CN105794843A - Composition containing lactose high-temperature treated product and Zhongshengmycin - Google Patents
Composition containing lactose high-temperature treated product and Zhongshengmycin Download PDFInfo
- Publication number
- CN105794843A CN105794843A CN201610219987.5A CN201610219987A CN105794843A CN 105794843 A CN105794843 A CN 105794843A CN 201610219987 A CN201610219987 A CN 201610219987A CN 105794843 A CN105794843 A CN 105794843A
- Authority
- CN
- China
- Prior art keywords
- lactose
- zhongshengmycin
- temperature
- treated product
- bacterial wilt
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
Abstract
The invention relates to composition containing a lactose high-temperature treated product and Zhongshengmycin. The lactose high-temperature treated product is obtained in steps as follows: a lactose solution is prepared from lactose and water through mixing in the mass ratio being 3:100, put in a high-temperature and high-pressure sterilizing pot, treated for 30 min at the temperature of 120 DEG and cooled; the mass ratio of the lactose high-temperature treated product to the Zhongshengmycin is 1: (2-3). The invention further provides an application of the composition in preventing and treating bacterial wilt of peanuts. The composition has a remarkable synergistic effect and can remarkably prevent the bacterial wilt of the peanuts, and investment and pollution of chemical agents are reduced.
Description
Technical field
The present invention relates to control of crop disease technical field, a kind of containing lactose high-temperature process product and the combination of Zhongshengmycin
Thing.
Background technology
Zhongshengmycin is a kind of novel agricultural antibiotic that biological control research institute of the Chinese Academy of Agricultural Sciences succeeds in developing, and is by Strepiomyces lavendulae Hainan
The antibiotic that mutation produces, belongs to N-glucosides class alkali water-soluble material.Zhongshengmycin is the protective fungicide that a kind of fungicidal spectrum is wider
Agent, have tag, osmosis, the bacterial disease of crops is had higher activity, but alone to bacterial wilt of peanut
Less effective.
Bacterial wilt of peanut is a kind of bacterial disease based on soil-borne caused by Ralstonia solanacearum, is to endanger in the world
Maximum, distribution are the most extensively, cause damage one of the most serious plant disease, is referred to as " cancer " of plant.At present this disease is prevented
Control main based on chemical means, but use chemical pesticide for a long time in a large number and be easily generated Drug resistance and cause environmental pollution, therefore, as
On the basis of where ensureing bacterial wilt prevention effect, reduce the use of chemical pesticide, reduce environmental pollution, be that bacterial wilt of peanut is prevented
Control major issue to be solved in field.
Summary of the invention
The present invention is directed to the problem that the preventing and treating of above-mentioned bacterial wilt of peanut exists, it is provided that a kind of containing lactose high-temperature process product and Zhongshengmycin
Compositions, improve peanut solanacearum prevention effect.
The present invention realizes above-mentioned purpose and is adopted the technical scheme that: a kind of containing lactose high-temperature process product and the combination of Zhongshengmycin
Thing, described lactose high-temperature process product is to be mixed according to the mass ratio of 3:100 with water by lactose, is configured to lactose solution, so
After lactose solution is put in autoclave sterilization pot, 120 DEG C process 30min, gained after cooling；
Described lactose high-temperature process product is 1:2-3 with the mass ratio of Zhongshengmycin.
The application in terms of preventing and treating bacterial wilt of peanut of the described compositions.
Synergism of the present invention is notable, it is possible to the notable generation preventing bacterial wilt of peanut, reduces input and the pollution of chemical agent.
Detailed description of the invention
Below in conjunction with test data and embodiment, technical scheme and beneficial effect are described further.Of the present invention
Lactose high-temperature process product be that lactose is mixed according to the mass ratio of 3:100 with water, be configured to lactose solution, then by lactose
Solution is put in autoclave sterilization pot, and 120 DEG C process 30min, gained after cooling.The lactose high-temperature process product of gained is regarded
It it is 100% female medicine.
One Toxicity Determination test
(1) medicament is equipped with:
Above-mentioned lactose high-temperature process product is mixed according to 1:1,1:2,1:3,1:4 with Zhongshengmycin, obtains mix preparation
1#-4#。
(2) toxicity test method:
Test uses nephelometry, measures above-mentioned lactose high-temperature process product single dose, Zhongshengmycin single dose, 1#-4# to bacterial wilt of peanut
The virulence of bacterium.Assay method particularly as follows: by lactose high-temperature process product single dose, Zhongshengmycin single dose, 1#-4# respectively according to
The concentration of 200ppm, 100ppm, 50ppm, 25ppm, 12.5ppm injects peanut solanacearum (Peanut bacterial
Wilt) in suspension, by according to concentration inject in peanut solanacearum (Peanut bacterial wilt) suspension, put into 30 ± 1 DEG C
In incubator, cultivate 48 hours, take out and measure its turbidity (making reference with the comparison of injected clear water), calculate it blue or green to Semen arachidis hypogaeae
The EC of rot bacterium50, evaluating the potentiation of pharmacy mix, i.e. CTC≤80 according to co-toxicity coefficient method (CTC) is antagonism
Effect, 80<CTC<120 is summation action, and CTC>=120 are potentiation.
