CN105831465A

CN105831465A - Application of lactobacillus plantarum strain in reduction on antibiotic residue in large-scale broiler chicken breeding

Info

Publication number: CN105831465A
Application number: CN201610181588.4A
Authority: CN
Inventors: 赵金山; 高鹏飞; 李和刚; 刘华伟; 杨培培
Original assignee: Qingdao Agricultural University
Current assignee: Qingdao Agricultural University
Priority date: 2016-03-25
Filing date: 2016-03-25
Publication date: 2016-08-10
Anticipated expiration: 2036-03-25
Also published as: CN105831465B

Abstract

The invention discloses an application of a lactobacillus plantarum strain in reduction on antibiotic residue in large-scale broiler chicken breeding and belongs to the field of feed-use microecologic agents. In the invention, a lactobacillus plantarum P-8 fermentation liquid is employed as a feed additive, and a result proves that the fermentation liquid effectively reduces the antibiotic residue in chicken meat during the large-scale broiler chicken breeding, thereby avoiding environment pollution and avoiding damage on people health due to the antibiotic residue in chicken. The application has important economic and social significances.

Description

One lactobacillus plantarum reduce extensive broiler breeding time antibiotic remains in Application

Technical field

The present invention relates to the Lactobacillus plantarum that a strain is isolatable from the traditional dairy products clabber of the Inner Mongol (Lactobacillus plantarum P-8) application during extensive broiler breeding, belongs to poultry feeding micro-ecological Formulation art.

Background technology

In the past few decades, antibiotic is applied in feed industry on a large scale as feed additive, in prevention Livestock and poultry, promotes growth of animal, improves price of deed aspect and has played indelible effect；Greatly improve poultry simultaneously The quantity of fowl and the yield of livestock products, the positive promotion development of domestic and international animal husbandry, create the most considerable economic effect Benefit.But along with the life-time service of antibiotic, drug residue problem not only causes huge pollution to environment, also can affect poultry The health of the mankind is caused serious harm by product quality.But, according to the present situation of current China aquaculture, people still do not have Finding preferable Substitutes For Antibiotic, a longer time period, antibiotic is still that livestock and poultry cultivation industry is requisite Disease-resistant rush increases medicament.Therefore, study and develop the Tiny ecosystem of antibiotic remains during a kind of effective reduction large-scale farming Preparation, this seems extremely important.

Lactobacillus plantarum (Lactobacillus plantarum) P-8, is isolatable from Inner Mongol traditional natural fermentation clabber In, there is the anti-gastrointestinal tract Digestive system tolerance of excellence, it is possible to survive in Intestine of Broiler；It is micro-that this bacterial strain has been preserved in China Biological inoculum preservation administration committee's common micro-organisms center, address is North Star West Road 1, Chaoyang District, city of BeiJing, China institute 3, The preservation time is on 06 28th, 2012, and preserving number is CGMCC No.6312.CN102994422A discloses Lactobacillus plantarum P- 8 application in improving alcoholic liver injury, and answering in leaven, fermented dairy product, health food, health food With.CN104630117A disclose feed Lactobacillus plantarum P-8 can improve total antibacterial in 42 age in days chicken intestinal, Lactobacillea, Streptococceae, Fusobacterium subspecies, bacillus bifidus race and Veillonella subspecies quantity；Escherichia can be significantly reduced simultaneously and take off Sulfur vibrio quantity, thus reach to improve the effect of intestinal microflora structure.CN104664080A discloses with plant breast Bacillus P-8 fermented bean cake carrys out Peru Fish Dietary, thus the antinutritional factor content caused after solving other protein sources Peru Fish Dietary The problems such as high, palatability poor, poor growth, infringement animal health.

By prior art carries out complete search analysis discovery, Lactobacillus plantarum P-8 can the most still not to have document to report To effectively facilitate metabolism or the excretion of antibiotic in Broiler Chicken.

