CN106520114A - Preparation method of fluorogold nano-cluster probe and application thereof - Google Patents
Preparation method of fluorogold nano-cluster probe and application thereof Download PDFInfo
- Publication number
- CN106520114A CN106520114A CN201610835262.9A CN201610835262A CN106520114A CN 106520114 A CN106520114 A CN 106520114A CN 201610835262 A CN201610835262 A CN 201610835262A CN 106520114 A CN106520114 A CN 106520114A
- Authority
- CN
- China
- Prior art keywords
- gold nano
- fluorescence
- nano cluster
- solution
- cluster probe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/58—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing copper, silver or gold
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
Abstract
The invention provides a preparation method of a fluorogold nano-cluster probe and application thereof and belongs to the field of a fluorescent nano material. The fluorogold nano-cluster probe is prepared in an alkaline environment on the basis of a 'one pot method' by taking silk fibroin as template and a protective agent, is uniform in size and high in stability and can respond to glutaraldehyde (GA). The method has the advantages of extensive and easily acquired raw materials, lower cost, simple operation and good repeatability. The prepared fluorogold nano-cluster probe has excellent water solubility, high stability and applicability in detecting GA in a practical water sample.
Description
Technical field
The present invention relates to fluorescent nano material preparing technical field, and in particular to a kind of system of fluorescence gold nano cluster probe
Preparation Method, and prepared probe can be used for detect actual water sample in glutaraldehyde.
Background technology
Metal nanometer cluster is typically referred to by the new fluorescence nano material of the molecular class of several to dozens of metal raws
Material.It has strong, the anti-light Bleachability good and high quantum yield of strong fluorescent emission, good biocompatibility, stability etc. excellent
Put and enjoy the concern of numerous researchers.In metal nanometer cluster, most study is gold nanoclusters, due to its water solublity
Good, toxicity is low, light stability is strong and luminous efficiency is high etc. it is excellent the characteristics of, be widely used in detection ion, biological micromolecule,
In terms of biomarker and imaging.
Glutaraldehyde (GA) is a kind of colourless, oiliness and noninflammable liquid at room temperature.Being of wide application for it is general, bag
Include sterilizer of fixative and medical apparatus and instruments of leather industry etc..But GA steam expose in atmosphere, can cause skin,
Respiratory tract and ocular allergies, and as it is in clinical and environmentally importance, we expect, therefore, that a kind of letter can be looked for
The good method of single, sensitivity and selectivity is detecting GA.
Biomacromolecule such as peptide and protein, with good biocompatibility, itself possesses various biological functions, easily
In the functionalization for realizing gold nano cluster, also it is commonly used for synthesizing the good template of fluorescence gold nano cluster probe.Document (Water-
soluble gold nanoclusters prepared by protein-ligand interaction as
fluorescent probe for real-time assay of pyrophosphatase activity,H.H.Deng,
F.F.Wang,X.Q.Shi,H.P.Peng,A.L.Liu,X.H.Xia,W.Chen,Biosensors and
83 (2016) 1-8 of Bioelectronics), with a kind of business-like common protein ----bovine serum albumin, add 3- mercaptos
Base propanoic acid has synthesized high luminous gold nano cluster, to Fe as template3+There is response, add PPi recover can its fluorescence.
But, the method building-up process needs two kinds of templates, relatively costly, therefore we synthesize simple in the urgent need to a kind of, low cost
Honest and clean new method.
The content of the invention
Present invention aim at providing a kind of preparation method of fluorescence gold nano cluster probe, the method is simple to operate, instead
Mild condition is answered, gained fluorescence gold nano cluster probe particle diameter is less, and good dispersion can be used for the detection of GA in actual water sample.
