CN103896393A - Method for cultivating high strength aerobic granular sludge - Google Patents
Method for cultivating high strength aerobic granular sludge Download PDFInfo
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- CN103896393A CN103896393A CN201410021855.2A CN201410021855A CN103896393A CN 103896393 A CN103896393 A CN 103896393A CN 201410021855 A CN201410021855 A CN 201410021855A CN 103896393 A CN103896393 A CN 103896393A
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Abstract
The invention belongs to the technical field of environmental protection and in particular relates to a method for cultivating high strength aerobic granular sludge. The method for cultivating high strength aerobic granular sludge comprises that refluxed activated sludge of a municipal sewage plant is taken as seed sludge, sodium acetate and sodium propionate are taken as a combined carbon source, and quick cultivation of high strength aerobic granular sludge is carried out in a sequencing batch intermittent technology at the temperature of 25 minus or plus 1 DEG C; after 20 days, mature aerobic granular sludge is formed, and organic matter removal capacity above 90% is realized; meanwhile, the aerobic granular sludge can maintain a stable structure for a longer time and is not broken within 15 minutes under the ultrasonic conditions of 20KHz and 65W.
Description
Technical field
The invention belongs to environmental technology field, be specifically related to a kind of cultural method of aerobic particle mud.
Background technology
Compared with the aerobic activated sludge process using with current most of municipal wastewater treatment plant second-stage treatment, the typical feature of aerobic particle mud is can carry out self-immobilization without any need for carrier.First this show as its fine and close three-dimensional arrangement, not only can guarantee the biomass that remains higher in biological treatment, can also provide provide protection for inner microorganism by this three-dimensional arrangement, therefore can bear unexpected environment change; Next has superior settling property, and this makes not need in biological treatment the later separation program of existing aerobic particle mud in processing, and has saved time consumption and the area of reaction process.Its required land area only needs 20% of traditional aerobic activated sludge process, more has economic benefit in the application aspect with the sewage treatment process in population high-density city.
For bio-reactor water technology, in ensureing art breading usefulness, there is good separating effect and there is very important using value, therefore, technical superiority and the market potential of aerobic grain in sewage treatment project is very obvious.But because the structural phenomenon of disintegrating appears in granule sludge under prolonged operation condition, cause the loss of biomass, and then can affect the operation usefulness of technique, therefore, ensure grain pattern in technique long-time running, keep efficient stable be aerobic grain technology can engineering the important prerequisite basis of application.In recent years, scholar both domestic and external had carried out a large amount of test works to the reason that causes aerobic grain unstability, mainly contained following several respects reason: the hypertrophy of (1) filamentous organisms; (2) granular core anaerobic hydrolysis; (3) bacterial strain afunction etc.
Based on the above-mentioned research that causes aerobic particle mud cause of destabilization about existing, generally in the time cultivating aerobic particle mud, all adopt single carbon source to cultivate, such as glucose, acetic acid, phenol, starch, alcohol, sucrose and other synthetic wastewaters are all used to cultivate aerobic particle mud.For population domestication, single carbon source easily causes the simplification of population metabolism in mud, and the aerobic particle mud compound population with special three-dimensional arrangement that to be a cooperation on multiple populations maintain jointly.Therefore, the metabolic characteristics that the present invention is based on carbon source proposes a kind of acetic acid and propionic acid carry out aerobic particle mud cultivation jointly method as carbon source, strengthen the diversity of population by the complicated feature that increases carbon metabolism, improve the intensity of aerobic particle mud, adapt to the needs of wastewater treatment.
Summary of the invention
The object of the present invention is to provide a kind of can carrying out under normal temperature condition, fast, technique is simply cultivated the method for aerobic particle mud, and gained aerobic particle mud is high to the clearance of COD, rock steady structure.
The method of cultivation aerobic particle mud provided by the invention, under 25 ± 1 DEG C of conditions, jointly as carbon source, realizes the fast culture of the aerobic particle mud with high strength feature using acetic acid and propionic acid by sequence batch (batch technology (being SBR).
