CN105717090A - Integrated total internal reflecting microfluidic chip detection all-in-one machine - Google Patents
Integrated total internal reflecting microfluidic chip detection all-in-one machine Download PDFInfo
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- CN105717090A CN105717090A CN201610296065.4A CN201610296065A CN105717090A CN 105717090 A CN105717090 A CN 105717090A CN 201610296065 A CN201610296065 A CN 201610296065A CN 105717090 A CN105717090 A CN 105717090A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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Abstract
The invention discloses an integrated total internal reflecting microfluidic chip detection all-in-one machine. The all-in-one machine is composed of a mainframe box, a control box and a processing display, wherein the processing display is connected with an inverted microscope and a total internal reflecting optical receiver circuit in the mainframe box, and is further connected with a sample injector and a hot-cold compressor circuit in the control box. A light-guide fiber excitation source light source and an incident light angle calibration scale are installed in the mainframe box. Compared with the prior art, the all-in-one machine disclosed by the invention is capable of fast adjusting the total internal reflecting incident angle, the adaptability for chip detection is strong, the microfluidic chips in different types and different designs can be used. The machine is high in function integration degree, capable of realizing the temperature uniform and constant and humidity uniform and constant in the detection region, and meeting the detection and experiments under different temperature conditions.
Description
Technical field
The present invention relates to a kind of in-vitro diagnosis all-in-one, more specifically say the integrated total internal reflection micro-fluidic chip detection all-in-one of the one being belonging to the external diagnostic field of armarium.
Background technology
TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY is a kind of optical image technology emerging in recent years, and it utilizes evanescent field that total internal reflection produces to illuminate sample, thus causing the fluorogen in the thick optical layers of hundred nano-scale to be excited, the signal to noise ratio of fluorescence imaging is significantly high.The imaging device of this method is simple, very easily combines with other imaging technique, Detection Techniques.Successfully realize the even lower spatial resolution of 100nm at present.And at present micro-fluidic chip obtains and develops rapidly, and hinder the problem that the bottleneck that microflow control technique develops remains application aspect today.Total internal reflection optical detection technique is exactly one and meets the optical detective technology integrated with micro-fluidic chip.But, at present by also more rare for both integrated all-in-ones, lack the integrated detection equipment of both technology.
In order to cancer and cardiovascular and cerebrovascular disease, and the disposable checkout and diagnosis of the multiple protein factor of multiple great malignant disease, it is necessary to a kind of integrated total internal reflection micro-fluidic chip detection all-in-one.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, it is provided that a kind of integrated total internal reflection micro-fluidic chip detection all-in-one.
The object of the present invention is achieved like this: a kind of integrated total internal reflection micro-fluidic chip detection all-in-one, it is made up of mainframe box, control chamber and processing display, it is characterized in that: the vertical one side forward of main frame casing has opening, opening part to be provided with door, and back side wall has an interlayer cavity；Box house is divided into upper and lower two-layer by dividing plate, is provided with inverted microscope in lower floor's case, and disposing inverted microscope is meet a tractor serves several purposes purpose, can either detect with total internal reflection, can detect with inverted microscope again.Facing to observation window above inverted microscope camera lens；The installed above of dividing plate above observation window has sample stage；In sample stage left and right sides correspondence, 2 optical fibers excitation sources are installed, on the tank wall of 2 optical fibers excitation sources behinds, 1 incident angle of light calibration scale are respectively installed.So it is designed to fast tuning incident angle of light, quickly carries out total reflection detection.Fixed placement detection chip on sample stage, has total internal reflection optical receiver directly over detection chip.Being provided with perpendicular rail on the dorsal part box body wall of upper strata chamber, be also horizontally installed with traverse rod on perpendicular rail, traverse rod and perpendicular rail decussation are mutually perpendicular to；Total internal reflection optical receiver is connected with transverse arm by Universal rotary head, and transverse arm is fixed on perpendicular rail or traverse rod.So it is designed to meet total internal reflection optical receiver move vertically on perpendicular rail and fix；Total internal reflection optical receiver can also be met move left and right on traverse rod and fix；Certain angle can also be reversed by Universal rotary head, it is ensured that can quickly adjust position, make what total internal reflection detected to be smoothed out.On the dorsal part box body wall of upper strata chamber, also process the humidifier being provided with disjunctor and humidity sensor and humidity sensor controls fan, to have ensured that living cells detects the constant of required humidity.In the processing of mainframe box top, air cleaner is installed to be communicated with internal chamber by air cleaning ventilation filter hole.In the processing of mainframe box top, air cleaner is installed to communicate with chamber, has ensured the cleannes requirement of monitored area.It is control chamber on the right side of main frame casing, is connected with main frame casing.Processing display is placed on the table top of control chamber.Control chamber is internally installed calibrating gas bottle, sample injector, cold and hot compressor.Processing display is connected with the inverted microscope in mainframe box body, total internal reflection optical receiver circuit, is also connected with the sample injector in control chamber, cold and hot compression machine circuit.Processing display can control these devices.Cold and hot compressor is connected with interlayer cavity by cold and hot airduct；Interlayer cavity is connected with air cleaner by filtering holes.
