CN105713932B - 一种微生物发酵制备2,3-丁二醇的方法 - Google Patents
一种微生物发酵制备2,3-丁二醇的方法 Download PDFInfo
- Publication number
- CN105713932B CN105713932B CN201410730613.0A CN201410730613A CN105713932B CN 105713932 B CN105713932 B CN 105713932B CN 201410730613 A CN201410730613 A CN 201410730613A CN 105713932 B CN105713932 B CN 105713932B
- Authority
- CN
- China
- Prior art keywords
- fermentation
- glucose
- seed liquor
- culture
- lactobacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000855 fermentation Methods 0.000 claims abstract description 88
- 230000004151 fermentation Effects 0.000 claims abstract description 88
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 50
- 239000008103 glucose Substances 0.000 claims abstract description 47
- OWBTYPJTUOEWEK-UHFFFAOYSA-N butane-2,3-diol Chemical compound CC(O)C(C)O OWBTYPJTUOEWEK-UHFFFAOYSA-N 0.000 claims abstract description 39
- 238000000034 method Methods 0.000 claims abstract description 33
- 239000007788 liquid Substances 0.000 claims abstract description 30
- 241000588748 Klebsiella Species 0.000 claims abstract description 17
- 241000186660 Lactobacillus Species 0.000 claims abstract description 16
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 16
- 239000001963 growth medium Substances 0.000 claims abstract description 15
- 238000011534 incubation Methods 0.000 claims abstract description 13
- 238000010564 aerobic fermentation Methods 0.000 claims abstract description 8
- 239000002609 medium Substances 0.000 claims abstract description 7
- 230000000813 microbial effect Effects 0.000 claims abstract description 7
- 244000199866 Lactobacillus casei Species 0.000 claims description 12
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 12
- 229940017800 lactobacillus casei Drugs 0.000 claims description 12
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 10
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 239000001301 oxygen Substances 0.000 claims description 6
- 238000011218 seed culture Methods 0.000 claims description 5
- 241000607142 Salmonella Species 0.000 claims description 4
- 239000000758 substrate Substances 0.000 claims description 4
- 230000003698 anagen phase Effects 0.000 claims description 2
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 239000007789 gas Substances 0.000 claims 1
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 2
- 238000000746 purification Methods 0.000 abstract description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 239000012071 phase Substances 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000012467 final product Substances 0.000 description 6
- 238000000926 separation method Methods 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 5
- 235000019441 ethanol Nutrition 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 4
- 239000000796 flavoring agent Substances 0.000 description 4
- 238000010899 nucleation Methods 0.000 description 4
- 238000004321 preservation Methods 0.000 description 4
- ZWEHNKRNPOVVGH-UHFFFAOYSA-N 2-Butanone Chemical compound CCC(C)=O ZWEHNKRNPOVVGH-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 241000588747 Klebsiella pneumoniae Species 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229960004756 ethanol Drugs 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 235000019634 flavors Nutrition 0.000 description 3
- 239000007791 liquid phase Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- KAKZBPTYRLMSJV-UHFFFAOYSA-N Butadiene Chemical compound C=CC=C KAKZBPTYRLMSJV-UHFFFAOYSA-N 0.000 description 2
- QSJXEFYPDANLFS-UHFFFAOYSA-N Diacetyl Chemical group CC(=O)C(C)=O QSJXEFYPDANLFS-UHFFFAOYSA-N 0.000 description 2
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- -1 humidizer Substances 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000004014 plasticizer Substances 0.000 description 2
- 239000002243 precursor Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 235000020097 white wine Nutrition 0.000 description 2
- PKAUICCNAWQPAU-UHFFFAOYSA-N 2-(4-chloro-2-methylphenoxy)acetic acid;n-methylmethanamine Chemical compound CNC.CC1=CC(Cl)=CC=C1OCC(O)=O PKAUICCNAWQPAU-UHFFFAOYSA-N 0.000 description 1
- ROWKJAVDOGWPAT-UHFFFAOYSA-N Acetoin Chemical compound CC(O)C(C)=O ROWKJAVDOGWPAT-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 229920005830 Polyurethane Foam Polymers 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 239000004902 Softening Agent Substances 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- VVSAAKSQXNXBML-PHDIDXHHSA-N [(2R,3R)-3-acetyloxybutan-2-yl] acetate Chemical class CC(=O)O[C@H](C)[C@@H](C)OC(C)=O VVSAAKSQXNXBML-PHDIDXHHSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000009056 active transport Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000009194 climbing Effects 0.000 description 1
- 238000004939 coking Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 229960000935 dehydrated alcohol Drugs 0.000 description 1
