CN102604740A - Tobacco extract, and extraction method and application thereof - Google Patents

Abstract

The invention belongs to the technical field of tobacco spices, in particular to a tobacco extract and its extraction method and application. The extract is obtained by extracting tobacco stalk bast. The invention turns tobacco agricultural wastes into treasures, fully utilizes the resources, fully considers the feasibility and operability of industrialized production in the design of the preparation technical route, and extracts pure natural tobacco Homogenous spices have great market potential. The invention is convenient to be applied and realized in the existing cigarette processing technology, and can obviously improve the smoking quality of the cigarette.

Description

A kind of tobacco extract and its extraction method and application

technical field

The invention belongs to the technical field of tobacco spices, in particular to a tobacco extract and its extraction method and application.

Background technique

my country is a big tobacco-producing country. In tobacco agricultural production, a large number of tobacco stems are discarded every year. After the tobacco leaves are completely harvested, farmers directly discard the tobacco stems in the field or the field, which not only causes field traffic, irrigation blockage, and the breeding and transmission of diseases and insect pests. And its leaching solution will pollute the soil, and if it is dried and incinerated, it will pollute the atmosphere, causing a great waste of resources. If these tobacco stalks can be utilized, not only can the pollution caused by them be avoided, but also more value can be created by turning waste into treasure.

Contents of the invention

The purpose of the present invention is to provide a tobacco extract and its extraction method and application, which uses agricultural waste tobacco stems as raw materials, and the process of turning waste into treasure also avoids environmental pollution.

The technical scheme that the present invention adopts is as follows:

A tobacco extract, which is obtained by extracting tobacco stalk bast.

The extraction process is as follows: the tobacco stalk bast powder is extracted with ethanol, the extract is adjusted to be neutral, and then enzymatically hydrolyzed, then concentrated and purified under reduced pressure to obtain the extract.

Add 40-80 mesh tobacco stalk bast powder into 80-95% ethanol with a volume fraction of 4-6 times its mass, reflux extraction at 60-80°C for 2-4 hours, and then cool and filter.

Adjust the pH of the extract to 6.0-7.5.

Add neutral protease with a mass concentration of 6.0 g/L to the pH-adjusted extract, extract at 35-55°C for 3-6 hours, and concentrate under reduced pressure at 40-80°C to obtain a crude extract; the neutral protease The enzyme activity is 50,000 u/g.

The crude extract of the extract is 3-4 times the mass of ethanol with a volume fraction of 95% as the extractant, and the extract is obtained after distillation.

Specifically, the extraction process is as follows: Add 40-80 mesh tobacco stalk bast powder into ethanol with a volume fraction of 95% of its mass, reflux at 80°C for 3 hours, cool and filter, adjust the pH value to 7.0, and add Neutral protease with a mass concentration of 6.0g/L, extracted at 40°C for 4h, concentrated under reduced pressure at 80°C to form a crude extract of extract; The extractant is obtained after distillation.

The extract is used for flavoring the tobacco, and the dosage is 0.05-0.10% of the weight of the tobacco leaves.

The extract is diluted with water or ethanol and then sprayed into the tobacco leaves.

The invention utilizes the bast of the tobacco stalk, extracts a large amount of natural tobacco fragrance components contained therein, and then applies it to the tobacco product. After analysis and determination, the tobacco extract provided by the present invention mainly contains more than 20 kinds of flavor substances such as nicotine, aldehydes and ketones, alcohols, terpenes, and nitrogen heterocycles. Cigarette aroma and aroma, increase the fullness of smoke and smoking satisfaction, alcohol and smoke, cover up miscellaneous gas, and improve aftertaste. The extraction method provided by the present invention mainly starts from the perspective of environment and resources, and turns tobacco agricultural waste into treasure, so that resources are fully utilized. At the same time, the method also has the advantages of simple operation and high extraction rate. The extracted extract is a pure natural tobacco homologous spice, which has strong operability and market potential. The provided application is also very convenient, can be easily realized in the existing cigarette processing technology, and can obviously improve the smoking quality of the cigarette.

Compared with the prior art, the present invention has the following advantages:

The invention turns tobacco agricultural wastes into treasures, fully utilizes the resources, fully considers the feasibility and operability of industrialized production in the design of the preparation technical route, and extracts pure natural tobacco Homogenous spices have great market potential. The invention is convenient to be applied and realized in the existing cigarette processing technology, and can obviously improve the smoking quality of the cigarette.