Further, described humidity sensor controls fan and communicates with interlayer cavity, has ensured that the homogeneous temperature of chamber is with constant, humidity uniformly and constant.
Further, described main frame casing is by containment housing transparent, insulation material processing；It is transparent and airtight thermal insulating door thermally insulated door that described opening part is provided with door.This ensure that the temperature in case, humidity uniformly with constant.
Further, described inverted microscope is inverted fluorescence microscope, or inverted phase contrast microscope, so meets the detection needs of different sample.Described control chamber is independent case body, it is possible to fastening with mainframe box and couple or split, the height of control chamber is more than the 1/2 of mainframe box height.
The present invention has the advantage that relative to prior art.
1. can adjust total internal reflection angle of incidence, the strong adaptability to detection chip rapidly, it is possible to use variety classes and the micro-fluidic chip of different designs.
2. can detect with conventional microscopy again with total internal reflection techniques detection, it is achieved a tractor serves several purposes, the requirement of a multiple different protein factor of machine translocation.
3. combine the integration with all-in-one by total internal reflection detection technique, two kinds of technology of microfluidic chip technology, it is achieved that the homogeneous temperature in detection region with constant, humidity uniformly with constant.
Accompanying drawing explanation
Fig. 1 is the schematic front view of the present invention.
Fig. 2 is the left view schematic diagram of the present invention.
Wherein: 1. mainframe box；2. inverted microscope；3. observation window；4. dividing plate；5. sample stage；6. incident angle of light calibration scale；7. optical fibers excitation source；8. humidifier and humidity sensor；9. humidity sensor controls fan；10. total internal reflection optical receiver；11. air cleaner；12. perpendicular rail；13. traverse rod；14. detection chip；15. processing display；16. conduit；17. calibrating gas bottle；18. sample injector；19. control chamber；20. cold and hot compressor；21. cold and hot airduct；22. interlayer cavity；23. filtering holes；24. air cleaning ventilation filter hole；25. transverse arm；26. Universal rotary head.
Detailed description of the invention
Referring to Fig. 1-2, a kind of integrated total internal reflection micro-fluidic chip detection all-in-one, it is made up of mainframe box 1, control chamber 19 and processing display 15, it is characterised in that: the vertical one side forward of mainframe box 1 has opening, and opening part is provided with door.Back side wall has an interlayer cavity 22.Box house is divided into upper and lower two-layer by dividing plate 4, is provided with inverted microscope 2 in lower floor's case.Disposing inverted microscope is meet a tractor serves several purposes purpose, can either detect with total internal reflection, can detect with inverted microscope again.Facing to observation window 3 above inverted microscope 2 camera lens, the installed above of the dividing plate 4 above observation window 3 has sample stage 5.In sample stage 5 left and right sides, correspondence is provided with 2 optical fibers excitation sources 7, on the tank wall of 2 optical fibers excitation sources 7 behinds, is respectively provided with 1 incident angle of light calibration scale 6.Fixed placement detection chip 14 on sample stage 5, has total internal reflection optical receiver 10 directly over detection chip 14.Being provided with perpendicular rail 12 on the dorsal part box body wall of upper strata chamber, be also horizontally installed with traverse rod 13 on perpendicular rail 12, traverse rod 13 and perpendicular rail 12 decussation are mutually perpendicular to.Total internal reflection optical receiver 10 is connected with transverse arm 25 by Universal rotary head 26, and transverse arm 25 is fixed on perpendicular rail 12 or traverse rod 13.So it is designed to meet total internal reflection optical receiver 10 move vertically on perpendicular rail 12 and fix, total internal reflection optical receiver 10 can also be met move left and right on traverse rod 13 and fix, Universal rotary head 26 can also be passed through and reverse certain angle, guarantee quickly to adjust position, make what total internal reflection detected to be smoothed out.On the dorsal part box body wall of upper strata chamber, also process the humidifier being provided with disjunctor and humidity sensor 8 and humidity sensor controls fan 9, to have ensured that living cells detects the constant of required humidity.In the processing of mainframe box 1 top, air cleaner 11 is installed to be communicated with internal chamber by air cleaning ventilation filter hole 24.In the processing of mainframe box 1 top, air cleaner 11 is installed to communicate with chamber, has ensured the cleannes requirement of monitored area.It is control chamber 19 on the right side of mainframe box 1, is connected with mainframe box 1.Processing display 15 is placed on the table top of control chamber 19；Control chamber 19 is internally installed calibrating gas bottle 17, sample injector 18, cold and hot compressor 20.Processing display 15 is connected with the inverted microscope 2 of 1, total internal reflection optical receiver 10 circuit in mainframe box body, is also connected with the sample injector 18 in control chamber 19, cold and hot compressor 20 circuit.Processing display can control these devices being connected.Cold and hot compressor 20 is connected with interlayer cavity 22 by cold and hot airduct 21；Interlayer cavity 22 is connected with air cleaner 11 by filtering holes 23.