For achieving the above object, the preparation method of a kind of fluorescence gold nano cluster probe that the present invention is provided, step include：
(1) Bombyx bombycis are cut into into 1-2cm2Small pieces, Bombyx bombycis small pieces are placed in 0.01-0.03mol/L sodium carbonate liquors,
Degumming 0.5-2h is heated at 90-110 DEG C, and fibroin albumen is obtained；
(2) the fibroin albumen deionized water for obtaining step (1) is cleaned 2-4 time, in CaCl2:Water:Ethanol=1:8:2
Mixed solution in, heat 1-3h at 70-90 DEG C, dissolve fibroin albumen；
(3) silk fibroin protein solution obtained to step (2) is cooled to room temperature, filters, and dialyse 48h, is placed in standby in 4 DEG C of refrigerators
With；
(4) silk fibroin protein solution that step (3) is obtained is measured, is stirred continuously down, 0.5- is added in silk fibroin protein solution
1.5mmol/L gold chlorides, continuing stirring makes both fully mix, and chlorauric acid solution is 1- with the volume ratio of silk fibroin water solution
4:1；
(5) sodium hydroxide solution that 60 μ L concentration are 1mol/L is added in the mixed solution obtained to step (4), in 25-
Continue stirring 4-10h at 95 DEG C；
(6) mixed solution for obtaining step (5) finally gives fluorescence copper nanocluster probe solution through centrifugation.
In step (1), the concentration of sodium carbonate liquor is preferably 0.02mol/L.
In step (1), Bombyx bombycis small pieces preferably heat 1h at 100 DEG C with sodium carbonate liquor.
Fibroin albumen deionized water after the degumming obtained in step (2) is cleaned preferably 3 times, in CaCl2:Water：Ethanol
=1:8:In 2 mixed solution, preferably 2h is heated at 80 DEG C so as to dissolve.
In step (3), dialysis is with the bag filter that molecular cut off is 8000-14000Da.
The concentration of the chlorauric acid solution in step (4) is preferably 1mmol/L.
Chlorauric acid solution in step (4) is 2 with the volume ratio of silk fibroin water solution:1.
In step (5), preferably 8h is stirred at 37 DEG C.
In step (6), centrifugation is that 10min is centrifuged with 13000r/min rotating speeds.
Fluorescence gold nano cluster probe prepared by the inventive method can be used for the GA in detection actual water sample.
Compared with prior art, it is an advantage of the current invention that：
(1) with fibroin albumen as template, raw material is extensively easy to get, environmental protection, and preparation method is simple, with low cost.
(2) obtained in, fluorescence gold nano cluster probe has good blue luminescent properties, uses it for building actual water
The sensing system of GA is detected in sample, can avoid the interference of other Organic substances.Fluorescence gold nano cluster probe prepared by the present invention
GA in detectable actual water sample.
(3) obtained in, fluorescence gold nano cluster probe size is little, light stability is strong, toxic and side effects are little, good water solubility, fluorescence
Intensity is high, has broad application prospects in fields such as bio-imaging, biomarkers.
Description of the drawings
Fig. 1 is the mechanism of action schematic diagram of fluorescence gold nano cluster probe prepared by the present invention
It is ultraviolet for 365nm in daylight lamp (1) and wavelength respectively that Fig. 2 prepares fluorescence gold nano cluster probe solution for the present invention
Photo under lamp (2) irradiation
Fig. 3 is fluorescence-ultraviolet figure that the present invention prepares fluorescence gold nano cluster probe solution, and in figure, a is inhaled for ultraviolet-visible
Spectrogram is received, b is fluorescence spectra
Fig. 4 is the change of fluorescence peak intensity when fluorescence gold nano cluster probe solution prepared by the present invention adds different Organic substances
Change
Solution after the fluorescence gold nano cluster probe solution and addition GA of Fig. 5 present invention preparations is with its fluorescence of the change of pH
The change of peak intensity
Fluorescence gold nano cluster probe prepared by Fig. 6 present invention adds solution its fluorescence peak intensity over time after GA
The change of degree
Fig. 7 the present invention prepare fluorescence gold nano cluster probe solution with ionic strength (concentration of Sodium Chloride) change its
The change of fluorescence peak intensity
The change of fluorescence gold nano cluster probe solution prepared by Fig. 8 present invention with its fluorescence peak intensity of the change of GA concentration
Fig. 9 (a) is that fluorescence gold nano cluster probe solution and concentration range are the linear relationship between 0-80 μM of GA
Fig. 9 (b) is that fluorescence gold nano cluster probe solution and concentration range are the linear relationship between 0-10 μM of GA
Fig. 9 (c) is that fluorescence gold nano cluster probe solution and concentration range are the linear relationship between 20-80 μM of GA
Specific embodiment
The present invention is, with fibroin albumen as template, in alkaline environment, to prepare fluorescence gold nano cluster by " one kettle way "
Probe solution, and for the detection of GA in actual water sample.Accompanying drawing is combined below by embodiment, and the invention will be further described.
