CN105795351A

CN105795351A - Probiotic and wheat malt composite flour and preparation method thereof

Info

Publication number: CN105795351A
Application number: CN201610204501.0A
Authority: CN
Inventors: 邵素英
Original assignee: Yiwu Jinyu Information Technology Co Ltd
Current assignee: Yiwu Jinyu Information Technology Co Ltd
Priority date: 2016-04-01
Filing date: 2016-04-01
Publication date: 2016-07-27

Abstract

The invention discloses probiotic and wheat malt composite flour and a preparation method thereof. Wheat malt flour with a natural color, flavor and taste is scientifically compounded with functional products such as lactobacillus plantarum powder, modified dietary fibers, enzyme powder, oligosaccharide and the like, the nutrition, the food safety and the health-care function of the wheat malt composite flour are improved, the probiotic performance and the quality stability of the wheat malt composite flour are significantly improved, components are simple, the color, the flavor and the taste are natural, the nutritional value is high, the health-care function is high, the content of bioactive substances is high, the composite flour contains a large quantity of viable probiotics and has high probiotic performance, functional metabolites of the probiotics are in a wide variety and high in content, the food safety is high, and the guarantee period is long. Ultrasonic cleaning, microwave instant puffing for embryo killing, high-voltage pulse, biological enzymolysis, soaking and germination promotion are adopted in the preparation process of the wheat malt, the soaking and germination time is short, the germination rate is high, the content of bioactive substances is high, the production efficiency is high, no environmental pollution is caused, and the preparation method is environment-friendly.

Description

A kind of probiotic bacteria Fructus Tritici aestivi composite powder and preparation method thereof

Technical field

The present invention relates to Semen Tritici aestivi deep processed product, be specifically related to a kind of probiotic bacteria Fructus Tritici aestivi composite powder and preparation method thereof.

Background technology

Wheat Sprout Powder be Fructus Tritici aestivi through ultralow temperature dried, low temperature particle abrasion technology, whole Fructus Tritici aestivi is ground to form superfine powder.Its character is gentle, is very easily absorbed by the body, and absorbance is up to more than 96%.It it is the green organic food with full nutrition of whole world original creation.The nutrition that human body is necessary can be provided, help body metabolism, discharge various toxin, make us reduce the danger of disease.Main component and effect 1. chlorophylls: the green chlorophyllous composition of blood is similar to the haemachrome of human body, can quickly dissolve in erythrocyte, make people rejuvenate.Chlorophyll also can enter in tissue, removes medicine and the toxin of residual, neutralizes the chemical substance being unfavorable for health, has liver heat removing, invigorates blood circulation, heart tonifying is dirty, the effect of blood sugar lowering, accelerating wound healing.2. abundant vitamin: Fructus Tritici aestivi contains abundant vitamin A, B, C, E.Vitamin A is wanted physical growth is fairly heavy, and can promote vision.Scientific circles are it has proven convenient that it is a kind of anticancer vitamin.Vitamin B is of value to nervous system, can prevention foot disorder of QI, promote appetite, allaying tiredness, helper cell picked-up carbohydrate energy.Vitamin C is extremely important to skin, tooth, eyes, muscle etc..Vitamin E is the cleaning agent of human body, can cardioprotection, slow down aging, be conducive to improving the symptoms such as cutis laxa, xerosis cutis, acne, anemia.3. the treasure-house of mineral: containing mineral such as calcium, ferrum, manganese, phosphorus, sodium, cobalt, zinc in Fructus Tritici aestivi.What is more important potassium ion, to improving constipation and dyspepsia, promotes intestinal peristalsis promoting and absorption function, keeps a lot of aspects such as the cardiac muscle strength with whole-body muscle, plays obvious action.4. the king of basic food: owing to containing substantial amounts of metallic ore material, the Herba Spinaciae (39.6) Gao Jinyi times (66.4) that degree of alkalinity is higher than vegetable neutral and alkali in Fructus Tritici aestivi, be the optimum food correcting acidic physique.5. ferment ferment is also called " enzyme ", is a kind of reactive protein.Ferment wide application, in each tissue of human body and organ, is the biological activity catalyst of needed by human, promotes that food decomposes and metabolism, promotes tissue repair, be the important active substances of human body biochemical reaction.It can be said that there is no life without ferment.About the general knowledge American physician DrAnnWigmore of Fructus Tritici aestivi because self suffering from cancer, and further investigate the function of Fructus Tritici aestivi, thus being found that and utilizing Fructus Tritici aestivi controlling the method for cancerous cell diffusion and be rescued.Also one has therefore been set up exclusively with Fructus Tritici aestivi to cure the medical centre of cancer.Scientist's years of researches through a lot of countries, it was demonstrated that Fructus Tritici aestivi can effectively adjust human metabolism and hormone secretion, can adjust various physical signs and tend to normal, comply fully with the healthy rule of nature, original, origin.Ancient Chinese famous medicine scholar's Li Shizhen (1518-1593 A.D.) so describes Fructus Tritici aestivi (wheat seedling) in Compendium of Material Medica: " acrid in the mouth, cold, nontoxic, cure mainly the alcoholism that disappears, sudden high fever, jaundice due to immoderate drinking, icteric sclera.And mash strand juice day drink it, solve again diseases due to noxious agents produced by various parasites；Liquor filter clothes, relieving restlessness is vexed, and during solution, disease is fanatic, move back chest and diaphragm heat, profit small intestinal." Fructus Tritici aestivi described in Compendium of Material Medica is not comprise root.From the angle of the traditional Chinese medical science, Fructus Tritici aestivi leaf belongs to cold, and rhizome belongs to scorching, so, the Fructus Tritici aestivi product of general commercial type is only with foliage portion, thus cold too high, it is easy to cause vomiting, dizzy side effect.EasyPha-max studies discovery, only the root of Fructus Tritici aestivi oneself, it is possible to offset the cold of its leaf.Therefore, the reservation of Fructus Tritici aestivi root is processed by EasyPha-max together, becomes a kind of not dry not cold nourishing healthy good merchantable brand.From the angle of science, the rhizome of Fructus Tritici aestivi contains abundant enzyme isoreactivity nutritional labeling.Experiment it turned out, and rhizome and leaf comprise multiple nutritional components respectively, coexists in proper proportions and constitutes complete nutrition entirety, and they make the absorption of nutrition be more prone to.The active component, particularly auxin found in Fructus Tritici aestivi rhizome, it is possible to promote the reparation of damaged cell.

At present, the more of pure Wheat Sprout Powder is prepared with Semen Tritici aestivi for raw material:

Chinese patent CN104146218A the invention discloses the Semen Tritici aestivi rich in selenium bud powder of a kind of new model, belongs to plant and field of food.Wheat seed is soaked in the sodium selenite aqueous solution of 200ppm 12-24 hour, then it is laid on the cultivation dish of a large amount of hole, side keep certain height without hydrospace, germinate at 20 DEG C of-30 DEG C of temperature, the sodium selenite aqueous solution of every 1 hours 200ppm sprays once, through 3-4 days time, when the height of Fructus Tritici aestivi is 2-3cm, the blade and the roots that grow germination are cut, and abandon the remaining part including seed.The blade cut and root are positioned below at the temperature of 70 DEG C and dry, then grinds.The raw material making Semen Tritici aestivi rich in selenium bud powder does not include seed, it is possible to reduce because seed goes mouldy the food safety risk brought.Organic selenium content in wheat root is higher than blade, is conducive to improving the content of organic selenium in whole product.Patent disclosed above is soaked and germinating time is long, and efficiency is low, and cost is high, and soak easily pollutes, brings abnormal flavour and discharge after stain environment to Wheat Sprout Powder.

Chinese patent CN103504216B discloses a kind of Fructus Hordei Germinatus rich in selenium flour producing process and machine for plant cultivation without soil, it is desirable to provide a kind of production process easily holds, the Fructus Hordei Germinatus powder Se content that produces is high Fructus Hordei Germinatus rich in selenium flour producing process and machine for plant cultivation without soil.Described processing technique comprises the following steps: seed soaking, accelerating germination, cultivation, cleaning, dries, pulverize, sieve, sterilize, pack.Patent soak Components Chemical composition disclosed above is many, containing gibberellins---antibiotic, serious environment pollution and there is food safety hidden danger, equally exist immersion and germinating time is long, efficiency is low, the defect that cost is high, adopts the high-temperature baking of 100-120 DEG C simultaneously, and the bioactive substance loss of Fructus Hordei Germinatus is bigger.

The preparation method that Chinese patent CN102132838A discloses a kind of puffed chromium-enriched tartary buckwheat malt powder, after Radix Et Rhizoma Fagopyri Tatarici soaks 24 hours in 100mg/L chromium methionine solution, put into basket to germinate, and in germination process every 4 hours with 100mg/L chromium methionine solution spraying once, when sprout grows to 1-2 centimetre, washing, shell, 60-80 DEG C of hot air drying is to water content 15-20%,, pulverizing expanded with extruder 140 DEG C, obtains puffed chromium-enriched tartary buckwheat malt powder.The present invention maintains the original nutritional labeling of Radix Et Rhizoma Fagopyri Tatarici and biological flavone medicinal efficacy, and chrome content 10mg/kg, without inorganic chromium.Chromium supplement food additive can be made and prevent and treat the functional food of diabetes, hyperlipidemia, Hypertensive Population, evident in efficacy.Patent disclosed above is soaked and germinating time is long, and efficiency is low, and cost is high, and soak easily pollutes, brings abnormal flavour and discharge after stain environment to tartary buckwheat malt powder；High temperature puffing will also result in the loss of Fructus Hordei Germinatus bioactive substance simultaneously.

