CN105754837B - Microculture plate and microbial culture system - Google Patents
Microculture plate and microbial culture system Download PDFInfo
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- CN105754837B CN105754837B CN201410781838.9A CN201410781838A CN105754837B CN 105754837 B CN105754837 B CN 105754837B CN 201410781838 A CN201410781838 A CN 201410781838A CN 105754837 B CN105754837 B CN 105754837B
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Abstract
This application discloses a kind of microculture plates,The microculture plate has the top for supplying liquid in the vertical direction and the bottom of discharge liquid,And the first side and the second side on the horizontal direction perpendicular to the vertical direction of connection top and bottom,And including the first film layer and the second film layer,Opposite the first outer surface and the second outer surface provided respectively by first film layer and the second film layer is be provided,Objective microbe to be cultivated is fixed on first outer surface and the second outer surface,First film layer and second film layer have the micropore suitable for penetration by liquid,Wherein,First film layer and the second film layer are sealingly coupled together in the first side and the second side and form hollow inner space,First film layer described in the penetration by liquid being fed to from top in the inner space and the second film layer reach first outer surface and the second outer surface.
Description
Technical field
This application involves a kind of microculture plate and include the microbial culture system of this microculture plate.
Background technology
Photosynthetic microorganism is a kind of microorganism to be lived using light as unique or main energy sources, including microalgae, indigo plant
Bacterium etc. can carry out photosynthetic microorganism containing chlorophyll, and microorganism has important biomolecule utility value.Particularly microalgae
(such as spirulina) rich in protein, can be used as aquatic feed or animal and fowl fodder；Prior, some microalgaes are in specified conditions
Under can largely synthesize secondary metabolites, such as grease, carotenoid, polysaccharide, various bioactivators and trace element,
These substances have high economic value, can be used in the fields such as functional food, food additives, pharmacy.Especially by micro-
Algae large-scale culture obtains rich grease-contained microalgae biomass, by extracting and conversion forms biological liquid fuel (such as：Biological bavin
Oil, aviation kerosine and gasoline), if biological liquid fuel is considered as to solve bioenergy production and the most important way of carbon fixation and emission reduction
One of footpath.
Current microculture mode includes the open or closed microculture sides such as runway pool, duct type
Formula.For a long time, those skilled in the art develop a variety of modifications to these training methods, however, microorganism is trained
Foster productivity per unit and toxigenic capacity etc. are still without breakthrough, and water consumption is big in incubation, high energy consumption, no
Can realize reduces the purpose of energy consumption while microalgae productivity per unit is promoted to greatest extent.
Such as：Raceway pond declines, and biological culture systematic cultivation efficiency is low, and light source utilization rate is insufficient, the homogenize process such as stirring
Energy consumption it is big, condition of culture is difficult to control, and easily generates living contaminants, is taken up a large area, climate influence it is big, simultaneously because from
It so evaporates, the water in cultivating system scatters and disappears seriously, causes culture solution too high in salinity, causes micro algae growth slow, it is difficult to realize high
Density culture, productivity per unit is low, and post processing energy consumption is big, and frond compiling costs is high.
It is higher that duct type microbial culture system establishes cost；Dissolved oxygen inferior separating effect in culture solution, dissolved oxygen accumulation
It can inhibit frond growth to a certain extent；There are a degree of adherent growth, not easy cleaning；More land resource is needed,
Productivity per unit is not high, and operation and maintenance cost are higher.
The either closed microculture side such as the open microculture mode such as runway pool or duct type
Formula, without breakthrough progress in terms of improving productivity per unit and reducing cost, new development in recent years is got up a kind of half-dried
Formula solid state rheology mode, the culture carrier of this kind of training method are typically the material of certain water-retaining property, such as fibrous material,
General mechanism is：Fiber is hung vertically in a certain way, and culture solution supplements from top to bottom, is stored in fibrous inside, in fiber
Surface penetration, infiltration rate and infiltration capacity are common certainly with liquid supply rate etc. in itself by material from the inside for the culture solution of portion's storage
It is fixed.The characteristics of which be entire incubation independent of water as support system, fiber sheet provides certain as microalgae
Culture solution, reduce energy consumption to a certain extent, improve yield, improve space availability ratio, it is but within a certain height, fine
The liquid measure that dimension is discharged in itself for microdisk electrode is uncontrollable in vertical height, when meeting top consumption liquid measure, lower section liquid supply rate
Deficiency cannot meet the growth needs of microalgae；When meeting lower section consumption liquid measure, top liquid measure is excessive, causes the microalgae quilt of outer surface
It washes away.Therefore the control of liquid measure is the difficult point that current half dry type solid state rheology mode can not be broken through.
The content of the invention
The present invention provides a kind of productivity per unit it is high, microbial culture system energy consumption can be reduced and can save
Culture Institute of Micro-biology needs culture solution to be simultaneously capable of the microculture plate of accurate control liquid measure and the microorganism including this culture plate
Culture systems.
For this purpose, according to the one side of the application, a kind of microculture plate is provided, the microculture plate has
Supply in a vertical direction the top of liquid and the bottom of discharge liquid and connection top and bottom perpendicular to described perpendicular
The first side and the second side on the horizontal direction in direction, and including the first film layer and the second film layer, further include respectively by described
Opposite the first outer surface and the second outer surface that first film layer and the second film layer provide, objective microbe to be cultivated are attached to
Be grown on first outer surface and the second outer surface, first film layer and second film layer have that be suitable for liquid saturating
The micropore crossed, wherein, during first film layer and the second film layer are sealingly coupled together in the first side and the second side and are formed
Empty inner space, the first film layer described in the penetration by liquid being fed to from top in the inner space and the second film layer reach
First outer surface and the second outer surface.
