CN113699031B - Bioreactor for high-density culture of lactobacillus burbulgaricus and use method thereof - Google Patents
Bioreactor for high-density culture of lactobacillus burbulgaricus and use method thereof Download PDFInfo
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Abstract
The invention discloses a bioreactor for high-density culture of lactobacillus broussonensis and a using method thereof, wherein the bioreactor comprises the following steps: the supporting mechanism comprises a base, an adjusting part and a positioning part, the adjusting part and the positioning part are both arranged on the base, the storage mechanism comprises a storage frame, a liquid collecting frame and a power part, the storage frame is arranged on the positioning part, a rotating block is fixedly connected to the center of the bottom of the storage frame, and the liquid collecting frame is arranged inside the storage frame. According to the invention, a proper amount of neutral culture is injected into the mixing frame, the carrying frame and the liquid injection frame, two micro water pumps are started simultaneously, and then a proper amount of neutral culture liquid is injected into the liquid injection frame through the liquid injection pipe, so that the liquid level of the neutral culture liquid in the liquid injection frame can be injected into the liquid conveying pipe through the liquid level pipe after reaching a certain height, and then the neutral culture liquid is effectively injected into the corresponding cache frame through the liquid conveying pipe, and further the neutral culture liquid in the cache frame is slowly injected into the carrying frame through the cotton core of the liquid conveying pipe.
Description
Technical Field
The invention belongs to the technical field of biological reaction equipment, and particularly relates to a bioreactor for high-density culture of lactobacillus burbulgaricus and a using method thereof.
Background
The bioreactor refers to a reaction system in which a liquid phase or a solid phase is inoculated with naturally occurring microorganisms or microorganisms having a specific degradation ability.
However, in the prior art, the existing bioreactor for culturing lactobacillus delbrueckii is inconvenient for actually carrying out internal culture solution and replacement adjustment due to the structural limitation of the existing bioreactor for culturing lactobacillus delbrueckii, the fluctuation of the pH of the internal culture solution is large, and the distribution of nutrient substances is uneven, so that the density of the existing bioreactor for culturing lactobacillus delbrueckii for actually culturing lactobacillus delbrueckii is low, the actual use effect of equipment is poor, meanwhile, the existing bioreactor for culturing lactobacillus delbrueckii cannot uniformly adjust the fusion of reagents in the process of adjusting the internal culture solution, the fusion of the reagents needs to be carried out in a stirring mode, the actual adjustment efficiency of the mode is low, the actual culture effect of lactobacillus delbrueckii is relatively influenced, the actual use effect of the equipment is poor, and meanwhile, the existing bioreactor for culturing lactobacillus delbrueckii cannot carry out the culture of lactobacillus delbrueckii at high density, resulting in more unnecessary resource waste.
Disclosure of Invention
The invention aims to: injecting proper amount of neutral culture into the mixing frame, the object carrying frame and the liquid injection frame, simultaneously starting two micro water pumps, further injecting proper amount of neutral culture liquid into the liquid injection frame through the liquid injection pipe, enabling the liquid level of the neutral culture liquid in the liquid injection frame to reach a certain height, then injecting the neutral culture liquid into the liquid injection pipe through the liquid level pipe, further enabling the neutral culture liquid to be effectively injected into the corresponding cache frame through the liquid injection pipe, further slowly injecting the neutral culture liquid in the cache frame into the object carrying frame through the liquid injection cotton core, simultaneously enabling the neutral culture liquid in the object carrying frame to slowly flow into the connecting frame through the liquid discharge cotton core, further effectively conveying the neutral culture liquid into the lower-layer cache frame through the conveying pipe until the neutral culture liquid flows into the liquid collection frame, and further enabling the neutral culture liquid in the liquid collection frame to be injected into the mixing frame through the first liquid extraction pipe and the first liquid discharge pipe under the action of the two micro water pumps, and then under the detection of test probe body, can pour into the appropriate amount regulating reagent into to mixing frame through the passage, and then effectively mix the frame inside neutral culture solution after adjusting again through second liquid suction pipe and second fluid-discharge tube and come in and go out the liquid frame inside, and then when carrying the inside bacterium film formula of carrying the thing frame and cultivate the lactobacillus broussonensis, carry the inside culture solution nutrient composition of thing frame and pH value and can obtain better maintenance, and then make internal resources can obtain make full use of, effectively improve the culture density that the lactobacillus broussonensis had, make equipment can high-efficiently carry out the realization of function.
