CN105713856B - It is a kind of to improve the biodegradable method of sulfamethoxazole using vitamin - Google Patents

It is a kind of to improve the biodegradable method of sulfamethoxazole using vitamin Download PDF

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Publication number
CN105713856B
CN105713856B CN201610107161.XA CN201610107161A CN105713856B CN 105713856 B CN105713856 B CN 105713856B CN 201610107161 A CN201610107161 A CN 201610107161A CN 105713856 B CN105713856 B CN 105713856B
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vitamin
sulfamethoxazole
seed
degradation
degradation solution
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CN105713856A (en
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程景胜
张艺碧
元英进
周娇
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Tianjin University
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Tianjin University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/38—Organic compounds containing nitrogen
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/40—Organic compounds containing sulfur
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
    • C02F2103/343—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the pharmaceutical industry, e.g. containing antibiotics
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
    • C02F2103/36—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the manufacture of organic compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00—Use of specific compounds during water treatment
    • C02F2305/06—Nutrients for stimulating the growth of microorganisms

Abstract

The biodegradable method of sulfamethoxazole is improved using vitamin the invention discloses a kind of, include the following steps: (1) seed culture: the Bacillus foecalis alkaligenes (Alcaligenes faecalis) that deposit number is CGMCC NO.1.767 being cultivated in seed culture medium, obtains first order seed；First order seed is cultivated in seed culture medium, obtains secondary seed；(2) degradation solution is prepared: sulfamethoxazole production waste discharge 80mL being added in the basic inorganic salt culture medium that 20mL contains vitamin and is made into degradation solution；(3) fermentative degradation: in the Bacillus foecalis alkaligenes secondary seed access 100mL degradation solution that step (1) is obtained, fermentation.The present invention is to add vitamin during Bacillus foecalis alkaligenes degrades sulfamethoxazole, compared to the basic inorganic salt culture medium for being not added with vitamin, remarkably promotes the degradation of SMX, the concentration of SMX is made to drop to lower concentration.