According to a possible embodiments, the microculture plate includes the inner space being divided into multiple vertical corridors
Multiple vertical seal lines in road, the multiple vertical gallery are arranged side by side along the horizontal direction.Herein, " vertical " includes
Vertical direction and near vertical direction；" transverse direction " includes horizontal direction and approximate horizontal direction.
According to a possible embodiments, each vertical gallery and each vertical seal line extend to bottom from top.
According to a possible embodiments, the microculture plate further includes cross-section each vertical gallery and each vertical seal line
The multiple horizontal gallerys being separated from each other set, each in the multiple transverse direction gallery and the multiple vertical gallery
In each connection.
According to a possible embodiments, each horizontal gallery extends to microorganism from the first side of microculture plate
The second side of culture plate.
According to a possible embodiments, each horizontal gallery and the vertical gallery section of its tip side are connected by upper-side hole
It is logical.
According to a possible embodiments, the vertical gallery section of each horizontal gallery and its bottom end side is connected by lower-side hole
It is logical.
According to a possible embodiments, the upper-side hole is staggeredly arranged in a vertical direction with the lower-side hole.
According to a possible embodiments, each horizontal gallery and the vertical gallery section of its tip side pass through two upper-side holes
Connection, each horizontal gallery are connected with the vertical gallery section of its bottom end side by a lower-side hole, one lower-side hole and
The distance between each upper-side hole is equal.
According to a possible embodiments, the microculture plate includes the inner space being divided into multiple horizontal corridors
Multiple transverse sealing lines in road, the multiple transverse direction gallery are arranged side by side and are interconnected along the vertical direction.
According to a possible embodiments, odd number transverse sealing line in the multiple transverse sealing line is from inner space
One side extend to the position of the first distance of opposite side away from the inner space, the even number in the multiple transverse sealing line
Transverse sealing line extends to the position of the one side second distance away from the inner space from the opposite side of inner space.
According to a possible embodiments, the microculture plate includes each vertical gallery being divided into along described vertical
Direction is arranged side by side and multiple transverse sealing lines of multiple horizontal gallerys spaced apart from each other, multiple transverse direction gallerys are interconnected.
According to a possible embodiments, for a vertical gallery in the multiple vertical gallery, the vertical corridor
Odd number transverse sealing line in the multiple transverse sealing line in road extends to vertical away from this from the one side of the vertical gallery
The position of the first distance of opposite side of gallery, the even number transverse sealing in the multiple transverse sealing line of the vertical gallery
Line extends to the position of the one side second distance away from the vertical gallery from the opposite side of the vertical gallery.
According to a possible embodiments, for the vertical corridor adjacent with a vertical gallery in the multiple vertical gallery
For road, described in the odd number transverse sealing line from the vertical gallery in the multiple transverse sealing line of the vertical gallery
Opposite side extends to the position of the 3rd distance of the one side away from the vertical gallery, the multiple transverse sealing of the vertical gallery
Even number transverse sealing line in line extends to the opposite side away from the vertical gallery from the one side of the vertical gallery
The position of 4th distance.
According to a possible embodiments, first distance is equal to the second distance.
According to a possible embodiments, the 3rd distance is equal to the 4th distance.
According to a possible embodiments, the microculture plate is included in the inner space edge since the first side
Horizontal direction towards the inclined first slope in top to extend beyond the inner space halfway but not to the first of the second side
Multiple first oblique seal lines of terminal position, so as to form multiple first skew back gallerys and be included in the inner space
In transverse direction to extend beyond the inner space halfway but not towards inclined second slope in top since the second side
To the multiple second oblique seal lines of the second end position of the first side, so as to form multiple second skew back gallerys, the first skew back corridor
Road and the second skew back gallery are interconnected.
According to a possible embodiments, for each vertical gallery, the microculture plate is included from corresponding
First side of vertical gallery starts in transverse direction to extend beyond the corresponding vertical corridor towards the inclined first slope in top
The halfway in road but not to the corresponding vertical gallery the second side first end position the multiple first oblique seal lines, so as to be formed
Multiple first skew back gallerys and including since the second side of the vertical gallery in transverse direction with towards top inclined
Two slopes extend beyond the vertical gallery halfway but not to the first side of the corresponding vertical gallery second end position it is more
A second oblique seal line, so as to form multiple second skew back gallerys, the first skew back gallery and the second skew back gallery are interconnected.
According to a possible embodiments, in a vertical direction in the first end position of each first oblique seal line and each second
Form overlapping region between the second end position of oblique seal line, in the overlapping region, the multiple first oblique seal line and
The multiple second oblique seal line is alternately arranged.
According to a possible embodiments, distance of the first end position away from the second side and the second end position away from
The distance of first side is equal.
According to a possible embodiments, the first slope is equal to second slope.
According to a possible embodiments, it is equipped in the hollow inner space by hydrophobic material or hydrophilic material system
Into interlayer.
According to a possible embodiments, first film layer and second film layer be made material include organic material or
Inorganic material, organic material include polytetrafluoroethylene (PTFE) (PTFE), polysulfones (PS), polypropylene fine (PAN), cellulose acetate (CA),
Polypropylene (PP), chitosan film (CS), compound polyelectrolyte (PEC), ethylene-vinyl alcohol copolymer (EVAL), polyphenylene -1,
2,4 triazines (PPT), inorganic material include ceramic membrane, cellular glass or molecular sieve.