The less of Fructus Tritici aestivi composite powder is prepared for raw material with Semen Tritici aestivi:

Chinese patent CN103039863B discloses the Fructus Hordei Germinatus powder of a kind of clearing away heat-damp and promoting diuresis, including Fructus Momordicae charantiae superfine powder, Semen Coicis superfine powder and Fructus Hordei Germinatus powder；Wherein the weight portion of Fructus Momordicae charantiae superfine powder is 5～15, and the weight portion of Semen Coicis superfine powder is 10～20, and the weight portion of Fructus Hordei Germinatus powder is 65～85；The method have the benefit that heat-clearing and toxic substances removing, circulation of qi promoting helps digestion, clearing away heat-damp and promoting diuresis, continuous spirit, keeps health.Patent disclosed above have employed the mode of low temperature stage drying when preparing Fructus Hordei Germinatus, but does not disclose concrete germination process.

To sum up, with Semen Tritici aestivi for primary raw material, soak and germinating time is short, efficiency is high, do not result in environmental pollution and soak not easily pollute, spoiled, a kind of mouthfeel of final preparation and color and luster are natural, and nutritional labeling and bioactive substance content are high, and the significant probiotic bacteria Fructus Tritici aestivi composite powder of component strong health-care effect simple, functional is still a kind of necessary.

Summary of the invention

Solved by the invention technical problem is that the defect overcoming existing Wheat Sprout Powder and preparation thereof, with Semen Tritici aestivi for primary raw material, add probiotic powerful Lactobacillus plantarum powder, ferment powder, and the functional raw material such as the composite modified dietary fiber of science, oligosaccharide, soak and germinating time is short, efficiency is high, do not result in environmental pollution and soak not easily pollute, spoiled, a kind of mouthfeel of final preparation and color and luster are natural, nutritional labeling and bioactive substance content are high, component strong, the significant probiotic bacteria Fructus Tritici aestivi composite powder of health-care effect simple, functional.

In order to achieve the above object, the present invention is by the following technical solutions:

A kind of probiotic bacteria Fructus Tritici aestivi composite powder, is mainly prepared by the raw material of following parts by weight:

Wheat Sprout Powder 40-60 part, modified dietary fiber 18-28 part, Lactobacillus plantarum powder 6-12 part, ferment powder 5-10 part, oligosaccharide 5-10 part；

Preferably, described probiotic bacteria Fructus Tritici aestivi composite powder, mainly prepared by the raw material of following parts by weight:

Wheat Sprout Powder 45-55 part, modified dietary fiber 21-25 part, Lactobacillus plantarum powder 8-10 part, ferment powder 7-9 part, oligosaccharide 6-8 part；

It is highly preferred that described probiotic bacteria Fructus Tritici aestivi composite powder, mainly prepared by the raw material of following parts by weight:

Wheat Sprout Powder 50 parts, modified dietary fiber 23 parts, 9 parts of Lactobacillus plantarum powder, ferment powder 8 parts, oligosaccharide 7 parts；

Further, described Wheat Sprout Powder is with Semen Tritici aestivi for primary raw material, prepares through techniques such as ultrasonic cleaning, the instantaneous process of microwave, the immersion of ultrasonic wave added biological enzymolysis, germination, dry, low-temperature grinding successively；

nullPreferably，The preparation method of described Wheat Sprout Powder，Comprise the steps: to be placed on by Semen Tritici aestivi in the ultrasonic washing unit equipped with 0.01-0.03% sodium bicarbonate solution in power 200-400W、Frequency 20-30KHz room temperature ultrasonic cleaning 3-5min，Take out、Drain，In frequency 2450MHz、Power 3000W、Temperature 38-42 DEG C、Thickness of feed layer 2-4cm microwave drying 10-15s,Then put temperature and be 33-36 DEG C、PH value be 6-8 soak in soak 4-6h，Soak contains the compound enzyme that mass percent concentration is 0.15-0.25%，Ventilate once every 15-20min，Ventilation pressure 0.13-0.15MPa，Simultaneously in electric field intensity 10-15kV/cm，Burst length 100-300 μ s,Pulse frequency 60-80Hz condition carries out high voltage pulse electric field processing，Until wheat water content content is 40-45%；Semen Tritici aestivi after immersion drains, and carries out dark germination in growing floor, and dark germination temperature keeps 20-24 DEG C, and the dark germination time is 20-24h, and wheat drying to the moisture after germinateing is 5-8%, low-temperature grinding, crosses 60-80 mesh sieve and namely obtains Wheat Sprout Powder；

Described compound enzyme is cellulase, 1,4 beta-glucanase, xylanase 2-4:2-4:1-3 Homogeneous phase mixing in mass ratio.

Further, described modified dietary fiber is that through physics, chemical or biological method having of processing and obtain, the abundant soluble fiber cellulose content of strong retentiveness, dilatancy, thickening property, adsorptivity and network is high, biological activity is strong, human body beneficial flora has cellulose important, positive role by dietary fiber, compared with full diet fiber, its biological action is more powerful, also can be greatly prolonged the shelf-life of grape fruit powder；

Preferably, described modified fibre is to be obtained through enzyme enzymolysis by one or more in inulin, apple fiber, Herba avenae fatuae fiber, Semen Tritici aestivi fiber；

More preferably, the preparation method of described modified dietary fiber, comprise the following steps: by inulin, Semen Tritici aestivi fiber, Herba avenae fatuae fiber 5-7:2-4:1-3 Homogeneous phase mixing in mass ratio, add its water of quality 3-7 times, room temperature 100-300W, 35-40KHz condition supersound extraction 10-15min, then at electric field intensity 20-40kV/cm, burst length 300-500 μ s, carry out high voltage pulse electric field processing 10-15min when pulse frequency 200-400Hz；It is 4.5-6.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min；Enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are that namely 5-8% obtains modified dietary fiber；

Described enzyme is cellulase, xylanase, laccase, pectase 1-3:1-3:1-2:1-2 Homogeneous phase mixing in mass ratio.

Further, described Lactobacillus plantarum powder is prepared with Lactobacillus plantarum CGMCCNO.11763 according to a conventional method for starting strain, and its viable bacteria content is: 7 × 10¹²-9×10¹²cfu/g；The cryoprotective agent that wherein freeze drying process adopts is with the animal or plant containing antifreeze protein for raw material; prepared through high-pressure pulse electric extraction, ultrasonic assistant microwave extraction and compound enzyme enzymolysis, Lactobacillus plantarum powder can be effectively improved at freezing dry process Viable detection；

Preferably, described protectant preparation method, comprise the steps: winter rye, Caulis et Folium Ammopiptanthi Mongolici, sharkskin collagen protein is respectively washed, drain, 8-10:3-5:2-4 Homogeneous phase mixing in mass ratio, add the lactic acid moistening 3-8h that pH value is 3.8-4.5 of mixed material quality 0.1-1 times, pulverize immediately after-18--22 DEG C of freezing 1-2h, freezing thickness of feed layer 3-5cm, ground product particle diameter 0.5-3mm, it is subsequently added into the water of ground product quality 10-20 times, it is 3.5-5.5 with breast acid for adjusting pH value, at electric field intensity 25-35kV/cm under room temperature, burst length 300-500 μ s, high voltage pulse electric field processing 20-30min is carried out when pulse frequency 200-300Hz；Then carry out microwave irradiation and extraction 15-20min when power 150-300W in room temperature, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction；Add the compound enzyme of extracting solution quality 1-2%, in 45-55 DEG C of enzymolysis 30-50min；Enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that namely 0.1-0.3mm obtains protective agent；

Described compound enzyme is cellulase, protease, amylase, pectase, tannase 2-4:1-3:1-3:1-2:1-2 Homogeneous phase mixing in mass ratio；

Extract it is highly preferred that described microwave irradiation and extraction is batch (-type), i.e. microwave exposure 10s, interval 20s.

Further, described ferment powder preparation method is as follows: adopting the fermentation liquid that pickle fermentation produces discharge in latter stage is raw material, being filtered to remove the big grain materials such as the dish leaf in fermentation liquid and obtain fermented liquid, fermented liquid adopts the drying meanss such as lyophilization to prepare ferment powder.Cryodesiccated condition is :-30--40 degree pre-freeze 6 hours；Then lyophilizing is until water content is less than 5%.Pickle production method is with reference to Chinese patent 201110421967.3 or 201210310308.7 preparation.

Further, described oligosaccharide is at least one in oligofructose, stachyose, Raffinose, oligomeric xylose, oligomeric galactose, soybean oligo saccharide, oligomeric isomaltose, Oligomeric maltose；

Preferably, described oligosaccharide parts by weight consist of oligofructose 40-50 part, Oligomeric maltose 30-40 part, Raffinose 20-40 part, soybean oligo saccharide 16-18 part, oligomeric galactose 10-15 part, oligomeric xylose 10-15 part, oligomeric isomaltose 10-15 part, stachyose 8-12 part.

The preparation method that another object of the present invention is to provide above-mentioned probiotic bacteria Fructus Tritici aestivi composite powder, comprise the steps: according to formula proportion, weigh each raw material, successively ferment powder, Lactobacillus plantarum powder, Wheat Sprout Powder, oligosaccharide are added V-type blending tank Homogeneous phase mixing 25-35min, speed of agitator 20-40r/min, being subsequently adding modified dietary fiber uniformly mixed 12-18min, speed of agitator 40-60r/min, namely sterile filling, sealing, packaging obtain probiotic bacteria Fructus Tritici aestivi composite powder；

Preferably, described sterile filling adopts automatic powdery filling machine fill, adopts simultaneously⁶⁰Co irradiation sterilization, irradiation dose 4kGy；

Preferably, described Wheat Sprout Powder adopts Aluminum-plastic composite bag fill；Ultraviolet or ozonization 20-40min is adopted before described Aluminum-plastic composite bag fill.