Detailed ways

The technical scheme of the present invention is described below with specific examples, but protection scope of the present invention is not limited to this:

Example 1

Grind the bast of the naturally dried tobacco stems into 80 meshes, add 95% (volume fraction, the same below) ethanol which is 6 times its mass, extract by reflux at 80°C for 3 hours, cool and filter, and adjust the pH with sodium hydroxide value to 7.0, add neutral protease with a mass concentration of 6.0g/L (enzyme activity 50,000 u/g), extract at 40°C for 4h, and concentrate at 80°C to obtain a crude extract. For the crude extract of the extract, use 95% ethanol which is 3 times its mass as the extractant, and the extract is obtained after distillation. The obtained extract is placed in a brown bottle and sealed and refrigerated.

Dilute the obtained extract with water to 1% (wt%, the same below), according to the ratio of adding 50g (the addition ratio of the extract is 0.05%) of the diluent to 1000g of tobacco leaves, spray it evenly into the leaves, place it at room temperature for 2h, absorb Finally, cut into shreds, dry shreds, and make cigarettes according to the normal process.

Example 2

Grind the naturally dried tobacco stem bast into 60 meshes, add 5 times its mass of 90% ethanol, reflux extraction at 70°C for 4 hours, cool and filter, adjust the pH value to 6.5 with sodium hydroxide, and add a mass concentration of 6.0g/L of neutral protease (50,000 u/g), extracted at 50°C for 5h, concentrated at 70°C to obtain a crude extract. For the crude extract of the extract, 4 times the volume fraction of 95% ethanol is used as the extractant, and the extract is obtained after distillation. The obtained extract is placed in a brown bottle and sealed and refrigerated.

Dilute the obtained extract with water to 2%, add 50g (the addition ratio of the extract is 0.10%) of diluent to 1000g of tobacco leaves, evenly spray it into the leaves, place it at room temperature for 2h, and cut it into shreds according to the normal process after absorption. Dried silk, cigarettes.

Example 3

Grind the naturally dried tobacco stem bast into 70 meshes, add 6 times of ethanol with a volume fraction of 85%, reflux extraction at 80°C for 4 hours, cool and filter, adjust the pH value to 7.0 with sodium hydroxide, and add a mass concentration of 6.0 g/L neutral protease (50,000 u/g), extract at 55°C for 6h, and concentrate at 60°C to obtain crude extract. For the crude extract of the extract, 3 times the volume fraction of 95% ethanol is used as the extractant, and the extract is obtained after distillation. The obtained extract is placed in a brown bottle and sealed and refrigerated.

Dilute the obtained extract to 3% with ethanol with a volume fraction of 75%, and spray it evenly into the leaves according to the ratio of adding 50g (the addition ratio of the extract is 0.15%) to 1000g of tobacco leaves, and place it at room temperature for 2 hours. Shredded, dried and rolled according to the normal process.

Example 4

Grind the naturally dried tobacco stem bast into 40 meshes, add 4 times the volume fraction of 95% ethanol, reflux extraction at 60°C for 4 hours, cool and filter, adjust the pH value to 6.0 with sodium hydroxide, and add a mass concentration of 6.0 g/L neutral protease (50,000 u/g), extract at 35°C for 6h, and concentrate at 50°C to obtain crude extract. For the crude extract of the extract, 3 times the volume fraction of 95% ethanol is used as the extractant, and the extract is obtained after distillation. The obtained extract is placed in a brown bottle and sealed and refrigerated.

Smoking test

The extracts of Examples 1, 2, and 3 were added to the tobacco leaves according to the corresponding methods and proportions to make three groups of samples, and the tobacco leaves without the extract were used as the control sample, and the sensory evaluation was carried out respectively. The evaluation results are shown in the table 1.

Table 1 Sensory Quality Inspection Form

From the test results in Table 1, it can be seen that the tobacco extract of the present invention can significantly improve the aroma and aroma of cigarettes, increase the fullness of smoke and the satisfaction of smoking, alcohol and smoke, cover up miscellaneous gases, and improve aftertaste.