- 238000006356 dehydrogenation reaction Methods 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 229910000388 diammonium phosphate Inorganic materials 0.000 description 1
- 150000002009 diols Chemical class 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000032050 esterification Effects 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013355 food flavoring agent Nutrition 0.000 description 1
- 239000002816 fuel additive Substances 0.000 description 1
- 239000002316 fumigant Substances 0.000 description 1
- 230000004153 glucose metabolism Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000000976 ink Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007269 microbial metabolism Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 238000005120 petroleum cracking Methods 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 239000011496 polyurethane foam Substances 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Inorganic materials [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229920003051 synthetic elastomer Polymers 0.000 description 1
- 239000005061 synthetic rubber Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
配方 | LB培养基(/L) | MRS基本培养(/L) | 发酵用培养(/L) |
Tryptone | 10g | 10g | — |
Yeast Extrac | 5g | 5g | — |
NaCl | 10g | — | — |
葡萄糖 | — | 20g | 100g |
醋酸钠 | — | 5g | — |
柠檬酸二铵 | — | 2g | — |
MgSO<sub>4</sub>•7H<sub>2</sub>O | — | 0.52g | 0.25g |
MnSO<sub>4</sub>•H<sub>2</sub>O | — | 0.28g | 0.001g |
吐温80 | — | 0.1g | — |
K<sub>2</sub>HPO<sub>4</sub>•3H<sub>2</sub>O | — | — | 13.7g |
KH<sub>2</sub>PO<sub>4</sub> | — | — | 2g |
(NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> | — | — | 6.6g |
(NH<sub>4</sub>)<sub>2</sub>HPO<sub>4</sub> | — | — | 3.3g |
FeSO<sub>4</sub>•7H<sub>2</sub>O | — | — | 0.05g |
ZnSO<sub>4</sub>•7H<sub>2</sub>O | — | — | 0.001g |
CaCl<sub>2</sub> | — | — | 0.01g |
EDTA | — | — | 0.05g |
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410730613.0A CN105713932B (zh) | 2014-12-05 | 2014-12-05 | 一种微生物发酵制备2,3-丁二醇的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410730613.0A CN105713932B (zh) | 2014-12-05 | 2014-12-05 | 一种微生物发酵制备2,3-丁二醇的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105713932A CN105713932A (zh) | 2016-06-29 |
CN105713932B true CN105713932B (zh) | 2019-08-06 |
Family
ID=56143475
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410730613.0A Active CN105713932B (zh) | 2014-12-05 | 2014-12-05 | 一种微生物发酵制备2,3-丁二醇的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105713932B (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108118073B (zh) * | 2016-11-29 | 2020-09-11 | 中国石油化工股份有限公司 | 一种以微藻为原料生产2,3-丁二醇的方法 |
CN108384766B (zh) * | 2018-06-11 | 2020-12-08 | 宁波天皓生物科技有限公司 | 一种利用微生物发酵制备葡萄糖氧化酶的方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101457211A (zh) * | 2008-08-04 | 2009-06-17 | 山东大学 | 一株肺炎克雷伯氏菌及其在制备2,3-丁二醇中的应用 |
-
2014
- 2014-12-05 CN CN201410730613.0A patent/CN105713932B/zh active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101457211A (zh) * | 2008-08-04 | 2009-06-17 | 山东大学 | 一株肺炎克雷伯氏菌及其在制备2,3-丁二醇中的应用 |
Non-Patent Citations (1)
Title |
---|
A two-stage,two-organism process for biohydrogen from glucose;Mark D. Redwood et al.;《Int. J. Hydrogen Energy》;20060930;第31卷(第11期);摘要部分 |
Also Published As
Publication number | Publication date |
---|---|
CN105713932A (zh) | 2016-06-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN100554405C (zh) | 一种生产l-乳酸的方法及其专用鼠李糖乳杆菌 | |
TWI494433B (zh) | 製造羧酸及/或醇的方法 | |
CN102559782B (zh) | 利用酪丁酸梭菌发酵甘蔗渣水解液生产丁酸的工艺 | |
CN101487029B (zh) | 一种微生物催化生产正丁酸的方法和装置 | |
CN105713932B (zh) | 一种微生物发酵制备2,3-丁二醇的方法 | |
CN106929548B (zh) | 一种采用米曲霉发酵产苹果酸的工艺 | |
CN101538592B (zh) | 用寇氏隐甲藻工业化发酵生产二十二碳六烯酸的方法 | |
CN102212451A (zh) | 基于微生物共培养的白酒黄水利用方法 | |
CN111019995B (zh) | 一种以丁香酚为底物发酵生成香兰素的方法 | |
CN102876760B (zh) | 枯草芽孢菌在高产腺苷且联产乙偶姻中的应用 | |
Patel et al. | Production of acetic acid from molasses by fermentation process | |
CN110283854B (zh) | 一种发酵培养基及其应用和利用三孢布拉霉菌发酵制备番茄红素的方法 | |
CN104611401B (zh) | 一种提高微生物发酵生产2,3‑丁二醇的方法 | |
CN104630301A (zh) | 一种发酵生产利普司他汀的方法 | |
CN102181490B (zh) | 一种生产柠檬酸的方法 | |
CN116426577A (zh) | 一种以氢氧化钙为中和剂结合co2脉冲反馈补料发酵生产丁二酸的方法 | |
CN102321683B (zh) | 采用发酵法制备富马酸发酵液及富马酸发酵液分离提取纯品富马酸的方法 | |
CN108690853B (zh) | 一种发酵生产丁醇的方法 | |
CN106906246A (zh) | 一种提高库德毕赤酵母高温乙醇发酵的方法 | |
CN108486173B (zh) | 一种α-酮戊二酸的制备方法 | |
CN105585430B (zh) | 一种从发酵液中提取2,3‑丁二醇的方法 | |
CN104404095B (zh) | 一种发酵生产l-亮氨酸的方法 | |
CN110982716B (zh) | 用于生产天然酪醇的菌株及天然酪醇的制备方法 | |
CN118240887B (zh) | 一种利用含铵离子溶液产油脂的方法 | |
CN105585432B (zh) | 一种从发酵液中分离提取2,3-丁二醇的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20230826 Address after: 100728 No. 22 North Main Street, Chaoyang District, Beijing, Chaoyangmen Patentee after: CHINA PETROLEUM & CHEMICAL Corp. Patentee after: Sinopec (Dalian) Petrochemical Research Institute Co.,Ltd. Address before: 100728 No. 22 North Main Street, Chaoyang District, Beijing, Chaoyangmen Patentee before: CHINA PETROLEUM & CHEMICAL Corp. Patentee before: DALIAN RESEARCH INSTITUTE OF PETROLEUM AND PETROCHEMICALS, SINOPEC Corp. |
|
TR01 | Transfer of patent right |