The Fructus Tritici aestivi composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 6.84 × 10¹¹-9.49×10¹¹cfu/g。

Lactobacillus plantarum of the present invention (Lactobacillusplantarum) XH is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (being called for short CGMCC) on November 30th, 2015, preserving number is CGMCCNO.11763, preservation address is: North Star West Road 1, Chaoyang District, city of BeiJing, China institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101.

Lactobacillus plantarum probiotic properties is as follows:

Lactobacillus plantarum CGMCCNO.11763 provided by the present invention is found to survive when pH is 1.50 through experiment, still in existing state after 1% cholate is cultivated 4 hours；Lactobacillus plantarum CGMCCNO.11763 degrading nitrite speed is fast, and capacity of decomposition reaches 10.9mg/h/kg, and this strain is when producing Pickles, and whole sweat nitrite concentration is at below 4.8mg/kg；Degrading rate of cholesterol, after fermentation 60h hour, can be reached 64.76% by CGMCCNO.11763.CGMCCNO.11763 Adhering capacity measure from coagulation rate be 95.71%.

Lactobacillus plantarum CGMCCNO.11763 is to cholesterol degradation capability study and mensuration:

Take 1mlCGMCCNO.11763 mother solution and be inoculated in MRS cholesterol fluid medium (the cholesterol level 0.1mg/ml of 10mL, pH6.2) in, the constant temperature of 37 DEG C stands and cultivates 20h respectively, 40h, 60h is standby, with the MRS cholesterol culture medium that accesses 1mL sterilized water for comparison, take bacteria liquid sample and the comparison each 1ml of liquid of above cultivation different time, 9000r/min, centrifugal 10min at 4 DEG C, obtain fermented supernatant fluid, in o-phthalaldehyde method mensuration supernatant, cholesterol level is (particularly as follows: take each supernatant 0.1ml in corresponding test tube, add glacial acetic acid 0.3ml, the o-phthalaldehyde(OPA) 0.15ml of 1mg/ml, it is slowly added into concentrated sulphuric acid 1.0ml, mix homogeneously.Room temperature stands 10min, surveys light absorption value under 550nm).Each process 3 repetition, in kind makes cholesterol standard curve, calculates cholesterol level and degradation rate in supernatant, and result is in Table 1.It can be seen that cholesterol is had good Degradation by CGMCCNO.11763, after fermentation 60h hour, degradation rate can reach 64.76%.

The table 1 degraded situation to cholesterol.

Degradation time (h)	0	20h	40h	60h
					Cholesterol level (mg/ml)	0.2273±0.0058	0.1356±0.0018	0.1011±0.0094	0.801±0.0231
Degrading rate of cholesterol %		40.34%	55.52%	64.76%

The bile tolerance test of Lactobacillus plantarum CGMCCNO.11763 bacterial strain:

Take CGMCCNO.11763 bacterium solution 1mL and inoculate strain in the 10mLMRS fluid medium (PH=6.4) containing different cholate (concentration gradients is 0.0%, 0.2%, 0.4%, 0.6%, 0.8%, 1%), be placed at 37 DEG C to cultivate respectively 0,2,4h, each process 3 repetition.Respectively take 1ml sample bacterium solution to mix in 9ml normal saline, prepare dilution factor solution, take 0.1ml diluent to be coated with in MRS, be inverted in 37 DEG C of biochemical cultivation cases and cultivate 48 hours (each dilution factor do 3 parallel) record and calculate the several number of bacterium on flat board.Result is in Table 2.This bacterium known increment of bacterium after gallbladder salinity is 1% process 4h still reaches 0.59 ± 0.92 × 10⁷(cfu/ml), there is good bile tolerance ability.

Table 2 bile tolerance ability detection [(± s) × 10⁷cfu/ml]

The acid resistance test of Lactobacillus plantarum CGMCCNO.11763 bacterial strain

Take HLX37 mother solution and inoculate strain in the 10mLMRS fluid medium of different pH value (pH gradient is 1.5,2.0,2.5,3.0,3.5,4.0) by 1ml, be placed at 37 DEG C to cultivate respectively 0,2,4h, each process 3 repetition.Respectively take 1ml sample bacterium solution to mix in 9ml normal saline, prepare dilute solution, take 0.1ml diluent and be coated with in MRS, in 37 DEG C of biochemical cultivation cases, be inverted the bacterium colony number cultivated on 48 hours (each dilution factor do 3 parallel) record flat board.Result is in Table 3.Illustrate that this bacterium has very strong acid-fast ability.

Table 3 acid-fast ability detection [(± s) × 10⁷cfu/ml]

The Adhering capacity of Lactobacillus plantarum CGMCCNO.11763 measures

Cultivate CGMCCNO.11763 (MRS fluid medium), bacillus coli DH 5 alpha (LB fluid medium) 24h obtains fermentation liquid, be respectively placed in 3000r/min, centrifugal 10min at 4 DEG C, collect bacterium mud, wash bacterium mud 2 times with the sterile phosphate buffer (PBS) of pH=7.0 respectively and (in bacterium colony, namely add PBS, after concussion mix homogeneously, be placed in 3000r/min, centrifugal 10min at 4 DEG C, collect thalline).From coagulation rate (%): with aseptic PBS, bacterium mud CGMCCNO.11763 to be formed in the suspension bacteria liquid that light absorption value is 0.4 ± 0.1 (A0) and the bacteria suspension at wavelength 600nm place, measure light absorption value A24 after standing 24h, be (A0 A24)/A0 from coagulation rate (%) formula.；His coagulation rate (%): the outstanding bacterium solution of CGMCCNO.11763 and bacillus coli DH 5 alpha is adjusted to the mix suspending bacterium solution that the light absorption value at wavelength 600nm place is 0.6 ± 0.1 (A0).Measuring light absorption value A24 after standing 24H, his coagulation rate (%) formula is (A0 A24)/A0.Measurement result is in Table 5, it is known that CGMCCNO.11763 is 95.71% from coagulation rate, has very strong Adhering capacity.

Table 4 Adhering capacity table

The bacterial strain physiological property of Lactobacillus plantarum CGMCCNO.11763

Described Lactobacillus plantarum (Lactobacillusplantarum) XH is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (being called for short CGMCC) on November 30th, 2015, preserving number is CGMCCNO.11763, preservation address is: North Star West Road 1, Chaoyang District, city of BeiJing, China institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101.

This bacterial strain feature is as follows: examine under a microscope, and this bacterial strain is rod-short, and Gram’s staining is positive, atrichia, does not produce spore；On solid medium, this bacterium bacterium colony is white, and smooth surface is fine and close, and form is circular, and edge is more neat.

Physicochemical characteristics is: catalase (-), gelatin liquefaction (-), indole experiment (+), mobility (-), fermentation gas (-), nitrate reductase (-), fermentation gas (-), product hydrogen sulfide gas (-), pH4.0MRS culture medium grows (+).It is accredited as Lactobacillus plantarum (Lactobacillusplantarum), called after Lactobacillus plantarum (Lactobacillusplantarum) XH through Physiology and biochemistry.

Bacterial strain can at 57 DEG C well-grown, glucose tolerance is 275g/L.

Lactobacillus plantarum of the present invention, by gathering people Li Jianshu, separates in Yoghourt from Xinjiang Uygur fellow-villager family and obtains, acquisition time on June 2nd, 2015.

Lactobacillus plantarum CGMCCNO.11763 of the present invention is found to survive when pH is 1.50 through experiment, still in existing state after 1% cholate is cultivated 4 hours；Lactobacillus plantarum CGMCCNO.11763 degrading nitrite speed is fast, and capacity of decomposition reaches 10.9mg/h/kg, and this strain is when producing Pickles, and whole sweat nitrite concentration is at below 4.8mg/kg；Degrading rate of cholesterol, after fermentation 60h hour, can be reached 64.76% by CGMCCNO.11763.CGMCCNO.11763 Adhering capacity measure from coagulation rate be 95.71%, bacterial strain can at 57 DEG C well-grown, glucose tolerance is 275g/L.

Beneficial effect:

Probiotic bacteria Fructus Tritici aestivi composite powder of the present invention will have natural colored, the Wheat Sprout Powder of local flavor and mouthfeel and Lactobacillus plantarum powder, modified dietary fiber, ferment powder, the functional product science such as oligosaccharide are composite, not only it is effectively increased the trophism of Fructus Tritici aestivi composite powder, foodsafety and health care, and significantly improve the probiotic of Fructus Tritici aestivi composite powder and quality stability, compared with existing Fructus Tritici aestivi composite powder: its component is simple, color pool, local flavor and mouthfeel are natural, it is of high nutritive value, health care is strong, bioactive substance content is high, probiotics viable bacteria quantity is high, probiotic by force, probiotic bacteria functional metabolic species is complete, content is high, foodsafety is high, long shelf-life.Wherein Fructus Tritici aestivi preparation process adopts ultrasonic cleaning, microwave instantaneous expanded to kill embryo, high-voltage pulse, biological enzymolysis combine immersion, accelerating germination, soak and germinating time is short, germination percentage is high, bud length is homogeneous, bioactive substance content is high, production efficiency is high, do not result in environmental pollution and soak not easily pollute, spoiled, environmentally friendly.Concrete test effect is shown in embodiment seven-ten two, and concrete component effect is as follows:

1. the Lactobacillus plantarum CGMCCNO.11763 of the present invention is found to survive when pH is 1.50 through experiment, still in existing state after 1% cholate is cultivated 4 hours；Lactobacillus plantarum CGMCCNO.11763 degrading nitrite speed is fast, and capacity of decomposition reaches 10.9mg/h/kg, and this strain is when producing Pickles, and whole sweat nitrite concentration is at below 4.8mg/kg；Degrading rate of cholesterol, after fermentation 60h hour, can be reached 64.76% by CGMCCNO.11763.CGMCCNO.11763 Adhering capacity measure from coagulation rate be 95.71%.

