CN105675816B - The horizontal mound layering seedling root monitoring method of apple rootstock - Google Patents
The horizontal mound layering seedling root monitoring method of apple rootstock Download PDFInfo
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- CN105675816B CN105675816B CN201610060229.3A CN201610060229A CN105675816B CN 105675816 B CN105675816 B CN 105675816B CN 201610060229 A CN201610060229 A CN 201610060229A CN 105675816 B CN105675816 B CN 105675816B
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Abstract
The present invention relates to a kind of method of researching plant root Growth trends situation, the specially horizontal mound layering seedling root growth monitoring method of apple rootstock, annual apple rootstock is transplanted in ground in spring level, the sprout of plant back is handled to promote its sprouting before stock non-rudiment, when young sprout length is to 15cm, retain it is quantitative it is suitable it is new slightly, and the horizontal setting mini-rhizotron between stock, sawdust is covered, afterwards with condition of rooting during the horizontal mound layering of root scanner periodic monitoring apple rootstock and later stage root growth dynamic.The horizontal mound layering seedling root growth monitoring method of apple rootstock provided by the invention, seedling root Growth trends situation during the research horizontal mound layering of apple rootstock, observation and the quick and precisely measurement of morphological parameters directly perceived to seedling root and analysis are realized, provides one kind directly perceived, efficient, reliable research meanses to filter out the high apple rootstock of breeding coefficient during horizontal mound layering.
Description
Technical field
The present invention relates to a kind of method of researching plant root Growth trends situation, the specially horizontal press strip of apple rootstock are numerous
Grow seedling root growth monitoring method.
Background technology
Mound layering is a kind of common method of fruit nursery stock production, and clone seedling can be obtained using mound layering
The excellent stock of wood, especially fruit tree mainly leans on clonal reproduction.The generation of lateral root, root growth hair during mound layering
Educate situation and rooting rate number be the key for evaluating mound layering success or not.Because mound layering root growth is in covering
Under, therefore for root system progress is accurately observed, measure has certain difficulty during mound layering.Traditional fruit tree press strip
It is mining method, monolith method, profile method etc. to breed root system observation procedure, but these methods are respectively provided with sampling destructiveness and work
Measure the shortcomings of big, and the method is typically carried out in press strip growth latter stage, is unfavorable for evaluation of the early stage to mound layering characteristic.Therefore
Cause during fruit tree mound layering root system management not in time, root growth data obtain not system etc..
The content of the invention
For above-mentioned technical problem, the present invention provides seedling root during a kind of research horizontal mound layering of apple rootstock
Growth trends observation procedure, quick and precisely measurement and analysis to root growth morphological parameters are realized, to filter out horizontal pressure
The Dwarf Stocks For Apple Trees that breeding coefficient is high in bar reproductive process provide a kind of directly perceived, efficient, reliable research meanses.Particular technique
Scheme is：
The horizontal mound layering seedling root growth monitoring method of apple rootstock, comprises the following steps：
(1) horizontal setting mini-rhizotron
It is close to apple press strip maternal plant and digs a deep 3cm, wide 8cm, long 1m shallow ridges, the 1m mini-rhizotrons grown then is put in ditch
In, and mini-rhizotron mouth is filled into the space between mini-rhizotron and shallow ridges, compacting towards maternal plant young sprout top with fine earth；Use U-iron
Silk fixes front end and the end of mini-rhizotron and ensures that the tube wall of mini-rhizotron is close to press strip maternal plant respectively, vertical at the mini-rhizotron mouth of pipe
Mini-rhizotron digs wide 15cm, deep 10cm ditch, to ensure that root scanner can enter mini-rhizotron normal scan；Mini-rhizotron and press strip are female
The thick sawdusts of 10-15cm are covered in strain；
(2) periodic scanning
Since being covered sawdust, first 3 weeks, mini-rhizotron was scanned 1 time using Rootscanner-R root systems observation system every 1 week,
Afterwards every 2 days run-downs, until finding new root, and rootage duration is recorded；It was found that being scanned 1 time every 7 days after new root, see
Examine the root growth dynamic of mound layering nursery stock；
(3) scanned picture is analyzed
After obtaining root system scanned picture, using the horizontal press strip nursery stock new root time of origin of root system analysis software, take root
Rate, and the root system overall length increment of different growing stage, total surface area increment, cumulative volume increment, overall average rugosity increase
The data of growing such as long amount.
The horizontal mound layering seedling root growth monitoring method of Dwarf Stocks For Apple Trees provided by the invention, study apple dwarf
Root growth dynamic situation during the horizontal mound layering of stock, realize quick, the accurate survey to root growth morphological parameters
Amount and analysis, for filter out the high Dwarf Stocks For Apple Trees of breeding coefficient during horizontal mound layering provides it is a kind of it is directly perceived, efficient,
Reliable research meanses.
Embodiment
It is described in conjunction with the embodiments the embodiment of the present invention.
To Dwarf Stocks For Apple Trees SH40 offsprings No. 9-3, No. 111, No. 3-45, No. 3-7-15-1, No. 36, BP, No. 2, carry out
Horizontal mound layering.The horizontal mound layering Root System Growth Characteristics of Dwarf Stocks For Apple Trees are carried out with the inventive method to evaluate, evaluation
Index includes rootage duration, rooting rate, the overall length increments of different times, total surface area increment, cumulative volume increment, total flat
Equal rugosity increment etc..