According to a possible embodiments, the micropore, which has, is suitable for penetration by liquid and objective microbe cell is impervious
Aperture size, it is preferable that micropore size size is 0.01-2 microns.
According to a possible embodiments, the liquid is the culture solution that training objective Institute of Micro-biology needs.
According to a possible embodiments, the generation type respectively sealed includes bonding or hot melt.
Present invention also provides a kind of microbial culture systems for including mentioned microorganism culture plate.
According to the microculture plate and microbial culture system of the application, can significantly save needed for microculture
Culture solution and the amount that accurate can control the culture solution of arrival diaphragm plate outer surface, so as to reduce system energy consumption, save toxigenic capacity,
The condition of algae culture preferably is provided, improves productivity per unit；The microculture plate being made of two layers of film layer provides firm
Property and beautiful appearance；It can be effectively prevented from away from culture solution import in the case where the supply opening of liquid feed device is unevenly distributed
Closely locate culture solution it is more, away from culture solution import distant location culture solution lack the phenomenon that；Culture solution can effectively be extended in corridor
Residence time in road makes culture solution penetrate into the outer surface of film layer with having fully more ample times, efficiently reduces culture
Liquid cycle-index saves culture solution and saves to be repeatedly lifted up the energy expenditure of culture solution.
Description of the drawings
Fig. 1 is the schematic diagram according to the microculture plate of the application first preferred embodiment；
Fig. 2 is the schematic diagram according to the microculture plate of the second preferred embodiment of the application；
Fig. 3 is the schematic diagram according to the microculture plate of the application third preferred embodiment；
Fig. 3 a are the schematic diagrames according to the microculture plate of the variant of third preferred embodiment；
Fig. 4 is the schematic diagram according to the microculture plate of the 4th preferred embodiment of the application；
Fig. 5 is the schematic diagram according to the microculture plate of the 5th preferred embodiment of the application；
Fig. 6 is the schematic diagram according to the microculture plate of the 6th preferred embodiment of the application；And
Fig. 7 is the schematic diagram according to the microculture plate of the 7th preferred embodiment of the application；.
Specific embodiment
Microculture plate according to the present invention is suitable for microculture field, includes but are not limited to algae, mushroom
Culture, apply also for the microculture of any other appropriate species.
Herein, term " culture solution " refers to include battalion needed for water and microculture for objective microbe culture
Foster liquid.
Herein, microculture plate is the microculture plate of form membrane, is the carrier of objective microbe culture, one
On the other hand aspect is used to carry for accompanying and growth objective microbe for adhering to and growing the objective microbe being cultured
For the nutrition needed for microculture, i.e. culture solution.
Microculture plate in the application mainly includes two film layers, which forms a plate (or tablet
Bag-shaped or thin layer envelope shape) it is whole, i.e. two film layers are sealably coupled to around formation together sealing and hollow on periphery
Inner space forms the structure type of film-hollow-film.For film layer sheet as microporous barrier, there is micropore on surface, and the size in aperture is set
Into being suitable for culture solution by but preventing objective microbe cell to be cultivated from passing through.
In the case where microculture plate is placed vertically, culture solution is empty from top into the inside between two film layers
Between, while being flowed from top to bottom along the inner surface of film layer, spread, pass through to the outer surface of film layer from the inner surface of film layer
Micropore in film layer is evenly distributed on outer surface, and culture solution is provided to the microbial cell of apposition growth on the outer surface,
Uniformity coefficient of the culture solution provided on the vertical direction of film layer outer surface is controllable, the water supply needed with optimum microalgae
To be optimal.
The material of film layer may be selected to be it is following in one kind：Polytetrafluoroethylene (PTFE) (PTFE), polysulfones (PS), polypropylene are fine
(PAN), cellulose acetate (CA), polypropylene (PP), chitosan film (CS), compound polyelectrolyte (PEC), ethylene-vinyl alcohol
Copolymer (EVAL), polyphenylene -1,2,4 triazines (PPT) and other appropriate inorganic material films, such as ceramic membrane, cellular glass
Or molecular sieve.
Particularly, since polytetrafluoroethylene (PTFE) (PTFE) material has excellent resistance to atmospheric aging, acid-alkali-corrosive-resisting, antioxygen
The series of advantages such as change, high temperature resistant, tensile strength height, so polytetrafluoroethylene (PTFE) (PTFE) material is preferred.With it is other organic
Material is compared, and microculture plate has much longer service life made of polytetrafluoroethylene (PTFE) (PTFE).
Below with reference to the accompanying drawings the microculture plate of the preferred embodiment of the application is described in detail.Technology people in the art
It is to be understood that in order to more clearly illustrate the structure feature of the present invention, attached drawing of the invention is not necessarily drawn to scale member, but may
Part is exaggerated.
Fig. 1 shows the first preferred embodiment according to the application, and microculture plate 10 includes the first opposite film layer
12 and the second film layer (not shown).The first film layer 12 is merely illustrated in Fig. 1, and the second film layer is located at behind the first film layer 12, i.e.,
One side inside page, so the second film layer is invisible in figure.
First film layer 12 and the second film layer are microporous barrier, and material is as described above.The pore size of microporous barrier is set
It can be penetrated into culture solution and microbial cell to be cultivated cannot pass through.
Microculture plate 10 includes opposite both ends in the vertical direction, i.e., for by culture solution supplied to microorganism training
The top 10a of plate 10 is supported, for the culture solution in microculture plate 10 to be discharged to the bottom 10b of microculture plate 10, even
Meet the first opposite side 10c of top 10a and bottom 10b and the second side 10d.