Description

It is a kind of to improve the biodegradable method of sulfamethoxazole using vitamin
Technical field
The invention belongs to biotechnologys and field of environment pollution control, relate to the use of vitamin and improve sulfamethoxazole biology The method of degradation.
Background technique
Representative drug of the sulfamethoxazole (SMX) as sulfamido, is widely used in animal husbandry and aquaculture.It is domestic Outer existing research, which is reported in rivers and lakes, underground water, sewage treatment plant and hospital wastewater, detects ng/L~mg/L concentration SMX.Artificial synthesized SMX is not degradable in the natural environment, and long-term existence causes microorganism to generate drug resistance in environment With the diffusion of resistant gene, human health is seriously threatened.Biodegrade at present is the master for removing sulfamethoxazole in water body environment Want method, using the natural mixed flora in the activated sludge of sewage treatment plant or the sedimentary clay of rivers and lakes, they using SMX as Degrade SMX while carbon source and energy substance growth.Sludge biodegradation sulfa drugs there are the problem of have it is processed The journey period is long, and degradation efficiency is low and performance is unstable etc..
Summary of the invention
It is a kind of biological using vitamin raising sulfamethoxazole the purpose of the present invention is overcoming the deficiencies of the prior art and provide The method of degradation.
Technical solution of the present invention is summarized as follows:
It is a kind of to improve the biodegradable method of sulfamethoxazole using vitamin, include the following steps:
(1) seed culture: the Bacillus foecalis alkaligenes for being CGMCC NO.1.767 by deposit number (Alcaligenesfaecalis) it is cultivated in seed culture medium, obtains first order seed；First order seed is trained in seed culture medium It supports, obtains secondary seed；
(2) degradation solution is prepared: sulfamethoxazole production waste discharge 80mL is added to the basis that 20mL contains vitamin Degradation solution is made into minimal medium, the basic inorganic salt culture medium containing vitamin is counted with g/L to be formed: FeSO4· 7H2O 0.1,MgSO4·7H2O 1.5,(NH4)2SO4 5.0,Na2EDTA·2H2O 0.095,CaCl2·2H2O 0.075, Na2HPO4 40,KH2PO430, glucose 10, sodium acetate 10, vitamin 0.001~0.1, surplus is water；
(3) fermentative degradation: the Bacillus foecalis alkaligenes secondary seed that step (1) is obtained is with initial OD540For 0.1~0.3 access It is 200~240rpm, fermentation 40~50 under the conditions of temperature is 28~32 DEG C in revolving speed in the 100mL degradation solution that step (2) obtains Hour.
The vitamin is vitamin C, vitamin B2, vitamin B6Or vitamin B12。
The invention has the advantages that
The present invention is to add vitamin during Bacillus foecalis alkaligenes degrades sulfamethoxazole, compared to being not added with vitamin Basic inorganic salt culture medium, remarkably promote the degradation of SMX, the concentration of SMX made to drop to lower concentration.
Specific embodiment
The present invention is further described combined with specific embodiments below:
Bacillus foecalis alkaligenes (Alcaligenesfaecalis) of the present invention is bought in Chinese microorganism strain preservation Administration committee's common micro-organisms center, deposit number: CGMCC NO.1.767.
Embodiment 1
The preparation of seed culture medium
Seed culture based component is in terms of g/L: beef extract 3, NaCl 5, peptone 10, and 7.0,121 DEG C of steam of pH go out Bacterium 20min.
Embodiment 2
It is a kind of to improve the biodegradable method of sulfamethoxazole using vitamin, include the following steps:
(1) seed culture: the Bacillus foecalis alkaligenes (Alcaligenes for being CGMCC NO.1.767 by deposit number Faecalis it) is cultivated in the seed culture medium that embodiment 1 is prepared, obtains first order seed；First order seed is prepared in embodiment 1 It is cultivated in seed culture medium, obtains secondary seed；
(2) degradation solution is prepared: sulfamethoxazole production waste discharge 80mL is added to the basis that 20mL contains vitamin Degradation solution (concentration of sulfamethoxazole is 50mg/L in degradation solution), the base containing vitamin are made into minimal medium Plinth minimal medium is counted with g/L to be formed: FeSO4·7H2O 0.1,MgSO4·7H2O 1.5,(NH4)2SO4 5.0, Na2EDTA·2H2O 0.095,CaCl2·2H2O 0.075,Na2HPO4 40,KH2PO430, glucose 10, sodium acetate 10, dimension Raw element C 0.001, surplus is water；
(3) fermentative degradation: the Bacillus foecalis alkaligenes secondary seed that step (1) is obtained is with initial OD540For 0.2 access step It (2) is 220rpm in revolving speed, temperature is fermented 45 hours under the conditions of being 30 DEG C in the 100mL degradation solution obtained.
Detection, SMX concentration are 4.89mg/L.
Embodiment 3
It is a kind of to improve the biodegradable method of sulfamethoxazole using vitamin, include the following steps:
(1) seed culture, with 2 step of embodiment (1)；
(2) degradation solution is prepared: sulfamethoxazole production waste discharge 80mL is added to the basis that 20mL contains vitamin Degradation solution (concentration of sulfamethoxazole is 50mg/L in degradation solution), the base containing vitamin are made into minimal medium Plinth minimal medium is counted with g/L to be formed: FeSO4·7H2O 0.1,MgSO4·7H2O 1.5,(NH4)2SO4 5.0, Na2EDTA·2H2O 0.095,CaCl2·2H2O 0.075,Na2HPO4 40,KH2PO430, glucose 10, sodium acetate 10, dimension Raw element C 0.1, surplus is water；
(3) fermentative degradation: the Bacillus foecalis alkaligenes secondary seed that step (1) is obtained is with initial OD540For 0.1 access step It (2) is 200rpm in revolving speed, temperature is fermented 50 hours under the conditions of being 28 DEG C in the 100mL degradation solution obtained.