Embodiment 1
The preparation of the fluorescence gold nano cluster probe with fibroin albumen as template：
(1) degumming silkworm cocoons prepare fibroin albumen：Bombyx bombycis are cut into into 1-2cm2Small pieces, Bombyx bombycis small pieces are placed in into 0.02mol/
In L sodium carbonate liquors, 1h is heated at 100 DEG C；
(2) the fibroin albumen deionized water after the degumming for obtaining step (1) is cleaned 3 times, in CaCl2:Water:Ethanol=
1:8:In 2 mixed solution, 2h is heated at 80 DEG C so as to dissolve；
(3) silk fibroin protein solution obtained to step (2) is cooled to room temperature, filters, and dialyse 48h, is placed in standby in 4 DEG C of refrigerators
With；
(4) silk fibroin protein solution that step (3) is obtained is measured, is stirred continuously down, added in silk fibroin protein solution
1mmol/L gold chlorides, continuing stirring makes both fully mix, and chlorauric acid solution is 2 with the volume ratio of silk fibroin water solution:1；
(5) sodium hydroxide solution that 60 μ L concentration are 1mol/L is added in the mixed solution obtained to step (4), at 37 DEG C
It is lower to continue stirring 8h；
(6) solution for obtaining step (5) is centrifuged 10min with 13000r/min rotating speeds, finally gives fluorogold nanoclusters
Aggregate probe solution.
The mechanism of action schematic diagram of the fluorescence gold nano cluster probe of preparation is shown in Fig. 1.
The fluorescence gold nano cluster probe solution of preparation is the photograph under 365nm ultra violet lamps in daylight lamp and wavelength respectively
Piece is shown in Fig. 2, and in figure, 1 is picture of the fluorescence gold nano cluster probe solution under daylight light irradiation, and color is pink colour, and 2 is wavelength
For the picture under 365nm ultra violet lamps, color is blue.
Additionally, fluorescence-ultraviolet the figure of the fluorescence gold nano cluster probe solution for preparing is shown in Fig. 3, wherein fluorogram (b) shows
, under the conditions of fixed excitation wavelength is 331nm, emission peak positions are in 420nm or so for the fluorescence gold nano cluster probe of preparation.
Embodiment 2
The impact experiment of the fluorescence peak intensity of the fluorescence gold nano cluster probe solution that Organic substance is prepared to embodiment 1：
Concentration is configured to respectively for 800 μ with the BR buffer solution and formaldehyde, acetaldehyde, methanol, ethanol, acetone of pH=8
mol·L-1Solution, GA is configured to into 80 μm of ol L-1Solution, the fluorescence gold nano for respectively being prepared by 0.1mL embodiments 1
Cluster probe solution is added in the above-mentioned solution containing different Organic substances of 0.9mL.Fixed excitation wavelength is 331nm, at room temperature
Fluorescence spectrum detection is carried out, according to the fluorescence peak intensity of 420nm or so, detection Organic substance is to fluorescence gold nano cluster probe solution
Fluorescence peak intensity impact.
Fig. 4 is shown in impact of the Organic substance to the fluorescence peak intensity of fluorescence gold nano cluster probe solution：In the case where 331nm is excited,
From fluorescence intensity F of the fluorescence gold nano cluster probe solution for not containing machine thing0Visit with the fluorescence gold nano cluster containing Organic substance
The ratio of fluorescence intensity F of pin solution draws：GA changes are maximum, and other Organic substance changes are relatively small, illustrate present invention preparation
Fluorescence gold nano cluster probe solution being capable of qualitative detection GA.
