CN105695488A - New application of Arabidopsis Thaliana gene At1G21640 in plant drought resistance - Google Patents

New application of Arabidopsis Thaliana gene At1G21640 in plant drought resistance Download PDF

Info

Publication number
CN105695488A
CN105695488A CN201610246041.8A CN201610246041A CN105695488A CN 105695488 A CN105695488 A CN 105695488A CN 201610246041 A CN201610246041 A CN 201610246041A CN 105695488 A CN105695488 A CN 105695488A
Authority
CN
China
Prior art keywords
gene
nadk2
at1g21640
plant
drought
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610246041.8A
Other languages
Chinese (zh)
Inventor
郝福顺
孙立荣
李亚平
郝杨
苗琛
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Henan University
Original Assignee
Henan University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Henan University filed Critical Henan University
Priority to CN201610246041.8A priority Critical patent/CN105695488A/en
Publication of CN105695488A publication Critical patent/CN105695488A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01023—NAD+ kinase (2.7.1.23)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/13—Abiotic stress
    • Y02A40/132—Plants tolerant to drought

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention belongs to the technical field of genetic engineering, and particularly relates to application for patent of new application of Arabidopsis Thaliana gene At1G21640 in plant drought resistance. According to tests, sensitivity of mutant plants to drought stress is increased after deletion of the gene At1G21640 ; tolerance of mutant plants to drought stress is increased after over-expression of the gene At1G21640; accordingly, the Arabidopsis Thaliana gene At1G21640 is related to plant drought resistance function. According to a series of physiological researches on plants with afunctional mutants namely genes nadk2 and NADK2 of gene NADK2 which represents for the wild type gene At1G21640, stoma opening and closing processes are induced by abscisic acid which is regulated and controlled by the gene KADK2; in other words, the gene NADK2 is related to plant drought stress in terms of responses; thus, to the over-expression of the gene NADK2, it is possible to culture a new variety of drought-resistant plants.

