CN105747271B - A kind of preparation method of low albumen tobacco extract for novel tobacco - Google Patents
A kind of preparation method of low albumen tobacco extract for novel tobacco Download PDFInfo
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- CN105747271B CN105747271B CN201610111815.6A CN201610111815A CN105747271B CN 105747271 B CN105747271 B CN 105747271B CN 201610111815 A CN201610111815 A CN 201610111815A CN 105747271 B CN105747271 B CN 105747271B
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- tobacco
- fraction
- extract
- concentrate
- flue
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- 241000208125 Nicotiana Species 0.000 title claims abstract description 87
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 80
- 239000000284 extract Substances 0.000 title claims abstract description 39
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 239000012141 concentrate Substances 0.000 claims abstract description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 13
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 12
- 210000002196 fr. b Anatomy 0.000 claims abstract description 11
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims abstract description 7
- 239000002904 solvent Substances 0.000 claims abstract description 7
- 239000000706 filtrate Substances 0.000 claims abstract description 6
- 238000001914 filtration Methods 0.000 claims abstract description 6
- 238000010992 reflux Methods 0.000 claims abstract description 6
- 238000005292 vacuum distillation Methods 0.000 claims abstract description 6
- 108090000145 Bacillolysin Proteins 0.000 claims abstract description 4
- 102000035092 Neutral proteases Human genes 0.000 claims abstract description 4
- 108091005507 Neutral proteases Proteins 0.000 claims abstract description 4
- 235000019504 cigarettes Nutrition 0.000 claims description 21
- 235000019505 tobacco product Nutrition 0.000 claims description 8
- 210000003918 fraction a Anatomy 0.000 claims description 5
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical class CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052799 carbon Inorganic materials 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 6
- 238000010438 heat treatment Methods 0.000 abstract description 5
- 102000004169 proteins and genes Human genes 0.000 abstract description 5
- 108090000623 proteins and genes Proteins 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 abstract description 3
- 238000000605 extraction Methods 0.000 abstract description 2
- 239000008236 heating water Substances 0.000 abstract description 2
- 230000008569 process Effects 0.000 abstract description 2
- 238000010298 pulverizing process Methods 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 239000003205 fragrance Substances 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 239000008896 Opium Substances 0.000 description 3
- 229960001027 opium Drugs 0.000 description 3
- 239000002304 perfume Substances 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 101710138460 Leaf protein Proteins 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 235000019506 cigar Nutrition 0.000 description 1
- 239000011285 coke tar Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000003571 electronic cigarette Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000002798 polar solvent Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
- A24B15/241—Extraction of specific substances
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/10—Chemical features of tobacco products or tobacco substitutes
- A24B15/16—Chemical features of tobacco products or tobacco substitutes of tobacco substitutes
Abstract
The present invention provides a kind of preparation method and application of low albumen tobacco extract for novel tobacco, using tobacco as raw material, through pulverization process, according to feed liquid mass ratio 1:4~1:8 ratio adds 60%~95% ethanol of mass fraction, 2~4h of heating water bath refluxing extraction at 65 DEG C~85 DEG C, filtrate is concentrated after filtering and removes solvent, obtain tobacco concentrate, tobacco concentrate sodium hydroxide is adjusted into pH value 7.0, add neutral proteinase and in 40 DEG C~55 DEG C 3~4h of water-bath, the tobacco extract after neutral protease enzymolysis is made after concentration, tobacco extract is positioned in vacuum distillation equipment, concentrate is evaporated under reduced pressure, slow heating, system pressure is 1~5mmHg, less than 200 DEG C fraction As and 200 DEG C~300 DEG C of fraction B are collected respectively.The present invention can more thoroughly remove the protein in tobacco, so as to obtain, protein content is low, the preferable tobacco extract of perfuming effect.
Description
Technical field
The invention belongs to the preparation of technical field of tobacco, specifically a kind of low albumen tobacco extract for novel tobacco
Method and application.