First film layer 12 and the second film layer are sealed connected together in top 10a and in the first side 10c and the second side 10d
And form the hollow inner space flowed through for culture solution.Optionally, the first film layer 12 and the second film layer can be only in the first sides
10c and the second side 10d are connected and sealed.
Microculture plate 10 includes suitable for attachment and grows the first opposite outer surface of objective microbe to be cultivated
12a and the second outer surface.First outer surface 12a is limited by the first film layer 12, and second outer surface is limited by the second film layer, face
Against the inside of page, so invisible in figure.
With reference to figure 1, microculture plate 10 is formed with the gallery 14 of multiple proper widths.Each gallery 14 is vertically
Extension, is arranged side by side in the horizontal direction perpendicular to vertical direction, is separated between two neighboring gallery 14 by seal line 18.
Preferably, each gallery 14 has identical width in the horizontal direction.
Using this structure, the culture solution from culture liquid supply unit is supplied to microculture plate 10 from top
Each gallery 14 adjusts liquid supply speed of the feeding mechanism to each gallery 14, and the dimensional parameters of preferably each gallery 14 are identical, so
It reaches the culture solution of each gallery 14 vertically conformably to flow in gallery 14 with identical flow regime, along the first He
While the inner surface of second film layer flows downward, penetrate through the first and second film layers and reach the of microculture plate 10
One and the second objective microbe on outer surface, objective microbe is made to obtain the culture solution of even amount.
This Corridor Design provides rigidity and beautiful appearance for the microculture plate being made of two layers of film layer.Gallery is also
Play the role of guiding culture solution liquid stream, the liquid stream in each gallery is not interlocked between each other.It is distributed in the supply opening of liquid feed device
In the case of non-uniform, efficiently avoid away from culture solution import closely locate culture solution it is more, away from culture solution import distant location
The phenomenon that culture solution is few.The Corridor Design of appropriate form can effectively extend residence time of the culture solution in gallery, make training
Nutrient solution penetrates into the outer surface of film layer with having fully more ample times, efficiently reduces culture solution cycle-index, saves culture
Liquid and the energy expenditure for saving to be repeatedly lifted up culture solution.
Using gallery structure, each corridor after the width of each gallery 14 is designed such that inside each gallery 14 full of culture solution
Road 14 has expected thickness and culture solution weight.In this way, compared with the culture harden structure of no gallery 14, micro- life can be substantially reduced
Object culture plate 10 is full of the thickness and weight after culture solution.
Using the structure being isolated from each other between this each gallery, culture solution flows in the gallery 14 of microculture plate 10
State is simple, is not influenced each other between each gallery.Particularly, the culture solution circulation of one or more gallerys goes wrong wherein
In the case of, other gallerys are unaffected.The uniformity of the culture solution supply of each gallery 14 can be by cultivating liquid supply unit
Adjusting realize.In addition, this structure also has, culture solution supply rate is fast, can quickly fill with each gallery 14 and correspondingly contract
The advantage of short equipment early-stage preparations time.
Dotted line in Fig. 1 represents the track of stream in the gallery 14.
Fig. 2 shows the second preferred embodiment of the application, and microculture plate reference marker 20 represents.With microorganism
Culture plate 10 is identical, and microculture plate 20 includes the gallery 24 of the same form separated by vertical seal line 28, different
, the vertical gallery 24 of microculture plate 20 is not to be continuously extended to bottom 20b from top 20a, but by it is multiple from
The horizontal gallery 21 that first side 20c extends to the second side 20d is cross-section.
Each horizontal gallery 21 include the upper seal line 21a that is connected with the vertical gallery section of its tip side and with its bottom end side
The lower seal line 21b that vertical gallery section is connected.The vertical gallery section of upper seal line 21a and its tip side is by being separated from each other
First upper-side hole 27 is connected with the second upper-side hole 29, and the vertical gallery section of lower seal line 21b and its bottom end side are perpendicular by being located at
25 hole of lower-side hole connection among straight gallery section.Preferably, 27 and second upper-side hole 29 of the first upper-side hole and the first downside
The distance between hole 25 is equal.
Certainly, the quantity of upper-side hole and the quantity of lower-side hole are not limited to the number of diagram.But it is preferred that upper-side hole and lower-side hole
It is staggeredly arranged in the vertical direction.In addition, each horizontal gallery 21 may not include seal line 21a, but directly with its tip side
Each vertical gallery section connection.In this way, the uppermost and lowermost of each vertical gallery 24 can be substantially guaranteed at along level
The same position in direction, the water-flow equation state in each vertical gallery section is essentially similar, avoids going out in each vertical gallery 24
Situation about now accumulating on the way.In addition, arranged according to actual conditions, according to the difference of gallery, the pore size in each hole in seal line,
Position and width can suitably change.
Using this structure, be supplied to vertical gallery 24 culture solution flow a distance in the vertical direction after, pass through
Two upper-side holes 27 and 29 being located in each gallery 24 on upper seal line 21a and be flowed in horizontal gallery 21, then under
On seal line 21b be located at each gallery 24 in lower-side hole 25 and enter the horizontal gallery bottom end side vertical gallery section in.
That is in horizontal gallery 21, the culture solution in the vertical gallery section of tip side is redistributed to after being converged first
In the vertical gallery section of its bottom end side, by these horizontal gallerys 21, culture solution in each vertical gallery 24 is from top vertical stream
Multiple equilibrium is realized during moving to bottom.Horizontal gallery 21 plays the training in the vertical gallery section for collecting its tip side
Nutrient solution and the effect of redistribution culture solution to the vertical gallery section of its bottom end side.
