CN102415249B - Method for quickly evaluating genetic effect of sugarcane parent on ratoon character - Google Patents
Method for quickly evaluating genetic effect of sugarcane parent on ratoon character Download PDFInfo
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- CN102415249B CN102415249B CN2011102328182A CN201110232818A CN102415249B CN 102415249 B CN102415249 B CN 102415249B CN 2011102328182 A CN2011102328182 A CN 2011102328182A CN 201110232818 A CN201110232818 A CN 201110232818A CN 102415249 B CN102415249 B CN 102415249B
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- sugarcane
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- perennial root
- ratoon
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Abstract
The invention discloses a method for quickly evaluating the genetic effect of a sugarcane parent on ratoon character. The method comprises the following steps of: analyzing and calculating the ratoon character combining ability and hereditary capacity of the sugarcane parent by using the characteristics of sugarcane seedling population grown plants before the sugarcane seedling population grown plants are transplanted in field and combining free software R; after seedlings are temporarily planted and grow to obtain seven true leaves, shearing off the ground parts; after the plants are grown, investigating the ratoon plant growing condition of the progeny generation population; and by combining the free software R, researching the genetic effect of the parent on the ratoon character of the progeny generation population. The invention discloses the method for evaluating the genetic effect of the parent on the ratoon character by using the sugarcane seedlings before being transplanted in field. According to the method, the genetic effect of the parent on the ratoon character of the progeny generation can be researched by using the sugarcane seedlings which are not transplanted in the field; the high-efficiency screening of better sugarcane parents having strong genetic effect on the ratoon character is improved; and the efficiency for cultivating novel improved variety of strong ratoon sugarcane is improved.
Description
Technical field:
The present invention relates to sugar cane breed seed selection field, specifically a kind of method of Fast Evaluation Sugarcane Breeding perennial root hereditary effect.
Background technology:
China's Sugar Production is faced with the present situation that supply falls short of demand, and increasing sugarcane acreage is to solve the sugar basic guarantee that supply falls short of demand.It is relatively large that cane planting drops into, and other economic crops are relatively planted the sugarcane effect not very good.In addition, cane planting needs labor intensive larger, along with rural laborer's a large amount of cities transfer, and sugarcane district's quantity of labour force decline.Keep sugarcane acreage not subtract, the most basic measure is to improve the sugarcane grower plant the sugarcane benefit and reduce and plant sugarcane labour input.The seed selection of strong perennial root sugar cane breed is also promoted, and can extend the Leifsonia time limit, reduces and plants sugarcane cost and labour's input.
Because the perennial root sugarcane has advantages of province's kind, economizes the labour.Paid much attention to all the time.But it is not good enough to promote at present the sugar cane breed perennial root, and the perennial root time limit is shorter and a strain ability is relatively poor.Sugarcane grower's planting rate is higher.Therefore, produce the strong perennial root improved Varieties of Sugarcane of urgent need.And sugar cane breed seed selection year limit for length, the seed selection of strong perennial root improved Varieties of Sugarcane needs time of relatively growing and the input of Geng Gao especially.
The parent is the basis of sugar cane breed improvement, grasps the parent to the hereditary effect of offspring's perennial root, is conducive to improve the efficient of strong perennial root improved Varieties of Sugarcane seed selection.Aspect the evaluation of parent's perennial root hereditary effect, the cane breeding person adopts sexual generation land for growing field crops perennial root and the data of planting, in conjunction with coordinate force and the heritability effect of certain Study on Mathematic Model parent to offspring's perennial root, provide foundation thereby match for strong perennial root improved Varieties of Sugarcane hybrid combination.Yet because the field experiment floor space is larger, and the root of must staying overnight could carry out this research, need to be than high investment and limit more all the year round.
Summary of the invention:
The invention provides a kind of method of Fast Evaluation Sugarcane Breeding perennial root hereditary effect, the method utilizes the cane seedlings colony before field-transplanting to send out the strain characteristic, and analyzes in conjunction with the R free software, calculates Sugarcane Breeding perennial root coordinate force and heritability.
