CN105831465B - Application of one lactobacillus plantarum when reducing extensive broiler breeding in antibiotic residue - Google Patents
Application of one lactobacillus plantarum when reducing extensive broiler breeding in antibiotic residue Download PDFInfo
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- CN105831465B CN105831465B CN201610181588.4A CN201610181588A CN105831465B CN 105831465 B CN105831465 B CN 105831465B CN 201610181588 A CN201610181588 A CN 201610181588A CN 105831465 B CN105831465 B CN 105831465B
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- lactobacillus plantarum
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Abstract
The invention discloses application of the lactobacillus plantarum when reducing extensive broiler breeding in antibiotic residue, belong to livestock and poultry feeding micro-ecological preparation field.The present invention is using lactobacillus plantarum P-8 fermentation liquid as feed addictive, as a result, it has been found that residual of the antibiotic in chicken during extensive broiler breeding can be effectively reduced in it, to avoid pollution on the environment, and the harm caused by human health of antibiotic residue broiler chicken is avoided, there is important economic significance and social effect.
Description
Technical field
The present invention relates to one plant to be isolated from Inner Mongol traditional dairy products --- the lactobacillus plantarum in yoghurt
The application of (Lactobacillus plantarum P-8) during extensive broiler breeding, belongs to livestock and poultry feeding micro-ecological
Formulation art.
Background technique
In the past few decades, antibiotic is applied in feed industry on a large scale as feed addictive, is being prevented
Livestock and poultry promotes growth of animal, has played indelible effect in terms of improving the price of deed；Poultry is greatly improved simultaneously
The quantity of fowl and the yield of livestock products, the positive promotion development of domestic and international animal husbandry create more considerable economic effect
Benefit.But with the long-time service of antibiotic, medicament residue problem not only causes huge pollution to environment, also will affect livestock and poultry
Product quality causes serious harm to the health of the mankind.However, according to the status of current aquaculture industry of China, people do not have still
Ideal Substitutes For Antibiotic is found, in a longer time section, antibiotic is still that livestock and poultry cultivation industry is essential
Disease-resistant rush increases medicament.Therefore, it studies and develops a kind of Tiny ecosystem that antibiotic residue during large-scale farming is effectively reduced
Preparation, this seems extremely important.
Lactobacillus plantarum (Lactobacillus plantarum) P-8 is isolated from Inner Mongol traditional natural fermentation yoghurt
In, there is excellent anti-gastrointestinal tract digestive juice tolerance, can survive in Intestine of Broiler；It is micro- that the bacterial strain has been preserved in China
Biological inoculum preservation administration committee common micro-organisms center, address are city, BeiJing, China, North Star West Road 1, Chaoyang District institute 3,
The preservation time is on 06 28th, 2012, and deposit number is CGMCC No.6312.CN102994422A discloses lactobacillus plantarum P-
8 are improving the application in alcoholic liver injury, and answering in leavening, fermented dairy product, healthy food, health food
With.CN104630117A disclose feeding lactobacillus plantarum P-8 can be improved total bacterium in 42 age in days chicken intestinals, Lactobacillea,
Streptococceae, Fusobacterium subspecies, Bifidobacterium race and Veillonella subspecies quantity；Escherichia can be significantly reduced simultaneously and is taken off
Sulphur vibrio quantity, to achieve the effect that improve intestinal microflora structure.CN104664080A is disclosed with plant cream
Bacillus P-8 fermented bean dregs substitute fish meal, thus the anti-nutritional factors content caused by after solving other protein sources substitution fish meal
Height, palatability are poor, slow growth, damage the problems such as animal health.
It is found by carrying out complete search analysis to the prior art, it at present still can without document report lactobacillus plantarum P-8
To effectively facilitate the metabolism or excretion of antibiotic in Broiler Chicken.
Summary of the invention
In view of the deficiencies in the prior art, the purpose of the present invention is to provide one plant can significantly promote antibiotic generation in Broiler Chicken
Lactobacillus plantarum (Lactobacillus plantarum) and the bacterial strain in thanking or/and draining is in extensive broiler breeding
Application in the process.