The technical scheme adopted by the invention is as follows: a bioreactor for high-density culture of Lactobacillus delbrueckii, comprising:
the supporting mechanism comprises a base, an adjusting part and a positioning part, and the adjusting part and the positioning part are arranged on the base;
the storage mechanism comprises a storage frame, a liquid collecting frame and a power component, the storage frame is arranged on the positioning component, a rotating block is fixedly connected to the center of the bottom of the storage frame, the liquid collecting frame is arranged inside the storage frame, a first liquid suction pipe penetrates through the top of the liquid collecting frame, the edge of the top of the liquid collecting frame is close to one side edge, and the power component is arranged on the storage frame;
the carrying mechanisms are arranged in a plurality of numbers, each carrying mechanism comprises a carrying frame and a conveying part, and the carrying frames and the conveying parts are arranged on the storage mechanism; and
the functional mechanism is arranged on the storage mechanism.
Wherein, adjusting part includes smooth frame and first motor, the equal fixed connection in the inside bottom surface of base of smooth frame and first motor, it is connected with the threaded rod to rotate between the relative interior table wall in smooth frame both sides, the output pot head of first motor is equipped with the interlock gear.
Wherein, threaded rod one end extends to the sliding frame outside, the threaded rod surface is close to one end edge and also is equipped with the interlock gear, two interlock gear engagement, the threaded rod surface is located sliding frame internal thread and is connected with the slider, it is connected with the telescopic link to rotate between the relative interior table wall in slider both sides, telescopic link one end is rotated with the commentaries on classics piece and is connected.
Wherein, locating component is provided with two altogether locating component sets up respectively in base both sides surface, every locating component all includes back shaft, spacer block and locating piece pass through bolt fixed connection on the base, the back shaft rotates and connects on the base, back shaft one end and storing frame fixed connection, locating piece one side surface fixed connection has the backup pad, backup pad one side surface center slides and runs through there is the trace, backup pad top fixedly connected with atmospheric pressure pole, the output fixedly connected with trace of atmospheric pressure pole, trace one side surface and trace one end rotate to be connected.
The utility model discloses a positioning device, including the ejector pin, the ejector pin other end is close to the equal fixedly connected with carriage release lever in both sides edge, two between the carriage release lever surface and be located the inside cover of locating piece and be equipped with first position circle, two be located the inside cover of spacer between the carriage release lever surface and be equipped with the second position circle, first position circle and second position circle are run through in the slip of back shaft one end.
The power component comprises an installation plate and a threaded shaft, the installation plate is fixedly connected to the bottom surface inside the storage frame, the second motor is fixedly connected to the outer surface of one side of the installation plate, the threaded shaft is rotatably connected to the bottom surface inside the storage frame, the outer surface of the threaded shaft is connected with the output end of the second motor through a belt pulley and a belt in a transmission mode, the top end of the threaded shaft penetrates through the bottom of the liquid collecting frame in a sliding mode, and the threaded shaft is connected with the multiple object carrying frames in a threaded mode.
Wherein, every conveying part all includes the cotton core of flowing back, conveyer pipe, connecting frame and buffer memory frame, the equal fixed connection of connecting frame and conveyer pipe is in year thing frame bottom, and the inside intercommunication of conveyer pipe of connecting frame, buffer memory frame fixed connection is in the inside bottom surface of year thing frame, and the inside bottom surface of buffer memory frame runs through there is the cotton core of infusion, the cotton core of flowing back runs through in the inside bottom surface of year thing frame, and the cotton core bottom of flowing back extends to inside the connecting frame.
Wherein, the function mechanism includes sealed lid, sealed lid sets up on the storing frame, sealed caping portion is close to both sides edge and all runs through there is the air duct, sealed caping portion is close to the front side edge and runs through and has annotate the liquid pipe, sealed caping portion is located annotates liquid pipe one side and runs through there are two test probe bodies and passage.
Wherein, table wall fixedly connected with annotates liquid frame in sealed lid one side, annotate liquid frame bottom fixedly connected with transfer line, it has the liquid level pipe to run through to annotate the inside bottom surface of liquid frame, the inside and the inside intercommunication of transfer line of liquid level pipe, the mixed frame of table wall fixedly connected with in the sealed lid opposite side, mix two miniature water pumps of frame top near a side edge fixedly connected with, one of them miniature water pump's input and first liquid suction pipe fixed connection, one of them miniature water pump's output intercommunication is provided with first drain pipe, first drain pipe one end extends to mix inside the frame, another miniature water pump's input and output communicate respectively and are provided with second liquid suction pipe and second drain pipe, second liquid suction pipe and second drain pipe one end extend to mix the frame respectively and annotate inside the liquid frame.
