CN210719410U - Micro-fluidic chip temperature measurement imaging device - Google Patents
Micro-fluidic chip temperature measurement imaging device Download PDFInfo
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- CN210719410U CN210719410U CN201921708658.2U CN201921708658U CN210719410U CN 210719410 U CN210719410 U CN 210719410U CN 201921708658 U CN201921708658 U CN 201921708658U CN 210719410 U CN210719410 U CN 210719410U
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Abstract
The utility model provides a micro-fluidic chip temperature measurement imaging device, which comprises a near-infrared laser source for emitting a near-infrared laser beam, a semi-reflecting and semi-transmitting mirror for reflecting the near-infrared laser beam, an objective lens for collecting light emitted by upconversion nanoparticles in a micro-fluidic chip, and a camera for photographing and imaging; near-infrared laser source, half reflection and half transmission mirror, camera, objective all set up in the casing, and objective, half reflection and half transmission mirror, camera are on same vertical line, and set up filter set between half reflection and half transmission mirror and camera, and near-infrared laser source and half reflection and half transmission mirror still are provided with the temperature monitoring device of monitoring near-infrared laser source in the casing on same water flat line. According to the scheme, a method of irradiating upconversion nanoparticles by using a near-infrared laser beam is adopted, light emitted by the upconversion nanoparticles is collected by means of optical imaging, the temperature distribution condition of the microfluidic chip in a certain space range is obtained, the microfluidic chip does not need to be contacted, and the influence on the microfluidic chip is avoided.
Description
Technical Field
The utility model relates to a be used for micro-fluidic chip field, carry out non-contact's temperature measurement image device to micro-fluidic chip, obtain the temperature distribution of the inside local area within range of micro-fluidic chip, provide the foundation for physics, chemical reaction process in the analysis micro-fluidic chip passageway.
Background
The Microfluidic chip is also called a micro total analysis system because it has high integration and can complete various functions such as sampling, diluting, reagent adding, reaction, separation, detection and the like on one chip. At present, the microfluidic technology is applied to the fields of efficient screening, environmental monitoring, clinical monitoring, space microorganisms, field analysis, efficient sequencing and the like. The local temperature change in the microfluidic channel reveals the physical and chemical reaction processes occurring in the channel. Because the micro-fluidic chip has a small size and the diameter of the channel is tens to hundreds of micrometers, the micro-fluidic chip has high requirements on the spatial resolution of temperature detection. The temperature imaging space resolution of the traditional infrared temperature imager is low, and the temperature difference of two positions below a millimeter scale cannot be distinguished. Therefore, a technique for monitoring the temperature of the microfluidic chip without contact is needed.
Disclosure of Invention
The scheme adopts a method of irradiating upconversion nanoparticles by using a near-infrared laser beam, collects light emitted by the upconversion nanoparticles by means of optical imaging, obtains the temperature distribution condition of the microfluidic chip within a certain space range, does not need to contact the microfluidic chip, and avoids the influence on the microfluidic chip.
The scheme is realized by the following technology: a temperature measurement imaging device of a microfluidic chip comprises a near-infrared laser source for emitting a near-infrared laser beam, a half-reflecting and half-transmitting mirror for reflecting the near-infrared laser beam, an objective lens for collecting light emitted by upconversion nanoparticles in the microfluidic chip, and a camera for photographing and imaging; near-infrared laser source, half reflection and half transmission mirror, camera, objective all set up in the casing, and objective, half reflection and half transmission mirror, camera are on same vertical line, and set up filter set between half reflection and half transmission mirror and camera, and near-infrared laser source and half reflection and half transmission mirror still are provided with the temperature monitoring device of monitoring near-infrared laser source in the casing on same water flat line. Near-infrared laser beam that near-infrared laser source sent is reflected on the micro-fluidic chip by the half reflection semi-transparent mirror, and last conversion nanoparticle on the micro-fluidic chip sends light and passes the half reflection semi-transparent mirror through the collection of lens, gets into the camera formation of image after passing through the filter group again, can save the computer after the formation of image on, has temperature monitoring device, can monitor the temperature of near-infrared laser source, avoids the high temperature, has changed the air density in the casing for light takes place the refraction.