2. the cryoprotective agent that the present invention adopts when preparing Lactobacillus plantarum powder is by composite to the winter rye higher containing antifreeze protein, Caulis et Folium Ammopiptanthi Mongolici and sharkskin collagen protein science; prepare through high-pressure pulse electric extraction, ultrasonic assistant microwave extraction and biological enzymolysis; omnidistance extract at low temperature; antifreeze protein extraction ratio is high; loss is few; the protective agent antifreeze peptide content obtained is high, kind is complete, functional by force, cryoprotective effects is good, improves the viable bacteria content in Lactobacillus plantarum powder.

3. the modified dietary fiber that prepared by the present invention is that dietary fiber is processed through physics, chemical or biological method and obtained, soluble fiber cellulose content is high, biological activity is strong, beneficial bacteria of intestinal tract group has cellulose important, positive role, compared with full diet fiber, its biological action is more powerful: 1) soluble fiber cellulose content is high, it is easier to be utilized by probiotic bacteria, probiotic bacteria can be improved in the growth of intestinal and fertility, increase kind and the quantity of probiotic bacteria flora, reduce large intestine pH value, improve intestine microenvironment；2) powerful absorbability, after modified, cellulosic specific surface area increases, and network is enriched, and absorption affinity strengthens, and the organic molecule ability of chelating, absorption cholesterol and bile acids is higher, suppress the human body absorption to them；3) ion-exchange capacity strengthens, and to metallic element, particularly heavy metal element adsorption effect is higher；4) retentiveness, dilatancy, thickening property be higher and acid and alkali, alkali, salt impact, 5) resident time of adjustment and maintenance intestinal microbial population, strengthen digestion and the absorbability of intestinal, improve body immunity, finally significantly improve digestion and the absorbance of Wheat Sprout Powder effective ingredient；6) promote gastrointestinal peristalsis, slow down and eliminate the untoward reaction such as flatulence, abdominal distention；7) embedding effect is strong, can effectively prevent external environment (oxygen, temperature, illumination, the humidity etc.) factor impact on product quality, stabilize the biological activity of product, extend the shelf-life of product.

null4. the Semen Tritici aestivi of immersion process is carried out ultrasonic cleaning by the preparation method of Wheat Sprout Powder of the present invention、The instantaneous process of microwave and high voltage pulse electric field processing effectively prevent soak pollution microbes，Avoid generation stink to penetrate in Wheat Sprout Powder product，Simultaneously，Can effectively strengthen the relative permeability of corn seed coat cell wall and cell membrane、Improve the water absorption rate of full cereal seed，Promote that seed is sprouted in advance，Improve generation speed and adenosine triphosphate (ATP) content of ultra-oxygen anion free radical，The activation of the multiple enzyme of stratification early stage wheat seed and release、Endosperm dissolves and functional nutrient composition、Bioactive ingredients、The synthesis of antioxidant content，Promote the respiratory metabolism of seed，Accelerate nutrition and functional materials、Bioactive ingredients、The enrichment process of antioxidant content，Shorten enrichment time，Improve nutrition and functional materials、Bioactive ingredients、The content of antioxidant content，Organically combine with biological enzymolysis，Can degrade further seed coat cellulose and hemicellulose structure，Increase seed coat permeability，Activate various bioactive substance (endogenous enzymes etc.) vigor the strongest，Enriching quantity is bigger，Soluble fiber cellulose content increases therewith，Trophic factors is provided for the Lactobacillus plantarum in Wheat Sprout Powder，Effect is more preferably notable；Ultrasonic, microwave, high-voltage pulse electric field technology and biological enzymolysis are organically combined, shortens soak time, improve germination percentage and germination uniformity；Wheat Sprout Powder heat-sensitive substance content can be kept to greatest extent, especially antioxidant (glutathion, inositol hexaphosphate, vitamin C, polyphenol etc.), ensure the natural colored of Wheat Sprout Powder, taste and flavor to greatest extent, may also function as bactericidal action simultaneously；Particularly after the instantaneous appropriateness of microwave kills embryo, in the bioactive situation not affecting Fructus Tritici aestivi seed, absorption along with moisture, wheat embryo be suppressed but do not affect the growth of every enzyme and bioactive substance and the dissolving of endosperm, blastogenesis length is very short, Repiration is weak, sprout damage is low, improve yield and the quality of Wheat Sprout Powder, significantly improve in Wheat Sprout Powder product functional, trophism, the content of bioactive substance, the health care that improve Wheat Sprout Powder (improves body immunity, remove oxygen-derived free radicals in human body, blood fat reducing, slow down aging), nutritive value and foodsafety.The Fructus Tritici aestivi germination percentage of preparation reaches more than 97%, the long 0.2-0.5mm of bud, length is homogeneous, sprout damage is only 1.5-2.1%, reducing 4.8-7.1% than the loss of existing germination technology, functional materials content is high, and wherein alpha-aminobutyric acid content is 303.8-315.6mg/100g, glutathion 16.1-18.3mg/100g, inositol hexaphosphate (IP₆) 460.8-484.4mg/100g, dietary fiber 3.7-4.2mg/100g.

5. the ferment powder that prepared by the present invention does not contain only the purebred probiotic bacterias such as acetobacter, bacillus bifidus, yeast and lactobacillus rhamnosus, and containing more, more useful in pickle fermentation process participate in and produce multiple wild microbial strains, its biological activity and probiotic more comprehensively and powerful, composite with Lactobacillus plantarum powder science prepared by the present invention, its effect is more significantly.

6. the preparation method technique of Fructus Tritici aestivi composite powder of the present invention is simple, easy to operate, low for equipment requirements; can industrialization and large-scale production; modified dietary fiber is finally mixed, has played its powerful embedding effect so that the character of product is more stable, the shelf-life is longer.

What it should be noted that probiotic bacteria Fructus Tritici aestivi composite powder of the present invention has the technical effect that the result that each component technical characteristic and method feature are mutually worked in coordination with, interacted, the not superposition of simple technical characteristic (component function), the combination of each component technical characteristic and the collaborative effect produced, considerably beyond the superposition of each monotechnics feature functionality and effect, have advanced preferably and practicality.

Detailed description of the invention

The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art.It addition, embodiment is interpreted as illustrative, and unrestricted the scope of the present invention, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, under the premise without departing substantially from spirit and scope of the present invention, the various changes or the changes that carry out the material component in these embodiments and consumption fall within protection scope of the present invention.

Embodiment one:

Prepared by raw material

1, the preparation of modified dietary fiber

Its preparation method comprises the following steps:

By inulin, Semen Tritici aestivi fiber, Herba avenae fatuae fiber 6:3:2 Homogeneous phase mixing in mass ratio, add the water of its quality 5 times, room temperature 200W, 38KHz condition supersound extraction 13min, then at electric field intensity 30kV/cm, burst length 400 μ s, carries out high voltage pulse electric field processing 13min when pulse frequency 300Hz；It is 5.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.2%, in 50 DEG C of enzymolysis 34min；Enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are 6.5% namely obtain modified dietary fiber；

Above-mentioned enzyme is cellulase, xylanase, laccase, pectase 2:2:1.5:1.5 Homogeneous phase mixing in mass ratio.

2, the preparation of Lactobacillus plantarum powder

Lactobacillus plantarum powder is prepared with Lactobacillus plantarum CGMCCNO.11763 according to a conventional method for starting strain, and its viable bacteria content is: 8 × 10¹²cfu/g；The cryoprotective agent that wherein freeze drying process adopts is with the animal or plant containing antifreeze protein for raw material; prepared through high-pressure pulse electric extraction, ultrasonic assistant microwave extraction and compound enzyme enzymolysis, Lactobacillus plantarum powder can be effectively improved at freezing dry process Viable detection；

Above-mentioned protectant preparation method, comprises the steps:

Winter rye, Caulis et Folium Ammopiptanthi Mongolici, sharkskin collagen protein are respectively washed, drain, 9:4:3 Homogeneous phase mixing in mass ratio, add the lactic acid moistening 6h that pH value is 4.2 of mixed material quality 0.5 times, pulverize immediately after-20 DEG C of freezing 1.5h, freezing thickness of feed layer 4cm, ground product particle diameter 2mm, it is subsequently added into the water of ground product quality 15 times, it is 4.5 with breast acid for adjusting pH value, at electric field intensity 30kV/cm under room temperature, burst length 400 μ s, carries out high voltage pulse electric field processing 25min when pulse frequency 250Hz；Then microwave irradiation and extraction 18min is carried out in room temperature when power 225W, its microwave exposure 10s, interval 20s, simultaneously at power 250W, when frequency 35KHz, carry out ultrasonic assistant extraction；Add the compound enzyme of extracting solution quality 1.5%, in 50 DEG C of enzymolysis 40min；Enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that namely 0.2mm obtains protective agent；

Above-mentioned compound enzyme is cellulase, protease, amylase, pectase, tannase 3:2:2:1.5:1.5 Homogeneous phase mixing in mass ratio.