Summary of the invention

In view of the deficiencies in the prior art, it is an object of the invention to provide a strain and can remarkably promote antibiotic generation in Broiler Chicken Thank or/and Lactobacillus plantarum (Lactobacillus plantarum) in draining, and this bacterial strain is at extensive broiler breeding During application.

The Lactobacillus plantarum P-8 (L.plantarum P-8) that the present invention provides is Agricultural University of the Inner Mongol's milk biology skill The excellent probiotic lactobacillus of strain that art and key lab of engineering education portion are isolatable from natural fermentation yogurt sample, this bacterial strain Advantage includes the Digestive system tolerance of excellence, improves intestinal microbial population, improves blood lipid metabolism, antioxidation, raising immunity of organism water Equality probiotic properties.And 270 broiler breeding experimental studies were completed in 2014, result shows, L.plantarum P-8 energy Enough survive in Intestine of Broiler, improve intestinal microbial population structure of community, reduce harmful bacteria adhesion, infect, field planting, promote probiotics Growth, strengthens Intestine defense function；Humoral immunization, cellular immunization and gut-associated lymphoid tissue's immunity to broiler can produce It is beneficial to the regulation effect of body's immunity state；The growth performance in broiler late growing stage stage can be significantly improved.

In order to realize the purpose of the present invention, the present inventor is by lot of experiments research persistent exploration, finally further Experimental study in find, this Lactobacillus plantarum P-8 fermentation liquid is as feed additive, and it is by promoting antibiotic in Broiler Chicken Metabolism, or/and excretion, can effectively reduce antibiotic residual in Carnis Gallus domesticus during extensive broiler breeding, promotes that poultry are supported Grow the sustainable development of industry, be with a wide range of applications in field of animal feed.

Based on the studies above achievement, further object is that offer this Lactobacillus plantarum (Lactobacillus Plantarum) P-8 multiple new application during broiler breeding, including:

Above-mentioned Lactobacillus plantarum P-8 is promoting antibiotic metabolism in Broiler Chicken or/and application in Pai Guo；

Above-mentioned Lactobacillus plantarum P-8 promotes antibiotic metabolism in Broiler Chicken or/and in the ecological agent of excretion in preparation Application；

Above-mentioned Lactobacillus plantarum P-8 is the application in antibiotic remains when reducing extensive broiler breeding.

It should be noted that Lactobacillus plantarum P-8 of the present invention reduces the application of antibiotic remains during broiler breeding In, described antibiotic is selected from following one or more: lincomycin hydrochloride, clindamycin, spectinomycin.Further preferably Described antibiotic is lincomycin hydrochloride.

Compared with prior art, Lactobacillus plantarum (Lactobacillus plantarum) the P-8 conduct that the present invention provides Additive, can effectively reduce antibiotic residual in Carnis Gallus domesticus during extensive broiler breeding, thus avoid making environment The pollution become, and avoid the harm that human health causes by antibiotic remains broiler, there is important economic implications and society Can meaning.

Accompanying drawing explanation

Fig. 1 is broiler daily ingestion of probiotic bacteria L.plantarum P-8 situation.

Detailed description of the invention

The foregoing of the present invention is described in further detail by form more by the following examples, but should this not managed Solving and be only limitted to below example for the scope of the above-mentioned theme of the present invention, all technology realized based on foregoing of the present invention are equal Belong to the scope of the present invention.It addition, the experimental technique in following embodiment, if no special instructions, it is conventional method.