Description

Arabidopsis At1G21640 gene new opplication in plant drought
Technical field
The invention belongs to gene engineering technology field, be specifically related to the patent application of arabidopsis At1G21640 gene new opplication in plant drought。
Background technology
Arid is the main environment stress factors that terrestrial plant frequently suffers from, and has a strong impact on the growth promoter of plant and normal physiological and biochemical procedure, reduces crop yield and quality, brings serious harm to agricultural production。
Plant defines the mechanism resisting drought stress in long-term evolution process, and wherein the mechanism of most critical is by regulating and controlling the evaporation of the closing control internal water of blade epidermis pore, because the moisture of more than 90% is lost in air by pore in plant。Pore is made up of a pair guard cell, and during guard cell's imbibition, pore is opened, moisture loss；During guard cell's dehydration, stomatal closure, moisture is retained in plant, and therefore plant prevents moisture from losing in a large number by closing pore, resists drought stress。Research shows, plant stomata is closed and is limited mainly by hormone abscisic acid (ABA) regulation and control。When plant suffers drought stress, ABA synthesis dramatically increases, blade accumulates, be combined with ABA receptor, many signal transduction compositions in regulation and control Stomacal guard cell, cause guard cell's plasma membrane reversing, anion and potassium ion in guard cell's kytoplasm is finally made to flow in a large number outside kytoplasm, along with the outflow of ion, moisture outflow, stomatal closure in guard cell。
Taking place frequently with drought along with whole world freshwater resources reduce day by day, cultivating drought-enduring plant new varieties will become following reply drought stress, improves the important channel of crop yield and quality。The fast development of technique for gene engineering, the continuous of new Drought-tolerant gene find and excavate, and will establish solid foundation for cultivation drought-enduring plant new varieties。Therefore, excavating or qualification to the drought-enduring New function of existing gene of new Drought-tolerant gene, significant for cultivating new drought-enduring plant new varieties。
Summary of the invention
Present invention aim at providing arabidopsis At1G21640 gene new opplication in plant drought, thus for cultivating the possibility that drought-enduring plant new varieties provide new。
The detailed technology scheme that the present invention takes is described below。
Arabidopsis At1G21640 gene new opplication in plant drought, described arabidopsis At1G21640 gene is relevant to plant drought function, and after by At1G21640 gene delection, mutant plants promotes for the sensitivity of drought stress；And after by At1G21640 gene overexpression, mutant plants obtains enhancing for the toleration of drought stress；
Further investigations have shown that, described At1G21640 gene is by participating in the ABA drought resisting reaction inducing the process adjustment plant of stomatal closure。
It is contemplated that carry out At1G21640 gene in other plant converting or after overexpression, drought-enduring plant new varieties can be obtained。
For arabidopsis At1G21640 gene (NADK2Gene,Nicotinamideadeninedinucleotidekinase2), there are some researches show that one NAD kinases of this gene code is positioned plant chloroplast；This NAD is kinase catalytic is changed into NADP (H) by NAD (H), has important function at Chlorophyll synthesis and anti-oxidation protection chloroplast and when realizing energy conversion by Zeaxanthin cycle in photosynthetic electron transfer。But about whether this gene and coded kinases thereof regulate and control drought stress responsing reaction and stomatal closure then without relevant report。
The present invention to wild type (WT),NADK2Afunction mutantnadk2、NADK2The gene overexpression plant serial physiological Study in drought stress environment finds,NADK2The stomatal closure that gene is induced to ABA is relevant, namelyNADK2Gene is relevant to the drought stress response of plant, this with known forNADK2The function cognition of gene has relatively big difference。Meanwhile, it is applicant's understanding that forNADK2Correlated inheritance operation (overexpression of gene) of gene, can for cultivating the possibility that new drought-enduring plant new varieties provide new。
Accompanying drawing explanation
Fig. 1 is the arabidopsis wild type (WT), the NADK2 afunction mutant that adopt reverse transcriptional PCR detection to obtainnadk2、NADK2Plant (includes gene overexpressionOE1WithOE2) inNADK2The expression of gene；
Fig. 2 be arabidopsis wild type (WT),NADK2Afunction mutantnadk2、NADK2Plant (includes gene overexpressionOE1WithOE2) phenotype relevant before and after Osmotic treatment, wherein A is the phenotype of each plant before Osmotic treatment, and B is the phenotype of each plant after Osmotic treatment, and C is the phenotype of each plant after Drying and rewatering；
Fig. 3 be for arabidopsis wild type (WT),NADK2Afunction mutantnadk2、NADK2Plant (includes gene overexpressionOE1WithOE2) blade surface temperature detection situation, wherein left figure is far infrared imagery figure, and right figure is leaf table temperature statistics situation, and in figure, " * " represents have significant difference；