The nano-particles with the up-conversion luminescence property can emit visible light under the irradiation of external near-infrared laser beams, and the luminescence property of the nano-particles is changed along with the change of the temperature of the particles, so that the temperature change information of the positions of the nano-particles can be obtained.
The laser beam of the near-infrared laser source and the half-reflecting and half-transmitting mirror are arranged at an angle of 45 degrees. Therefore, after being reflected, the near-infrared laser beam irradiates the microfluidic chip through the objective lens, and the near-infrared ray emitted by the near-infrared laser source is the near-infrared ray with the wavelength of 980 nm. The excitation waveband has small damage to biological tissues, can not influence the activity of biological cells and protein molecules possibly existing in a channel of the microfluidic chip, and can not excite fluorescent substances possibly existing in the channel at the near infrared wavelength.
The upconversion nanoparticles are ytterbium (Yb3+) and erbium (Er3+) co-doped yttrium sodium fluoride (NaYF 4) crystal particles. Mixing NaYF 4: yb3+, Er3+, PDMS and a curing agent are uniformly mixed according to the weight ratio required by actual use, gas in the mixture is removed under the vacuum condition, and then the mixture is used for preparing a microfluidic chip with a certain function, so that the up-conversion nanoparticles are distributed in a chip base of the microfluidic chip.
The temperature monitoring device comprises a patch temperature sensor arranged on the near-infrared laser source, the patch temperature sensor is connected with a controller, and the controller is connected with the fan. The controller can adopt a commonly used controller, such as a single chip microcomputer, an aluminum alloy radiating fin is arranged on the shell, the front end of the aluminum alloy radiating fin is sleeved on the near-infrared laser source, the rear portion of the aluminum alloy radiating fin is arranged outside the shell, and the fan is fixed on the aluminum alloy radiating fin outside the shell. The paster temperature sensor sends the temperature information of the near-infrared laser source to the controller, and if the temperature exceeds a set threshold value, the controller controls the fan to start to cool.
Drawings
Fig. 1 is a schematic structural diagram of an embodiment of the present invention.
In the figure, 1 is a shell, 2 is a near-infrared laser source, 3 is a half-reflecting and half-transmitting mirror, 4 is an objective lens, 5 is a filter set, 6 is a camera, 7 is a computer, 8 is a fan, 9 is an aluminum alloy radiating fin, 10 is a patch temperature sensor, and 11 is a microfluidic chip.
Detailed Description
In order to more clearly illustrate the technical solution of the present invention, the technical solution of the present invention is described below with reference to the accompanying drawings. Spatially relative terms such as "under …", "below", "lower", "above", "over", and the like, as may be used herein for ease of description, describe one element or feature's relationship to another element or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if the device in the figures is turned over, elements described as "below" or "beneath" other elements or features would then be oriented "above" the other elements or features. Thus, the exemplary term "below" can encompass both an orientation of above and below. The device may be otherwise oriented (rotated 90 degrees or at other orientations) and the spatially relative terms used herein should be interpreted accordingly.
The attached drawing shows that the microfluidic chip temperature measurement imaging device comprises a near-infrared laser source 2 used for emitting a near-infrared laser beam, a half-reflecting half-transparent mirror 3 used for reflecting the near-infrared laser beam 2, and an objective 4 used for collecting light emitted by up-conversion nanoparticles in a microfluidic chip 11, wherein in the drawing, an original point in the microfluidic chip is the up-conversion nanoparticles, a camera 6 used for photographing and imaging is provided, and the up-conversion nanoparticles are ytterbium (Yb3+), erbium (Er3+) co-doped yttrium sodium fluoride (NaYF 4) crystal particles. Near-infrared laser source 2, the semi-reflecting and semi-transmitting mirror 3, camera 6, objective 4 all sets up in casing 1, casing 1 is opaque, objective 4, the semi-reflecting and semi-transmitting mirror 3, camera 6 is on same vertical line, and set up filter group 5 between the semi-reflecting and semi-transmitting mirror 3 and camera 6, near-infrared laser source 2 and semi-reflecting and semi-transmitting mirror 3 are on same horizontal line, the laser beam of near-infrared laser source 2 becomes 45 degrees angle settings with the semi-reflecting and semi-transmitting mirror 3, the near-infrared that near-infrared laser source 2 transmitted is the near-infrared of 980nm wavelength. A temperature monitoring device for monitoring the near-infrared laser source 2 is also arranged in the shell 1.