Embodiment 3
The fluorescence gold nano cluster probe solution that pH value is prepared to embodiment 1 is molten with the fluorescence gold nano cluster after addition GA
The impact experiment of the fluorescence intensity of liquid：
Fluorescence gold nano after the fluorescence gold nano cluster probe solution for respectively being prepared by 100 μ L embodiments 1 and addition GA
Cluster solution is added separately in the BR buffer solution of 900 μ L difference pH value, and fixed excitation wavelength is 331nm, is entered at room temperature
Row fluorescence spectrum detects that, according to the fluorescence peak intensity of 420nm or so, detection pH value is to fluorescence gold nano cluster probe solution and adds
Enter the impact of the fluorescence intensity of the fluorescence gold nano cluster solution after GA.
Fluorescence intensity of the pH value to the fluorescence gold nano cluster solution after fluorescence gold nano cluster probe solution and addition GA
Impact see Fig. 5：In the case where 331nm is excited, the fluorescence gold nano cluster after fluorescence gold nano cluster probe solution and addition GA is molten
, when pH is 8, fluorescence intensity change is larger for the fluorescence intensity of liquid, thus fluorescence gold nano cluster probe solution prepared by the present invention
Select pH=8.
Embodiment 4
The fluorescence gold nano cluster probe solution that time is prepared to embodiment 1 adds the impact experiment after GA：
After fluorescence gold nano cluster probe solution prepared by 100 μ L embodiments 1 adds GA, 100 μ L BR bufferings are added to
In solution (pH=8.0), then plus 800 μ L deionized waters, fixed excitation wavelength is 331nm, is carried out at room temperature glimmering in 0-10min
Light spectral detection, according to the fluorescence peak intensity of 420nm or so, fluorescence peak of the detection time to fluorescence gold nano cluster probe solution
The impact of intensity.Fig. 6 is shown in impact of the time to the fluorescence intensity of fluorescence gold nano cluster probe solution：In 10min, fluorogold
The fluorescence intensity of nanocluster probe is held essentially constant.
Embodiment 5
The impact experiment of the fluorescence intensity of the fluorescence gold nano cluster probe solution that ionic strength is prepared to embodiment 1：
Fluorescence gold nano cluster probe solution prepared by 100 μ L embodiments 1 is added to into 100 μ L BR buffer solution (pH=
8.0) in, then plus 800 μ L deionized waters, fixed excitation wavelength is 331nm, add variable concentrations sodium chloride solution (0～
300mmol/L), the fluorescence intensity according to 420nm or so, detects fluorescence of the ionic strength to fluorescence gold nano cluster probe solution
The impact of intensity.
Fig. 7 is shown in impact of the ionic strength to the fluorescence intensity of fluorescence gold nano cluster probe solution：In the case where 331nm is excited,
, in the range of the sodium chloride solution (0～300mmol/L) of variable concentrations, fluorescence intensity is basic for fluorescence gold nano cluster probe solution
It is constant, illustrate that the anti-ion interference of fluorescence gold nano cluster probe solution of present invention preparation is strong.
Embodiment 6
The experiment that fluorescence gold nano cluster probe solution prepared by embodiment 1 is detected to GA：
Fluorescence gold nano cluster probe solution prepared by embodiment 1 dilutes 10 times, takes the fluorogold nanoclusters after dilution
100 μ L of aggregate probe solution are added in solution of the 900 μ L containing GA, and fixed excitation wavelength is 331nm, carries out fluorescence light at room temperature
Spectrum detection, according to the fluorescence intensity of 420nm or so, detects impacts of the GA to the fluorescence intensity of gold nano cluster probe solution.
Fig. 8 is shown in impacts of the GA to the fluorescence intensity of fluorescence gold nano cluster probe solution：In the case where 331nm is excited, fluorogold
After the GA for adding variable concentrations, fluorescence intensity is gradually reduced nanocluster probe solution, and last fluorescence peak is substantially smooth-out；
Wherein 0-80 μM is the GA of 0,1,2,3,4,5,6,7,8,9,10,20,30,40,50,60,70,80 μm of ol/L to fluorogold respectively
The fluorescence spectra that nanocluster probe solution fluorescence intensity affects, illustrates that the fluorescence gold nano cluster probe of present invention preparation is molten
Liquid energy enough realizes the detection to GA.