Fig. 4 be for arabidopsis wild type (WT),NADK2Afunction mutantnadk2、NADK2Plant (includes gene overexpressionOE1WithOE2) blade table leather strap stomatal aperture after variable concentrations ABA processes, in figure, " * ", " * * " represent have significant difference。
Detailed description of the invention
Technical scheme is described in further detail as follows below in conjunction with embodiment。Before introducing specific embodiment, part raw material involved in the present invention and related reagent situation are briefly introduced explanation as follows。
Biomaterial:
(WT, the ecotype is arabidopsis wild typeCol-0, lower with), NADK2 afunction mutantnadk2Obtain from arabidopsis germ plasm resource center；
NADK2Gene overexpression plantOE1WithOE2Obtained by transgenic method by this seminar, concrete grammar is: extract total serum IgE from Arabidopsis thaliana Seedlings with Trizol test kit, cDNA is obtained with RNA Reverse Transcriptase kit reverse transcription, then with cDNA for template, with following primer SP1 and SP2(two primer 5 ' end contain Xbal and SalI restriction enzyme site sequence respectively) and PCR instrument expand obtainNADK2Gene order, with Xbal and SalI double digestionNADK2Gene order, leakage of electricity is swum, and reclaims genetic fragment；Extracting plasmid DNA from the bacterial isolates carrying pSuper1300 overexpression vector, by this plasmid DNA of Xbal and SalI double digestion, the fragment after enzyme action is reclaimed in leakage of electricity swimming simultaneously；Genetic fragment and carrier segments T4DNA ligase are connected, and transform bacteria competent cell, build and started by 35S promoterNADK2The overexpression vector of gene；NADK2After gene sequencing is correct, with the vector Agrobacterium competent cell built, it is thus achieved that carryNADK2The overexpression vector of gene, then with this Agrobacterium-mediated Transformation arabidopsis alabastrum, it is thus achieved that arabidopsis transgenic plant, finally obtains F3 for seed, for subsequent experimental；
The sequence of above-mentioned primer SP1 and SP2 is as follows:
SP1 sequence: 5 '-CTGTCTAGAATGTTCCTATGCTTTTGCCCT-3 ',
SP2 sequence: 5 '-TTCGTCGACTCATCAGAGAGCCTTTGATCAAGAC-3 '。
Related reagent:
Trizol test kit, Invitrogen company；
RNA Reverse Transcriptase kit, Promega company；
T4DNA ligase, Promega company；
Relevant primer sequence is synthesized by calm and peaceful biotechnology (Beijing) company limited of Sino-U.S., clone'sNADK2Gene is also checked order by calm and peaceful biotechnology (Beijing) company limited of Sino-U.S.。
Related equipment:
PCR instrument MyCycler, Bio Rad Laboratories；
Far infrared imagery instrument ThermaCAMSC3000, power & light company of the U.S.；
TE-300 optical microscope, Nissan Motor of Japan。
Embodiment 1
For arabidopsis wild type (WT),NADK2Afunction mutantnadk2、NADK2Gene overexpression (includesOE1WithOE2) plant, inventor is first against within corresponding plantNADK2The expression of gene detects, and related experiment process is briefly discussed below。
Arabidopsis seed dibbling is grown two weeks in MS culture medium (containing 3% sucrose, 0.6% agar, pH5.8) medium temperature chamber, hot-house culture condition: temperature 18 DEG C ~ 21 DEG C, humidity 50 ~ 70%, illumination every day 12 hours, dark 12 hours, the about 100 μm of olm of light intensity–2s–1。
Take the growth Arabidopsis leaf of two weeks, extract blade total serum IgE initially with Trizol test kit, then adopt the RNA Reverse Transcriptase kit total serum IgE to extracting to carry out reverse transcription, obtain cDNA；With the cDNA that obtains for template, carry out reverse transcriptional PCR, to determineNADK2Expression, withActin2Expression as comparison。
Pcr amplificationNADK2During gene, relevant primer sequence is as follows:
ForNADK2Gene:
Forward primer is 5 '-AATAATAGTGGTTCCTCCTCGG-3 ',
Downstream primer is 5 '-GATCTTAGAAAGGTATGGGTTGG-3 '；
ForActin2Gene:
Forward primer is 5 '-TGTGCCAATCTACGAGGGTT-3 ',
Downstream primer is 5 '-TGCTCATACGGTCAGCGATA-3 '。
Correlated results is as shown in Figure 1。It can be seen that at NADK2 afunction mutantnadk2In, it does not have relevant band, explanation detectednadk2In lackNADK2The expression of gene；And from the luminance contrast of band it can be seen thatNADK2Plant (includes gene overexpressionOE1WithOE2) in,NADK2Gene expression amount dramatically increases than wild type (WT)。
Embodiment 2
The present embodiment is mainly introduced for arabidopsis wild type (WT), NADK2 afunction mutantnadk2、NADK2Plant (includes gene overexpressionOE1WithOE2) Relevant phenotype situation of change in Osmotic treatment, related experiment process is briefly discussed below。
Arabidopsis seed dibbling is grown 10 days in MS culture medium (containing 3% sucrose, 0.6% agar, pH5.8) medium temperature chamber, hot-house culture condition: temperature 18 DEG C ~ 21 DEG C, humidity 50 ~ 70%, illumination every day 12 hours, dark 12 hours, the about 100 μm of olm of light intensity–2s–1；Then cultivating 3 weeks in seedling replanting to the Nutrition Soil of tray, Nutrition Soil is pressed the preparation of 1:1 mass ratio, greenhouse experiment: temperature 18 DEG C ~ 21 DEG C, humidity 50 ~ 70%, illumination every day 14 hours, dark 10 hours, about 100 ~ 120 μm of olm of light intensity by humus and Vermiculitum–2s–1；The Seedling just transplanted waters permeable, rewaters, hereafter watered a water per every about 3 days after one week。
