CN105684701A - Method for improving ryegrass growth by adding crab shell powder in matrix - Google Patents

Method for improving ryegrass growth by adding crab shell powder in matrix Download PDF

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Publication number
CN105684701A
CN105684701A CN201610065304.5A CN201610065304A CN105684701A CN 105684701 A CN105684701 A CN 105684701A CN 201610065304 A CN201610065304 A CN 201610065304A CN 105684701 A CN105684701 A CN 105684701A
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China
Prior art keywords
eriocheir sinensis
carapax eriocheir
shell powder
plant
sinensis powder
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CN201610065304.5A
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Chinese (zh)
Inventor
赵树兰
多立安
闫雪
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Tianjin University
Tianjin Normal University
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Tianjin Normal University
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Priority to CN201610065304.5A priority Critical patent/CN105684701A/en
Publication of CN105684701A publication Critical patent/CN105684701A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general

Abstract

The invention discloses application of crab shell powder in increasing the aboveground biomass of ryegrass and improving primary growth of a tall fescue turf. The adding amount of the crab shell powder is 1%-1.5% (W/w). Experimental results show that by adding the crab shell powder of the different proportions into a ryegrass matrix, the crab shell powder obviously increases the fresh weight and the dry weight of the aboveground biomass of the ryegrass, and the dry weight of second clipping grass especially shows the significant difference. In addition, by adding the crab shell powder, increasement of the plant height can be promoted, and the significantly promoting effect on the plant height of the second clipping grass is especially achieved.