A use method of a bioreactor for culturing lactobacillus burbulgaricus at high density comprises the following steps:
s1, culture solution adjustment: injecting proper amount of neutral culture into the mixing frame, the object carrying frame and the liquid injection frame, simultaneously starting two micro water pumps, further injecting proper amount of neutral culture liquid into the liquid injection frame through the liquid injection pipe, enabling the liquid level of the neutral culture liquid in the liquid injection frame to reach a certain height, then injecting the neutral culture liquid into the liquid injection pipe through the liquid level pipe, further enabling the neutral culture liquid to be injected into the corresponding cache frame through the liquid injection pipe, further slowly injecting the neutral culture liquid in the cache frame into the object carrying frame through the liquid injection cotton core, meanwhile enabling the neutral culture liquid in the object carrying frame to slowly flow into the connecting frame through the liquid discharge cotton core, further conveying the neutral culture liquid into the lower-layer cache frame through the conveying pipe until the neutral culture liquid flows into the liquid collection frame, further enabling the neutral culture liquid in the liquid collection frame to be injected into the mixing frame through the first liquid suction pipe and the first liquid discharge pipe under the action of the two micro water pumps, further under the detection of the detection probe body, a proper amount of regulating reagent can be injected into the mixing frame through the material guide pipe, and the neutral culture solution after the interior of the mixing frame is regulated is delivered into the liquid injection frame again through the second liquid pumping pipe and the second liquid discharge pipe;
s2, state adjustment: through controlling the air pressure rod, and then make the air pressure rod pass through the trace and remove regulation trace service position, and then make the trace adjust first position circle and second position circle service position through the movable rod, and then shift out the locating piece as first position circle, and when the second locating piece did not get into the locating piece yet, control first motor and drive the threaded rod and rotate, and then make threaded rod adjust the slider service position, and then make the telescopic link can cooperate the commentaries on classics piece to drive the storing frame and carry out the deflection of use angle.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
(1) in the invention, a proper amount of neutral culture is injected into the mixing frame, the carrying frame and the liquid injection frame, two micro water pumps are simultaneously started, a proper amount of neutral culture liquid is further injected into the liquid injection frame through the liquid injection pipe, the neutral culture liquid in the liquid injection frame can be injected into the liquid injection pipe through the liquid level pipe after reaching a certain height, the neutral culture liquid is further effectively injected into the corresponding cache frame through the liquid injection pipe, the neutral culture liquid in the cache frame is further slowly injected into the carrying frame through the liquid injection cotton core, the neutral culture liquid in the carrying frame can slowly flow into the connecting frame through the liquid discharge cotton core, and is further effectively conveyed into the lower-layer cache frame through the conveying pipe until the neutral culture liquid flows into the liquid collection frame, and the neutral culture liquid in the liquid collection frame can be injected into the mixing frame through the first liquid suction pipe and the first liquid discharge pipe under the action of the two micro water pumps, and then under the detection of test probe body, can effectively pour into regulating reagent through the passage, and then effectively come in and go out the liquid frame that annotates again with mixed frame inside regulation back neutral culture liquid through second liquid suction pipe and second fluid-discharge tube inside, and then when carrying the inside fungus membrane formula of carrying the thing frame and cultivateing the lactobacillus ragus, carry the inside culture solution nutrient composition of thing frame and pH value and can obtain better maintenance, and then make internal resources can obtain make full use of, effectively improve the cultivation density of lactobacillus ragus, make equipment can high-efficiently carry out the realization that should have the function.
(2) According to the invention, by controlling the air pressure rod, the air pressure rod can effectively adjust the using position of the linkage rod through the movement of the linkage plate, so that the linkage rod can effectively adjust the using positions of the first positioning ring and the second positioning ring through the moving rod, and further when the first positioning ring moves out of the positioning block and the second positioning block does not enter the positioning block yet, the first motor is controlled to drive the threaded rod to rotate, so that the threaded rod can effectively adjust the using position of the sliding block, and further the telescopic rod can effectively drive the storage frame to deflect the using angle in cooperation with the rotating block, so that the flowing speed of the culture solution in the carrying frame is effectively adjusted, further, the device can effectively adjust the self state according to the actual using condition, and further, the actual culture effect of the device is effectively improved.
(3) According to the invention, the second motor is controlled to drive the threaded shaft to rotate, so that the carrying mechanism can be conveniently taken out, the cleaning and maintenance of functional parts inside the equipment are convenient, and the actual use effect of the equipment is effectively improved.
Drawings
FIG. 1 is a front perspective view of the present invention;
FIG. 2 is a rear perspective view of the present invention;
FIG. 3 is a front cross-sectional perspective view of the present invention;
FIG. 4 is an elevational, cross-sectional, expanded perspective view of the support mechanism of the present invention;
FIG. 5 is an enlarged view of portion A of FIG. 4 in accordance with the present invention;
FIG. 6 is an enlarged view of portion B of FIG. 4 in accordance with the present invention;
FIG. 7 is a front cross-sectional perspective view of the storage mechanism of the present invention;
FIG. 8 is an enlarged view of portion C of FIG. 7 in accordance with the present invention;
FIG. 9 is a front cross-sectional perspective view of the carrier mechanism of the present invention;
FIG. 10 is a bottom cross-sectional perspective view of the carrier mechanism of the present invention;
FIG. 11 is an enlarged view of portion D of FIG. 10 in accordance with the present invention;
FIG. 12 is a front cross-sectional expanded perspective view of the functional mechanism of the present invention;
fig. 13 is an enlarged view of portion E of fig. 12 in accordance with the present invention.