Detection, SMX concentration are 2.70mg/L.
Embodiment 4
It is a kind of to improve the biodegradable method of sulfamethoxazole using vitamin, include the following steps:
(1) seed culture, with 2 step of embodiment (1)；
(2) degradation solution is prepared: sulfamethoxazole production waste discharge 80mL is added to the basis that 20mL contains vitamin Degradation solution (concentration of sulfamethoxazole is 50mg/L in degradation solution), the base containing vitamin are made into minimal medium Plinth minimal medium is counted with g/L to be formed: FeSO4·7H2O 0.1,MgSO4·7H2O 1.5,(NH4)2SO4 5.0, Na2EDTA·2H2O 0.095,CaCl2·2H2O 0.075,Na2HPO4 40,KH2PO430, glucose 10, sodium acetate 10, dimension Raw element C 0.01, surplus is water；
(3) fermentative degradation: the Bacillus foecalis alkaligenes secondary seed that step (1) is obtained is with initial OD540For 0.3 access step It (2) is 240rpm in revolving speed, temperature is fermented 40 hours under the conditions of being 32 DEG C in the 100mL degradation solution obtained.
Detection, SMX concentration are 4.38mg/L.
Embodiment 5
It is a kind of to improve the biodegradable method of sulfamethoxazole using vitamin, include the following steps:
(1) seed culture, with 2 step of embodiment (1)
(2) degradation solution is prepared: sulfamethoxazole production waste discharge 80mL is added to the basis that 20mL contains vitamin Degradation solution (concentration of sulfamethoxazole is 50mg/L in degradation solution), the base containing vitamin are made into minimal medium Plinth minimal medium is counted with g/L to be formed: FeSO4·7H2O 0.1,MgSO4·7H2O 1.5,(NH4)2SO4 5.0, Na2EDTA·2H2O 0.095,CaCl2·2H2O 0.075,Na2HPO4 40,KH2PO430, glucose 10, sodium acetate 10, dimension Raw element B20.001, surplus is water；
(3) fermentative degradation: the Bacillus foecalis alkaligenes secondary seed that step (1) is obtained is with initial OD540For 0.2 access step It (2) is 220rpm in revolving speed, temperature is fermented 45 hours under the conditions of being 30 DEG C in the 100mL degradation solution obtained.
Detection, SMX concentration are 5.23mg/L.
Embodiment 6
It is a kind of to improve the biodegradable method of sulfamethoxazole using vitamin, include the following steps:
(1) seed culture, with 2 step of embodiment (1)
(2) degradation solution is prepared: sulfamethoxazole production waste discharge 80mL is added to the basis that 20mL contains vitamin Degradation solution (concentration of sulfamethoxazole is 50mg/L in degradation solution), the base containing vitamin are made into minimal medium Plinth minimal medium is counted with g/L to be formed: FeSO4·7H2O 0.1,MgSO4·7H2O 1.5,(NH4)2SO4 5.0, Na2EDTA·2H2O 0.095,CaCl2·2H2O 0.075,Na2HPO4 40,KH2PO430, glucose 10, sodium acetate 10, dimension Raw element B20.1, surplus is water；
(3) fermentative degradation: the Bacillus foecalis alkaligenes secondary seed that step (1) is obtained is with initial OD540For 0.1 access step It (2) is 200rpm in revolving speed, temperature is fermented 50 hours under the conditions of being 28 DEG C in the 100mL degradation solution obtained.
Detection, SMX concentration are 5.93mg/L.
Embodiment 7
It is a kind of to improve the biodegradable method of sulfamethoxazole using vitamin, include the following steps:
(1) seed culture, with 2 step of embodiment (1)；
(2) degradation solution is prepared: sulfamethoxazole production waste discharge 80mL is added to the basis that 20mL contains vitamin Degradation solution (concentration of sulfamethoxazole is 50mg/L in degradation solution), the base containing vitamin are made into minimal medium Plinth minimal medium is counted with g/L to be formed: FeSO4·7H2O 0.1,MgSO4·7H2O 1.5,(NH4)2SO4 5.0, Na2EDTA·2H2O 0.095,CaCl2·2H2O 0.075,Na2HPO4 40,KH2PO430, glucose 10, sodium acetate 10, dimension Raw element B20.01, surplus is water；
(3) fermentative degradation: the Bacillus foecalis alkaligenes secondary seed that step (1) is obtained is with initial OD540For 0.3 access step It (2) is 240rpm in revolving speed, temperature is fermented 40 hours under the conditions of being 32 DEG C in the 100mL degradation solution obtained.
Detection, SMX concentration are 3.62mg/L.
Embodiment 8
It is a kind of to improve the biodegradable method of sulfamethoxazole using vitamin, include the following steps:
(1) seed culture, with 2 step of embodiment (1)；
(2) degradation solution is prepared: sulfamethoxazole production waste discharge 80mL is added to the basis that 20mL contains vitamin Degradation solution (concentration of sulfamethoxazole is 50mg/L in degradation solution), the base containing vitamin are made into minimal medium Plinth minimal medium is counted with g/L to be formed: FeSO4·7H2O 0.1,MgSO4·7H2O 1.5,(NH4)2SO4 5.0, Na2EDTA·2H2O 0.095,CaCl2·2H2O 0.075,Na2HPO4 40,KH2PO430, glucose 10, sodium acetate 10, dimension Raw element B60.001, surplus is water；
(3) fermentative degradation: the Bacillus foecalis alkaligenes secondary seed that step (1) is obtained is with initial OD540For 0.2 access step It (2) is 220rpm in revolving speed, temperature is fermented 45 hours under the conditions of being 30 DEG C in the 100mL degradation solution obtained.
Detection, SMX concentration are 3.50mg/L.
Embodiment 9
It is a kind of to improve the biodegradable method of sulfamethoxazole using vitamin, include the following steps:
(1) seed culture, with 2 step of embodiment (1)；
(2) degradation solution is prepared: sulfamethoxazole production waste discharge 80mL is added to the basis that 20mL contains vitamin Degradation solution (concentration of sulfamethoxazole is 50mg/L in degradation solution), the base containing vitamin are made into minimal medium Plinth minimal medium is counted with g/L to be formed: FeSO4·7H2O 0.1,MgSO4·7H2O 1.5,(NH4)2SO4 5.0, Na2EDTA·2H2O 0.095,CaCl2·2H2O 0.075,Na2HPO4 40,KH2PO430, glucose 10, sodium acetate 10, dimension Raw element B60.1, surplus is water；