Illustrative track of stream shown in phantom in Fig. 2, it can be seen that the liquid stream of culture solution is not necessarily from beginning extremely
It is flowed eventually in same gallery.Wherein, vertical seal line 28 and upper and lower seal line 21a and 21b are by by microculture
First film layer 22 of plate 20 and the second film layer are sealingly clamped to together and realize.
As shown in figure 3, being represented according to the microculture plate reference marker 30 of third preferred embodiment, implement with first
Identical or corresponding part increases by 20 to represent with corresponding reference number in example.Microculture plate 30 includes opposite first
32 and second film layer (not shown) of film layer.First film layer 32 and the second film layer are close in top 30a and opposite both sides 30c and 30d
It is enclosed in together and forms inner space.
Microculture plate 30 includes horizontally extending multiple gallerys 34, by close between two neighboring gallery 34
Envelope line 38 separates.
Odd number gallery in the multiple gallery 34 prolongs in the horizontal direction to the second side 30d since the first side 30c
It reaches away from first distances of the second side 30d, the even number gallery in the multiple gallery 34 is from the second side 30d to opposite
The first side 30c extend to away from the first side 30c second distances.Preferably, the first distance is equal to second distance.Equally,
The mobility status of culture solution is as shown in dotted line with the arrow in Fig. 3.
After culture solution is fed to from the first side 30c of top 30a in the inner space of microculture plate 30, arrive first
Up to the horizontal gallery 34 of the top.In the horizontal gallery 34 of the top, culture solution is from the first side 30c initially towards the second side
30d flows.First distance is rationally set so that culture solution is passed through after full of the horizontal gallery by being formed by the described first distance
Indentation, there flow down into the second horizontal gallery 34.Culture solution in the second horizontal gallery 34 is flowed into from the second side 30d towards the
One side 30c flows, and is completely filled with the second horizontal gallery 34 and then flows down into position by the indentation, there that the second distance is formed
In the 3rd gallery 34 below the second horizontal gallery.And so on, culture solution is vertically from the horizontal gallery of the top
34 flow in the horizontal gallery 34 in bottom of bottom 30b, leave microculture plate 30.
Using this structure, residence time is more in the microculture plate 30, i.e. in each horizontal gallery 34 for culture solution
It is long, it is more advantageous to culture solution and fully penetrates the film layer of microculture plate 30 and reach the appearance where objective microbe
Face.It is envisioned that 34 quantity of gallery is more, the width of each gallery 34 is smaller, is more conducive to extend culture solution in gallery 34
Residence time, as shown in Figure 3a.
The microculture plate 40 of the 4th preferred embodiment according to the application, microculture plate is shown in FIG. 4
40 be essentially the combinatory variants of first embodiment and 3rd embodiment.
Specifically, microculture plate 40 includes the multiple vertical gallerys 44 split by vertical seal line 48.It is each vertical
Seal line 48 extends to bottom 40b from top 40a, and the description as described in vertical gallery 44 refers to first embodiment.
Each vertical gallery 44 includes the multiple horizontal gallerys 41 split by horizontal seal line 43, on horizontal gallery 41
Description refer to 3rd embodiment.
Using the microculture plate of this structure, culture solution is more evenly distributed.
Fig. 5 shows the 5th embodiment according to the application, and the 5th embodiment is the variant of the 4th preferred embodiment.
On the one hand microculture plate 50 in Fig. 5 is with 40 difference of microculture plate in Fig. 4：Vertical corridor
Road 54 is more, each vertical gallery 54 in the horizontal direction on width smaller, this, which can be played, extends culture solution in gallery
The effect of residence time in 54.
Microculture plate 50 in Fig. 5 with 40 difference of microculture plate in Fig. 4 further aspect is that：Micro-
In each vertical gallery 44 of biological culture plate 40, culture solution flow regime is specially flow direction, is consistent；And in micro- life
In object culture plate 50, the flowing of culture solution is pair on the seal line between two vertical gallerys 54 in adjacent vertical gallery 54
Claim.
Specifically, in the vertical gallery 54 of odd number in each vertical gallery 54 of microculture plate 50, by this
Vertical gallery 54 is divided into the odd number horizontal seal line 53 from this in multiple horizontal seal lines 53 of multiple horizontal gallerys 51
First its lateral the second side of vertical gallery 54 is extended to away from the 3rd distance of its second side, and the vertical gallery 54 is divided into
Even number horizontal seal line 53 in multiple horizontal seal lines 53 of multiple horizontal gallerys 51 is from the second of the vertical gallery 54
Its lateral first side is extended to away from its 4th distance of the first side.It is preferred that the 3rd distance is equal to the 4th distance.
Meanwhile for the vertical gallery 54 of even number in each vertical gallery 54 of microculture plate 50, by this
Vertical gallery 54 is divided into the odd number horizontal seal line 53 from this in multiple horizontal seal lines 53 of multiple horizontal gallerys 51
Second its lateral first side of vertical gallery 54 is extended to away from its 5th distance of the first side, and the vertical gallery 54 is divided into
Even number horizontal seal line 53 in multiple horizontal seal lines 53 of multiple horizontal gallerys 51 is from the first of the vertical gallery 54
Its lateral the second side is extended to away from the 6th distance of its second side.It is preferred that the 5th distance is equal to the 6th distance.And, it is preferable that the
Three distances are equal to the 5th distance.In this way, in two neighboring vertical gallery 54 culture solution flowing on two vertical gallerys 54 it
Between seal line be symmetrical.