During the embodiment 1:Lactobacillus plantarum P-8 extensive broiler breeding of reduction, antibiotic is chicken The effect test of residual in meat

Broiler (Arbor Acres, be called for short AA) is the most secondary increasingly tests to select 42 ages in days Chinese mugworts to pull out, 240,000 every batch (point 12 hen houses, 20,000 every).Breeding way is supported for net, and male and female raises together with (cock: hen=1:1), and cultivation density is 12 Only/m².Every batch of test broiler is randomly divided into four groups, often group 60,000；Matched group 1 feeds normal feedstuff, gives conventional drinking water； Matched group 2 feeds normal feedstuff, gives containing the Lactobacillus plantarum L.plantarum 102 (lactic acid of Boar taint material scatol Bacterium Study on degradation, Li Feng, thesis for the doctorate, 2011) drinking water；Two experimental grouies feed normal feedstuff, give containing planting simultaneously The drinking water of thing lactobacillus L.plantarum P-8；Experimental period is (depending on concrete marketing time is according to death rate, to connect for 37-43 days Continuous 3 days death rates reach 1%, then be judged as delivering for sale).Each group was added a certain amount of within 0-30 days cultivation phases in basal diet Lincomycin hydrochloride, cultivation to drug withdrawal during 30 age in days, withdrawal time more than 7 days, until delivering for sale.EXPERIMENTAL DESIGN is shown in Table 1, basis Daily ration composition is shown in Table 2.Fig. 1 is shown in amount and the change of the daily ingestion of Lactobacillus plantarum of broiler.

Packet situation tested by table 1

Note: cultivation is to drug withdrawal during 30 age in days, withdrawal time more than 7 days.

Table 2 basal diet composition and trophic level (air bells dry basis %)

Note: in daily ration, lincomycin hydrochloride addition is 1.5 or 3 g ton feedstuffs, cultivation, to drug withdrawal during 30 age in days, stops More than 7 days medicine phase.

After cultivation terminates, use " GB 29685-2013 national food safety standard: lincomycin in animal food, gram Woods mycin and the mensuration GC-MS of the many residuals of spectinomycin " measure lincomycin hydrochloride in chicken breast and chicken leg meat Residual quantity, the data obtained use SAS software one factor analysis of variance carry out significance analysis statistics.Testing result is shown in Table 3.

The table 3L.plantarum P-8 impact (n=450, x ± s) on Carnis Gallus domesticus antibiotic residual quantity

Note: different letters represent the diversity of the horizontal following data of p=0.01.

From testing result in table 3, compared with two matched groups, experimental group 1 and experimental group 2 can significantly reduce chicken The residual concentration (p < 0.01) of lincomycin hydrochloride in lower limb, pigeon chest, wherein in experimental group 1 Carnis Gallus domesticus, the residual of lincomycin hydrochloride is dense Degree slightly above experimental group 2, but between two groups, there was no significant difference (p > 0.05).I.e. during large-scale cultivation, at basal diet While middle interpolation lincomycin hydrochloride, daily ingestion of Lactobacillus plantarum L.plantarum P-8 fermentation liquid, meat can be significantly reduced Lincomycin hydrochloride residual concentration (p < 0.01) in chicken drumsticks, pigeon chest.

Claims

1. a lactobacillus plantarum is promoting antibiotic metabolism in Broiler Chicken or/and application in Pai Guo, described plant breast bar Bacterium is Lactobacillus plantarum (Lactobacillus plantarum) P-8, and its preserving number is CGMCC No.6312.

2. a lactobacillus plantarum promotes antibiotic metabolism in Broiler Chicken or/and application in the ecological agent of excretion in preparation, Described Lactobacillus plantarum is Lactobacillus plantarum (Lactobacillus plantarum) P-8, and its preserving number is CGMCC No.6312。

3. the lactobacillus plantarum application in antibiotic remains, described Lactobacillus plantarum when reducing extensive broiler breeding For Lactobacillus plantarum (Lactobacillus plantarum) P-8, its preserving number is CGMCC No.6312.

4. according to the application described in claim 1 or 2 or 3, it is characterised in that described antibiotic is selected from following one or many Kind: lincomycin hydrochloride, clindamycin, spectinomycin.

5. according to the application described in claim 1 or 2 or 3, it is characterised in that described antibiotic is lincomycin hydrochloride.