The temperature monitoring device comprises a patch temperature sensor 10 arranged on the near-infrared laser source 2, the patch temperature sensor 10 is connected with a controller, and the controller is connected with the fan 8. The aluminum alloy radiating fins 9 are arranged on the shell 1, the front ends of the aluminum alloy radiating fins 9 are sleeved on the near-infrared laser source 2, the rear portions of the aluminum alloy radiating fins 9 are arranged outside the shell 1, and the fan 8 is fixed on the aluminum alloy radiating fins 9 outside the shell 1.
The embodiments described above are only a part of the embodiments of the present invention, and not all of them. All other embodiments that can be derived by one of ordinary skill in the art from the detailed description given herein without any creative effort shall fall within the scope of protection of the present patent.
Claims (6)
1. The utility model provides a micro-fluidic chip temperature measurement image device which characterized by: the device comprises a near-infrared laser source for emitting a near-infrared laser beam, a half-reflecting and half-transmitting mirror for reflecting the near-infrared laser beam, an objective lens for collecting light rays emitted by upconversion nanoparticles in a microfluidic chip, and a camera for photographing and imaging; near-infrared laser source, half anti-semi-transparent mirror, camera, objective all set up in the casing, objective, half anti-semi-transparent mirror, camera are on same vertical line, and set up filter set between half anti-semi-transparent mirror and camera, near-infrared laser source and half anti-semi-transparent mirror still are provided with the temperature monitoring device of monitoring near-infrared laser source in the casing on same water flat line.
2. The micro-fluidic chip temperature measurement imaging device according to claim 1, wherein: the laser beam of the near-infrared laser source and the half-reflecting and half-transmitting mirror are arranged at an angle of 45 degrees.
3. The micro-fluidic chip temperature measurement imaging device according to claim 1, wherein: the up-conversion nano particles are ytterbium (Yb3+) and erbium (Er3+) co-doped yttrium sodium fluoride (NaYF 4) crystal particles.
4. The micro-fluidic chip temperature measurement imaging device according to claim 1, wherein: the temperature monitoring device comprises a patch temperature sensor arranged on the near-infrared laser source, the patch temperature sensor is connected with a controller, and the controller is connected with the fan.
5. The micro-fluidic chip temperature measurement imaging device according to claim 4, wherein: the shell is provided with an aluminum alloy radiating fin, the front end of the aluminum alloy radiating fin is sleeved on the near-infrared laser source, the rear portion of the aluminum alloy radiating fin is arranged outside the shell, and the fan is fixed on the aluminum alloy radiating fin outside the shell.
6. The micro-fluidic chip temperature measurement imaging device according to claim 1 or 5, wherein: the near infrared ray emitted by the near infrared laser source is the near infrared ray with the wavelength of 980 nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921708658.2U CN210719410U (en) | 2019-10-13 | 2019-10-13 | Micro-fluidic chip temperature measurement imaging device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201921708658.2U CN210719410U (en) | 2019-10-13 | 2019-10-13 | Micro-fluidic chip temperature measurement imaging device |
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| CN210719410U true CN210719410U (en) | 2020-06-09 |
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| CN201921708658.2U Expired - Fee Related CN210719410U (en) | 2019-10-13 | 2019-10-13 | Micro-fluidic chip temperature measurement imaging device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113433042A (en) * | 2021-06-25 | 2021-09-24 | 国家纳米科学中心 | Nanoparticle detection microfluidic chip and application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113433042A (en) * | 2021-06-25 | 2021-09-24 | 国家纳米科学中心 | Nanoparticle detection microfluidic chip and application |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200609 Termination date: 20201013 |