Description

A kind of method adopting substrate interpolation Carapax Eriocheir sinensis powder to improve Ryegrass Growth
Technical field
The invention belongs to environmental protection technical field, relate to a kind of method adopting substrate interpolation Carapax Eriocheir sinensis powder to improve Ryegrass Growth。
Background technology
Urban lawn planting system is the ecological engineering integrating the several functions such as ecological regulation and control, beautification of landscape, CRUX and entertainment。It is the important component part of urban Compound greening system, not only plays an important role in maintaining compound city ecology system balance, and the view that it brings is that other construction project of city is irreplaceable with project with landscaping effect。Why lawn plays important role in urban ecological system, has special ecosystem service function owing to it。The value of ecosystem service of turf establishment system is mainly: lawn plant can pass through to reduce Effective radiation and absorbing carbon dioxide, alleviates " urban heat island " effect;The oxygen of photosynthesis of plant release, can improve urban air quality, Gas regulation humidity;Plant energy absorption and sorption and the pollutant in degraded environment;Also can move for people, have a rest, stroll about or have a rest and enjoy life offer activity space on lawn;View specific to lawn and landscaping effect, bring the impression of U.S.。Now, turf establishment system builds has become one of important content of must take in modern city ecological construction, and lawn greening area and quality have become the important objective criterion evaluating city environmental quality。Additionally, turf establishment system is also the window showing modern city social development levels and economic strength, it improving City Investment Environment, attract foreign investment, promote tourism etc. in all play an important role。
Domestic and international turf establishment system builds present situation and can be summarized as: first, and external per capita green grassland area is relatively big, and area as per capita in Washington is 45.7m2, human relations pier 30.4m2, rubbish 70.5m may be trained2, Warsaw 70.0m2, Moscow 44.0m2。Secondly, turf establishment had become a big efficient ecological industry of a lot of developed country already, and output value growth rate is much larger than the economic growth rate same period, as U.S.'s annual value of production grows at top speed with 18%;Lawn Industry is number two in U.S.'s crop industry, and its same aviation, electronics etc. become one of country's ten big industries in the lump。Further, turf establishment industry has also driven the fast development of the related industry such as machine-building, community service。
Compared with advanced international standard, China's turf establishment system builds exists gap in all many-sides such as planting scale, quality, management and research and development。From scale, city of the world's Indices of Ecological is green areas of average per-person share 20m2, and a lot of city of China is less than 3m2。In recent years, along with the high speed development of China's economic, the demand of various function turf establishment system is grown with each passing day by city。Expert estimates, coming 10 years China lawn practitioner, up to 4,000,000, will exceed forestry population when the time comes, and the comprehensive service function of turf establishment system also will be continuously available lifting。The family of lawn enterprise more than 400 of state-owned 1,000,000 yuan of output values at present, only Beijing suburb is engaged in the company of sod production and is just reached more than 2000 places with peasant household。Visible, in modern industry chain, urban lawn planting system construction industry has become a big ecological industry of great vigor。
In turf establishment materials application, floor space grass seed, brood body carry material and the selection of substrate packing material etc. that uses for various purposes is particularly subject to the great attention of people。Should say that turf establishment way choice is directly connected to the success or failure of turf establishment。Certainly, turf establishment can Cheng Ping rapidly, and produce a desired effect, additionally depend on features of Ecological Environment and the management technique level after Cheng Ping to a great extent。
Although lawn greening system has the advantage that many worth people speak approvingly of, but this at all can not cover the shortcoming existing for lawn greening, dependence on import as long-term in grass seeds, and according to incompletely statistics, often the grass seeds quantity of China's import every year is up to more than 7000 tons;Therefore in this case, research and development improve the vigor of turf establishment initial stage seed, promote the technology of lawn plant seed planting efficiency, enhancing lawn plant primary growth gesture, will be a very significant job。
Rye grass also plays an important role in improvement ecological environment, the ecological agriculture and Sustainable Socioeconomic Development etc. as important lawn plant。Mo Zhenghai studies and discusses rye grass application in ecology, its main Ecology Action has water and soil conservation function, purifies sewage function and the function of improvement soil, rye grass energy repairing polluted soil, improve soil physical properties, improve soil permeability and promote the accumulation etc. of the soil organism。The seed that Lan Jian et al. gathers in the crops with English ryegrass different times is for object of study, show that the growth of seedling that mass of 1000 kernel is big is preponderated, and the quality of seedling is good, also find that its germination percentage of seed, emergence rate and planting percent that mass of 1000 kernel is big are also high, difference between three is only small, and in significant positive correlation between three。The development and utilization that these researchs are all rye grass from now on provides foundation。