3, the preparation of ferment powder

Its preparation method is as follows:

Adopting the fermentation liquid that pickle fermentation produces discharge in latter stage is raw material, is filtered to remove the big grain materials such as the dish leaf in fermentation liquid and obtains fermented liquid, and fermented liquid adopts the drying meanss such as lyophilization to prepare ferment powder.Cryodesiccated condition is :-35 degree pre-freezes 6 hours；Then lyophilizing is until water content 3%.Pickle production method is prepared with reference to Chinese patent 201110421967.3.

Modified dietary fiber that following example two to six use, Lactobacillus plantarum powder, ferment powder are by embodiment one preparation.

Embodiment two:

Wherein oligosaccharide parts by weight consist of oligofructose 45 parts, Oligomeric maltose 35 parts, Raffinose 30 parts, soybean oligo saccharide 17 parts, oligomeric galactose 13 parts, oligomeric xylose 13 parts, oligomeric isomaltose 13 parts, 10 parts of stachyose；

Wherein the preparation method of Wheat Sprout Powder, comprises the steps:

Semen Tritici aestivi is placed in the ultrasonic washing unit equipped with 0.02% sodium bicarbonate solution in power 300W, frequency 25KHz room temperature ultrasonic cleaning 4min, take out, drain, in frequency 2450MHz, power 3000W, temperature 40 DEG C, thickness of feed layer 3cm microwave drying 13s, then putting temperature is 35 DEG C, pH value be 7 soak in soak 5h, soak contains the compound enzyme that mass percent concentration is 0.20%, ventilate once every 18min, ventilation pressure 0.14MPa, simultaneously in electric field intensity 13kV/cm, burst length 200 μ s, pulse frequency 70Hz condition carries out high voltage pulse electric field processing, until wheat water content content is 43%；Semen Tritici aestivi after immersion drains, and carries out dark germination in growing floor, and dark germination temperature keeps 22 DEG C, and the dark germination time is 22h, and wheat drying to the moisture after germinateing is 6.5%, low-temperature grinding, crosses 70 mesh sieves and namely obtains Wheat Sprout Powder；

Above-mentioned compound enzyme is cellulase, 1,4 beta-glucanase, xylanase 3:3:2 Homogeneous phase mixing in mass ratio.

The preparation method of probiotic bacteria Fructus Tritici aestivi composite powder, comprises the steps:

According to formula proportion, weigh each raw material, successively ferment powder, Lactobacillus plantarum powder, Wheat Sprout Powder, oligosaccharide are added V-type blending tank Homogeneous phase mixing 30min, speed of agitator 30r/min, it is subsequently adding modified dietary fiber uniformly mixed 15min, speed of agitator 50r/min, namely sterile filling, sealing, packaging obtain probiotic bacteria Fructus Tritici aestivi composite powder；

Above-mentioned sterile filling adopts automatic powdery filling machine fill, adopts simultaneously⁶⁰Co irradiation sterilization, irradiation dose 4kGy；

Above-mentioned Wheat Sprout Powder adopts Aluminum-plastic composite bag fill；Disinfection by ultraviolet light 30min is adopted before described Aluminum-plastic composite bag fill.

The probiotic bacteria Fructus Tritici aestivi composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 9.49 × 10¹¹cfu/g。

Embodiment three:

Wheat Sprout Powder 45 parts, modified dietary fiber 21 parts, 8 parts of Lactobacillus plantarum powder, ferment powder 7 parts, wheat oligosaccharide 6 parts；

Wherein oligosaccharide parts by weight consist of oligofructose 40 parts, Oligomeric maltose 30 parts, Raffinose 20 parts, soybean oligo saccharide 16 parts, oligomeric galactose 10 parts, oligomeric xylose 10 parts, oligomeric isomaltose 10 parts, 8 parts of stachyose；

Wherein the preparation method of Wheat Sprout Powder, comprises the steps:

Semen Tritici aestivi is placed in the ultrasonic washing unit equipped with 0.01% sodium bicarbonate solution in power 200W, frequency 20KHz room temperature ultrasonic cleaning 3min, take out, drain, in frequency 2450MHz, power 3000W, temperature 38 DEG C, thickness of feed layer 2cm microwave drying 10s, then putting temperature is 33 DEG C, pH value be 6 soak in soak 4h, soak contains the compound enzyme that mass percent concentration is 0.15%, ventilate once every 15min, ventilation pressure 0.13MPa, simultaneously in electric field intensity 10kV/cm, burst length 100 μ s, pulse frequency 60Hz condition carries out high voltage pulse electric field processing, until wheat water content content is 40%；Semen Tritici aestivi after immersion drains, and carries out dark germination in growing floor, and dark germination temperature keeps 20 DEG C, and the dark germination time is 20h, and wheat drying to the moisture after germinateing is 5%, low-temperature grinding, crosses 60 mesh sieves and namely obtains Wheat Sprout Powder；

Above-mentioned compound enzyme is cellulase, 1,4 beta-glucanase, xylanase 2:2:1 Homogeneous phase mixing in mass ratio.

According to formula proportion, weigh each raw material, successively ferment powder, Lactobacillus plantarum powder, Wheat Sprout Powder, oligosaccharide are added V-type blending tank Homogeneous phase mixing 25min, speed of agitator 20r/min, it is subsequently adding modified dietary fiber uniformly mixed 12min, speed of agitator 40r/min, namely sterile filling, sealing, packaging obtain probiotic bacteria Fructus Tritici aestivi composite powder；

Above-mentioned Wheat Sprout Powder adopts Aluminum-plastic composite bag fill；Disinfection by ultraviolet light 20min is adopted before described Aluminum-plastic composite bag fill.

The probiotic bacteria Fructus Tritici aestivi composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 9.02 × 10¹¹cfu/g。

Embodiment four:

Wheat Sprout Powder 55 parts, modified dietary fiber 25 parts, 10 parts of Lactobacillus plantarum powder, ferment powder 9 parts, wheat oligosaccharide 8 parts；

Wherein oligosaccharide parts by weight consist of oligofructose 50 parts, Oligomeric maltose 40 parts, Raffinose 40 parts, soybean oligo saccharide 18 parts, oligomeric galactose 15 parts, oligomeric xylose 15 parts, oligomeric isomaltose 15 parts, 12 parts of stachyose；

Wherein the preparation method of Wheat Sprout Powder, comprises the steps:

Semen Tritici aestivi is placed in the ultrasonic washing unit equipped with 0.03% sodium bicarbonate solution in power 400W, frequency 30KHz room temperature ultrasonic cleaning 5min, take out, drain, in frequency 2450MHz, power 3000W, temperature 42 DEG C, thickness of feed layer 4cm microwave drying 15s, then putting temperature is 36 DEG C, pH value be 8 soak in soak 6h, soak contains the compound enzyme that mass percent concentration is 0.25%, ventilate once every 20min, ventilation pressure 0.15MPa, simultaneously in electric field intensity 15kV/cm, burst length 300 μ s, pulse frequency 80Hz condition carries out high voltage pulse electric field processing, until wheat water content content is 45%；Semen Tritici aestivi after immersion drains, and carries out dark germination in growing floor, and dark germination temperature keeps 24 DEG C, and the dark germination time is 24h, and wheat drying to the moisture after germinateing is 8%, low-temperature grinding, crosses 80 mesh sieves and namely obtains Wheat Sprout Powder；

Above-mentioned compound enzyme is cellulase, 1,4 beta-glucanase, xylanase 4:4:3 Homogeneous phase mixing in mass ratio.

According to formula proportion, weigh each raw material, successively ferment powder, Lactobacillus plantarum powder, Wheat Sprout Powder, oligosaccharide are added V-type blending tank Homogeneous phase mixing 35min, speed of agitator 40r/min, it is subsequently adding modified dietary fiber uniformly mixed 18min, speed of agitator 60r/min, namely sterile filling, sealing, packaging obtain probiotic bacteria Fructus Tritici aestivi composite powder；

Above-mentioned Wheat Sprout Powder adopts Aluminum-plastic composite bag fill；Disinfection by ultraviolet light 40min is adopted before described Aluminum-plastic composite bag fill.

The probiotic bacteria Fructus Tritici aestivi composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 8.88 × 10¹¹cfu/g。

Embodiment five:

Wheat Sprout Powder 40 parts, modified dietary fiber 18 parts, 6 parts of Lactobacillus plantarum powder, ferment powder 5 parts, oligosaccharide 5 parts；

Wherein oligosaccharide is stachyose；

Wherein the preparation method of Wheat Sprout Powder, comprises the steps:

Semen Tritici aestivi is placed in the ultrasonic washing unit equipped with 0.01% sodium bicarbonate solution in power 400W, frequency 20KHz room temperature ultrasonic cleaning 5min, take out, drain, in frequency 2450MHz, power 3000W, temperature 38 DEG C, thickness of feed layer 4cm microwave drying 10s, then putting temperature is 36 DEG C, pH value be 6 soak in soak 6h, soak contains the compound enzyme that mass percent concentration is 0.15%, ventilate once every 20min, ventilation pressure 0.13MPa, simultaneously in electric field intensity 15kV/cm, burst length 100 μ s, pulse frequency 80Hz condition carries out high voltage pulse electric field processing, until wheat water content content is 40%；Semen Tritici aestivi after immersion drains, and carries out dark germination in growing floor, and dark germination temperature keeps 24 DEG C, and the dark germination time is 20h, and wheat drying to the moisture after germinateing is 8%, low-temperature grinding, crosses 60 mesh sieves and namely obtains Wheat Sprout Powder；

Above-mentioned compound enzyme is cellulase, 1,4 beta-glucanase, xylanase 2:4:1 Homogeneous phase mixing in mass ratio.

