mixer.Block Heshui RADIX PUERARIAE is carried out forced air drying process, removes the moisture in Heshui RADIX PUERARIAE, be conducive to next step process, also allow for preserving.The preferably time of forced air drying is 6 h, and when dry temperature is 70 DEG C, rate of drying is fast, effective, the Puerarin in survivable Heshui RADIX PUERARIAE.
Bulk the footpath of Heshui RADIX PUERARIAE is unsuitable excessive, maximum less than 1mm, if conference is crossed in a footpath causes difficulty to the extraction of sample, if but a diameter is too small bring difficulty also can to follow-up lock out operation.
Preferably, described Heshui RADIX PUERARIAE takes from root of kudzu vine skin, and the Puerarin extracted amount extracted in the root of kudzu vine skin of Heshui Pachyrhizua angulatus is the highest.
Secondly, add ethanol solution, hot dipping 1-3h in the water-bath of 75-85 DEG C, then filter, collect filtrate, add ethanol solution in filter residue, at the same temperature, hot dipping 1-3h, filters, merges the filtrate of twice collection.Preferably, the temperature that hot dipping processes is 80 DEG C, and when the time is 2h, the extracting efficiency of Puerarin is the highest, and does not affect the activity of Puerarin.
Within the specific limits, in Heshui RADIX PUERARIAE, the extracted amount of Puerarin increases with the amount of ethanol solution and improves, but when the amount of ethanol solution is too much, the extracted amount of Puerarin the most no longer changes, the most too much ethanol solution can improve the cost of extraction, also improves the difficulty removing ethanol in purifying.Preferably, when the solid-liquid ratio according to mass ratio 1:8 adds the ethanol solution of 60% in solution, in Heshui RADIX PUERARIAE, the extraction efficiency of Puerarin is the highest, and cost is minimum.
Preferably, the temperature of the volatilization carrying out ethanol is all 80 DEG C, and under this conditional, ethanol volatilization efficiency is the highest.
Finally, the filtrate of above-mentioned collection is processed in the water-bath of 75-85 DEG C, stirring, promote the volatilization of ethanol in filtrate, then put and be dried to constant weight to electric air drier.Water bath processing 2h, the ethanol in Ex-all filtrate.
For the preparation method of the more detailed description present invention, it is exemplified below several embodiment further description.
Embodiment
1
In the Heshui RADIX PUERARIAE that the present invention provides, the extracting method of Puerarin is as follows:
The root of kudzu vine skin being cut into the Heshui RADIX PUERARIAE of bulk is carried out forced air drying about 5h, temperature is 80 DEG C, put in flask after sample is moved to pulverize in mixer respectively, add 50% ethanol solution that solid-liquid ratio is 1:7, hot dipping 3h in the water-bath of 75 DEG C, then filter, collect filtrate, add ethanol solution in filter residue, at the same temperature, hot dipping 3h, filter, merge the filtrate of twice collection, the water-bath of 75 DEG C is evaporated ethanol, then put and be dried to constant weight to electric air drier.
Experimental result shows, in Heshui RADIX PUERARIAE, the yield of Puerarin has 0.94%, and it is 5.4% that Puerarin accounts for extract obtained ratio.
Embodiment
2
In the Heshui RADIX PUERARIAE that the present invention provides, the extracting method of Puerarin is as follows:
The root of kudzu vine skin being cut into the Heshui RADIX PUERARIAE of bulk is carried out forced air drying about 6h, and temperature is 70 DEG C, puts in flask after sample is moved to pulverize in mixer respectively, adding 60% ethanol solution that solid-liquid ratio is 1:8, in the water-bath of 80 DEG C, hot dipping 2h, then filters, collect filtrate, add ethanol solution in filter residue, at the same temperature, hot dipping 2h, filter, merge the filtrate of twice collection, the water-bath of 80 DEG C is evaporated ethanol, then put and be dried to constant weight to electric air drier.
Experimental result shows, in Heshui RADIX PUERARIAE, the yield of Puerarin has 1.03%, and it is 5.9% that Puerarin accounts for extract obtained ratio.
Embodiment
3
In the Heshui RADIX PUERARIAE that the present invention provides, the extracting method of Puerarin is as follows:
The root of kudzu vine skin being cut into the Heshui RADIX PUERARIAE of bulk is carried out forced air drying about 7h, and temperature is 60 DEG C, puts in flask after sample is moved to pulverize in mixer respectively, adding 70% ethanol solution that solid-liquid ratio is 1:9, in the water-bath of 85 DEG C, hot dipping 1h, then filters, collect filtrate, add ethanol solution in filter residue, at the same temperature, hot dipping 1h, filter, merge the filtrate of twice collection, the water-bath of 85 DEG C is evaporated ethanol, then put and be dried to constant weight to electric air drier.
Experimental result shows, in Heshui RADIX PUERARIAE, the yield of Puerarin has 0.96%, and Puerarin accounts for extract obtained ratio and is.
The present invention provides the extracting method of Puerarin in a kind of Heshui RADIX PUERARIAE, described extracting method is simple to operate, the extracted amount of Puerarin is high and purity is high, extracting cycle is short, the most with low cost, improve the economic worth of Heshui RADIX PUERARIAE, important references is provided for the industrialization of Puerarin in Heshui RADIX PUERARIAE.
It should be appreciated that the application of the present invention is not limited to above-mentioned citing, for those of ordinary skills, can be improved according to the above description or convert, all these modifications and variations all should belong to the protection domain of claims of the present invention.