(3) fermentative degradation: the Bacillus foecalis alkaligenes secondary seed that step (1) is obtained is with initial OD540For 0.1 access step It (2) is 200rpm in revolving speed, temperature is fermented 50 hours under the conditions of being 28 DEG C in the 100mL degradation solution obtained.
Detection, SMX concentration are 2.78mg/L.
Embodiment 10
It is a kind of to improve the biodegradable method of sulfamethoxazole using vitamin, include the following steps:
(1) seed culture, with 2 step of embodiment (1)；
(2) degradation solution is prepared: sulfamethoxazole production waste discharge 80mL is added to the basis that 20mL contains vitamin Degradation solution (concentration of sulfamethoxazole is 50mg/L in degradation solution), the base containing vitamin are made into minimal medium Plinth minimal medium is counted with g/L to be formed: FeSO4·7H2O 0.1,MgSO4·7H2O 1.5,(NH4)2SO4 5.0, Na2EDTA·2H2O 0.095,CaCl2·2H2O 0.075,Na2HPO4 40,KH2PO430, glucose 10, sodium acetate 10, dimension Raw element B60.01, surplus is water；
(3) fermentative degradation: the Bacillus foecalis alkaligenes secondary seed that step (1) is obtained is with initial OD540For 0.3 access step It (2) is 240rpm in revolving speed, temperature is fermented 40 hours under the conditions of being 32 DEG C in the 100mL degradation solution obtained.
Detection, SMX concentration are 3.12mg/L.
Embodiment 11
It is a kind of to improve the biodegradable method of sulfamethoxazole using vitamin, include the following steps:
(1) seed culture, with 2 step of embodiment (1)；
(2) degradation solution is prepared: sulfamethoxazole production waste discharge 80mL is added to the basis that 20mL contains vitamin Degradation solution (concentration of sulfamethoxazole is 50mg/L in degradation solution), the base containing vitamin are made into minimal medium Plinth minimal medium is counted with g/L to be formed: FeSO4·7H2O 0.1,MgSO4·7H2O 1.5,(NH4)2SO4 5.0, Na2EDTA·2H2O 0.095,CaCl2·2H2O 0.075,Na2HPO4 40,KH2PO430, glucose 10, sodium acetate 10, dimension Raw element B120.001, surplus is water；
(3) fermentative degradation: the Bacillus foecalis alkaligenes secondary seed that step (1) is obtained is with initial OD540For 0.2 access step It (2) is 220rpm in revolving speed, temperature is fermented 45 hours under the conditions of being 30 DEG C in the 100mL degradation solution obtained.
Detection, SMX concentration are 4.00mg/L.
Embodiment 12
It is a kind of to improve the biodegradable method of sulfamethoxazole using vitamin, include the following steps:
(1) seed culture, with 2 step of embodiment (1)；
(2) degradation solution is prepared: sulfamethoxazole production waste discharge 80mL is added to the basis that 20mL contains vitamin Degradation solution (concentration of sulfamethoxazole is 50mg/L in degradation solution), the base containing vitamin are made into minimal medium Plinth minimal medium is counted with g/L to be formed: FeSO4·7H2O 0.1,MgSO4·7H2O 1.5,(NH4)2SO4 5.0, Na2EDTA·2H2O 0.095,CaCl2·2H2O 0.075,Na2HPO4 40,KH2PO430, glucose 10, sodium acetate 10, dimension Raw element B120.1, surplus is water；
(3) fermentative degradation: the Bacillus foecalis alkaligenes secondary seed that step (1) is obtained is with initial OD540For 0.1 access step It (2) is 200rpm in revolving speed, temperature is fermented 50 hours under the conditions of being 28 DEG C in the 100mL degradation solution obtained.
Detection, SMX concentration are 3.98mg/L.
Embodiment 13
It is a kind of to improve the biodegradable method of sulfamethoxazole using vitamin, include the following steps:
(1) seed culture, with 2 step of embodiment (1)；
(2) degradation solution is prepared: sulfamethoxazole production waste discharge 80mL is added to the basis that 20mL contains vitamin Degradation solution (concentration of sulfamethoxazole is 50mg/L in degradation solution), the base containing vitamin are made into minimal medium Plinth minimal medium is counted with g/L to be formed: FeSO4·7H2O 0.1,MgSO4·7H2O 1.5,(NH4)2SO4 5.0, Na2EDTA·2H2O 0.095,CaCl2·2H2O 0.075,Na2HPO4 40,KH2PO430, glucose 10, sodium acetate 10, dimension Raw element B120.01, surplus is water；
(3) fermentative degradation: the Bacillus foecalis alkaligenes secondary seed that step (1) is obtained is with initial OD540For 0.3 access step It (2) is 240rpm in revolving speed, temperature is fermented 40 hours under the conditions of being 32 DEG C in the 100mL degradation solution obtained.
Detection, SMX concentration are 3.83mg/L.
Embodiment 14
Comparative example includes the following steps:
(1) seed culture, with 2 step of embodiment (1)；
(2) degradation solution is prepared: sulfamethoxazole production waste discharge 80mL is added to 20mL basic inorganic salt culture medium In be made into degradation solution (in degradation solution the concentration of sulfamethoxazole be 50mg/L), basic inorganic salt culture medium group in terms of g/L At: FeSO4·7H2O 0.1,MgSO4·7H2O 1.5,(NH4)2SO4 5.0,Na2EDTA·2H2O 0.095,CaCl2·2H2O 0.075,Na2HPO4 40,KH2PO430, glucose 10, sodium acetate 10, surplus is water；
(3) fermentative degradation: the Bacillus foecalis alkaligenes secondary seed that step (1) is obtained is with initial OD540For 0.2 access step It (2) is 220rpm in revolving speed, temperature is fermented 45 hours under the conditions of being 30 DEG C in the 100mL degradation solution obtained.
Detection, SMX concentration are 20.41mg/L.
The detection method of the concentration of SMX is efficient liquid phase.
Chromatographic column used in high performance liquid chromatography is C18 column (250 × 4.6mm, 5 μm), and sample volume is 10 μ L, and mobile phase is Acetonitrile: 0.1% aqueous formic acid=40:60, flow velocity 1mL.min-1, Detection wavelength 265nm, column temperature maintains 30 DEG C.