The lake, inland river of China is numerous, and marine site is extensive, and shellfish resources is very abundant, and wherein the kind of Eriocheir sinensis is up to kind more than 600, can produce substantial amounts of Carapax Eriocheir sinensis every year, and Carapax Eriocheir sinensis is the living resources of a kind of preciousness。According to the study, Carapax Eriocheir sinensis is mainly composed of chitin, calcium carbonate, protein, calcium phosphate, aromatic substance, fat-soluble carotene and fatsoluble vitamin etc., chitin therein is also known as chitin, chitin, crab shell chitosan, it is natural polymer the abundantest except cellulose on the earth, is also the natural organic-compound that in nature, beyond isolating protein, quantity is maximum。According to estimates, about 100,000,000 tons are have by biosynthetic carapace every year, it has also become the regenerated resources treasure-house that the mankind are inexhaustible。Chitin can obtain the various chitin derivativs with special nature by chemical method under chitin enzyme effect。Such as chitosan (chitosan), shuttle ethyl chitin, shuttle methyl chitosan (NCMC), glucosamine, chitin oligosaccharide etc.。Wherein chitosan is also known as chitosan, and chemical name is (Isosorbide-5-Nitrae)-2-amino-2-deoxidation-callose, is chitin de-second phthalidyl and product of changing under the effect of highly basic, and is the topmost derivant of chitin。Chitosan has the function of many uniquenesses, for instance degraded nontoxic, easy, have good bioaffinity etc.。In recent years, numerous in the research of agriculture field about chitosan, it was found that chitosan has the function of plant growth regulator, the yield and quality etc. of the growth that can regulate crop, the growth promoting rhizome, the resistance increasing crop and raising crop acts on。It shows as in regulating crop growth and can improve wheat yield and seed protein content etc.。Secondly, there are some researches show chitin, chitosan application agriculturally show as can as degradability mulch film, plant growth regulator, make fertilizer, pesticide and make feed additive etc.。Yu Mingge et al. has inquired into the chitosan adjustment effect to the Physiology and biochemistry of cucumber seedling with cucumber seeds and seedling for investigation of materials, it was shown that suitable concentration chitosan can promote cucumber cotyledons weight, improves its chlorophyllous content, and then promotes the growth promoter of plant。Also there are some researches show that chitosan can significantly improve the germination percentage of seed, reinforcing alpha-diastatic activity, and then promote the sprouting of seed and the Biomass of seedling, the photosynthetic performance of plant can be improved, strengthen the ability of its carbon assimilation, thus increasing weight in wet base and the dry weight of seedling, moreover it is possible to improve the soluble protein of plant and the content etc. of soluble sugar。It addition, chitosan is because containing the existence of a large amount of free ammonias in its molecular structure, therefore it has some special physico-chemical property and physiological functions, and it also has great application prospect in all many-sides such as food, medicine, cosmetics, environmental protection。But, chitosan function in other respects needs further to inquire into research。
It is directly appended in substrate about Carapax Eriocheir sinensis powder both at home and abroad the report of ozone deplation is less。And have no Carapax Eriocheir sinensis powder and regulate the correlation technique report of Ryegrass Growth。Material is regulated for lawn matrix herein, it is intended that utilizing Carapax Eriocheir sinensis powder to reach to improve the purpose of Ryegrass Growth performance, thus finding the Carapax Eriocheir sinensis powder the suitableeest adding proportion to Ryegrass Growth, providing new approach for Carapax Eriocheir sinensis recycling with Carapax Eriocheir sinensis powder。
Summary of the invention
The invention discloses a kind of method adopting substrate interpolation Carapax Eriocheir sinensis powder to improve Ryegrass Growth。Particularly Carapax Eriocheir sinensis powder is improving rye grass ground biomass, improves the application of the primary growth aspect of tall fescue turf;Wherein the addition of Carapax Eriocheir sinensis powder is 1-1.5%(W/w), it is preferable that 1.5%。Invention also discloses the application that Carapax Eriocheir sinensis powder promotes the growth aspect of rye grass plant plant height simultaneously。Particularly Carapax Eriocheir sinensis powder application in promoting rye grass eldish dry weight。
More detailed description of the present invention is as follows:
1 develops materials and methods
1.1 materials
The lawn plant English ryegrass (LoliumperenneL) selecting northern China relatively common is experiment material, mass of 1000 kernel 3.17g。Being taken from Tianjin Normal University campus the topsoil of sample plot 0-20cm for examination soil, the soil texture is sandy soil, and pH is about 7.44, the content of organic matter is 4.68%, and total nitrogen content is 0.21%, available phosphorus content 22.03mg/kg, saturation moisture content is 0.58mL/g, and unit weight is 0.87g/cm3。Carapax Eriocheir sinensis selects sea-crab, is first cleaned up by Carapax Eriocheir sinensis, dries, pulverize with pulverizer under naturalness, then crosses 100 mesh sieves, removes larger particles。Eriocheir sinensis powder and soil are by fixed proportion mixing, and mixed proportion respectively Carapax Eriocheir sinensis ratio accounts for 0.5%, 1.0%, 1.5% 3 ratio of soil。
1.2 lawn plants are cultivated
Experiment is provided with 4 process altogether: respectively 1 matched group (CK), is not added with Carapax Eriocheir sinensis powder;3 Carapax Eriocheir sinensis process groups, add Carapax Eriocheir sinensis powder in mass ratio, process level respectively 0.5%, 1.0%, 1.5%, and each process repeats 3 times respectively, amounts to 12 Culture basins。Experiment container is the little Culture basin of upper diameter 6.9cm, lower diameter 4.2cm, high 8.0cm。Carapax Eriocheir sinensis powder and soil being mixed in proportion, then load mixed-matrix 150g in each container, wherein matched group only adds 150g soil。Selecting the ryegrass seed of full seed, grass seeds is clear water immersion 24 hours under dark condition in advance, put into 150 ryegrass seeds in each Culture basin。Indoor temperature is about 15~27 DEG C, and relative humidity is 43%~64%, is put in laboratory table by lawn plant and cultivates, and carries out natural light irradiation and frequent reversing of position, keeps illumination consistent。Quantitatively sooner or later feedwater every day during plantation, moisture required during to fully ensure that plant growing。
1.3 methods of production
When after planting, 5d carries out the measurement of first time plant height, for surveying the dynamic change of its plant height, measures once every 5 days afterwards, and 4 plant heights surveyed altogether by one batch of grass, cradle after 20d, continue to cultivate growth, cradle, and measure eldish plant height after eldish growth 15d。During mensuration, random in each basin choose the plant that 5 strain growing ways are more well-balanced, take its average plant height as measured value on the same day, measuring ground biomass fresh weight, sample is placed in baking oven, after 108 DEG C of 20min that complete, 80 DEG C of drying start to survey the dry weight of ground biomass to constant weight, and keep a record。
Data all adopt Excel2007 and SPSS17.0 to carry out correlation statistical analysis。
2 development results analyses
2.1 rye grass plant heights