Further, described humidity sensor controls fan 9 and communicates with interlayer cavity 22, has ensured that the homogeneous temperature of chamber is with constant, humidity uniformly and constant.
Further, described mainframe box 1 is by containment housing transparent, insulation material processing；It is transparent and airtight thermal insulating door thermally insulated door that described opening part is provided with door.This ensure that the temperature in case, humidity uniformly with constant.
Further, described inverted microscope 2 is inverted fluorescence microscope, or inverted phase contrast microscope.So meet the detection needs of different sample.Described control chamber 19 is independent case body, it is possible to fastens with mainframe box 1 and couples or split, and the height of control chamber 19 is more than the 1/2 of mainframe box 1 height.
Claims (4)
1. an integrated total internal reflection micro-fluidic chip detection all-in-one, it is made up of mainframe box (1), control chamber (19) and processing display (15), it is characterized in that: the vertical one side forward of mainframe box (1) has opening, opening part to be provided with door, and back side wall has an interlayer cavity (22)；Box house is divided into upper and lower two-layer by dividing plate (4), is provided with inverted microscope (2) in lower floor's case, facing to observation window (3) above inverted microscope (2) camera lens；The installed above of dividing plate (4) of observation window (3) top has sample stage (5)；In sample stage (5) left and right sides correspondence, 2 optical fibers excitation sources (7) are installed, on the tank wall of 2 optical fibers excitation sources (7) behinds, 1 incident angle of light calibration scale (6) are respectively installed；The upper fixed placement detection chip (14) of sample stage (5), has total internal reflection optical receiver (10) directly over detection chip (14)；Being provided with perpendicular rail (12) on the dorsal part box body wall of upper strata chamber, be also horizontally installed with traverse rod (13) on perpendicular rail (12), traverse rod (13) and perpendicular rail (12) decussation are mutually perpendicular to；Total internal reflection optical receiver (10) is connected with transverse arm (25) by Universal rotary head (26)；Transverse arm (25) is fixed on perpendicular rail (12) or traverse rod (13)；On the dorsal part box body wall of upper strata chamber, also process the humidifier being provided with disjunctor and humidity sensor (8) and humidity sensor controls fan (9)；In the processing of main frame casing (1) top, air cleaner (11) is installed to be communicated with internal chamber by air cleaning ventilation filter hole (24)；Mainframe box (1) right side is control chamber (19), is connected with mainframe box (1)；Processing display (15) is placed on the table top of control chamber (19)；Control chamber (19) is internally installed calibrating gas bottle (17), sample injector (18), cold and hot compressor (20)；Processing display (15) is connected with the inverted microscope (2) of (1), total internal reflection optical receiver (10) circuit in mainframe box, is also connected with the sample injector (18) in control chamber (19), cold and hot compressor (20) circuit；Cold and hot compressor (20) is connected with interlayer cavity (22) by cold and hot airduct (21)；Interlayer cavity (22) is connected with air cleaner (11) by filtering holes (23).
2. the integrated total internal reflection micro-fluidic chip detection all-in-one of one according to claim 1, it is characterised in that: described humidity sensor controls fan (9) and communicates with interlayer cavity (22).
3. the integrated total internal reflection micro-fluidic chip detection all-in-one of one according to claim 1, it is characterised in that: described mainframe box (1) is by containment housing transparent, insulation material processing；Described opening part is provided with door, and door is transparent and airtight thermal insulating door thermally insulated door.
4. the integrated total internal reflection micro-fluidic chip detection all-in-one of one according to claim 1, it is characterised in that: described inverted microscope (2) is inverted fluorescence microscope, or inverted phase contrast microscope；Described control chamber (19) is independent case body, it is possible to fastens with mainframe box (1) and couples or split, and the height of control chamber (19) is more than the 1/2 of mainframe box (1) height.
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| CN201610296065.4A CN105717090B (en) | 2016-05-08 | 2016-05-08 | A kind of integrated total internal reflection micro-fluidic chip detection all-in-one machine |
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Cited By (4)
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| CN106680258A (en) * | 2017-02-09 | 2017-05-17 | 重庆科技学院 | Total internal reflection detection workbench |
| CN108323183A (en) * | 2018-02-09 | 2018-07-24 | 深圳达闼科技控股有限公司 | Raman detection ancillary equipment, Raman detection terminal and method |
| CN110568203A (en) * | 2019-09-12 | 2019-12-13 | 重庆科技学院 | Application method of multi-channel fluorescence immunochromatographic microfluidic chip |
| CN111004721A (en) * | 2019-12-24 | 2020-04-14 | 南通大学 | Ultrathin visual temperature control device suitable for microscope online in-situ observation |
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| CN111004721A (en) * | 2019-12-24 | 2020-04-14 | 南通大学 | Ultrathin visual temperature control device suitable for microscope online in-situ observation |
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| CN105717090B (en) | 2018-07-31 |
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