CN105724134A - Striped flea beetle biological control method adopting leaf mustard as trapping crop - Google Patents

Striped flea beetle biological control method adopting leaf mustard as trapping crop Download PDF

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Publication number
CN105724134A
CN105724134A CN201610107888.8A CN201610107888A CN105724134A CN 105724134 A CN105724134 A CN 105724134A CN 201610107888 A CN201610107888 A CN 201610107888A CN 105724134 A CN105724134 A CN 105724134A
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China
Prior art keywords
caulis
folium brassicae
brassicae junceae
control method
biological control
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CN201610107888.8A
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Chinese (zh)
Inventor
陈学新
任少鹏
谭辉
杨帆
闫成进
邹佳妮
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Zhejiang University ZJU
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Zhejiang University ZJU
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Priority to CN201610107888.8A priority Critical patent/CN105724134A/en
Publication of CN105724134A publication Critical patent/CN105724134A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Botany (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to biological control over plant pests, and aims at providing a striped flea beetle biological control method adopting leaf mustard as a trapping crop. The striped flea beetle biological control method adopting the leaf mustard as the trapping crop comprises the specific steps that the leaf mustard is selected as a trapping plant, vegetables and the leaf mustard are subjected to seedling growing in a greenhouse at the same time, and transplanted to a field piece together after 30 days, and the vegetables and the leaf mustard are managed according to normal fertilizer and water of the vegetables; when more than two thirds of the areas of leaves of the leaf mustard are damaged, the leaf mustard is sleeved with bags and moved away for centralized treatment, and meanwhile new leaf mustard is supplemented to the field piece. The leaf mustard is utilized for attracting the pests and then subjected to centralized treatment, the number of adult pests is effectively reduced, larvae, pupas and eggs hard to control in soil are considered, a new way is provided for efficient control over striped flea beetles, and the killing effect on the striped flea beetles is good.