In Fig. 2 shown in A figure, grow in Nutrition Soil 3 weeks WT,nadk2AndOE1WithOE2Plant strain growth is normal。Now, keeping other growth conditions constant, stop supplying water, when the 6th day, plant leaf starts to wilt, and when the 10th day, all of blade is all wilted, in practical situation such as Fig. 2 shown in B figure。After stopping water supply 10 days, start again to recover normal water supply, in result such as Fig. 2 shown in C figure, after resuming water supply 2 days,NADK2Afunction mutantnadk2Plant all withered, wild type (WT) and NADK2 gene overexpression plant (including OE1 and OE2) have then recovered sign of life preferably, and the growing way of NADK2 gene overexpression plant is significantly better than wild type (WT)。
Summary experimental result it can be seen thatNADK2Gene (At1G21640 gene) is relevant to the drought resistance of plant, will can significantly improve the drought resistance of plant after this gene overexpression。
Embodiment 3
The present embodiment is mainly introduced for arabidopsis wild type (WT), NADK2 afunction mutantnadk2、NADK2Plant (includes gene overexpressionOE1WithOE2) growth course Leaf surface temperature change detection case。
The variations in temperature of blade surface is closely related with leaves water loss speed, and heat scatters and disappears with leaf water and scatters and disappears, the blade that therefore dehydration is fast, and leaf table temperature is low, the blade that dehydration is slow, and leaf table temperature is high。
Arabidopsis seed dibbling is grown 10 days in MS culture medium (containing 3% sucrose, 0.6% agar, pH5.8) medium temperature chamber, hot-house culture condition: temperature 18 DEG C ~ 21 DEG C, humidity 50 ~ 70%, illumination every day 12 hours, dark 12 hours, the about 100 μm of olm of light intensity–2s–1；Then being moved on to by seedling in the Nutrition Soil of tray and cultivate, Nutrition Soil is mixed thoroughly by 1:1 mass ratio by humus and Vermiculitum, greenhouse experiment: temperature 18 DEG C ~ 21 DEG C, humidity 50 ~ 70%, illumination every day 14 hours, dark 10 hours, about 100 ~ 120 μm of olm of light intensity–2s–1；The Seedling just transplanted waters permeable, waters, watered a water per every about 3 days after one week。
After seedling length to 3 week, far infrared imagery instrument ThermaCAMSC3000 is utilized to obtain thermograph。Thermograph adopts software FLIRQuickReport1.2sp2 to be analyzed and leaf table temperature statistics。
Result is as it is shown on figure 3, NADK2 afunction mutantnadk2Leaf table temperature significantly lower than wild type (WT), andNADK2Plant (includes gene overexpressionOE1WithOE2) leaf table temperature then slightly above wild type (WT), these it is shown thatnadk2Leaves water loss is significantly faster than that wild type WT, andNADK2Plant (includes gene overexpressionOE1WithOE2) leaves water loss be then slower than wild type (WT)。
Embodiment 4
The present embodiment is mainly introduced for arabidopsis wild type (WT), NADK2 afunction mutantnadk2、NADK2Plant (includes gene overexpressionOE1WithOE2) in ABA effect air holes aperture situation of change, related experiment is briefly discussed below。
The cultural method of arabidopsis Seedling is with embodiment 2, when Seedling grows to 3 ~ 4 weeks, take fully deployed spire, tear the epidermis bar taking vacuum side of blade, the mesophyll of epidermis adhesive tape is brushed off gently with brush pen, epidermis bar is totally submerged in Mes-KCl(pH6.15) in buffer, it is then placed in exsiccator, at 200 μm of olm–2s–1Irradiating 2.5 hours under the white light of intensity of illumination, make pore completely open, with being measured microscopically stomatal aperture, stomatal aperture now, as comparison, is designated as the front stomatal aperture of process。Then being separately added into the ABA that working concentration is 1 μM, 10 μMs and 50 μMs in Mes-KCl buffer, identical intensity of illumination is after lower 1 hour, and under microscope, (40 ×) take pictures pore, and add up stomatal aperture。
Arid may result in plant dehydration and even wilts。Under drought condition, plant leaf blade cell ABA Rapid Accumulation, promote stomatal closure, to reduce the moisture transpiration speed of plant, thus resisting drought stress。The transpiration divided due to stomatal aperture change adjusting plant water is scattered and disappeared, thus the change of ABA effect air holes aperture can reflect the drought resistance of plant, and ABA processes posterior spiracle quick closedown, and pore dehydration is slow, and plant drought resistance is strong, otherwise then plant drought resistance is poor。The stomatal aperture change that namely the present embodiment is to induce under variable concentrations ABA treatment conditions reflectsNADK2Gene is for the response condition of plant arid。
As shown in Figure 4, after ABA processes, stomatal aperture significantly reduces in statistical result, but NADK2 afunction mutantnadk2Stomatal aperture varies less, explanationnadk2Stomatal closure insensitive to ABA induction, namelynadk2Arid is insensitive, andNADK2Plant (includes gene overexpressionOE1WithOE2) stomatal closure of ABA induction is more sensitive, it was shown that NADK2 gene can improve the drought resistance of plant, has important function in response drought resisting Stress responses process。