Background technology
In recent years, with propulsion horizontal on cigarette, reduce the coke tar in cigarette amount of releasing and put the jealous deficiency of caused tobacco,
It is tobacco business urgent problem to be solved that satisfaction, which weakens,.
Tobacco extract is a kind of dark brown viscous lotion, available for tobacco nature fragrance is increased in tobacco essence, improves cigarette
Careless suction quality.At present, conventional method is tobacco material to be condensed into cream after polar solvent extracts or by Aqueous extracts through alcohol precipitation
Clear liquid is taken to be concentrated to give prepared opium paste after processing, the universal quality of prepared opium paste that similar technique is extracted is general, and there is contained in prepared opium paste
Aroma component relative amount is not high, and solvent consumption is larger and has the problems such as residual, can not meet that tobacco industry further develops
Demand.The highly desirable related researcher of tobacco business is capable of providing that can more to improve tobacco jealous for this, increases tobacco
The tobacco extract product of inherent feature fragrance, promotes the sustainable development of tobacco industry.
Protein tobacco is built up with the growth and development of tobacco, the exploitation profit as edible protein resource
With tobacco protein has its positive effect, and the influence for tobacco leaf sucking quality then needs to should be regarded as a different matter.By modulated flue-cured tobacco
Tobacco leaf protein content is generally 8% or so.In tobacco product, focus on tobacco aroma and inhale taste, and protein content mistake
Height, then fragrance and jealous variation, produce acid, bitter taste and irritation.
The content of the invention
In view of the deficiencies of the prior art, a kind of improvement tobacco of present invention offer is jealous, increase tobacco inherent feature fragrance
Tobacco extract, further isolates the tobacco extract of low-protein from tobacco extract.
Technical solution provided by the invention：A kind of preparation method of low albumen tobacco extract for novel tobacco, bag
Include following steps：
(1) using tobacco as raw material, pulverization process is carried out, according to feed liquid mass ratio 1:4~1:8 ratio adds mass fraction
60%~95% ethanol, heating water bath 2~4h of refluxing extraction at 65 DEG C~85 DEG C, filtrate is concentrated remove solvent after filtering,
Obtain tobacco concentrate；
(2) sodium hydroxide solution of the tobacco concentrate obtained by step (1) is adjusted into pH value 7.0, it is neutral adds 5.0g/L
The tobacco extract after neutral protease enzymolysis is simultaneously made in 40 DEG C~55 DEG C 3~4h of water-bath, after concentration for protease；
(3) tobacco extract obtained by step (2) is positioned in vacuum distillation equipment, decompression steaming is carried out to tobacco extract
Evaporate, be slowly heated, system pressure is 1~5mmHg, collects less than 200 DEG C fraction As and 200 DEG C~300 DEG C of fraction B respectively.
Further, the tobacco material is flue-cured tobacco, Turkish tobaccos, drying cigarette, burley tobaccos, cigar, Nicotiniana rustica.
Another technical solution provided by the invention：A kind of application of low albumen tobacco extract, will be according to above-mentioned preparation
It is 5 that fraction A made from method and fraction B, which take mass ratio,:2~5:7, after adding the propane diols dissolving of 6~8 times of distillate weight,
It is added in new cigarette tobacco product.
Further, the new cigarette tobacco product includes electrical heating new cigarette, carbon heating new cigarette, electronics
Cigarette, snuff and tobacco spray product.
Beneficial effects of the present invention：(1) protein in tobacco more can be thoroughly removed using enzymatic isolation method, reduces egg
Influence of the white matter to tobacco extract perfuming effect, so as to obtain, protein content is low, the preferable tobacco extract of perfuming effect；
(2) by being evaporated under reduced pressure the tobacco extract extracted, the influence of Nicotiana tabacum extract macromolecular substances is reduced；(3)
According to the characteristic of kinds of cigarettes product, tobacco extract will be extracted and be applied to novel tobacco product, make the characteristic perfume of tobacco
It is more readily apparent, improve the acceptable degree of novel tobacco product.