Description

A kind of phyllotreta striolata biological control method using Caulis et Folium Brassicae junceae as bait crop
Technical field
The present invention is about plant insect field of biological control, particularly to a kind of phyllotreta striolata biological control method using Caulis et Folium Brassicae junceae as bait crop.
Background technology
Phyllotreta striolata Phyllotratastriolata (Fabricius) belongs to coleoptera, Chrysomelidae insect, it is commonly called as Canis familiaris L. mite, flea, it is called for short flea beetle, often cause harm leaf vegetables, based on brassicaceous vegetables such as Caulis et Folium Brassicae capitatae, Brassica oleracea L. var. botrytis L., Chinese cabbage, tender flower stalk, Radix Raphani, Radix Brassicae rapae, Brassica campestris Ls, but solanaceous vegetables of also causing harm, melon, legume vegetable.
For a long time, the control for phyllotreta striolata relies primarily on Agro-chemicals control, and many to prevent and treat adult.Foliage application cannot take into account larva in soil, pupa and ovum, medicament lasting period one mistake, and adult constantly sprouts wings to be unearthed and causes harm, but increase spray medicine number of times and strengthen dosage, not only cause that insecticide preventive effect declines so that it is develop immunity to drugs, and serious environment pollution, reduces quality of vegetable.
Although taking corresponding measure in the last few years, such as:
(1) scientist such as selection-breeding and utilization: Brett of pest-resistant cultivar filters out 19 kinds that phyllotreta striolata has resistance from the vegetables such as common head cabbage, Brassica Oleracea Var.Acephala, brocoli, Brussels soup dish.Stinner finds that phyllotreta striolata is had notable resistance by leaf surface lanate broccoli kind.The scientists such as Bonfils pass through Protoplast cuhnre and the somatocyte hybriding technology of leaf callus, are imported in Cruciferae Vegetables in Brassica by wild plant anti insect gene and succeed, explore new way for phyllotreta striolata breeding for pest resistance.
(2) utilization of plant secondary compounds: over nearly 20 years, compare active about plant secondary compounds with the research of phyllotreta striolata mutual relation, the research preventing and treating phyllotreta striolata with plant secondary substance is relatively broad.Result of study shows, phyllotreta striolata adult is produced very strong Antifeedant Effects by the commercial soap of foliage-spray 0.05%.Hou Youming systematically have studied nimbin to the biological activity of phyllotreta striolata and the mechanism of action, result shows, nimbin is obvious to the control effectiveness comparison of phyllotreta striolata population, spray at heart of a cabbage cotyledon period with 200~1800 times of liquid of 1% nimbin emulsion, the seedling protecting effect of 15d reaches 81.9%~82.4%, and attacked trees rate substantially reduces.
(3) R and D of biological pesticide: in recent years, China's application entomopathogenic nematode preventing and treating phyllotreta striolata has had more deep research.Entomopathogenic nematode has good disinsection effect, the feature such as higher plant is safe, free from environmental pollution and easy to use, is the new bio insecticide received much attention in the world at present.Especially soil border of dwelling is hidden, that be difficult to prevent and treat with chemical pesticide class pest of dwelling has obvious prevention effect.Entomopathogenic nematode for preventing and treating phyllotreta striolata larva mainly has genus steinernema Stienernema and mass production Heterorhabditis.
But these technical method R&D costs are higher, take time and effort, and still there is the risk making insect produce resistance.
Summary of the invention
Present invention is primarily targeted at and overcome deficiency of the prior art, it is provided that a kind of can efficiently prevent and treat the new method that phyllotreta striolata puies forward.For solving above-mentioned technical problem, the solution of the present invention is:
A kind of phyllotreta striolata biological control method using Caulis et Folium Brassicae junceae as bait crop is provided, during for growing vegetables, carries out the preventing and treating of phyllotreta striolata, described phyllotreta striolata biological control method using Caulis et Folium Brassicae junceae as bait crop particularly as follows:
Choose Caulis et Folium Brassicae junceae as Trap plants, vegetable and Caulis et Folium Brassicae junceae are carried out nursery simultaneously in greenhouse, transplants together after 30 days;Vegetable is directly transplanted to field, and Caulis et Folium Brassicae junceae is transplanted to flowerpot, then has the flowerpot of Caulis et Folium Brassicae junceae to put in kind of the field being implanted with vegetable by planting, and places a line Trap plants (namely at interval of three row vegetables, arrange a line Caulis et Folium Brassicae junceae) in field in every four row plants;
To the vegetable in field and Caulis et Folium Brassicae junceae all according to the normal rich water quality management of vegetable;
(start nursery about 65 days from vegetable and Caulis et Folium Brassicae junceae) when mustard leaf area is compromised more than 2/3rds, Caulis et Folium Brassicae junceae bagging is removed and focuses on, mend in field simultaneously and put new Caulis et Folium Brassicae junceae.
In the present invention, described Trap plants adopts Wenzhou Caulis et Folium Brassicae junceae, i.e. slow mustard.
In the present invention, focusing on after described Caulis et Folium Brassicae junceae bagging, particularly as follows: by (including soil to be inserted in together in bag, remove flowerpot) after Caulis et Folium Brassicae junceae bagging, put into the frozen coating of refrigerator-freezer or refrigerator, take out after 2 hours and abandon.
In the present invention, the row in described field is finger widths is the field of 1m, and field along wide two vegetables of plantation side by side or places two flowerpots.
In the present invention, described flowerpot adopts basin mouth diameter to be diameter at the bottom of 25cm, basin is 15cm, basin height to be the flowerpot of 20cm.