Claims (3)

1. arabidopsis At1G21640 gene new opplication in plant drought, it is characterised in that described arabidopsis At1G21640 gene is relevant to plant drought function, after by At1G21640 gene delection, mutant plants promotes for the sensitivity of drought stress；And after by At1G21640 gene overexpression, mutant plants obtains enhancing for the toleration of drought stress。
2. arabidopsis At1G21640 gene new opplication in plant drought as claimed in claim 1, it is characterised in that At1G21640 gene is by participating in the drought resisting reaction of the stomatal closure process adjustment plant of abscisic acid induction。
3. utilize the method that At1G21640 gene cultivates drought-enduring plant new varieties, it is characterised in that At1G21640 gene is carried out overexpression。
CN201610246041.8A 2016-04-20 2016-04-20 New application of Arabidopsis Thaliana gene At1G21640 in plant drought resistance Pending CN105695488A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610246041.8A CN105695488A (en) 2016-04-20 2016-04-20 New application of Arabidopsis Thaliana gene At1G21640 in plant drought resistance

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610246041.8A CN105695488A (en) 2016-04-20 2016-04-20 New application of Arabidopsis Thaliana gene At1G21640 in plant drought resistance

Publications (1)

Publication Number Publication Date
CN105695488A true CN105695488A (en) 2016-06-22

Family

ID=56217145

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610246041.8A Pending CN105695488A (en) 2016-04-20 2016-04-20 New application of Arabidopsis Thaliana gene At1G21640 in plant drought resistance

Country Status (1)

Country Link
CN (1) CN105695488A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108728480A (en) * 2018-04-28 2018-11-02 河南大学 Application of the At3g16910 genes in terms of cultivating drought-resistant crops
CN114532336A (en) * 2022-01-19 2022-05-27 浙江大学 Application of abscisic acid as negative regulation factor in regulating synthesis of 5-hydroxytryptamine in plants
CN117187275A (en) * 2023-11-08 2023-12-08 清华大学 Expression system, construction method and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101748143A (en) * 2009-12-18 2010-06-23 河南大学 Application of AtMPK6 to plant drought combatting aspect
CN101781660A (en) * 2009-01-21 2010-07-21 河南大学 Application of arabidopsis gene IAR1 in aspects of iron nutrient and drought resistance
CN104263735A (en) * 2014-07-31 2015-01-07 河南大学 Novel application of arabidopsis TTG1 genes to plant salinity-resisting and drought-resisting aspects

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101781660A (en) * 2009-01-21 2010-07-21 河南大学 Application of arabidopsis gene IAR1 in aspects of iron nutrient and drought resistance
CN101748143A (en) * 2009-12-18 2010-06-23 河南大学 Application of AtMPK6 to plant drought combatting aspect
CN104263735A (en) * 2014-07-31 2015-01-07 河南大学 Novel application of arabidopsis TTG1 genes to plant salinity-resisting and drought-resisting aspects