According to formula proportion, weigh each raw material, successively ferment powder, Lactobacillus plantarum powder, Wheat Sprout Powder, oligosaccharide are added V-type blending tank Homogeneous phase mixing 28min, speed of agitator 25r/min, it is subsequently adding modified dietary fiber uniformly mixed 14min, speed of agitator 45r/min, namely sterile filling, sealing, packaging obtain probiotic bacteria Fructus Tritici aestivi composite powder；

Above-mentioned Wheat Sprout Powder adopts Aluminum-plastic composite bag fill；Ozonization 20min is adopted before described Aluminum-plastic composite bag fill.

The probiotic bacteria Fructus Tritici aestivi composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 6.84 × 10¹¹cfu/g。

Embodiment six:

Wheat Sprout Powder 60 parts, modified dietary fiber 28 parts, 12 parts of Lactobacillus plantarum powder, ferment powder 10 parts, oligosaccharide 10 parts；

Wherein oligosaccharide is soybean oligo saccharide；

Wherein the preparation method of Wheat Sprout Powder Wheat Sprout Powder, comprises the steps:

Semen Tritici aestivi is placed in the ultrasonic washing unit equipped with 0.03% sodium bicarbonate solution in power 200W, frequency 30KHz room temperature ultrasonic cleaning 3min, take out, drain, in frequency 2450MHz, power 3000W, temperature 42 DEG C, thickness of feed layer 2cm microwave drying 15s, then putting temperature is 33 DEG C, pH value be 8 soak in soak 4h, soak contains the compound enzyme that mass percent concentration is 0.25%, ventilate once every 15min, ventilation pressure 0.15MPa, simultaneously in electric field intensity 10kV/cm, burst length 300 μ s, pulse frequency 60Hz condition carries out high voltage pulse electric field processing, until wheat water content content is 45%；Semen Tritici aestivi after immersion drains, and carries out dark germination in growing floor, and dark germination temperature keeps 20 DEG C, and the dark germination time is 24h, and wheat drying to the moisture after germinateing is 5%, low-temperature grinding, crosses 80 mesh sieves and namely obtains Wheat Sprout Powder；

Above-mentioned compound enzyme is cellulase, 1,4 beta-glucanase, xylanase 4:2:3 Homogeneous phase mixing in mass ratio.

According to formula proportion, weigh each raw material, successively ferment powder, Lactobacillus plantarum powder, Wheat Sprout Powder, oligosaccharide are added V-type blending tank Homogeneous phase mixing 33min, speed of agitator 35r/min, it is subsequently adding modified dietary fiber uniformly mixed 17min, speed of agitator 55r/min, namely sterile filling, sealing, packaging obtain probiotic bacteria Fructus Tritici aestivi composite powder；

Above-mentioned Wheat Sprout Powder adopts Aluminum-plastic composite bag fill；Ozonization 40min is adopted before described Aluminum-plastic composite bag fill.

The probiotic bacteria Fructus Tritici aestivi composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 7.75 × 10¹¹cfu/g。

Experimental example seven eats the change of T-CHOL after probiotic bacteria Fructus Tritici aestivi composite powder

Selecting the adult 48 of T-CHOL 180mg/dl-250mg/dl, men and women half and half, is randomly divided into three groups；150 milliliters of mineral waters are drunk in first group of dinner every day；Second group of common commercially available Fructus Tritici aestivi composite powder 150g of dinner every day Instant Drinks, the Fructus Tritici aestivi composite powder 150g of the 3rd group of dinner every day Instant Drinks embodiment of the present invention 2, every day period eats same food, and food includes meat, egg, vegetable and fruit.Respectively at the blood of the previous day that experiment starts and the 20th, 40,60 days acquisition test persons, measuring the total cholesterol level in blood, result is table 1 such as:

Table 1: total cholesterol level testing result in blood

Time	0 day	20 days	40 days	60 days
					First group (mg/dl)	202.3	203.8	206.2	209.3
Second group (mg/dl)	207.6	206.5	203.2	202.1
					3rd group (mg/dl)	210.8	203.9	194.6	182.3

As seen from the above table after the Fructus Tritici aestivi composite powder of the Instant Drinks embodiment of the present invention 2, the content generation significant change of the T-CHOL in adult's blood.Compared with common commercially available Fructus Tritici aestivi composite powder, the content of the T-CHOL in adult's blood is significantly reduced by Fructus Tritici aestivi composite powder of the present invention, and the content of the T-CHOL in mineral water composition year human blood significantly increases, although Commercial wheat bud composite powder decreases, but compared with product of the present invention, effect is notable, it follows that the present invention adopts Fructus Tritici aestivi composite powder prepared by the specific bacterial strain with reduction cholesterol characteristic to have the health-care effect well reducing cholesterol.

It should be understood that the probiotic bacteria Fructus Tritici aestivi composite powder prepared by embodiment of the present invention 3-6 has above-mentioned technique effect equally, between each embodiment, diversity is not notable.

Embodiment eight probiotic bacteria of the present invention Fructus Tritici aestivi composite powder shelf-life implants living preparation of lactobacillus stable content is tested

Take the Fructus Tritici aestivi composite powder of the embodiment of the present invention 2 preparation in room temperature 22-25 DEG C, store 3 months, 6 months, 12 months, 24 months, 36 months respectively and measure Lactobacillus plantarum viable bacteria content under ventilation condition, result is table 2 such as:

Table 2: shelf-life implants living preparation of lactobacillus content detection result

Result above shows: the storage stability of Fructus Tritici aestivi composite powder of the present invention is better, environment resistant (temperature, appropriateness, oxygen, illumination, moisture etc.) influence factor's ability is big, shelf-life Lactobacillus plantarum viable bacteria content loss rate maximum (36 months) is 15%, higher than existing like product viable bacteria content, loss rate is low, long shelf-life, reflects that other bioactive ingredients of Fructus Tritici aestivi composite powder has same storage stability simultaneously.

It should be understood that probiotic bacteria Fructus Tritici aestivi composite powder prepared by embodiment of the present invention 3-6 has above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect diversity.

The sensory evaluation test of embodiment nine probiotic bacteria Fructus Tritici aestivi composite powder of the present invention

The Fructus Tritici aestivi composite powder that the embodiment of the present invention 2 is prepared by 24 personnel is invited to judge with the Fructus Tritici aestivi composite powder of commercially available two kinds of similar identical dates of manufacture, sense organ is given a mark, wherein specialty and each 12 of layman, professional is young, middle aged, each 4 of old age, men and women half and half, layman is juvenile, young, middle aged, each 3 of old age, and men and women half and half；Marking includes outward appearance (20 points), quality (25 points), local flavor (30 points), four aspects of mouthfeel (25 points), and marking personnel independently carry out, and are independent of each other, and judges result with guarantee accurate.Having added up judging result, equal score value takes approximation, retains integer, is specifically shown in table 3:

Table 3: sensory evaluation statistical result

Note: show significant difference (P < 0.05) with the different lowercase alphabet of mark in a line, the different capitalization of mark represents difference extremely notable (P < 0.01), indicates same letter and represents difference not notable (P > 0.05).

Result above shows, any one will be substantially better than Commercial wheat bud composite powder to Fructus Tritici aestivi composite powder prepared by the present invention from outward appearance, quality, local flavor and mouthfeel, particularly outward appearance, local flavor and mouthfeel are fabulous, also are adapted for different age group, the consumer of different hierarchy of consumption eats simultaneously.

The embodiment ten probiotic bacteria of the present invention probiotic test of Fructus Tritici aestivi composite powder

The Fructus Tritici aestivi composite powder embodiment of the present invention 2 prepared, preparing into viable count with sterilized water is 2 × 10¹⁰The Lactobacillus plantarum solution of CFU/mL, saves backup in 4 DEG C；

1, the 10mL Lactobacillus plantarum solution kept of going bail for is injected in test tube 1, adopts ten times of stepwise dilutions to 10^-8, take 1mL diluent on flat board, the MRS agar culture medium being cooled to 45 DEG C poured on flat board (sterilizing), shake up rapidly after sterilizing.Will be equipped with the test tube 2 of 10mL Lactobacillus plantarum solution again to be placed in 80-90 DEG C of water-bath and heat 15-25min, take the Lactobacillus plantarum solution after heating and carry out ten times of stepwise dilutions to 10^-8, take 1mL diluent on flat board, the MRS agar culture medium being cooled to 45 DEG C poured into upper at flat board (sterilizing) and shake up rapidly after sterilizing.Finally the flat board before heating and after heating is all cultivated under 35 DEG C of conditions 24h, calculates the quantity before and after heating.

It is shown that Viable detection has reached more than 88%.

2, the resistance test of simulated gastric fluid and intestinal juice: the hydrochloric acid 16.4mL taking 100g/L adds distilled water diluting, make pH value respectively 1.5,2.5 and 3.5, take 100mL dilute hydrochloric acid solution, it is separately added into 1g pepsin, it is made fully to dissolve, obtaining simulated gastric fluid, microporous filter membrane degerming (0.22 μm) is standby.Taking potassium dihydrogen phosphate 6.8g, the 500mL that adds water makes dissolving, regulates pH value to 6.8 with 0.1moL/L sodium hydroxide solution；Separately taking trypsin 10g, the 100mL that adds water makes dissolving, after two liquid mixing, is diluted with water to 1000ml, obtains simulated intestinal fluid, and microporous filter membrane degerming (0.22 μm) is standby.Take the 1mL Lactobacillus plantarum solution kept to join in the simulated gastric fluid of 9mL (i.e. ten times of stepwise dilutions), and fully mix on the oscillator rapidly, be subsequently placed in 30-45 DEG C of quiescent culture 2-4h.Take out culture fluid respectively when 1h, 2h, 3h, 4h and count remaining viable count immediately, comparing with former viable count, it is shown that Viable detection is 98%.Then each 1mL of the culture fluid digesting different time it is taken in simulated gastric fluid, it is inoculated in the simulated intestinal fluid that 9mLpH value is 6.8 respectively, it is placed in 30-45 DEG C of quiescent culture 2-4h, and respectively 0,3,6,24h sampling, measure its viable count, comparing with former viable count, result shows that Viable detection is 99%.