Under different proportion Carapax Eriocheir sinensis powder processes, the growth of rye grass plant height is in Table 1。Analyzing according to table 1, plant, after 20d, is carried out cradling for the first time, after eldish regrowth 15d, carries out the measurement of plant height by plant growing。The process group plant height of interpolation Carapax Eriocheir sinensis relatively compares and significantly improves, along with plant plant height facilitation is had reinforcement trend by the rising of Carapax Eriocheir sinensis powder ratio, compared with the control, the eldish plant height of Carapax Eriocheir sinensis powder process group 0.5%, 1.0% and 1.5% adds 24.43%, 36.17% and 36.89% respectively, and the Carapax Eriocheir sinensis powder process group that ratio is 1.0% and 1.5% on rye grass plant height to affect difference notable, wherein plant plant height facilitation is reached maximum by 1.5% process group。Show that the plant height of eldish is had facilitation by Carapax Eriocheir sinensis powder。Similar to this experimental studies results, illustrate that in this experiment, the facilitation of rye grass plant height is likely due in Carapax Eriocheir sinensis containing organic impacts such as chitins by Carapax Eriocheir sinensis powder, and these Organic substances have played certain fertility in the plant growing later stage;
Table adds the impact (cm) on rye grass plant height of the Carapax Eriocheir sinensis powder in 135 days
2.2 rye grass ground biomass
The Carapax Eriocheir sinensis powder of different proportion processes the impact on rye grass Biomass in Table 2。It can be seen that relative to matched group, one batch of careless ground biomass is all had certain promotion by different proportion Carapax Eriocheir sinensis powder, when Carapax Eriocheir sinensis powder ratio is 1.0%, reaching maximum to its Biomass facilitation, compared with the control, fresh weight, dry weight have been respectively increased 24.71% and 52.08%。The Carapax Eriocheir sinensis powder applying different proportion is all not notable on the fresh weight of one batch of ground biomass of rye grass, dry weight impact。
The applying of Carapax Eriocheir sinensis powder has promoted the accumulation of the ground biomass fresh weight of rye grass eldish, dry weight, along with the facilitation to Plant aboveground Biomass fresh weight, dry weight that raises of Carapax Eriocheir sinensis powder ratio is all strengthened to some extent, compared to comparison, the eldish fresh weight of process group 0.5%, 1.0% and 1.5% adds 66.67%, 109% and 109% respectively, dry weight adds 30%, 80% and 70% respectively, when Carapax Eriocheir sinensis powder ratio is 1.0% and 1.5%, facilitation is notable, it was shown that rye grass eldish ground biomass is respectively provided with facilitation by each ratio of Carapax Eriocheir sinensis powder。
Table 2 different proportion Carapax Eriocheir sinensis powder impact (the g ware on rye grass Biomass-1)
3 develop conclusion
Adding the Carapax Eriocheir sinensis powder of different proportion in rye grass substrate, the fresh weight of rye grass ground biomass is significantly improved by Carapax Eriocheir sinensis powder with dry weight, and especially the dry weight of eldish assumes a marked difference。Additionally, add Carapax Eriocheir sinensis powder can also promote the growth of plant plant height, especially eldish plant height is remarkably promoted effect。
Detailed description of the invention:
The present invention is described below by specific embodiment。Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art。It addition, embodiment is interpreted as illustrative, and unrestricted the scope of the present invention, the spirit and scope of the invention are limited only by the claims that follow。To those skilled in the art, under the premise without departing substantially from spirit and scope of the present invention, the various changes or the changes that carry out the material component in these embodiments and consumption fall within protection scope of the present invention。
Embodiment 1
(1) the lawn plant English ryegrass (LoliumperenneL) selecting northern China relatively common is experiment material, mass of 1000 kernel 3.17g。Being taken from Tianjin Normal University campus the topsoil of sample plot 20cm for examination soil, the soil texture is sandy soil, and pH is about 7.44, the content of organic matter is 4.68%, and total nitrogen content is 0.21%, available phosphorus content 22.03mg/kg, saturation moisture content is 0.58mL/g, and unit weight is 0.87g/cm3。Carapax Eriocheir sinensis selects sea-crab, is first cleaned up by Carapax Eriocheir sinensis, dries, pulverize with pulverizer under naturalness, then crosses 100 mesh sieves, removes larger particles。Eriocheir sinensis powder and soil are by fixed proportion mixing, and mixed proportion respectively Carapax Eriocheir sinensis ratio accounts for 0.5%, 1.0%, 1.5% 3 ratio of soil。
(2) Carapax Eriocheir sinensis powder (1.5%) is added in mass ratio。Experiment container is the little Culture basin of upper diameter 6.9cm, lower diameter 4.2cm, high 8.0cm。Carapax Eriocheir sinensis powder and soil being mixed in proportion, then load mixed-matrix 150g in each container, wherein matched group only adds 150g soil。Selecting the ryegrass seed of full seed, grass seeds is clear water immersion 24 hours under dark condition in advance, put into 150 ryegrass seeds in each Culture basin。Indoor temperature is about 20 DEG C, and relative humidity is 55%, is put in laboratory table by lawn plant and cultivates, and carries out natural light irradiation and frequent reversing of position, keeps illumination consistent。Quantitatively sooner or later feedwater every day during plantation, moisture required during to fully ensure that plant growing。
(3) when after planting 5d carries out the measurement of first time plant height, for surveying the dynamic change of its plant height, measuring once every 5 days afterwards, 4 plant heights surveyed altogether by one batch of grass, cradle after 20d, continue to cultivate growth, cradle, and measure eldish plant height after eldish growth 15d。During mensuration, random in each basin choose the plant that 5 strain growing ways are more well-balanced, take its average plant height as measured value on the same day, measuring ground biomass fresh weight, sample is placed in baking oven, after 108 DEG C of 20min that complete, 80 DEG C of drying start to survey the dry weight of ground biomass to constant weight, and keep a record。Data all adopt Excel2007 and SPSS17.0 to carry out correlation statistical analysis。
(4) conclusion:
Adding the Carapax Eriocheir sinensis powder of different proportion in rye grass substrate, the fresh weight of rye grass ground biomass is significantly improved by Carapax Eriocheir sinensis powder with dry weight, and especially the dry weight of eldish assumes a marked difference。Additionally, add Carapax Eriocheir sinensis powder can also promote the growth of plant plant height, especially eldish plant height is remarkably promoted effect。