Embodiment
The technical solution in the present invention is clearly and completely described with reference to specific embodiment.It should be appreciated that this
The described specific embodiment in part can only be used to explain the present invention, be not intended to limit the present invention.
Embodiment one
Take 500g crush after burley tobaccos tobacco leaf, add 75% ethanol 2000mL and be heated to reflux 3h at 80 DEG C and carried
Take, filtrate is concentrated after filtering and removes solvent, burley tobaccos tobacco concentrate is obtained after concentration, by burley tobaccos extract hydroxide
After sodium adjusts pH value 7.0,5.0g/L neutral proteinases are added thereto and in 40 DEG C of water-bath 4h, obtained neutral proteinase after concentration
Bailei tobacco extract after enzymolysis.
Bailei tobacco extract is taken, is positioned in vacuum distillation equipment, concentrate is evaporated under reduced pressure, is slowly heated, system
Pressure is 1mmHg, collects less than 200 DEG C fraction As and 200 DEG C~300 DEG C of fraction B respectively.It is fraction A to take mass ratio：Fraction B
=5:4 distillate 1g, after being dissolved with 6g propane diols, is added in the electrical heating new cigarette tobacco product of thin slice preparation, can
It is more rich with stimulation of the reduction to throat, the reduction of miscellaneous gas, cigarette perfume.
Embodiment two
The cured tobacco leaf 500g after crushing is taken, 60% ethanol 4000mL is added and is heated to reflux 4h at 85 DEG C and is carried
Take, filtrate is concentrated after filtering and removes solvent, flue-cured tobacco tobacco concentrate is obtained after concentration, by flue-cured tobacco extract sodium hydroxide tune
After saving pH value 7.0,5.0g/L neutral proteinases are added thereto and in 55 DEG C of water-bath 4h, obtained neutral protease enzymolysis after concentration
Flue-cured tobacco medicinal extract afterwards.
Flue-cured tobacco medicinal extract is taken, is positioned in vacuum distillation equipment, concentrate is evaporated under reduced pressure, is slowly heated, system pressure
Power is 3mmHg, collects less than 200 DEG C fraction As and 200 DEG C~300 DEG C of fraction B respectively.It is fraction A to take mass ratio：Fraction B=
5:7 distillate 1g, after being dissolved with 7g propane diols, is added in the carbon heating new cigarette tobacco product of thin slice preparation.
Embodiment three
The burley tobaccos tobacco leaf 500g after crushing is taken, 95% ethanol 3000mL is added and is heated to reflux 3h at 65 DEG C and is carried
Take, filtrate is concentrated after filtering and removes solvent, burley tobaccos tobacco concentrate is obtained after concentration, by burley tobaccos extract hydroxide
After sodium adjusts pH value 7.0,5.0g/L neutral proteinases are added thereto and in 50 DEG C of water-bath 3h, obtained neutral proteinase after concentration
Bailei tobacco extract after enzymolysis.
Bailei tobacco extract is taken, is positioned in vacuum distillation equipment, concentrate is evaporated under reduced pressure, is slowly heated, system
Pressure is 5mmHg, collects less than 200 DEG C fraction As and 200 DEG C~300 DEG C of fraction B respectively.It is fraction A to take mass ratio：Fraction B
=5:2 distillate 1g, after being dissolved with 8g propane diols, is added in electronic cigarette.
After low albumen tobacco extract adds cigarette products made from above-described embodiment 1~3, cigarette test sample is made.