This method after it grows to 7 true leaves, cuts off acrial part after seedling is heeled in, after sending out strain, investigation progeny population perennial root is sent out the strain situation, and in conjunction with the R free software, thereby the research parent is to the hereditary effect of progeny population perennial root.
The present invention is the method for utilizing the front cane seedlings evaluation parent perennial root hereditary effect of field-transplanting, and the method can be utilized the cane seedlings of not transplanting the land for growing field crops, the hereditary effect of research parent to offspring's perennial root.Improve the efficient screening to the better parent of the strong perennial root hereditary effect of sugarcane, improved the efficient of breeding strong perennial root improved Varieties of Sugarcane.
Embodiment:
A kind of method of Fast Evaluation Sugarcane Breeding perennial root hereditary effect, implementation step is as follows in detail:
1, obtain cane seedlings: obtain test hybrid seed used according to breeding objective Parented selected mating of hybridization and hybridization, test hybrid seed used is seeded in the container that the cane seedlings culture matrix is housed, control 22-36 ℃ of matrix indoor temperature, and keep matrix moist, begin the extermination of disease and insect pest in good time, can obtain cane seedlings.
2, cane seedlings is heeled in: after cane seedlings grows to 2 true leaves, it is carried out heel in.Seedling is heeled in to nutritious bag, and each combination offspring divides triple multiple cropping to plant.
3, cane seedlings perennial root investigation: after the cane seedlings after heeling in grows to 7 true leaves, select without rainy weather, cut off the above part of seedling growth point.Wait cutting after rear seedling colony sends out strain and finish, with the every repetitive sequence investigation of each combination progeny population more than 30 clumps seedling send out the strain data.
4, parent's perennial root hereditary effect is estimated: collect according to table 1 form and send out the strain data rows,
Sprouting sends out the strain average in repeating
Adopt the R free software to calculate coordinate force and specific combining ability, maternal, male parent is as follows to progeny population perennial root combining ability effect value calculation procedure to offspring's perennial root combining ability effect value and combination:
Rationing＜-read.csv (" E :/34.csv ") # reading out data is to ratooning
Str (rationing) # shows the ratooning data construction
Rationing $ pltratn＜-factor (rationing $ block) # definition block (repetition) is the factor
Library (lme4) # loads the lme4 model
Sprouting＜-lmer (sprouting～block+ (1|female), rationing) # as female parent to offspring's perennial root combining ability effect
ranef(sprouting)
Sprouting＜-lmer (sprouting～block+ (1|male), rationing) # as male parent to offspring's perennial root combining ability effect
ranef(sprouting)
Sprouting＜-lmer (sprouting～block+ (1|cross), rationing) the special compounding stress effect of # hybrid combination to offspring's perennial root
ranef(sprouting)
Beneficial effect:
The present invention adopts a strain characteristic of the front cane seedlings of field-transplanting, study in conjunction with coordinate force and heritability effect that R free software research Sugarcane Breeding is sent out the strain characteristic to the progeny population perennial root, relatively adopt land for growing field crops planting perennial root data research Sugarcane Breeding to the hereditary effect of offspring's perennial root, can save in a large number the parent and estimate needed time and test input.The method can be served cane breeding mechanism, is used for screening Sugarcane Breeding and the germ plasm resource good to the progeny population hereditary effect.
Claims (2)
1. the method for a Fast Evaluation Sugarcane Breeding perennial root hereditary effect is characterized in that step is as follows:
(1), obtain cane seedlings: obtain test hybrid seed used according to breeding objective Parented selected mating of hybridization and hybridization, test hybrid seed used is seeded in the container that the cane seedlings culture matrix is housed, control 22-36 ℃ of matrix indoor temperature, and keep matrix moist, begin the extermination of disease and insect pest in good time, can obtain cane seedlings;
(2), cane seedlings is heeled in: after cane seedlings grows to 2 true leaves, it is carried out heel in, seedling is heeled in to nutritious bag, each combination offspring divides triple multiple cropping to plant;
(3), cane seedlings perennial root investigation: after the cane seedlings after heeling in grows to 7 true leaves, cut off the above part of seedling growth point, wait cutting after rear seedling colony sends out strain and finish, with the every repetitive sequence investigation of each combination progeny population more than 30 clumps seedling send out the strain data; According to a strain data of seedling, in conjunction with the R free software, calculate the parent and the progeny population perennial root is sent out the hereditary effect of strain.