The concrete operations of the inventive method are as follows:
(1) inoculation of activated-sludge
Active sludge is inoculated in sbr reactor device, and the reactor of sbr reactor device is cylindrical, and internal diameter is 6cm, high 180cm, and effective working volume is about 2.3L, the 1.6 ± 0.3L of at every turn intaking, the outside circulating water heat insulation that adopts of reactor, temperature is controlled at 25 ± 1 DEG C;
(2) water inlet
Between aerobic particle mud incubation period, the synthetic sewage that employing sodium acetate and Sodium Propionate are carbon source is as water inlet, and the component of substantially intaking comprises: NH
4cl 0.2 ± 0.1g/L, KH
2pO
40.66 ± 0.1g/L, K
2hPO
41.22 ± 0.3g/L, CaCl
20.03 ± 0.01 g/L, MgSO
47H
2o 0.025 ± 0.01g/L.FeSO
45H
2o 0.02 ± 0.01g/L, NaHCO
30.013 ± 0.003g/L; Peptone 0.4 ± 0.1g/L, yeast powder 0.25 ± 0.1g/L; Influent COD is provided by sodium acetate and Sodium Propionate, wherein the ratio that adds of sodium acetate and Sodium Propionate is scaled within the scope of 2:1 ~ 4:1 according to COD, initial influent COD is 1000 ± 100 mg/L, regulates influent COD value subsequently according to the different operation phase, arranges concretely as described in Table 1; Finally, be 7.2 ± 0.1 by the synthetic sewage pH regulator configuring;
(3) operation
Seed sludge concentration is 6000 ± 500mg/L, and aeration rate is 3 ~ 5 L/min; Sbr reactor device moves 6 cycles every day, each cycles 4 h, and one-period comprises: fill phase 3 ± 1 min, aeration phase and subsidence stage be totally 227 ± 3 min, idle stages 5 ± 1 min, bleeding stage 5 ± 1 min;
Between whole granule sludge incubation period, be 20-30 days, be divided into six process sections (in table 1), wherein, the subsidence stage time of mud was adjusted according to the different operation phase, and the scheme of specifically adjusting is as described in Table 1; Meanwhile, based on the continuous shortening of settling time, aeration time correspondingly extends gradually, to ensure that aeration phase and subsidence stage amount to approximately 227 ± 3 min.
Table 1 SBR regulate and control operation mode
Result of the present invention shows, under normal temperature condition, jointly as carbon source, can obtain the aerobic particle mud with efficient processing efficiency and high strength characteristics using acetic acid and propionic acid by sequence batch (batch technology, this aerobic particle mud can reach more than 90% the average removal rate of COD.Meanwhile, under the ultrasound condition of 20 KHz, 65W, can to maintain 15min not broken for this particle, maintains rock steady structure.
Brief description of the drawings
Fig. 1 is ripe aerobic particle mud morphological specificity.
Fig. 2 is the removal effect of COD.
Fig. 3 is the strength analysis of aerobic particle mud in example 1.
Fig. 4 obtains to stablize ripe aerobic particle mud diagram in example 2 the 20th day.
Fig. 5 is the strength analysis of aerobic particle mud in example 2.
Embodiment
Below by embodiment, further describe the present invention.
embodiment 1, the cultivation of aerobic particle mud
The cotton-shaped returned sluge of fetching from Shanghai sewage work after precipitation, is got rid of supernatant liquor under room temperature, and measuring concentrated activated sludge concentration is 19714 mg/L.At ambient temperature, in the sbr reactor device that inoculation 700 mL mud to useful volume is 2.3L.Each cycle of operation was divided into into water-aeration-precipitation-draining-idle 5 stages.During water inlet, start intake pump and will synthesize sewage injecting reactor, reach after reactor useful volume, stop into water, the time is 3 min; Aeration phase and subsidence stage be totally 227 min, and aeration rate 5 L/min, between granule sludge incubation period, are divided into six stages by technique, and the settling time of mud was adjusted according to the different operation phase, and the scheme of specifically adjusting is as described in Table 1; Between drainage period, start wet-pit and draw the supernatant liquor after precipitation, the time is 5min; Enter subsequently the idle stage, wait for the operation of next cycle.Between aerobic grain incubation period, mud particle diameter increases gradually, within the 20th day, obtains and stablizes ripe aerobic particle mud, as shown in Figure 1.This aerobic particle mud culture process to the removal effect of COD as shown in Figure 2.When technique self-operating starts, the clearance of COD can reach 80% left and right.Along with the continuous service of technique, COD clearance can reach 90% at 5d, and at whole aerobic particle mud culture process run duration, COD clearance all can maintain more than 90% subsequently.Secondly the ripe aerobic particle mud that the mode that, adopts ultrasonication forms after to 20d carries out intensity evaluation.In autoreaction device, get at random a certain amount of aerobic particle mud and be placed in the beaker that pure water is housed, be 20 KHz in frequency, power is under 65 W conditions, aerobic particle mud is carried out to supersound process, interval certain hour is measured the absorbance (be OD600 value) of the aqueous solution under 600nm subsequently, and result as shown in Figure 3.OD600 value in water increases gradually with the prolongation of ultrasonic time, until after 15min, OD600 value just remains stable after reaching 0.08 left and right, show that this aerobic particle mud can be completely broken after 15min, confirm that this granule strength is high, aerobic particle mud Stability Analysis of Structures.