Claims (7)
1. Puerarin method of purification in a Heshui RADIX PUERARIAE, it is characterised in that described method is:
The Heshui RADIX PUERARIAE being cut into bulk is carried out forced air drying about 5-7h, and temperature is 60-80 DEG C, puts in flask after sample is moved to pulverize in mixer respectively, add ethanol solution, hot dipping 1-3h in the water-bath of 75-85 DEG C, then filter, collect filtrate, add ethanol solution in filter residue, at the same temperature, hot dipping 1-3h, filter, merge the filtrate of twice collection, the water-bath of 75-85 DEG C is evaporated ethanol, then put and be dried to constant weight to electric air drier.
The extracting method of Puerarin in Heshui RADIX PUERARIAE the most according to claim 1, it is characterised in that described Heshui RADIX PUERARIAE takes from root of kudzu vine skin.
The extracting method of Puerarin in Heshui RADIX PUERARIAE the most according to claim 1, it is characterised in that it is 6 h that described Heshui Pachyrhizua angulatus carries out the time of forced air drying, and dry temperature is 70 DEG C.
The extracting method of Puerarin in Heshui RADIX PUERARIAE the most according to claim 1, it is characterised in that during extraction, the solid-liquid ratio according to mass ratio 1:8 adds ethanol solution.
The extracting method of Puerarin in Heshui RADIX PUERARIAE the most according to claim 1, it is characterised in that the volume fraction of ethanol solution used is 60%.
The extracting method of Puerarin in Heshui RADIX PUERARIAE the most according to claim 1, it is characterised in that during extraction, the hot dipping time is 2h.
The extracting method of Puerarin in standing grain Heshui RADIX PUERARIAE the most according to claim 1, it is characterised in that the temperature that described hot dipping processes is all 80 DEG C with the temperature of the volatilization carrying out ethanol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610356902.8A CN105832813A (en) | 2016-05-26 | 2016-05-26 | Method for purifying puerarin in Heshui radix pueraria thomsonii |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610356902.8A CN105832813A (en) | 2016-05-26 | 2016-05-26 | Method for purifying puerarin in Heshui radix pueraria thomsonii |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105832813A true CN105832813A (en) | 2016-08-10 |
Family
ID=56594584
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610356902.8A Pending CN105832813A (en) | 2016-05-26 | 2016-05-26 | Method for purifying puerarin in Heshui radix pueraria thomsonii |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105832813A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110679799A (en) * | 2019-09-11 | 2020-01-14 | 南昌大学 | Extract capable of increasing intestinal mucus layer Muspiridae flora and composition thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101570581A (en) * | 2009-06-10 | 2009-11-04 | 华中农业大学 | Method for comprehensively extracting pueraria polysaccharide and flavone |
| CN102973643A (en) * | 2012-12-14 | 2013-03-20 | 安徽珠峰生物科技有限公司 | Extraction process of radix puerariae and measuring method of puerarin and total puerariae flavone |
-
2016
- 2016-05-26 CN CN201610356902.8A patent/CN105832813A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101570581A (en) * | 2009-06-10 | 2009-11-04 | 华中农业大学 | Method for comprehensively extracting pueraria polysaccharide and flavone |
| CN102973643A (en) * | 2012-12-14 | 2013-03-20 | 安徽珠峰生物科技有限公司 | Extraction process of radix puerariae and measuring method of puerarin and total puerariae flavone |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110679799A (en) * | 2019-09-11 | 2020-01-14 | 南昌大学 | Extract capable of increasing intestinal mucus layer Muspiridae flora and composition thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104304582A (en) | Cyclocarya paliurus tea and preparation method thereof | |
| CN104840501A (en) | Preparation method for total flavonoids of chrysanthemum | |
| CN103960396A (en) | Peony tea cream and preparation method thereof | |
| CN102533433A (en) | Method for supercritical extraction of sea buckthorn oil | |
| CN102206550B (en) | Method for preparing osmanthus concrete and osmanthus total flavonoid through enzyme hydrolysis technology | |
| CN109609279A (en) | A kind of highly effective extraction method of agilawood essential oil | |
| CN106916624A (en) | A kind of idesia polycarpa oil CO 2 supercritical extracting method | |
| CN104593441A (en) | Method for simultaneously extracting volatile oil, polysaccharides and amino acids from tricholoma matsutake | |
| CN101514219B (en) | Method for extracting black rice anthocyanins | |
| CN108014151A (en) | A kind of Angelica sinensis drying method | |
| CN104610394A (en) | Method for extracting phlorizin with different purity from twigs and green leaves of Malus hupehensis | |
| CN104558066A (en) | Extraction method for arbutin | |
| US20100316752A1 (en) | Method for extracing substances from soapberry fruit and seed and products made therefrom | |
| CN113633589A (en) | Extraction process of cherry blossom extract for cherry blossom moisturizing hair conditioner | |
| CN103446246A (en) | Process for extracting effective ingredients from myrtle roots | |
| CN105832813A (en) | Method for purifying puerarin in Heshui radix pueraria thomsonii | |
| CN109021043A (en) | A kind of extracting method of Paeoniflorin | |
| US20130130329A1 (en) | Method for extracting substances from soapberry fruit and its seeds | |
| CN101292670B (en) | Method for preparing plant jatropha curcas insecticide | |
| CN105532350B (en) | A kind of preparation method of hainan holly leaf ginkgo biloba extract compound tea | |
| CN104367622A (en) | Method for extracting alkaloids from herba leonuri | |
| CN105537248B (en) | The method of comprehensive utilization of banana stem leaves | |
| CN108164572A (en) | A kind of method using stem of noble dendrobium extraction tannic acid | |
| CN104277139A (en) | Method for extracting flammulina velutipes polysaccharide | |
| CN107669713A (en) | A kind of extraction process of ginko leaves flavone class compound |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160810 |