Claims (3)

1. Carapax Eriocheir sinensis powder application in improving rye grass ground biomass;Wherein the addition of Carapax Eriocheir sinensis powder is 1-1.5%(W/w)。
2. Carapax Eriocheir sinensis powder application in promoting rye grass plant strain Seedling height。
3. Carapax Eriocheir sinensis powder application in promoting rye grass eldish dry weight。
CN201610065304.5A 2016-02-01 2016-02-01 Method for improving ryegrass growth by adding crab shell powder in matrix Pending CN105684701A (en)

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004352602A (en) * 2003-05-26 2004-12-16 Yamaei:Kk Useful indigenous bacteria-containing organic fertilizer and method for manufacturing the same
CN102630473A (en) * 2012-05-04 2012-08-15 天津师范大学 Setting method for nitrilotriacetic acid (NTA) recovered garbage compost heavy metal permeability-reducing spacing layer
CN103539500A (en) * 2013-10-15 2014-01-29 蔡水花 Composite fertilizer and preparation method thereof
US20140274691A1 (en) * 2013-03-15 2014-09-18 Spogen Biotech Inc. Plant growth-promoting bacteria and methods of use
CN104312592A (en) * 2014-09-19 2015-01-28 大连珍奥生物农业科技股份有限公司 Preparation method of soil conditioner
CN105087016A (en) * 2015-09-09 2015-11-25 南宁汉和生物科技有限公司 Soil conditioner rich in marine substances
CN105175183A (en) * 2015-09-01 2015-12-23 刘奋安 Multifunctional soil conditioning agent adopting shrimp and crab shell as main material, and preparation method thereof
CN105254370A (en) * 2015-11-02 2016-01-20 大新县生产力促进中心 Dendrobium officinale leaf fertilizer and preparation method thereof

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004352602A (en) * 2003-05-26 2004-12-16 Yamaei:Kk Useful indigenous bacteria-containing organic fertilizer and method for manufacturing the same
CN102630473A (en) * 2012-05-04 2012-08-15 天津师范大学 Setting method for nitrilotriacetic acid (NTA) recovered garbage compost heavy metal permeability-reducing spacing layer
US20140274691A1 (en) * 2013-03-15 2014-09-18 Spogen Biotech Inc. Plant growth-promoting bacteria and methods of use
CN103539500A (en) * 2013-10-15 2014-01-29 蔡水花 Composite fertilizer and preparation method thereof
CN104312592A (en) * 2014-09-19 2015-01-28 大连珍奥生物农业科技股份有限公司 Preparation method of soil conditioner
CN105175183A (en) * 2015-09-01 2015-12-23 刘奋安 Multifunctional soil conditioning agent adopting shrimp and crab shell as main material, and preparation method thereof
CN105087016A (en) * 2015-09-09 2015-11-25 南宁汉和生物科技有限公司 Soil conditioner rich in marine substances
CN105254370A (en) * 2015-11-02 2016-01-20 大新县生产力促进中心 Dendrobium officinale leaf fertilizer and preparation method thereof

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