3, simulating the resistance test of cholate: make the solution of 1g/L with pancreatin, and add the Fel Sus domestica salt of 0.8% in the solution, the NaOH adjustment pH with 10% is 8.0, then with 0.45 μm of micro-filtrate membrane filtration degerming.The Lactobacillus plantarum solution inoculum kept by 0.5mL is simulated in cholate to 4.5mL, obtains culture fluid, the viable count of counting remaining after cultivating 24h.By culture fluid in sterile saline ten times of stepwise dilutions to 10^-8, and pour into MRS, it is subsequently placed in 35 DEG C of quiescent culture 24h.Result shows that Viable detection is 99%.

Above result of the test shows, the Lactobacillus plantarum probiotic (thermostability, resistance to pH, bile tolerance) in Fructus Tritici aestivi composite powder of the present invention is relatively strong, is especially suitable for human intestines and stomach's environment, and in simulated gastrointestinal environments, survival rate is big, can be effectively improved gastrointestinal function.

Embodiment 11 probiotic bacteria of the present invention Fructus Tritici aestivi composite powder mouse intestinal performance test

Choosing common Kunming white mice 60, male and female half and half, 18-20g, conventional word is supported.Therefrom random choose 40,9:00 gavage lincomycin hydrochloride 0.2mL every morning (20mg)/only, other as a control group, every day same time gavage equivalent sterile saline, continuous one week, prepare the mouse model of alteration of intestinal flora.Model group mouse diet declines, and dead and phenomenon of significantly suffering from diarrhoea does not occur, arranges soft excrement, and profile normal aqueous divides more, and bedding and padding are moist.By 40 alteration of intestinal flora mices, being randomly divided into 2 groups, one group 20 is only used as treatment group, the Lactobacillus plantarum solution 0.5ml (2 × 10 that every day, gavage was kept¹⁰Cfu/ml)/only, another 20 are only used as natural recovering group, every day same time gavage equivalent sterile saline, continuous two weeks.21 days whole experimental periods, observing growth and the defecation situation of white mice every day, weigh in the 8th, 21 days mices to Fructus Tritici aestivi composite powder treatment group and natural recovering group, calculate each group of weight average rate of increase, result is table 4 such as；Within every 5 days, surveying each group of stool in mice escherichia coli quantity, calculate average, result is table 5 such as.Take stool in mice and be about 0.1g, in aseptic operating platform, add 3 beades (adding 0.5mL diluent with 0.1g excrement sample), dilute and inoculate maconkey agar culture medium, calculate every gram of coliform count wet in just.

Table 4: mice Gain weight

Table 5: the situation of coliform count in stool in mice

Fructus Tritici aestivi composite powder treatment group Mouse Weight average rate of increase (67.96%) is significantly higher than natural recovering group (32.14%)；Feed solution rear intestinal escherichia coli quantity to be remarkably decreased, reduce by 97.07%, it is substantially less than natural recovering group (24.78%), show the rapid field planting in white mice intestinal of the Lactobacillus plantarum in Fructus Tritici aestivi composite powder of the present invention, form dominant microflora, and effectively suppress the growth and breeding of the pathogen such as escherichia coli, and resident time is long, continues, effectively improves intestinal performance.

The impact on immunity of organisms of the embodiment 12 probiotic bacteria Fructus Tritici aestivi composite powder of the present invention

1 experiment purpose

Test (mouse forced swimming) by exercise tolerance, verify the raising immunity of Fructus Tritici aestivi composite powder of the present invention, antifatigue effect.

2 experiment materials and reagent

2.1 for reagent thing:

Commercial wheat bud composite powder (G1)；Commercial wheat bud composite powder (G2)；Fructus Tritici aestivi composite powder (G3-G7) prepared by embodiment of the present invention 2-6.

2.2 reagent:

Liver/muscle glycogen testing cassete, builds up institute of biological products purchased from Nanjing；Concentrated sulphuric acid (AR), Nanjing Chemistry Reagent Co., Ltd.；Normal saline, Shangdong Changfu Jiejing Pharmaceutical Industry Co., Ltd..

3. laboratory animal

ICR mice, ♂, cleaning grade, body weight 18-22g, Ningxia Medical University's comparative medicine center provide, the free diet of mice during experiment.

4. key instrument

Aluminum swimming trunk (50cm × 50cm × 40cm), galvanized wire, low-temperature and high-speed centrifuge: 5804R type, Eppendrof company；Water-bath: DK-S26 type, the grand experimental facilities company limited of upper Nereid；Electronic scale: BS224S type, Sartorius company；Stopwatch, thermometer

5. experiment packet

5.1 dosage packets and given the test agent give the time and at random mice are divided into 8 groups, often group 10,1st group to the 7th group medicine giving G1～G7 respectively, 8th group is blank group, give isopyknic distilled water, the often every average daily gavage of group 1 time, gavage volume is 0.2ml/10g, gives given the test agent continuously 30 days.

5.2 sample preparations the 1st group to the 7th group: weigh 2.25g drug sample, be assigned to 150ml with distilled water；Blank group: distilled water 150ml.

6. experimental technique

After 6.1 swimming with a load attached to the body experiment last administration 30min, putting mice in swimming trunk, the depth of water is no less than 30cm, water temperature 25 ± 1 DEG C, the sheet lead of rat-tail root load 5% body weight, and record mice swimming started to the dead time, as mice swimming time.

After 6.2 mice serum carbamide measure last administration 30min, not swimming with a load attached to the body 90min in the water that temperature is 30 DEG C, eyeball blood sampling 0.5mL (being not added with anticoagulant) is plucked after rest 60min, put 4 DEG C of refrigerator 3h, after hemopexis, the centrifugal 15min of 2000r/min, takes serum and send clinical laboratory of Affiliated Hospital of Ningxia Medical University to detect.

After the mensuration last administration 30min of 6.3 hepatic glycogen, not swimming with a load attached to the body 90min in the water that temperature is 25 ± 1 DEG C, cervical dislocation puts to death mice, clean with normal saline, and with, after filter paper suck dry moisture, accurately weighing liver 100mg, hepatic glycogen detection kit detection Mouse Liver glycogen content.

Blood sampling after the mensuration last administration 30min of 6.4 blood lactase acid, does not then bear a heavy burden and stops after the water went swimming 10min that temperature is 30 DEG C.Lactic acid instrument assay method: respectively before swimming, each blood sampling 20 μ L add in 40 μ L rupture of membranes liquid after rest 20min after swimming, after swimming, fully vibration smudge cells lactic acid instrument measures immediately.(blood lactase acid area under curve=5 × (after front blood lactase acid value+3 × swimming of swimming the blood lactase acid value of 20min after blood lactase acid value+2 × swimming of 0min)

7. observation index walking weight load, blood lactase acid, carbamide, glycogen initial value

8. statistical method experimental data is usedRepresent, adopt t inspection to compare between organizing

9. experimental result

The impact on Mouse Weight of the 9.1 Fructus Tritici aestivi composite powders of the present invention

Each group mice is after giving G1～G9 medicine, before, in, post-weight is shown in shown in following table respectively, and each original body mass organizing mice and weightening finish body weight compare equal no difference of science of statistics (P > 0.05) with matched group, it was shown that G1～G9 medicine is all without obvious toxicity.Experimental result refers to table 6.

The original body mass of table 6 swimming with a load attached to the body experiment mice, mid-term body weight and terminate body weight

The impact on the mice burden swimming time of the 9.2 Fructus Tritici aestivi composite powders of the present invention

After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, the mice burden swimming time can be obviously prolonged, there is significant difference (P < 0.05), Fructus Tritici aestivi composite powder G3～G7 medicine of the present invention compares with blank group, can significantly extend the mice burden swimming time, there is pole significant difference (P < 0.01), and be substantially better than G1～G2 medicine.The results detailed in Table 7.

The impact on the mice burden swimming time of the table 7 Fructus Tritici aestivi composite powder

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

9.3 Fructus Tritici aestivi composite powders of the present invention are on the impact of blood lactase acid before and after mouse movement

After per os gives the Fructus Tritici aestivi composite powder of the mice present invention, blood lactase acid area under curve after mouse movement is compared by Fructus Tritici aestivi composite powder G3～G7 medicine of the present invention with matched group significant difference (P < 0.05), decrease though G1～G2 medicine group Mouse Blood lactic acid area under curve compares with matched group, but and no difference of science of statistics (P > 0.05).Result is in Table 8.

Table 8 Fructus Tritici aestivi composite powder of the present invention is on the impact of blood lactase acid level before and after mouse movement

" * " p < 0.05vs blank；

The impact on Mouse Liver glycogen of the 9.4 Fructus Tritici aestivi composite powders of the present invention

After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, Mouse Liver glycogen content all has obvious rising, there is significant difference (P < 0.05), Fructus Tritici aestivi composite powder G3～G7 medicine of the present invention compares with blank group, Mouse Liver glycogen content all has obvious rising, has pole significant difference (P < 0.01), and is substantially better than G1～G2 medicine.The results detailed in Table 9.