embodiment 2, the cultivation of aerobic particle mud
The cotton-shaped returned sluge of fetching from Shanghai sewage work after precipitation, is got rid of supernatant liquor under room temperature, and measuring concentrated activated sludge concentration is 19714 mg/L.At ambient temperature, in the sbr reactor device that inoculation 700 mL mud to useful volume is 2.3L.Each cycle of operation was divided into into water-aeration-precipitation-draining-idle 5 stages.During water inlet, start intake pump and will synthesize sewage injecting reactor, reach after reactor useful volume, stop into water, the time is 3 min; Aeration phase and subsidence stage be totally 227 min, and aeration rate 3 L/min, between granule sludge incubation period, are divided into six stages by technique, and the settling time of mud was adjusted according to the different operation phase, and the scheme of specifically adjusting is as described in Table 1; Between drainage period, start wet-pit and draw the supernatant liquor after precipitation, the time is 5min; Enter subsequently the idle stage, wait for the operation of next cycle.Between aerobic grain incubation period, mud particle diameter increases gradually, within the 20th day, obtains and stablizes ripe aerobic particle mud, as shown in Figure 4.The ripe aerobic particle mud forming after to 20d by the mode of ultrasonication carries out intensity evaluation.In autoreaction device, get at random a certain amount of aerobic particle mud and be placed in the beaker that pure water is housed, be 20 KHz in frequency, power is under 65 W conditions, aerobic particle mud is carried out to supersound process, interval certain hour is measured the absorbance (be OD600 value) of the aqueous solution under 600nm subsequently, and result as shown in Figure 5.OD600 value in water increases gradually with the prolongation of ultrasonic time, until after 20min, OD600 value just remains stable after reaching 0.26 left and right, show that this aerobic particle mud can be completely broken after 20min, confirm that this granule strength is high, aerobic particle mud Stability Analysis of Structures.
Claims (2)
1. a cultural method for high strength aerobic granular sludge, is characterized in that under 25 ± 1 DEG C of conditions, jointly as carbon source, carries out the fast culture of aerobic particle mud using acetic acid and propionic acid by sequence batch (batch technology (SBR).
