CN105687291A

CN105687291A - Balsam pear total saponin extraction technology

Info

Publication number: CN105687291A
Application number: CN201410673826.4A
Authority: CN
Inventors: 雷印平
Original assignee: Individual
Current assignee: Individual
Priority date: 2014-11-24
Filing date: 2014-11-24
Publication date: 2016-06-22

Abstract

The invention discloses a balsam pear total saponin extraction technology. The extraction technology comprises the following steps: drying fresh balsam pear, crushing dried balsam pear, sieving the crushed balsam pear, degreasing the sieved balsam pear with petroleum ether, accurately weighing 2.5g of treated balsam pear, placing the weighed treated balsam pear in a 250ml iodine flask, and extracting the treated balsam pear with an extraction solvent 60-80% ethanol at an extraction temperature of 50-70DEG C under a solid/liquid ratio of 1:10-1:16 for 2-4h through a water bath oscillation extraction method; and taking the obtained extract, filtering the extract, extracting filter residues, filtering the obtained extract, mixing two obtained filtrates, carrying out reduced pressure concentration on the obtained filtrate mixture, and carrying out vacuum drying to obtain a crude saponin product. Balsam pear total saponins extracted by adopting the technology have highest yield and best purity.

Description

Total Saponins from Bitter Melon extraction process

Technical field

The present invention relates to total Saponins from Bitter Melon and extract field, be specially a kind of total Saponins from Bitter Melon extraction process。

Background technology

Fructus Momordicae charantiae is cucurbitaceous plant, and the name of Fructus Momordicae charantiae is first appeared in " the southern regions of the Yunnan Province book on Chinese herbal medicine "。Fructus Momordicae charantiae originates in tropical and subtropical region, incoming China during the Southern Song Dynasty, has plantation at south China more, and the provinces and regions such as Guangxi, Guangdong, Yunnan, Fujian are in the majority, and the Northeast's cultivated area in recent years expands gradually。Modern medicine study finds, the Momordica charantial contained in Fructus Momordicae charantiae has blood sugar lowering, antioxidation, raising immunocompetence, reduces the physiological functions such as cholesterol, antitumor and anti AIDS virus。Except as medicine, saponin is also used as superior cosmetics and food additive, as having a wide range of applications in cosmetics and food industry as antibacterial, antioxidant, foaming agent etc., and is applied to chemical industry as surfactant。Therefore, carry out the research to total Saponins from Bitter Melon extraction process to be of great practical significance。

Summary of the invention

The present invention is directed to above weak point, it is provided that a kind of total Saponins from Bitter Melon extraction process, adopt the total Saponins from Bitter Melon yield that the method is extracted the highest, purity is best。

The technical solution adopted for the present invention to solve the technical problems is: a kind of total Saponins from Bitter Melon extraction process, described extraction step is: fresh bitter is dried, pulverize, sieve after through petroleum ether degreasing, accurately weigh the Fructus Momordicae charantiae 2.5g processed in 250ml iodine flask, water-bath mechanical shaking extraction method is adopted to extract, Extraction solvent is 60%-80% ethanol, Extracting temperature is 50-70 degree, and solid-to-liquid ratio is 1:10-1:16, and extraction time is 2-4h；Take out and filter, by filtering after filtering residue secondary lixiviate, merge twice gained filtrate, after carrying out concentrating under reduced pressure, vacuum drying, namely obtain thick saponin product。

A kind of total Saponins from Bitter Melon extraction process, described extraction step is: fresh bitter is dried, pulverize, sieve after through petroleum ether degreasing, accurately weigh the Fructus Momordicae charantiae 2.5g processed in 250ml iodine flask, water-bath mechanical shaking extraction method is adopted to extract, Extraction solvent is 70% ethanol, Extracting temperature is 60 degree, and solid-to-liquid ratio is 1:14, and extraction time is 3h；Take out and filter, by filtering after filtering residue secondary lixiviate, merge twice gained filtrate, after carrying out concentrating under reduced pressure, vacuum drying, namely obtain thick saponin product。

The invention has the beneficial effects as follows: adopt ethanol extraction, pollution-free, adopt water-bath mechanical shaking extraction, extraction process is simple, and experiment appliance is easy to get。

Detailed description of the invention

Embodiment 1

A kind of total Saponins from Bitter Melon extraction process, described extraction step is: fresh bitter is dried, pulverize, sieve after through petroleum ether degreasing, accurately weigh the Fructus Momordicae charantiae 2.5g processed in 250ml iodine flask, water-bath mechanical shaking extraction method is adopted to extract, Extraction solvent is 60% ethanol, Extracting temperature is 50 degree, and solid-to-liquid ratio is 1:10, and extraction time is 2h；Take out and filter, by filtering after filtering residue secondary lixiviate, merge twice gained filtrate, after carrying out concentrating under reduced pressure, vacuum drying, namely obtain thick saponin product。

Embodiment 2

A kind of total Saponins from Bitter Melon extraction process, described extraction step is: fresh bitter is dried, pulverize, sieve after through petroleum ether degreasing, accurately weigh the Fructus Momordicae charantiae 2.5g processed in 250ml iodine flask, water-bath mechanical shaking extraction method is adopted to extract, Extraction solvent is 80% ethanol, Extracting temperature is 70 degree, and solid-to-liquid ratio is 1:16, and extraction time is 4h；Take out and filter, by filtering after filtering residue secondary lixiviate, merge twice gained filtrate, after carrying out concentrating under reduced pressure, vacuum drying, namely obtain thick saponin product。

Embodiment 3

Claims

1. a total Saponins from Bitter Melon extraction process, it is characterized in that: described extraction step is: fresh bitter is dried, pulverize, sieve after through petroleum ether degreasing, accurately weigh the Fructus Momordicae charantiae 2.5g processed in 250ml iodine flask, water-bath mechanical shaking extraction method is adopted to extract, Extraction solvent is 60%-80% ethanol, Extracting temperature is 50-70 degree, and solid-to-liquid ratio is 1:10-1:16, and extraction time is 2-4h；Take out and filter, by filtering after filtering residue secondary lixiviate, merge twice gained filtrate, after carrying out concentrating under reduced pressure, vacuum drying, namely obtain thick saponin product。

2. a total Saponins from Bitter Melon extraction process according to claim 1, it is characterized in that: described extraction step is: fresh bitter is dried, pulverize, sieve after through petroleum ether degreasing, accurately weigh the Fructus Momordicae charantiae 2.5g processed in 250ml iodine flask, water-bath mechanical shaking extraction method is adopted to extract, Extraction solvent is 70% ethanol, Extracting temperature is 60 degree, and solid-to-liquid ratio is 1:14, and extraction time is 3h；Take out and filter, by filtering after filtering residue secondary lixiviate, merge twice gained filtrate, after carrying out concentrating under reduced pressure, vacuum drying, namely obtain thick saponin product。