Referring now to Fig. 6 descriptions according to the microculture plate 60 of the 6th preferred embodiment.
Different from the microculture plate shown in Fig. 1-5, microculture plate 60 includes the first film of microporous membrane material
62 and second film layer (invisible in figure) of layer.First film layer 62 and the second film layer are supplying the top 60a and first of culture solution
Side 60c and the second side 60d is tightly connected and forms hollow inner space.By the culture solution of the inner space from bottom
60b outflows are collected.
According to the present embodiment, microculture plate 60 includes two groups of sloped sealing lines being located in the inner space.
First group of seal line 61 is since the first side 60c in the horizontal direction to prolong towards the inclined first slopes of top 60a
It extends more than the inner space halfway but not to the first end position of the second side 60d；Second group of seal line 68 is from the second side
60d starts in the horizontal direction to extend beyond the inner space halfway but not extremely towards inclined second slopes of top 60a
The second end position of first side 60c.In the vertical direction, it is close the first end position of first group of seal line 61 and second group
It seals between the second end position of line 68 and forms overlapping region, in the overlapping region, first group of seal line 61 and described
Second group of seal line 68 is alternately arranged.
Preferably, the distance of the second side 60d of the first end position away from the inner space and the second end
The distance of first side 60c of the position away from the inner space is equal.Additionally preferably, first slope is equal to the second slope.
Culture solution from liquid feed device enters from the top 60a of microculture plate 60 in inner space, into left side
First gallery 64, afterwards, with the increase of culture solution supply, culture solution is from first gallery of left side 64 close to the first side
The bottom of 60c is increasingly towards top 60a along the first seal line 61 and fills first gallery of left side 64, reach first end position it
Afterwards, culture solution overflows.Due to the presence of overlapping region, culture solution is flowed into just in the first gallery 66 of right side.Afterwards, with culture
The increase of liquid, culture solution fill the right side first from the bottom of the close the second side 60d of the first gallery of right side 66 towards top 60a
Gallery 66 overflows after reaching second end position, flows down into the second gallery 64 of left side, and so on.
The advantage of this structure is to overcome the Dead Core Problems inside horizontal gallery, and so-called " dead angle " refers in horizontal gallery
The interior region not being completely filled with since culture solution flowing is too fast.Using this inclined gallery structure, the inclination of each gallery is turned
Corner preferentially stores culture solution, can farthest be kept away by the overlapping degree of the slope and left and right sides gallery that adjust seal line
Exempt from the appearance of Dead Core Problems, while residence time of the culture solution in microculture plate is largely extended.
Sixth embodiment microculture plate and the combining form of first embodiment microculture plate are shown in FIG. 7
.
Microculture plate 70 includes multiple vertical gallerys 74 being arranged side by side, and each the structure in vertical gallery 74 is for example micro-
Described in biological culture plate 60.Which is not described herein again.
All include two film layers as mentioned above for the microculture plate of seven embodiments of the application of each attached drawing description
And the inner space being made of two film layers, although the inner space is before culture solution is supplied in above-mentioned all embodiments
It is kept hollow, but it's not necessary.
Here, applicant also envisages that in the inner space, be specially to provide folder in one or more gallerys wherein
Layer.
Interlayer can be hydrophily interlayer (or being water imbibition interlayer) or hydrophobicity interlayer.
The stream in circulation (water inlet up and down) and horizontal direction that circulation of the culture solution inside interlayer is divided on vertical direction
Logical (diffusion phenomena between interlayer and film).
Hydrophily interlayer：Including non-woven fabrics etc. there are the Fiber Materials of high-hydroscopicity and high-moisture-retention, utilize its high water absorption
Property and high-moisture-retention can flow initial stage in culture solution and carry out substantial amounts of culture solution storage, the characteristic of self-restraint culture solution can be big
The big energy consumption for reducing lifting device (as pumped), such as a saturation amount of storage are enough to supply a few houres even more prolonged outer surface
Micro algae growth, therefore the operation of small number or small flow is enough to complete the promotion to culture solution, and entire system can be greatly reduced
The energy consumption of system.The circulating direction of its internal media is vertically and horizontally to have.The material of the hydrophily interlayer
It can also be polyester (PET).
For hydrophilic (water suction) material in itself, due to the presence of gravity, the driving force spread in horizontal direction is bright
Aobvious to be less than driving force on vertical direction, therefore, culture solution is easier to flow downward, and therefore, liquid supply rate increases, although hydrophily is pressed from both sides
Layer in terms of liquid content for having certain facilitation, but in terms of comprehensive, which exists to diffusion, that is, culture solution of horizontal direction
The aspect that is uniformly distributed of the outer surface of film is no too big contribution, therefore, it may be considered that select appropriate hydrophobicity interlayer.
Hydrophobicity interlayer：The hydrophobicity criterium of material is to have certain water holding capacity, but cannot be completely hydrophobic material.It dredges
The purpose of water is during culture solution flows in the vertical direction, and hydrophobic material is in itself not to the performance state of culture solution
Easily allow culture solution by state, generate certain resistance, it is easier to extend residence time of the culture solution in material internal.For
Into the culture solution inside hydrophobic material, the run into resistance that flows downward overcomes the presence due to gravity to lead as far as possible
Culture solution is caused to be easy to the trend flowed straight down, the stop for longer time that culture solution can be forced inside hydrophobic material, by force
Compel the type of flow and be changed to that horizontal proliferation is preferential by the mode based on vertical, while hydraulic retention of the culture solution inside hydrophobic material
Time can also greatly increase compared with water wetted material, it is ensured that whole system is not required many liquid supply rates to can be completed from folder
Layer carries out the flowing of culture solution to the outer surface of two side forms, therefore liquid supply rate is compared with hydrophily interlayer smaller, whole system
Energy loss smaller, it is more easy to operate.The material of the hydrophobicity interlayer can be polyurethane (PU).