Lactobacillus plantarum P-8 (L.plantarum P-8) provided by the invention is Agricultural University of the Inner Mongol's dairy products biology skill
Art and key lab, engineering education portion are isolated from one plant of excellent probiotic lactobacillus in spontaneous fermentation yogurt sample, the bacterial strain
Advantage includes excellent digestive juice tolerance, improves intestinal flora, improve blood lipid metabolism, is anti-oxidant, improving immunity of organism water
Equality probiotic properties.And 270 broiler breeding experimental studies were completed in 2014, the results showed that, L.plantarum P-8 energy
It enough survives in Intestine of Broiler, improves intestinal flora structure of community, reduce the adherency of harmful bacteria, infect, be colonized, promote beneficial bacterium
Growth enhances Intestine defense function；It is immune to the humoral immunity, cellular immunity and gut-associated lymphoid tissue of broiler chicken to have can produce
Conducive to the adjustment effect of body's immunity state；The growth performance in broiler chicken late growth stage can be significantly improved.
In order to achieve the object of the present invention, the present inventor is by a large number of experiments research and persistent exploration, finally further
Experimental study in find, lactobacillus plantarum P-8 fermentation liquid is as feed addictive, by promoting antibiotic in Broiler Chicken
Metabolism or/and excretion, can be effectively reduced residual of the antibiotic in chicken during extensive broiler breeding, and livestock and poultry is promoted to support
The sustainable development for growing industry is with a wide range of applications in field of animal feed.
Based on the studies above achievement, it is another object of the present invention to provide the lactobacillus plantarum (Lactobacillus
Plantarum) a variety of new applications of P-8 during broiler breeding, comprising:
Application of the above-mentioned lactobacillus plantarum P-8 in promoting antibiotic metabolism in Broiler Chicken or/and draining；
Above-mentioned lactobacillus plantarum P-8 promotes in Broiler Chicken in the ecological agent of antibiotic metabolism or/and excretion in preparation
Application；
Application of the above-mentioned lactobacillus plantarum P-8 when reducing extensive broiler breeding in antibiotic residue.
It should be noted that lactobacillus plantarum P-8 of the present invention reduces the application of antibiotic residue during broiler breeding
In, the antibiotic is chosen from the followings one or more: Lincomycin Hydrochloride, clindamycin, spectinomycin.Further preferably
The antibiotic is Lincomycin Hydrochloride.
Compared with prior art, lactobacillus plantarum (Lactobacillus plantarum) P-8 conduct provided by the invention
Residual of the antibiotic in chicken during extensive broiler breeding can be effectively reduced, to avoid making environment in additive
At pollution, and avoid the harm caused by human health of antibiotic residue broiler chicken, there is important economic significance and society
It can meaning.
Detailed description of the invention
Fig. 1 is the daily ingestion of probiotics L.plantarum P-8 situation of broiler chicken.
Specific embodiment
Form is described in further detail above content of the invention again by the following examples, but should not manage this
For solution for the scope of the above subject matter of the present invention is limited to the following embodiments, all technologies realized based on above content of the present invention are equal
Belong to the scope of the present invention.In addition, the experimental method in following embodiments is unless otherwise instructed conventional method.
Antibiotic is in chicken during embodiment 1:Lactobacillus plantarum P-8 reduces extensive broiler breeding
Remaining effect test in meat
Selecting 42 ages in days Chinese mugwort to pull out, increasingly broiler chicken (Arbor Acres, abbreviation AA) is secondary in three batches is tested, and every batch of 240,000
(point 12 henhouses, 20,000 every).Breeding way is that net is supported, and male and female raises together (cock: hen=1:1), cultivation density 12
Only/m2.Every batch of test broiler chicken is randomly divided into four groups, every group 60,000；Control group 1 feeds basal feed, gives conventional drinking water；
Control group 2 feeds basal feed, gives (the lactic acid of Boar taint substance scatol of L.plantarum containing lactobacillus plantarum 102
Bacterium Study on degradation, Li Feng, doctoral thesis, 2011) drinking water；Two experimental groups feed basal feed, while giving containing plant
The drinking water of object lactobacillus L.plantarum P-8；Experimental period is that (specific marketing time connected depending on death and culling rate in 37-43 days
Continuous 3 days death and culling rates reach 1%, then being judged as can deliver for sale).Each group was added a certain amount of within 0-30 days cultivation phases in basal diet
Lincomycin Hydrochloride, be discontinued when cultivation is to 30 age in days, withdrawal time 7 days or more, until delivering for sale.Experimental design is shown in Table 1, basis
Diet group is at being shown in Table 2.Fig. 1 is shown in the amount of the daily ingestion of lactobacillus plantarum of broiler chicken and variation.