In the present invention, in the flowerpot of described plantation Caulis et Folium Brassicae junceae, each flowerpot is only planted a Caulis et Folium Brassicae junceae.
In the present invention, described vegetable is Caulis et Folium Brassicae capitatae.
Compared with prior art, the invention has the beneficial effects as follows:
The present invention utilizes Caulis et Folium Brassicae junceae to attract insect, then concentrate and processed, not only effectively reduce the quantity of adult, also taken into account the larva, pupa and the ovum that are difficult to prevent and treat in soil, a kind of new approach and the good disinsection effect to phyllotreta striolata is provided for efficiently preventing and treating phyllotreta striolata.
Comparing traditional prevention and controls, the present invention can reduce a large amount of recruitment expenses of the cultural control such as cost of use and artificial deinsectization of chemical pesticide, and simple to operate, cost is low, easily promotes.
The present invention does not use chemical pesticide, is absent from pesticide residues, to people, animal safety, simultaneously without contaminated environment, maintains ecological balance, has good economy, society and ecological benefits.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is described in further detail:
The following examples can make the professional and technical personnel of this specialty more fully understand the present invention, but does not limit the present invention in any way.Concrete operations are as follows:
(1) experimental field overview
Being Wenzhou City, Zhejiang Province Wenzhou Vocational & Technical College rattan abutment ground for examination cabbage field, area is about 13340m2
(2) experimental field plant layout
Field comprises Caulis et Folium Brassicae capitatae and Caulis et Folium Brassicae junceae two kind of plant.Field is divided into some ridges of south-north direction, is 1m width.Caulis et Folium Brassicae capitatae takes bunch planting way south-north direction to plant, and Caulis et Folium Brassicae junceae is taked potted plant.With one little entirety of 4 behavior, front 3 behavior Caulis et Folium Brassicae capitataes, the 4th row plantation Caulis et Folium Brassicae junceae, the plantation of whole field is by that analogy.
Field is according to the normal rich water quality management in Caulis et Folium Brassicae capitatae ground.
(3) implantation time of plant
Experimental period chooses the 4-6 month of insect great outburst, Caulis et Folium Brassicae capitatae, and Caulis et Folium Brassicae junceae is transplanted after temperature indoor raising of seedling.
(4) test survey method
The start time of field investigation is 50 days after Caulis et Folium Brassicae capitatae and Caulis et Folium Brassicae junceae nursery, within every 7 days, investigates once, totally 5 times.Meeting the impact of non-artificial objective factor or other situations being not suitable for investigation then suitably adjust control time, concrete investigation method is as follows:
This experiment adopts diagonal 5 point sampling, and for gathering all phyllotreta striolatas in sampling point as far as possible accurately, each sampling point adopts net to sweep, yellow plate is pasted, trapping method and direct visual perception method are investigated.
Randomly selecting the square of five 1m × 1m, above plant, 10cm place hangs yellow plate, places 24 hours.It is collected in the phyllotreta striolata on indoor qualification Huang plate statistical magnitude;
Sweep the phyllotreta striolata of flight in sample prescription with net simultaneously, every piece of sample prescription is for 20 times a net back and forth, is fitted in easy envelope to take back test chamber by interior all the things of beating, steeps in 95% ethanol after chilled process, remove other insecticides under the microscope, to phyllotreta striolata counting, taxonomic identification;
While placing yellow plate, sample prescription digs the pitting of about the 10cm degree of depth, it is placed with and lures collection solution (sugar: vinegar: ethanol: water=2:1:1:20, add a small amount of liquid detergent again) sanitary cup, ensure that cup is along concordant with ground, place 24 hours, collect phyllotreta striolata the statistical magnitude creeped in earth's surface.
Five sampling points in Caulis et Folium Brassicae capitatae and Caulis et Folium Brassicae junceae, each sampling point takes 4 strain plant.With direct visual perception method, the quantity of itemized record plant leaf all visible phyllotreta striolatas on the front and back.
(5) survey result
The contrast on Caulis et Folium Brassicae capitatae and Caulis et Folium Brassicae junceae of table 1 phyllotreta striolata
Note: with in the Different Crop of a line, the difference that different lower cases illustrates between crop reaches significant level (onewayANOVA;P < 0.05)
As can be seen from Table 1, the phyllotreta striolata quantity on Caulis et Folium Brassicae junceae to be significantly higher than Caulis et Folium Brassicae capitatae, it was demonstrated that phyllotreta striolata is had significant sucking action by Caulis et Folium Brassicae junceae.During second time (May 14) and third time (May 21) investigation, quantity peaks especially, therefore, after Caulis et Folium Brassicae capitatae and Caulis et Folium Brassicae junceae start nursery about 65 days, when mustard leaf area is compromised more than 2/3rds, will (include soil to be inserted in together in bag, remove flowerpot) after Caulis et Folium Brassicae junceae bagging, put into the frozen coating of refrigerator-freezer or refrigerator, take out after 2 hours and abandon, mend in time simultaneously and put new Caulis et Folium Brassicae junceae, so can reach the prevention effect of the best.
Finally it should be noted that listed above is only specific embodiments of the invention.It is clear that the invention is not restricted to above example, it is also possible to there is many variations.All deformation that those of ordinary skill in the art can directly derive from present disclosure or associate, are all considered as protection scope of the present invention.