The labels in the figure are: 1. a support mechanism; 101. a base; 102. a sliding frame; 103. a threaded rod; 104. a slider; 105. a telescopic rod; 106. a first motor; 107. a linkage gear; 108. a support shaft; 109. a second positioning ring; 110. a first positioning ring; 111. a spacer block; 112. positioning blocks; 113. a support plate; 114. a linkage rod; 115. a travel bar; 116. a pneumatic rod; 117. a linkage plate; 2. a storage mechanism; 201. a storage frame; 202. rotating the block; 203. a threaded shaft; 204. a first liquid suction pipe; 205. a liquid collecting frame; 206. a second motor; 3. A carrying mechanism; 301. a carrying frame; 302. caching a frame; 303. a transfusion cotton core; 304. a connecting frame; 305. a liquid discharge cotton core; 306. a delivery pipe; 4. a functional mechanism; 401. a sealing cover; 402. an air duct; 403. a mixing frame; 404. a detection probe body; 405. a material guide pipe; 406. a micro water pump; 407. liquid injection frame; 408. a transfusion tube; 409. a liquid level tube; 410. and a liquid injection pipe.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example one, with reference to fig. 1-13: a bioreactor for high-density culture of Lactobacillus delbrueckii, comprising: the supporting mechanism 1 comprises a base 101, an adjusting part and a positioning part, the base 101 is arranged to provide an installation foundation for arranging other functional parts of equipment, the adjusting part and the positioning part are arranged on the base 101, the storage mechanism 2 is provided with a storage frame 201, a liquid collecting frame 205 and a power part, the storage frame 201 is arranged to facilitate installation and arrangement of the other functional parts of the equipment, effective protection of internal functional parts can be effectively performed, the liquid collecting frame 205 is arranged to effectively perform temporary storage of culture liquid, the storage frame 201 is arranged on the positioning part, a rotating block 202 is fixedly connected to the center of the bottom of the storage frame 201, the telescopic rod 105 can effectively rotate to adjust the actual use angle of the storage frame 201 due to the arrangement of the rotating block 202, the liquid collecting frame 205 is arranged in the storage frame 201, a first liquid pumping pipe 204 penetrates through the top of the liquid collecting frame 205 and is close to one side edge, first liquid suction pipe 204 establish the cooperation first liquid discharge pipe make miniature pump 406 can effectively be with album inside culture solution of liquid frame 205 reinjection mixing frame 403 inside, power component sets up on storing frame 201, it constructs 3 to carry thing and is provided with a plurality ofly altogether, every is carried thing mechanism 3 and all includes year thing frame 301 and delivery unit, the establishment of carrying thing frame 301 conveniently carries out the installation setting of other functional unit of equipment, the effective cultivation of broken cloth lactobacillus can effectively be carried out simultaneously, it all sets up on storing mechanism 2 with delivery unit to carry thing frame 301, and functional mechanism 4, functional mechanism 4 sets up on storing mechanism 2.