The test grouping situation of table 1
Note: being discontinued when cultivation to 30 age in days, and withdrawal time 7 days or more.
2 basal diet of table composition and trophic level (air bells dry basis %)
Note: Lincomycin Hydrochloride additive amount is 1.5 or 3 g tons of feeds in daily ration, is discontinued, stops when cultivation is to 30 age in days
The medicine phase 7 days or more.
After cultivation, using " GB 29685-2013 national food safety standard: lincomycin in animal food, gram
Woods mycin and the how remaining measurement gas-chromatography-mass spectrography of spectinomycin " measures Lincomycin Hydrochloride in Fresh Grade Breast and chicken leg meat
Residual quantity, the data obtained using SAS software one-way analysis of variance carry out significance analysis statistics.Testing result is shown in Table 3.
Influence (n=450, x ± s) of the table 3L.plantarum P-8 to chicken antibiotic residual quantity
Note: different letters represent the otherness of the horizontal following data of p=0.01
By testing result in table 3 it is found that compared with two control groups, experimental group 1 and experimental group 2 can significantly reduce chicken
The residual concentration (p < 0.01) of Lincomycin Hydrochloride in leg, pigeon breast, wherein the residual of Lincomycin Hydrochloride is dense in 1 chicken of experimental group
Degree is slightly above experimental group 2, but there was no significant difference between two groups (p > 0.05).I.e. during large-scale cultivation, in basal diet
While middle addition Lincomycin Hydrochloride, daily ingestion of lactobacillus plantarum L.plantarum P-8 fermentation liquid can significantly reduce meat
Lincomycin Hydrochloride residual concentration (p < 0.01) in chicken chicken leg, pigeon breast.
Claims (3)
1. application of the lactobacillus plantarum in promoting antibiotic metabolism in Broiler Chicken or/and draining, the plant cream bar
Bacterium is lactobacillus plantarum (Lactobacillus plantarum) P-8, and deposit number is CGMCC No. 6312, and described is anti-
Raw element one or two chosen from the followings: Lincomycin Hydrochloride, clindamycin.
2. a lactobacillus plantarum promotes the application in Broiler Chicken in the ecological agent of antibiotic metabolism or/and excretion in preparation,
The lactobacillus plantarum is lactobacillus plantarum (Lactobacillus plantarum) P-8, and deposit number is CGMCC No.
6312, antibiotic one or two chosen from the followings: Lincomycin Hydrochloride, clindamycin.
3. application of the lactobacillus plantarum when reducing extensive broiler breeding in antibiotic residue, the lactobacillus plantarum
For lactobacillus plantarum (Lactobacillus plantarum) P-8, deposit number is CGMCC No. 6312, the antibiosis
Element one or two chosen from the followings: Lincomycin Hydrochloride, clindamycin.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610181588.4A CN105831465B (en) | 2016-03-25 | 2016-03-25 | Application of one lactobacillus plantarum when reducing extensive broiler breeding in antibiotic residue |
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| CN201610181588.4A CN105831465B (en) | 2016-03-25 | 2016-03-25 | Application of one lactobacillus plantarum when reducing extensive broiler breeding in antibiotic residue |
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| CN110200135A (en) * | 2019-05-31 | 2019-09-06 | 青岛农业大学 | It is a kind of alleviate broiler chicken heat stress lactic acid bacteria bacterium powder and its application |
| CN115381005B (en) * | 2022-08-25 | 2023-09-19 | 中国农业科学院饲料研究所 | Application of Lactobacillus plantarum CICC 6240 in reducing antibiotic residues in aquatic products |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550850A (en) * | 2010-12-31 | 2012-07-11 | 朱帆 | Biological nanometer feed composition and application thereof |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550850A (en) * | 2010-12-31 | 2012-07-11 | 朱帆 | Biological nanometer feed composition and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| Effects of the addition of probiotic containing both bacteria and yeast or an antibiotic on performance parameters, mortality rate and antibiotic residue in broilers;Thongsong B，et.al;《The Thai Journal of Veterinary Medicine》;20081231;第38卷(第1期);第17-26页 * |
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