CN105816495A - Traditional Chinese medicine compound extract product and application thereof - Google Patents
Traditional Chinese medicine compound extract product and application thereof Download PDFInfo
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- CN105816495A CN105816495A CN201510007282.2A CN201510007282A CN105816495A CN 105816495 A CN105816495 A CN 105816495A CN 201510007282 A CN201510007282 A CN 201510007282A CN 105816495 A CN105816495 A CN 105816495A
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Abstract
The invention discloses a traditional Chinese medicine compound extract product. The compound extract product is prepared by crushing ginseng, pseudo-ginseng and saposhnikovia divaricata according to a ratio of 1-10:1-10:1-10 and mixing the above materials, and then extracting a mixture by using 75-95% of ethanol. The invention also discloses an application of the compound extract product. The traditional Chinese medicine compound extract product has good oxidative damage protection activity and promotion of nerve cell axons and axon growth activity, and is suitable for preventing and treating neurodegenerative disease.
Description
Technical field
The present invention relates to Chinese medicine extract, particularly relate to a kind of Radix Ginseng, Radix Notoginseng, Radix Saposhnikoviae Chinese medicinal compound extract and application thereof.
Background technology
Neurodegenerative diseases is a series of sacred diseases being characterized with Neuron Apoptosis, and it shows as progressive dementia clinically, intelligence is extensively degenerated, health is out of hand.Because the most impaired being just difficult to of neuron returns to health status, so the treatment of degenerative disease always one of medical treatment difficult point, the prevention simultaneously for neurodegenerative diseases is particularly important.
Panaxynol (falcarinol or panaxynol) and panaxydol (panaxydol) belong to falcarinol type alkynol, distribution is had in plurality of Chinese and food plant (Radix Ginseng, Radix Notoginseng, Radix Saposhnikoviae, Radix Dauci Sativae, parsnip, parsley etc.), wherein Radix Ginseng mainly contains Panaxynol, Radix Notoginseng mainly contains panaxydol, and Radix Saposhnikoviae mainly contains Panaxynol and sickle celery glycol (falcarindiol).Owing to falcarinol type alkynol monomer is to light, thermally labile, therefore any of which compound is manufactured separately into medicine and all will face the difficulty keeping medicine stability.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of Radix Ginseng, Radix Notoginseng, Radix Saposhnikoviae Chinese medicinal compound extract; its active component contained such as falcarinol type alkynol can exist in this Chinese medicinal compound extract steadily in the long term, and this compound extract has good neurotrophy and protective effect.
In addition, it is also desirable to provide the preparation method of a kind of Chinese medicinal compound extract, and the application that this compound extract is in the medicine of preparation prevention and/or treatment neurodegenerative diseases.
In order to solve above-mentioned technical problem, the present invention is achieved through the following technical solutions:
In one aspect of the invention, it is provided that a kind of Chinese medicinal compound extract, this compound extract be Radix Ginseng, Radix Notoginseng, Radix Saposhnikoviae are respectively pulverized after by 1～10:1～10:1～10 proportionings mixing, prepare with 75-95% ethanol extraction.
Preferably, described Radix Ginseng, Radix Notoginseng, the proportioning of Radix Saposhnikoviae are 1:4.5:4.5.
Preferably, described Radix Ginseng, Radix Notoginseng, the proportioning of Radix Saposhnikoviae are 1:9.6:9.6.
Preferably, described Radix Ginseng, Radix Notoginseng, the proportioning of Radix Saposhnikoviae are 4.5:4.5:1.
Preferably, described Radix Ginseng, Radix Notoginseng, the proportioning of Radix Saposhnikoviae are 9.5:1:4.75.
In another aspect of this invention, it is provided that the preparation method of a kind of Chinese medicinal compound extract, comprise the following steps:
By 1～10:1～10:1～10 proportioning mixing after Radix Ginseng, Radix Notoginseng, Radix Saposhnikoviae are respectively pulverized, use 75-95% ethanol extraction, obtain compound extract.
Preferably, with three kinds of Chinese medicine compound powder 15～20 times of 95% alcohol at normal temperature extraction.