Referring to FIGS. 3-13: the adjusting component comprises a sliding frame 102 and a first motor 106, the sliding frame 102 is convenient to install and set other functional components of the device, the first motor 106 is used for providing power for rotation of a threaded rod 103, the sliding frame 102 and the first motor 106 are both fixedly connected to the bottom surface inside a base 101, the threaded rod 103 is rotatably connected between the opposite inner surface walls of the two sides of the sliding frame 102, the use position of the sliding block 104 can be effectively adjusted by setting the threaded rod 103, an output end of the first motor 106 is sleeved with a linkage gear 107, the first motor 106 can effectively drive the threaded rod 103 to rotate by setting the linkage gear 107, one end of the threaded rod 103 extends to the outside of the sliding frame 102, the outer surface of the threaded rod 103 close to one end edge is also sleeved with the linkage gear 107, the two linkage gears 107 are meshed, the outer surface of the threaded rod 103 is located inside the sliding frame 102 and is in threaded connection with the sliding block 104, and the setting of the sliding block 104 is convenient to install and set the telescopic rod 105, meanwhile, the actual use position of the telescopic rod 105 can be effectively adjusted, the telescopic rod 105 is rotatably connected between the opposite inner surface walls of the two sides of the sliding block 104, the telescopic rod 105 can be effectively matched with the rotating block 202 to effectively drive the storage frame 201 to perform angle rotation adjustment, one end of the telescopic rod 105 is rotatably connected with the rotating block 202, two positioning parts are arranged, the two positioning parts are respectively arranged on the outer surfaces of the two sides of the base 101, each positioning part comprises a supporting shaft 108, a spacing block 111 and a positioning block 112, the supporting shaft 108 is conveniently arranged to be matched with the positioning block 112, the first positioning ring 110 and the second positioning ring 109, the rotation angle of the storage frame 201 can be effectively fixed and limited, the spacing block 111 and the positioning block 112 are fixedly connected on the base 101 through bolts, the supporting shaft 108 is rotatably connected on the base 101, one end of the supporting shaft 108 is fixedly connected with the storage frame 201, the outer surface of one side of the positioning block 112 is fixedly connected with a supporting plate 113, the supporting plate 113 is convenient to install and arrange a linkage rod 114, the linkage rod 114 penetrates through the center of the outer surface of one side of the supporting plate 113 in a sliding manner, the arrangement of the linkage rod 114 is matched with a moving rod 115 to effectively adjust the use positions of the first positioning ring 110 and the second positioning ring 109, an air pressure rod 116 is fixedly connected to the top of the supporting plate 113, the arrangement of the air pressure rod 116 is matched with a linkage plate 117 to effectively adjust the actual use position of the linkage rod 114, the output end of the air pressure rod 116 is fixedly connected with a linkage plate 117, the outer surface of one side of the linkage plate 117 is rotatably connected with one end of the linkage rod 114, the other end of the linkage rod 114 is fixedly connected with moving rods 115 close to the edges of the two sides, the arrangement of the moving rod 115 is convenient to install and arrange the first positioning ring 110 and the second positioning ring 109, the first positioning ring 110 is sleeved between the outer surfaces of the two moving rods 115 and positioned inside the positioning block 112, the first positioning ring 110 and the second positioning ring 109 are matched with the positioning block 112 to effectively fix the rotation angle of the supporting shaft 108, the second positioning ring 109 is sleeved inside the spacer 111 between the outer surfaces of the two moving rods 115, one end of the supporting shaft 108 penetrates through the first positioning ring 110 and the second positioning ring 109 in a sliding manner, the power part comprises a mounting plate and a threaded shaft 203, the threaded shaft 203 is convenient to mount and arrange other functional parts of the equipment, the mounting plate is fixedly connected to the inner bottom surface of the storage frame 201, the outer surface of one side of the mounting plate is fixedly connected with a second motor 206, the power required by the rotation of the threaded shaft 203 is provided by the arrangement of the second motor 206, the threaded shaft 203 is rotatably connected to the inner bottom surface of the storage frame 201, the outer surface of the threaded shaft 203 is in transmission connection with the output end of the second motor 206 through a belt pulley and a belt, the top end of the threaded shaft 203 penetrates through the bottom of the liquid collection frame 205 in a sliding manner, and the threaded shaft 203 is in threaded connection with the plurality of the object carrying frames 301, each conveying component comprises a liquid drainage cotton core 305, a conveying pipe 306, a connecting frame 304 and a buffer frame 302, the liquid drainage cotton core 305 is arranged to enable culture fluid in the object carrying frame 301 to be effectively injected into the connecting frame 304, the conveying pipe 306 is arranged to enable the culture fluid in the connecting frame 304 to be effectively injected into the lower-layer buffer frame 302 or the liquid collecting frame 205, the connecting frame 304 is arranged to be matched with the buffer frame 302 to effectively convey the culture fluid, the connecting frame 304 and the conveying pipe 306 are fixedly connected to the bottom of the object carrying frame 301, the inside of the connecting frame 304 is communicated with the inside of the conveying pipe 306, the buffer frame 302 is fixedly connected to the bottom surface in the object carrying frame 301, the bottom surface in the buffer frame 302 is penetrated with the liquid drainage cotton core 303, the liquid drainage