According to the microculture plate and microbial culture system of the application, can significantly save needed for microculture
Culture solution and the amount that accurate can control the culture solution of arrival diaphragm plate outer surface, so as to reduce system energy consumption, save toxigenic capacity,
The condition of algae culture preferably is provided, improves productivity per unit；The microculture plate being made of two film layers provides firm
Property and beautiful appearance；It can be effectively prevented from away from culture solution import in the case where the supply opening of liquid feed device is unevenly distributed
Closely locate culture solution it is more, away from culture solution import distant location culture solution lack the phenomenon that；Culture solution can effectively be extended in corridor
Residence time in road makes culture solution penetrate into the outer surface of film layer with having fully more ample times, efficiently reduces culture
Liquid cycle-index saves culture solution and saves to be repeatedly lifted up the energy expenditure of culture solution.
It only describes the principle of the present invention by way of example above, but is not meant to limit the scope of the invention.On the contrary, this
In the structure that describes can be presented as many other forms.Without departing from the spirit and scope being defined by the below claims
In the case of, those skilled in the art can carry out various alternatives and modifications to above-described embodiment.
Claims (25)
1. a kind of microculture plate, the microculture plate has top and the discharge liquor for supplying liquid in a vertical direction
The bottom of body and the first side and the second side on the horizontal direction perpendicular to the vertical direction of connection top and bottom,
And including the first film layer and the second film layer, further include opposite first provided respectively by first film layer and the second film layer
Outer surface and the second outer surface, objective microbe to be cultivated are attached to and are grown on first outer surface and the second outer surface
On, first film layer and second film layer have the micropore for being suitable for penetration by liquid；
Wherein, first film layer and the second film layer are sealingly coupled together in the first side and the second side and are formed in hollow
Portion space, the first film layer described in the penetration by liquid being fed to from top in the inner space and the second film layer reach described the
One outer surface and the second outer surface,
Wherein, the microculture plate further includes multiple vertical sealings that the inner space is divided into multiple vertical gallerys
Line, the multiple vertical gallery are arranged side by side along the horizontal direction,
Wherein, each vertical gallery and each vertical seal line extend to bottom from top,
Wherein, the microculture plate further includes cross-section each vertical gallery and each vertical seal line is set is separated from each other
It is multiple transverse direction gallerys, it is the multiple transverse direction gallery in each connected with each in the multiple vertical gallery.
2. microculture plate according to claim 1, wherein, each horizontal gallery is from the of microculture plate
One side extends to the second side of microculture plate.
3. microculture plate according to claim 2, wherein, each horizontal gallery and the vertical gallery of its tip side
Section is connected by upper-side hole.
4. microculture plate according to claim 3, wherein, the vertical gallery of each horizontal gallery and its bottom end side
Section is connected by lower-side hole.
5. microculture plate according to claim 4, wherein, the upper-side hole and the lower-side hole are in a vertical direction
It is staggeredly arranged.
6. microculture plate according to claim 5, wherein, each horizontal gallery and the vertical gallery of its tip side
Section is connected by two upper-side holes, each horizontal gallery is connected with the vertical gallery section of its bottom end side by a lower-side hole,
One lower-side hole is equal with the distance between each upper-side hole.
7. according to any microculture plate in claim 1-6, wherein, the microculture plate includes will be each
Vertical gallery is divided into be arranged side by side and multiple transverse sealing lines of multiple horizontal gallerys spaced apart from each other along the vertical direction,
Multiple transverse direction gallerys are interconnected.
8. microculture plate according to claim 7, wherein, for a vertical corridor in the multiple vertical gallery
For road, odd number transverse sealing line in the multiple transverse sealing line of the vertical gallery is from the one side of the vertical gallery
Extend to the position of the first distance of opposite side away from the vertical gallery, in the multiple transverse sealing line of the vertical gallery
Even number transverse sealing line extends to the one side second distance away from the vertical gallery from the opposite side of the vertical gallery
Position.
9. microculture plate according to claim 8, wherein, for vertical with one in the multiple vertical gallery
For the adjacent vertical gallery of gallery, odd number transverse sealing line in the multiple transverse sealing line of the vertical gallery from
The opposite side of the vertical gallery extends to the position of the 3rd distance of the one side away from the vertical gallery, the vertical gallery
Even number transverse sealing line in the multiple transverse sealing line extends to vertical away from this from the one side of the vertical gallery
The position of the 4th distance of the opposite side of gallery.
10. microculture plate according to claim 8, wherein, first distance is equal to the second distance.
11. microculture plate according to claim 9, wherein, the 3rd distance is equal to the 4th distance.
12. according to any microculture plate in claim 1-6, wherein, for each vertical gallery, institute
Stating microculture plate is included since the first side of corresponding vertical gallery in transverse direction with oblique towards top inclined first
Rate extends beyond the halfway of the corresponding vertical gallery but not to the corresponding first end position of the second side of gallery vertically
Multiple first oblique seal lines, so as to form multiple first skew back gallerys and including the edge since the second side of the vertical gallery
Horizontal direction towards inclined second slope in top to extend beyond the vertical gallery halfway but not to the corresponding vertical gallery
The first side second end position the multiple second oblique seal lines, so as to form multiple second skew back gallerys, the first skew back corridor
Road and the second skew back gallery are interconnected.