Preferably, after with 95% ethanol extraction, still further comprise step: decompression and solvent recovery, obtain compound extract.
In another aspect of this invention, a kind of compound Chinese medicinal preparation comprising above-mentioned Chinese medicinal compound extract is additionally provided.
In another aspect of this invention, a kind of prevention comprising above-mentioned Chinese medicinal compound extract and/or the medicine for the treatment of neurodegenerative diseases are additionally provided.Described neurodegenerative diseases includes: parkinson disease, Alzheimer, hungtington's chorea, amyotrophic lateral sclerosis, cerebral atrophy etc..
In another aspect of this invention, a kind of neurotrophy health product comprising above-mentioned Chinese medicinal compound extract are additionally provided.
In another aspect of this invention, the application in the medicine of preparation prevention and/or treatment neurodegenerative diseases of a kind of above-mentioned Chinese medicinal compound extract is additionally provided.
In another aspect of this invention, the application in preparation neurotrophy health product of a kind of above-mentioned Chinese medicinal compound extract is additionally provided.
The Chinese medicinal compound extract of the present invention, the activity that there is significantly rush cell axonal growth through promoting the experiment of axon growth active testing to show.Tetrazolium (Methyl-Thiazol-Tetrozolium, MTT) reducing process detects Chinese medicinal compound extract of the present invention to H2O2The protection situation of the PC12 cell of damage, result shows that this compound extract is to H2O2The PC12 cell of damage has significant protective effect, points out extract of the present invention to have the function of protection neural cell injury, it is adaptable to the treatment and prevention of neurodegenerative diseases.
Accompanying drawing explanation
The present invention is further detailed explanation with detailed description of the invention below in conjunction with the accompanying drawings.
Fig. 1 is that the embodiment of the present invention 2 compound extract promotees PC12 cell axonal growth figure (icon scale is 50 μMs)；
Fig. 2 is the enlarged drawing that the embodiment of the present invention 2 compound extract PSP-1, PSP-2, PSP-3 promote PC12 cell axonal growth；
Fig. 3 is that the embodiment of the present invention 3 compound extract is to H2O2The PC12 cell of damage has the result figure of protection activity.
Detailed description of the invention
The compound extract of the present invention, with the small-molecule substance in Radix Ginseng, Radix Notoginseng, Radix Saposhnikoviae three taste Chinese medicine as active component, by mixing after above-mentioned pulverizing medicinal materials, prepares with 95% ethanol extraction.Through test cell line confirm, this compound extract not only have well promote nerve cell axons, axonal growth activity, but also have significant oxidative damage protection activity, be suitable to prevention and the treatment of neurodegenerative diseases.
Embodiment 1 Radix Ginseng, Radix Notoginseng, the preparation of Radix Saposhnikoviae Chinese medicinal compound extract
Radix Ginseng, Radix Notoginseng, three kinds of Chinese crude drugs of Radix Saposhnikoviae are pulverized respectively and made powder, then the proportioning composition medical material mixed-powder 6g of according to the form below 1, add 75-95% ethanol 100mL room temperature and extract 24 hours, then decompression and solvent recovery, obtain compound extract.
The proportioning of 1 three kinds of medical materials of table
Embodiment 2 compound extract promotees the test of nerve cell axons growth activity
1. experimental technique
1.1 cells are cultivated and are processed
Undifferentiated PC12 cell is purchased from Chinese Shanghai life science institute cell bank, at 37 DEG C, 5%CO2Under the conditions of cultivate, culture medium is containing 10% horse serum, 5% hyclone, the DMEM high glucose medium of 1% glutamine, changes liquid every other day.Culture bottle and 24 orifice plates are coated for 24 hours in advance with poly-D-lysine.Need to add the compound extract of desired concn after one day or positive control medicine is hatched together at bed board according to experiment, change liquid every other day and rejoin compound extract and the positive control medicine of desired concn.Experiment is all provided with Normal group, compound extract process group and positive controls (NGF) every time.
1.2 results are taken pictures
After 7 days, under inverted microscope, every hole randomly selects 5 visuals field and takes pictures.