cotton core 303 is arranged to enable the culture fluid in the buffer frame 302 to be effectively injected into the object carrying frame 301, the liquid drainage cotton core 305 is penetrated through the bottom surface in the object carrying frame 301, and the bottom end of the liquid drainage cotton core 305 extends into the connecting frame 304, the functional mechanism 4 comprises a sealing cover 401, the sealing cover 401 is convenient to install and set other functional components of the device, meanwhile, the sealing cover can be matched with the storage frame 201 to effectively perform safety protection on internal functional components, the sealing cover 401 is arranged on the storage frame 201, air guide pipes 402 penetrate through the top of the sealing cover 401 close to the edges of two sides, the air guide pipes 402 can effectively adjust the gas content in the device, further, the actual culture effect of lactobacillus burkei is effectively improved, the actual culture density of lactobacillus burkei is improved, a liquid injection pipe 410 penetrates through the top of the sealing cover 401 close to the edge of the front side, a proper amount of neutral culture liquid can be effectively injected into the liquid injection frame 407 by the arrangement of the liquid injection pipe 410, two detection probe bodies 404 and a material guide pipe 405 penetrate through the top of the liquid injection pipe 410, the arrangement of the detection probe bodies 404 can effectively detect the PH value and the nutrient content of the culture liquid in the moderating frame, further, the culture solution can be effectively regulated, the material guide pipe 405 can effectively inject a proper amount of regulating reagent into the mixing frame 403, the inner surface wall of one side of the sealing cover 401 is fixedly connected with the liquid injection frame 407, the liquid injection frame 407 can effectively temporarily store the culture solution, the bottom of the liquid injection frame 407 is fixedly connected with the liquid conveying pipe 408, the liquid conveying pipe 408 is arranged to enable the culture solution in the liquid injection frame 407 to be effectively injected into the cache frame 302, the bottom surface of the inner part of the liquid injection frame 407 is penetrated with the liquid level pipe 409, the arrangement of the liquid level pipe 409 can effectively ensure that a certain amount of culture solution is reserved in the liquid injection frame 407, the inner part of the liquid level pipe 409 is communicated with the inner part of the liquid conveying pipe 408, the inner surface wall of the other side of the sealing cover 401 is fixedly connected with the mixing frame 403, the arrangement of the mixing frame 403 is convenient for installing other functional components of the equipment, the two micro water pumps 406 are fixedly connected to the top of the mixing frame 403 near the edge of one side, the miniature water pumps 406 are arranged to effectively move and adjust the flow of the culture solution, the input end of one miniature water pump 406 is fixedly connected with the first liquid pumping pipe 204, the output end of one miniature water pump 406 is communicated with a first liquid discharging pipe, one end of the first liquid discharging pipe extends into the mixing frame 403, the input end and the output end of the other miniature water pump 406 are respectively communicated with a second liquid pumping pipe and a second liquid discharging pipe, the arrangement of the second liquid pumping pipe and the second liquid discharging pipe enables the miniature water pump 406 to effectively inject the culture solution in the mixing frame 403 into the liquid injection frame 407, one end of the second liquid pumping pipe and one end of the second liquid discharging pipe extend into the mixing frame 403 and the liquid injection frame 407 respectively, and the arrangement of the second liquid pumping pipe and the second liquid discharging pipe enables the miniature water pump 406 to effectively inject the culture solution in the mixing frame 403 into the liquid injection frame 407.
A use method of a bioreactor for culturing lactobacillus burbulgaricus at high density comprises the following steps: step one, culture solution adjustment: injecting a proper amount of neutral culture into the mixing frame 403, the object carrying frame 301 and the liquid injecting frame 407, simultaneously starting two micro water pumps 406, further injecting a proper amount of neutral culture liquid into the liquid injecting frame 407 through the liquid injecting pipe 410, so that the liquid level of the neutral culture liquid in the liquid injecting frame 407 reaches a certain height and then can be injected into the liquid injecting pipe 408 through the liquid level pipe 409, further enabling the neutral culture liquid to be effectively injected into the corresponding buffer frame 302 through the liquid injecting pipe 408, further slowly injecting the neutral culture liquid in the buffer frame 302 into the object carrying frame 301 through the liquid injecting cotton core 303, simultaneously enabling the neutral culture liquid in the object carrying frame 301 to slowly flow into the connecting frame 304 through the liquid injecting cotton core 305, further effectively conveying the neutral culture liquid into the lower buffer frame 302 through the conveying pipe 306 until the neutral culture liquid flows into the liquid collecting frame 205, and further enabling the neutral culture liquid in the liquid collecting frame 205 to be injected into the mixing frame 403 through the first liquid extracting pipe 204 and the first liquid injecting pipe under the action of the two micro water pumps 406, and then under the detection of test probe body 404, can inject right amount regulating reagent to mixing frame 403 through passage 405, and then effectively mix the inside neutral culture solution of adjusting back of frame 403 and come in and go out again and annotate inside liquid frame 407 through second liquid suction pipe and second fluid-discharge tube, and then when carrying the inside fungus membrane formula cultivation broussonetia papyrifera that carries thing