Comparative sample：Conventional cigarette is prepared into pipe tobacco of the same race.Smoked panel test, commented respectively to test sample and comparative sample by 7 professionals
Suction the results are shown in Table shown in 1：
Table 1
Summarized through smokeing panel test, low albumen tobacco extract is prepared using the method for the present invention is exerted into pipe tobacco cigarette is made,
Compared with not adding the cigarette of the low albumen tobacco extract of the present invention, the addition present invention is low, and cigarette is made in albumen tobacco extract
Aroma quality and perfume quantity are greatly improved, and oral cavity throat excitement does not increase, and effectively lift the quality of cigarette.
The foregoing is merely the detailed description of specific embodiments of the present invention, does not limit the present invention with this, all at this
All any modification, equivalent and improvement made in the mentality of designing of invention etc., should be included in protection scope of the present invention it
It is interior.
Claims (1)
1. the preparation method of a kind of low albumen tobacco extract for novel tobacco, it is characterised in that include the following steps：
(1) the cured tobacco leaf 500g after crushing is taken, 60% ethanol 4000mL is added and is heated to reflux 4h at 85 DEG C and is extracted,
Filtrate is concentrated after filtering and removes solvent, flue-cured tobacco tobacco concentrate is obtained after concentration；
(2) by flue-cured tobacco tobacco concentrate with sodium hydroxide adjust pH value 7.0 after, thereto add 5.0g/L neutral proteinases and
55 DEG C of water-bath 4h, are made the flue-cured tobacco medicinal extract after neutral protease enzymolysis after concentration；
(3) flue-cured tobacco medicinal extract is taken, is positioned in vacuum distillation equipment, concentrate is evaporated under reduced pressure, is slowly heated, system pressure
For 3mmHg, less than 200 DEG C fraction As and 200 DEG C~300 DEG C of fraction B are collected respectively；
It is fraction A to take mass ratio：Fraction B=5:7 distillate 1g, after being dissolved with 7g propane diols, is added to the carbon of thin slice preparation
Heat in new cigarette tobacco product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610111815.6A CN105747271B (en) | 2016-02-29 | 2016-02-29 | A kind of preparation method of low albumen tobacco extract for novel tobacco |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610111815.6A CN105747271B (en) | 2016-02-29 | 2016-02-29 | A kind of preparation method of low albumen tobacco extract for novel tobacco |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105747271A CN105747271A (en) | 2016-07-13 |
| CN105747271B true CN105747271B (en) | 2018-05-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610111815.6A Active CN105747271B (en) | 2016-02-29 | 2016-02-29 | A kind of preparation method of low albumen tobacco extract for novel tobacco |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108077997A (en) * | 2017-12-12 | 2018-05-29 | 江西中烟工业有限责任公司 | A kind of preparation method and applications of cigarette filter combination |
| CN108451003B (en) * | 2018-01-30 | 2020-08-07 | 湖北中烟工业有限责任公司 | Method for preparing burley tobacco reactant by using hydrolase and application |
| CN109211720A (en) * | 2018-11-17 | 2019-01-15 | 中国烟草总公司郑州烟草研究院 | A method of preparing the one-stop measurement tobacco dichloromethane extract content of metering |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102604740A (en) * | 2012-03-08 | 2012-07-25 | 河南中烟工业有限责任公司 | Tobacco extract, and extraction method and application thereof |
| CN103564645A (en) * | 2012-07-18 | 2014-02-12 | 朱大恒 | Method and application for producing tobacco extracts through field tobacco waste |
| CN103919275B (en) * | 2014-04-17 | 2016-01-20 | 上海烟草集团有限责任公司 | The method that offal prepares tobacco aromatics is discarded in a kind of complex enzyme catalysis |
| CN104705779B (en) * | 2015-01-28 | 2016-08-17 | 中国烟草总公司郑州烟草研究院 | A kind of processing method improving cut stem quality |
| CN105249519B (en) * | 2015-11-13 | 2017-03-29 | 中国烟草总公司郑州烟草研究院 | A kind of reconstituted tobacco coating liquid and preparation method thereof suitable for new cigarette |
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| CN105747271A (en) | 2016-07-13 |
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