2. cultural method according to claim 1, is characterized in that specific operation process is as follows:
(1) inoculation of activated-sludge
Active sludge is inoculated in sbr reactor device, and the reactor of sbr reactor device is cylindrical, and internal diameter is 6cm, high 180cm, and effective working volume is about 2.3L, the 1.6 ± 0.3L of at every turn intaking, the outside circulating water heat insulation that adopts of reactor, temperature is controlled at 25 ± 1 DEG C;
(2) water inlet
Between aerobic particle mud incubation period, the synthetic sewage that employing sodium acetate and Sodium Propionate are carbon source is as water inlet, and the component of substantially intaking comprises: NH
4cl 0.2 ± 0.1g/L, KH
2pO
40.66 ± 0.1g/L, K
2hPO
41.22 ± 0.3g/L, CaCl
20.03 ± 0.01 g/L, MgSO
47H
2o 0.025 ± 0.01g/L; FeSO
45H
2o 0.02 ± 0.01g/L, NaHCO
30.013 ± 0.003g/L; Peptone 0.4 ± 0.1g/L, yeast powder 0.25 ± 0.1g/L; Influent COD is provided by sodium acetate and Sodium Propionate, wherein the ratio that adds of sodium acetate and Sodium Propionate is scaled within the scope of 2:1 ~ 4:1 according to COD, initial influent COD is 1000 ± 100 mg/L, regulates influent COD value subsequently according to the different operation phase, arranges concretely as described in Table 1; Finally, be 7.2 ± 0.1 by the synthetic sewage pH regulator configuring;
(3) operation
Seed sludge concentration is 6000 ± 500mg/L, and aeration rate is 3 ~ 5 L/min; Sbr reactor device moves 6 cycles every day, each cycles 4 h, and one-period comprises: fill phase 3 ± 1min, aeration phase and subsidence stage be totally 227 ± 3 min, idle stages 5 ± 1 min, bleeding stage 5 ± 1 min;
Between whole granule sludge incubation period, be 20-30 days, be divided into six process sections, wherein, in each cycle of operation, the subsidence stage time of mud is adjusted according to different operation process sections, and the scheme of specifically adjusting is as described in Table 1; Meanwhile, based on the continuous shortening of settling time, aeration time correspondingly extends to ensure that aeration phase and subsidence stage amount to 227 ± 3 min gradually;
SBR regulate and control operation mode:
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Cited By (6)
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| CN104649405A (en) * | 2015-03-20 | 2015-05-27 | 中蓝连海设计研究院 | Cultivating method for activated sludge for biochemically treating high-temperature salt-containing wastewater |
| CN105645574A (en) * | 2016-01-05 | 2016-06-08 | 井冈山大学 | Method for promoting excess sludge to cultivate aerobic granular sludge by adding soapnut saponin |
| CN107531525A (en) * | 2015-03-31 | 2018-01-02 | 奥加诺株式会社 | The forming method of aerobic grain, the forming apparatus of aerobic grain, drainage processing method and drain treatment apparatus |
| CN108217922A (en) * | 2016-12-22 | 2018-06-29 | 光大水务(济南)有限公司 | Municipal wastewater processing method |
| CN109095601A (en) * | 2018-10-25 | 2018-12-28 | 江西理工大学 | Aerobic granular sludge reactor operation stability based on size controlling maintains method |
| CN113998778A (en) * | 2021-10-28 | 2022-02-01 | 华东理工大学 | Aerobic granular sludge rapid culture method for treating high-salinity wastewater |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104649405A (en) * | 2015-03-20 | 2015-05-27 | 中蓝连海设计研究院 | Cultivating method for activated sludge for biochemically treating high-temperature salt-containing wastewater |
| CN107531525A (en) * | 2015-03-31 | 2018-01-02 | 奥加诺株式会社 | The forming method of aerobic grain, the forming apparatus of aerobic grain, drainage processing method and drain treatment apparatus |
| CN105645574A (en) * | 2016-01-05 | 2016-06-08 | 井冈山大学 | Method for promoting excess sludge to cultivate aerobic granular sludge by adding soapnut saponin |
| CN105645574B (en) * | 2016-01-05 | 2018-01-19 | 井冈山大学 | Add the method that sapindoside promotes excess sludge culture aerobic particle mud |
| CN108217922A (en) * | 2016-12-22 | 2018-06-29 | 光大水务(济南)有限公司 | Municipal wastewater processing method |
| CN108217922B (en) * | 2016-12-22 | 2020-05-19 | 光大水务(济南)有限公司 | Municipal sewage treatment method |
| CN109095601A (en) * | 2018-10-25 | 2018-12-28 | 江西理工大学 | Aerobic granular sludge reactor operation stability based on size controlling maintains method |
| CN109095601B (en) * | 2018-10-25 | 2021-07-06 | 江西理工大学 | Method for maintaining operation stability of aerobic granular sludge reactor based on particle size control |
| CN113998778A (en) * | 2021-10-28 | 2022-02-01 | 华东理工大学 | Aerobic granular sludge rapid culture method for treating high-salinity wastewater |
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Application publication date: 20140702 |