2. experimental result
2.1 compound extracts promote PC12 cell axonal growth photographic result
As can be seen from Figure 1, the compound extract of the present invention can promote PC12 cell axonal growth, Fig. 2 is the enlarged drawing that table 1 compound recipe PSP-1, PSP-2, PSP-3 promote PC12 cell axonal growth, can more clearly show that compound extract promotees the activity of PC12 cell axonal growth.
2.2 compound extracts promote PC12 cell axonal growth statistical result
Using ImageJ image analysis system to measure synapse cell length and aixs cylinder number in taken the photograph photo, calculate average synapse quantity and length, result is as shown in table 2.
The average aixs cylinder number of table 2 and average axon length statistical table
Understood PSP-1, PSP-2 and PSP-3 by statistical result and all can promote PC12 axon growth by significance.
3. conclusion
The compound extract of the present invention has the activity promoting PC12 cell axonal growth, and especially PSP-1 extract has the activity being obviously promoted PC12 cell axonal growth.Compound extract of the present invention is pointed out to be likely to be of the function promoting nerve cell axons growth.
The oxidative damage protection active testing of embodiment 3 compound extract
Compound extract of the present invention is detected to H by tetrazolium (Methyl-Thiazol-Tetrozolium, MTT) reducing process2O2The protection situation of the PC12 cell of damage.
1. cell is cultivated
Differentiated PC12 cell is purchased from Chinese Shanghai life science institute cell bank, at 37 DEG C, 5%CO2Under the conditions of cultivate, culture medium is containing 10% hyclone, the DMEM high glucose medium of 1% glutamine, changes liquid every other day.Within after plating cells second day, change liquid, add after compound extract is hatched 6 hours altogether and add H2O2So that it is concentration is 200 μMs.
2.MTT colorimetric
Take the logarithm the cell counting of trophophase, adjust cell density to 2 × 103/ mL, sets dosing, normal (the most only add cell and be not added with medicine), damage (only add H in 96 orifice plates2O2It is not added with medicine) and blank (the most only adding cell free broth) four groups.The every hole of dosing group is separately added into 200 μ L cell suspension and compound extract that 5 μ L concentration are 2mg/mL makes the final concentration of 50 μ g/mL of extract, and each concentration sets three parallel holes, adds compound extract and H2O2Rear cultivation 72h, abandons culture fluid afterwards, and every hole adds MTT solution (5mg/mL) 20 μ L, and after continuing to hatch 4h, every hole adds 150 μ LDMSO, microplate reader detection light absorption value after concussion 10min.Measure wavelength 570nm, reference wavelength 655nm.Cell survival rate is calculated by formula below.
Cell survival rate (%)=(dosing-blank)/(comparison-blank) × 100
3. result
As it is shown on figure 3, compound extract is to H2O2Damage PC12 cell has protective effect, and wherein PSP-2 extract has notable protective effect, and PSP-4 extract has preferable protective effect.In Fig. 3, " * " refers to p < 0.1, and " * * " refers to p < 0.01.
4. conclusion
The compound extract of the present invention is to H2O2The PC12 cell of damage has protection activity, points out it to have the function of protection neural cell injury.
Embodiment described above only have expressed embodiments of the present invention, and it describes more concrete and detailed, but therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that, for the person of ordinary skill of the art, without departing from the inventive concept of the premise, it is also possible to make some deformation and improvement, these broadly fall into protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (10)
1. a Chinese medicinal compound extract, it is characterised in that this compound extract be Radix Ginseng, Radix Notoginseng, Radix Saposhnikoviae are respectively pulverized after by 1～10:1～10:1～10 proportionings mixing, with 75-95% ethanol extraction prepare.
Chinese medicinal compound extract the most according to claim 1, it is characterised in that described Radix Ginseng, Radix Notoginseng, the proportioning of Radix Saposhnikoviae are 1:4.5:4.5.
Chinese medicinal compound extract the most according to claim 1, it is characterised in that described Radix Ginseng, Radix Notoginseng, the proportioning of Radix Saposhnikoviae are 1:9.6:9.6.