The impact on Mouse Liver glycogen content of the table 9 Fructus Tritici aestivi composite powder of the present invention

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

The impact on mice serum carbamide of the 9.5 Fructus Tritici aestivi composite powders of the present invention

After per os gives mice G1～G7 medicine, G1～G2 medicine group compares with blank group, after mouse movement, serum urea content all has obvious reduction, there is significant difference (P < 0.05), Fructus Tritici aestivi composite powder G3～G7 medicine of the present invention compares with blank group, after mouse movement, serum urea content all has obvious reduction, has pole significant difference (P < 0.01), and is substantially better than G1～G2 medicine.The results detailed in Table 10.

The impact on mice serum urea content of the table 10 Fructus Tritici aestivi composite powder of the present invention

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

10. experiment conclusion

This experiment is tested mainly through mice burden swimming, and the deposit simultaneously detecting Mouse Liver glycogen observes the effect of probiotic bacteria Fructus Tritici aestivi composite powder of the present invention raising immunity, resisting fatigue.Preliminary Results shows below:

1, G3～G7 Fructus Tritici aestivi composite powder of the present invention all can extend the mice burden swimming time (P < 0.01), and effect is substantially better than the Fructus Tritici aestivi composite powder of other G1～G2.

2, biochemistry detection aspect shows, the each dosage group of G3～G7 Fructus Tritici aestivi composite powder of the present invention all can reduce move after lactic acid content produced by glucose anerobic glycolysis in mice serum, compare with matched group and have significant difference (P < 0.05), and although the Fructus Tritici aestivi composite powder of other G1～G2 also can reduce after motion lactic acid content produced by glucose anerobic glycolysis in mice serum, but compare with matched group, no difference of science of statistics (P > 0.05)；

3, each dosage group of G3～G7 Fructus Tritici aestivi composite powder of the present invention all can significantly improve the deposit (P < 0.01) of glycogen in mouse liver, and effect is substantially better than the Fructus Tritici aestivi composite powder of other G1～G2；

4, metabolic arthritis model finds, G3～G7 Fructus Tritici aestivi composite powder of the present invention can significantly reduce the content (P < 0.01) of urea in serum after mice is swum, and effect is substantially better than other G1～G2 Fructus Tritici aestivi composite powder；

11. conclusion

Above-mentioned experiment proves that probiotic bacteria Fructus Tritici aestivi composite powder of the present invention can significantly improve immunity of organisms, improve muscle power and the endurance of mice, the content of urea in serum and lactic acid after reduction mouse movement, and the deposit of glycogen in mouse liver can be significantly improved, contribute to alleviating the fatigue that sports load causes；The time that mice burden swimming to power exhausts can be extended.

Claims

1. a probiotic bacteria Fructus Tritici aestivi composite powder, is mainly prepared by the raw material of following parts by weight: Wheat Sprout Powder 40-60 part, modified dietary fiber 18-28 part, Lactobacillus plantarum powder 6-12 part, ferment powder 5-10 part, oligosaccharide 5-10 part；

Described Lactobacillus plantarum powder is to prepare according to a conventional method with Lactobacillus plantarum CGMCCNO.11763 for starting strain.

2. probiotic bacteria Fructus Tritici aestivi composite powder as claimed in claim 1, it is characterised in that mainly prepared by the raw material of following parts by weight: Wheat Sprout Powder 45-55 part, modified dietary fiber 21-25 part, Lactobacillus plantarum powder 8-10 part, ferment powder 7-9 part, oligosaccharide 6-8 part.

3. probiotic bacteria Fructus Tritici aestivi composite powder as claimed in claim 1, it is characterised in that mainly prepared by the raw material of following parts by weight: Wheat Sprout Powder 50 parts, modified dietary fiber 23 parts, 9 parts of Lactobacillus plantarum powder, ferment powder 8 parts, oligosaccharide 7 parts.

null4. the probiotic bacteria Fructus Tritici aestivi composite powder as described in claim 1-3，It is characterized in that，The preparation method of described Wheat Sprout Powder，Comprise the steps: to be placed on by Semen Tritici aestivi in the ultrasonic washing unit equipped with 0.01-0.03% sodium bicarbonate solution in power 200-400W、Frequency 20-30KHz room temperature ultrasonic cleaning 3-5min，Take out、Drain，In frequency 2450MHz、Power 3000W、Temperature 38-42 DEG C、Thickness of feed layer 2-4cm microwave drying 10-15s,Then put temperature and be 33-36 DEG C、PH value be 6-8 soak in soak 4-6h，Soak contains the compound enzyme that mass percent concentration is 0.15-0.25%，Ventilate once every 15-20min，Ventilation pressure 0.13-0.15MPa，Simultaneously in electric field intensity 10-15kV/cm，Burst length 100-300 μ s,Pulse frequency 60-80Hz condition carries out high voltage pulse electric field processing，Until wheat water content content is 40-45%；Semen Tritici aestivi after immersion drains, and carries out dark germination in growing floor, and dark germination temperature keeps 20-24 DEG C, and the dark germination time is 20-24h, and wheat drying to the moisture after germinateing is 5-8%, low-temperature grinding, crosses 60-80 mesh sieve and namely obtains Wheat Sprout Powder；

5. the probiotic bacteria Fructus Tritici aestivi composite powder as described in claim 1-3, it is characterized in that, described ferment powder preparation method is as follows: adopting the fermentation liquid that pickle fermentation produces discharge in latter stage is raw material, being filtered to remove the big grain materials such as the dish leaf in fermentation liquid and obtain fermented liquid, fermented liquid adopts lyophilization to obtain ferment powder；Cryodesiccated condition is :-30--40 degree pre-freeze 6 hours；Then lyophilizing is until water content is less than 5%.

6. the probiotic bacteria Fructus Tritici aestivi composite powder as described in claim 1-3, it is characterized in that, described oligosaccharide parts by weight consist of oligofructose 40-50 part, Oligomeric maltose 30-40 part, Raffinose 20-40 part, soybean oligo saccharide 16-18 part, oligomeric galactose 10-15 part, oligomeric xylose 10-15 part, oligomeric isomaltose 10-15 part, stachyose 8-12 part.

null7. the probiotic bacteria Fructus Tritici aestivi composite powder as described in claim 1-3，It is characterized in that，Comprise the steps: winter rye、Caulis et Folium Ammopiptanthi Mongolici、Sharkskin collagen protein is respectively washed、Drain，8-10:3-5:2-4 Homogeneous phase mixing in mass ratio，Add the lactic acid moistening 3-8h that pH value is 3.8-4.5 of mixed material quality 0.1-1 times,Pulverize immediately after-18--22 DEG C of freezing 1-2h，Freezing thickness of feed layer 3-5cm，Ground product particle diameter 0.5-3mm,It is subsequently added into the water of ground product quality 10-20 times，It is 3.5-5.5 with breast acid for adjusting pH value，At electric field intensity 25-35kV/cm under room temperature，Burst length 300-500 μ s,High voltage pulse electric field processing 20-30min is carried out when pulse frequency 200-300Hz；Then carry out microwave irradiation and extraction 15-20min when power 150-300W in room temperature, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction；Add the compound enzyme of extracting solution quality 1-2%, in 45-55 DEG C of enzymolysis 30-50min；Enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that namely 0.1-0.3mm obtains protective agent；

Described compound enzyme is cellulase, protease, amylase, pectase, tannase 2-4:1-3:1-3:1-2:1-2 Homogeneous phase mixing in mass ratio.

null8. probiotic bacteria Fructus Tritici aestivi composite powder as claimed in claim 1，It is characterized in that，Prepare protectant preparation method during Lactobacillus plantarum powder，Comprise the steps: winter rye、Caulis et Folium Ammopiptanthi Mongolici、Sharkskin collagen protein is respectively washed、Drain，8-10:3-5:2-4 Homogeneous phase mixing in mass ratio，Add the lactic acid moistening 3-8h that pH value is 3.8-4.5 of mixed material quality 0.1-1 times,Pulverize immediately after-18--22 DEG C of freezing 1-2h，Freezing thickness of feed layer 3-5cm，Ground product particle diameter 0.5-3mm,It is subsequently added into the water of ground product quality 10-20 times，It is 3.5-5.5 with breast acid for adjusting pH value，At electric field intensity 25-35kV/cm under room temperature，Burst length 300-500 μ s,High voltage pulse electric field processing 20-30min is carried out when pulse frequency 200-300Hz；Then carry out microwave irradiation and extraction 15-20min when power 150-300W in room temperature, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction；Add the compound enzyme of extracting solution quality 1-2%, in 45-55 DEG C of enzymolysis 30-50min；Enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that namely 0.1-0.3mm obtains protective agent；

Described microwave irradiation and extraction is that batch (-type) extracts, i.e. microwave exposure 10s, interval 20s.

9. the preparation method of probiotic bacteria Fructus Tritici aestivi composite powder as described in as arbitrary in claim 1-8, it is characterized in that, comprise the steps: according to formula proportion, weigh each raw material, successively ferment powder, Lactobacillus plantarum powder, Wheat Sprout Powder, oligosaccharide are added V-type blending tank Homogeneous phase mixing 25-35min, speed of agitator 20-40r/min, be subsequently adding modified dietary fiber uniformly mixed 12-18min, speed of agitator 40-60r/min, namely sterile filling, sealing, packaging obtain probiotic bacteria Fructus Tritici aestivi composite powder.

10. the preparation method of probiotic bacteria Fructus Tritici aestivi composite powder as claimed in claim 9, it is characterised in that described sterile filling adopts automatic powdery filling machine fill, adopts simultaneously⁶⁰Co irradiation sterilization, irradiation dose 4kGy；Described Wheat Sprout Powder adopts Aluminum-plastic composite bag fill；Ultraviolet or ozonization 20-40min is adopted before described Aluminum-plastic composite bag fill.