(3) interpretation of result:
| Reagent agent | EC50(ppm) | Actual measurement toxicity index | Theoretical toxicity index | Co-toxicity coefficient |
| Lactose high-temperature process product | 330.88 | 7.87 | ||
| Zhongshengmycin | 26.0 | 100.00 | ||
| 1#(1:1) | 59.44 | 43.82 | 53.94 | 81.2 |
| 2#(1:2) | 25.83 | 100.83 | 69.29 | 145.5 |
| 3#(1:3) | 14.58 | 178.64 | 76.97 | 232.1 |
| 4#(1:4) | 47.26 | 55.11 | 81.57 | 67.6 |
Upper table is each medicament Toxicity Determination result statistical table to bacterial wilt of peanut.As can be known from the above table, described lactose high temperature
When process product is 1:2-3 with Zhongshengmycin mass ratio, synergistic results is notable, and mass ratio is to show potentiation during 1:1, and matter
Amount ratio is for then showing antagonism during 1:4.
Two field medicine effect tests
1. trial crops: select first crop bacterial wilt to occur the most serious Peanut continuous cropping as subjects.
2. test processes and arranges:
3 spraying treatments are set: 200 times of liquid of 3# medicament, 1% Zhongshengmycin 200 times of liquid, clear water.
Above-mentioned spray liquid is sprayed before Semen arachidis hypogaeae onset peak in seedling stage, and Semen arachidis hypogaeae full-bloom stage terminates to carry out bacterial wilt incidence survey, receives
The phase of obtaining carries out determination of yield, using clear water as comparison, calculates the Rectifying efficacies of each process bacterial wilt of peanut, calculates each process simultaneously
Increase of production rate.
3. result of the test such as following table:
| Test processes | Rectifying efficacies (%) to bacterial wilt of peanut | Yield rate of growth (%) |
| 3# medicament | 92.3 | 66.5 |
| Zhongshengmycin | 67.4 | 31.4 |
Finding out from field experiment, using 3# of the present invention to prevent bacterial wilt of peanut, effect is substantially than Zhongshengmycin
Single dose to be got well, and increase of production also becomes apparent from.
Claims (2)
1. one kind contains lactose high-temperature process product and the compositions of Zhongshengmycin, it is characterised in that described lactose high-temperature process is produced
Thing is to be mixed according to the mass ratio of 3:100 with water by lactose, is configured to lactose solution, then lactose solution is put into High Temperature High Pressure
In autoclave, 120 DEG C process 30min, gained after cooling；
Described lactose high-temperature process product is 1:2-3 with the mass ratio of Zhongshengmycin.
Compositions containing lactose high-temperature process product and Zhongshengmycin the most according to claim 1 is in preventing and treating bacterial wilt of peanut side
The application in face.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610219987.5A CN105794843A (en) | 2016-04-08 | 2016-04-08 | Composition containing lactose high-temperature treated product and Zhongshengmycin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610219987.5A CN105794843A (en) | 2016-04-08 | 2016-04-08 | Composition containing lactose high-temperature treated product and Zhongshengmycin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105794843A true CN105794843A (en) | 2016-07-27 |
Family
ID=56459955
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610219987.5A Pending CN105794843A (en) | 2016-04-08 | 2016-04-08 | Composition containing lactose high-temperature treated product and Zhongshengmycin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105794843A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104488930A (en) * | 2014-12-18 | 2015-04-08 | 广西田园生化股份有限公司 | Compound composition containing methanesulphonyl myclobutanil and zhongshengmycin and preparation |
| CN105104027A (en) * | 2015-07-28 | 2015-12-02 | 贵州大学 | Method of preventing tomato bacterial wilt |
-
2016
- 2016-04-08 CN CN201610219987.5A patent/CN105794843A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104488930A (en) * | 2014-12-18 | 2015-04-08 | 广西田园生化股份有限公司 | Compound composition containing methanesulphonyl myclobutanil and zhongshengmycin and preparation |
| CN105104027A (en) * | 2015-07-28 | 2015-12-02 | 贵州大学 | Method of preventing tomato bacterial wilt |
Non-Patent Citations (2)
| Title |
|---|
| 朱程玉: "乳糖在无菌工艺模拟试验中的应用及清洗验证", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
| 河北省滦南县农牧局: "《新编农业实用技术问答》", 30 June 2014 * |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160727 |
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| WD01 | Invention patent application deemed withdrawn after publication |