Chinese medicinal compound extract the most according to claim 1, it is characterised in that described Radix Ginseng, Radix Notoginseng, the proportioning of Radix Saposhnikoviae are 4.5:4.5:1.
Chinese medicinal compound extract the most according to claim 1, it is characterised in that described Radix Ginseng, Radix Notoginseng, the proportioning of Radix Saposhnikoviae are 9.5:1:4.75.
6. a compound Chinese medicinal preparation, it is characterised in that comprise the Chinese medicinal compound extract described in any one of claim 1-5.
7. a prevention and/or the medicine for the treatment of neurodegenerative diseases, it is characterised in that comprise the Chinese medicinal compound extract described in any one of claim 1-5.
8. neurotrophy health product, it is characterised in that comprise the Chinese medicinal compound extract described in any one of claim 1-5.
9. the application in the medicine of preparation prevention and/or treatment neurodegenerative diseases of the Chinese medicinal compound extract described in any one of claim 1-5.
10. the application in preparation neurotrophy health product of the Chinese medicinal compound extract described in any one of claim 1-5.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510007282.2A CN105816495A (en) | 2015-01-07 | 2015-01-07 | Traditional Chinese medicine compound extract product and application thereof |
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| CN201510007282.2A CN105816495A (en) | 2015-01-07 | 2015-01-07 | Traditional Chinese medicine compound extract product and application thereof |
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| CN105816495A true CN105816495A (en) | 2016-08-03 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040069413A (en) * | 2003-01-29 | 2004-08-06 | 김호철 | Pharmaceutical composition comprising the falcarindiol derivatives having neuro-protective activity |
| CN101254194A (en) * | 2008-03-17 | 2008-09-03 | 沈阳药科大学 | Applications of panax saponin-Re in neurocyte protection |
| CN101502547A (en) * | 2009-03-11 | 2009-08-12 | 昆明理工大学 | Application of notoginseng extract in preparing medicament for preventing and treating parkinsonism |
| KR20100134463A (en) * | 2009-06-15 | 2010-12-23 | 주식회사 엔에이치한삼인 | A lipid-soluble ginseng extract composition for the growth inhibition of cancer cell, and the medical composition and the health foods thereof |
| CN102018716A (en) * | 2009-09-14 | 2011-04-20 | 王泽君 | Medical application of protopanaxatriol and protopanaxadiol in nervous system diseases |
-
2015
- 2015-01-07 CN CN201510007282.2A patent/CN105816495A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040069413A (en) * | 2003-01-29 | 2004-08-06 | 김호철 | Pharmaceutical composition comprising the falcarindiol derivatives having neuro-protective activity |
| CN101254194A (en) * | 2008-03-17 | 2008-09-03 | 沈阳药科大学 | Applications of panax saponin-Re in neurocyte protection |
| CN101502547A (en) * | 2009-03-11 | 2009-08-12 | 昆明理工大学 | Application of notoginseng extract in preparing medicament for preventing and treating parkinsonism |
| KR20100134463A (en) * | 2009-06-15 | 2010-12-23 | 주식회사 엔에이치한삼인 | A lipid-soluble ginseng extract composition for the growth inhibition of cancer cell, and the medical composition and the health foods thereof |
| CN102018716A (en) * | 2009-09-14 | 2011-04-20 | 王泽君 | Medical application of protopanaxatriol and protopanaxadiol in nervous system diseases |
Non-Patent Citations (4)
| Title |
|---|
| BAO-MING NIE等: "Panaxydol and panaxynol protect cultured cortical neurons against A beta 25-35-induced toxicity", 《NEUROPHARMACOLOGY》 * |
| ZE-JIAN WANG等: "Panaxynol induces neurite outgrowth in PC12D cells via cAMP- and MAP kinase-dependent mechanisms", 《CHEMICO-BIOLOGICAL INTERACTIONS》 * |
| 王泽剑等: "人参炔醇对神经细胞的营养和保护作用", 《中国药学杂志》 * |
| 蔡锦贤等: "天然聚乙炔醇的研究进展", 《中草药》 * |
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