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
LEE HUNT等: "NAD – new roles in signalling and gene regulation in plants", 《NEW PHYTOLOGIST》 *
MAO-FENG CHAI等: "NADK2, an Arabidopsis chloroplastic NAD kinase, plays a vital role in both chlorophyll synthesis and chloroplast protection", 《PLANT MOLECULAR BIOLOGY》 *
XIANG WANG等: "Identification and Functional Analysis of the NADK Gene Family in Wheat", 《PLANT MOL BIOL REP》 *
康绍忠主编: "《农业水土工程概论》", 30 September 2007 *
王君晖等: "植物细胞的抗脱水反应及其重要蛋白产物", 《细胞生物学杂志》 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108728480A (en) * 2018-04-28 2018-11-02 河南大学 Application of the At3g16910 genes in terms of cultivating drought-resistant crops
CN114532336A (en) * 2022-01-19 2022-05-27 浙江大学 Application of abscisic acid as negative regulation factor in regulating synthesis of 5-hydroxytryptamine in plants
CN114532336B (en) * 2022-01-19 2023-03-14 浙江大学 Application of abscisic acid as negative regulation factor in regulating synthesis of 5-hydroxytryptamine in plants
CN117187275A (en) * 2023-11-08 2023-12-08 清华大学 Expression system, construction method and application thereof
CN117187275B (en) * 2023-11-08 2024-03-12 清华大学 Expression system, construction method and application thereof

Similar Documents

Publication Publication Date Title
CN107435047A (en) In a kind of plant phosphorus signal network Tolerant to low P key gene GmPHR25 and its with application
CN101006768B (en) Agrobacterium-mediated Sophora japonica transgene and tissue-culturing rapid propagation method
CN104195150A (en) Application of arabidopsis glycosyl transferase gene UGT79B2 in improving salt resistance and drought resistance of plants
CN105695488A (en) New application of Arabidopsis Thaliana gene At1G21640 in plant drought resistance
CN104450740B (en) A kind of alfalfa MsWRKY33 transcription factor and encoding proteins, preparation method and application
CN102174568B (en) Method for in-situ transgenosis of corn mature embryo
CN105294847A (en) Stress tolerance-related protein of plants and encoding gene and application of stress tolerance-related protein
CN108624596A (en) It is a kind of regulation and control Legume nodule growth gene GmSPX5 and its application
CN108998472A (en) A method of cadmium-resistant vegetable ability is improved using ramie BnXTH1 gene
CN106755065A (en) A kind of screening technique of brassinosteroid biosynthesis gene DWF5 mutant
CN103602688B (en) Helianthus tuberosus L. Na<+>/H<+> reverse transport protein genes HtNHX1 and HtNHX2 and use thereof
CN103898155B (en) Particle gun is utilized to obtain method and the special culture media thereof of transgenic wheat
CN104726488A (en) Method for culturing stress-resistance herbicide-resistance transgenic aerobic rice
CN103343139B (en) Novel method for enhancing drought resistance of plants
CN103911386A (en) Artificial fusion gene for improving stress tolerance of plants
CN109161537B (en) Application of TPPI gene in regulating and controlling plant stomata opening and improving plant drought resistance
CN109082428B (en) The application of rice stomatal opening type potassium-channel gene OsK2-1 and its expression vector
CN104498508A (en) Wheat introgression line response abiotic stress regulation gene TaGBF and application
Kordrostami et al. Consequences of water stress and salinity on plants/crops: physiobiochemical and molecular mitigation approaches
TWI624544B (en) A process for increasing growth of hydroponic grown plants under salt stress
CN105586347A (en) Tobacco drought response gene NtRDP1 as well as encoded protein and application thereof
CN104498512A (en) Application of arabidopsis calcium ion-dependent protein kinase gene AtGPK1 in regulation and control of stomatal movement and plant drought resistance
CN103773801A (en) Application for breeding transgenic water-saving and drought-resistance plant by using aspen ABA (Abscisic Acid) receptor PtPYRL gene
CN109810984A (en) A kind of relevant Sesame SiGolS6 of drought resisting and its application
CN114107333B (en) Application of barley receptor kinase HvSERK1 in root hair growth

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20160622