Additionally, the change of the fluorescence intensity of the fluorescence gold nano cluster probe solution of present invention preparation is in line with the concentration of GA
Shown in sexual intercourse, such as Fig. 9 (a), the fluorescence intensity of gold nano cluster and the concentration of GA are linear into two sections.As shown in Fig. 9 (b), GA
Linear equation be F0/ F=0.976+0.07C (R2=0.994)；As shown in Fig. 9 (c), the linear equation of GA is F0/ F=
1.303+0.0247C(R2=0.994)
Embodiment 7
Fluorescence gold nano cluster probe solution prepared by embodiment 1 is used for the test experience of GA in actual water sample：
The fluorescence gold nano cluster probe solution for being used for the preparation of embodiment 1 using standard addition method is biological in actual water sample
The experiment of GA detection applications.As shown in table 1, the GA solution of 3,6,9 μm of ol/L is respectively with actual water sample compound concentration, will be implemented
Fluorescence gold nano cluster probe solution prepared by example 1 dilutes 10 times, takes the 100 μ L of fluorescence gold nano cluster probe solution after dilution
It is added separately in solution of the 900 μ L containing GA, fixed excitation wavelength is 331nm, carries out fluorescence spectrum detection at room temperature, and remembers
Record corresponding fluorescence intensity.
The response rate of GA in actual water sample is calculated using the linear equation in Fig. 9 (b).Multigroup parallel assay in this experiment
And the response rate of GA is calculated, as shown in table 1, illustrate that fluorescence gold nano cluster probe solution prepared by embodiment 1 can be used in water
The detection of GA in sample.
Table 1 is used for the detection of GA in actual water sample for fluorescence gold nano cluster probe solution prepared by the present invention
Claims (10)
1. a kind of preparation method of fluorescence gold nano cluster probe, it is characterised in that comprise the steps：
(1) Bombyx bombycis are cut into into 1-2cm2Small pieces, Bombyx bombycis small pieces are placed in 0.01-0.03mol/L sodium carbonate liquors, in 90-
Degumming 0.5-2h is heated at 110 DEG C, and fibroin albumen is obtained；
(2) the fibroin albumen deionized water for obtaining step (1) is cleaned 2-4 time, in CaCl2:Water:Ethanol=1:8:2 mixing
In solution, 1-3h is heated at 70-90 DEG C, dissolve fibroin albumen；
(3) silk fibroin protein solution obtained to step (2) is cooled to room temperature, filters, and dialyse 48h, is placed in standby in 4 DEG C of refrigerators；
(4) silk fibroin protein solution that step (3) is obtained is measured, is stirred continuously down, 0.5- is added in silk fibroin protein solution
1.5mmol/L gold chlorides, continuing stirring makes both fully mix, and chlorauric acid solution is 1- with the volume ratio of silk fibroin water solution
4:1；
(5) sodium hydroxide solution that 60 μ L concentration are 1mol/L is added in the mixed solution obtained to step (4), at 25-95 DEG C
It is lower to continue stirring 4-10h；
(6) mixed solution for obtaining step (5) finally gives fluorescence gold nano cluster probe solution through centrifugation.
2. a kind of preparation method of fluorescence gold nano cluster probe as claimed in claim 1, it is characterised in that described step
(1) in, the concentration of sodium carbonate liquor is 0.02mol/L.
3. a kind of preparation method of fluorescence gold nano cluster probe as claimed in claim 1, it is characterised in that described step
(1) in, Bombyx bombycis small pieces heat 1h with sodium carbonate liquor at 100 DEG C.
4. a kind of preparation method of fluorescence gold nano cluster probe as claimed in claim 1, it is characterised in that described step
(2) the fibroin albumen deionized water after the degumming obtained in is cleaned 3 times, in CaCl2:Water：Ethanol=1:8:2 mixed solution
In, 2h is heated at 80 DEG C so as to dissolve.