13. microculture plate according to claim 12, wherein, in a vertical direction the of each first oblique seal line
Overlapping region is formed between the second end position of one terminal position and each second oblique seal line, it is described in the overlapping region
Multiple first oblique seal lines and the multiple second oblique seal line are alternately arranged.
14. microculture plate according to claim 12, wherein, distance of the first end position away from the second side with
The distance of the second end position away from the first side is equal.
15. microculture plate according to claim 12, wherein, the first slope is equal to second slope.
16. according to any microculture plate in claim 1-6, wherein, it is equipped in the hollow inner space
The interlayer made of hydrophobic material or hydrophilic material.
17. according to any microculture plate in claim 1-6, wherein, first film layer and second film
The material that is made of layer includes organic material or inorganic material, and organic material includes polytetrafluoroethylene (PTFE) (PTFE), polysulfones (PS), poly- third
Alkene fine (PAN), cellulose acetate (CA), polypropylene (PP), chitosan film (CS), compound polyelectrolyte (PEC), ethylene-second
Enol copolymer (EVAL), polyphenylene -1,2,4 triazines (PPT), inorganic material include ceramic membrane, cellular glass or molecular sieve.
18. according to any microculture plate in claim 1-6, wherein, the micropore, which has, is suitable for penetration by liquid
And the impervious aperture size of objective microbe cell.
19. microculture plate according to claim 18, wherein, micropore size size is 0.01-2 microns.
20. it is needed according to any microculture plate, the liquid in claim 1-6 for training objective Institute of Micro-biology
Culture solution.
21. according to any microculture plate in claim 1-6, wherein, the generation type respectively sealed includes bonding
Or hot melt.
22. a kind of microculture plate, the microculture plate has top and the discharge for supplying liquid in a vertical direction
The bottom of liquid and the first side and second on the horizontal direction perpendicular to the vertical direction of connection top and bottom
Side, and including the first film layer and the second film layer, further include provided respectively by first film layer and the second film layer it is opposite
First outer surface and the second outer surface, objective microbe to be cultivated are attached to and are grown on outside first outer surface and second
On surface, first film layer and second film layer have the micropore for being suitable for penetration by liquid；
Wherein, first film layer and the second film layer are sealingly coupled together in the first side and the second side and are formed in hollow
Portion space, the first film layer described in the penetration by liquid being fed to from top in the inner space and the second film layer reach described the
One outer surface and the second outer surface,
Wherein, the microculture plate further includes multiple transverse sealings that the inner space is divided into multiple horizontal gallerys
Line, the multiple transverse direction gallery are arranged side by side and are interconnected along the vertical direction.
23. microculture plate according to claim 22, wherein, the odd number in the multiple transverse sealing line is horizontal
The position of the first distance of opposite side away from the inner space, the multiple transverse direction are extended to seal line from the one side of inner space
Even number transverse sealing line in seal line is extended to from the opposite side of inner space away from described in the inner space
The position of one side second distance.
24. a kind of microculture plate, the microculture plate has top and the discharge for supplying liquid in a vertical direction
The bottom of liquid and the first side and second on the horizontal direction perpendicular to the vertical direction of connection top and bottom
Side, and including the first film layer and the second film layer, further include provided respectively by first film layer and the second film layer it is opposite
First outer surface and the second outer surface, objective microbe to be cultivated are attached to and are grown on outside first outer surface and second
On surface, first film layer and second film layer have the micropore for being suitable for penetration by liquid；
Wherein, first film layer and the second film layer are sealingly coupled together in the first side and the second side and are formed in hollow
Portion space, the first film layer described in the penetration by liquid being fed to from top in the inner space and the second film layer reach described the
One outer surface and the second outer surface,
Wherein, the microculture plate is included in the inner space since the first side in transverse direction with towards top
Inclined first slope extends beyond the inner space halfway but not to multiple the first of the first end position of the second side
Oblique seal line, so as to form multiple first skew back gallerys and be included in the inner space since the second side transversely
Direction towards inclined second slope in top to extend beyond the inner space halfway but not to the second end of the first side
Multiple second oblique seal lines of position, so as to form multiple second skew back gallerys, the first skew back gallery and the second skew back gallery phase
It is intercommunicated.
25. a kind of microbial culture system, including according to any microculture plate in claim 1-24.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06190035A (en) * | 1992-10-05 | 1994-07-12 | Minnesota Mining & Mfg Co <3M> | Film oxygen supply equipment |
| CN201418905Y (en) * | 2009-05-07 | 2010-03-10 | 浙江大学 | Fiber mesh stacked bioreactor for artificial liver |
| CN102776120A (en) * | 2012-08-03 | 2012-11-14 | 北京理工大学 | Self-circulating microorganism culture device with oxygen provided by hollow fiber membrane, and culture method |
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2014
- 2014-12-16 CN CN201410781838.9A patent/CN105754837B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06190035A (en) * | 1992-10-05 | 1994-07-12 | Minnesota Mining & Mfg Co <3M> | Film oxygen supply equipment |
| CN201418905Y (en) * | 2009-05-07 | 2010-03-10 | 浙江大学 | Fiber mesh stacked bioreactor for artificial liver |
| CN102776120A (en) * | 2012-08-03 | 2012-11-14 | 北京理工大学 | Self-circulating microorganism culture device with oxygen provided by hollow fiber membrane, and culture method |
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