frame 301, carry the inside culture solution nutrient composition of thing frame 301 and pH value and can obtain better maintenance, and then make inside resource can obtain make full use of, effectively improve the culture density of broussonetia papyrifera, make equipment can high-efficient the realization that should have the function, step two, state regulation: by controlling the pneumatic rod 116, the pneumatic rod 116 can effectively adjust the using position of the linkage rod 114 through the linkage plate 117, and the linkage rod 114 can effectively adjust the using positions of the first positioning ring 110 and the second positioning ring 109 through the moving rod 115, so that when the first positioning ring 110 moves out of the positioning block 112 and the second positioning block 112 does not yet enter the positioning block 112, the first motor 106 is controlled to drive the threaded rod 103 to rotate, and further the threaded rod 103 can effectively adjust the using position of the sliding block 104, and further the telescopic rod 105 can cooperate with the rotating block 202 to effectively drive the storage frame 201 to deflect at the using angle, and further the flowing speed of the culture solution inside the carrying frame 301 is effectively adjusted, so that the device can effectively adjust the self-state according to the actual using condition, and further effectively improve the actual culturing effect of the device, and the threaded shaft 203 is driven to rotate by controlling the second motor 206, and then can be convenient take out carrying thing mechanism 3, and then conveniently carry out the clean maintenance of the inside functional unit of equipment, and then effectively improve equipment in-service use effect.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (7)
1. A bioreactor for high-density culture of Lactobacillus delbrueckii, comprising:
the supporting mechanism (1) comprises a base (101), an adjusting part and a positioning part, wherein the adjusting part and the positioning part are both arranged on the base (101);
the storage mechanism (2) comprises a storage frame (201), a liquid collecting frame (205) and a power component, the storage frame (201) is arranged on the positioning component, a rotating block (202) is fixedly connected to the center of the bottom of the storage frame (201), the liquid collecting frame (205) is arranged inside the storage frame (201), a first liquid pumping pipe (204) penetrates through the top of the liquid collecting frame (205) close to the edge of one side, and the power component is arranged on the storage frame (201);
the carrying mechanisms (3) are arranged in a plurality of numbers, each carrying mechanism (3) comprises a carrying frame (301) and a conveying component, and the carrying frames (301) and the conveying components are arranged on the storage mechanism (2); the functional mechanism (4) is arranged on the storage mechanism (2);
each conveying component comprises a liquid drainage cotton core (305), a conveying pipe (306), a connecting frame (304) and a buffer frame (302), the connecting frame (304) and the conveying pipe (306) are fixedly connected to the bottom of the object carrying frame (301), the inside of the connecting frame (304) is communicated with the inside of the conveying pipe (306), the buffer frame (302) is fixedly connected to the bottom surface of the inside of the object carrying frame (301), the liquid drainage cotton core (303) penetrates through the bottom surface of the inside of the buffer frame (302), the liquid drainage cotton core (305) penetrates through the bottom surface of the inside of the object carrying frame (301), and the bottom end of the liquid drainage cotton core (305) extends to the inside of the connecting frame (304);
the functional mechanism (4) comprises a sealing cover (401), the sealing cover (401) is arranged on the storage frame (201), air guide pipes (402) penetrate through the top of the sealing cover (401) close to the edges of two sides, a liquid injection pipe (410) penetrates through the top of the sealing cover (401) close to the edge of the front side, and two detection probe bodies (404) and a material guide pipe (405) penetrate through the top of the sealing cover (401) at one side of the liquid injection pipe (410);
the liquid injection frame (407) is fixedly connected to the inner surface wall of one side of the sealing cover (401), the infusion tube (408) is fixedly connected to the bottom of the liquid injection frame (407), the liquid level tube (409) penetrates through the bottom surface of the inner portion of the liquid injection frame (407), the inner portion of the liquid level tube (409) is communicated with the inner portion of the infusion tube (408), the inner surface wall of the other side of the sealing cover (401) is fixedly connected with the mixing frame (403), the top of the mixing frame (403) is close to one side edge of the mixing frame and is fixedly connected with two micro water pumps (406), the input end of one micro water pump (406) is fixedly connected with the first liquid extraction tube (204), the output end of one micro water pump (406) is communicated with the first liquid extraction tube, one end of the first liquid extraction tube extends to the inner portion of the mixing frame (403), and the input end and the output end of the other micro water pump (406) are respectively communicated with the second liquid extraction tube and the second liquid extraction tube, one end of the second liquid extracting pipe and one end of the second liquid discharging pipe extend to the interior of the mixing frame (403) and the interior of the liquid injecting frame (407) respectively.
2. The bioreactor for high-density culture of lactobacillus broussonensis according to claim 1, wherein: the adjusting part comprises a sliding frame (102) and a first motor (106), the sliding frame (102) and the first motor (106) are fixedly connected to the inner bottom surface of the base (101), a threaded rod (103) is rotatably connected between the two opposite inner surface walls of the two sides of the sliding frame (102), and an output end of the first motor (106) is sleeved with an interlocking gear (107).