5. a kind of preparation method of fluorescence gold nano cluster probe as claimed in claim 1, it is characterised in that described step
(3) in, dialysis is with the bag filter dialysis that molecular cut off is 8000-14000Da.
6. a kind of preparation method of fluorescence gold nano cluster probe as claimed in claim 1, it is characterised in that described step
(4) concentration of the chlorauric acid solution in is 1mmol/L.
7. a kind of preparation method of fluorescence gold nano cluster probe as claimed in claim 1, it is characterised in that described step
(4) gold chloride in is 2 with the volume ratio of silk fibroin water solution:1.
8. a kind of preparation method of fluorescence gold nano cluster probe as claimed in claim 1, it is characterised in that described step
(5), in, 8h is stirred at 37 DEG C.
9. a kind of preparation method of fluorescence gold nano cluster probe as claimed in claim 1, it is characterised in that described step
(6) in, centrifugation is that 10min is centrifuged with 13000r/min rotating speeds.
10. the fluorescence gold nano cluster probe that prepared by the either method as described in claim 1-9 is used for the detection of glutaraldehyde in water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610835262.9A CN106520114B (en) | 2016-09-20 | 2016-09-20 | A kind of preparation method and application of fluorescence gold nano cluster probe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610835262.9A CN106520114B (en) | 2016-09-20 | 2016-09-20 | A kind of preparation method and application of fluorescence gold nano cluster probe |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106520114A true CN106520114A (en) | 2017-03-22 |
| CN106520114B CN106520114B (en) | 2018-10-16 |
Family
ID=58343859
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610835262.9A Expired - Fee Related CN106520114B (en) | 2016-09-20 | 2016-09-20 | A kind of preparation method and application of fluorescence gold nano cluster probe |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106520114B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107607503A (en) * | 2017-08-30 | 2018-01-19 | 王延敏 | The assay method of trace copper ion in water environment |
| CN108031859A (en) * | 2017-11-29 | 2018-05-15 | 上海纳米技术及应用国家工程研究中心有限公司 | Method for synthesizing gold nanoclusters and products thereof and application |
| CN108031857A (en) * | 2017-11-21 | 2018-05-15 | 东华大学 | A kind of preparation method for the gold nano cluster for sending out red fluorescence |
| CN109705841A (en) * | 2018-12-24 | 2019-05-03 | 山西大学 | A kind of transferrins is the gold nano cluster and its preparation method and application of template |
| CN113201327A (en) * | 2021-04-27 | 2021-08-03 | 武汉理工大学 | Gold-silver alloy nanocluster and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007018551A2 (en) * | 2004-09-13 | 2007-02-15 | Intel Corporation | External modification of composite organic inorganic nanoclusters |
| CN105199718A (en) * | 2015-11-04 | 2015-12-30 | 山西大学 | Red fluorescent gold/copper nanocluster alloy as well as preparation method and application thereof |
| CN105345025A (en) * | 2015-11-04 | 2016-02-24 | 山西大学 | Green fluorescence silver nanocluster probe and preparing method and application thereof |
| CN105713602A (en) * | 2016-01-21 | 2016-06-29 | 山西大学 | Preparation method and application of fluorescence copper nanocluster probe |
| CN105839404A (en) * | 2016-04-11 | 2016-08-10 | 武汉纺织大学 | A preparing method of a silk fabric with adjustable fluorescence colors |
-
2016
- 2016-09-20 CN CN201610835262.9A patent/CN106520114B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007018551A2 (en) * | 2004-09-13 | 2007-02-15 | Intel Corporation | External modification of composite organic inorganic nanoclusters |
| CN105199718A (en) * | 2015-11-04 | 2015-12-30 | 山西大学 | Red fluorescent gold/copper nanocluster alloy as well as preparation method and application thereof |
| CN105345025A (en) * | 2015-11-04 | 2016-02-24 | 山西大学 | Green fluorescence silver nanocluster probe and preparing method and application thereof |
| CN105713602A (en) * | 2016-01-21 | 2016-06-29 | 山西大学 | Preparation method and application of fluorescence copper nanocluster probe |