2. the method for Fast Evaluation Sugarcane Breeding perennial root hereditary effect according to claim 1, it is characterized in that adopting the R free software to calculate coordinate force and specific combining ability, maternal, male parent is as follows to progeny population perennial root combining ability effect value calculation procedure to offspring's perennial root combining ability effect value and combination:
Rationing＜-read.csv (" E :/34.csv ") # reading out data is to ratooning,
Str (rationing) # shows the ratooning data construction,
Rationing $ pltratn＜-factor (rationing $ block) # definition block(repetition) be the factor,
Library (lme4) # loads the lme4 model,
Sprouting＜-lmer (sprouting ~ block+ (1|female), rationing) # as female parent to offspring's perennial root combining ability effect,
ranef(sprouting)，
Sprouting＜-lmer (sprouting ~ block+ (1|male), rationing) # as male parent to offspring's perennial root combining ability effect,
ranef(sprouting)，
Sprouting＜-lmer (sprouting ~ block+ (1|cross), rationing) the special compounding stress effect of # hybrid combination to offspring's perennial root,
ranef(sprouting)。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102328182A CN102415249B (en) | 2011-08-15 | 2011-08-15 | Method for quickly evaluating genetic effect of sugarcane parent on ratoon character |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102328182A CN102415249B (en) | 2011-08-15 | 2011-08-15 | Method for quickly evaluating genetic effect of sugarcane parent on ratoon character |
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| CN102415249A CN102415249A (en) | 2012-04-18 |
| CN102415249B true CN102415249B (en) | 2013-06-19 |
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| CN2011102328182A Expired - Fee Related CN102415249B (en) | 2011-08-15 | 2011-08-15 | Method for quickly evaluating genetic effect of sugarcane parent on ratoon character |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104472347B (en) * | 2014-11-06 | 2018-01-19 | 瑞丽滇蔗农业科技开发有限公司 | A kind of strong ratoon sugarcane crosses screening technique |
| CN105660099B (en) * | 2016-01-12 | 2018-05-15 | 广西南亚热带农业科学研究所 | A kind of culturing sugarcane seedlings method |
| CN106688655A (en) * | 2016-11-30 | 2017-05-24 | 云南省农业科学院甘蔗研究所 | Simple method for evaluating sugarcane hybridized combinations |
| CN107674909A (en) * | 2017-11-17 | 2018-02-09 | 云南省农业科学院甘蔗研究所 | A kind of method of Fast Evaluation Sugarcane Breeding leaf rust genetics of resistance effect |
| CN109618696B (en) * | 2018-12-26 | 2020-10-20 | 广西壮族自治区农业科学院甘蔗研究所 | Method for 3-time repeated transplanting and rapid breeding of sugarcane healthy seedling stock seedlings |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JPS63313532A (en) * | 1986-12-04 | 1988-12-21 | ディー・エヌ・エイ・プラント・テクノロジー・コーポレイション | Method for cutting gene chain |
| CN101496495A (en) * | 2008-12-22 | 2009-08-05 | 云南省农业科学院甘蔗研究所 | Comprehensive evaluation method for novel stain of sugarcane |
| CN101427655A (en) * | 2008-12-22 | 2009-05-13 | 云南省农业科学院甘蔗研究所 | Multi-purpose evaluating method for sugarcane family |
| CN101933438A (en) * | 2010-09-16 | 2011-01-05 | 云南省农业科学院甘蔗研究所 | Sugarcane lateral branch rapid reproduction technology |
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