Claims (1)

1. a kind of improve the biodegradable method of sulfamethoxazole using vitamin, feature includes the following steps:
(1) seed culture: the Bacillus foecalis alkaligenes (Alcaligenes faecalis) for being CGMCC NO.1.767 by deposit number It is cultivated in seed culture medium, obtains first order seed；First order seed is cultivated in seed culture medium, obtains secondary seed；
(2) degradation solution is prepared: sulfamethoxazole production waste discharge 80mL is added to the basic inorganic that 20mL contains vitamin Degradation solution is made into salt culture medium, the basic inorganic salt culture medium containing vitamin is counted with g/L to be formed: FeSO4·7H2O 0.1,MgSO4·7H2O 1.5,(NH4)2SO4 5.0,Na2EDTA·2H2O 0.095,CaCl2·2H2O 0.075,Na2HPO4 40,KH2PO430, glucose 10, sodium acetate 10, vitamin 0.001~0.1, surplus is water；
(3) fermentative degradation: the Bacillus foecalis alkaligenes secondary seed that step (1) is obtained is with initial OD540For 0.1~0.3 access step It (2) is 200~240rpm in revolving speed, fermentation 40~50 is small under the conditions of temperature is 28~32 DEG C in the 100mL degradation solution obtained When；The vitamin is vitamin C, vitamin B2, vitamin B6Or vitamin B12。
CN201610107161.XA 2016-02-26 2016-02-26 It is a kind of to improve the biodegradable method of sulfamethoxazole using vitamin Expired - Fee Related CN105713856B (en)

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CN106380001A (en) * 2016-08-31 2017-02-08 天津大学 Method for degrading sulfanilamide compounds in water environment by use of mixed bacteria
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