Claims (7)

1., using Caulis et Folium Brassicae junceae as a phyllotreta striolata biological control method for bait crop, during for growing vegetables, carry out the preventing and treating of phyllotreta striolata, it is characterised in that described phyllotreta striolata biological control method using Caulis et Folium Brassicae junceae as bait crop particularly as follows:
Choose Caulis et Folium Brassicae junceae as Trap plants, vegetable and Caulis et Folium Brassicae junceae are carried out nursery simultaneously in greenhouse, transplants together after 30 days;Vegetable is directly transplanted to field, and Caulis et Folium Brassicae junceae is transplanted to flowerpot, then has the flowerpot of Caulis et Folium Brassicae junceae to put in kind of the field being implanted with vegetable by planting, and places a line Trap plants in field in every four row plants;
To the vegetable in field and Caulis et Folium Brassicae junceae all according to the normal rich water quality management of vegetable;
When mustard leaf area is compromised more than 2/3rds, Caulis et Folium Brassicae junceae bagging is removed and focuses on, mend in field simultaneously and put new Caulis et Folium Brassicae junceae.
2. a kind of phyllotreta striolata biological control method using Caulis et Folium Brassicae junceae as bait crop according to claim 1, it is characterised in that described Trap plants adopts Wenzhou Caulis et Folium Brassicae junceae, i.e. slow mustard.
3. a kind of phyllotreta striolata biological control method using Caulis et Folium Brassicae junceae as bait crop according to claim 1, it is characterized in that, focusing on after described Caulis et Folium Brassicae junceae bagging, particularly as follows: by after Caulis et Folium Brassicae junceae bagging, put into the frozen coating of refrigerator-freezer or refrigerator, take out after 2 hours and abandon.
4. a kind of phyllotreta striolata biological control method using Caulis et Folium Brassicae junceae as bait crop according to claim 1, it is characterised in that the row in described field is finger widths is the field of 1m, and field along wide side by side plantation two vegetables or place two flowerpots.
5. a kind of phyllotreta striolata biological control method using Caulis et Folium Brassicae junceae as bait crop according to claim 4, it is characterised in that described flowerpot adopts basin mouth diameter to be diameter at the bottom of 25cm, basin be 15cm, basin height to be the flowerpot of 20cm.
6. a kind of phyllotreta striolata biological control method using Caulis et Folium Brassicae junceae as bait crop according to claim 5, it is characterised in that in the flowerpot of described plantation Caulis et Folium Brassicae junceae, only plants a Caulis et Folium Brassicae junceae in each flowerpot.
7. a kind of phyllotreta striolata biological control method using Caulis et Folium Brassicae junceae as bait crop according to claim 1 to 6 any one, it is characterised in that described vegetable is Caulis et Folium Brassicae capitatae.
CN201610107888.8A 2016-02-26 2016-02-26 Striped flea beetle biological control method adopting leaf mustard as trapping crop Pending CN105724134A (en)

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Cited By (3)

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Publication number Priority date Publication date Assignee Title
CN107079749A (en) * 2017-05-11 2017-08-22 温州科技职业学院 The method of the chrysomelid class pest of Rumex control of weeds brassicaceous vegetable
CN108271592A (en) * 2018-03-29 2018-07-13 福建省农业科学院植物保护研究所 A kind of with lemon-grass is the phyllotreta striolata biological control method for walking quickly and keeping away plant
CN111316847A (en) * 2020-03-10 2020-06-23 广西特色作物研究院 Prevention and control method for phyllotreta striolata

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CN101513176A (en) * 2009-03-06 2009-08-26 广东省昆虫研究所 Raising method of phyllotreta striolata experimental population
CN104273002A (en) * 2014-10-05 2015-01-14 安徽科技学院 Method for preventing phyllotreta striolata fabricius from damaging little cabbages by using enclosing films

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107079749A (en) * 2017-05-11 2017-08-22 温州科技职业学院 The method of the chrysomelid class pest of Rumex control of weeds brassicaceous vegetable
CN107079749B (en) * 2017-05-11 2020-07-17 温州科技职业学院 Method for preventing and treating leaf beetle pests of cruciferous vegetables by virtue of Rumex weeds
CN108271592A (en) * 2018-03-29 2018-07-13 福建省农业科学院植物保护研究所 A kind of with lemon-grass is the phyllotreta striolata biological control method for walking quickly and keeping away plant
CN111316847A (en) * 2020-03-10 2020-06-23 广西特色作物研究院 Prevention and control method for phyllotreta striolata
CN111316847B (en) * 2020-03-10 2021-06-25 广西特色作物研究院 Prevention and control method for phyllotreta striolata

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