| CN105839404A (en) * | 2016-04-11 | 2016-08-10 | 武汉纺织大学 | A preparing method of a silk fabric with adjustable fluorescence colors |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107607503A (en) * | 2017-08-30 | 2018-01-19 | 王延敏 | The assay method of trace copper ion in water environment |
| CN108031857A (en) * | 2017-11-21 | 2018-05-15 | 东华大学 | A kind of preparation method for the gold nano cluster for sending out red fluorescence |
| CN108031859A (en) * | 2017-11-29 | 2018-05-15 | 上海纳米技术及应用国家工程研究中心有限公司 | Method for synthesizing gold nanoclusters and products thereof and application |
| CN109705841A (en) * | 2018-12-24 | 2019-05-03 | 山西大学 | A kind of transferrins is the gold nano cluster and its preparation method and application of template |
| CN109705841B (en) * | 2018-12-24 | 2021-07-02 | 山西大学 | Gold nanocluster with transferrin as template and preparation method and application thereof |
| CN113201327A (en) * | 2021-04-27 | 2021-08-03 | 武汉理工大学 | Gold-silver alloy nanocluster and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106520114B (en) | 2018-10-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106520114B (en) | A kind of preparation method and application of fluorescence gold nano cluster probe | |
| CN105713602B (en) | A kind of preparation method and application of fluorescence copper nanocluster probe | |
| CN103357886B (en) | A kind of preparation method of the noble metal nano cluster for fluorescent optical sensor | |
| Wang et al. | Synthesis and Applications of Red‐Emissive Carbon Dots | |
| CN105199716B (en) | 3 mercaptopropionic acid bovine serum albumin(BSA) gold nano clusters and preparation method thereof | |
| CN103289684B (en) | Fluorescent silver nanocluster as well as preparation method and application thereof | |
| CN105345025B (en) | A kind of green fluorescence ag nano-cluster probe and its preparation method and application | |
| CN106892947B (en) | One kind containing the complex of iridium and its preparation method and application of (Hydrazinocarbonyl) ferrocene ligands | |
| CN107225255A (en) | A kind of red fluorescence ag nano-cluster probe and its preparation method and application | |
| CN105044060A (en) | Preparation and application of red fluorescent silver nanocluster | |
| CN113563351B (en) | Water-soluble ring-opening cucurbituril fluorescent probe and application thereof | |
| CN108918478A (en) | A kind of method of quantitative detection alpha-glucosidase activity | |
| CN100487074C (en) | Method for preparing small-particle-size water-soluble cadium selenide quantum dot and its use | |
| CN112444505B (en) | Intracellular detection method based on double-excitation-ratio up-conversion fluorescent probe | |
| CN111440608A (en) | Double-emission-ratio fluorescent probe and method for detecting copper ions | |
| Zhao et al. | Stable aqueous ZnO nanoparticles with green photoluminescence and biocompatibility | |
| CN109211862A (en) | A kind of preparation method and applications of red fluorescence copper nanocluster probe | |
| CN107138736A (en) | A kind of preparation method and applications of state of aggregation phosphorescence copper nano-cluster | |
| CN108732151B (en) | Preparation of luminescent gold nanoparticles with high-sensitivity optical response to volatile amine and rapid analysis and detection method thereof | |
| CN109794616A (en) | The preparation of the silver nanoclusters of sericin package and fluorescence probe | |
| CN108314636A (en) | A kind of polyaryl sulphur oscillation luminescent material and its preparation method and application | |
| CN105463616B (en) | A kind of cadmium telluride quantum dot/polylactic acid nano fiber fluorescence probe preparation method to chloramphenicol detection | |
| CN107151555A (en) | A kind of controllable luminous carbon nano-particles and preparation method and application | |
| CN106632088A (en) | Aggregation-induced emission probe based on quinazolinones compound as well as preparation method and application of aggregation-induced emission probe | |
| Jung et al. | High selective fluorescence imaging of cesium distribution in Arabidopsis using a bis (trihydroxyphenyl)-appended fluorescent probe with a turn-on system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181016 Termination date: 20210920 |