The method that root growth dynamic situation during the horizontal mound layering of Dwarf Stocks For Apple Trees is monitored using mini-rhizotron method,
Comprise the following steps：
(1) horizontal setting mini-rhizotron
It is close to apple press strip maternal plant and digs a deep 3cm, wide 8cm, long 1m shallow ridges, the 1m mini-rhizotrons grown then is put in ditch
In, and mini-rhizotron mouth is had into the space between mini-rhizotron and shallow ridges, compacting towards maternal plant young sprout top with fine earth landfill；Use U-shaped
Iron wire fixes front end and the end of mini-rhizotron and ensures that the tube wall of mini-rhizotron is close to press strip maternal plant respectively, is hung down at the mini-rhizotron mouth of pipe
Straight mini-rhizotron digs wide 15cm, deep 10cm ditch, to ensure that root scanner can enter mini-rhizotron normal scan；Mini-rhizotron and press strip
The thick sawdusts of 10-15cm are covered on maternal plant；
(2) periodic scanning
Since being covered sawdust, first 3 weeks, mini-rhizotron was scanned 1 time using Rootscanner-R root systems observation system every 1 week,
Afterwards every 3 days run-downs, until finding new root, and rootage duration is recorded；It was found that being scanned 1 time every 7 days after new root, see
Examine the root growth dynamic of mound layering nursery stock；
(3) scanned picture is analyzed
After obtaining root system scanned picture, using the horizontal press strip nursery stock new root time of origin of root system analysis software, take root
Rate, and the root system overall length increment of different growing stage, total surface area increment, cumulative volume increment, overall average rugosity increase
The data of growing such as long amount.
The horizontal mound layering evaluating characteristics of Dwarf Stocks For Apple Trees have been carried out with this method, the results are shown in Table 1 and table 2；Show not
With Dwarf Stocks For Apple Trees, difference is obvious in terms of rootage duration, rooting rate；Compared with the control group of conventional conventional method, this implementation
Example is substantially advanced by the rootage duration of 9-3 stocks, and significantly increases root system overall length, total surface area, cumulative volume and overall average
Rugosity.It is taken as that this method is practical.
The rootage duration and rooting rate of the horizontal mound layering of several Dwarf Stocks For Apple Trees of table 1
| Try material | Rootage duration | Rooting rate |
| 9-3 | Cover after sawdust the 34th day | 80% |
| 3-7-15-1 | Cover after sawdust the 75th day | 40% |
| 3-45 | Cover after sawdust the 75th day | 70% |
| BP | Cover after sawdust the 75th day | 20% |
| 111 | Cover after sawdust the 80th day | 10% |
| 36 | Cover after sawdust the 90th day | 20% |
| No. 2 | Nothing | 0% |
Influence of the different disposal method of table 2 to root growth data during the horizontal press strip of 9-3 stocks
Claims (1)
1. the horizontal mound layering seedling root growth monitoring method of apple rootstock, it is characterised in that comprise the following steps：
(1) horizontal setting mini-rhizotron
It is close to apple press strip maternal plant and digs a deep 3cm, wide 8cm, long 1m shallow ridges, then the 1m mini-rhizotrons grown is put in ditch,
And mini-rhizotron mouth there are into the space between mini-rhizotron and shallow ridges, compacting towards maternal plant young sprout top with fine earth landfill；Use U-iron
Silk fixes front end and the end of mini-rhizotron and ensures that the tube wall of mini-rhizotron is close to press strip maternal plant respectively, vertical at the mini-rhizotron mouth of pipe
Mini-rhizotron digs wide 15cm, deep 10cm ditch, to ensure that root scanner can enter mini-rhizotron normal scan；Mini-rhizotron and press strip are female
The thick sawdusts of 10-15cm are covered in strain；
(2) periodic scanning
Since being covered sawdust, first 3 weeks, scanned mini-rhizotron 1 time using root system observation system every 1 week, scanned one every 3 days afterwards
It is secondary, until finding new root, and record rootage duration；It was found that scanned 1 time every 7 days after new root, observation mound layering nursery stock
Root growth dynamic；
(3) scanned picture is analyzed
After obtaining root system scanned picture, using the horizontal press strip nursery stock new root time of origin of root system analysis software, rooting rate, with
And root system overall length increment, total surface area increment, cumulative volume increment, the overall average rugosity increment of different growing stage are given birth to
Long development data.
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| CN109006315B (en) * | 2018-07-30 | 2021-07-30 | 云南省农业科学院甘蔗研究所 | Method for monitoring growth dynamics of sugarcane root system |
| CN111323536A (en) * | 2020-02-17 | 2020-06-23 | 沈阳农业大学 | Root space expansion quantitative model construction method for rhizome type clone plant |
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| US3967198A (en) * | 1974-09-20 | 1976-06-29 | Gensler William G | Method and apparatus for electrically determining plant water status |
| US7829291B2 (en) * | 2005-02-17 | 2010-11-09 | Vanderbilt University | In situ analysis of tissues |
| CN101216470B (en) * | 2008-01-08 | 2011-05-04 | 上海大学 | Mucky beach plant root system growth in situ monitoring method |
| CN103364399B (en) * | 2013-07-12 | 2015-06-17 | 北京农业信息技术研究中心 | Crop root growth detection system and detection method |
| CN103901164A (en) * | 2014-03-05 | 2014-07-02 | 中国农业大学 | Plant root system vision monitoring and analysis system and monitoring method thereof |
| CN104459045B (en) * | 2014-12-03 | 2016-04-20 | 中国科学院寒区旱区环境与工程研究所 | Precipitation infiltration process and root growth dynamic comprehensive recording geometry |
| CN104982316B (en) * | 2015-06-15 | 2017-05-17 | 中国林业科学研究院林业新技术研究所 | Fixation device and method for in-situ monitoring of floating plant root system |
| CN105160584A (en) * | 2015-08-03 | 2015-12-16 | 中国林业科学研究院林业新技术研究所 | Method and equipment system for in situ dynamic monitoring of wetland plant root system |
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