3. The bioreactor for high-density culture of lactobacillus broussonensis according to claim 2, wherein: threaded rod (103) one end extends to smooth frame (102) outside, threaded rod (103) surface is close to one end edge and also is equipped with interlock gear (107), two interlock gear (107) meshing, threaded rod (103) surface is located smooth frame (102) internal thread and is connected with slider (104), it is connected with telescopic link (105) to rotate between the relative interior table wall in slider (104) both sides, telescopic link (105) one end and commentaries on classics piece (202) rotate to be connected.
4. The bioreactor for high-density culture of lactobacillus broussonensis according to claim 3, wherein: the number of the positioning parts is two, the two positioning parts are respectively arranged on the outer surfaces of the two sides of the base (101), each positioning part comprises a supporting shaft (108), a spacing block (111) and a positioning block (112), the spacing block (111) and the positioning block (112) are fixedly connected to the base (101) through bolts, the supporting shaft (108) is rotatably connected to the base (101), one end of the supporting shaft (108) is fixedly connected with the storage frame (201), a supporting plate (113) is fixedly connected with the outer surface of one side of the positioning block (112), a linkage rod (114) is penetrated through the center of the outer surface of one side of the supporting plate (113) in a sliding way, the top of the supporting plate (113) is fixedly connected with an air pressure rod (116), the output end of the air pressure rod (116) is fixedly connected with a linkage plate (117), the outer surface of one side of the linkage plate (117) is rotatably connected with one end of the linkage rod (114).
5. The bioreactor for high-density culture of lactobacillus burbuensis as claimed in claim 4, wherein: the utility model discloses a location structure, including the utility model discloses a sensor, including the sensor, the sensor is connected with the sensor, the even fixedly connected with carriage release lever (115) of trace (114) other end is close to both sides edge, two between carriage release lever (115) surface and be located inside cover of locating piece (112) and be equipped with first position circle (110), two between carriage release lever (115) surface be located the inside cover of spacer block (111) and be equipped with second position circle (109), back shaft (108) one end slides and runs through first position circle (110) and second position circle (109).
6. The bioreactor for high-density culture of lactobacillus broussonensis according to claim 5, wherein: the power part includes mounting panel and threaded shaft (203), mounting panel fixed connection is in the inside bottom surface of storing frame (201), and mounting panel one side surface fixed connection has second motor (206), threaded shaft (203) rotate to be connected in the inside bottom surface of storing frame (201), and is connected through belt pulley and belt drive between the output of threaded shaft (203) surface and second motor (206), threaded shaft (203) top slides and runs through album liquid frame (205) bottom, threaded shaft (203) and a plurality of year equal threaded connection of thing frame (301).
7. A method for using a bioreactor for high-density culture of Lactobacillus delbrueckii, which is applied to the bioreactor for high-density culture of Lactobacillus delbrueckii according to any of claims 1 to 6, comprising the following steps:
s1, culture solution adjustment: injecting a proper amount of neutral culture into the mixing frame (403), the carrying frame (301) and the liquid injection frame (407), simultaneously starting two micro water pumps (406), further injecting a proper amount of neutral culture liquid into the liquid injection frame (407) through a liquid injection pipe (410), enabling the liquid level of the neutral culture liquid in the liquid injection frame (407) to reach a certain height, then injecting the neutral culture liquid into the liquid injection pipe (408) through a liquid level pipe (409), further enabling the neutral culture liquid to be injected into the corresponding cache frame (302) through the liquid injection pipe (408), further slowly injecting the neutral culture liquid in the cache frame (302) into the carrying frame (301) through an infusion cotton core (303), meanwhile enabling the neutral culture liquid in the carrying frame (301) to slowly flow into the connecting frame (304) through a cotton core (305), further conveying the neutral culture liquid into the lower-layer cache frame (302) through a liquid discharge pipe (306), and enabling the neutral culture liquid to flow into the liquid collection frame (205), under the action of two micro water pumps (406), neutral culture fluid in the liquid collection frame (205) can be injected into the mixing frame (403) through the first liquid extraction pipe (204) and the first liquid discharge pipe, under the detection of the detection probe body (404), a proper amount of regulating reagent can be injected into the mixing frame (403) through the material guide pipe (405), and the neutral culture fluid after the regulation in the mixing frame (403) is delivered into the liquid injection frame (407) again through the second liquid extraction pipe and the second liquid discharge pipe;
s2, state adjustment: through controlling pneumatic rod (116), and then make pneumatic rod (116) move through trace board (117) and adjust trace rod (114) position of use, and then make trace rod (114) adjust first position circle (110) and second position circle (109) position of use through movable rod (115), and then shift out locating piece (112) as first position circle (110), and when second locating piece (112) had not got into locating piece (112), control first motor (106) and drive threaded rod (103) and rotate, and then make threaded rod (103) adjust the slider (104) position of use, and then make telescopic link (105) can cooperate commentaries on classics piece (202) to drive storing frame (201) and carry out the deflection of use angle.
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