CN105745336B - 用于从混合物富集突变核酸的方法 - Google Patents

用于从混合物富集突变核酸的方法 Download PDF

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Publication number
CN105745336B
CN105745336B CN201480064597.4A CN201480064597A CN105745336B CN 105745336 B CN105745336 B CN 105745336B CN 201480064597 A CN201480064597 A CN 201480064597A CN 105745336 B CN105745336 B CN 105745336B
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nucleic acid
oligonucleotides
allele
mispairing
target nucleic
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CN105745336A (zh
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N.舍恩布伦纳
A.古普塔
K.延森
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201480064597.4A 2013-11-27 2014-11-25 用于从混合物富集突变核酸的方法 Active CN105745336B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361909587P 2013-11-27 2013-11-27
US61/909587 2013-11-27
PCT/EP2014/075460 WO2015078831A1 (en) 2013-11-27 2014-11-25 Methods for the enrichment of mutated nucleic acid from a mixture

Publications (2)

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CN105745336A CN105745336A (zh) 2016-07-06
CN105745336B true CN105745336B (zh) 2019-03-01

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US (1) US9873908B2 (https=)
EP (1) EP3074529B1 (https=)
JP (1) JP6553598B2 (https=)
CN (1) CN105745336B (https=)
CA (1) CA2926693C (https=)
ES (1) ES2676062T3 (https=)
WO (1) WO2015078831A1 (https=)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2011224534B2 (en) 2010-03-08 2014-10-09 Dana-Farber Cancer Institute, Inc. Full COLD-PCR enrichment with reference blocking sequence
EP2691541B1 (en) 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences
ES2986436T3 (es) 2011-04-15 2024-11-11 Univ Johns Hopkins Sistema de secuenciación segura
ES2886507T5 (es) 2012-10-29 2024-11-15 Univ Johns Hopkins Prueba de Papanicolaou para cánceres de ovario y de endometrio
CA2990846C (en) 2015-06-24 2023-09-26 Dana-Farber Cancer Institute, Inc. Selective degradation of wild-type dna and enrichment of mutant alleles using nuclease
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
WO2018111835A1 (en) 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
CN111868260B (zh) 2017-08-07 2025-02-21 约翰斯霍普金斯大学 用于评估和治疗癌症的方法和材料
CN112400026A (zh) * 2018-02-26 2021-02-23 Cy分子诊断公司 用于稀有种类亲和定向富集的组合物和方法
JP6947464B2 (ja) 2018-07-19 2021-10-13 バイオフィデリティ・リミテッド 改善されたポリヌクレオチド配列検出法
GB201919186D0 (en) 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
EP4103748A4 (en) 2020-02-14 2024-03-13 The Johns Hopkins University METHOD AND MATERIALS FOR ASSESSING NUCLEIC ACIDS
US11634754B2 (en) 2021-04-15 2023-04-25 Biofidelity Ltd. Nucleic acid enrichment and detection
CN113774113B (zh) * 2021-09-17 2023-07-04 广州医科大学附属第一医院（广州呼吸中心） 一种富集突变基因序列的方法和应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6120992A (en) * 1993-11-04 2000-09-19 Valigene Corporation Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, and allele identification in a diseased human
WO2002086196A1 (en) * 2001-04-19 2002-10-31 Rd Chemical Company Copper acid baths, system and method for electroplating high aspect ratio substrates
WO2007084380A2 (en) * 2006-01-13 2007-07-26 California Institute Of Technology Compounds and methods for nucleic acid mismatch detection
CN103215361A (zh) * 2013-04-18 2013-07-24 深圳联合医学科技有限公司 等位基因变体检测方法、试剂盒及组合物

Family Cites Families (14)

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US6630301B1 (en) 1997-03-14 2003-10-07 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum
AU2324997A (en) 1996-03-15 1997-10-01 Penn State Research Foundation, The Detection of extracellular tumor-associated nucleic acid in blood plasma or ser um using nucleic acid amplification assays
US6031098A (en) 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
SE9902741D0 (sv) 1999-07-19 1999-07-19 Boerries Kemper Method for the enrichment of heteroduplexes and its use in mutation detection
US20030082537A1 (en) * 2000-05-23 2003-05-01 Stanton Vincent P. Methods for genetic analysis of DNA to detect sequence variances
US20020132244A1 (en) 2000-09-27 2002-09-19 Xiao-Cheng Li-Sucholeiki Methods for detecting rare polymorphic variants in genomic DNA sequences
US6808884B2 (en) * 2000-12-08 2004-10-26 California Institute Of Technology Methods and compositions for detecting polynucleotide duplex damage and errors
WO2002086169A1 (en) 2001-04-23 2002-10-31 Dana-Farber Cancer Institute, Inc. Methods for rapid screening of polymorphisms, mutations and methylation
GB2382137A (en) 2001-11-20 2003-05-21 Mats Gullberg Nucleic acid enrichment
WO2005085473A2 (en) * 2004-03-01 2005-09-15 University Of Chicago Polymorphisms in the epidermal growth factor receptor gene promoter
WO2007047572A2 (en) 2005-10-14 2007-04-26 Cleveland State University Methods for identifying multiple dna alteration markers in a large background of wild-type dna
US20070154892A1 (en) 2005-12-30 2007-07-05 Simon Wain-Hobson Differential amplification of mutant nucleic acids by PCR in a mixure of nucleic acids
AU2011224534B2 (en) 2010-03-08 2014-10-09 Dana-Farber Cancer Institute, Inc. Full COLD-PCR enrichment with reference blocking sequence
US20120164641A1 (en) 2010-12-22 2012-06-28 Roche Molecular Systems, Inc. Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6120992A (en) * 1993-11-04 2000-09-19 Valigene Corporation Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, and allele identification in a diseased human
WO2002086196A1 (en) * 2001-04-19 2002-10-31 Rd Chemical Company Copper acid baths, system and method for electroplating high aspect ratio substrates
WO2007084380A2 (en) * 2006-01-13 2007-07-26 California Institute Of Technology Compounds and methods for nucleic acid mismatch detection
CN103215361A (zh) * 2013-04-18 2013-07-24 深圳联合医学科技有限公司 等位基因变体检测方法、试剂盒及组合物

Also Published As

Publication number Publication date
JP6553598B2 (ja) 2019-07-31
EP3074529A1 (en) 2016-10-05
CA2926693C (en) 2018-06-19
CN105745336A (zh) 2016-07-06
ES2676062T3 (es) 2018-07-16
US9873908B2 (en) 2018-01-23
JP2016539624A (ja) 2016-12-22
EP3074529B1 (en) 2018-05-02
WO2015078831A1 (en) 2015-06-04
US20150147760A1 (en) 2015-05-28
CA2926693A1 (en) 2015-06-04

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