The tobacco extract obtained in embodiment 1 is carried out GC/MS analysis, and the results are as shown in table 1:

Table 1

serial number Retention time (min) chemical composition Relative content (%) 1 49.612 DMF 0.493 2 59.437 Acetic acid 1.052 3 60.592 Furfural 0.932 4 64.894 formic acid 0.160 5 67.998 5-Methylfurfural 0.453 6 68.687 1,2-propanediol 0.173 7 73.145 furan methanol 0.306 8 73.422 Methyl palmitate 0.120 9 76.814 β-phellandrene 0.360 10 84.543 Nicotine 56.426 11 85.293 Benzyl alcohol 0.093 12 90.127 Maltol 0.067 13 91.225 2,5-Furancarboxylic acid 0.266 14 91.948 phenol 0.107 15 93.405 pantolactone 0.093 16 94.035 pyrrolidone 0.120 17 98.789 4-octen-3-one 0.200 18 100.324 Valeraldehyde 0.053 19 101.139 Methyl palmitate 0.320 20 101.309 Mesmin 0.093 twenty one 102.292 2,2'furfural 0.493 twenty two 104.374 4(H)-pyran-4-one 2.064 twenty three 105.145 6-Ethylquinoline 0.306 twenty four 105.712 macrostigmatrienone 0.240 25 106.912 Ribitol 0.173 26 107.355 4H-3,5-furandiol 0.107 27 114.508 5-Hydroxymethylfurfural 15.235 28 132.703 Palmitic acid 19.257

The above-mentioned embodiment is the preferred embodiment of the present invention, but the embodiment of the present invention is not limited by the above-mentioned embodiment, and any other changes that do not deviate from the present invention should be equivalent replacement methods, and are all included in the present invention. within the scope of protection.

Claims (10)

1. A tobacco extract, characterized in that the extract is extracted from tobacco stalk bast. 2. The tobacco extract as claimed in claim 1, wherein the extraction process is as follows: extract the tobacco stalk bast powder with ethanol, adjust the extract to neutrality, perform enzymatic hydrolysis, and then concentrate and purify under reduced pressure Obtain the extract. 3. tobacco extract as claimed in claim 2, is characterized in that, extraction process is: the tobacco stalk bast powder of 40-80 purpose is added the ethanol that its volume fraction of 4-6 times is 80-95% reflux extraction at 60-80°C for 2-4h, then cool and filter, adjust the pH value to 6.0-7.5, add neutral protease with a mass concentration of 6.0g/L, extract at 35-55°C for 3-6h, reduce at 40-80°C Pressing and concentrating into the crude extract of the extract; the volume fraction of 3-4 times the volume fraction of the crude extract of the extract is 95% ethanol as the extractant, and the extract is obtained after distillation, and the neutral protease The enzyme activity is 50,000 u/g. 4. A method for extracting tobacco extract, which is characterized in that the bast powder of tobacco stems is extracted with ethanol, the extract is adjusted to neutrality, followed by enzymatic hydrolysis, and then concentrated and purified under reduced pressure to obtain the extract. 5. the extraction method of tobacco extract as claimed in claim 4 is characterized in that, the tobacco stalk bast powder of 40-80 purpose is added in the ethanol that its volume fraction of 4-6 times is 80-95% , Reflux extraction at 60-80°C for 2-4h, then cool and filter. 6. The extraction method of tobacco extract as claimed in claim 5, characterized in that the pH of the extract is adjusted to 6.0-7.5. 7. the extraction method of tobacco extract as claimed in claim 6 is characterized in that, the neutral protease that mass concentration is 6.0g/L is added in the extracting solution that has adjusted pH value, 35-55 ℃ extracts 3-6h, Concentrate under reduced pressure at 40-80°C to obtain the crude extract of the extract; the enzyme activity of the neutral protease is 50,000 u/g. 8. the extracting method of tobacco extract as claimed in claim 7 is characterized in that, the ethanol of 95% volume fraction is made extraction agent with the crude extract crude extract quality its 3-4 times, obtains after distillation The extract. 9. The application of the tobacco extract according to claim 1, characterized in that, the extract is used for tobacco flavoring in an amount of 0.05-0.10% of the tobacco leaf weight. 10. The application of the tobacco extract as claimed in claim 9, characterized in that the extract is diluted with water or ethanol and then sprayed into the tobacco leaves.